[{"publication":"Brauwelt International","publication_identifier":{"unknown":["0934-9340"]},"date_created":"2020-05-18T10:12:34Z","citation":{"short":"B. Becker, Brauwelt International (2014) 252–256.","ufg":"<b>Becker, Barbara (2014)</b>: useful for beverage spoilage microoganism, in: <i>Brauwelt International</i> (<i>5</i>) (<i>2014</i>), S. 252–256.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Becker, Barbara</span>: useful for beverage spoilage microoganism. In: <i>Brauwelt International</i> (2014), Nr. 5","bjps":"<b>Becker B</b> (2014) Useful for Beverage Spoilage Microoganism. <i>Brauwelt International</i>, 252–256.","van":"Becker B. useful for beverage spoilage microoganism. Brauwelt International. 2014;252–6.","ama":"Becker B. useful for beverage spoilage microoganism. <i>Brauwelt International</i>. 2014:252-256.","ieee":"B. Becker, “useful for beverage spoilage microoganism,” <i>Brauwelt International</i>, no. 5, pp. 252–256, 2014.","apa":"Becker, B. (2014). useful for beverage spoilage microoganism. <i>Brauwelt International</i>, pp. 252–256.","havard":"B. Becker, useful for beverage spoilage microoganism, Brauwelt International. (2014) 252–256.","chicago-de":"Becker, Barbara. 2014. useful for beverage spoilage microoganism. <i>Brauwelt International</i>.","mla":"Becker, Barbara. “Useful for Beverage Spoilage Microoganism.” <i>Brauwelt International</i>, no. 5, 2014, pp. 252–56.","chicago":"Becker, Barbara. “Useful for Beverage Spoilage Microoganism.” <i>Brauwelt International</i>, 2014."},"language":[{"iso":"eng"}],"_id":"2363","user_id":"74222","publication_date":"2014","date_updated":"2023-03-15T13:49:39Z","page":"252-256","year":2014,"department":[{"_id":"DEP4010"}],"type":"newspaper_article","status":"public","author":[{"last_name":"Becker","full_name":"Becker, Barbara","id":"12640","first_name":"Barbara"}],"title":"useful for beverage spoilage microoganism","issue":"5"},{"department":[{"_id":"DEP4010"}],"year":"2014","author":[{"first_name":"Barbara","id":"12640","full_name":"Becker, Barbara","last_name":"Becker"},{"first_name":"Verena","last_name":"Becker","full_name":"Becker, Verena"},{"full_name":"Nerenz, Heiko","last_name":"Nerenz","first_name":"Heiko"},{"first_name":"Andreas","last_name":"Schrader","full_name":"Schrader, Andreas"}],"title":"Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten","issue":"5","type":"journal_article","date_created":"2020-05-20T07:28:39Z","has_accepted_license":"1","publication_identifier":{"issn":["0942-7694"]},"date_updated":"2024-12-02T14:21:46Z","volume":140,"publication_status":"published","file":[{"file_id":"2405","file_size":3196553,"relation":"main_file","creator":"z5h-0dr","date_created":"2020-05-20T07:37:08Z","content_type":"application/pdf","access_level":"closed","file_name":"becker(2).pdf","date_updated":"2024-12-02T14:21:45Z"}],"ddc":["610","660"],"status":"public","file_date_updated":"2024-12-02T14:21:45Z","publication":"SOFW-Journal : Home & personal care ingredients & formulations; Deutsche Ausgabe","intvolume":"       140","page":"26 - 27, 30","user_id":"83781","_id":"2403","publisher":"Verl. für Chemische Industrie, Ziolkowsky","citation":{"ieee":"B. Becker, V. Becker, H. Nerenz, and A. Schrader, “Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten,” <i>SOFW-Journal : Home &#38; personal care ingredients &#38; formulations; Deutsche Ausgabe</i>, vol. 140, no. 5, pp. 26–27, 30, 2014.","ufg":"<b>Becker, Barbara u. a.</b>: Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten, in: <i>SOFW-Journal : Home &#38; personal care ingredients &#38; formulations; Deutsche Ausgabe</i> 140 (2014), H. 5,  S. 26–27, 30.","short":"B. Becker, V. Becker, H. Nerenz, A. Schrader, SOFW-Journal : Home &#38; Personal Care Ingredients &#38; Formulations; Deutsche Ausgabe 140 (2014) 26–27, 30.","havard":"B. Becker, V. Becker, H. Nerenz, A. Schrader, Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten, SOFW-Journal : Home &#38; Personal Care Ingredients &#38; Formulations; Deutsche Ausgabe. 140 (2014) 26–27, 30.","chicago-de":"Becker, Barbara, Verena Becker, Heiko Nerenz und Andreas Schrader. 2014. Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. <i>SOFW-Journal : Home &#38; personal care ingredients &#38; formulations; Deutsche Ausgabe</i> 140, Nr. 5: 26–27, 30.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Becker, Barbara</span> ; <span style=\"font-variant:small-caps;\">Becker, Verena</span> ; <span style=\"font-variant:small-caps;\">Nerenz, Heiko</span> ; <span style=\"font-variant:small-caps;\">Schrader, Andreas</span>: Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. In: <i>SOFW-Journal : Home &#38; personal care ingredients &#38; formulations; Deutsche Ausgabe</i> Bd. 140, Verl. für Chemische Industrie, Ziolkowsky (2014), Nr. 5, S. 26–27, 30","apa":"Becker, B., Becker, V., Nerenz, H., &#38; Schrader, A. (2014). Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. <i>SOFW-Journal : Home &#38; Personal Care Ingredients &#38; Formulations; Deutsche Ausgabe</i>, <i>140</i>(5), 26–27, 30.","bjps":"<b>Becker B <i>et al.</i></b> (2014) Einsatz Der Durchflusszytometrie Zum Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten. <i>SOFW-Journal : Home &#38; personal care ingredients &#38; formulations; Deutsche Ausgabe</i> <b>140</b>, 26–27, 30.","chicago":"Becker, Barbara, Verena Becker, Heiko Nerenz, and Andreas Schrader. “Einsatz Der Durchflusszytometrie Zum Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten.” <i>SOFW-Journal : Home &#38; Personal Care Ingredients &#38; Formulations; Deutsche Ausgabe</i> 140, no. 5 (2014): 26–27, 30.","van":"Becker B, Becker V, Nerenz H, Schrader A. Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. SOFW-Journal : Home &#38; personal care ingredients &#38; formulations; Deutsche Ausgabe. 2014;140(5):26–7, 30.","ama":"Becker B, Becker V, Nerenz H, Schrader A. Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten. <i>SOFW-Journal : Home &#38; personal care ingredients &#38; formulations; Deutsche Ausgabe</i>. 2014;140(5):26-27, 30.","mla":"Becker, Barbara, et al. “Einsatz Der Durchflusszytometrie Zum Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten.” <i>SOFW-Journal : Home &#38; Personal Care Ingredients &#38; Formulations; Deutsche Ausgabe</i>, vol. 140, no. 5, 2014, pp. 26–27, 30."},"language":[{"iso":"eng"}]},{"abstract":[{"text":"Physiological consequences of adaptation to and continued feeding of a high-energetic diet were studied in eight non-pregnant, non-lactating dairy Holstein cows over a period of 16 weeks. The first six weeks served as an adaptation period from the low energetic straw-based diet (3.8 MJ NEL/kg DM) to the high-energetic ration (7.5 MJ NEL/kg DM). Intake of dry matter (DM) increased with dietary energy concentration from 9 to 20 kg/d up to week 9 to 12 and decreased thereafter. The initial live weight (LW) of 550 ± 60 kg was increased linearly and corresponded to an average daily LW gain of 2.3 ± 0.3 kg. Energy balance increased approximately nine-fold to a maximum of 114 MJ NEL/d in week 10. Ruminal fermentation pattern was completely changed from an acetate dominating profile to a propionate based one, which was paralleled by a marked increase in the rumen fluid endotoxin concentration. Unlike blood glucose concentration, which increased continuously, that of cholesterol and triglycerides started to increase after an initial stagnation. In conclusion, both ruminal adaptation to a high-energetic diet and the continued feeding of such a diet induced digestive and metabolic adaptations in non-pregnant, non-lactating cows characterised by a progressing positive energy balance.","lang":"eng"}],"year":"2014","department":[{"_id":"DEP4010"}],"place":"Abingdon","type":"scientific_journal_article","issue":"6","title":"Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity","author":[{"first_name":"Sven","full_name":"Dänicke, Sven","last_name":"Dänicke"},{"full_name":"Meyer, Ulrich","last_name":"Meyer","first_name":"Ulrich"},{"first_name":"Janine","last_name":"Winkler","full_name":"Winkler, Janine"},{"first_name":"Kirsten","last_name":"Schulz","full_name":"Schulz, Kirsten"},{"orcid":"0000-0002-4511-9537","last_name":"Ulrich","id":"85847","full_name":"Ulrich, Sebastian","first_name":"Sebastian"},{"last_name":"Frahm","full_name":"Frahm, Jana","first_name":"Jana"},{"last_name":"Kersten","full_name":"Kersten, Susanne","first_name":"Susanne"},{"full_name":"Rehage, Jürgen","last_name":"Rehage","first_name":"Jürgen"},{"first_name":"Gerhard","full_name":"Breves, Gerhard","last_name":"Breves"},{"first_name":"Susanne","full_name":"Häußler, Susanne","last_name":"Häußler"},{"first_name":"Helga","last_name":"Sauerwein","full_name":"Sauerwein, Helga"},{"first_name":"Lena","full_name":"Locher, Lena","last_name":"Locher"}],"quality_controlled":"1","publication_identifier":{"issn":["1745-039X","0003-942X"],"eissn":["1477-2817"]},"date_created":"2025-06-15T10:36:14Z","publication_status":"published","volume":68,"date_updated":"2025-06-18T12:23:01Z","extern":"1","status":"public","intvolume":"        68","doi":"10.1080/1745039x.2014.973243","publication":"Archives of Animal Nutrition","language":[{"iso":"eng"}],"citation":{"chicago":"Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian Ulrich, Jana Frahm, Susanne Kersten, et al. “Description of a Bovine Model for Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or Gravidity.” <i>Archives of Animal Nutrition</i> 68, no. 6 (2014): 460–77. <a href=\"https://doi.org/10.1080/1745039x.2014.973243\">https://doi.org/10.1080/1745039x.2014.973243</a>.","mla":"Dänicke, Sven, et al. “Description of a Bovine Model for Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or Gravidity.” <i>Archives of Animal Nutrition</i>, vol. 68, no. 6, 2014, pp. 460–77, <a href=\"https://doi.org/10.1080/1745039x.2014.973243\">https://doi.org/10.1080/1745039x.2014.973243</a>.","ieee":"S. Dänicke <i>et al.</i>, “Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity,” <i>Archives of Animal Nutrition</i>, vol. 68, no. 6, pp. 460–477, 2014, doi: <a href=\"https://doi.org/10.1080/1745039x.2014.973243\">10.1080/1745039x.2014.973243</a>.","chicago-de":"Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian Ulrich, Jana Frahm, Susanne Kersten, u. a. 2014. Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. <i>Archives of Animal Nutrition</i> 68, Nr. 6: 460–477. doi:<a href=\"https://doi.org/10.1080/1745039x.2014.973243\">10.1080/1745039x.2014.973243</a>, .","havard":"S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten, J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity, Archives of Animal Nutrition. 68 (2014) 460–477.","apa":"Dänicke, S., Meyer, U., Winkler, J., Schulz, K., Ulrich, S., Frahm, J., Kersten, S., Rehage, J., Breves, G., Häußler, S., Sauerwein, H., &#38; Locher, L. (2014). Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. <i>Archives of Animal Nutrition</i>, <i>68</i>(6), 460–477. <a href=\"https://doi.org/10.1080/1745039x.2014.973243\">https://doi.org/10.1080/1745039x.2014.973243</a>","bjps":"<b>Dänicke S <i>et al.</i></b> (2014) Description of a Bovine Model for Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or Gravidity. <i>Archives of Animal Nutrition</i> <b>68</b>, 460–477.","van":"Dänicke S, Meyer U, Winkler J, Schulz K, Ulrich S, Frahm J, et al. Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. Archives of Animal Nutrition. 2014;68(6):460–77.","ama":"Dänicke S, Meyer U, Winkler J, et al. Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. <i>Archives of Animal Nutrition</i>. 2014;68(6):460-477. doi:<a href=\"https://doi.org/10.1080/1745039x.2014.973243\">10.1080/1745039x.2014.973243</a>","ufg":"<b>Dänicke, Sven u. a.</b>: Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity, in: <i>Archives of Animal Nutrition</i> 68 (2014), H. 6,  S. 460–477.","short":"S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten, J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Archives of Animal Nutrition 68 (2014) 460–477.","din1505-2-1":"<span style=\"font-variant:small-caps;\"><span style=\"font-variant:small-caps;\">Dänicke, Sven</span> ; <span style=\"font-variant:small-caps;\">Meyer, Ulrich</span> ; <span style=\"font-variant:small-caps;\">Winkler, Janine</span> ; <span style=\"font-variant:small-caps;\">Schulz, Kirsten</span> ; <span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Frahm, Jana</span> ; <span style=\"font-variant:small-caps;\">Kersten, Susanne</span> ; <span style=\"font-variant:small-caps;\">Rehage, Jürgen</span> ; u. a.</span>: Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity. In: <i>Archives of Animal Nutrition</i> Bd. 68. Abingdon, Taylor &#38; Francis (2014), Nr. 6, S. 460–477"},"publisher":"Taylor & Francis","user_id":"83781","_id":"12979","keyword":["blood chemistry","dairy cows","endotoxins","energy balance","energy content","rumen fermentation"],"page":"460-477"},{"type":"scientific_journal_article","place":"Amsterdam","author":[{"id":"66516","full_name":"Dabisch-Ruthe, Mareike","last_name":"Dabisch-Ruthe","orcid":"https://orcid.org/0009-0008-7644-0826","first_name":"Mareike"},{"full_name":"Kuzaj, Patricia","last_name":"Kuzaj","first_name":"Patricia"},{"first_name":"Christian","full_name":"Götting, Christian","last_name":"Götting"},{"first_name":"Cornelius","full_name":"Knabbe, Cornelius","last_name":"Knabbe"},{"last_name":"Hendig","full_name":"Hendig, Doris","first_name":"Doris"}],"issue":"2","title":"Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum","abstract":[{"text":"Background\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder characterized by late onset and progressive calcification of elastic fibers in skin, eyes and the cardiovascular system, exemplifying a model for conditions characterized by soft tissue calcification.\r\nObjective\r\nThe aim of our study was to characterize cellular inorganic pyrophosphate (PPi) homeostasis in PXE.\r\nMethods\r\nGene expression of PPi metabolizing enzymes was determined by quantitative real-time PCR after incubation up to 21 days with or without addition of Na2HPO4. Extracellular and cytosolic PPi concentrations were measured by enzyme-linked bioluminescence assay. ALP and ENPP1 activity was determined spectrophotometrically. We further established a human cell culture model suitable for investigating PXE and related disorders without addition of artificial calcification triggers.\r\nResults\r\nIndependently of the experimental conditions, PXE fibroblasts revealed a higher degree of matrix calcification. We observed that matrix calcification was associated with altered gene expression of PPi metabolizing enzymes in PXE fibroblasts. In this context, PXE fibroblasts exhibited significantly higher expression of ALP and OPN and reduced mRNA expression and activity of ENPP1. Here, for the first time cytosolic and extracellular PPi levels were shown to be strongly reduced in PXE fibroblasts. We further showed that PPi concentration in bovine and human sera additives had a strong impact on matrix calcification. In a last experimental line, we demonstrated that addition of PPi analogs reduced matrix calcification of PXE fibroblasts most likely by reducing ALP and OPN mRNA expression, restoring ENPP1 activity and subsequently elevating PPi concentrations.\r\nConclusion\r\nThe results of our study along with recent findings point to the essential role of PPi as the central regulatory metabolites preventing matrix calcification in PXE. But what remains to be determined is the underlying molecular mechanism leading to depletion of PPi in PXE. We further suggest that supplementation of PPi analogs might counteract pathological calcification in PXE and related disorders.","lang":"eng"}],"year":"2014","department":[{"_id":"DEP4010"}],"publication_status":"published","volume":75,"date_updated":"2025-06-17T13:48:41Z","quality_controlled":"1","publication_identifier":{"eissn":["1873-569X"],"issn":["0923-1811"]},"date_created":"2025-06-17T06:52:59Z","status":"public","citation":{"din1505-2-1":"<span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Kuzaj, Patricia</span> ; <span style=\"font-variant:small-caps;\">Götting, Christian</span> ; <span style=\"font-variant:small-caps;\">Knabbe, Cornelius</span> ; <span style=\"font-variant:small-caps;\">Hendig, Doris</span>: Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. In: <i>Journal of Dermatological Science</i> Bd. 75. Amsterdam, Elsevier  (2014), Nr. 2, S. 109–120","ufg":"<b>Dabisch-Ruthe, Mareike u. a.</b>: Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum, in: <i>Journal of Dermatological Science</i> 75 (2014), H. 2,  S. 109–120.","short":"M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Journal of Dermatological Science 75 (2014) 109–120.","van":"Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal of Dermatological Science. 2014;75(2):109–20.","ama":"Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. <i>Journal of Dermatological Science</i>. 2014;75(2):109-120. doi:<a href=\"https://doi.org/10.1016/j.jdermsci.2014.04.015\">10.1016/j.jdermsci.2014.04.015</a>","bjps":"<b>Dabisch-Ruthe M <i>et al.</i></b> (2014) Pyrophosphates as a Major Inhibitor of Matrix Calcification in Pseudoxanthoma Elasticum. <i>Journal of Dermatological Science</i> <b>75</b>, 109–120.","chicago-de":"Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius Knabbe und Doris Hendig. 2014. Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. <i>Journal of Dermatological Science</i> 75, Nr. 2: 109–120. doi:<a href=\"https://doi.org/10.1016/j.jdermsci.2014.04.015\">10.1016/j.jdermsci.2014.04.015</a>, .","havard":"M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum, Journal of Dermatological Science. 75 (2014) 109–120.","apa":"Dabisch-Ruthe, M., Kuzaj, P., Götting, C., Knabbe, C., &#38; Hendig, D. (2014). Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. <i>Journal of Dermatological Science</i>, <i>75</i>(2), 109–120. <a href=\"https://doi.org/10.1016/j.jdermsci.2014.04.015\">https://doi.org/10.1016/j.jdermsci.2014.04.015</a>","ieee":"M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, and D. Hendig, “Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum,” <i>Journal of Dermatological Science</i>, vol. 75, no. 2, pp. 109–120, 2014, doi: <a href=\"https://doi.org/10.1016/j.jdermsci.2014.04.015\">10.1016/j.jdermsci.2014.04.015</a>.","chicago":"Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius Knabbe, and Doris Hendig. “Pyrophosphates as a Major Inhibitor of Matrix Calcification in Pseudoxanthoma Elasticum.” <i>Journal of Dermatological Science</i> 75, no. 2 (2014): 109–20. <a href=\"https://doi.org/10.1016/j.jdermsci.2014.04.015\">https://doi.org/10.1016/j.jdermsci.2014.04.015</a>.","mla":"Dabisch-Ruthe, Mareike, et al. “Pyrophosphates as a Major Inhibitor of Matrix Calcification in Pseudoxanthoma Elasticum.” <i>Journal of Dermatological Science</i>, vol. 75, no. 2, 2014, pp. 109–20, <a href=\"https://doi.org/10.1016/j.jdermsci.2014.04.015\">https://doi.org/10.1016/j.jdermsci.2014.04.015</a>."},"language":[{"iso":"eng"}],"publisher":"Elsevier ","_id":"12984","user_id":"83781","keyword":["Pseudoxanthoma elasticum","Calcification","Pyrophosphate","Bisphosphonate","ABCC6","Tissue nonspecific alkaline phosphate","Ectonucleotide pyrophosphatase 1"],"page":"109-120","publication":"Journal of Dermatological Science","doi":"10.1016/j.jdermsci.2014.04.015","intvolume":"        75"},{"status":"public","_id":"12985","user_id":"83781","keyword":["Pseudoxanthoma elasticum","Calcification","Tissue nonspecific alkaline phosphatase","Ectonucleotide pyrophosphatase 1","Ankylosis"],"page":"60-67","language":[{"iso":"eng"}],"citation":{"mla":"Dabisch-Ruthe, Mareike, et al. “Variants in Genes Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum.” <i>Clinical Biochemistry</i>, vol. 47, no. 15, 2014, pp. 60–67, <a href=\"https://doi.org/10.1016/j.clinbiochem.2014.07.003\">https://doi.org/10.1016/j.clinbiochem.2014.07.003</a>.","chicago":"Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel Issa, Christiane Szliska, Cornelius Knabbe, and Doris Hendig. “Variants in Genes Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum.” <i>Clinical Biochemistry</i> 47, no. 15 (2014): 60–67. <a href=\"https://doi.org/10.1016/j.clinbiochem.2014.07.003\">https://doi.org/10.1016/j.clinbiochem.2014.07.003</a>.","apa":"Dabisch-Ruthe, M., Brock, A., Kuzaj, P., Charbel Issa, P., Szliska, C., Knabbe, C., &#38; Hendig, D. (2014). Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. <i>Clinical Biochemistry</i>, <i>47</i>(15), 60–67. <a href=\"https://doi.org/10.1016/j.clinbiochem.2014.07.003\">https://doi.org/10.1016/j.clinbiochem.2014.07.003</a>","havard":"M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe, D. Hendig, Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum, Clinical Biochemistry. 47 (2014) 60–67.","chicago-de":"Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel Issa, Christiane Szliska, Cornelius Knabbe und Doris Hendig. 2014. Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. <i>Clinical Biochemistry</i> 47, Nr. 15: 60–67. doi:<a href=\"https://doi.org/10.1016/j.clinbiochem.2014.07.003\">10.1016/j.clinbiochem.2014.07.003</a>, .","ieee":"M. Dabisch-Ruthe <i>et al.</i>, “Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum,” <i>Clinical Biochemistry</i>, vol. 47, no. 15, pp. 60–67, 2014, doi: <a href=\"https://doi.org/10.1016/j.clinbiochem.2014.07.003\">10.1016/j.clinbiochem.2014.07.003</a>.","ama":"Dabisch-Ruthe M, Brock A, Kuzaj P, et al. Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. <i>Clinical Biochemistry</i>. 2014;47(15):60-67. doi:<a href=\"https://doi.org/10.1016/j.clinbiochem.2014.07.003\">10.1016/j.clinbiochem.2014.07.003</a>","van":"Dabisch-Ruthe M, Brock A, Kuzaj P, Charbel Issa P, Szliska C, Knabbe C, et al. Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. Clinical Biochemistry. 2014;47(15):60–7.","bjps":"<b>Dabisch-Ruthe M <i>et al.</i></b> (2014) Variants in Genes Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum. <i>Clinical Biochemistry</i> <b>47</b>, 60–67.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Brock, Alexander</span> ; <span style=\"font-variant:small-caps;\">Kuzaj, Patricia</span> ; <span style=\"font-variant:small-caps;\">Charbel Issa, Peter</span> ; <span style=\"font-variant:small-caps;\">Szliska, Christiane</span> ; <span style=\"font-variant:small-caps;\">Knabbe, Cornelius</span> ; <span style=\"font-variant:small-caps;\">Hendig, Doris</span>: Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum. In: <i>Clinical Biochemistry</i> Bd. 47. Amsterdam, Elsevier (2014), Nr. 15, S. 60–67","short":"M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe, D. Hendig, Clinical Biochemistry 47 (2014) 60–67.","ufg":"<b>Dabisch-Ruthe, Mareike u. a.</b>: Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum, in: <i>Clinical Biochemistry</i> 47 (2014), H. 15,  S. 60–67."},"publisher":"Elsevier","intvolume":"        47","doi":"10.1016/j.clinbiochem.2014.07.003","publication":"Clinical Biochemistry","title":"Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum","issue":"15","author":[{"first_name":"Mareike","full_name":"Dabisch-Ruthe, Mareike","id":"66516","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe"},{"first_name":"Alexander","full_name":"Brock, Alexander","last_name":"Brock"},{"first_name":"Patricia","last_name":"Kuzaj","full_name":"Kuzaj, Patricia"},{"first_name":"Peter","full_name":"Charbel Issa, Peter","last_name":"Charbel Issa"},{"full_name":"Szliska, Christiane","last_name":"Szliska","first_name":"Christiane"},{"full_name":"Knabbe, Cornelius","last_name":"Knabbe","first_name":"Cornelius"},{"first_name":"Doris","full_name":"Hendig, Doris","last_name":"Hendig"}],"place":"Amsterdam","type":"scientific_journal_article","department":[{"_id":"DEP4010"}],"abstract":[{"lang":"eng","text":"Objectives\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder characterized by progressive calcification and fragmentation of elastic fibers. Because of the great clinical variability between PXE patients the involvement of modifier genes was recently suggested. Therefore, we investigated the association of single nucleotide variants (SNVs) in selected candidate genes known to regulate cellular pyrophosphate metabolism.\r\nDesign and methods\r\nWe used RLFP analyses to evaluate the distribution of SNVs in alkaline phosphatase (ALP), ectonucleotide pyrophosphatase 1 (ENPP1) and ankylosis (ANKH) in DNA samples from 190 German PXE patients and 190 age- and sex-matched healthy controls. Statistical analyses were performed using Fisher exact test and Bonferroni correction.\r\nResults\r\nThe screening revealed three different SNVs in three genes, which were associated with PXE. The SNV c.1190-65C > A (rs1780329, minor allele frequency (MAF) patients: 0.17; controls: 0.11; P = 0.04) in the ALP gene was significantly more frequent in PXE patients. Furthermore, PXE was highly associated with ANKH p.A98A genotype TT (P = 0.0012), although the MAF was not different between patients and controls. After correction for multiple testing according to the Bonferroni method, one SNV in the ENPP1 gene (c.313 + 9G > T, rs7773477) remained significantly associated with PXE with significantly higher MAF values in the patient cohort (MAF: 0.04 vs. 0.00; P = 0.0024) and a high association with PXE susceptibility (OR 27.96).\r\nConclusion\r\nPolymorphisms in ALP, ENPP1 and ANKH are important genetic risk factors contributing to PXE."}],"year":"2014","volume":47,"publication_status":"published","date_updated":"2025-06-17T13:48:52Z","publication_identifier":{"issn":["0009-9120"],"eissn":["1873-2933"]},"date_created":"2025-06-17T06:53:30Z"},{"year":"2014","abstract":[{"text":"Background\r\nDysregulations in cholesterol and lipid metabolism have been linked to human diseases like hypercholesterolemia, atherosclerosis or the metabolic syndrome. Many ABC transporters are involved in trafficking of metabolites derived from these pathways. Pseudoxanthoma elasticum (PXE), an autosomal-recessive disease caused by ABCC6 mutations, is characterized by atherogenesis and soft tissue calcification.\r\nMethods\r\nIn this study we investigated the regulation of cholesterol biosynthesis in human dermal fibroblasts from PXE patients and healthy controls.\r\nResults\r\nGene expression analysis of 84 targets indicated dysregulations in cholesterol metabolism in PXE fibroblasts. Transcript levels of ABCC6 were strongly increased in lipoprotein-deficient serum (LPDS) and under serum starvation in healthy controls. For the first time, increased HMG CoA reductase activities were found in PXE fibroblasts. We further observed strongly elevated transcript and protein levels for the proprotein convertase subtilisin/kexin type 9 (PCSK9), as well as a significant reduction in APOE mRNA expression in PXE.\r\nConclusion\r\nIncreased cholesterol biosynthesis, elevated PCSK9 levels and reduced APOE mRNA expression newly found in PXE fibroblasts could enforce atherogenesis and cardiovascular risk in PXE patients. Moreover, the increase in ABCC6 expression accompanied by the induction of cholesterol biosynthesis supposes a functional role for ABCC6 in human lipoprotein and cholesterol homeostasis.","lang":"eng"}],"department":[{"_id":"DEP4010"}],"type":"scientific_journal_article","place":"London","author":[{"last_name":"Kuzaj","full_name":"Kuzaj, Patricia","first_name":"Patricia"},{"last_name":"Kuhn","full_name":"Kuhn, Joachim","first_name":"Joachim"},{"first_name":"Mareike","id":"66516","full_name":"Dabisch-Ruthe, Mareike","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe"},{"first_name":"Isabel","last_name":"Faust","full_name":"Faust, Isabel"},{"first_name":"Christian","full_name":"Götting, Christian","last_name":"Götting"},{"first_name":"Cornelius","full_name":"Knabbe, Cornelius","last_name":"Knabbe"},{"last_name":"Hendig","full_name":"Hendig, Doris","first_name":"Doris"}],"title":"ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?","issue":"1","quality_controlled":"1","date_created":"2025-06-17T06:53:58Z","publication_identifier":{"eissn":["1476-511X"]},"date_updated":"2025-06-17T13:49:15Z","volume":13,"publication_status":"published","status":"public","article_number":"118","publication":"Lipids in Health and Disease","doi":"10.1186/1476-511x-13-118","intvolume":"        13","publisher":"Biomed Central ","citation":{"van":"Kuzaj P, Kuhn J, Dabisch-Ruthe M, Faust I, Götting C, Knabbe C, et al. ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? Lipids in Health and Disease. 2014;13(1).","ama":"Kuzaj P, Kuhn J, Dabisch-Ruthe M, et al. ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? <i>Lipids in Health and Disease</i>. 2014;13(1). doi:<a href=\"https://doi.org/10.1186/1476-511x-13-118\">10.1186/1476-511x-13-118</a>","mla":"Kuzaj, Patricia, et al. “ABCC6- a New Player in Cellular Cholesterol and Lipoprotein Metabolism?” <i>Lipids in Health and Disease</i>, vol. 13, no. 1, 118, 2014, <a href=\"https://doi.org/10.1186/1476-511x-13-118\">https://doi.org/10.1186/1476-511x-13-118</a>.","chicago":"Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust, Christian Götting, Cornelius Knabbe, and Doris Hendig. “ABCC6- a New Player in Cellular Cholesterol and Lipoprotein Metabolism?” <i>Lipids in Health and Disease</i> 13, no. 1 (2014). <a href=\"https://doi.org/10.1186/1476-511x-13-118\">https://doi.org/10.1186/1476-511x-13-118</a>.","bjps":"<b>Kuzaj P <i>et al.</i></b> (2014) ABCC6- a New Player in Cellular Cholesterol and Lipoprotein Metabolism? <i>Lipids in Health and Disease</i> <b>13</b>.","apa":"Kuzaj, P., Kuhn, J., Dabisch-Ruthe, M., Faust, I., Götting, C., Knabbe, C., &#38; Hendig, D. (2014). ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? <i>Lipids in Health and Disease</i>, <i>13</i>(1), Article 118. <a href=\"https://doi.org/10.1186/1476-511x-13-118\">https://doi.org/10.1186/1476-511x-13-118</a>","havard":"P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D. Hendig, ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?, Lipids in Health and Disease. 13 (2014).","chicago-de":"Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust, Christian Götting, Cornelius Knabbe und Doris Hendig. 2014. ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? <i>Lipids in Health and Disease</i> 13, Nr. 1. doi:<a href=\"https://doi.org/10.1186/1476-511x-13-118\">10.1186/1476-511x-13-118</a>, .","din1505-2-1":"<span style=\"font-variant:small-caps;\">Kuzaj, Patricia</span> ; <span style=\"font-variant:small-caps;\">Kuhn, Joachim</span> ; <span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Faust, Isabel</span> ; <span style=\"font-variant:small-caps;\">Götting, Christian</span> ; <span style=\"font-variant:small-caps;\">Knabbe, Cornelius</span> ; <span style=\"font-variant:small-caps;\">Hendig, Doris</span>: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? In: <i>Lipids in Health and Disease</i> Bd. 13. London, Biomed Central  (2014), Nr. 1","short":"P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D. Hendig, Lipids in Health and Disease 13 (2014).","ieee":"P. Kuzaj <i>et al.</i>, “ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?,” <i>Lipids in Health and Disease</i>, vol. 13, no. 1, Art. no. 118, 2014, doi: <a href=\"https://doi.org/10.1186/1476-511x-13-118\">10.1186/1476-511x-13-118</a>.","ufg":"<b>Kuzaj, Patricia u. a.</b>: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?, in: <i>Lipids in Health and Disease</i> 13 (2014), H. 1."},"language":[{"iso":"eng"}],"_id":"12986","user_id":"83781","keyword":["Pseudoxanthoma elasticum","ABC transporter","ABCC6","Cholesterol biosynthesis","Atherosclerosis","HMG CoA reductase","SREBP2","PCSK9","LDLR","APOE"]},{"intvolume":"         9","publication":"PLOS ONE / Public Library of Science ","doi":"10.1371/journal.pone.0108336","publisher":"Public Library of Science (PLoS)","language":[{"iso":"eng"}],"citation":{"ieee":"P. Kuzaj <i>et al.</i>, “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls,” <i>PLOS ONE / Public Library of Science </i>, vol. 9, no. 9, Art. no. e108336, 2014, doi: <a href=\"https://doi.org/10.1371/journal.pone.0108336\">10.1371/journal.pone.0108336</a>.","havard":"P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe, C. Knabbe, D. Hendig, Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls, PLOS ONE / Public Library of Science . 9 (2014).","chicago-de":"Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe und Doris Hendig. 2014. Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. <i>PLOS ONE / Public Library of Science </i> 9, Nr. 9. doi:<a href=\"https://doi.org/10.1371/journal.pone.0108336\">10.1371/journal.pone.0108336</a>, .","apa":"Kuzaj, P., Kuhn, J., Michalek, R. D., Karoly, E. D., Faust, I., Dabisch-Ruthe, M., Knabbe, C., &#38; Hendig, D. (2014). Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. <i>PLOS ONE / Public Library of Science </i>, <i>9</i>(9), Article e108336. <a href=\"https://doi.org/10.1371/journal.pone.0108336\">https://doi.org/10.1371/journal.pone.0108336</a>","chicago":"Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe, and Doris Hendig. “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls.” <i>PLOS ONE / Public Library of Science </i> 9, no. 9 (2014). <a href=\"https://doi.org/10.1371/journal.pone.0108336\">https://doi.org/10.1371/journal.pone.0108336</a>.","mla":"Kuzaj, Patricia, et al. “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls.” <i>PLOS ONE / Public Library of Science </i>, vol. 9, no. 9, e108336, 2014, <a href=\"https://doi.org/10.1371/journal.pone.0108336\">https://doi.org/10.1371/journal.pone.0108336</a>.","ufg":"<b>Kuzaj, Patricia u. a.</b>: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls, in: <i>PLOS ONE / Public Library of Science </i> 9 (2014), H. 9.","short":"P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe, C. Knabbe, D. Hendig, PLOS ONE / Public Library of Science  9 (2014).","din1505-2-1":"<span style=\"font-variant:small-caps;\">Kuzaj, Patricia</span> ; <span style=\"font-variant:small-caps;\">Kuhn, Joachim</span> ; <span style=\"font-variant:small-caps;\">Michalek, Ryan D.</span> ; <span style=\"font-variant:small-caps;\">Karoly, Edward D.</span> ; <span style=\"font-variant:small-caps;\">Faust, Isabel</span> ; <span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Knabbe, Cornelius</span> ; <span style=\"font-variant:small-caps;\">Hendig, Doris</span>: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. In: <i>PLOS ONE / Public Library of Science </i> Bd. 9. San Francisco, California, US, Public Library of Science (PLoS) (2014), Nr. 9","bjps":"<b>Kuzaj P <i>et al.</i></b> (2014) Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. <i>PLOS ONE / Public Library of Science </i> <b>9</b>.","ama":"Kuzaj P, Kuhn J, Michalek RD, et al. Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. <i>PLOS ONE / Public Library of Science </i>. 2014;9(9). doi:<a href=\"https://doi.org/10.1371/journal.pone.0108336\">10.1371/journal.pone.0108336</a>","van":"Kuzaj P, Kuhn J, Michalek RD, Karoly ED, Faust I, Dabisch-Ruthe M, et al. Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls. PLOS ONE / Public Library of Science . 2014;9(9)."},"_id":"12987","user_id":"83781","status":"public","article_number":"e108336","quality_controlled":"1","date_created":"2025-06-17T06:54:30Z","publication_identifier":{"eissn":["1932-6203"]},"date_updated":"2025-06-17T13:49:26Z","volume":9,"publication_status":"published","year":"2014","abstract":[{"text":"Mutations in the ABC transporter ABCC6 were recently identified as cause of Pseudoxanthoma elasticum (PXE), a rare genetic disorder characterized by progressive mineralization of elastic fibers. We used an untargeted metabolic approach to identify biochemical differences between human dermal fibroblasts from healthy controls and PXE patients in an attempt to find a link between ABCC6 deficiency, cellular metabolic alterations and disease pathogenesis. 358 compounds were identified by mass spectrometry covering lipids, amino acids, peptides, carbohydrates, nucleotides, vitamins and cofactors, xenobiotics and energy metabolites. We found substantial differences in glycerophospholipid composition, leucine dipeptides, and polypeptides as well as alterations in pantothenate and guanine metabolism to be significantly associated with PXE pathogenesis. These findings can be linked to extracellular matrix remodeling and increased oxidative stress, which reflect characteristic hallmarks of PXE. Our study could facilitate a better understanding of biochemical pathways involved in soft tissue mineralization.","lang":"eng"}],"department":[{"_id":"DEP4010"}],"place":"San Francisco, California, US","type":"scientific_journal_article","title":"Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls","issue":"9","author":[{"first_name":"Patricia","full_name":"Kuzaj, Patricia","last_name":"Kuzaj"},{"last_name":"Kuhn","full_name":"Kuhn, Joachim","first_name":"Joachim"},{"last_name":"Michalek","full_name":"Michalek, Ryan D.","first_name":"Ryan D."},{"full_name":"Karoly, Edward D.","last_name":"Karoly","first_name":"Edward D."},{"first_name":"Isabel","full_name":"Faust, Isabel","last_name":"Faust"},{"first_name":"Mareike","last_name":"Dabisch-Ruthe","orcid":"https://orcid.org/0009-0008-7644-0826","id":"66516","full_name":"Dabisch-Ruthe, Mareike"},{"first_name":"Cornelius","last_name":"Knabbe","full_name":"Knabbe, Cornelius"},{"first_name":"Doris","last_name":"Hendig","full_name":"Hendig, Doris"}]},{"date_created":"2021-03-30T07:24:31Z","date_updated":"2024-05-24T06:46:24Z","_id":"5348","user_id":"83779","language":[{"iso":"ger"}],"citation":{"short":"K. Schwarzer, J. Schneider, B. Becker, U. Müller, in: 2012.","ufg":"<b>Schwarzer, Knut u. a.</b>: Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food, in: o. Hg.: o. O. 2012.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Schwarzer, Knut</span> ; <span style=\"font-variant:small-caps;\">Schneider, Jan</span> ; <span style=\"font-variant:small-caps;\">Becker, Barbara</span> ; <span style=\"font-variant:small-caps;\">Müller, Ulrich</span>: Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. In: , 2012","bjps":"<b>Schwarzer K <i>et al.</i></b> (2012) Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food.","ama":"Schwarzer K, Schneider J, Becker B, Müller U. Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. In: ; 2012.","van":"Schwarzer K, Schneider J, Becker B, Müller U. Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. In 2012.","ieee":"K. Schwarzer, J. Schneider, B. Becker, and U. Müller, “Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food,” presented at the ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik , Stuttgart - Hohenheim, 2012.","apa":"Schwarzer, K., Schneider, J., Becker, B., &#38; Müller, U. (2012). <i>Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food</i>. ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik , Stuttgart - Hohenheim.","havard":"K. Schwarzer, J. Schneider, B. Becker, U. Müller, Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food, in: 2012.","chicago-de":"Schwarzer, Knut, Jan Schneider, Barbara Becker und Ulrich Müller. 2012. Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food. In: .","mla":"Schwarzer, Knut, et al. <i>Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food</i>. 2012.","chicago":"Schwarzer, Knut, Jan Schneider, Barbara Becker, and Ulrich Müller. “Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food,” 2012."},"department":[{"_id":"DEP4009"},{"_id":"DEP4023"},{"_id":"DEP4010"},{"_id":"DEP4018"}],"year":"2012","title":"Entkeimungsauslegung mit der \"Lemgo D- and z-value Database for Food","status":"public","author":[{"first_name":"Knut","last_name":"Schwarzer","id":"13329","full_name":"Schwarzer, Knut"},{"first_name":"Jan","full_name":"Schneider, Jan","id":"13209","orcid":"0000-0001-6401-8873","last_name":"Schneider"},{"first_name":"Barbara","last_name":"Becker","full_name":"Becker, Barbara","id":"12640"},{"first_name":"Ulrich","full_name":"Müller, Ulrich","id":"12119","last_name":"Müller"}],"conference":{"end_date":"2012-03-21","start_date":"2012-03-19","location":"Stuttgart - Hohenheim","name":"ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik "},"type":"conference"},{"intvolume":"        12","doi":"10.1186/1471-2334-12-163","publication":"BMC Infectious Diseases","_id":"12983","keyword":["Respiratory Virus","Elution Volume","Multiplex Assay","Multiple Infection","Bocavirus"],"user_id":"83781","publisher":"Springer","language":[{"iso":"eng"}],"citation":{"apa":"Dabisch-Ruthe, M., Vollmer, T., Adams, O., Knabbe, C., &#38; Dreier, J. (2012). Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. <i>BMC Infectious Diseases</i>, <i>12</i>(1), Article 163. <a href=\"https://doi.org/10.1186/1471-2334-12-163\">https://doi.org/10.1186/1471-2334-12-163</a>","chicago-de":"Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe und Jens Dreier. 2012. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. <i>BMC Infectious Diseases</i> 12, Nr. 1. doi:<a href=\"https://doi.org/10.1186/1471-2334-12-163\">10.1186/1471-2334-12-163</a>, .","havard":"M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, BMC Infectious Diseases. 12 (2012).","ieee":"M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, and J. Dreier, “Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay,” <i>BMC Infectious Diseases</i>, vol. 12, no. 1, Art. no. 163, 2012, doi: <a href=\"https://doi.org/10.1186/1471-2334-12-163\">10.1186/1471-2334-12-163</a>.","mla":"Dabisch-Ruthe, Mareike, et al. “Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” <i>BMC Infectious Diseases</i>, vol. 12, no. 1, 163, 2012, <a href=\"https://doi.org/10.1186/1471-2334-12-163\">https://doi.org/10.1186/1471-2334-12-163</a>.","chicago":"Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe, and Jens Dreier. “Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” <i>BMC Infectious Diseases</i> 12, no. 1 (2012). <a href=\"https://doi.org/10.1186/1471-2334-12-163\">https://doi.org/10.1186/1471-2334-12-163</a>.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Vollmer, Tanja</span> ; <span style=\"font-variant:small-caps;\">Adams, Ortwin</span> ; <span style=\"font-variant:small-caps;\">Knabbe, Cornelius</span> ; <span style=\"font-variant:small-caps;\">Dreier, Jens</span>: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. In: <i>BMC Infectious Diseases</i> Bd. 12. Berlin ; Heidelberg, Springer (2012), Nr. 1","short":"M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, BMC Infectious Diseases 12 (2012).","ufg":"<b>Dabisch-Ruthe, Mareike u. a.</b>: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, in: <i>BMC Infectious Diseases</i> 12 (2012), H. 1.","van":"Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infectious Diseases. 2012;12(1).","ama":"Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. <i>BMC Infectious Diseases</i>. 2012;12(1). doi:<a href=\"https://doi.org/10.1186/1471-2334-12-163\">10.1186/1471-2334-12-163</a>","bjps":"<b>Dabisch-Ruthe M <i>et al.</i></b> (2012) Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay. <i>BMC Infectious Diseases</i> <b>12</b>."},"status":"public","article_number":"163","date_created":"2025-06-17T06:52:13Z","publication_identifier":{"eissn":["1471-2334"]},"quality_controlled":"1","date_updated":"2025-06-17T13:49:03Z","volume":12,"publication_status":"published","department":[{"_id":"DEP4010"}],"year":"2012","abstract":[{"lang":"eng","text":"Background\r\nA broad spectrum of pathogens is causative for respiratory tract infections, but symptoms are mostly similar. Therefore, the identification of the causative viruses and bacteria is only feasible using multiplex PCR or several monoplex PCR tests in parallel.\r\nMethods\r\nThe analytical sensitivity of three multiplex PCR assays, RespiFinder-19, RespiFinder-SMART-22 and xTAG-Respiratory-Virus-Panel-Fast-Assay (RVP), were compared to monoplex real-time PCR with quantified standardized control material. All assays include the most common respiratory pathogens.\r\nResults\r\nTo compare the analytical sensitivity of the multiplex assays, samples were inoculated with 13 different quantified viruses in the range of 101 to 105 copies/ml. Concordant results were received for rhinovirus, whereas the RVP detected influenzavirus, RSV and hMPV more frequently in low concentrations. The RespiFinder-19 and the RespiFinder-SMART-22 showed a higher analytical sensitivity for adenoviruses and coronaviruses, whereas the RVP was incapable to detect adenovirus and coronavirus in concentrations of 104 copies/ml. The RespiFinder-19 and RespiFinder-SMART-22A did not detect influenzaviruses (104 copies/ml) and RSV (103 copies/ml). The detection of all 13 viruses in one sample was only achieved using monoplex PCR. To analyze possible competitive amplification reactions between the different viruses, samples were further inoculated with only 4 different viruses in one sample. Compared to the detection of 13 viruses in parallel, only a few differences were found.\r\nThe incidence of respiratory viruses was compared in tracheal secretion (TS) samples (n = 100) of mechanically ventilated patients in winter (n = 50) and summer (n = 50). In winter, respiratory viruses were detected in 32 TS samples (64%) by RespiFinder-19, whereas the detection rate with RVP was only 22%. The most frequent viruses were adenovirus (32%) and PIV-2 (20%). Multiple infections were detected in 16 TS samples (32%) by RespiFinder-19. Fewer infections were found in summer (RespiFinder-19: 20%; RVP: 6%). All positive results were verified using monoplex PCR.\r\nConclusions\r\nMultiplex PCR tests have a broad spectrum of pathogens to test at a time. Analysis of multiple inoculated samples revealed a different focus of the detected virus types by the three assays. Analysis of clinical samples showed a high concordance of detected viruses by the RespiFinder-19 compared to monoplex tests."}],"issue":"1","title":"Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay","author":[{"first_name":"Mareike","last_name":"Dabisch-Ruthe","orcid":"https://orcid.org/0009-0008-7644-0826","id":"66516","full_name":"Dabisch-Ruthe, Mareike"},{"last_name":"Vollmer","full_name":"Vollmer, Tanja","first_name":"Tanja"},{"full_name":"Adams, Ortwin","last_name":"Adams","first_name":"Ortwin"},{"last_name":"Knabbe","full_name":"Knabbe, Cornelius","first_name":"Cornelius"},{"last_name":"Dreier","full_name":"Dreier, Jens","first_name":"Jens"}],"place":"Berlin ; Heidelberg","type":"scientific_journal_article"},{"type":"journal_article","title":"Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim","issue":"5","status":"public","author":[{"first_name":"Jan","id":"13209","full_name":"Schneider, Jan","orcid":"0000-0001-6401-8873","last_name":"Schneider"},{"last_name":"Müller","id":"12119","full_name":"Müller, Ulrich","first_name":"Ulrich"},{"last_name":"Schwarzer","id":"13329","full_name":"Schwarzer, Knut","first_name":"Knut"},{"id":"12640","full_name":"Becker, Barbara","last_name":"Becker","first_name":"Barbara"},{"last_name":"Wilhelm","id":"12013","full_name":"Wilhelm, Patrick","first_name":"Patrick"}],"year":2010,"department":[{"_id":"DEP4010"},{"_id":"DEP4009"},{"_id":"DEP4018"}],"publisher":"Fachverlag Hans Carl GmbH","language":[{"iso":"eng"}],"citation":{"ufg":"<b>Schneider, Jan u. a.</b>: Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim, in: <i>Brauwelt International</i> (2010), H. 5,  S. 252–256.","short":"J. Schneider, U. Müller, K. Schwarzer, B. Becker, P. Wilhelm, Brauwelt International (2010) 252–256.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Schneider, Jan</span> ; <span style=\"font-variant:small-caps;\">Müller, Ulrich</span> ; <span style=\"font-variant:small-caps;\">Schwarzer, Knut</span> ; <span style=\"font-variant:small-caps;\">Becker, Barbara</span> ; <span style=\"font-variant:small-caps;\">Wilhelm, Patrick</span>: Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. In: <i>Brauwelt International</i>, Fachverlag Hans Carl GmbH (2010), Nr. 5, S. 252–256","bjps":"<b>Schneider J <i>et al.</i></b> (2010) Lemgo Database for D- and z-Values: Useful for Beverage Spoilage Microorgansim. <i>Brauwelt International</i> 252–256.","van":"Schneider J, Müller U, Schwarzer K, Becker B, Wilhelm P. Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International. 2010;(5):252–6.","ama":"Schneider J, Müller U, Schwarzer K, Becker B, Wilhelm P. Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. <i>Brauwelt International</i>. 2010;(5):252-256.","ieee":"J. Schneider, U. Müller, K. Schwarzer, B. Becker, and P. Wilhelm, “Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim,” <i>Brauwelt International</i>, no. 5, pp. 252–256, 2010.","chicago-de":"Schneider, Jan, Ulrich Müller, Knut Schwarzer, Barbara Becker und Patrick Wilhelm. 2010. Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. <i>Brauwelt International</i>, Nr. 5: 252–256.","havard":"J. Schneider, U. Müller, K. Schwarzer, B. Becker, P. Wilhelm, Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim, Brauwelt International. (2010) 252–256.","apa":"Schneider, J., Müller, U., Schwarzer, K., Becker, B., &#38; Wilhelm, P. (2010). Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim. <i>Brauwelt International</i>, <i>5</i>, 252–256.","chicago":"Schneider, Jan, Ulrich Müller, Knut Schwarzer, Barbara Becker, and Patrick Wilhelm. “Lemgo Database for D- and z-Values: Useful for Beverage Spoilage Microorgansim.” <i>Brauwelt International</i>, no. 5 (2010): 252–56.","mla":"Schneider, Jan, et al. “Lemgo Database for D- and z-Values: Useful for Beverage Spoilage Microorgansim.” <i>Brauwelt International</i>, no. 5, 2010, pp. 252–56."},"date_updated":"2023-03-15T13:49:42Z","page":"252 - 256","publication_status":"published","_id":"2748","user_id":"12013","date_created":"2020-06-02T10:24:20Z","publication_identifier":{"issn":["0934-9340"]},"publication":"Brauwelt International"},{"type":"journal_article","issue":"18","title":"Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen","status":"public","author":[{"last_name":"Schwarzer","full_name":"Schwarzer, Knut","id":"13329","first_name":"Knut"},{"id":"13209","full_name":"Schneider, Jan","orcid":"0000-0001-6401-8873","last_name":"Schneider","first_name":"Jan"},{"last_name":"Müller","id":"12119","full_name":"Müller, Ulrich","first_name":"Ulrich"},{"last_name":"Becker","id":"12640","full_name":"Becker, Barbara","first_name":"Barbara"},{"first_name":"Patrick","last_name":"Wilhelm","id":"12013","full_name":"Wilhelm, Patrick"}],"year":2010,"department":[{"_id":"DEP4010"},{"_id":"DEP4009"},{"_id":"DEP4018"}],"publisher":"Fachverlag Hans Carl GmbH","language":[{"iso":"eng"}],"citation":{"ieee":"K. Schwarzer, J. Schneider, U. Müller, B. Becker, and P. Wilhelm, “Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen,” <i>Brauwelt</i>, vol. 150, no. 18, pp. 540–544, 2010.","apa":"Schwarzer, K., Schneider, J., Müller, U., Becker, B., &#38; Wilhelm, P. (2010). Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. <i>Brauwelt</i>, <i>150</i>(18), 540–544.","chicago-de":"Schwarzer, Knut, Jan Schneider, Ulrich Müller, Barbara Becker und Patrick Wilhelm. 2010. Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. <i>Brauwelt</i> 150, Nr. 18: 540–544.","havard":"K. Schwarzer, J. Schneider, U. Müller, B. Becker, P. Wilhelm, Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen, Brauwelt. 150 (2010) 540–544.","mla":"Schwarzer, Knut, et al. “Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für Getränkeschädliche Mikroorganismen.” <i>Brauwelt</i>, vol. 150, no. 18, 2010, pp. 540–44.","chicago":"Schwarzer, Knut, Jan Schneider, Ulrich Müller, Barbara Becker, and Patrick Wilhelm. “Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für Getränkeschädliche Mikroorganismen.” <i>Brauwelt</i> 150, no. 18 (2010): 540–44.","short":"K. Schwarzer, J. Schneider, U. Müller, B. Becker, P. Wilhelm, Brauwelt 150 (2010) 540–544.","ufg":"<b>Schwarzer, Knut u. a.</b>: Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen, in: <i>Brauwelt</i> 150 (2010), H. 18,  S. 540–544.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Schwarzer, Knut</span> ; <span style=\"font-variant:small-caps;\">Schneider, Jan</span> ; <span style=\"font-variant:small-caps;\">Müller, Ulrich</span> ; <span style=\"font-variant:small-caps;\">Becker, Barbara</span> ; <span style=\"font-variant:small-caps;\">Wilhelm, Patrick</span>: Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. In: <i>Brauwelt</i> Bd. 150, Fachverlag Hans Carl GmbH (2010), Nr. 18, S. 540–544","bjps":"<b>Schwarzer K <i>et al.</i></b> (2010) Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für Getränkeschädliche Mikroorganismen. <i>Brauwelt</i> <b>150</b>, 540–544.","van":"Schwarzer K, Schneider J, Müller U, Becker B, Wilhelm P. Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt. 2010;150(18):540–4.","ama":"Schwarzer K, Schneider J, Müller U, Becker B, Wilhelm P. Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. <i>Brauwelt</i>. 2010;150(18):540-544."},"date_updated":"2023-03-15T13:49:42Z","page":"540 - 544","_id":"2763","publication_status":"published","user_id":"12013","volume":150,"date_created":"2020-06-03T07:13:41Z","publication_identifier":{"issn":["\t0724-696X"]},"intvolume":"       150","publication":"Brauwelt"},{"date_created":"2020-05-18T09:40:45Z","publication":"Fleischwirtschaft ","page":"61-65","date_updated":"2023-03-15T13:49:39Z","_id":"2361","user_id":"74222","publication_date":"19.03.2019","language":[{"iso":"eng"}],"citation":{"din1505-2-1":"<span style=\"font-variant:small-caps;\">Becker, Barbara</span> ; <span style=\"font-variant:small-caps;\">Hildebrandt, Goetz</span> ; <span style=\"font-variant:small-caps;\">Erol, Irfan </span> ; <span style=\"font-variant:small-caps;\">Kleer, Josef</span> ; <span style=\"font-variant:small-caps;\">Manopas, Aranya Sira</span>: Von der Döner - Prüfung zum Döner - Heariing. In: <i>Fleischwirtschaft </i> (2009), Nr. 7","ufg":"<b>Becker, Barbara et. al. (2009)</b>: Von der Döner - Prüfung zum Döner - Heariing, in: <i>Fleischwirtschaft </i> (<i>7</i>) (<i>2009</i>), S. 61–65.","short":"B. Becker, G. Hildebrandt, I. Erol, J. Kleer, A.S. Manopas, Fleischwirtschaft  (2009) 61–65.","ama":"Becker B, Hildebrandt G, Erol I, Kleer J, Manopas AS. Von der Döner - Prüfung zum Döner - Heariing. <i>Fleischwirtschaft </i>. 2009:61-65.","van":"Becker B, Hildebrandt G, Erol I, Kleer J, Manopas AS. Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft . 2009;61–5.","bjps":"<b>Becker B <i>et al.</i></b> (2009) Von Der Döner - Prüfung Zum Döner - Heariing. <i>Fleischwirtschaft </i>, 61–65.","havard":"B. Becker, G. Hildebrandt, I. Erol, J. Kleer, A.S. Manopas, Von der Döner - Prüfung zum Döner - Heariing, Fleischwirtschaft . (2009) 61–65.","chicago-de":"Becker, Barbara, Goetz Hildebrandt, Irfan  Erol, Josef Kleer und Aranya Sira Manopas. 2009. Von der Döner - Prüfung zum Döner - Heariing. <i>Fleischwirtschaft </i>.","apa":"Becker, B., Hildebrandt, G., Erol, I., Kleer, J., &#38; Manopas, A. S. (2009). Von der Döner - Prüfung zum Döner - Heariing. <i>Fleischwirtschaft </i>, pp. 61–65.","ieee":"B. Becker, G. Hildebrandt, I. Erol, J. Kleer, and A. S. Manopas, “Von der Döner - Prüfung zum Döner - Heariing,” <i>Fleischwirtschaft </i>, no. 7, pp. 61–65, 2009.","chicago":"Becker, Barbara, Goetz Hildebrandt, Irfan  Erol, Josef Kleer, and Aranya Sira Manopas. “Von Der Döner - Prüfung Zum Döner - Heariing.” <i>Fleischwirtschaft </i>, 2009.","mla":"Becker, Barbara, et al. “Von Der Döner - Prüfung Zum Döner - Heariing.” <i>Fleischwirtschaft </i>, no. 7, 2009, pp. 61–65."},"department":[{"_id":"DEP4010"}],"year":2009,"title":"Von der Döner - Prüfung zum Döner - Heariing","issue":"7","author":[{"last_name":"Becker","full_name":"Becker, Barbara","id":"12640","first_name":"Barbara"},{"full_name":"Hildebrandt, Goetz","last_name":"Hildebrandt","first_name":"Goetz"},{"last_name":"Erol","full_name":"Erol, Irfan ","first_name":"Irfan "},{"last_name":"Kleer","full_name":"Kleer, Josef","first_name":"Josef"},{"first_name":"Aranya Sira","last_name":"Manopas","full_name":"Manopas, Aranya Sira"}],"status":"public","type":"newspaper_article"},{"user_id":"83779","_id":"6128","date_updated":"2024-05-23T11:12:12Z","language":[{"iso":"eng"}],"citation":{"ieee":"K. Schwarzer, B. Becker, J. Schneider, and U. Müller, “Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung,” presented at the ProcessNet Fachausschuss Lebensmittelverfahrenstechnik, Lausanne, 2009.","apa":"Schwarzer, K., Becker, B., Schneider, J., &#38; Müller, U. (2009). <i>Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung</i>. ProcessNet Fachausschuss Lebensmittelverfahrenstechnik, Lausanne.","havard":"K. Schwarzer, B. Becker, J. Schneider, U. Müller, Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: 2009.","chicago-de":"Schwarzer, Knut, Barbara Becker, Jan Schneider und Ulrich Müller. 2009. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: .","mla":"Schwarzer, Knut, et al. <i>Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung</i>. 2009.","chicago":"Schwarzer, Knut, Barbara Becker, Jan Schneider, and Ulrich Müller. “Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung,” 2009.","short":"K. Schwarzer, B. Becker, J. Schneider, U. Müller, in: 2009.","ufg":"<b>Schwarzer, Knut u. a.</b>: Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: o. Hg.: o. O. 2009.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Schwarzer, Knut</span> ; <span style=\"font-variant:small-caps;\">Becker, Barbara</span> ; <span style=\"font-variant:small-caps;\">Schneider, Jan</span> ; <span style=\"font-variant:small-caps;\">Müller, Ulrich</span>: Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: , 2009","bjps":"<b>Schwarzer K <i>et al.</i></b> (2009) Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung.","ama":"Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: ; 2009.","van":"Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In 2009."},"date_created":"2021-09-06T10:43:56Z","conference":{"name":"ProcessNet Fachausschuss Lebensmittelverfahrenstechnik","location":"Lausanne","start_date":"2009-03-23","end_date":"2009-03-25"},"title":"Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung","status":"public","author":[{"first_name":"Knut","full_name":"Schwarzer, Knut","id":"13329","last_name":"Schwarzer"},{"id":"12640","full_name":"Becker, Barbara","last_name":"Becker","first_name":"Barbara"},{"first_name":"Jan","last_name":"Schneider","orcid":"0000-0001-6401-8873","id":"13209","full_name":"Schneider, Jan"},{"first_name":"Ulrich","last_name":"Müller","id":"12119","full_name":"Müller, Ulrich"}],"type":"conference_abstract","department":[{"_id":"DEP4023"},{"_id":"DEP4009"},{"_id":"DEP4010"},{"_id":"DEP4018"}],"year":"2009"},{"year":"2009","department":[{"_id":"DEP4023"},{"_id":"DEP4009"},{"_id":"DEP4010"},{"_id":"DEP4018"}],"type":"conference_abstract","author":[{"last_name":"Schwarzer","full_name":"Schwarzer, Knut","id":"13329","first_name":"Knut"},{"last_name":"Becker","id":"12640","full_name":"Becker, Barbara","first_name":"Barbara"},{"first_name":"Jan","orcid":"0000-0001-6401-8873","last_name":"Schneider","full_name":"Schneider, Jan","id":"13209"},{"first_name":"Ulrich","id":"12119","full_name":"Müller, Ulrich","last_name":"Müller"}],"status":"public","title":"Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung","conference":{"start_date":"2009-10-22","end_date":"2009-10-24","name":"GDL-Kongress ","location":"Lemgo"},"date_created":"2021-09-06T11:10:55Z","citation":{"ufg":"<b>Schwarzer, Knut u. a.</b>: Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: o. Hg.: o. O. 2009.","ieee":"K. Schwarzer, B. Becker, J. Schneider, and U. Müller, “Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung,” presented at the GDL-Kongress , Lemgo, 2009.","short":"K. Schwarzer, B. Becker, J. Schneider, U. Müller, in: 2009.","chicago-de":"Schwarzer, Knut, Barbara Becker, Jan Schneider und Ulrich Müller. 2009. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: .","havard":"K. Schwarzer, B. Becker, J. Schneider, U. Müller, Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: 2009.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Schwarzer, Knut</span> ; <span style=\"font-variant:small-caps;\">Becker, Barbara</span> ; <span style=\"font-variant:small-caps;\">Schneider, Jan</span> ; <span style=\"font-variant:small-caps;\">Müller, Ulrich</span>: Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: , 2009","apa":"Schwarzer, K., Becker, B., Schneider, J., &#38; Müller, U. (2009). <i>Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung</i>. GDL-Kongress , Lemgo.","bjps":"<b>Schwarzer K <i>et al.</i></b> (2009) Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung.","chicago":"Schwarzer, Knut, Barbara Becker, Jan Schneider, and Ulrich Müller. “Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung,” 2009.","van":"Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In 2009.","ama":"Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der Entkeimung. In: ; 2009.","mla":"Schwarzer, Knut, et al. <i>Die Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung</i>. 2009."},"language":[{"iso":"eng"}],"date_updated":"2024-05-23T11:12:44Z","_id":"6141","user_id":"83779"},{"intvolume":"        76","publication":"  Applied and environmental microbiology / American Society for Microbiology","doi":"10.1128/aem.01797-09","publisher":"American Society for Microbiology","language":[{"iso":"eng"}],"citation":{"ieee":"S. Mormann, M. Dabisch-Ruthe, and B. Becker, “Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods,” <i>  Applied and environmental microbiology / American Society for Microbiology</i>, vol. 76, no. 2, pp. 536–545, 2009, doi: <a href=\"https://doi.org/10.1128/aem.01797-09\">10.1128/aem.01797-09</a>.","ufg":"<b>Mormann, Sascha/Dabisch-Ruthe, Mareike/Becker, Barbara</b>: Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods, in: <i>  Applied and environmental microbiology / American Society for Microbiology</i> 76 (2009), H. 2,  S. 536–545.","short":"S. Mormann, M. Dabisch-Ruthe, B. Becker,   Applied and Environmental Microbiology / American Society for Microbiology 76 (2009) 536–545.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Mormann, Sascha</span> ; <span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Becker, Barbara</span>: Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. In: <i>  Applied and environmental microbiology / American Society for Microbiology</i> Bd. 76. Washington, DC [u.a.], American Society for Microbiology (2009), Nr. 2, S. 536–545","havard":"S. Mormann, M. Dabisch-Ruthe, B. Becker, Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods,   Applied and Environmental Microbiology / American Society for Microbiology. 76 (2009) 536–545.","chicago-de":"Mormann, Sascha, Mareike Dabisch-Ruthe und Barbara Becker. 2009. Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. <i>  Applied and environmental microbiology / American Society for Microbiology</i> 76, Nr. 2: 536–545. doi:<a href=\"https://doi.org/10.1128/aem.01797-09\">10.1128/aem.01797-09</a>, .","apa":"Mormann, S., Dabisch-Ruthe, M., &#38; Becker, B. (2009). Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. <i>  Applied and Environmental Microbiology / American Society for Microbiology</i>, <i>76</i>(2), 536–545. <a href=\"https://doi.org/10.1128/aem.01797-09\">https://doi.org/10.1128/aem.01797-09</a>","bjps":"<b>Mormann S, Dabisch-Ruthe M and Becker B</b> (2009) Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. <i>  Applied and environmental microbiology / American Society for Microbiology</i> <b>76</b>, 536–545.","chicago":"Mormann, Sascha, Mareike Dabisch-Ruthe, and Barbara Becker. “Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods.” <i>  Applied and Environmental Microbiology / American Society for Microbiology</i> 76, no. 2 (2009): 536–45. <a href=\"https://doi.org/10.1128/aem.01797-09\">https://doi.org/10.1128/aem.01797-09</a>.","mla":"Mormann, Sascha, et al. “Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods.” <i>  Applied and Environmental Microbiology / American Society for Microbiology</i>, vol. 76, no. 2, 2009, pp. 536–45, <a href=\"https://doi.org/10.1128/aem.01797-09\">https://doi.org/10.1128/aem.01797-09</a>.","ama":"Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods. <i>  Applied and environmental microbiology / American Society for Microbiology</i>. 2009;76(2):536-545. doi:<a href=\"https://doi.org/10.1128/aem.01797-09\">10.1128/aem.01797-09</a>","van":"Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods.   Applied and environmental microbiology / American Society for Microbiology. 2009;76(2):536–45."},"page":"536-545","user_id":"83781","_id":"12982","status":"public","quality_controlled":"1","date_created":"2025-06-17T06:26:59Z","publication_identifier":{"issn":["0099-2240 "],"eissn":["1098-5336"]},"date_updated":"2025-06-17T13:49:35Z","volume":76,"publication_status":"published","year":"2009","abstract":[{"text":"Contaminated food is a significant vehicle for human norovirus transmission. The present study determined the effect of physicochemical treatments on the tenacity of infective human norovirus genogroup II in selected foods. Artificially contaminated produce was subjected to a number of processes used by the food industry for preservation and by the consumer for storage and preparation. Virus recovery was carried out by using ultrafiltration and was monitored by using bacteriophage MS2 as an internal process control. Norovirus was quantified by using monoplex one-step TaqMan real-time reverse transcription (RT)-PCR and an external standard curve based on recombinant RNA standards. An RNase pretreatment step was used to avoid false-positive PCR results caused by accessible RNA, which allowed detection of intact virus particles. Significant reductions in titers were obtained with heat treatments usually applied by consumers for food preparation (baking, cooking, roasting). Generally, processes used for preservation and storage, such as cooling, freezing, acidification (≥pH 4.5), and moderate heat treatments (pasteurization), appear to be insufficient to inactivate norovirus within a food matrix or on the surface of food. Besides data for persistence in processed food, comparable data for individual matrix-specific protective effects, recovery rates, and inhibitory effects on the PCRs were obtained in this study. The established procedure might be used for other noncultivable enteric RNA viruses that are connected to food-borne diseases. The data obtained in this study may also help optimize the process for inactivation of norovirus in food by adjusting food processing technologies and may promote the development of risk assessment systems in order to improve consumer protection.","lang":"eng"}],"department":[{"_id":"DEP4010"}],"place":"Washington, DC [u.a.]","type":"scientific_journal_article","issue":"2","title":"Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods","author":[{"first_name":"Sascha","last_name":"Mormann","full_name":"Mormann, Sascha"},{"first_name":"Mareike","last_name":"Dabisch-Ruthe","orcid":"https://orcid.org/0009-0008-7644-0826","full_name":"Dabisch-Ruthe, Mareike","id":"66516"},{"full_name":"Becker, Barbara","id":"12640","last_name":"Becker","first_name":"Barbara"}]},{"date_created":"2020-05-18T09:33:00Z","citation":{"bjps":"<b>Becker B</b> (2004) Lebensmittelassoziierte Viren - Aktuelle Apekte.","chicago":"Becker, Barbara. “Lebensmittelassoziierte Viren - Aktuelle Apekte,” 2004.","van":"Becker B. Lebensmittelassoziierte Viren - aktuelle Apekte. In 2004.","ama":"Becker B. Lebensmittelassoziierte Viren - aktuelle Apekte. In: ; 2004.","mla":"Becker, Barbara. <i>Lebensmittelassoziierte Viren - Aktuelle Apekte</i>. 2004.","ufg":"<b>Becker, Barbara (2004)</b>: Lebensmittelassoziierte Viren - aktuelle Apekte, in: .","ieee":"B. Becker, “Lebensmittelassoziierte Viren - aktuelle Apekte,” presented at the Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie, 2004.","short":"B. Becker, in: 2004.","chicago-de":"Becker, Barbara. 2004. Lebensmittelassoziierte Viren - aktuelle Apekte. In: .","havard":"B. Becker, Lebensmittelassoziierte Viren - aktuelle Apekte, in: 2004.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Becker, Barbara</span>: Lebensmittelassoziierte Viren - aktuelle Apekte. In: , 2004","apa":"Becker, B. (2004). Lebensmittelassoziierte Viren - aktuelle Apekte. Presented at the Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie."},"language":[{"iso":"eng"}],"date_updated":"2023-03-15T13:49:39Z","user_id":"74222","_id":"2359","year":2004,"department":[{"_id":"DEP4010"}],"type":"conference","status":"public","author":[{"id":"12640","full_name":"Becker, Barbara","last_name":"Becker","first_name":"Barbara"}],"title":"Lebensmittelassoziierte Viren - aktuelle Apekte","conference":{"name":"Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie","start_date":"14.07.2004","end_date":"16.07.2004"}}]