[{"intvolume":" 48","publication":"Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere","doi":"10.1055/a-1238-1554","_id":"12955","user_id":"83781","keyword":["Hund - Algeninfektion - chronischer Durchfall - Protothekose - Prototheca zopfii"],"page":"369-375","language":[{"iso":"ger"}],"citation":{"ama":"Geisen V, Mayer C, Harrer J, Hartmann K, Ulrich S, Unterer S. Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere. 2020;48(05):369-375. doi:10.1055/a-1238-1554","van":"Geisen V, Mayer C, Harrer J, Hartmann K, Ulrich S, Unterer S. Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere. 2020;48(05):369–75.","bjps":"Geisen V et al. (2020) Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 48, 369–375.","din1505-2-1":"Geisen, Vera ; Mayer, Christian ; Harrer, Julia ; Hartmann, Katrin ; Ulrich, Sebastian ; Unterer, Stefan: Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. In: Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere Bd. 48. Stuttgart, Thieme (2020), Nr. 05, S. 369–375","ufg":"Geisen, Vera u. a.: Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland, in: Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 48 (2020), H. 05, S. 369–375.","short":"V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, S. Unterer, Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 48 (2020) 369–375.","chicago":"Geisen, Vera, Christian Mayer, Julia Harrer, Katrin Hartmann, Sebastian Ulrich, and Stefan Unterer. “Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland.” Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 48, no. 05 (2020): 369–75. https://doi.org/10.1055/a-1238-1554.","mla":"Geisen, Vera, et al. “Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland.” Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, vol. 48, no. 05, 2020, pp. 369–75, https://doi.org/10.1055/a-1238-1554.","havard":"V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, S. Unterer, Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland, Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere. 48 (2020) 369–375.","chicago-de":"Geisen, Vera, Christian Mayer, Julia Harrer, Katrin Hartmann, Sebastian Ulrich und Stefan Unterer. 2020. Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 48, Nr. 05: 369–375. doi:10.1055/a-1238-1554, .","apa":"Geisen, V., Mayer, C., Harrer, J., Hartmann, K., Ulrich, S., & Unterer, S. (2020). Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, 48(05), 369–375. https://doi.org/10.1055/a-1238-1554","ieee":"V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, and S. Unterer, “Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland,” Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, vol. 48, no. 05, pp. 369–375, 2020, doi: 10.1055/a-1238-1554."},"publisher":"Thieme ","extern":"1","status":"public","publication_identifier":{"issn":["1434-1239"],"eissn":["2567-5842"]},"date_created":"2025-06-15T10:01:12Z","volume":48,"publication_status":"published","date_updated":"2025-06-18T12:24:09Z","department":[{"_id":"DEP4010"}],"abstract":[{"lang":"ger","text":"Ein 10 Monate alter, männlich intakter Rhodesian Ridgeback wurde wegen chronischen Dickdarmdurchfalls und Hämatochezie vorgestellt. Der Hund stammte aus Deutschland und hatte das Land nie verlassen. Die Laboruntersuchung des vorbehandelnden Tierarztes ergab neben einer Neutrophilie eine Hyperkaliämie und eine Hyponatriämie. Mit einem Serumbasalkortisolwert von 4,3 µg/dl konnte ein Hypoadrenokortizismus weitgehend ausgeschlossen werden. Eine vom Tierarzt durch geführte antibiotische Behandlung hatte keine Besserung bewirkt. Daher war der Hund mit Prednisolon behandelt worden. Unter 2-wöchiger Prednisolongabe kam es zu einer deutlichen Verstärkung des Durchfalls sowie einem Gewichtsverlust von 6 kg. Bei Vorstellung an der Medizinischen Kleintierklinik der LMU München war der Hund im Allgemeinbefinden mittelgradig reduziert, deutlich abgemagert, dehydriert, hypovolämisch und hatte eine rektale Körpertemperatur von 39,6 °C. Bei der sonografischen Untersuchung zeigte sich eine generalisiert verdickte Dickdarmwand und koloskopisch eine hochgradig ulzerativ veränderte Dickdarmschleimhaut. Histologischer Befund war eine ulzerative granulomatöse Kolitis. Durch die Periodic-Acid-Schiff-Reaktion ließen sich in den Schnitten der Dickdarmbioptate mikrobielle Strukturen darstellen, die für eine Algeninfektion diagnostisch waren. Die bei der mikrobiellen Untersuchung anzüchtbaren Prototheken wurden mittels MALDI-TOF-MS als Prototheca zopfii identifiziert. Zum Nachweis einer möglichen Immundefizienz wurden die Immunglobuline im Serum bestimmt. Die IgM-Konzentration war erniedrigt, während sich IgG- und IgA-Konzentration im Referenzbereich befanden. Aufgrund der Verschlechterung des Allgemeinbefindens, der vorsichtigen Prognose und der hohen Kosten eines Therapieversuchs wurde der Hund eine Woche später euthanasiert und der Tierkörper pathologisch untersucht. Histopathologisch wurden Prototheken auch in den abdominalen Lymphknoten, jedoch nicht in den Augen oder im zentralen Nervensystem identifiziert. Der Fall zeigt, dass eine Prototheken-Infektion auch bei Hunden aus Deutschland als Differenzialdiagnose für chronischen Dickdarmdurchfall in Betracht gezogen werden sollte, insbesondere bei Patienten mit ulzerativer granulomatöser Kolitis. Sie kann bei der histologischen Untersuchung ohne Spezialfärbung leicht übersehen werden."},{"lang":"eng","text":"A 10-month-old male Rhodesian Ridgeback was presented to the Clinic of Small Animal Medicine, LMU, Germany, with a 6-month history of chronic diarrhea and hematochezia. The dog lived in Germany and had never traveled abroad. Complete blood count and serum biochemistry performed by the referring veterinarian revealed neutrophilia, hyperkalemia, and hyponatremia, with a basal cortisol of 4.3 µg/dl, which excluded hypoadrenocorticism. Since antibiotic treatment had not resulted in any improvement, a 2 week course of prednisolone administration had been initiated, leading to a marked deterioration of intestinal signs and a significant weight loss of 6 kg. At the time of referral, the patient was markedly emaciated, dehydrated, hypovolemic and had a rectal temperature of 39.6 °C. Abdominal ultrasound showed a thickened and irregular colonic wall. On colonoscopy, an irregular colonic mucosa with ulcerations was observed. Histopathologic examination revealed an ulcerative granulomatous colitis, and on Periodic acid-Schiff reaction (PAS) numerous organisms consistent with Prototheca spp. were identified. Prototheca zopfii infection was confirmed by culture and MALDI-TOF MS. In order to test for an underlying immunodeficiency, immunoglobulin levels in serum were determined. IgM was decreased, while IgG and IgA levels were within the reference interval. Due to deterioration of general condition, grave prognosis and costs of a treatment trial, the patient was euthanized one week later, and necropsy was performed. Prototheca spp. were detected on histopathologic examination in the lymphnodes, however not in the eyes or the central nervous system. Protothecosis should be considered an differential diagnosis in dogs with chronic diarrhea and ulcerative granulomatous colitis even in dogs living in Germany. Histopathologic examination of colonic biopsies with special stains such as PAS is recommended in every dog with signs of chronic large bowel disease in order to avoid missing this rare infectious disease."}],"year":"2020","title":"Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland","issue":"05","author":[{"first_name":"Vera","full_name":"Geisen, Vera","last_name":"Geisen"},{"first_name":"Christian","full_name":"Mayer, Christian","last_name":"Mayer"},{"first_name":"Julia","last_name":"Harrer","full_name":"Harrer, Julia"},{"full_name":"Hartmann, Katrin","last_name":"Hartmann","first_name":"Katrin"},{"first_name":"Sebastian","full_name":"Ulrich, Sebastian","id":"85847","orcid":"0000-0002-4511-9537","last_name":"Ulrich"},{"first_name":"Stefan","last_name":"Unterer","full_name":"Unterer, Stefan"}],"place":"Stuttgart","type":"scientific_journal_article"},{"quality_controlled":"1","date_created":"2025-06-15T10:01:42Z","publication_identifier":{"eissn":["2309-608X"]},"date_updated":"2025-06-16T13:59:56Z","publication_status":"published","volume":6,"year":"2020","abstract":[{"text":"Stachybotrys (S.) chartarum had been linked to severe health problems in humans and animals, which occur after exposure to the toxic secondary metabolites of this mold. S. chartarum had been isolated from different environmental sources, ranging from culinary herbs and improperly stored fodder to damp building materials. To access the pathogenic potential of isolates, it is essential to analyze them under defined conditions that allow for the production of their toxic metabolites. All Stachybotrys species are assumed to produce the immunosuppressive phenylspirodrimanes, but the highly cytotoxic macrocyclic trichothecenes are exclusively generated by the genotype S of S. chartarum. In this study, we have analyzed four genotype S strains initially isolated from three different habitats. We grew them on five commonly used media (malt-extract-agar, glucose-yeast-peptone-agar, potato-dextrose-agar, cellulose-agar, Sabouraud-dextrose-agar) to identify conditions that promote mycotoxin production. Using LC-MS/MS, we have quantified stachybotrylactam and all S-type specific macrocyclic trichothecenes (satratoxin G, H, F, roridin E, L-2, verrucarin J). All five media supported a comparable fungal growth and sporulation at 25 °C in the dark. The highest concentrations of macrocyclic trichothecenes were detected on potato-dextrose-agar or cellulose-agar. Malt-extract-agar let to an intermediate and glucose-yeast-peptone-agar and Sabouraud-dextrose-agar to a poor mycotoxin production. These data demonstrate that the mycotoxin production clearly depends on the composition of the respective medium. Our findings provide a starting point for further studies in order to identify individual components that either support or repress the production of mycotoxins in S. chartarum.","lang":"eng"}],"department":[{"_id":"DEP4010"}],"place":"Basel","type":"scientific_journal_article","issue":"3","title":"Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media","author":[{"id":"85847","full_name":"Ulrich, Sebastian","orcid":"0000-0002-4511-9537","last_name":"Ulrich","first_name":"Sebastian"},{"last_name":"Schäfer","full_name":"Schäfer, Cornelius","first_name":"Cornelius"}],"intvolume":" 6","publication":"Journal of Fungi","doi":"10.3390/jof6030159","publisher":"MDPI ","language":[{"iso":"eng"}],"citation":{"short":"S. Ulrich, C. Schäfer, Journal of Fungi 6 (2020).","ufg":"Ulrich, Sebastian/Schäfer, Cornelius: Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media, in: Journal of Fungi 6 (2020), H. 3.","ieee":"S. Ulrich and C. Schäfer, “Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media,” Journal of Fungi, vol. 6, no. 3, Art. no. 159, 2020, doi: 10.3390/jof6030159.","apa":"Ulrich, S., & Schäfer, C. (2020). Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media. Journal of Fungi, 6(3), Article 159. https://doi.org/10.3390/jof6030159","chicago-de":"Ulrich, Sebastian und Cornelius Schäfer. 2020. Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media. Journal of Fungi 6, Nr. 3. doi:10.3390/jof6030159, .","havard":"S. Ulrich, C. Schäfer, Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media, Journal of Fungi. 6 (2020).","din1505-2-1":"Ulrich, Sebastian ; Schäfer, Cornelius: Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media. In: Journal of Fungi Bd. 6. Basel, MDPI (2020), Nr. 3","bjps":"Ulrich S and Schäfer C (2020) Toxin Production by Stachybotrys Chartarum Genotype S on Different Culture Media. Journal of Fungi 6.","van":"Ulrich S, Schäfer C. Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media. Journal of Fungi. 2020;6(3).","mla":"Ulrich, Sebastian, and Cornelius Schäfer. “Toxin Production by Stachybotrys Chartarum Genotype S on Different Culture Media.” Journal of Fungi, vol. 6, no. 3, 159, 2020, https://doi.org/10.3390/jof6030159.","ama":"Ulrich S, Schäfer C. Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media. Journal of Fungi. 2020;6(3). doi:10.3390/jof6030159","chicago":"Ulrich, Sebastian, and Cornelius Schäfer. “Toxin Production by Stachybotrys Chartarum Genotype S on Different Culture Media.” Journal of Fungi 6, no. 3 (2020). https://doi.org/10.3390/jof6030159."},"keyword":["Stachybotrys","genotype","macrocyclic trichothecenes","stachybotrylactam"],"_id":"12956","user_id":"83781","extern":"1","status":"public","article_number":"159"},{"publication":"Parasites & vectors","doi":"10.1186/s13071-020-04060-y","intvolume":" 13","keyword":["Lyme borreliosis","Borrelia burgdorferi","Tick-borne relapsing fever","Borrelia persica","Blood clearance"],"_id":"12962","user_id":"83781","citation":{"mla":"Liang, Liucun, et al. “Rapid Clearance of Borrelia Burgdorferi from the Blood Circulation.” Parasites & Vectors, vol. 13, no. 1, 191, 2020, https://doi.org/10.1186/s13071-020-04060-y.","chicago":"Liang, Liucun, Jinyong Wang, Lucas Schorter, Thu Phong Nguyen Trong, Shari Fell, Sebastian Ulrich, and Reinhard K. Straubinger. “Rapid Clearance of Borrelia Burgdorferi from the Blood Circulation.” Parasites & Vectors 13, no. 1 (2020). https://doi.org/10.1186/s13071-020-04060-y.","ieee":"L. Liang et al., “Rapid clearance of Borrelia burgdorferi from the blood circulation,” Parasites & vectors, vol. 13, no. 1, Art. no. 191, 2020, doi: 10.1186/s13071-020-04060-y.","apa":"Liang, L., Wang, J., Schorter, L., Nguyen Trong, T. P., Fell, S., Ulrich, S., & Straubinger, R. K. (2020). Rapid clearance of Borrelia burgdorferi from the blood circulation. Parasites & Vectors, 13(1), Article 191. https://doi.org/10.1186/s13071-020-04060-y","havard":"L. Liang, J. Wang, L. Schorter, T.P. Nguyen Trong, S. Fell, S. Ulrich, R.K. Straubinger, Rapid clearance of Borrelia burgdorferi from the blood circulation, Parasites & Vectors. 13 (2020).","chicago-de":"Liang, Liucun, Jinyong Wang, Lucas Schorter, Thu Phong Nguyen Trong, Shari Fell, Sebastian Ulrich und Reinhard K. Straubinger. 2020. Rapid clearance of Borrelia burgdorferi from the blood circulation. Parasites & vectors 13, Nr. 1. doi:10.1186/s13071-020-04060-y, .","bjps":"Liang L et al. (2020) Rapid Clearance of Borrelia Burgdorferi from the Blood Circulation. Parasites & vectors 13.","ama":"Liang L, Wang J, Schorter L, et al. Rapid clearance of Borrelia burgdorferi from the blood circulation. Parasites & vectors. 2020;13(1). doi:10.1186/s13071-020-04060-y","van":"Liang L, Wang J, Schorter L, Nguyen Trong TP, Fell S, Ulrich S, et al. Rapid clearance of Borrelia burgdorferi from the blood circulation. Parasites & vectors. 2020;13(1).","short":"L. Liang, J. Wang, L. Schorter, T.P. Nguyen Trong, S. Fell, S. Ulrich, R.K. Straubinger, Parasites & Vectors 13 (2020).","ufg":"Liang, Liucun u. a.: Rapid clearance of Borrelia burgdorferi from the blood circulation, in: Parasites & vectors 13 (2020), H. 1.","din1505-2-1":"Liang, Liucun ; Wang, Jinyong ; Schorter, Lucas ; Nguyen Trong, Thu Phong ; Fell, Shari ; Ulrich, Sebastian ; Straubinger, Reinhard K.: Rapid clearance of Borrelia burgdorferi from the blood circulation. In: Parasites & vectors Bd. 13. London, BioMed Central (2020), Nr. 1"},"language":[{"iso":"eng"}],"publisher":"BioMed Central ","extern":"1","article_number":"191","status":"public","publication_identifier":{"issn":["1756-3305"]},"date_created":"2025-06-15T10:13:30Z","quality_controlled":"1","publication_status":"published","volume":13,"date_updated":"2025-06-17T14:17:27Z","department":[{"_id":"DEP4010"}],"abstract":[{"lang":"eng","text":"Background\r\nBorrelia burgdorferi is a tick-borne spirochete that causes Lyme borreliosis (LB). After an initial tick bite, it spreads from the deposition site in the dermis to distant tissues of the host. It is generally believed that this spirochete disseminates via the hematogenous route. Borrelia persica causes relapsing fever and is able to replicate in the blood stream. Currently the exact dissemination pathway of LB pathogens in the host is not known and controversially discussed.\r\nMethods\r\nIn this study, we established a strict intravenous infection murine model using host-adapted spirochetes. Survival capacity and infectivity of host-adapted B. burgdorferi sensu stricto (Bbss) were compared to those of B. persica (Bp) after either intradermal (ID) injection into the dorsal skin of immunocompetent mice or strict intravenous (IV) inoculation via the jugular vein. By in vitro culture and PCR, viable spirochetes and their DNA load in peripheral blood were periodically monitored during a 49/50-day course post-injection, as well as in various tissue samples collected at day 49/50. Specific antibodies in individual plasma/serum samples were detected with serological methods.\r\nResults\r\nRegardless of ID or IV injection, DNA of Bp was present in blood samples up to day 24 post-challenge, while no Bbss was detectable in the blood circulation during the complete observation period. In contrast to the brain tropism of Bp, Bbss spirochetes were found in ear, skin, joint, bladder, and heart tissue samples of only ID-inoculated mice. All tested tissues collected from IV-challenged mice were negative for traces of Bbss. ELISA testing of serum samples showed that Bp induced gradually increasing antibody levels after ID or IV inoculation, while Bbss did so only after ID injection but not after IV inoculation.\r\nConclusions\r\nThis study allows us to draw the following conclusions: (i) Bp survives in the blood and disseminates to the host’s brain via the hematogenous route; and (ii) Bbss, in contrast, is cleared rapidly from the blood stream and is a tissue-bound spirochete."}],"year":"2020","author":[{"last_name":"Liang","full_name":"Liang, Liucun","first_name":"Liucun"},{"last_name":"Wang","full_name":"Wang, Jinyong","first_name":"Jinyong"},{"first_name":"Lucas","full_name":"Schorter, Lucas","last_name":"Schorter"},{"first_name":"Thu Phong","last_name":"Nguyen Trong","full_name":"Nguyen Trong, Thu Phong"},{"last_name":"Fell","full_name":"Fell, Shari","first_name":"Shari"},{"full_name":"Ulrich, Sebastian","id":"85847","orcid":"0000-0002-4511-9537","last_name":"Ulrich","first_name":"Sebastian"},{"last_name":"Straubinger","full_name":"Straubinger, Reinhard K.","first_name":"Reinhard K."}],"title":"Rapid clearance of Borrelia burgdorferi from the blood circulation","issue":"1","type":"scientific_journal_article","place":"London"},{"status":"public","extern":"1","language":[{"iso":"eng"}],"citation":{"chicago":"Neumann-Cip, Anna-Cathrine, Volker Fingerle, Gabriele Margos, Reinhard K. Straubinger, Evelyn Overzier, Sebastian Ulrich, and Andreas Wieser. “A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate Differentiation.” Frontiers in Microbiology 11 (2020). https://doi.org/10.3389/fmicb.2020.00690.","mla":"Neumann-Cip, Anna-Cathrine, et al. “A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate Differentiation.” Frontiers in Microbiology, vol. 11, 2020, https://doi.org/10.3389/fmicb.2020.00690.","ieee":"A.-C. Neumann-Cip et al., “A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation,” Frontiers in Microbiology, vol. 11, 2020, doi: 10.3389/fmicb.2020.00690.","havard":"A.-C. Neumann-Cip, V. Fingerle, G. Margos, R.K. Straubinger, E. Overzier, S. Ulrich, A. Wieser, A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation, Frontiers in Microbiology. 11 (2020).","chicago-de":"Neumann-Cip, Anna-Cathrine, Volker Fingerle, Gabriele Margos, Reinhard K. Straubinger, Evelyn Overzier, Sebastian Ulrich und Andreas Wieser. 2020. A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology 11. doi:10.3389/fmicb.2020.00690, .","apa":"Neumann-Cip, A.-C., Fingerle, V., Margos, G., Straubinger, R. K., Overzier, E., Ulrich, S., & Wieser, A. (2020). A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.00690","bjps":"Neumann-Cip A-C et al. (2020) A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology 11.","ama":"Neumann-Cip AC, Fingerle V, Margos G, et al. A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology. 2020;11. doi:10.3389/fmicb.2020.00690","van":"Neumann-Cip AC, Fingerle V, Margos G, Straubinger RK, Overzier E, Ulrich S, et al. A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology. 2020;11.","ufg":"Neumann-Cip, Anna-Cathrine u. a.: A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation, in: Frontiers in Microbiology 11 (2020).","short":"A.-C. Neumann-Cip, V. Fingerle, G. Margos, R.K. Straubinger, E. Overzier, S. Ulrich, A. Wieser, Frontiers in Microbiology 11 (2020).","din1505-2-1":"Neumann-Cip, Anna-Cathrine ; Fingerle, Volker ; Margos, Gabriele ; Straubinger, Reinhard K. ; Overzier, Evelyn ; Ulrich, Sebastian ; Wieser, Andreas: A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation. In: Frontiers in Microbiology Bd. 11. Lausanne, Frontiers Media SA (2020)"},"publisher":"Frontiers Media SA","user_id":"83781","_id":"12963","keyword":["Borrelia burgdorferi sensu lato","MALDI-TOF MS","typing","sample preparation","MALDI-TOF MS library","strain typing","automatic identification"],"intvolume":" 11","publication":"Frontiers in Microbiology","doi":"10.3389/fmicb.2020.00690","place":"Lausanne","type":"scientific_journal_article","title":"A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation","author":[{"first_name":"Anna-Cathrine","last_name":"Neumann-Cip","full_name":"Neumann-Cip, Anna-Cathrine"},{"full_name":"Fingerle, Volker","last_name":"Fingerle","first_name":"Volker"},{"first_name":"Gabriele","full_name":"Margos, Gabriele","last_name":"Margos"},{"first_name":"Reinhard K.","full_name":"Straubinger, Reinhard K.","last_name":"Straubinger"},{"last_name":"Overzier","full_name":"Overzier, Evelyn","first_name":"Evelyn"},{"orcid":"0000-0002-4511-9537","last_name":"Ulrich","id":"85847","full_name":"Ulrich, Sebastian","first_name":"Sebastian"},{"first_name":"Andreas","last_name":"Wieser","full_name":"Wieser, Andreas"}],"abstract":[{"text":"The genus Borrelia comprises vector-borne bacterial pathogens that can severely affect human and animal health. Members of the Borrelia burgdorferi sensu lato species complex can cause Lyme borreliosis, one of the most common vector-borne diseases in the Northern hemisphere. Besides, members of the relapsing fever group of spirochetes can cause tick-borne relapsing fever in humans and various febrile illnesses in animals in tropical, subtropical and temperate regions. Borrelia spp. organisms are fastidious to cultivate and to maintain in vitro, and therefore, difficult to work with in the laboratory. Currently, borrelia identification is mainly performed using PCR and DNA sequencing methods, which can be complicated/frustrating on complex DNA templates and may still be relatively expensive. Alternative techniques such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) are not well established for Borrelia spp., although this technique is currently one of the most used techniques for rapid identification of bacteria in microbiological diagnostic laboratories. This is mainly due to unsatisfactory results obtained by use of simple sample preparation techniques and medium-contamination obscuring the mass spectra. In addition, comprehensive libraries for Borrelia spp. MALDI-TOF MS have yet to be established. In this study, we developed a new filter-based chemical extraction technique that allows measurement of high quality Borrelia spp. spectra from less than 100,000 bacteria per spot in MALDI-TOF MS. We used 49 isolates of 13 different species to produce the largest mass-library for Borrelia spp. so far and to validate the protocol. The library was successfully established and identifies >96% of used isolates correctly to species level. Cluster analysis on the sum spectra was applied to all the different isolates, which resulted in tight cluster generation for most species. Comparative analysis of the generated cluster to a phylogeny based on concatenated multi-locus sequence typing genes provided a surprising homology. Our data demonstrate that the technique described here can be used for fast and reliable species and strain typing within the borrelia complex.","lang":"eng"}],"year":"2020","department":[{"_id":"DEP4010"}],"publication_status":"published","volume":11,"date_updated":"2025-06-18T11:09:40Z","quality_controlled":"1","publication_identifier":{"eissn":["1664-302X"]},"date_created":"2025-06-15T10:15:09Z"},{"language":[{"iso":"eng"}],"citation":{"ama":"Ulrich S, Ebel F. Monoclonal Antibodies as Tools to Combat Fungal Infections. Journal of Fungi. 2020;6(1). doi:10.3390/jof6010022","mla":"Ulrich, Sebastian, and Frank Ebel. “Monoclonal Antibodies as Tools to Combat Fungal Infections.” Journal of Fungi, vol. 6, no. 1, 22, 2020, https://doi.org/10.3390/jof6010022.","van":"Ulrich S, Ebel F. Monoclonal Antibodies as Tools to Combat Fungal Infections. Journal of Fungi. 2020;6(1).","chicago":"Ulrich, Sebastian, and Frank Ebel. “Monoclonal Antibodies as Tools to Combat Fungal Infections.” Journal of Fungi 6, no. 1 (2020). https://doi.org/10.3390/jof6010022.","bjps":"Ulrich S and Ebel F (2020) Monoclonal Antibodies as Tools to Combat Fungal Infections. Journal of Fungi 6.","apa":"Ulrich, S., & Ebel, F. (2020). Monoclonal Antibodies as Tools to Combat Fungal Infections. Journal of Fungi, 6(1), Article 22. https://doi.org/10.3390/jof6010022","din1505-2-1":"Ulrich, Sebastian ; Ebel, Frank: Monoclonal Antibodies as Tools to Combat Fungal Infections. In: Journal of Fungi Bd. 6. Basel, MDPI (2020), Nr. 1","chicago-de":"Ulrich, Sebastian und Frank Ebel. 2020. Monoclonal Antibodies as Tools to Combat Fungal Infections. Journal of Fungi 6, Nr. 1. doi:10.3390/jof6010022, .","havard":"S. Ulrich, F. Ebel, Monoclonal Antibodies as Tools to Combat Fungal Infections, Journal of Fungi. 6 (2020).","short":"S. Ulrich, F. Ebel, Journal of Fungi 6 (2020).","ufg":"Ulrich, Sebastian/Ebel, Frank: Monoclonal Antibodies as Tools to Combat Fungal Infections, in: Journal of Fungi 6 (2020), H. 1.","ieee":"S. Ulrich and F. Ebel, “Monoclonal Antibodies as Tools to Combat Fungal Infections,” Journal of Fungi, vol. 6, no. 1, Art. no. 22, 2020, doi: 10.3390/jof6010022."},"publisher":"MDPI ","keyword":["monoclonal antibodies","invasive fungal infections","therapy","prophylaxis","opsonization"],"_id":"12964","user_id":"83781","intvolume":" 6","doi":"10.3390/jof6010022","publication":"Journal of Fungi","article_number":"22","status":"public","extern":"1","volume":6,"publication_status":"published","date_updated":"2025-06-18T11:13:40Z","quality_controlled":"1","publication_identifier":{"eissn":["2309-608X"]},"date_created":"2025-06-15T10:17:00Z","place":"Basel","type":"scientific_journal_article","title":"Monoclonal Antibodies as Tools to Combat Fungal Infections","issue":"1","author":[{"first_name":"Sebastian","orcid":"0000-0002-4511-9537","last_name":"Ulrich","full_name":"Ulrich, Sebastian","id":"85847"},{"full_name":"Ebel, Frank","last_name":"Ebel","first_name":"Frank"}],"abstract":[{"text":"Antibodies represent an important element in the adaptive immune response and a major tool to eliminate microbial pathogens. For many bacterial and viral infections, efficient vaccines exist, but not for fungal pathogens. For a long time, antibodies have been assumed to be of minor importance for a successful clearance of fungal infections; however this perception has been challenged by a large number of studies over the last three decades. In this review, we focus on the potential therapeutic and prophylactic use of monoclonal antibodies. Since systemic mycoses normally occur in severely immunocompromised patients, a passive immunization using monoclonal antibodies is a promising approach to directly attack the fungal pathogen and/or to activate and strengthen the residual antifungal immune response in these patients.","lang":"eng"}],"year":"2020","department":[{"_id":"DEP4010"}]},{"date_updated":"2025-06-18T11:16:35Z","publication_status":"published","volume":82,"quality_controlled":"1","date_created":"2025-06-15T10:18:20Z","publication_identifier":{"issn":["0740-0020"],"eissn":["1095-9998"]},"type":"scientific_journal_article","place":"London","author":[{"orcid":"0000-0002-4511-9537","last_name":"Ulrich","full_name":"Ulrich, Sebastian","id":"85847","first_name":"Sebastian"},{"last_name":"Gottschalk","full_name":"Gottschalk, Christoph","first_name":"Christoph"},{"full_name":"Dietrich, Richard","last_name":"Dietrich","first_name":"Richard"},{"first_name":"Erwin","full_name":"Märtlbauer, Erwin","last_name":"Märtlbauer"},{"first_name":"Manfred","full_name":"Gareis, Manfred","last_name":"Gareis"}],"title":"Identification of cereulide producing Bacillus cereus by MALDI-TOF MS","year":"2019","abstract":[{"lang":"eng","text":"The Bacillus (B.) cereus group consists of nine recognized species which are present worldwide. B. cereus play an important role in food-borne diseases by producing different toxins. Yet, only a small percentage of B. cereus strains are able to produce the heat stable cereulide, the causative agent of emetic food poisoning. To minimize the entry of emetic B. cereus into the food chain, food business operators are dependent on efficient and reliable methods enabling the differentiation between emetic and non-emetic strains.\r\nCurrently, only time-consuming cell bioassays, molecular methods and tandem mass spectrometry are available for this purpose. Thus, the aim of the present study was to establish a fast and reliable method for the differentiation between emetic/non-emetic strains by MALDI-TOF MS. Selected strains/isolates of the B. cereus group as well as other Bacillus spp. (total n = 121) were cultured on sheep blood agar for 48 h before analysis.\r\nSubsequently, the cultures were directly analyzed by MALDI-TOF MS without prior extraction steps. The samples were measured in the mass range of m/z 800–1800 Da. Using ClinProTools 3.0 statistical software and Flex analysis software (Bruker Daltonics GmbH, Bremen, Germany), a differentiation between emetic/non-emetic isolates was possible with a rate of correct identification of 99.1% by means of the evaluation of two specific biomarkers (m/z 1171 and 1187 Da)."}],"department":[{"_id":"DEP4010"}],"publisher":"Academic Press ","citation":{"chicago":"Ulrich, Sebastian, Christoph Gottschalk, Richard Dietrich, Erwin Märtlbauer, and Manfred Gareis. “Identification of Cereulide Producing Bacillus Cereus by MALDI-TOF MS.” Food Microbiology 82 (2019): 75–81. https://doi.org/10.1016/j.fm.2019.01.012.","mla":"Ulrich, Sebastian, et al. “Identification of Cereulide Producing Bacillus Cereus by MALDI-TOF MS.” Food Microbiology, vol. 82, 2019, pp. 75–81, https://doi.org/10.1016/j.fm.2019.01.012.","ama":"Ulrich S, Gottschalk C, Dietrich R, Märtlbauer E, Gareis M. Identification of cereulide producing Bacillus cereus by MALDI-TOF MS. Food Microbiology. 2019;82:75-81. doi:10.1016/j.fm.2019.01.012","van":"Ulrich S, Gottschalk C, Dietrich R, Märtlbauer E, Gareis M. Identification of cereulide producing Bacillus cereus by MALDI-TOF MS. Food Microbiology. 2019;82:75–81.","bjps":"Ulrich S et al. (2019) Identification of Cereulide Producing Bacillus Cereus by MALDI-TOF MS. Food Microbiology 82, 75–81.","din1505-2-1":"Ulrich, Sebastian ; Gottschalk, Christoph ; Dietrich, Richard ; Märtlbauer, Erwin ; Gareis, Manfred: Identification of cereulide producing Bacillus cereus by MALDI-TOF MS. In: Food Microbiology Bd. 82. London, Academic Press (2019), S. 75–81","chicago-de":"Ulrich, Sebastian, Christoph Gottschalk, Richard Dietrich, Erwin Märtlbauer und Manfred Gareis. 2019. Identification of cereulide producing Bacillus cereus by MALDI-TOF MS. Food Microbiology 82: 75–81. doi:10.1016/j.fm.2019.01.012, .","havard":"S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, M. Gareis, Identification of cereulide producing Bacillus cereus by MALDI-TOF MS, Food Microbiology. 82 (2019) 75–81.","apa":"Ulrich, S., Gottschalk, C., Dietrich, R., Märtlbauer, E., & Gareis, M. (2019). Identification of cereulide producing Bacillus cereus by MALDI-TOF MS. Food Microbiology, 82, 75–81. https://doi.org/10.1016/j.fm.2019.01.012","ieee":"S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, and M. Gareis, “Identification of cereulide producing Bacillus cereus by MALDI-TOF MS,” Food Microbiology, vol. 82, pp. 75–81, 2019, doi: 10.1016/j.fm.2019.01.012.","ufg":"Ulrich, Sebastian u. a.: Identification of cereulide producing Bacillus cereus by MALDI-TOF MS, in: Food Microbiology 82 (2019), S. 75–81.","short":"S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, M. Gareis, Food Microbiology 82 (2019) 75–81."},"language":[{"iso":"eng"}],"page":"75-81","user_id":"83781","_id":"12965","keyword":["MALDI-TOF MS","Bacillus cereus","Cereulide","Food intoxication"],"doi":"10.1016/j.fm.2019.01.012","publication":"Food Microbiology","intvolume":" 82","status":"public","extern":"1"},{"date_updated":"2025-06-18T11:20:11Z","volume":36,"publication_status":"published","date_created":"2025-06-15T10:19:53Z","publication_identifier":{"issn":["0178-7888"],"eissn":["1867-1632"]},"quality_controlled":"1","issue":"1","title":"Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes","author":[{"first_name":"Sebastian","last_name":"Ulrich","orcid":"0000-0002-4511-9537","id":"85847","full_name":"Ulrich, Sebastian"},{"first_name":"Ludwig","last_name":"Niessen","full_name":"Niessen, Ludwig"},{"last_name":"Ekruth","full_name":"Ekruth, Julia","first_name":"Julia"},{"last_name":"Schäfer","full_name":"Schäfer, Cornelius","first_name":"Cornelius"},{"last_name":"Kaltner","full_name":"Kaltner, Florian","first_name":"Florian"},{"last_name":"Gottschalk","full_name":"Gottschalk, Christoph","first_name":"Christoph"}],"place":"Berlin ; Heidelberg","type":"scientific_journal_article","department":[{"_id":"DEP4010"}],"year":"2019","abstract":[{"lang":"eng","text":"The fungus Stachybotrys (S.) chartarum was isolated from culinary herbs, damp building materials, and improperly stored animal forage. Two distinct chemotypes of the fungus were described that produced either high-cytotoxic macrocyclic trichothecenes (S type) or low-cytotoxic atranones (A type). Recently, two distinct gene clusters were described that were found to be necessary for the biosynthesis of either macrocyclic trichothecenes (21 SAT (Satratoxin) genes) or atranones (14 ATR (Atranone) genes). In the current study, PCR primers were designed to detect SAT and ATR genes in 19 S. chartarum chemotype S and eight S. chartarum chemotype A strains. Our analysis revealed the existence of three different genotypes: satratoxin-producing strains that harbored all SAT genes but lacked the ATR gene cluster (genotype S), non-satratoxin-producing strains that possessed the ATR genes but lacked SAT genes (genotype A), and a hitherto undescribed hybrid genotype among non-satratoxin-producing strains that harbored all ATR genes and an incomplete set of SAT genes (genotype H). In order to improve the discrimination of genotypes, a triplex PCR assay was developed and applied for the analysis of S. chartarum and S. chlorohalonata cultures. The results show that genes for macrocyclic trichothecenes and atranones are not mutually exclusive in S. chartarum. Correlation of the new genotype-based concept with mycotoxin production data shows also that macrocyclic trichothecenes are exclusively produced by S. chartarum genotype S strains."}],"page":"83-91","keyword":["Acetyltransferases","Chlamydomonas reinhardtii","Fungal Genes","Fungal genetics","Fungal genomics","Saccharomyces cerevisiae"],"_id":"12966","user_id":"83781","publisher":"Springer","language":[{"iso":"eng"}],"citation":{"ufg":"Ulrich, Sebastian u. a.: Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes, in: Mycotoxin Research 36 (2019), H. 1, S. 83–91.","short":"S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, C. Gottschalk, Mycotoxin Research 36 (2019) 83–91.","din1505-2-1":"Ulrich, Sebastian ; Niessen, Ludwig ; Ekruth, Julia ; Schäfer, Cornelius ; Kaltner, Florian ; Gottschalk, Christoph: Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. In: Mycotoxin Research Bd. 36. Berlin ; Heidelberg, Springer (2019), Nr. 1, S. 83–91","bjps":"Ulrich S et al. (2019) Truncated Satratoxin Gene Clusters in Selected Isolates of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.) S. Hughes. Mycotoxin Research 36, 83–91.","van":"Ulrich S, Niessen L, Ekruth J, Schäfer C, Kaltner F, Gottschalk C. Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. Mycotoxin Research. 2019;36(1):83–91.","ama":"Ulrich S, Niessen L, Ekruth J, Schäfer C, Kaltner F, Gottschalk C. Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. Mycotoxin Research. 2019;36(1):83-91. doi:10.1007/s12550-019-00371-x","ieee":"S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, and C. Gottschalk, “Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes,” Mycotoxin Research, vol. 36, no. 1, pp. 83–91, 2019, doi: 10.1007/s12550-019-00371-x.","chicago-de":"Ulrich, Sebastian, Ludwig Niessen, Julia Ekruth, Cornelius Schäfer, Florian Kaltner und Christoph Gottschalk. 2019. Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. Mycotoxin Research 36, Nr. 1: 83–91. doi:10.1007/s12550-019-00371-x, .","havard":"S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, C. Gottschalk, Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes, Mycotoxin Research. 36 (2019) 83–91.","apa":"Ulrich, S., Niessen, L., Ekruth, J., Schäfer, C., Kaltner, F., & Gottschalk, C. (2019). Truncated satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. Mycotoxin Research, 36(1), 83–91. https://doi.org/10.1007/s12550-019-00371-x","chicago":"Ulrich, Sebastian, Ludwig Niessen, Julia Ekruth, Cornelius Schäfer, Florian Kaltner, and Christoph Gottschalk. “Truncated Satratoxin Gene Clusters in Selected Isolates of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.) S. Hughes.” Mycotoxin Research 36, no. 1 (2019): 83–91. https://doi.org/10.1007/s12550-019-00371-x.","mla":"Ulrich, Sebastian, et al. “Truncated Satratoxin Gene Clusters in Selected Isolates of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.) S. Hughes.” Mycotoxin Research, vol. 36, no. 1, 2019, pp. 83–91, https://doi.org/10.1007/s12550-019-00371-x."},"intvolume":" 36","publication":"Mycotoxin Research","doi":"10.1007/s12550-019-00371-x","status":"public","extern":"1"},{"date_created":"2025-06-15T10:20:55Z","publication_identifier":{"issn":["0022-4510","1748-5827"]},"quality_controlled":"1","date_updated":"2025-06-18T11:24:12Z","publication_status":"published","volume":61,"department":[{"_id":"DEP4010"}],"year":"2019","abstract":[{"lang":"eng","text":"Objectives\r\nTo evaluate matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF MS) combined with the Sepsityper kit (Bruker Daltoniks GmbH, Bremen) for the direct detection of bacterial species from inoculated blood cultures from dogs and cats.\r\nMaterials and Methods\r\nCanine and feline blood samples were inoculated with typical sepsis-causing bacteria such as Staphylococcus intermedius, Staphylococcus aureus, Streptococcus canis, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa at two distinct concentrations (each in triplicate), resulting in 72 blood culture bottles incubated at 37°C. Samples were comparatively analysed with MALDI-TOF MS after preparation with the Sepsityper kit and also by standard bacteriology (culturing and biochemical characterisation).\r\nResults\r\nBacterial species identified from agar plates and by MALDI-TOF MS from blood culture bottles were identical for all samples. The MALDI Biotyper software (Bruker Daltoniks) correctly identified all bacterial strains from inoculated canine and feline blood with analysis indicating very good precision.\r\nClinical Significance\r\nMALDI-TOF MS analysis combined with the Sepsityper kit is a reliable tool for a quick detection of veterinary-relevant bacterial species directly from blood culture bottles. This approach could reduce the time for identification of critical species to only 24 hours."}],"title":"Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood","issue":"1","author":[{"orcid":"0000-0002-4511-9537","last_name":"Ulrich","id":"85847","full_name":"Ulrich, Sebastian","first_name":"Sebastian"},{"first_name":"C.","last_name":"Gottschalk","full_name":"Gottschalk, C."},{"full_name":"Straubinger, R. Kk","last_name":"Straubinger","first_name":"R. Kk"},{"last_name":"Schwaiger","full_name":"Schwaiger, K.","first_name":"K."},{"first_name":"R.","full_name":"Dörfelt, R.","last_name":"Dörfelt"}],"place":"Oxford [u.a.]","type":"scientific_journal_article","intvolume":" 61","publication":"Journal of Small Animal Practice","doi":"10.1111/jsap.13056","page":"42-45","_id":"12967","user_id":"83781","publisher":"Wiley-Blackwell","language":[{"iso":"eng"}],"citation":{"din1505-2-1":"Ulrich, Sebastian ; Gottschalk, C. ; Straubinger, R. Kk ; Schwaiger, K. ; Dörfelt, R.: Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood. In: Journal of Small Animal Practice Bd. 61. Oxford [u.a.], Wiley-Blackwell (2019), Nr. 1, S. 42–45","havard":"S. Ulrich, C. Gottschalk, R.K. Straubinger, K. Schwaiger, R. Dörfelt, Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood, Journal of Small Animal Practice. 61 (2019) 42–45.","chicago-de":"Ulrich, Sebastian, C. Gottschalk, R. Kk Straubinger, K. Schwaiger und R. Dörfelt. 2019. Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood. Journal of Small Animal Practice 61, Nr. 1: 42–45. doi:10.1111/jsap.13056, .","apa":"Ulrich, S., Gottschalk, C., Straubinger, R. K., Schwaiger, K., & Dörfelt, R. (2019). Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood. Journal of Small Animal Practice, 61(1), 42–45. https://doi.org/10.1111/jsap.13056","ieee":"S. Ulrich, C. Gottschalk, R. K. Straubinger, K. Schwaiger, and R. Dörfelt, “Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood,” Journal of Small Animal Practice, vol. 61, no. 1, pp. 42–45, 2019, doi: 10.1111/jsap.13056.","ufg":"Ulrich, Sebastian u. a.: Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood, in: Journal of Small Animal Practice 61 (2019), H. 1, S. 42–45.","short":"S. Ulrich, C. Gottschalk, R.K. Straubinger, K. Schwaiger, R. Dörfelt, Journal of Small Animal Practice 61 (2019) 42–45.","chicago":"Ulrich, Sebastian, C. Gottschalk, R. Kk Straubinger, K. Schwaiger, and R. Dörfelt. “Acceleration of the Identification of Sepsis‐inducing Bacteria in Cultures of Dog and Cat Blood.” Journal of Small Animal Practice 61, no. 1 (2019): 42–45. https://doi.org/10.1111/jsap.13056.","mla":"Ulrich, Sebastian, et al. “Acceleration of the Identification of Sepsis‐inducing Bacteria in Cultures of Dog and Cat Blood.” Journal of Small Animal Practice, vol. 61, no. 1, 2019, pp. 42–45, https://doi.org/10.1111/jsap.13056.","ama":"Ulrich S, Gottschalk C, Straubinger RK, Schwaiger K, Dörfelt R. Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood. Journal of Small Animal Practice. 2019;61(1):42-45. doi:10.1111/jsap.13056","van":"Ulrich S, Gottschalk C, Straubinger RK, Schwaiger K, Dörfelt R. Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood. Journal of Small Animal Practice. 2019;61(1):42–5.","bjps":"Ulrich S et al. (2019) Acceleration of the Identification of Sepsis‐inducing Bacteria in Cultures of Dog and Cat Blood. Journal of Small Animal Practice 61, 42–45."},"extern":"1","status":"public"},{"abstract":[{"lang":"eng","text":"The genus Stachybotrys produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct Stachybotrys species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different Stachybotrys strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of Stachybotrys species are considerably influenced by time of growth and substrate availability, as well as the individual biosynthetic potential of the respective species. Regarding the reported adverse effects associated with Stachybotrys growth in building environments, combinatory effects of the investigated secondary metabolites should be addressed and the role of the phenylspirodrimanes re-evaluated in future research."}],"year":"2019","department":[{"_id":"DEP4010"}],"place":"Basel","type":"scientific_journal_article","title":"Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS","issue":"3","author":[{"first_name":"Annika","last_name":"Jagels","full_name":"Jagels, Annika"},{"last_name":"Lindemann","full_name":"Lindemann, Viktoria","first_name":"Viktoria"},{"full_name":"Ulrich, Sebastian","id":"85847","orcid":"0000-0002-4511-9537","last_name":"Ulrich","first_name":"Sebastian"},{"full_name":"Gottschalk, Christoph","last_name":"Gottschalk","first_name":"Christoph"},{"full_name":"Cramer, Benedikt","last_name":"Cramer","first_name":"Benedikt"},{"last_name":"Hübner","full_name":"Hübner, Florian","first_name":"Florian"},{"first_name":"Manfred","last_name":"Gareis","full_name":"Gareis, Manfred"},{"first_name":"Hans-Ulrich","last_name":"Humpf","full_name":"Humpf, Hans-Ulrich"}],"quality_controlled":"1","publication_identifier":{"eissn":["2072-6651"]},"date_created":"2025-06-15T10:23:36Z","publication_status":"published","volume":11,"date_updated":"2025-06-17T14:12:55Z","extern":"1","article_number":"133","status":"public","intvolume":" 11","doi":"10.3390/toxins11030133","publication":"Toxins","language":[{"iso":"eng"}],"citation":{"havard":"A. Jagels, V. Lindemann, S. Ulrich, C. Gottschalk, B. Cramer, F. Hübner, M. Gareis, H.-U. Humpf, Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS, Toxins. 11 (2019).","chicago-de":"Jagels, Annika, Viktoria Lindemann, Sebastian Ulrich, Christoph Gottschalk, Benedikt Cramer, Florian Hübner, Manfred Gareis und Hans-Ulrich Humpf. 2019. Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. Toxins 11, Nr. 3. doi:10.3390/toxins11030133, .","apa":"Jagels, A., Lindemann, V., Ulrich, S., Gottschalk, C., Cramer, B., Hübner, F., Gareis, M., & Humpf, H.-U. (2019). Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. Toxins, 11(3), Article 133. https://doi.org/10.3390/toxins11030133","ieee":"A. Jagels et al., “Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS,” Toxins, vol. 11, no. 3, Art. no. 133, 2019, doi: 10.3390/toxins11030133.","chicago":"Jagels, Annika, Viktoria Lindemann, Sebastian Ulrich, Christoph Gottschalk, Benedikt Cramer, Florian Hübner, Manfred Gareis, and Hans-Ulrich Humpf. “Exploring Secondary Metabolite Profiles of Stachybotrys Spp. by LC-MS/MS.” Toxins 11, no. 3 (2019). https://doi.org/10.3390/toxins11030133.","mla":"Jagels, Annika, et al. “Exploring Secondary Metabolite Profiles of Stachybotrys Spp. by LC-MS/MS.” Toxins, vol. 11, no. 3, 133, 2019, https://doi.org/10.3390/toxins11030133.","din1505-2-1":"Jagels, Annika ; Lindemann, Viktoria ; Ulrich, Sebastian ; Gottschalk, Christoph ; Cramer, Benedikt ; Hübner, Florian ; Gareis, Manfred ; Humpf, Hans-Ulrich: Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. In: Toxins Bd. 11. Basel, MDPI (2019), Nr. 3","ufg":"Jagels, Annika u. a.: Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS, in: Toxins 11 (2019), H. 3.","short":"A. Jagels, V. Lindemann, S. Ulrich, C. Gottschalk, B. Cramer, F. Hübner, M. Gareis, H.-U. Humpf, Toxins 11 (2019).","ama":"Jagels A, Lindemann V, Ulrich S, et al. Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. Toxins. 2019;11(3). doi:10.3390/toxins11030133","van":"Jagels A, Lindemann V, Ulrich S, Gottschalk C, Cramer B, Hübner F, et al. Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. Toxins. 2019;11(3).","bjps":"Jagels A et al. (2019) Exploring Secondary Metabolite Profiles of Stachybotrys Spp. by LC-MS/MS. Toxins 11."},"publisher":"MDPI","_id":"12971","user_id":"83781","keyword":["Stachybotrys spp.","metabolite profiles","LC-MS/MS","satratoxins","phenylspirodrimanes","stachybotrychromenes","biosynthetic production"]},{"author":[{"first_name":"Samart","last_name":"Dorn-In","full_name":"Dorn-In, Samart"},{"first_name":"Karin","full_name":"Schwaiger, Karin","last_name":"Schwaiger"},{"full_name":"Springer, Claudia","last_name":"Springer","first_name":"Claudia"},{"full_name":"Barta, Leonard","last_name":"Barta","first_name":"Leonard"},{"id":"85847","full_name":"Ulrich, Sebastian","last_name":"Ulrich","orcid":"0000-0002-4511-9537","first_name":"Sebastian"},{"last_name":"Gareis","full_name":"Gareis, Manfred","first_name":"Manfred"}],"title":"Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars","type":"scientific_journal_article","place":"Amsterdam","department":[{"_id":"DEP4010"}],"abstract":[{"lang":"eng","text":"Psychrophilic and psychrotolerant clostridia (n = 110) were isolated from vacuum-packed meat (beef and lamb), fresh venison and from skin and fecal samples of wild boars. They were identified to species level using MALDI-TOF MS, sequence and phylogeny analysis of the 16S rRNA and species specific multiplex qPCR. The results of all three methods were concordant. The majority of isolates were identified as C. tagluense-like Group I (n = 34) and Group II (n = 42). Thirty-five isolates could be identified to species level as follows: C. estertheticum (n = 15), C. frigoriphilum (n = 13), C. frigidicarnis (n = 1) and C. bowmanii (n = 5). This is the first report of detection and identification of C. frigoriphilum and C. tagluense-like Group II as causative agents of blown pack spoilage of beef. The species specific multiplex qPCR developed in this study could be applied to identify and to quantify the Clostridium species described above in suspicious meat juice samples."}],"year":"2018","publication_status":"published","volume":286,"date_updated":"2025-06-18T11:27:12Z","publication_identifier":{"issn":["0168-1605"],"eissn":["1879-3460"]},"date_created":"2025-06-15T10:25:24Z","quality_controlled":"1","status":"public","extern":"1","keyword":["Blown pack spoilage","MALDI-TOF MS","PCR","16S rRNA","C. frigoriphilum","C. tagluense-like"],"_id":"12973","user_id":"83781","page":"162-169","citation":{"short":"S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, M. Gareis, International Journal of Food Microbiology 286 (2018) 162–169.","ufg":"Dorn-In, Samart u. a.: Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars, in: International Journal of Food Microbiology 286 (2018), S. 162–169.","din1505-2-1":"Dorn-In, Samart ; Schwaiger, Karin ; Springer, Claudia ; Barta, Leonard ; Ulrich, Sebastian ; Gareis, Manfred: Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars. In: International Journal of Food Microbiology Bd. 286. Amsterdam, Elsevier (2018), S. 162–169","bjps":"Dorn-In S et al. (2018) Development of a Multiplex QPCR for the Species Identification of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like from Blown Pack Spoilage (BPS) Meats and from Wild Boars. International Journal of Food Microbiology 286, 162–169.","ama":"Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars. International Journal of Food Microbiology. 2018;286:162-169. doi:10.1016/j.ijfoodmicro.2018.08.020","van":"Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars. International Journal of Food Microbiology. 2018;286:162–9.","ieee":"S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, and M. Gareis, “Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars,” International Journal of Food Microbiology, vol. 286, pp. 162–169, 2018, doi: 10.1016/j.ijfoodmicro.2018.08.020.","apa":"Dorn-In, S., Schwaiger, K., Springer, C., Barta, L., Ulrich, S., & Gareis, M. (2018). Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars. International Journal of Food Microbiology, 286, 162–169. https://doi.org/10.1016/j.ijfoodmicro.2018.08.020","havard":"S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, M. Gareis, Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars, International Journal of Food Microbiology. 286 (2018) 162–169.","chicago-de":"Dorn-In, Samart, Karin Schwaiger, Claudia Springer, Leonard Barta, Sebastian Ulrich und Manfred Gareis. 2018. Development of a multiplex qPCR for the species identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars. International Journal of Food Microbiology 286: 162–169. doi:10.1016/j.ijfoodmicro.2018.08.020, .","mla":"Dorn-In, Samart, et al. “Development of a Multiplex QPCR for the Species Identification of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like from Blown Pack Spoilage (BPS) Meats and from Wild Boars.” International Journal of Food Microbiology, vol. 286, 2018, pp. 162–69, https://doi.org/10.1016/j.ijfoodmicro.2018.08.020.","chicago":"Dorn-In, Samart, Karin Schwaiger, Claudia Springer, Leonard Barta, Sebastian Ulrich, and Manfred Gareis. “Development of a Multiplex QPCR for the Species Identification of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like from Blown Pack Spoilage (BPS) Meats and from Wild Boars.” International Journal of Food Microbiology 286 (2018): 162–69. https://doi.org/10.1016/j.ijfoodmicro.2018.08.020."},"language":[{"iso":"eng"}],"publisher":"Elsevier ","publication":"International Journal of Food Microbiology","doi":"10.1016/j.ijfoodmicro.2018.08.020","intvolume":" 286"},{"quality_controlled":"1","publication_identifier":{"issn":["0022-5142"],"eissn":["1097-0010"]},"date_created":"2025-06-15T10:28:46Z","volume":98,"publication_status":"published","date_updated":"2025-06-18T11:32:30Z","abstract":[{"lang":"eng","text":"BACKGROUND\r\nFruits and vegetables have increasingly been related to foodborne outbreaks. Besides surface contamination, a possible internalization of microorganisms into edible parts of plants during growth has already been observed. To examine an actual risk for the consumer, microbial contamination of the rind and pulp of 147 muskmelons from international trade was assessed using cultural and biochemical methods, polymerase chain reaction and matrix-assisted laser desorption/ionization-time of flight mass spectrometry.\r\nRESULTS\r\nOne hundred percent of the rind samples [3.69–8.92 log colony forming units (CFU) g−1] and 89.8% of the pulp samples (maximum load 3.66 log CFU g−1) were microbiologically contaminated. Among the 432 pulp isolates, opportunistic and potentially pathogenic bacteria were identified, mainly Staphylococcus spp. (48.9%), Clostridium spp. (42.9%) and Enterobacteriaceae (27.9%). Salmonella spp., Escherichia coli and isolates of the Bacillus cereus group were found on the rind (1.4%, 0.7% and 42.9%, respectively) and in the pulp (0.7%, 1.4% and 4.7%). Clostridium perfringens was isolated from the rind of seven melons.\r\nCONCLUSION\r\nThe present study revealed a regularly occurring internal contamination of melons. Possible health risks for consumers because of an occurrence of microorganisms in melon pulp should be considered in future food safety assessments. © 2018 Society of Chemical Industry."}],"year":"2018","department":[{"_id":"DEP4010"}],"place":"Chichester","type":"scientific_journal_article","title":"Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer?","issue":"13","author":[{"last_name":"Esteban‐Cuesta","full_name":"Esteban‐Cuesta, Irene","first_name":"Irene"},{"full_name":"Drees, Nathalie","last_name":"Drees","first_name":"Nathalie"},{"first_name":"Sebastian","last_name":"Ulrich","orcid":"0000-0002-4511-9537","full_name":"Ulrich, Sebastian","id":"85847"},{"first_name":"Peter","last_name":"Stauch","full_name":"Stauch, Peter"},{"first_name":"Brigitte","full_name":"Sperner, Brigitte","last_name":"Sperner"},{"full_name":"Schwaiger, Karin","last_name":"Schwaiger","first_name":"Karin"},{"first_name":"Manfred","full_name":"Gareis, Manfred","last_name":"Gareis"},{"first_name":"Christoph","last_name":"Gottschalk","full_name":"Gottschalk, Christoph"}],"intvolume":" 98","publication":"Journal of the science of food and agriculture : incorporating Agri-Biotech","doi":"10.1002/jsfa.9045","language":[{"iso":"eng"}],"citation":{"mla":"Esteban‐Cuesta, Irene, et al. “Endogenous Microbial Contamination of Melons (Cucumis Melo) from International Trade: An Underestimated Risk for the Consumer?” Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech, vol. 98, no. 13, 2018, pp. 5074–81, https://doi.org/10.1002/jsfa.9045.","chicago":"Esteban‐Cuesta, Irene, Nathalie Drees, Sebastian Ulrich, Peter Stauch, Brigitte Sperner, Karin Schwaiger, Manfred Gareis, and Christoph Gottschalk. “Endogenous Microbial Contamination of Melons (Cucumis Melo) from International Trade: An Underestimated Risk for the Consumer?” Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech 98, no. 13 (2018): 5074–81. https://doi.org/10.1002/jsfa.9045.","ieee":"I. Esteban‐Cuesta et al., “Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer?,” Journal of the science of food and agriculture : incorporating Agri-Biotech, vol. 98, no. 13, pp. 5074–5081, 2018, doi: 10.1002/jsfa.9045.","apa":"Esteban‐Cuesta, I., Drees, N., Ulrich, S., Stauch, P., Sperner, B., Schwaiger, K., Gareis, M., & Gottschalk, C. (2018). Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer? Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech, 98(13), 5074–5081. https://doi.org/10.1002/jsfa.9045","chicago-de":"Esteban‐Cuesta, Irene, Nathalie Drees, Sebastian Ulrich, Peter Stauch, Brigitte Sperner, Karin Schwaiger, Manfred Gareis und Christoph Gottschalk. 2018. Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer? Journal of the science of food and agriculture : incorporating Agri-Biotech 98, Nr. 13: 5074–5081. doi:10.1002/jsfa.9045, .","havard":"I. Esteban‐Cuesta, N. Drees, S. Ulrich, P. Stauch, B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer?, Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech. 98 (2018) 5074–5081.","bjps":"Esteban‐Cuesta I et al. (2018) Endogenous Microbial Contamination of Melons (Cucumis Melo) from International Trade: An Underestimated Risk for the Consumer? Journal of the science of food and agriculture : incorporating Agri-Biotech 98, 5074–5081.","van":"Esteban‐Cuesta I, Drees N, Ulrich S, Stauch P, Sperner B, Schwaiger K, et al. Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer? Journal of the science of food and agriculture : incorporating Agri-Biotech. 2018;98(13):5074–81.","ama":"Esteban‐Cuesta I, Drees N, Ulrich S, et al. Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer? Journal of the science of food and agriculture : incorporating Agri-Biotech. 2018;98(13):5074-5081. doi:10.1002/jsfa.9045","short":"I. Esteban‐Cuesta, N. Drees, S. Ulrich, P. Stauch, B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech 98 (2018) 5074–5081.","ufg":"Esteban‐Cuesta, Irene u. a.: Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer?, in: Journal of the science of food and agriculture : incorporating Agri-Biotech 98 (2018), H. 13, S. 5074–5081.","din1505-2-1":"Esteban‐Cuesta, Irene ; Drees, Nathalie ; Ulrich, Sebastian ; Stauch, Peter ; Sperner, Brigitte ; Schwaiger, Karin ; Gareis, Manfred ; Gottschalk, Christoph: Endogenous microbial contamination of melons (Cucumis melo) from international trade: an underestimated risk for the consumer? In: Journal of the science of food and agriculture : incorporating Agri-Biotech Bd. 98. Chichester, Wiley (2018), Nr. 13, S. 5074–5081"},"publisher":"Wiley","_id":"12974","user_id":"83781","keyword":["foodborne pathogens","Salmonella","Listeria monocytogenes","Enterobacteriaceae","vegetables"],"page":"5074-5081","extern":"1","status":"public"},{"publication_identifier":{"eissn":["1537-2995"],"issn":["0041-1132"]},"date_created":"2025-06-17T06:55:03Z","quality_controlled":"1","volume":58,"publication_status":"published","date_updated":"2025-06-17T13:48:11Z","department":[{"_id":"DEP4010"}],"abstract":[{"lang":"eng","text":"BACKGROUND\r\nBacterial contamination of platelet concentrates (PCs) is still a major challenge in transfusion medicine. Different methodologic concepts and screening strategies have been developed and investigated concerning their usability. We evaluated the feasibility of BacT/ALERT automated culture (BacT/A, bioMérieux) with late sampling after 3 days at the earliest.\r\nSTUDY DESIGN AND METHODS\r\nTwenty-four bacterial strains isolated from PCs and six relevant strains from reference stocks were spiked into apheresis-derived PCs (10-60 colony-forming units [CFU]/bag). Sampling was performed after 3 days, and bacterial detection was investigated using the two detection methods (BacT/A and BactiFlow [BF], bioMérieux). The maximum time-to-result of BacT/A was set to less than 12 hours.\r\nRESULTS\r\nAll medium- or high-pathogenic strains are capable of proliferating to high titers, and 100% of contaminated samples were detected by BF and BacT/A (6 to ≤12 h incubation); lower detection rates of BacT/A were obtained within 6 hours of incubation (≤6 h: 76.2-93.4%). The majority of low-pathogenic isolates are also capable of growing in PCs (89.7%), showing a detection rate of 74.3% for BF versus 54.3% for BacT/A (6 to ≤12 h incubation). BacT/A failed to detect bacteria within 6 hours of incubation. Certainly, a small number of strains did not grow under PC storage conditions and were detectable by BacT/A only with increased detection times.\r\nCONCLUSIONS\r\nLate sampling after 3 days at the earliest, combined with reduced BacT/A incubation following the negative-to-date concept, offer an alternative opportunity to extend the shelf life of PCs from 4 to 5 days in Germany. The sensitivity of BacT/A with late sampling is nearly comparable to BF; the time-to-result is considerably longer.\r\n"}],"year":"2018","title":"Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany","issue":"7","author":[{"full_name":"Vollmer, Tanja","last_name":"Vollmer","first_name":"Tanja"},{"id":"66516","full_name":"Dabisch-Ruthe, Mareike","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe","first_name":"Mareike"},{"first_name":"Melanie","last_name":"Weinstock","full_name":"Weinstock, Melanie"},{"first_name":"Cornelius","last_name":"Knabbe","full_name":"Knabbe, Cornelius"},{"first_name":"Jens","full_name":"Dreier, Jens","last_name":"Dreier"}],"place":"Oxford [u.a.] ","type":"scientific_journal_article","intvolume":" 58","publication":"Transfusion","doi":"10.1111/trf.14617","user_id":"83781","_id":"12988","page":"1654-1664","language":[{"iso":"eng"}],"citation":{"ama":"Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion. 2018;58(7):1654-1664. doi:10.1111/trf.14617","van":"Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion. 2018;58(7):1654–64.","bjps":"Vollmer T et al. (2018) Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany. Transfusion 58, 1654–1664.","din1505-2-1":"Vollmer, Tanja ; Dabisch-Ruthe, Mareike ; Weinstock, Melanie ; Knabbe, Cornelius ; Dreier, Jens: Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. In: Transfusion Bd. 58. Oxford [u.a.] , Wiley-Blackwell (2018), Nr. 7, S. 1654–1664","ufg":"Vollmer, Tanja u. a.: Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany, in: Transfusion 58 (2018), H. 7, S. 1654–1664.","short":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Transfusion 58 (2018) 1654–1664.","chicago":"Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe, and Jens Dreier. “Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany.” Transfusion 58, no. 7 (2018): 1654–64. https://doi.org/10.1111/trf.14617.","mla":"Vollmer, Tanja, et al. “Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany.” Transfusion, vol. 58, no. 7, 2018, pp. 1654–64, https://doi.org/10.1111/trf.14617.","havard":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany, Transfusion. 58 (2018) 1654–1664.","chicago-de":"Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe und Jens Dreier. 2018. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion 58, Nr. 7: 1654–1664. doi:10.1111/trf.14617, .","apa":"Vollmer, T., Dabisch-Ruthe, M., Weinstock, M., Knabbe, C., & Dreier, J. (2018). Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion, 58(7), 1654–1664. https://doi.org/10.1111/trf.14617","ieee":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, and J. Dreier, “Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany,” Transfusion, vol. 58, no. 7, pp. 1654–1664, 2018, doi: 10.1111/trf.14617."},"publisher":"Wiley-Blackwell","status":"public"},{"date_created":"2025-06-17T07:05:46Z","date_updated":"2025-06-17T11:49:22Z","publication_status":"published","department":[{"_id":"DEP4000"},{"_id":"DEP4010"}],"year":"2018","title":"Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces.","author":[{"first_name":"Mareike","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe","full_name":"Dabisch-Ruthe, Mareike","id":"66516"},{"first_name":"Melanie","full_name":"Weinstock, Melanie","last_name":"Weinstock"},{"last_name":"Pfannebecker","id":"45690","full_name":"Pfannebecker, Jens","first_name":"Jens"},{"last_name":"Becker","id":"12640","full_name":"Becker, Barbara","first_name":"Barbara"}],"type":"conference_poster","publication":"26th International ICFMH Conference - FoodMicro 2018","doi":"10.13140/RG.2.2.12883.08484","page":"482","_id":"12989","user_id":"83781","oa":"1","language":[{"iso":"eng"}],"citation":{"chicago":"Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, and Barbara Becker. Usage of Cold Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces. 26th International ICFMH Conference - FoodMicro 2018, 2018. https://doi.org/10.13140/RG.2.2.12883.08484.","van":"Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Becker B. Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces. 26th International ICFMH Conference - FoodMicro 2018. 2018. 482 p.","mla":"Dabisch-Ruthe, Mareike, et al. “Usage of Cold Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces.” 26th International ICFMH Conference - FoodMicro 2018, 2018, https://doi.org/10.13140/RG.2.2.12883.08484.","ama":"Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Becker B. Usage of Cold Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces.; 2018. doi:10.13140/RG.2.2.12883.08484","bjps":"Dabisch-Ruthe M et al. (2018) Usage of Cold Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces. .","havard":"M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, B. Becker, Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces., 2018.","chicago-de":"Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker und Barbara Becker. 2018. Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces. 26th International ICFMH Conference - FoodMicro 2018. doi:10.13140/RG.2.2.12883.08484, .","din1505-2-1":"Dabisch-Ruthe, Mareike ; Weinstock, Melanie ; Pfannebecker, Jens ; Becker, Barbara: Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces., 2018","apa":"Dabisch-Ruthe, M., Weinstock, M., Pfannebecker, J., & Becker, B. (2018). Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces. In 26th International ICFMH Conference - FoodMicro 2018. 26th International ICFMH Conference - FoodMicro 2018, Berlin. https://doi.org/10.13140/RG.2.2.12883.08484","ufg":"Dabisch-Ruthe, Mareike u. a.: Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces., o. O. 2018.","ieee":"M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, and B. Becker, Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces. 2018. doi: 10.13140/RG.2.2.12883.08484.","short":"M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, B. Becker, Usage of Cold Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces., 2018."},"related_material":{"link":[{"relation":"confirmation","url":"https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00016749/26th-ICFM_FoodMicro2018_Book_of_Abstracts.pdf"}]},"status":"public","conference":{"start_date":"2018--09-03","end_date":"2018-09-06","location":"Berlin","name":"26th International ICFMH Conference - FoodMicro 2018"},"main_file_link":[{"open_access":"1"}]},{"publication_status":"published","_id":"12990","user_id":"83781","date_updated":"2025-06-17T08:47:43Z","language":[{"iso":"eng"}],"citation":{"din1505-2-1":"Dabisch-Ruthe, Mareike ; Weinstock, Melanie ; Pfannebecker, Jens ; Meyer, Sonja ; Schwetka, Mona ; Becker, Barbara: Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria spores on surfaces in food production. : Conventus Congressmanagement & Marketing GmbH , 2018","short":"M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka, B. Becker, Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production., Conventus Congressmanagement & Marketing GmbH , 2018.","ufg":"Dabisch-Ruthe, Mareike u. a.: Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria spores on surfaces in food production., o. O. 2018.","van":"Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Meyer S, Schwetka M, Becker B. Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria spores on surfaces in food production. 70th Annual Conference of the German Society of Hygiene and Microbiology. Conventus Congressmanagement & Marketing GmbH ; 2018.","ama":"Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Meyer S, Schwetka M, Becker B. Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production. Conventus Congressmanagement & Marketing GmbH ; 2018. doi:10.13140/RG.2.2.22949.41447","bjps":"Dabisch-Ruthe M et al. (2018) Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production. Conventus Congressmanagement & Marketing GmbH .","apa":"Dabisch-Ruthe, M., Weinstock, M., Pfannebecker, J., Meyer, S., Schwetka, M., & Becker, B. (2018). Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria spores on surfaces in food production. In 70th Annual Conference of the German Society of Hygiene and Microbiology. 70th Annual Conference of the German Society of Hygiene and Microbiology, Bochum. Conventus Congressmanagement & Marketing GmbH . https://doi.org/10.13140/RG.2.2.22949.41447","havard":"M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka, B. Becker, Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria spores on surfaces in food production., Conventus Congressmanagement & Marketing GmbH , 2018.","chicago-de":"Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, Sonja Meyer, Mona Schwetka und Barbara Becker. 2018. Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria spores on surfaces in food production. 70th Annual Conference of the German Society of Hygiene and Microbiology. Conventus Congressmanagement & Marketing GmbH . doi:10.13140/RG.2.2.22949.41447, .","ieee":"M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka, and B. Becker, Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria spores on surfaces in food production. Conventus Congressmanagement & Marketing GmbH , 2018. doi: 10.13140/RG.2.2.22949.41447.","mla":"Dabisch-Ruthe, Mareike, et al. “Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production.” 70th Annual Conference of the German Society of Hygiene and Microbiology, Conventus Congressmanagement & Marketing GmbH , 2018, https://doi.org/10.13140/RG.2.2.22949.41447.","chicago":"Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, Sonja Meyer, Mona Schwetka, and Barbara Becker. Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production. 70th Annual Conference of the German Society of Hygiene and Microbiology. Conventus Congressmanagement & Marketing GmbH , 2018. https://doi.org/10.13140/RG.2.2.22949.41447."},"publisher":"Conventus Congressmanagement & Marketing GmbH ","publication_identifier":{"isbn":["978-3-9816508-6-0"]},"publication":"70th Annual Conference of the German Society of Hygiene and Microbiology","doi":"10.13140/RG.2.2.22949.41447","date_created":"2025-06-17T07:12:33Z","conference":{"start_date":"2018-02-19","end_date":"2018-02-21","location":"Bochum","name":"70th Annual Conference of the German Society of Hygiene and Microbiology"},"title":"Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria spores on surfaces in food production.","author":[{"first_name":"Mareike","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe","id":"66516","full_name":"Dabisch-Ruthe, Mareike"},{"first_name":"Melanie","full_name":"Weinstock, Melanie","last_name":"Weinstock"},{"last_name":"Pfannebecker","id":"45690","full_name":"Pfannebecker, Jens","first_name":"Jens"},{"last_name":"Meyer","full_name":"Meyer, Sonja","id":"12829","first_name":"Sonja"},{"full_name":"Schwetka, Mona ","last_name":"Schwetka","first_name":"Mona "},{"first_name":"Barbara","last_name":"Becker","full_name":"Becker, Barbara","id":"12640"}],"status":"public","type":"conference_scientific_abstract","department":[{"_id":"DEP4010"}],"related_material":{"link":[{"relation":"confirmation","url":"https://www.dghm.org/wp-content/uploads/2018/10/DGHM2018_Abstractband_WebVersion.pdf"}]},"year":"2018"},{"date_created":"2025-06-15T10:29:56Z","publication_identifier":{"issn":["0956-7135"]},"quality_controlled":"1","date_updated":"2025-06-18T12:06:11Z","volume":80,"publication_status":"published","department":[{"_id":"DEP4010"}],"year":"2017","abstract":[{"text":"Properly handled fish is usually marketed as “fresh fish” until day 10 after fishing. About 40% of the total fishery that is used for direct human consumption is marketed in fresh form stored at temperatures up to +2 °C. Currently, there are no validated methods available for controlling the recommended period of storage. Apart from being a potential source for food fraud, spoiled fish represents a major source of foodborne illnesses and intoxications.\r\nIn this study, a rapid MALDI-TOF mass spectrometry based screening method was developed using the vitreous fluid of fish eyes as specimen for the examination of different days of storage. The vitreous fluid was collected from n = 100 freshly fished brown trouts at day 0, 3, 7, 9, and 11 post mortem (n = 20 brown trouts each day of examination). The samples were immediately measured by MALDI-TOF mass spectrometry in linear positive mode (mass range m/z 2000–20,000 Da). For quality assurance the experiment was repeated with a set of brown trouts (n = 100) originating from the same fish farm and with brown trouts (n = 100) originating from a different fish farm. For specificity testing rainbow trouts (n = 10) were examined accordingly. All obtained mass spectra were processed by means of MALDI Biotyper OC 3.1 and ClinProTools 3.0 software.\r\nThe MALDI Biotyper approach showed limited applicability for the identification of the time of storage. However, it was suitable to reliably discriminate between the closely related species brown and rainbow trout. Processing by ClinProTools revealed four crucial mass peaks (m/z 2594 Da, m/z 4857 Da, m/z 4879 Da, m/z 4899 Da) which enabled a reliable differentiation between day 0 and 3, 7, 9, 11 (rate of correct identification > 90%) as well as the differentiation between day 3 and 7, 9, 11 (rate of correct identification > 72%). However, this approach showed limited applicability within the end of the tested period of storage when comparing between day 7, 9, or 11.","lang":"eng"}],"author":[{"first_name":"Sebastian","last_name":"Ulrich","orcid":"0000-0002-4511-9537","full_name":"Ulrich, Sebastian","id":"85847"},{"first_name":"Philipp–Michael","last_name":"Beindorf","full_name":"Beindorf, Philipp–Michael"},{"full_name":"Biermaier, Barbara","last_name":"Biermaier","first_name":"Barbara"},{"last_name":"Schwaiger","full_name":"Schwaiger, Karin","first_name":"Karin"},{"full_name":"Gareis, Manfred","last_name":"Gareis","first_name":"Manfred"},{"full_name":"Gottschalk, Christoph","last_name":"Gottschalk","first_name":"Christoph"}],"issue":"10","title":"A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry","type":"scientific_journal_article","place":"Amsterdam","doi":"10.1016/j.foodcont.2017.05.005","publication":"Food Control","intvolume":" 80","page":"281-289","_id":"12975","user_id":"83781","keyword":["MALDI-TOF","Mass spectrometry","Freshness","Fish","Quality control","Authenticity"],"publisher":"Elsevier ","citation":{"ieee":"S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, and C. Gottschalk, “A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry,” Food Control, vol. 80, no. 10, pp. 281–289, 2017, doi: 10.1016/j.foodcont.2017.05.005.","apa":"Ulrich, S., Beindorf, P., Biermaier, B., Schwaiger, K., Gareis, M., & Gottschalk, C. (2017). A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. Food Control, 80(10), 281–289. https://doi.org/10.1016/j.foodcont.2017.05.005","havard":"S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, C. Gottschalk, A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry, Food Control. 80 (2017) 281–289.","chicago-de":"Ulrich, Sebastian, Philipp–Michael Beindorf, Barbara Biermaier, Karin Schwaiger, Manfred Gareis und Christoph Gottschalk. 2017. A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. Food Control 80, Nr. 10: 281–289. doi:10.1016/j.foodcont.2017.05.005, .","mla":"Ulrich, Sebastian, et al. “A Novel Approach for the Determination of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry.” Food Control, vol. 80, no. 10, 2017, pp. 281–89, https://doi.org/10.1016/j.foodcont.2017.05.005.","chicago":"Ulrich, Sebastian, Philipp–Michael Beindorf, Barbara Biermaier, Karin Schwaiger, Manfred Gareis, and Christoph Gottschalk. “A Novel Approach for the Determination of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry.” Food Control 80, no. 10 (2017): 281–89. https://doi.org/10.1016/j.foodcont.2017.05.005.","short":"S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, C. Gottschalk, Food Control 80 (2017) 281–289.","ufg":"Ulrich, Sebastian u. a.: A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry, in: Food Control 80 (2017), H. 10, S. 281–289.","din1505-2-1":"Ulrich, Sebastian ; Beindorf, Philipp–Michael ; Biermaier, Barbara ; Schwaiger, Karin ; Gareis, Manfred ; Gottschalk, Christoph: A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. In: Food Control Bd. 80. Amsterdam, Elsevier (2017), Nr. 10, S. 281–289","bjps":"Ulrich S et al. (2017) A Novel Approach for the Determination of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry. Food Control 80, 281–289.","ama":"Ulrich S, Beindorf P, Biermaier B, Schwaiger K, Gareis M, Gottschalk C. A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. Food Control. 2017;80(10):281-289. doi:10.1016/j.foodcont.2017.05.005","van":"Ulrich S, Beindorf P, Biermaier B, Schwaiger K, Gareis M, Gottschalk C. A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. Food Control. 2017;80(10):281–9."},"language":[{"iso":"eng"}],"extern":"1","status":"public"},{"intvolume":" 76","doi":"10.1016/j.foodcont.2017.01.010","publication":"Food Control","publisher":"Elsevier","language":[{"iso":"eng"}],"citation":{"bjps":"Ulrich S et al. (2017) Direct Identification of Edible Insects by MALDI-TOF Mass Spectrometry. Food Control 76, 96–101.","van":"Ulrich S, Kühn U, Biermaier B, Piacenza N, Schwaiger K, Gottschalk C, et al. Direct identification of edible insects by MALDI-TOF mass spectrometry. Food Control. 2017;76(6):96–101.","mla":"Ulrich, Sebastian, et al. “Direct Identification of Edible Insects by MALDI-TOF Mass Spectrometry.” Food Control, vol. 76, no. 6, 2017, pp. 96–101, https://doi.org/10.1016/j.foodcont.2017.01.010.","ama":"Ulrich S, Kühn U, Biermaier B, et al. Direct identification of edible insects by MALDI-TOF mass spectrometry. Food Control. 2017;76(6):96-101. doi:10.1016/j.foodcont.2017.01.010","chicago":"Ulrich, Sebastian, Ulrike Kühn, Barbara Biermaier, Nicolo Piacenza, Karin Schwaiger, Christoph Gottschalk, and Manfred Gareis. “Direct Identification of Edible Insects by MALDI-TOF Mass Spectrometry.” Food Control 76, no. 6 (2017): 96–101. https://doi.org/10.1016/j.foodcont.2017.01.010.","short":"S. Ulrich, U. Kühn, B. Biermaier, N. Piacenza, K. Schwaiger, C. Gottschalk, M. Gareis, Food Control 76 (2017) 96–101.","ieee":"S. Ulrich et al., “Direct identification of edible insects by MALDI-TOF mass spectrometry,” Food Control, vol. 76, no. 6, pp. 96–101, 2017, doi: 10.1016/j.foodcont.2017.01.010.","ufg":"Ulrich, Sebastian u. a.: Direct identification of edible insects by MALDI-TOF mass spectrometry, in: Food Control 76 (2017), H. 6, S. 96–101.","apa":"Ulrich, S., Kühn, U., Biermaier, B., Piacenza, N., Schwaiger, K., Gottschalk, C., & Gareis, M. (2017). Direct identification of edible insects by MALDI-TOF mass spectrometry. Food Control, 76(6), 96–101. https://doi.org/10.1016/j.foodcont.2017.01.010","chicago-de":"Ulrich, Sebastian, Ulrike Kühn, Barbara Biermaier, Nicolo Piacenza, Karin Schwaiger, Christoph Gottschalk und Manfred Gareis. 2017. Direct identification of edible insects by MALDI-TOF mass spectrometry. Food Control 76, Nr. 6: 96–101. doi:10.1016/j.foodcont.2017.01.010, .","havard":"S. Ulrich, U. Kühn, B. Biermaier, N. Piacenza, K. Schwaiger, C. Gottschalk, M. Gareis, Direct identification of edible insects by MALDI-TOF mass spectrometry, Food Control. 76 (2017) 96–101.","din1505-2-1":"Ulrich, Sebastian ; Kühn, Ulrike ; Biermaier, Barbara ; Piacenza, Nicolo ; Schwaiger, Karin ; Gottschalk, Christoph ; Gareis, Manfred: Direct identification of edible insects by MALDI-TOF mass spectrometry. In: Food Control Bd. 76. Amsterdam, Elsevier (2017), Nr. 6, S. 96–101"},"page":"96-101","keyword":["MALDI-TOF MS","Mass spectrometry","Edible insects","Authenticity","Food quality"],"_id":"12976","user_id":"83781","extern":"1","status":"public","quality_controlled":"1","date_created":"2025-06-15T10:31:37Z","publication_identifier":{"issn":["0956-7135"]},"date_updated":"2025-06-18T12:08:47Z","volume":76,"publication_status":"published","year":"2017","abstract":[{"text":"The consumption of edible insects (entomophagy) will gain greater significance facing the increasing global population, which is suggested to reach 9 billion people in 2050 (FAO., 2009). Due to their high amount of proteins, fatty acids, vitamins, and minerals insects represent a valuable source of essential nutrients.\r\nWhile the consumption of insects is very common in many countries of Africa and Asia, there is a far smaller acceptance for entomophagy in Western cultures. Though, products such as noodles or burger paddies made from insect meal have a better compliance and can already be purchased in some countries of the European Union. This processing step however involves the risk of adulteration, because there is no more possibility to authenticate the insects once they are ground.\r\nThe aim of this study was to investigate whether edible insects could be measured and distinguished by MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry). Therefore, different kinds of edible insects (buffalo worms, mealworms, crickets and grasshoppers) were purchased via online shops and ground subsequently. The insect powder was extracted by vigorously shaking in diluted formic acid and measured by MALDI-TOF MS. The measurement provided reproducible as well as specific mass spectra and enabled a precise differentiation of the different species.","lang":"eng"}],"department":[{"_id":"DEP4010"}],"place":"Amsterdam","type":"scientific_journal_article","title":"Direct identification of edible insects by MALDI-TOF mass spectrometry","issue":"6","author":[{"id":"85847","full_name":"Ulrich, Sebastian","last_name":"Ulrich","orcid":"0000-0002-4511-9537","first_name":"Sebastian"},{"first_name":"Ulrike","full_name":"Kühn, Ulrike","last_name":"Kühn"},{"first_name":"Barbara","full_name":"Biermaier, Barbara","last_name":"Biermaier"},{"first_name":"Nicolo","full_name":"Piacenza, Nicolo","last_name":"Piacenza"},{"first_name":"Karin","last_name":"Schwaiger","full_name":"Schwaiger, Karin"},{"first_name":"Christoph","full_name":"Gottschalk, Christoph","last_name":"Gottschalk"},{"full_name":"Gareis, Manfred","last_name":"Gareis","first_name":"Manfred"}]},{"related_material":{"link":[{"url":"https://www.behrs.de/titel/lebensmittelassoziierte-viren-norovirus/171","relation":"confirmation"}]},"year":2016,"department":[{"_id":"DEP4010"}],"type":"book","title":"Lebensmittelassoziierte Viren - Norovirus ","author":[{"full_name":"Becker, Barbara","id":"12640","last_name":"Becker","first_name":"Barbara"},{"first_name":"Jens","id":"45690","full_name":"Pfannebecker, Jens","last_name":"Pfannebecker"}],"status":"public","publication_identifier":{"isbn":["978-3-95468-388-8"]},"edition":"1","date_created":"2020-05-18T20:38:33Z","language":[{"iso":"ger"}],"citation":{"ufg":"Becker, Barbara/Pfannebecker, Jens (2016): Lebensmittelassoziierte Viren - Norovirus (=Lebensmittelassoziierte Viren - Norovirus ), 1.","ieee":"B. Becker and J. Pfannebecker, Lebensmittelassoziierte Viren - Norovirus , 1st ed. Behr`s Verlag, 2016.","short":"B. Becker, J. Pfannebecker, Lebensmittelassoziierte Viren - Norovirus , 1st ed., Behr`s Verlag, 2016.","din1505-2-1":"Becker, Barbara ; Pfannebecker, Jens: Lebensmittelassoziierte Viren - Norovirus , Lebensmittelassoziierte Viren - Norovirus . 1. Aufl. : Behr`s Verlag, 2016","havard":"B. Becker, J. Pfannebecker, Lebensmittelassoziierte Viren - Norovirus , 1st ed., Behr`s Verlag, 2016.","chicago-de":"Becker, Barbara und Jens Pfannebecker. 2016. Lebensmittelassoziierte Viren - Norovirus . 1. Aufl. Lebensmittelassoziierte Viren - Norovirus . Behr`s Verlag.","apa":"Becker, B., & Pfannebecker, J. (2016). Lebensmittelassoziierte Viren - Norovirus (1st ed.). Behr`s Verlag.","bjps":"Becker B and Pfannebecker J (2016) Lebensmittelassoziierte Viren - Norovirus , 1st ed. Behr`s Verlag.","chicago":"Becker, Barbara, and Jens Pfannebecker. Lebensmittelassoziierte Viren - Norovirus . 1st ed. Lebensmittelassoziierte Viren - Norovirus . Behr`s Verlag, 2016.","ama":"Becker B, Pfannebecker J. Lebensmittelassoziierte Viren - Norovirus . 1st ed. Behr`s Verlag; 2016.","mla":"Becker, Barbara, and Jens Pfannebecker. Lebensmittelassoziierte Viren - Norovirus . 1st ed., Behr`s Verlag, 2016.","van":"Becker B, Pfannebecker J. Lebensmittelassoziierte Viren - Norovirus . 1st ed. Behr`s Verlag; 2016. 80 p. (Lebensmittelassoziierte Viren - Norovirus )."},"publisher":"Behr`s Verlag","series_title":"Lebensmittelassoziierte Viren - Norovirus ","_id":"2379","user_id":"74222","date_updated":"2023-03-15T13:49:39Z","page":"80"},{"extern":"1","status":"public","intvolume":" 408","publication":" Analytical & bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry, Analusis and Quimica analitica","doi":"10.1007/s00216-016-9800-9","_id":"12977","user_id":"83781","keyword":["Stachybotrys spp","MALDI-TOF MS","Mass spectrometry","Filamentous fungi"],"page":"7565-7581","language":[{"iso":"eng"}],"citation":{"mla":"Ulrich, Sebastian, et al. “Identification of Stachybotrys Spp. by MALDI-TOF Mass Spectrometry.” Analytical & Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica, vol. 408, no. 27, 2016, pp. 7565–81, https://doi.org/10.1007/s00216-016-9800-9.","chicago":"Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis, and Christoph Gottschalk. “Identification of Stachybotrys Spp. by MALDI-TOF Mass Spectrometry.” Analytical & Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica 408, no. 27 (2016): 7565–81. https://doi.org/10.1007/s00216-016-9800-9.","apa":"Ulrich, S., Biermaier, B., Bader, O., Wolf, G., Straubinger, R. K., Didier, A., Sperner, B., Schwaiger, K., Gareis, M., & Gottschalk, C. (2016). Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. Analytical & Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica, 408(27), 7565–7581. https://doi.org/10.1007/s00216-016-9800-9","havard":"S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier, B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry, Analytical & Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica. 408 (2016) 7565–7581.","chicago-de":"Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis und Christoph Gottschalk. 2016. Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. Analytical & bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica 408, Nr. 27: 7565–7581. doi:10.1007/s00216-016-9800-9, .","ieee":"S. Ulrich et al., “Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry,” Analytical & bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica, vol. 408, no. 27, pp. 7565–7581, 2016, doi: 10.1007/s00216-016-9800-9.","ama":"Ulrich S, Biermaier B, Bader O, et al. Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. Analytical & bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica. 2016;408(27):7565-7581. doi:10.1007/s00216-016-9800-9","van":"Ulrich S, Biermaier B, Bader O, Wolf G, Straubinger RK, Didier A, et al. Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. Analytical & bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica. 2016;408(27):7565–81.","bjps":"Ulrich S et al. (2016) Identification of Stachybotrys Spp. by MALDI-TOF Mass Spectrometry. Analytical & bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica 408, 7565–7581.","din1505-2-1":"Ulrich, Sebastian ; Biermaier, Barbara ; Bader, Oliver ; Wolf, Georg ; Straubinger, Reinhard K. ; Didier, Andrea ; Sperner, Brigitte ; Schwaiger, Karin ; u. a.: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. In: Analytical & bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica Bd. 408. Berlin ; Heidelberg, Springer (2016), Nr. 27, S. 7565–7581","short":"S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier, B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Analytical & Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica 408 (2016) 7565–7581.","ufg":"Ulrich, Sebastian u. a.: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry, in: Analytical & bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica 408 (2016), H. 27, S. 7565–7581."},"publisher":"Springer","department":[{"_id":"DEP4010"}],"abstract":[{"text":"Stachybotrys (S.) spp. are omnipresent cellulolytic molds. Some species are highly toxic owing to their ability to synthesize various secondary metabolites such as macrocyclic trichothecenes or hemolysins. The reliable identification of Stachybotrys at species level is currently limited to genome-based identification. This study aimed to establish a fast and reliable MALDI-TOF MS identification method by optimizing the pre-analytical steps for protein extraction for subsequent generation of high-quality fingerprint mass spectra. Eight reference strains of the American Type Culture Collection and the Technical University of Denmark were cultivated in triplicate (biological repetitions) for 2 days in malt extract broth. The mycelia (1.5 ml) were first washed with 75 % ethanol and an additional washing step with dimethyl sulfoxide (10 %) was added to remove unspecific low weight masses. Furthermore, mycelia were broken with roughened glass beads in formic acid (70 %) and acetonitrile. The method was successfully applied to a total of 45 isolates of Stachybotrys originating from three different habitats (indoor, feed, and food samples; n = 15 each): Twenty-seven isolates of S. chartarum and 18 isolates of S. chlorohalonata could be identified by MALDI-TOF MS. The data obtained exactly matched those obtained by genome-based identification. The mean score values for S. chartarum ranged from 2.509 to 2.739 and from 2.148 to 2.622 for S. chlorohalonata with a very good reproducibility: the relative standard deviations were between 0.3 % and 6.8 %. Thus, MALDI-TOF MS proved to be a fast and reliable alternative to identification of Stachybotrys spp. by nucleotide amplification and sequencing.","lang":"eng"}],"year":"2016","title":"Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry","issue":"27","author":[{"orcid":"0000-0002-4511-9537","last_name":"Ulrich","id":"85847","full_name":"Ulrich, Sebastian","first_name":"Sebastian"},{"first_name":"Barbara","last_name":"Biermaier","full_name":"Biermaier, Barbara"},{"last_name":"Bader","full_name":"Bader, Oliver","first_name":"Oliver"},{"first_name":"Georg","full_name":"Wolf, Georg","last_name":"Wolf"},{"first_name":"Reinhard K.","last_name":"Straubinger","full_name":"Straubinger, Reinhard K."},{"full_name":"Didier, Andrea","last_name":"Didier","first_name":"Andrea"},{"full_name":"Sperner, Brigitte","last_name":"Sperner","first_name":"Brigitte"},{"first_name":"Karin","full_name":"Schwaiger, Karin","last_name":"Schwaiger"},{"first_name":"Manfred","full_name":"Gareis, Manfred","last_name":"Gareis"},{"last_name":"Gottschalk","full_name":"Gottschalk, Christoph","first_name":"Christoph"}],"place":"Berlin ; Heidelberg","type":"scientific_journal_article","publication_identifier":{"issn":["1618-2642"],"eissn":["1618-2650"]},"date_created":"2025-06-15T10:32:52Z","quality_controlled":"1","publication_status":"published","volume":408,"date_updated":"2025-06-18T12:11:46Z"},{"status":"public","file_date_updated":"2024-12-02T14:22:01Z","ddc":["570","572"],"file":[{"date_created":"2020-05-18T09:25:39Z","relation":"main_file","creator":"z5h-0dr","file_size":2314359,"file_id":"2358","date_updated":"2024-12-02T14:22:01Z","file_name":"BECKER(1).pdf","access_level":"closed","content_type":"application/pdf"}],"citation":{"short":"B. Becker, H. Nerenz, J.M. Quadflieg, A. Schrader, V. Becker, SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe 140 (2015) 10–17.","ufg":"Becker, Barbara u. a.: Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik, in: SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe 140 (2015), H. 11, S. 10–17.","din1505-2-1":"Becker, Barbara ; Nerenz, Heiko ; Quadflieg, Jutta Maria ; Schrader, Andreas ; Becker, Verena : Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik. In: SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe Bd. 140, Verl. für Chemische Industrie, Ziolkowsky (2015), Nr. 11, S. 10–17","bjps":"Becker B et al. (2015) Phtoalexine Als Antimikrobielle Pflanzenwirkstoffe Für Die Kosmetik. SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe 140, 10–17.","van":"Becker B, Nerenz H, Quadflieg JM, Schrader A, Becker V. Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik. SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe. 2015;140(11):10–7.","ama":"Becker B, Nerenz H, Quadflieg JM, Schrader A, Becker V. Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik. SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe. 2015;140(11):10-17.","ieee":"B. Becker, H. Nerenz, J. M. Quadflieg, A. Schrader, and V. Becker, “Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik,” SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe, vol. 140, no. 11, pp. 10–17, 2015.","apa":"Becker, B., Nerenz, H., Quadflieg, J. M., Schrader, A., & Becker, V. (2015). Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik. SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe, 140(11), 10–17.","havard":"B. Becker, H. Nerenz, J.M. Quadflieg, A. Schrader, V. Becker, Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik, SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe. 140 (2015) 10–17.","chicago-de":"Becker, Barbara, Heiko Nerenz, Jutta Maria Quadflieg, Andreas Schrader und Verena Becker. 2015. Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik. SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe 140, Nr. 11: 10–17.","mla":"Becker, Barbara, et al. “Phtoalexine Als Antimikrobielle Pflanzenwirkstoffe Für Die Kosmetik.” SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe, vol. 140, no. 11, 2015, pp. 10–17.","chicago":"Becker, Barbara, Heiko Nerenz, Jutta Maria Quadflieg, Andreas Schrader, and Verena Becker. “Phtoalexine Als Antimikrobielle Pflanzenwirkstoffe Für Die Kosmetik.” SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe 140, no. 11 (2015): 10–17."},"language":[{"iso":"eng"}],"publisher":"Verl. für Chemische Industrie, Ziolkowsky","_id":"2357","user_id":"83781","page":"10-17","publication":"SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe","intvolume":" 140","type":"journal_article","author":[{"last_name":"Becker","full_name":"Becker, Barbara","id":"12640","first_name":"Barbara"},{"first_name":"Heiko ","last_name":"Nerenz","full_name":"Nerenz, Heiko "},{"first_name":"Jutta Maria","last_name":"Quadflieg","full_name":"Quadflieg, Jutta Maria"},{"last_name":"Schrader","full_name":"Schrader, Andreas","first_name":"Andreas"},{"first_name":"Verena ","full_name":"Becker, Verena ","last_name":"Becker"}],"issue":"11","title":"Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik","year":"2015","department":[{"_id":"DEP4010"}],"publication_status":"published","volume":140,"date_updated":"2024-12-02T14:22:03Z","has_accepted_license":"1","publication_identifier":{"unknown":["0942-7694"]},"date_created":"2020-05-18T09:25:44Z"},{"date_created":"2025-06-15T10:34:57Z","publication_identifier":{"eissn":["1477-2817"],"unknown":["0003-942X "],"issn":["1745-039X"]},"quality_controlled":"1","date_updated":"2025-06-18T12:18:46Z","publication_status":"published","volume":70,"department":[{"_id":"DEP4010"}],"year":"2015","abstract":[{"lang":"eng","text":"Diet change and fatness are supposed to challenge the immune system of the cow. Therefore, immunological and haematological consequences of adaptation to and continued feeding of a high-energy diet were studied in eight non-pregnant, non-lactating Holstein cows over 16 weeks. Blood haptoglobin concentration remained unaltered, suggesting that an acute phase reaction was not induced. Stimulation ability of peripheral blood mononuclear cells and stimulated oxidative burst capacity of granulocytes increased significantly in the course of the experiment after an initial drop. While total leucocyte counts increased, the proportion of granulocytes increased and that of lymphocytes decreased at the same time as the ratio of CD4+/CD8+ lymphocytes did. Capability of rumen microbes to detoxify the immune-modulating mycotoxin deoxynivalenol (DON) was not compromised as indicated by the exclusive presence of de-DON as the detoxified DON metabolite in blood. In conclusion, both diet change and prolonged positive energy balance influenced the bovine immune system."}],"author":[{"last_name":"Dänicke","full_name":"Dänicke, Sven","first_name":"Sven"},{"full_name":"Meyer, Ulrich","last_name":"Meyer","first_name":"Ulrich"},{"last_name":"Winkler","full_name":"Winkler, Janine","first_name":"Janine"},{"id":"85847","full_name":"Ulrich, Sebastian","orcid":"0000-0002-4511-9537","last_name":"Ulrich","first_name":"Sebastian"},{"first_name":"Jana","full_name":"Frahm, Jana","last_name":"Frahm"},{"last_name":"Kersten","full_name":"Kersten, Susanne","first_name":"Susanne"},{"last_name":"Valenta","full_name":"Valenta, Hana","first_name":"Hana"},{"first_name":"Jürgen","full_name":"Rehage, Jürgen","last_name":"Rehage"},{"last_name":"Häussler","full_name":"Häussler, Susanne","first_name":"Susanne"},{"last_name":"Sauerwein","full_name":"Sauerwein, Helga","first_name":"Helga"},{"first_name":"Lena","last_name":"Locher","full_name":"Locher, Lena"}],"issue":"1","title":"Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins","type":"scientific_journal_article","place":"Abingdon","doi":"10.1080/1745039x.2015.1117561","publication":"Archives of Animal Nutrition","intvolume":" 70","page":"1-16","_id":"12978","user_id":"83781","keyword":["Dairy cowsdeoxynivalenol","energy consumption","functional responses","haematology","leucocytes","mycotoxins","zearalenone"],"publisher":"Taylor & Francis","citation":{"apa":"Dänicke, S., Meyer, U., Winkler, J., Ulrich, S., Frahm, J., Kersten, S., Valenta, H., Rehage, J., Häussler, S., Sauerwein, H., & Locher, L. (2015). Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins. Archives of Animal Nutrition, 70(1), 1–16. https://doi.org/10.1080/1745039x.2015.1117561","havard":"S. Dänicke, U. Meyer, J. Winkler, S. Ulrich, J. Frahm, S. Kersten, H. Valenta, J. Rehage, S. Häussler, H. Sauerwein, L. Locher, Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins, Archives of Animal Nutrition. 70 (2015) 1–16.","chicago-de":"Dänicke, Sven, Ulrich Meyer, Janine Winkler, Sebastian Ulrich, Jana Frahm, Susanne Kersten, Hana Valenta, u. a. 2015. Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins. Archives of Animal Nutrition 70, Nr. 1: 1–16. doi:10.1080/1745039x.2015.1117561, .","ieee":"S. Dänicke et al., “Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins,” Archives of Animal Nutrition, vol. 70, no. 1, pp. 1–16, 2015, doi: 10.1080/1745039x.2015.1117561.","mla":"Dänicke, Sven, et al. “Haematological and Immunological Adaptations of Non-Pregnant, Non-Lactating Dairy Cows to a High-Energetic Diet Containing Mycotoxins.” Archives of Animal Nutrition, vol. 70, no. 1, 2015, pp. 1–16, https://doi.org/10.1080/1745039x.2015.1117561.","chicago":"Dänicke, Sven, Ulrich Meyer, Janine Winkler, Sebastian Ulrich, Jana Frahm, Susanne Kersten, Hana Valenta, et al. “Haematological and Immunological Adaptations of Non-Pregnant, Non-Lactating Dairy Cows to a High-Energetic Diet Containing Mycotoxins.” Archives of Animal Nutrition 70, no. 1 (2015): 1–16. https://doi.org/10.1080/1745039x.2015.1117561.","din1505-2-1":"Dänicke, Sven ; Meyer, Ulrich ; Winkler, Janine ; Ulrich, Sebastian ; Frahm, Jana ; Kersten, Susanne ; Valenta, Hana ; Rehage, Jürgen ; u. a.: Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins. In: Archives of Animal Nutrition Bd. 70. Abingdon, Taylor & Francis (2015), Nr. 1, S. 1–16","short":"S. Dänicke, U. Meyer, J. Winkler, S. Ulrich, J. Frahm, S. Kersten, H. Valenta, J. Rehage, S. Häussler, H. Sauerwein, L. Locher, Archives of Animal Nutrition 70 (2015) 1–16.","ufg":"Dänicke, Sven u. a.: Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins, in: Archives of Animal Nutrition 70 (2015), H. 1, S. 1–16.","van":"Dänicke S, Meyer U, Winkler J, Ulrich S, Frahm J, Kersten S, et al. Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins. Archives of Animal Nutrition. 2015;70(1):1–16.","ama":"Dänicke S, Meyer U, Winkler J, et al. Haematological and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins. Archives of Animal Nutrition. 2015;70(1):1-16. doi:10.1080/1745039x.2015.1117561","bjps":"Dänicke S et al. (2015) Haematological and Immunological Adaptations of Non-Pregnant, Non-Lactating Dairy Cows to a High-Energetic Diet Containing Mycotoxins. Archives of Animal Nutrition 70, 1–16."},"language":[{"iso":"eng"}],"extern":"1","status":"public"},{"year":2014,"department":[{"_id":"DEP4010"}],"type":"newspaper_article","author":[{"id":"12640","full_name":"Becker, Barbara","last_name":"Becker","first_name":"Barbara"}],"status":"public","issue":"5","title":"useful for beverage spoilage microoganism","publication":"Brauwelt International","publication_identifier":{"unknown":["0934-9340"]},"date_created":"2020-05-18T10:12:34Z","citation":{"ufg":"Becker, Barbara (2014): useful for beverage spoilage microoganism, in: Brauwelt International (5) (2014), S. 252–256.","short":"B. Becker, Brauwelt International (2014) 252–256.","din1505-2-1":"Becker, Barbara: useful for beverage spoilage microoganism. In: Brauwelt International (2014), Nr. 5","bjps":"Becker B (2014) Useful for Beverage Spoilage Microoganism. Brauwelt International, 252–256.","ama":"Becker B. useful for beverage spoilage microoganism. Brauwelt International. 2014:252-256.","van":"Becker B. useful for beverage spoilage microoganism. Brauwelt International. 2014;252–6.","ieee":"B. Becker, “useful for beverage spoilage microoganism,” Brauwelt International, no. 5, pp. 252–256, 2014.","chicago-de":"Becker, Barbara. 2014. useful for beverage spoilage microoganism. Brauwelt International.","havard":"B. Becker, useful for beverage spoilage microoganism, Brauwelt International. (2014) 252–256.","apa":"Becker, B. (2014). useful for beverage spoilage microoganism. Brauwelt International, pp. 252–256.","chicago":"Becker, Barbara. “Useful for Beverage Spoilage Microoganism.” Brauwelt International, 2014.","mla":"Becker, Barbara. “Useful for Beverage Spoilage Microoganism.” Brauwelt International, no. 5, 2014, pp. 252–56."},"language":[{"iso":"eng"}],"_id":"2363","publication_date":"2014","user_id":"74222","page":"252-256","date_updated":"2023-03-15T13:49:39Z"},{"has_accepted_license":"1","publication_identifier":{"issn":["0942-7694"]},"date_created":"2020-05-20T07:28:39Z","publication_status":"published","volume":140,"date_updated":"2024-12-02T14:21:46Z","year":"2014","department":[{"_id":"DEP4010"}],"type":"journal_article","author":[{"id":"12640","full_name":"Becker, Barbara","last_name":"Becker","first_name":"Barbara"},{"last_name":"Becker","full_name":"Becker, Verena","first_name":"Verena"},{"last_name":"Nerenz","full_name":"Nerenz, Heiko","first_name":"Heiko"},{"full_name":"Schrader, Andreas","last_name":"Schrader","first_name":"Andreas"}],"issue":"5","title":"Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten","publication":"SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe","intvolume":" 140","citation":{"short":"B. Becker, V. Becker, H. Nerenz, A. Schrader, SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe 140 (2014) 26–27, 30.","ufg":"Becker, Barbara u. a.: Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten, in: SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe 140 (2014), H. 5, S. 26–27, 30.","ieee":"B. Becker, V. Becker, H. Nerenz, and A. Schrader, “Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten,” SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe, vol. 140, no. 5, pp. 26–27, 30, 2014.","apa":"Becker, B., Becker, V., Nerenz, H., & Schrader, A. (2014). Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe, 140(5), 26–27, 30.","din1505-2-1":"Becker, Barbara ; Becker, Verena ; Nerenz, Heiko ; Schrader, Andreas: Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. In: SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe Bd. 140, Verl. für Chemische Industrie, Ziolkowsky (2014), Nr. 5, S. 26–27, 30","chicago-de":"Becker, Barbara, Verena Becker, Heiko Nerenz und Andreas Schrader. 2014. Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe 140, Nr. 5: 26–27, 30.","havard":"B. Becker, V. Becker, H. Nerenz, A. Schrader, Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten, SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe. 140 (2014) 26–27, 30.","bjps":"Becker B et al. (2014) Einsatz Der Durchflusszytometrie Zum Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten. SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe 140, 26–27, 30.","ama":"Becker B, Becker V, Nerenz H, Schrader A. Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe. 2014;140(5):26-27, 30.","mla":"Becker, Barbara, et al. “Einsatz Der Durchflusszytometrie Zum Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten.” SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe, vol. 140, no. 5, 2014, pp. 26–27, 30.","van":"Becker B, Becker V, Nerenz H, Schrader A. Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe. 2014;140(5):26–7, 30.","chicago":"Becker, Barbara, Verena Becker, Heiko Nerenz, and Andreas Schrader. “Einsatz Der Durchflusszytometrie Zum Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten.” SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe 140, no. 5 (2014): 26–27, 30."},"language":[{"iso":"eng"}],"publisher":"Verl. für Chemische Industrie, Ziolkowsky","_id":"2403","user_id":"83781","page":"26 - 27, 30","ddc":["610","660"],"file":[{"date_updated":"2024-12-02T14:21:45Z","access_level":"closed","content_type":"application/pdf","file_name":"becker(2).pdf","file_size":3196553,"date_created":"2020-05-20T07:37:08Z","relation":"main_file","creator":"z5h-0dr","file_id":"2405"}],"status":"public","file_date_updated":"2024-12-02T14:21:45Z"},{"doi":"10.1080/1745039x.2014.973243","publication":"Archives of Animal Nutrition","intvolume":" 68","_id":"12979","user_id":"83781","keyword":["blood chemistry","dairy cows","endotoxins","energy balance","energy content","rumen fermentation"],"page":"460-477","citation":{"apa":"Dänicke, S., Meyer, U., Winkler, J., Schulz, K., Ulrich, S., Frahm, J., Kersten, S., Rehage, J., Breves, G., Häußler, S., Sauerwein, H., & Locher, L. (2014). Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. Archives of Animal Nutrition, 68(6), 460–477. https://doi.org/10.1080/1745039x.2014.973243","chicago-de":"Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian Ulrich, Jana Frahm, Susanne Kersten, u. a. 2014. Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. Archives of Animal Nutrition 68, Nr. 6: 460–477. doi:10.1080/1745039x.2014.973243, .","havard":"S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten, J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity, Archives of Animal Nutrition. 68 (2014) 460–477.","ieee":"S. Dänicke et al., “Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity,” Archives of Animal Nutrition, vol. 68, no. 6, pp. 460–477, 2014, doi: 10.1080/1745039x.2014.973243.","mla":"Dänicke, Sven, et al. “Description of a Bovine Model for Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or Gravidity.” Archives of Animal Nutrition, vol. 68, no. 6, 2014, pp. 460–77, https://doi.org/10.1080/1745039x.2014.973243.","chicago":"Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian Ulrich, Jana Frahm, Susanne Kersten, et al. “Description of a Bovine Model for Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or Gravidity.” Archives of Animal Nutrition 68, no. 6 (2014): 460–77. https://doi.org/10.1080/1745039x.2014.973243.","din1505-2-1":"Dänicke, Sven ; Meyer, Ulrich ; Winkler, Janine ; Schulz, Kirsten ; Ulrich, Sebastian ; Frahm, Jana ; Kersten, Susanne ; Rehage, Jürgen ; u. a.: Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. In: Archives of Animal Nutrition Bd. 68. Abingdon, Taylor & Francis (2014), Nr. 6, S. 460–477","short":"S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten, J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Archives of Animal Nutrition 68 (2014) 460–477.","ufg":"Dänicke, Sven u. a.: Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity, in: Archives of Animal Nutrition 68 (2014), H. 6, S. 460–477.","van":"Dänicke S, Meyer U, Winkler J, Schulz K, Ulrich S, Frahm J, et al. Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. Archives of Animal Nutrition. 2014;68(6):460–77.","ama":"Dänicke S, Meyer U, Winkler J, et al. Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. Archives of Animal Nutrition. 2014;68(6):460-477. doi:10.1080/1745039x.2014.973243","bjps":"Dänicke S et al. (2014) Description of a Bovine Model for Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or Gravidity. Archives of Animal Nutrition 68, 460–477."},"language":[{"iso":"eng"}],"publisher":"Taylor & Francis","extern":"1","status":"public","publication_identifier":{"eissn":["1477-2817"],"issn":["1745-039X","0003-942X"]},"date_created":"2025-06-15T10:36:14Z","quality_controlled":"1","volume":68,"publication_status":"published","date_updated":"2025-06-18T12:23:01Z","department":[{"_id":"DEP4010"}],"abstract":[{"lang":"eng","text":"Physiological consequences of adaptation to and continued feeding of a high-energetic diet were studied in eight non-pregnant, non-lactating dairy Holstein cows over a period of 16 weeks. The first six weeks served as an adaptation period from the low energetic straw-based diet (3.8 MJ NEL/kg DM) to the high-energetic ration (7.5 MJ NEL/kg DM). Intake of dry matter (DM) increased with dietary energy concentration from 9 to 20 kg/d up to week 9 to 12 and decreased thereafter. The initial live weight (LW) of 550 ± 60 kg was increased linearly and corresponded to an average daily LW gain of 2.3 ± 0.3 kg. Energy balance increased approximately nine-fold to a maximum of 114 MJ NEL/d in week 10. Ruminal fermentation pattern was completely changed from an acetate dominating profile to a propionate based one, which was paralleled by a marked increase in the rumen fluid endotoxin concentration. Unlike blood glucose concentration, which increased continuously, that of cholesterol and triglycerides started to increase after an initial stagnation. In conclusion, both ruminal adaptation to a high-energetic diet and the continued feeding of such a diet induced digestive and metabolic adaptations in non-pregnant, non-lactating cows characterised by a progressing positive energy balance."}],"year":"2014","author":[{"full_name":"Dänicke, Sven","last_name":"Dänicke","first_name":"Sven"},{"first_name":"Ulrich","full_name":"Meyer, Ulrich","last_name":"Meyer"},{"first_name":"Janine","full_name":"Winkler, Janine","last_name":"Winkler"},{"last_name":"Schulz","full_name":"Schulz, Kirsten","first_name":"Kirsten"},{"first_name":"Sebastian","full_name":"Ulrich, Sebastian","id":"85847","orcid":"0000-0002-4511-9537","last_name":"Ulrich"},{"full_name":"Frahm, Jana","last_name":"Frahm","first_name":"Jana"},{"first_name":"Susanne","full_name":"Kersten, Susanne","last_name":"Kersten"},{"first_name":"Jürgen","full_name":"Rehage, Jürgen","last_name":"Rehage"},{"last_name":"Breves","full_name":"Breves, Gerhard","first_name":"Gerhard"},{"first_name":"Susanne","last_name":"Häußler","full_name":"Häußler, Susanne"},{"first_name":"Helga","full_name":"Sauerwein, Helga","last_name":"Sauerwein"},{"last_name":"Locher","full_name":"Locher, Lena","first_name":"Lena"}],"issue":"6","title":"Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity","type":"scientific_journal_article","place":"Abingdon"},{"year":"2014","abstract":[{"text":"Background\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder characterized by late onset and progressive calcification of elastic fibers in skin, eyes and the cardiovascular system, exemplifying a model for conditions characterized by soft tissue calcification.\r\nObjective\r\nThe aim of our study was to characterize cellular inorganic pyrophosphate (PPi) homeostasis in PXE.\r\nMethods\r\nGene expression of PPi metabolizing enzymes was determined by quantitative real-time PCR after incubation up to 21 days with or without addition of Na2HPO4. Extracellular and cytosolic PPi concentrations were measured by enzyme-linked bioluminescence assay. ALP and ENPP1 activity was determined spectrophotometrically. We further established a human cell culture model suitable for investigating PXE and related disorders without addition of artificial calcification triggers.\r\nResults\r\nIndependently of the experimental conditions, PXE fibroblasts revealed a higher degree of matrix calcification. We observed that matrix calcification was associated with altered gene expression of PPi metabolizing enzymes in PXE fibroblasts. In this context, PXE fibroblasts exhibited significantly higher expression of ALP and OPN and reduced mRNA expression and activity of ENPP1. Here, for the first time cytosolic and extracellular PPi levels were shown to be strongly reduced in PXE fibroblasts. We further showed that PPi concentration in bovine and human sera additives had a strong impact on matrix calcification. In a last experimental line, we demonstrated that addition of PPi analogs reduced matrix calcification of PXE fibroblasts most likely by reducing ALP and OPN mRNA expression, restoring ENPP1 activity and subsequently elevating PPi concentrations.\r\nConclusion\r\nThe results of our study along with recent findings point to the essential role of PPi as the central regulatory metabolites preventing matrix calcification in PXE. But what remains to be determined is the underlying molecular mechanism leading to depletion of PPi in PXE. We further suggest that supplementation of PPi analogs might counteract pathological calcification in PXE and related disorders.","lang":"eng"}],"department":[{"_id":"DEP4010"}],"place":"Amsterdam","type":"scientific_journal_article","issue":"2","title":"Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum","author":[{"first_name":"Mareike","last_name":"Dabisch-Ruthe","orcid":"https://orcid.org/0009-0008-7644-0826","full_name":"Dabisch-Ruthe, Mareike","id":"66516"},{"last_name":"Kuzaj","full_name":"Kuzaj, Patricia","first_name":"Patricia"},{"full_name":"Götting, Christian","last_name":"Götting","first_name":"Christian"},{"first_name":"Cornelius","full_name":"Knabbe, Cornelius","last_name":"Knabbe"},{"first_name":"Doris","last_name":"Hendig","full_name":"Hendig, Doris"}],"quality_controlled":"1","date_created":"2025-06-17T06:52:59Z","publication_identifier":{"issn":["0923-1811"],"eissn":["1873-569X"]},"date_updated":"2025-06-17T13:48:41Z","volume":75,"publication_status":"published","status":"public","intvolume":" 75","doi":"10.1016/j.jdermsci.2014.04.015","publication":"Journal of Dermatological Science","publisher":"Elsevier ","language":[{"iso":"eng"}],"citation":{"van":"Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal of Dermatological Science. 2014;75(2):109–20.","ama":"Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal of Dermatological Science. 2014;75(2):109-120. doi:10.1016/j.jdermsci.2014.04.015","bjps":"Dabisch-Ruthe M et al. (2014) Pyrophosphates as a Major Inhibitor of Matrix Calcification in Pseudoxanthoma Elasticum. Journal of Dermatological Science 75, 109–120.","din1505-2-1":"Dabisch-Ruthe, Mareike ; Kuzaj, Patricia ; Götting, Christian ; Knabbe, Cornelius ; Hendig, Doris: Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. In: Journal of Dermatological Science Bd. 75. Amsterdam, Elsevier (2014), Nr. 2, S. 109–120","ufg":"Dabisch-Ruthe, Mareike u. a.: Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum, in: Journal of Dermatological Science 75 (2014), H. 2, S. 109–120.","short":"M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Journal of Dermatological Science 75 (2014) 109–120.","chicago":"Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius Knabbe, and Doris Hendig. “Pyrophosphates as a Major Inhibitor of Matrix Calcification in Pseudoxanthoma Elasticum.” Journal of Dermatological Science 75, no. 2 (2014): 109–20. https://doi.org/10.1016/j.jdermsci.2014.04.015.","mla":"Dabisch-Ruthe, Mareike, et al. “Pyrophosphates as a Major Inhibitor of Matrix Calcification in Pseudoxanthoma Elasticum.” Journal of Dermatological Science, vol. 75, no. 2, 2014, pp. 109–20, https://doi.org/10.1016/j.jdermsci.2014.04.015.","havard":"M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum, Journal of Dermatological Science. 75 (2014) 109–120.","chicago-de":"Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius Knabbe und Doris Hendig. 2014. Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal of Dermatological Science 75, Nr. 2: 109–120. doi:10.1016/j.jdermsci.2014.04.015, .","apa":"Dabisch-Ruthe, M., Kuzaj, P., Götting, C., Knabbe, C., & Hendig, D. (2014). Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal of Dermatological Science, 75(2), 109–120. https://doi.org/10.1016/j.jdermsci.2014.04.015","ieee":"M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, and D. Hendig, “Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum,” Journal of Dermatological Science, vol. 75, no. 2, pp. 109–120, 2014, doi: 10.1016/j.jdermsci.2014.04.015."},"page":"109-120","user_id":"83781","_id":"12984","keyword":["Pseudoxanthoma elasticum","Calcification","Pyrophosphate","Bisphosphonate","ABCC6","Tissue nonspecific alkaline phosphate","Ectonucleotide pyrophosphatase 1"]},{"publication_identifier":{"eissn":["1873-2933"],"issn":["0009-9120"]},"date_created":"2025-06-17T06:53:30Z","volume":47,"publication_status":"published","date_updated":"2025-06-17T13:48:52Z","abstract":[{"text":"Objectives\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder characterized by progressive calcification and fragmentation of elastic fibers. Because of the great clinical variability between PXE patients the involvement of modifier genes was recently suggested. Therefore, we investigated the association of single nucleotide variants (SNVs) in selected candidate genes known to regulate cellular pyrophosphate metabolism.\r\nDesign and methods\r\nWe used RLFP analyses to evaluate the distribution of SNVs in alkaline phosphatase (ALP), ectonucleotide pyrophosphatase 1 (ENPP1) and ankylosis (ANKH) in DNA samples from 190 German PXE patients and 190 age- and sex-matched healthy controls. Statistical analyses were performed using Fisher exact test and Bonferroni correction.\r\nResults\r\nThe screening revealed three different SNVs in three genes, which were associated with PXE. The SNV c.1190-65C > A (rs1780329, minor allele frequency (MAF) patients: 0.17; controls: 0.11; P = 0.04) in the ALP gene was significantly more frequent in PXE patients. Furthermore, PXE was highly associated with ANKH p.A98A genotype TT (P = 0.0012), although the MAF was not different between patients and controls. After correction for multiple testing according to the Bonferroni method, one SNV in the ENPP1 gene (c.313 + 9G > T, rs7773477) remained significantly associated with PXE with significantly higher MAF values in the patient cohort (MAF: 0.04 vs. 0.00; P = 0.0024) and a high association with PXE susceptibility (OR 27.96).\r\nConclusion\r\nPolymorphisms in ALP, ENPP1 and ANKH are important genetic risk factors contributing to PXE.","lang":"eng"}],"year":"2014","department":[{"_id":"DEP4010"}],"type":"scientific_journal_article","place":"Amsterdam","author":[{"full_name":"Dabisch-Ruthe, Mareike","id":"66516","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe","first_name":"Mareike"},{"full_name":"Brock, Alexander","last_name":"Brock","first_name":"Alexander"},{"first_name":"Patricia","last_name":"Kuzaj","full_name":"Kuzaj, Patricia"},{"first_name":"Peter","last_name":"Charbel Issa","full_name":"Charbel Issa, Peter"},{"last_name":"Szliska","full_name":"Szliska, Christiane","first_name":"Christiane"},{"first_name":"Cornelius","last_name":"Knabbe","full_name":"Knabbe, Cornelius"},{"first_name":"Doris","last_name":"Hendig","full_name":"Hendig, Doris"}],"issue":"15","title":"Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum","doi":"10.1016/j.clinbiochem.2014.07.003","publication":"Clinical Biochemistry","intvolume":" 47","citation":{"ieee":"M. Dabisch-Ruthe et al., “Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum,” Clinical Biochemistry, vol. 47, no. 15, pp. 60–67, 2014, doi: 10.1016/j.clinbiochem.2014.07.003.","apa":"Dabisch-Ruthe, M., Brock, A., Kuzaj, P., Charbel Issa, P., Szliska, C., Knabbe, C., & Hendig, D. (2014). Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. Clinical Biochemistry, 47(15), 60–67. https://doi.org/10.1016/j.clinbiochem.2014.07.003","chicago-de":"Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel Issa, Christiane Szliska, Cornelius Knabbe und Doris Hendig. 2014. Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. Clinical Biochemistry 47, Nr. 15: 60–67. doi:10.1016/j.clinbiochem.2014.07.003, .","havard":"M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe, D. Hendig, Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum, Clinical Biochemistry. 47 (2014) 60–67.","mla":"Dabisch-Ruthe, Mareike, et al. “Variants in Genes Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum.” Clinical Biochemistry, vol. 47, no. 15, 2014, pp. 60–67, https://doi.org/10.1016/j.clinbiochem.2014.07.003.","chicago":"Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel Issa, Christiane Szliska, Cornelius Knabbe, and Doris Hendig. “Variants in Genes Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum.” Clinical Biochemistry 47, no. 15 (2014): 60–67. https://doi.org/10.1016/j.clinbiochem.2014.07.003.","short":"M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe, D. Hendig, Clinical Biochemistry 47 (2014) 60–67.","ufg":"Dabisch-Ruthe, Mareike u. a.: Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum, in: Clinical Biochemistry 47 (2014), H. 15, S. 60–67.","din1505-2-1":"Dabisch-Ruthe, Mareike ; Brock, Alexander ; Kuzaj, Patricia ; Charbel Issa, Peter ; Szliska, Christiane ; Knabbe, Cornelius ; Hendig, Doris: Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. In: Clinical Biochemistry Bd. 47. Amsterdam, Elsevier (2014), Nr. 15, S. 60–67","bjps":"Dabisch-Ruthe M et al. (2014) Variants in Genes Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum. Clinical Biochemistry 47, 60–67.","van":"Dabisch-Ruthe M, Brock A, Kuzaj P, Charbel Issa P, Szliska C, Knabbe C, et al. Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. Clinical Biochemistry. 2014;47(15):60–7.","ama":"Dabisch-Ruthe M, Brock A, Kuzaj P, et al. Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. Clinical Biochemistry. 2014;47(15):60-67. doi:10.1016/j.clinbiochem.2014.07.003"},"language":[{"iso":"eng"}],"publisher":"Elsevier","user_id":"83781","_id":"12985","keyword":["Pseudoxanthoma elasticum","Calcification","Tissue nonspecific alkaline phosphatase","Ectonucleotide pyrophosphatase 1","Ankylosis"],"page":"60-67","status":"public"},{"status":"public","article_number":"118","intvolume":" 13","doi":"10.1186/1476-511x-13-118","publication":"Lipids in Health and Disease","user_id":"83781","_id":"12986","keyword":["Pseudoxanthoma elasticum","ABC transporter","ABCC6","Cholesterol biosynthesis","Atherosclerosis","HMG CoA reductase","SREBP2","PCSK9","LDLR","APOE"],"publisher":"Biomed Central ","language":[{"iso":"eng"}],"citation":{"short":"P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D. Hendig, Lipids in Health and Disease 13 (2014).","ufg":"Kuzaj, Patricia u. a.: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?, in: Lipids in Health and Disease 13 (2014), H. 1.","ieee":"P. Kuzaj et al., “ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?,” Lipids in Health and Disease, vol. 13, no. 1, Art. no. 118, 2014, doi: 10.1186/1476-511x-13-118.","apa":"Kuzaj, P., Kuhn, J., Dabisch-Ruthe, M., Faust, I., Götting, C., Knabbe, C., & Hendig, D. (2014). ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? Lipids in Health and Disease, 13(1), Article 118. https://doi.org/10.1186/1476-511x-13-118","chicago-de":"Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust, Christian Götting, Cornelius Knabbe und Doris Hendig. 2014. ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? Lipids in Health and Disease 13, Nr. 1. doi:10.1186/1476-511x-13-118, .","havard":"P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D. Hendig, ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?, Lipids in Health and Disease. 13 (2014).","din1505-2-1":"Kuzaj, Patricia ; Kuhn, Joachim ; Dabisch-Ruthe, Mareike ; Faust, Isabel ; Götting, Christian ; Knabbe, Cornelius ; Hendig, Doris: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? In: Lipids in Health and Disease Bd. 13. London, Biomed Central (2014), Nr. 1","bjps":"Kuzaj P et al. (2014) ABCC6- a New Player in Cellular Cholesterol and Lipoprotein Metabolism? Lipids in Health and Disease 13.","van":"Kuzaj P, Kuhn J, Dabisch-Ruthe M, Faust I, Götting C, Knabbe C, et al. ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? Lipids in Health and Disease. 2014;13(1).","ama":"Kuzaj P, Kuhn J, Dabisch-Ruthe M, et al. ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? Lipids in Health and Disease. 2014;13(1). doi:10.1186/1476-511x-13-118","mla":"Kuzaj, Patricia, et al. “ABCC6- a New Player in Cellular Cholesterol and Lipoprotein Metabolism?” Lipids in Health and Disease, vol. 13, no. 1, 118, 2014, https://doi.org/10.1186/1476-511x-13-118.","chicago":"Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust, Christian Götting, Cornelius Knabbe, and Doris Hendig. “ABCC6- a New Player in Cellular Cholesterol and Lipoprotein Metabolism?” Lipids in Health and Disease 13, no. 1 (2014). https://doi.org/10.1186/1476-511x-13-118."},"department":[{"_id":"DEP4010"}],"year":"2014","abstract":[{"text":"Background\r\nDysregulations in cholesterol and lipid metabolism have been linked to human diseases like hypercholesterolemia, atherosclerosis or the metabolic syndrome. Many ABC transporters are involved in trafficking of metabolites derived from these pathways. Pseudoxanthoma elasticum (PXE), an autosomal-recessive disease caused by ABCC6 mutations, is characterized by atherogenesis and soft tissue calcification.\r\nMethods\r\nIn this study we investigated the regulation of cholesterol biosynthesis in human dermal fibroblasts from PXE patients and healthy controls.\r\nResults\r\nGene expression analysis of 84 targets indicated dysregulations in cholesterol metabolism in PXE fibroblasts. Transcript levels of ABCC6 were strongly increased in lipoprotein-deficient serum (LPDS) and under serum starvation in healthy controls. For the first time, increased HMG CoA reductase activities were found in PXE fibroblasts. We further observed strongly elevated transcript and protein levels for the proprotein convertase subtilisin/kexin type 9 (PCSK9), as well as a significant reduction in APOE mRNA expression in PXE.\r\nConclusion\r\nIncreased cholesterol biosynthesis, elevated PCSK9 levels and reduced APOE mRNA expression newly found in PXE fibroblasts could enforce atherogenesis and cardiovascular risk in PXE patients. Moreover, the increase in ABCC6 expression accompanied by the induction of cholesterol biosynthesis supposes a functional role for ABCC6 in human lipoprotein and cholesterol homeostasis.","lang":"eng"}],"issue":"1","title":"ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?","author":[{"last_name":"Kuzaj","full_name":"Kuzaj, Patricia","first_name":"Patricia"},{"last_name":"Kuhn","full_name":"Kuhn, Joachim","first_name":"Joachim"},{"first_name":"Mareike","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe","full_name":"Dabisch-Ruthe, Mareike","id":"66516"},{"full_name":"Faust, Isabel","last_name":"Faust","first_name":"Isabel"},{"first_name":"Christian","full_name":"Götting, Christian","last_name":"Götting"},{"first_name":"Cornelius","full_name":"Knabbe, Cornelius","last_name":"Knabbe"},{"first_name":"Doris","last_name":"Hendig","full_name":"Hendig, Doris"}],"place":"London","type":"scientific_journal_article","date_created":"2025-06-17T06:53:58Z","publication_identifier":{"eissn":["1476-511X"]},"quality_controlled":"1","date_updated":"2025-06-17T13:49:15Z","volume":13,"publication_status":"published"},{"date_created":"2025-06-17T06:54:30Z","publication_identifier":{"eissn":["1932-6203"]},"quality_controlled":"1","date_updated":"2025-06-17T13:49:26Z","volume":9,"publication_status":"published","department":[{"_id":"DEP4010"}],"year":"2014","abstract":[{"text":"Mutations in the ABC transporter ABCC6 were recently identified as cause of Pseudoxanthoma elasticum (PXE), a rare genetic disorder characterized by progressive mineralization of elastic fibers. We used an untargeted metabolic approach to identify biochemical differences between human dermal fibroblasts from healthy controls and PXE patients in an attempt to find a link between ABCC6 deficiency, cellular metabolic alterations and disease pathogenesis. 358 compounds were identified by mass spectrometry covering lipids, amino acids, peptides, carbohydrates, nucleotides, vitamins and cofactors, xenobiotics and energy metabolites. We found substantial differences in glycerophospholipid composition, leucine dipeptides, and polypeptides as well as alterations in pantothenate and guanine metabolism to be significantly associated with PXE pathogenesis. These findings can be linked to extracellular matrix remodeling and increased oxidative stress, which reflect characteristic hallmarks of PXE. Our study could facilitate a better understanding of biochemical pathways involved in soft tissue mineralization.","lang":"eng"}],"title":"Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls","issue":"9","author":[{"last_name":"Kuzaj","full_name":"Kuzaj, Patricia","first_name":"Patricia"},{"first_name":"Joachim","last_name":"Kuhn","full_name":"Kuhn, Joachim"},{"last_name":"Michalek","full_name":"Michalek, Ryan D.","first_name":"Ryan D."},{"full_name":"Karoly, Edward D.","last_name":"Karoly","first_name":"Edward D."},{"last_name":"Faust","full_name":"Faust, Isabel","first_name":"Isabel"},{"first_name":"Mareike","last_name":"Dabisch-Ruthe","orcid":"https://orcid.org/0009-0008-7644-0826","full_name":"Dabisch-Ruthe, Mareike","id":"66516"},{"first_name":"Cornelius","full_name":"Knabbe, Cornelius","last_name":"Knabbe"},{"first_name":"Doris","last_name":"Hendig","full_name":"Hendig, Doris"}],"place":"San Francisco, California, US","type":"scientific_journal_article","intvolume":" 9","doi":"10.1371/journal.pone.0108336","publication":"PLOS ONE / Public Library of Science ","user_id":"83781","_id":"12987","publisher":"Public Library of Science (PLoS)","language":[{"iso":"eng"}],"citation":{"short":"P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe, C. Knabbe, D. Hendig, PLOS ONE / Public Library of Science 9 (2014).","ufg":"Kuzaj, Patricia u. a.: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls, in: PLOS ONE / Public Library of Science 9 (2014), H. 9.","din1505-2-1":"Kuzaj, Patricia ; Kuhn, Joachim ; Michalek, Ryan D. ; Karoly, Edward D. ; Faust, Isabel ; Dabisch-Ruthe, Mareike ; Knabbe, Cornelius ; Hendig, Doris: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. In: PLOS ONE / Public Library of Science Bd. 9. San Francisco, California, US, Public Library of Science (PLoS) (2014), Nr. 9","bjps":"Kuzaj P et al. (2014) Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. PLOS ONE / Public Library of Science 9.","van":"Kuzaj P, Kuhn J, Michalek RD, Karoly ED, Faust I, Dabisch-Ruthe M, et al. Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. PLOS ONE / Public Library of Science . 2014;9(9).","ama":"Kuzaj P, Kuhn J, Michalek RD, et al. Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. PLOS ONE / Public Library of Science . 2014;9(9). doi:10.1371/journal.pone.0108336","ieee":"P. Kuzaj et al., “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls,” PLOS ONE / Public Library of Science , vol. 9, no. 9, Art. no. e108336, 2014, doi: 10.1371/journal.pone.0108336.","apa":"Kuzaj, P., Kuhn, J., Michalek, R. D., Karoly, E. D., Faust, I., Dabisch-Ruthe, M., Knabbe, C., & Hendig, D. (2014). Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. PLOS ONE / Public Library of Science , 9(9), Article e108336. https://doi.org/10.1371/journal.pone.0108336","havard":"P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe, C. Knabbe, D. Hendig, Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls, PLOS ONE / Public Library of Science . 9 (2014).","chicago-de":"Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe und Doris Hendig. 2014. Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. PLOS ONE / Public Library of Science 9, Nr. 9. doi:10.1371/journal.pone.0108336, .","mla":"Kuzaj, Patricia, et al. “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls.” PLOS ONE / Public Library of Science , vol. 9, no. 9, e108336, 2014, https://doi.org/10.1371/journal.pone.0108336.","chicago":"Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe, and Doris Hendig. “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls.” PLOS ONE / Public Library of Science 9, no. 9 (2014). https://doi.org/10.1371/journal.pone.0108336."},"status":"public","article_number":"e108336"},{"type":"conference","title":"Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food","author":[{"first_name":"Knut","last_name":"Schwarzer","id":"13329","full_name":"Schwarzer, Knut"},{"id":"13209","full_name":"Schneider, Jan","orcid":"0000-0001-6401-8873","last_name":"Schneider","first_name":"Jan"},{"id":"12640","full_name":"Becker, Barbara","last_name":"Becker","first_name":"Barbara"},{"first_name":"Ulrich","id":"12119","full_name":"Müller, Ulrich","last_name":"Müller"}],"status":"public","conference":{"location":"Stuttgart - Hohenheim","name":"ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik ","start_date":"2012-03-19","end_date":"2012-03-21"},"year":"2012","department":[{"_id":"DEP4009"},{"_id":"DEP4023"},{"_id":"DEP4010"},{"_id":"DEP4018"}],"language":[{"iso":"ger"}],"citation":{"chicago":"Schwarzer, Knut, Jan Schneider, Barbara Becker, and Ulrich Müller. “Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food,” 2012.","mla":"Schwarzer, Knut, et al. Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. 2012.","havard":"K. Schwarzer, J. Schneider, B. Becker, U. Müller, Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food, in: 2012.","chicago-de":"Schwarzer, Knut, Jan Schneider, Barbara Becker und Ulrich Müller. 2012. Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. In: .","apa":"Schwarzer, K., Schneider, J., Becker, B., & Müller, U. (2012). Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik , Stuttgart - Hohenheim.","ieee":"K. Schwarzer, J. Schneider, B. Becker, and U. Müller, “Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food,” presented at the ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik , Stuttgart - Hohenheim, 2012.","ama":"Schwarzer K, Schneider J, Becker B, Müller U. Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. In: ; 2012.","van":"Schwarzer K, Schneider J, Becker B, Müller U. Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. In 2012.","bjps":"Schwarzer K et al. (2012) Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food.","din1505-2-1":"Schwarzer, Knut ; Schneider, Jan ; Becker, Barbara ; Müller, Ulrich: Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. In: , 2012","ufg":"Schwarzer, Knut u. a.: Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food, in: o. Hg.: o. O. 2012.","short":"K. Schwarzer, J. Schneider, B. Becker, U. Müller, in: 2012."},"date_updated":"2024-05-24T06:46:24Z","_id":"5348","user_id":"83779","date_created":"2021-03-30T07:24:31Z"},{"date_updated":"2025-06-17T13:49:03Z","volume":12,"publication_status":"published","quality_controlled":"1","date_created":"2025-06-17T06:52:13Z","publication_identifier":{"eissn":["1471-2334"]},"type":"scientific_journal_article","place":"Berlin ; Heidelberg","author":[{"first_name":"Mareike","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe","full_name":"Dabisch-Ruthe, Mareike","id":"66516"},{"first_name":"Tanja","last_name":"Vollmer","full_name":"Vollmer, Tanja"},{"first_name":"Ortwin","last_name":"Adams","full_name":"Adams, Ortwin"},{"full_name":"Knabbe, Cornelius","last_name":"Knabbe","first_name":"Cornelius"},{"first_name":"Jens","full_name":"Dreier, Jens","last_name":"Dreier"}],"issue":"1","title":"Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay","year":"2012","abstract":[{"text":"Background\r\nA broad spectrum of pathogens is causative for respiratory tract infections, but symptoms are mostly similar. Therefore, the identification of the causative viruses and bacteria is only feasible using multiplex PCR or several monoplex PCR tests in parallel.\r\nMethods\r\nThe analytical sensitivity of three multiplex PCR assays, RespiFinder-19, RespiFinder-SMART-22 and xTAG-Respiratory-Virus-Panel-Fast-Assay (RVP), were compared to monoplex real-time PCR with quantified standardized control material. All assays include the most common respiratory pathogens.\r\nResults\r\nTo compare the analytical sensitivity of the multiplex assays, samples were inoculated with 13 different quantified viruses in the range of 101 to 105 copies/ml. Concordant results were received for rhinovirus, whereas the RVP detected influenzavirus, RSV and hMPV more frequently in low concentrations. The RespiFinder-19 and the RespiFinder-SMART-22 showed a higher analytical sensitivity for adenoviruses and coronaviruses, whereas the RVP was incapable to detect adenovirus and coronavirus in concentrations of 104 copies/ml. The RespiFinder-19 and RespiFinder-SMART-22A did not detect influenzaviruses (104 copies/ml) and RSV (103 copies/ml). The detection of all 13 viruses in one sample was only achieved using monoplex PCR. To analyze possible competitive amplification reactions between the different viruses, samples were further inoculated with only 4 different viruses in one sample. Compared to the detection of 13 viruses in parallel, only a few differences were found.\r\nThe incidence of respiratory viruses was compared in tracheal secretion (TS) samples (n = 100) of mechanically ventilated patients in winter (n = 50) and summer (n = 50). In winter, respiratory viruses were detected in 32 TS samples (64%) by RespiFinder-19, whereas the detection rate with RVP was only 22%. The most frequent viruses were adenovirus (32%) and PIV-2 (20%). Multiple infections were detected in 16 TS samples (32%) by RespiFinder-19. Fewer infections were found in summer (RespiFinder-19: 20%; RVP: 6%). All positive results were verified using monoplex PCR.\r\nConclusions\r\nMultiplex PCR tests have a broad spectrum of pathogens to test at a time. Analysis of multiple inoculated samples revealed a different focus of the detected virus types by the three assays. Analysis of clinical samples showed a high concordance of detected viruses by the RespiFinder-19 compared to monoplex tests.","lang":"eng"}],"department":[{"_id":"DEP4010"}],"publisher":"Springer","citation":{"ufg":"Dabisch-Ruthe, Mareike u. a.: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, in: BMC Infectious Diseases 12 (2012), H. 1.","short":"M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, BMC Infectious Diseases 12 (2012).","din1505-2-1":"Dabisch-Ruthe, Mareike ; Vollmer, Tanja ; Adams, Ortwin ; Knabbe, Cornelius ; Dreier, Jens: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. In: BMC Infectious Diseases Bd. 12. Berlin ; Heidelberg, Springer (2012), Nr. 1","bjps":"Dabisch-Ruthe M et al. (2012) Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay. BMC Infectious Diseases 12.","van":"Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infectious Diseases. 2012;12(1).","ama":"Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infectious Diseases. 2012;12(1). doi:10.1186/1471-2334-12-163","ieee":"M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, and J. Dreier, “Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay,” BMC Infectious Diseases, vol. 12, no. 1, Art. no. 163, 2012, doi: 10.1186/1471-2334-12-163.","havard":"M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, BMC Infectious Diseases. 12 (2012).","chicago-de":"Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe und Jens Dreier. 2012. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infectious Diseases 12, Nr. 1. doi:10.1186/1471-2334-12-163, .","apa":"Dabisch-Ruthe, M., Vollmer, T., Adams, O., Knabbe, C., & Dreier, J. (2012). Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infectious Diseases, 12(1), Article 163. https://doi.org/10.1186/1471-2334-12-163","chicago":"Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe, and Jens Dreier. “Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” BMC Infectious Diseases 12, no. 1 (2012). https://doi.org/10.1186/1471-2334-12-163.","mla":"Dabisch-Ruthe, Mareike, et al. “Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” BMC Infectious Diseases, vol. 12, no. 1, 163, 2012, https://doi.org/10.1186/1471-2334-12-163."},"language":[{"iso":"eng"}],"_id":"12983","keyword":["Respiratory Virus","Elution Volume","Multiplex Assay","Multiple Infection","Bocavirus"],"user_id":"83781","publication":"BMC Infectious Diseases","doi":"10.1186/1471-2334-12-163","intvolume":" 12","status":"public","article_number":"163"},{"department":[{"_id":"DEP4010"},{"_id":"DEP4009"},{"_id":"DEP4018"}],"year":2010,"issue":"5","title":"Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim","status":"public","author":[{"orcid":"0000-0001-6401-8873","last_name":"Schneider","id":"13209","full_name":"Schneider, Jan","first_name":"Jan"},{"first_name":"Ulrich","last_name":"Müller","full_name":"Müller, Ulrich","id":"12119"},{"id":"13329","full_name":"Schwarzer, Knut","last_name":"Schwarzer","first_name":"Knut"},{"id":"12640","full_name":"Becker, Barbara","last_name":"Becker","first_name":"Barbara"},{"first_name":"Patrick","last_name":"Wilhelm","full_name":"Wilhelm, Patrick","id":"12013"}],"type":"journal_article","date_created":"2020-06-02T10:24:20Z","publication_identifier":{"issn":["0934-9340"]},"publication":"Brauwelt International","page":"252 - 256","date_updated":"2023-03-15T13:49:42Z","user_id":"12013","_id":"2748","publication_status":"published","publisher":"Fachverlag Hans Carl GmbH","language":[{"iso":"eng"}],"citation":{"short":"J. Schneider, U. Müller, K. Schwarzer, B. Becker, P. Wilhelm, Brauwelt International (2010) 252–256.","ufg":"Schneider, Jan u. a.: Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim, in: Brauwelt International (2010), H. 5, S. 252–256.","din1505-2-1":"Schneider, Jan ; Müller, Ulrich ; Schwarzer, Knut ; Becker, Barbara ; Wilhelm, Patrick: Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. In: Brauwelt International, Fachverlag Hans Carl GmbH (2010), Nr. 5, S. 252–256","bjps":"Schneider J et al. (2010) Lemgo Database for D- and z-Values: Useful for Beverage Spoilage Microorgansim. Brauwelt International 252–256.","ama":"Schneider J, Müller U, Schwarzer K, Becker B, Wilhelm P. Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International. 2010;(5):252-256.","van":"Schneider J, Müller U, Schwarzer K, Becker B, Wilhelm P. Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International. 2010;(5):252–6.","ieee":"J. Schneider, U. Müller, K. Schwarzer, B. Becker, and P. Wilhelm, “Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim,” Brauwelt International, no. 5, pp. 252–256, 2010.","apa":"Schneider, J., Müller, U., Schwarzer, K., Becker, B., & Wilhelm, P. (2010). Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International, 5, 252–256.","havard":"J. Schneider, U. Müller, K. Schwarzer, B. Becker, P. Wilhelm, Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim, Brauwelt International. (2010) 252–256.","chicago-de":"Schneider, Jan, Ulrich Müller, Knut Schwarzer, Barbara Becker und Patrick Wilhelm. 2010. Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International, Nr. 5: 252–256.","mla":"Schneider, Jan, et al. “Lemgo Database for D- and z-Values: Useful for Beverage Spoilage Microorgansim.” Brauwelt International, no. 5, 2010, pp. 252–56.","chicago":"Schneider, Jan, Ulrich Müller, Knut Schwarzer, Barbara Becker, and Patrick Wilhelm. “Lemgo Database for D- and z-Values: Useful for Beverage Spoilage Microorgansim.” Brauwelt International, no. 5 (2010): 252–56."}},{"page":"540 - 544","date_updated":"2023-03-15T13:49:42Z","_id":"2763","publication_status":"published","volume":150,"user_id":"12013","publisher":"Fachverlag Hans Carl GmbH","citation":{"short":"K. Schwarzer, J. Schneider, U. Müller, B. Becker, P. Wilhelm, Brauwelt 150 (2010) 540–544.","ufg":"Schwarzer, Knut u. a.: Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen, in: Brauwelt 150 (2010), H. 18, S. 540–544.","din1505-2-1":"Schwarzer, Knut ; Schneider, Jan ; Müller, Ulrich ; Becker, Barbara ; Wilhelm, Patrick: Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. In: Brauwelt Bd. 150, Fachverlag Hans Carl GmbH (2010), Nr. 18, S. 540–544","bjps":"Schwarzer K et al. (2010) Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für Getränkeschädliche Mikroorganismen. Brauwelt 150, 540–544.","van":"Schwarzer K, Schneider J, Müller U, Becker B, Wilhelm P. Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt. 2010;150(18):540–4.","ama":"Schwarzer K, Schneider J, Müller U, Becker B, Wilhelm P. Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt. 2010;150(18):540-544.","ieee":"K. Schwarzer, J. Schneider, U. Müller, B. Becker, and P. Wilhelm, “Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen,” Brauwelt, vol. 150, no. 18, pp. 540–544, 2010.","apa":"Schwarzer, K., Schneider, J., Müller, U., Becker, B., & Wilhelm, P. (2010). Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt, 150(18), 540–544.","chicago-de":"Schwarzer, Knut, Jan Schneider, Ulrich Müller, Barbara Becker und Patrick Wilhelm. 2010. Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt 150, Nr. 18: 540–544.","havard":"K. Schwarzer, J. Schneider, U. Müller, B. Becker, P. Wilhelm, Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen, Brauwelt. 150 (2010) 540–544.","mla":"Schwarzer, Knut, et al. “Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für Getränkeschädliche Mikroorganismen.” Brauwelt, vol. 150, no. 18, 2010, pp. 540–44.","chicago":"Schwarzer, Knut, Jan Schneider, Ulrich Müller, Barbara Becker, and Patrick Wilhelm. “Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für Getränkeschädliche Mikroorganismen.” Brauwelt 150, no. 18 (2010): 540–44."},"language":[{"iso":"eng"}],"date_created":"2020-06-03T07:13:41Z","publication":"Brauwelt","publication_identifier":{"issn":["\t0724-696X"]},"intvolume":" 150","author":[{"first_name":"Knut","last_name":"Schwarzer","full_name":"Schwarzer, Knut","id":"13329"},{"first_name":"Jan","last_name":"Schneider","orcid":"0000-0001-6401-8873","id":"13209","full_name":"Schneider, Jan"},{"id":"12119","full_name":"Müller, Ulrich","last_name":"Müller","first_name":"Ulrich"},{"full_name":"Becker, Barbara","id":"12640","last_name":"Becker","first_name":"Barbara"},{"first_name":"Patrick","full_name":"Wilhelm, Patrick","id":"12013","last_name":"Wilhelm"}],"status":"public","title":"Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen","issue":"18","type":"journal_article","department":[{"_id":"DEP4010"},{"_id":"DEP4009"},{"_id":"DEP4018"}],"year":2010},{"type":"newspaper_article","status":"public","author":[{"first_name":"Barbara","last_name":"Becker","full_name":"Becker, Barbara","id":"12640"},{"first_name":"Goetz","last_name":"Hildebrandt","full_name":"Hildebrandt, Goetz"},{"full_name":"Erol, Irfan ","last_name":"Erol","first_name":"Irfan "},{"first_name":"Josef","last_name":"Kleer","full_name":"Kleer, Josef"},{"last_name":"Manopas","full_name":"Manopas, Aranya Sira","first_name":"Aranya Sira"}],"title":"Von der Döner - Prüfung zum Döner - Heariing","issue":"7","year":2009,"department":[{"_id":"DEP4010"}],"citation":{"short":"B. Becker, G. Hildebrandt, I. Erol, J. Kleer, A.S. Manopas, Fleischwirtschaft (2009) 61–65.","ufg":"Becker, Barbara et. al. (2009): Von der Döner - Prüfung zum Döner - Heariing, in: Fleischwirtschaft (7) (2009), S. 61–65.","din1505-2-1":"Becker, Barbara ; Hildebrandt, Goetz ; Erol, Irfan ; Kleer, Josef ; Manopas, Aranya Sira: Von der Döner - Prüfung zum Döner - Heariing. In: Fleischwirtschaft (2009), Nr. 7","bjps":"Becker B et al. (2009) Von Der Döner - Prüfung Zum Döner - Heariing. Fleischwirtschaft , 61–65.","van":"Becker B, Hildebrandt G, Erol I, Kleer J, Manopas AS. Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft . 2009;61–5.","ama":"Becker B, Hildebrandt G, Erol I, Kleer J, Manopas AS. Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft . 2009:61-65.","ieee":"B. Becker, G. Hildebrandt, I. Erol, J. Kleer, and A. S. Manopas, “Von der Döner - Prüfung zum Döner - Heariing,” Fleischwirtschaft , no. 7, pp. 61–65, 2009.","apa":"Becker, B., Hildebrandt, G., Erol, I., Kleer, J., & Manopas, A. S. (2009). Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft , pp. 61–65.","havard":"B. Becker, G. Hildebrandt, I. Erol, J. Kleer, A.S. Manopas, Von der Döner - Prüfung zum Döner - Heariing, Fleischwirtschaft . (2009) 61–65.","chicago-de":"Becker, Barbara, Goetz Hildebrandt, Irfan Erol, Josef Kleer und Aranya Sira Manopas. 2009. Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft .","mla":"Becker, Barbara, et al. “Von Der Döner - Prüfung Zum Döner - Heariing.” Fleischwirtschaft , no. 7, 2009, pp. 61–65.","chicago":"Becker, Barbara, Goetz Hildebrandt, Irfan Erol, Josef Kleer, and Aranya Sira Manopas. “Von Der Döner - Prüfung Zum Döner - Heariing.” Fleischwirtschaft , 2009."},"language":[{"iso":"eng"}],"_id":"2361","user_id":"74222","publication_date":"19.03.2019","page":"61-65","date_updated":"2023-03-15T13:49:39Z","publication":"Fleischwirtschaft ","date_created":"2020-05-18T09:40:45Z"},{"year":"2009","department":[{"_id":"DEP4023"},{"_id":"DEP4009"},{"_id":"DEP4010"},{"_id":"DEP4018"}],"type":"conference_abstract","author":[{"last_name":"Schwarzer","id":"13329","full_name":"Schwarzer, Knut","first_name":"Knut"},{"last_name":"Becker","full_name":"Becker, Barbara","id":"12640","first_name":"Barbara"},{"first_name":"Jan","id":"13209","full_name":"Schneider, Jan","last_name":"Schneider","orcid":"0000-0001-6401-8873"},{"last_name":"Müller","id":"12119","full_name":"Müller, Ulrich","first_name":"Ulrich"}],"status":"public","title":"Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung","conference":{"name":"ProcessNet Fachausschuss Lebensmittelverfahrenstechnik","location":"Lausanne","start_date":"2009-03-23","end_date":"2009-03-25"},"date_created":"2021-09-06T10:43:56Z","citation":{"mla":"Schwarzer, Knut, et al. Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung. 2009.","chicago":"Schwarzer, Knut, Barbara Becker, Jan Schneider, and Ulrich Müller. “Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung,” 2009.","apa":"Schwarzer, K., Becker, B., Schneider, J., & Müller, U. (2009). Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. ProcessNet Fachausschuss Lebensmittelverfahrenstechnik, Lausanne.","chicago-de":"Schwarzer, Knut, Barbara Becker, Jan Schneider und Ulrich Müller. 2009. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: .","havard":"K. Schwarzer, B. Becker, J. Schneider, U. Müller, Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: 2009.","ieee":"K. Schwarzer, B. Becker, J. Schneider, and U. Müller, “Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung,” presented at the ProcessNet Fachausschuss Lebensmittelverfahrenstechnik, Lausanne, 2009.","van":"Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In 2009.","ama":"Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: ; 2009.","bjps":"Schwarzer K et al. (2009) Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung.","din1505-2-1":"Schwarzer, Knut ; Becker, Barbara ; Schneider, Jan ; Müller, Ulrich: Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: , 2009","short":"K. Schwarzer, B. Becker, J. Schneider, U. Müller, in: 2009.","ufg":"Schwarzer, Knut u. a.: Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: o. Hg.: o. O. 2009."},"language":[{"iso":"eng"}],"date_updated":"2024-05-23T11:12:12Z","user_id":"83779","_id":"6128"},{"language":[{"iso":"eng"}],"citation":{"ieee":"K. Schwarzer, B. Becker, J. Schneider, and U. Müller, “Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung,” presented at the GDL-Kongress , Lemgo, 2009.","ufg":"Schwarzer, Knut u. a.: Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: o. Hg.: o. O. 2009.","short":"K. Schwarzer, B. Becker, J. Schneider, U. Müller, in: 2009.","havard":"K. Schwarzer, B. Becker, J. Schneider, U. Müller, Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: 2009.","chicago-de":"Schwarzer, Knut, Barbara Becker, Jan Schneider und Ulrich Müller. 2009. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: .","din1505-2-1":"Schwarzer, Knut ; Becker, Barbara ; Schneider, Jan ; Müller, Ulrich: Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: , 2009","apa":"Schwarzer, K., Becker, B., Schneider, J., & Müller, U. (2009). Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. GDL-Kongress , Lemgo.","bjps":"Schwarzer K et al. (2009) Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung.","chicago":"Schwarzer, Knut, Barbara Becker, Jan Schneider, and Ulrich Müller. “Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung,” 2009.","van":"Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In 2009.","ama":"Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: ; 2009.","mla":"Schwarzer, Knut, et al. Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung. 2009."},"date_updated":"2024-05-23T11:12:44Z","user_id":"83779","_id":"6141","date_created":"2021-09-06T11:10:55Z","type":"conference_abstract","title":"Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung","author":[{"first_name":"Knut","full_name":"Schwarzer, Knut","id":"13329","last_name":"Schwarzer"},{"first_name":"Barbara","last_name":"Becker","id":"12640","full_name":"Becker, Barbara"},{"first_name":"Jan","full_name":"Schneider, Jan","id":"13209","orcid":"0000-0001-6401-8873","last_name":"Schneider"},{"first_name":"Ulrich","last_name":"Müller","id":"12119","full_name":"Müller, Ulrich"}],"status":"public","conference":{"start_date":"2009-10-22","end_date":"2009-10-24","location":"Lemgo","name":"GDL-Kongress "},"year":"2009","department":[{"_id":"DEP4023"},{"_id":"DEP4009"},{"_id":"DEP4010"},{"_id":"DEP4018"}]},{"quality_controlled":"1","date_created":"2025-06-17T06:26:59Z","publication_identifier":{"issn":["0099-2240 "],"eissn":["1098-5336"]},"date_updated":"2025-06-17T13:49:35Z","volume":76,"publication_status":"published","year":"2009","abstract":[{"lang":"eng","text":"Contaminated food is a significant vehicle for human norovirus transmission. The present study determined the effect of physicochemical treatments on the tenacity of infective human norovirus genogroup II in selected foods. Artificially contaminated produce was subjected to a number of processes used by the food industry for preservation and by the consumer for storage and preparation. Virus recovery was carried out by using ultrafiltration and was monitored by using bacteriophage MS2 as an internal process control. Norovirus was quantified by using monoplex one-step TaqMan real-time reverse transcription (RT)-PCR and an external standard curve based on recombinant RNA standards. An RNase pretreatment step was used to avoid false-positive PCR results caused by accessible RNA, which allowed detection of intact virus particles. Significant reductions in titers were obtained with heat treatments usually applied by consumers for food preparation (baking, cooking, roasting). Generally, processes used for preservation and storage, such as cooling, freezing, acidification (≥pH 4.5), and moderate heat treatments (pasteurization), appear to be insufficient to inactivate norovirus within a food matrix or on the surface of food. Besides data for persistence in processed food, comparable data for individual matrix-specific protective effects, recovery rates, and inhibitory effects on the PCRs were obtained in this study. The established procedure might be used for other noncultivable enteric RNA viruses that are connected to food-borne diseases. The data obtained in this study may also help optimize the process for inactivation of norovirus in food by adjusting food processing technologies and may promote the development of risk assessment systems in order to improve consumer protection."}],"department":[{"_id":"DEP4010"}],"type":"scientific_journal_article","place":"Washington, DC [u.a.]","author":[{"full_name":"Mormann, Sascha","last_name":"Mormann","first_name":"Sascha"},{"full_name":"Dabisch-Ruthe, Mareike","id":"66516","last_name":"Dabisch-Ruthe","orcid":"https://orcid.org/0009-0008-7644-0826","first_name":"Mareike"},{"first_name":"Barbara","full_name":"Becker, Barbara","id":"12640","last_name":"Becker"}],"issue":"2","title":"Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods","doi":"10.1128/aem.01797-09","publication":" Applied and environmental microbiology / American Society for Microbiology","intvolume":" 76","publisher":"American Society for Microbiology","citation":{"short":"S. Mormann, M. Dabisch-Ruthe, B. Becker, Applied and Environmental Microbiology / American Society for Microbiology 76 (2009) 536–545.","ieee":"S. Mormann, M. Dabisch-Ruthe, and B. Becker, “Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods,” Applied and environmental microbiology / American Society for Microbiology, vol. 76, no. 2, pp. 536–545, 2009, doi: 10.1128/aem.01797-09.","ufg":"Mormann, Sascha/Dabisch-Ruthe, Mareike/Becker, Barbara: Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods, in: Applied and environmental microbiology / American Society for Microbiology 76 (2009), H. 2, S. 536–545.","apa":"Mormann, S., Dabisch-Ruthe, M., & Becker, B. (2009). Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. Applied and Environmental Microbiology / American Society for Microbiology, 76(2), 536–545. https://doi.org/10.1128/aem.01797-09","chicago-de":"Mormann, Sascha, Mareike Dabisch-Ruthe und Barbara Becker. 2009. Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. Applied and environmental microbiology / American Society for Microbiology 76, Nr. 2: 536–545. doi:10.1128/aem.01797-09, .","havard":"S. Mormann, M. Dabisch-Ruthe, B. Becker, Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods, Applied and Environmental Microbiology / American Society for Microbiology. 76 (2009) 536–545.","din1505-2-1":"Mormann, Sascha ; Dabisch-Ruthe, Mareike ; Becker, Barbara: Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. In: Applied and environmental microbiology / American Society for Microbiology Bd. 76. Washington, DC [u.a.], American Society for Microbiology (2009), Nr. 2, S. 536–545","bjps":"Mormann S, Dabisch-Ruthe M and Becker B (2009) Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. Applied and environmental microbiology / American Society for Microbiology 76, 536–545.","van":"Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. Applied and environmental microbiology / American Society for Microbiology. 2009;76(2):536–45.","mla":"Mormann, Sascha, et al. “Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods.” Applied and Environmental Microbiology / American Society for Microbiology, vol. 76, no. 2, 2009, pp. 536–45, https://doi.org/10.1128/aem.01797-09.","ama":"Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. Applied and environmental microbiology / American Society for Microbiology. 2009;76(2):536-545. doi:10.1128/aem.01797-09","chicago":"Mormann, Sascha, Mareike Dabisch-Ruthe, and Barbara Becker. “Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods.” Applied and Environmental Microbiology / American Society for Microbiology 76, no. 2 (2009): 536–45. https://doi.org/10.1128/aem.01797-09."},"language":[{"iso":"eng"}],"page":"536-545","user_id":"83781","_id":"12982","status":"public"},{"citation":{"ieee":"B. Becker, “Lebensmittelassoziierte Viren - aktuelle Apekte,” presented at the Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie, 2004.","ufg":"Becker, Barbara (2004): Lebensmittelassoziierte Viren - aktuelle Apekte, in: .","short":"B. Becker, in: 2004.","chicago-de":"Becker, Barbara. 2004. Lebensmittelassoziierte Viren - aktuelle Apekte. In: .","havard":"B. Becker, Lebensmittelassoziierte Viren - aktuelle Apekte, in: 2004.","din1505-2-1":"Becker, Barbara: Lebensmittelassoziierte Viren - aktuelle Apekte. In: , 2004","apa":"Becker, B. (2004). Lebensmittelassoziierte Viren - aktuelle Apekte. Presented at the Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie.","bjps":"Becker B (2004) Lebensmittelassoziierte Viren - Aktuelle Apekte.","chicago":"Becker, Barbara. “Lebensmittelassoziierte Viren - Aktuelle Apekte,” 2004.","van":"Becker B. Lebensmittelassoziierte Viren - aktuelle Apekte. In 2004.","mla":"Becker, Barbara. Lebensmittelassoziierte Viren - Aktuelle Apekte. 2004.","ama":"Becker B. Lebensmittelassoziierte Viren - aktuelle Apekte. In: ; 2004."},"language":[{"iso":"eng"}],"date_updated":"2023-03-15T13:49:39Z","_id":"2359","user_id":"74222","date_created":"2020-05-18T09:33:00Z","type":"conference","status":"public","author":[{"first_name":"Barbara","id":"12640","full_name":"Becker, Barbara","last_name":"Becker"}],"title":"Lebensmittelassoziierte Viren - aktuelle Apekte","conference":{"end_date":"16.07.2004","start_date":"14.07.2004","name":"Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie"},"year":2004,"department":[{"_id":"DEP4010"}]}]