---
_id: '12955'
abstract:
- lang: ger
text: Ein 10 Monate alter, männlich intakter Rhodesian Ridgeback wurde wegen chronischen
Dickdarmdurchfalls und Hämatochezie vorgestellt. Der Hund stammte aus Deutschland
und hatte das Land nie verlassen. Die Laboruntersuchung des vorbehandelnden Tierarztes
ergab neben einer Neutrophilie eine Hyperkaliämie und eine Hyponatriämie. Mit
einem Serumbasalkortisolwert von 4,3 µg/dl konnte ein Hypoadrenokortizismus weitgehend
ausgeschlossen werden. Eine vom Tierarzt durch geführte antibiotische Behandlung
hatte keine Besserung bewirkt. Daher war der Hund mit Prednisolon behandelt worden.
Unter 2-wöchiger Prednisolongabe kam es zu einer deutlichen Verstärkung des Durchfalls
sowie einem Gewichtsverlust von 6 kg. Bei Vorstellung an der Medizinischen Kleintierklinik
der LMU München war der Hund im Allgemeinbefinden mittelgradig reduziert, deutlich
abgemagert, dehydriert, hypovolämisch und hatte eine rektale Körpertemperatur
von 39,6 °C. Bei der sonografischen Untersuchung zeigte sich eine generalisiert
verdickte Dickdarmwand und koloskopisch eine hochgradig ulzerativ veränderte Dickdarmschleimhaut.
Histologischer Befund war eine ulzerative granulomatöse Kolitis. Durch die Periodic-Acid-Schiff-Reaktion
ließen sich in den Schnitten der Dickdarmbioptate mikrobielle Strukturen darstellen,
die für eine Algeninfektion diagnostisch waren. Die bei der mikrobiellen Untersuchung
anzüchtbaren Prototheken wurden mittels MALDI-TOF-MS als Prototheca zopfii identifiziert.
Zum Nachweis einer möglichen Immundefizienz wurden die Immunglobuline im Serum
bestimmt. Die IgM-Konzentration war erniedrigt, während sich IgG- und IgA-Konzentration
im Referenzbereich befanden. Aufgrund der Verschlechterung des Allgemeinbefindens,
der vorsichtigen Prognose und der hohen Kosten eines Therapieversuchs wurde der
Hund eine Woche später euthanasiert und der Tierkörper pathologisch untersucht.
Histopathologisch wurden Prototheken auch in den abdominalen Lymphknoten, jedoch
nicht in den Augen oder im zentralen Nervensystem identifiziert. Der Fall zeigt,
dass eine Prototheken-Infektion auch bei Hunden aus Deutschland als Differenzialdiagnose
für chronischen Dickdarmdurchfall in Betracht gezogen werden sollte, insbesondere
bei Patienten mit ulzerativer granulomatöser Kolitis. Sie kann bei der histologischen
Untersuchung ohne Spezialfärbung leicht übersehen werden.
- lang: eng
text: A 10-month-old male Rhodesian Ridgeback was presented to the Clinic of Small
Animal Medicine, LMU, Germany, with a 6-month history of chronic diarrhea and
hematochezia. The dog lived in Germany and had never traveled abroad. Complete
blood count and serum biochemistry performed by the referring veterinarian revealed
neutrophilia, hyperkalemia, and hyponatremia, with a basal cortisol of 4.3 µg/dl,
which excluded hypoadrenocorticism. Since antibiotic treatment had not resulted
in any improvement, a 2 week course of prednisolone administration had been initiated,
leading to a marked deterioration of intestinal signs and a significant weight
loss of 6 kg. At the time of referral, the patient was markedly emaciated, dehydrated,
hypovolemic and had a rectal temperature of 39.6 °C. Abdominal ultrasound showed
a thickened and irregular colonic wall. On colonoscopy, an irregular colonic mucosa
with ulcerations was observed. Histopathologic examination revealed an ulcerative
granulomatous colitis, and on Periodic acid-Schiff reaction (PAS) numerous organisms
consistent with Prototheca spp. were identified. Prototheca zopfii infection was
confirmed by culture and MALDI-TOF MS. In order to test for an underlying immunodeficiency,
immunoglobulin levels in serum were determined. IgM was decreased, while IgG and
IgA levels were within the reference interval. Due to deterioration of general
condition, grave prognosis and costs of a treatment trial, the patient was euthanized
one week later, and necropsy was performed. Prototheca spp. were detected on histopathologic
examination in the lymphnodes, however not in the eyes or the central nervous
system. Protothecosis should be considered an differential diagnosis in dogs with
chronic diarrhea and ulcerative granulomatous colitis even in dogs living in Germany.
Histopathologic examination of colonic biopsies with special stains such as PAS
is recommended in every dog with signs of chronic large bowel disease in order
to avoid missing this rare infectious disease.
author:
- first_name: Vera
full_name: Geisen, Vera
last_name: Geisen
- first_name: Christian
full_name: Mayer, Christian
last_name: Mayer
- first_name: Julia
full_name: Harrer, Julia
last_name: Harrer
- first_name: Katrin
full_name: Hartmann, Katrin
last_name: Hartmann
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Stefan
full_name: Unterer, Stefan
last_name: Unterer
citation:
ama: 'Geisen V, Mayer C, Harrer J, Hartmann K, Ulrich S, Unterer S. Ulzerative granulomatöse
Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche
Praxis Ausgabe K: Kleintiere / Heimtiere. 2020;48(05):369-375. doi:10.1055/a-1238-1554'
apa: 'Geisen, V., Mayer, C., Harrer, J., Hartmann, K., Ulrich, S., & Unterer,
S. (2020). Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian
Ridgeback in Deutschland. Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere,
48(05), 369–375. https://doi.org/10.1055/a-1238-1554'
bjps: 'Geisen V et al. (2020) Ulzerative granulomatöse Kolitis durch
Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche
Praxis Ausgabe K: Kleintiere / Heimtiere 48, 369–375.'
chicago: 'Geisen, Vera, Christian Mayer, Julia Harrer, Katrin Hartmann, Sebastian
Ulrich, and Stefan Unterer. “Ulzerative granulomatöse Kolitis durch Prototheca
spp. bei einem Rhodesian Ridgeback in Deutschland.” Tierärztliche Praxis Ausgabe
K: Kleintiere / Heimtiere 48, no. 05 (2020): 369–75. https://doi.org/10.1055/a-1238-1554.'
chicago-de: 'Geisen, Vera, Christian Mayer, Julia Harrer, Katrin Hartmann, Sebastian
Ulrich und Stefan Unterer. 2020. Ulzerative granulomatöse Kolitis durch Prototheca
spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche Praxis Ausgabe
K: Kleintiere / Heimtiere 48, Nr. 05: 369–375. doi:10.1055/a-1238-1554,
.'
din1505-2-1: 'Geisen, Vera ; Mayer, Christian ; Harrer,
Julia ; Hartmann, Katrin
; Ulrich, Sebastian ; Unterer,
Stefan: Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem
Rhodesian Ridgeback in Deutschland. In: Tierärztliche Praxis Ausgabe K: Kleintiere
/ Heimtiere Bd. 48. Stuttgart, Thieme (2020), Nr. 05, S. 369–375'
havard: 'V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, S. Unterer, Ulzerative
granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland,
Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere. 48 (2020) 369–375.'
ieee: 'V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, and S. Unterer, “Ulzerative
granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland,”
Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, vol. 48, no. 05,
pp. 369–375, 2020, doi: 10.1055/a-1238-1554.'
mla: 'Geisen, Vera, et al. “Ulzerative granulomatöse Kolitis durch Prototheca spp.
bei einem Rhodesian Ridgeback in Deutschland.” Tierärztliche Praxis Ausgabe
K: Kleintiere / Heimtiere, vol. 48, no. 05, 2020, pp. 369–75, https://doi.org/10.1055/a-1238-1554.'
short: 'V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, S. Unterer, Tierärztliche
Praxis Ausgabe K: Kleintiere / Heimtiere 48 (2020) 369–375.'
ufg: 'Geisen, Vera u. a.: Ulzerative granulomatöse Kolitis durch Prototheca
spp. bei einem Rhodesian Ridgeback in Deutschland, in: Tierärztliche Praxis
Ausgabe K: Kleintiere / Heimtiere 48 (2020), H. 05, S. 369–375.'
van: 'Geisen V, Mayer C, Harrer J, Hartmann K, Ulrich S, Unterer S. Ulzerative granulomatöse
Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche
Praxis Ausgabe K: Kleintiere / Heimtiere. 2020;48(05):369–75.'
date_created: 2025-06-15T10:01:12Z
date_updated: 2025-06-18T12:24:09Z
department:
- _id: DEP4010
doi: 10.1055/a-1238-1554
extern: '1'
intvolume: ' 48'
issue: '05'
keyword:
- Hund - Algeninfektion - chronischer Durchfall - Protothekose - Prototheca zopfii
language:
- iso: ger
page: 369-375
place: Stuttgart
publication: 'Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere'
publication_identifier:
eissn:
- 2567-5842
issn:
- 1434-1239
publication_status: published
publisher: 'Thieme '
status: public
title: Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian
Ridgeback in Deutschland
type: scientific_journal_article
user_id: '83781'
volume: 48
year: '2020'
...
---
_id: '12956'
abstract:
- lang: eng
text: Stachybotrys (S.) chartarum had been linked to severe health problems in humans
and animals, which occur after exposure to the toxic secondary metabolites of
this mold. S. chartarum had been isolated from different environmental sources,
ranging from culinary herbs and improperly stored fodder to damp building materials.
To access the pathogenic potential of isolates, it is essential to analyze them
under defined conditions that allow for the production of their toxic metabolites.
All Stachybotrys species are assumed to produce the immunosuppressive phenylspirodrimanes,
but the highly cytotoxic macrocyclic trichothecenes are exclusively generated
by the genotype S of S. chartarum. In this study, we have analyzed four genotype
S strains initially isolated from three different habitats. We grew them on five
commonly used media (malt-extract-agar, glucose-yeast-peptone-agar, potato-dextrose-agar,
cellulose-agar, Sabouraud-dextrose-agar) to identify conditions that promote mycotoxin
production. Using LC-MS/MS, we have quantified stachybotrylactam and all S-type
specific macrocyclic trichothecenes (satratoxin G, H, F, roridin E, L-2, verrucarin
J). All five media supported a comparable fungal growth and sporulation at 25
°C in the dark. The highest concentrations of macrocyclic trichothecenes were
detected on potato-dextrose-agar or cellulose-agar. Malt-extract-agar let to an
intermediate and glucose-yeast-peptone-agar and Sabouraud-dextrose-agar to a poor
mycotoxin production. These data demonstrate that the mycotoxin production clearly
depends on the composition of the respective medium. Our findings provide a starting
point for further studies in order to identify individual components that either
support or repress the production of mycotoxins in S. chartarum.
article_number: '159'
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Cornelius
full_name: Schäfer, Cornelius
last_name: Schäfer
citation:
ama: Ulrich S, Schäfer C. Toxin Production by Stachybotrys chartarum Genotype S
on Different Culture Media. Journal of Fungi. 2020;6(3). doi:10.3390/jof6030159
apa: Ulrich, S., & Schäfer, C. (2020). Toxin Production by Stachybotrys chartarum
Genotype S on Different Culture Media. Journal of Fungi, 6(3), Article
159. https://doi.org/10.3390/jof6030159
bjps: Ulrich S and Schäfer C (2020) Toxin Production by Stachybotrys Chartarum
Genotype S on Different Culture Media. Journal of Fungi 6.
chicago: Ulrich, Sebastian, and Cornelius Schäfer. “Toxin Production by Stachybotrys
Chartarum Genotype S on Different Culture Media.” Journal of Fungi 6, no.
3 (2020). https://doi.org/10.3390/jof6030159.
chicago-de: Ulrich, Sebastian und Cornelius Schäfer. 2020. Toxin Production by Stachybotrys
chartarum Genotype S on Different Culture Media. Journal of Fungi 6, Nr.
3. doi:10.3390/jof6030159, .
din1505-2-1: 'Ulrich, Sebastian ;
Schäfer, Cornelius: Toxin Production
by Stachybotrys chartarum Genotype S on Different Culture Media. In: Journal
of Fungi Bd. 6. Basel, MDPI (2020), Nr. 3'
havard: S. Ulrich, C. Schäfer, Toxin Production by Stachybotrys chartarum Genotype
S on Different Culture Media, Journal of Fungi. 6 (2020).
ieee: 'S. Ulrich and C. Schäfer, “Toxin Production by Stachybotrys chartarum Genotype
S on Different Culture Media,” Journal of Fungi, vol. 6, no. 3, Art. no.
159, 2020, doi: 10.3390/jof6030159.'
mla: Ulrich, Sebastian, and Cornelius Schäfer. “Toxin Production by Stachybotrys
Chartarum Genotype S on Different Culture Media.” Journal of Fungi, vol.
6, no. 3, 159, 2020, https://doi.org/10.3390/jof6030159.
short: S. Ulrich, C. Schäfer, Journal of Fungi 6 (2020).
ufg: 'Ulrich, Sebastian/Schäfer, Cornelius: Toxin Production by Stachybotrys
chartarum Genotype S on Different Culture Media, in: Journal of Fungi 6
(2020), H. 3.'
van: Ulrich S, Schäfer C. Toxin Production by Stachybotrys chartarum Genotype S
on Different Culture Media. Journal of Fungi. 2020;6(3).
date_created: 2025-06-15T10:01:42Z
date_updated: 2025-06-16T13:59:56Z
department:
- _id: DEP4010
doi: 10.3390/jof6030159
extern: '1'
intvolume: ' 6'
issue: '3'
keyword:
- Stachybotrys
- genotype
- macrocyclic trichothecenes
- stachybotrylactam
language:
- iso: eng
place: Basel
publication: Journal of Fungi
publication_identifier:
eissn:
- 2309-608X
publication_status: published
publisher: 'MDPI '
quality_controlled: '1'
status: public
title: Toxin Production by Stachybotrys chartarum Genotype S on Different Culture
Media
type: scientific_journal_article
user_id: '83781'
volume: 6
year: '2020'
...
---
_id: '12962'
abstract:
- lang: eng
text: "Background\r\nBorrelia burgdorferi is a tick-borne spirochete that causes
Lyme borreliosis (LB). After an initial tick bite, it spreads from the deposition
site in the dermis to distant tissues of the host. It is generally believed that
this spirochete disseminates via the hematogenous route. Borrelia persica causes
relapsing fever and is able to replicate in the blood stream. Currently the exact
dissemination pathway of LB pathogens in the host is not known and controversially
discussed.\r\nMethods\r\nIn this study, we established a strict intravenous infection
murine model using host-adapted spirochetes. Survival capacity and infectivity
of host-adapted B. burgdorferi sensu stricto (Bbss) were compared to those of
B. persica (Bp) after either intradermal (ID) injection into the dorsal skin of
immunocompetent mice or strict intravenous (IV) inoculation via the jugular vein.
By in vitro culture and PCR, viable spirochetes and their DNA load in peripheral
blood were periodically monitored during a 49/50-day course post-injection, as
well as in various tissue samples collected at day 49/50. Specific antibodies
in individual plasma/serum samples were detected with serological methods.\r\nResults\r\nRegardless
of ID or IV injection, DNA of Bp was present in blood samples up to day 24 post-challenge,
while no Bbss was detectable in the blood circulation during the complete observation
period. In contrast to the brain tropism of Bp, Bbss spirochetes were found in
ear, skin, joint, bladder, and heart tissue samples of only ID-inoculated mice.
All tested tissues collected from IV-challenged mice were negative for traces
of Bbss. ELISA testing of serum samples showed that Bp induced gradually increasing
antibody levels after ID or IV inoculation, while Bbss did so only after ID injection
but not after IV inoculation.\r\nConclusions\r\nThis study allows us to draw the
following conclusions: (i) Bp survives in the blood and disseminates to the host’s
brain via the hematogenous route; and (ii) Bbss, in contrast, is cleared rapidly
from the blood stream and is a tissue-bound spirochete."
article_number: '191'
author:
- first_name: Liucun
full_name: Liang, Liucun
last_name: Liang
- first_name: Jinyong
full_name: Wang, Jinyong
last_name: Wang
- first_name: Lucas
full_name: Schorter, Lucas
last_name: Schorter
- first_name: Thu Phong
full_name: Nguyen Trong, Thu Phong
last_name: Nguyen Trong
- first_name: Shari
full_name: Fell, Shari
last_name: Fell
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Reinhard K.
full_name: Straubinger, Reinhard K.
last_name: Straubinger
citation:
ama: Liang L, Wang J, Schorter L, et al. Rapid clearance of Borrelia burgdorferi
from the blood circulation. Parasites & vectors. 2020;13(1). doi:10.1186/s13071-020-04060-y
apa: Liang, L., Wang, J., Schorter, L., Nguyen Trong, T. P., Fell, S., Ulrich, S.,
& Straubinger, R. K. (2020). Rapid clearance of Borrelia burgdorferi from
the blood circulation. Parasites & Vectors, 13(1), Article 191.
https://doi.org/10.1186/s13071-020-04060-y
bjps: Liang L et al. (2020) Rapid Clearance of Borrelia Burgdorferi
from the Blood Circulation. Parasites & vectors 13.
chicago: Liang, Liucun, Jinyong Wang, Lucas Schorter, Thu Phong Nguyen Trong, Shari
Fell, Sebastian Ulrich, and Reinhard K. Straubinger. “Rapid Clearance of Borrelia
Burgdorferi from the Blood Circulation.” Parasites & Vectors 13, no.
1 (2020). https://doi.org/10.1186/s13071-020-04060-y.
chicago-de: Liang, Liucun, Jinyong Wang, Lucas Schorter, Thu Phong Nguyen Trong,
Shari Fell, Sebastian Ulrich und Reinhard K. Straubinger. 2020. Rapid clearance
of Borrelia burgdorferi from the blood circulation. Parasites & vectors
13, Nr. 1. doi:10.1186/s13071-020-04060-y,
.
din1505-2-1: 'Liang, Liucun ; Wang, Jinyong ; Schorter,
Lucas ; Nguyen Trong, Thu Phong
; Fell, Shari ; Ulrich,
Sebastian ; Straubinger, Reinhard
K.: Rapid clearance of Borrelia burgdorferi from the blood circulation.
In: Parasites & vectors Bd. 13. London, BioMed Central (2020), Nr. 1'
havard: L. Liang, J. Wang, L. Schorter, T.P. Nguyen Trong, S. Fell, S. Ulrich, R.K.
Straubinger, Rapid clearance of Borrelia burgdorferi from the blood circulation,
Parasites & Vectors. 13 (2020).
ieee: 'L. Liang et al., “Rapid clearance of Borrelia burgdorferi from the
blood circulation,” Parasites & vectors, vol. 13, no. 1, Art. no. 191,
2020, doi: 10.1186/s13071-020-04060-y.'
mla: Liang, Liucun, et al. “Rapid Clearance of Borrelia Burgdorferi from the Blood
Circulation.” Parasites & Vectors, vol. 13, no. 1, 191, 2020, https://doi.org/10.1186/s13071-020-04060-y.
short: L. Liang, J. Wang, L. Schorter, T.P. Nguyen Trong, S. Fell, S. Ulrich, R.K.
Straubinger, Parasites & Vectors 13 (2020).
ufg: 'Liang, Liucun u. a.: Rapid clearance of Borrelia burgdorferi from the
blood circulation, in: Parasites & vectors 13 (2020), H. 1.'
van: Liang L, Wang J, Schorter L, Nguyen Trong TP, Fell S, Ulrich S, et al. Rapid
clearance of Borrelia burgdorferi from the blood circulation. Parasites &
vectors. 2020;13(1).
date_created: 2025-06-15T10:13:30Z
date_updated: 2025-06-17T14:17:27Z
department:
- _id: DEP4010
doi: 10.1186/s13071-020-04060-y
extern: '1'
intvolume: ' 13'
issue: '1'
keyword:
- Lyme borreliosis
- Borrelia burgdorferi
- Tick-borne relapsing fever
- Borrelia persica
- Blood clearance
language:
- iso: eng
place: London
publication: Parasites & vectors
publication_identifier:
issn:
- 1756-3305
publication_status: published
publisher: 'BioMed Central '
quality_controlled: '1'
status: public
title: Rapid clearance of Borrelia burgdorferi from the blood circulation
type: scientific_journal_article
user_id: '83781'
volume: 13
year: '2020'
...
---
_id: '12963'
abstract:
- lang: eng
text: The genus Borrelia comprises vector-borne bacterial pathogens that can severely
affect human and animal health. Members of the Borrelia burgdorferi sensu lato
species complex can cause Lyme borreliosis, one of the most common vector-borne
diseases in the Northern hemisphere. Besides, members of the relapsing fever group
of spirochetes can cause tick-borne relapsing fever in humans and various febrile
illnesses in animals in tropical, subtropical and temperate regions. Borrelia
spp. organisms are fastidious to cultivate and to maintain in vitro, and therefore,
difficult to work with in the laboratory. Currently, borrelia identification is
mainly performed using PCR and DNA sequencing methods, which can be complicated/frustrating
on complex DNA templates and may still be relatively expensive. Alternative techniques
such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF MS) are not well established for Borrelia spp., although this technique
is currently one of the most used techniques for rapid identification of bacteria
in microbiological diagnostic laboratories. This is mainly due to unsatisfactory
results obtained by use of simple sample preparation techniques and medium-contamination
obscuring the mass spectra. In addition, comprehensive libraries for Borrelia
spp. MALDI-TOF MS have yet to be established. In this study, we developed a new
filter-based chemical extraction technique that allows measurement of high quality
Borrelia spp. spectra from less than 100,000 bacteria per spot in MALDI-TOF MS.
We used 49 isolates of 13 different species to produce the largest mass-library
for Borrelia spp. so far and to validate the protocol. The library was successfully
established and identifies >96% of used isolates correctly to species level. Cluster
analysis on the sum spectra was applied to all the different isolates, which resulted
in tight cluster generation for most species. Comparative analysis of the generated
cluster to a phylogeny based on concatenated multi-locus sequence typing genes
provided a surprising homology. Our data demonstrate that the technique described
here can be used for fast and reliable species and strain typing within the borrelia
complex.
author:
- first_name: Anna-Cathrine
full_name: Neumann-Cip, Anna-Cathrine
last_name: Neumann-Cip
- first_name: Volker
full_name: Fingerle, Volker
last_name: Fingerle
- first_name: Gabriele
full_name: Margos, Gabriele
last_name: Margos
- first_name: Reinhard K.
full_name: Straubinger, Reinhard K.
last_name: Straubinger
- first_name: Evelyn
full_name: Overzier, Evelyn
last_name: Overzier
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Andreas
full_name: Wieser, Andreas
last_name: Wieser
citation:
ama: Neumann-Cip AC, Fingerle V, Margos G, et al. A Novel Rapid Sample Preparation
Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate
Differentiation. Frontiers in Microbiology. 2020;11. doi:10.3389/fmicb.2020.00690
apa: Neumann-Cip, A.-C., Fingerle, V., Margos, G., Straubinger, R. K., Overzier,
E., Ulrich, S., & Wieser, A. (2020). A Novel Rapid Sample Preparation Method
for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation.
Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.00690
bjps: Neumann-Cip A-C et al. (2020) A Novel Rapid Sample Preparation
Method for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate
Differentiation. Frontiers in Microbiology 11.
chicago: Neumann-Cip, Anna-Cathrine, Volker Fingerle, Gabriele Margos, Reinhard
K. Straubinger, Evelyn Overzier, Sebastian Ulrich, and Andreas Wieser. “A Novel
Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia Burgdorferi
Sensu Lato Species and Isolate Differentiation.” Frontiers in Microbiology
11 (2020). https://doi.org/10.3389/fmicb.2020.00690.
chicago-de: Neumann-Cip, Anna-Cathrine, Volker Fingerle, Gabriele Margos, Reinhard
K. Straubinger, Evelyn Overzier, Sebastian Ulrich und Andreas Wieser. 2020. A
Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi
Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology
11. doi:10.3389/fmicb.2020.00690,
.
din1505-2-1: 'Neumann-Cip, Anna-Cathrine
; Fingerle, Volker ; Margos,
Gabriele ; Straubinger, Reinhard
K. ; Overzier, Evelyn ; Ulrich, Sebastian ; Wieser,
Andreas: A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits
Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation. In: Frontiers
in Microbiology Bd. 11. Lausanne, Frontiers Media SA (2020)'
havard: A.-C. Neumann-Cip, V. Fingerle, G. Margos, R.K. Straubinger, E. Overzier,
S. Ulrich, A. Wieser, A Novel Rapid Sample Preparation Method for MALDI-TOF MS
Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation, Frontiers
in Microbiology. 11 (2020).
ieee: 'A.-C. Neumann-Cip et al., “A Novel Rapid Sample Preparation Method
for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation,”
Frontiers in Microbiology, vol. 11, 2020, doi: 10.3389/fmicb.2020.00690.'
mla: Neumann-Cip, Anna-Cathrine, et al. “A Novel Rapid Sample Preparation Method
for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate Differentiation.”
Frontiers in Microbiology, vol. 11, 2020, https://doi.org/10.3389/fmicb.2020.00690.
short: A.-C. Neumann-Cip, V. Fingerle, G. Margos, R.K. Straubinger, E. Overzier,
S. Ulrich, A. Wieser, Frontiers in Microbiology 11 (2020).
ufg: 'Neumann-Cip, Anna-Cathrine u. a.: A Novel Rapid Sample Preparation
Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate
Differentiation, in: Frontiers in Microbiology 11 (2020).'
van: Neumann-Cip AC, Fingerle V, Margos G, Straubinger RK, Overzier E, Ulrich S,
et al. A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia
burgdorferi Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology.
2020;11.
date_created: 2025-06-15T10:15:09Z
date_updated: 2025-06-18T11:09:40Z
department:
- _id: DEP4010
doi: 10.3389/fmicb.2020.00690
extern: '1'
intvolume: ' 11'
keyword:
- Borrelia burgdorferi sensu lato
- MALDI-TOF MS
- typing
- sample preparation
- MALDI-TOF MS library
- strain typing
- automatic identification
language:
- iso: eng
place: Lausanne
publication: Frontiers in Microbiology
publication_identifier:
eissn:
- 1664-302X
publication_status: published
publisher: Frontiers Media SA
quality_controlled: '1'
status: public
title: A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi
Sensu Lato Species and Isolate Differentiation
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2020'
...
---
_id: '12964'
abstract:
- lang: eng
text: Antibodies represent an important element in the adaptive immune response
and a major tool to eliminate microbial pathogens. For many bacterial and viral
infections, efficient vaccines exist, but not for fungal pathogens. For a long
time, antibodies have been assumed to be of minor importance for a successful
clearance of fungal infections; however this perception has been challenged by
a large number of studies over the last three decades. In this review, we focus
on the potential therapeutic and prophylactic use of monoclonal antibodies. Since
systemic mycoses normally occur in severely immunocompromised patients, a passive
immunization using monoclonal antibodies is a promising approach to directly attack
the fungal pathogen and/or to activate and strengthen the residual antifungal
immune response in these patients.
article_number: '22'
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Frank
full_name: Ebel, Frank
last_name: Ebel
citation:
ama: Ulrich S, Ebel F. Monoclonal Antibodies as Tools to Combat Fungal Infections.
Journal of Fungi. 2020;6(1). doi:10.3390/jof6010022
apa: Ulrich, S., & Ebel, F. (2020). Monoclonal Antibodies as Tools to Combat
Fungal Infections. Journal of Fungi, 6(1), Article 22. https://doi.org/10.3390/jof6010022
bjps: Ulrich S and Ebel F (2020) Monoclonal Antibodies as Tools to Combat
Fungal Infections. Journal of Fungi 6.
chicago: Ulrich, Sebastian, and Frank Ebel. “Monoclonal Antibodies as Tools to Combat
Fungal Infections.” Journal of Fungi 6, no. 1 (2020). https://doi.org/10.3390/jof6010022.
chicago-de: Ulrich, Sebastian und Frank Ebel. 2020. Monoclonal Antibodies as Tools
to Combat Fungal Infections. Journal of Fungi 6, Nr. 1. doi:10.3390/jof6010022,
.
din1505-2-1: 'Ulrich, Sebastian ;
Ebel, Frank: Monoclonal Antibodies
as Tools to Combat Fungal Infections. In: Journal of Fungi Bd. 6. Basel,
MDPI (2020), Nr. 1'
havard: S. Ulrich, F. Ebel, Monoclonal Antibodies as Tools to Combat Fungal Infections,
Journal of Fungi. 6 (2020).
ieee: 'S. Ulrich and F. Ebel, “Monoclonal Antibodies as Tools to Combat Fungal Infections,”
Journal of Fungi, vol. 6, no. 1, Art. no. 22, 2020, doi: 10.3390/jof6010022.'
mla: Ulrich, Sebastian, and Frank Ebel. “Monoclonal Antibodies as Tools to Combat
Fungal Infections.” Journal of Fungi, vol. 6, no. 1, 22, 2020, https://doi.org/10.3390/jof6010022.
short: S. Ulrich, F. Ebel, Journal of Fungi 6 (2020).
ufg: 'Ulrich, Sebastian/Ebel, Frank: Monoclonal Antibodies as Tools to Combat
Fungal Infections, in: Journal of Fungi 6 (2020), H. 1.'
van: Ulrich S, Ebel F. Monoclonal Antibodies as Tools to Combat Fungal Infections.
Journal of Fungi. 2020;6(1).
date_created: 2025-06-15T10:17:00Z
date_updated: 2025-06-18T11:13:40Z
department:
- _id: DEP4010
doi: 10.3390/jof6010022
extern: '1'
intvolume: ' 6'
issue: '1'
keyword:
- monoclonal antibodies
- invasive fungal infections
- therapy
- prophylaxis
- opsonization
language:
- iso: eng
place: Basel
publication: Journal of Fungi
publication_identifier:
eissn:
- 2309-608X
publication_status: published
publisher: 'MDPI '
quality_controlled: '1'
status: public
title: Monoclonal Antibodies as Tools to Combat Fungal Infections
type: scientific_journal_article
user_id: '83781'
volume: 6
year: '2020'
...
---
_id: '12965'
abstract:
- lang: eng
text: "The Bacillus (B.) cereus group consists of nine recognized species which
are present worldwide. B. cereus play an important role in food-borne diseases
by producing different toxins. Yet, only a small percentage of B. cereus strains
are able to produce the heat stable cereulide, the causative agent of emetic food
poisoning. To minimize the entry of emetic B. cereus into the food chain, food
business operators are dependent on efficient and reliable methods enabling the
differentiation between emetic and non-emetic strains.\r\nCurrently, only time-consuming
cell bioassays, molecular methods and tandem mass spectrometry are available for
this purpose. Thus, the aim of the present study was to establish a fast and reliable
method for the differentiation between emetic/non-emetic strains by MALDI-TOF
MS. Selected strains/isolates of the B. cereus group as well as other Bacillus
spp. (total n = 121) were cultured on sheep blood agar for 48 h before analysis.\r\nSubsequently,
the cultures were directly analyzed by MALDI-TOF MS without prior extraction steps.
The samples were measured in the mass range of m/z 800–1800 Da. Using ClinProTools
3.0 statistical software and Flex analysis software (Bruker Daltonics GmbH, Bremen,
Germany), a differentiation between emetic/non-emetic isolates was possible with
a rate of correct identification of 99.1% by means of the evaluation of two specific
biomarkers (m/z 1171 and 1187 Da)."
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Christoph
full_name: Gottschalk, Christoph
last_name: Gottschalk
- first_name: Richard
full_name: Dietrich, Richard
last_name: Dietrich
- first_name: Erwin
full_name: Märtlbauer, Erwin
last_name: Märtlbauer
- first_name: Manfred
full_name: Gareis, Manfred
last_name: Gareis
citation:
ama: Ulrich S, Gottschalk C, Dietrich R, Märtlbauer E, Gareis M. Identification
of cereulide producing Bacillus cereus by MALDI-TOF MS. Food Microbiology.
2019;82:75-81. doi:10.1016/j.fm.2019.01.012
apa: Ulrich, S., Gottschalk, C., Dietrich, R., Märtlbauer, E., & Gareis, M.
(2019). Identification of cereulide producing Bacillus cereus by MALDI-TOF MS.
Food Microbiology, 82, 75–81. https://doi.org/10.1016/j.fm.2019.01.012
bjps: Ulrich S et al. (2019) Identification of Cereulide Producing
Bacillus Cereus by MALDI-TOF MS. Food Microbiology 82, 75–81.
chicago: 'Ulrich, Sebastian, Christoph Gottschalk, Richard Dietrich, Erwin Märtlbauer,
and Manfred Gareis. “Identification of Cereulide Producing Bacillus Cereus by
MALDI-TOF MS.” Food Microbiology 82 (2019): 75–81. https://doi.org/10.1016/j.fm.2019.01.012.'
chicago-de: 'Ulrich, Sebastian, Christoph Gottschalk, Richard Dietrich, Erwin Märtlbauer
und Manfred Gareis. 2019. Identification of cereulide producing Bacillus cereus
by MALDI-TOF MS. Food Microbiology 82: 75–81. doi:10.1016/j.fm.2019.01.012,
.'
din1505-2-1: 'Ulrich, Sebastian ;
Gottschalk, Christoph ; Dietrich,
Richard ; Märtlbauer, Erwin
; Gareis, Manfred: Identification
of cereulide producing Bacillus cereus by MALDI-TOF MS. In: Food Microbiology
Bd. 82. London, Academic Press (2019), S. 75–81'
havard: S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, M. Gareis, Identification
of cereulide producing Bacillus cereus by MALDI-TOF MS, Food Microbiology. 82
(2019) 75–81.
ieee: 'S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, and M. Gareis, “Identification
of cereulide producing Bacillus cereus by MALDI-TOF MS,” Food Microbiology,
vol. 82, pp. 75–81, 2019, doi: 10.1016/j.fm.2019.01.012.'
mla: Ulrich, Sebastian, et al. “Identification of Cereulide Producing Bacillus Cereus
by MALDI-TOF MS.” Food Microbiology, vol. 82, 2019, pp. 75–81, https://doi.org/10.1016/j.fm.2019.01.012.
short: S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, M. Gareis, Food Microbiology
82 (2019) 75–81.
ufg: 'Ulrich, Sebastian u. a.: Identification of cereulide producing Bacillus
cereus by MALDI-TOF MS, in: Food Microbiology 82 (2019), S. 75–81.'
van: Ulrich S, Gottschalk C, Dietrich R, Märtlbauer E, Gareis M. Identification
of cereulide producing Bacillus cereus by MALDI-TOF MS. Food Microbiology. 2019;82:75–81.
date_created: 2025-06-15T10:18:20Z
date_updated: 2025-06-18T11:16:35Z
department:
- _id: DEP4010
doi: 10.1016/j.fm.2019.01.012
extern: '1'
intvolume: ' 82'
keyword:
- MALDI-TOF MS
- Bacillus cereus
- Cereulide
- Food intoxication
language:
- iso: eng
page: 75-81
place: London
publication: Food Microbiology
publication_identifier:
eissn:
- 1095-9998
issn:
- 0740-0020
publication_status: published
publisher: 'Academic Press '
quality_controlled: '1'
status: public
title: Identification of cereulide producing Bacillus cereus by MALDI-TOF MS
type: scientific_journal_article
user_id: '83781'
volume: 82
year: '2019'
...
---
_id: '12966'
abstract:
- lang: eng
text: 'The fungus Stachybotrys (S.) chartarum was isolated from culinary herbs,
damp building materials, and improperly stored animal forage. Two distinct chemotypes
of the fungus were described that produced either high-cytotoxic macrocyclic trichothecenes
(S type) or low-cytotoxic atranones (A type). Recently, two distinct gene clusters
were described that were found to be necessary for the biosynthesis of either
macrocyclic trichothecenes (21 SAT (Satratoxin) genes) or atranones (14 ATR (Atranone)
genes). In the current study, PCR primers were designed to detect SAT and ATR
genes in 19 S. chartarum chemotype S and eight S. chartarum chemotype A strains.
Our analysis revealed the existence of three different genotypes: satratoxin-producing
strains that harbored all SAT genes but lacked the ATR gene cluster (genotype
S), non-satratoxin-producing strains that possessed the ATR genes but lacked SAT
genes (genotype A), and a hitherto undescribed hybrid genotype among non-satratoxin-producing
strains that harbored all ATR genes and an incomplete set of SAT genes (genotype
H). In order to improve the discrimination of genotypes, a triplex PCR assay was
developed and applied for the analysis of S. chartarum and S. chlorohalonata cultures.
The results show that genes for macrocyclic trichothecenes and atranones are not
mutually exclusive in S. chartarum. Correlation of the new genotype-based concept
with mycotoxin production data shows also that macrocyclic trichothecenes are
exclusively produced by S. chartarum genotype S strains.'
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Ludwig
full_name: Niessen, Ludwig
last_name: Niessen
- first_name: Julia
full_name: Ekruth, Julia
last_name: Ekruth
- first_name: Cornelius
full_name: Schäfer, Cornelius
last_name: Schäfer
- first_name: Florian
full_name: Kaltner, Florian
last_name: Kaltner
- first_name: Christoph
full_name: Gottschalk, Christoph
last_name: Gottschalk
citation:
ama: Ulrich S, Niessen L, Ekruth J, Schäfer C, Kaltner F, Gottschalk C. Truncated
satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys
chartarum (Ehrenb.) S. Hughes. Mycotoxin Research. 2019;36(1):83-91. doi:10.1007/s12550-019-00371-x
apa: Ulrich, S., Niessen, L., Ekruth, J., Schäfer, C., Kaltner, F., & Gottschalk,
C. (2019). Truncated satratoxin gene clusters in selected isolates of the atranone
chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. Mycotoxin Research,
36(1), 83–91. https://doi.org/10.1007/s12550-019-00371-x
bjps: Ulrich S et al. (2019) Truncated Satratoxin Gene Clusters in
Selected Isolates of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.)
S. Hughes. Mycotoxin Research 36, 83–91.
chicago: 'Ulrich, Sebastian, Ludwig Niessen, Julia Ekruth, Cornelius Schäfer, Florian
Kaltner, and Christoph Gottschalk. “Truncated Satratoxin Gene Clusters in Selected
Isolates of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.) S. Hughes.”
Mycotoxin Research 36, no. 1 (2019): 83–91. https://doi.org/10.1007/s12550-019-00371-x.'
chicago-de: 'Ulrich, Sebastian, Ludwig Niessen, Julia Ekruth, Cornelius Schäfer,
Florian Kaltner und Christoph Gottschalk. 2019. Truncated satratoxin gene clusters
in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.)
S. Hughes. Mycotoxin Research 36, Nr. 1: 83–91. doi:10.1007/s12550-019-00371-x,
.'
din1505-2-1: 'Ulrich, Sebastian ;
Niessen, Ludwig ; Ekruth,
Julia ; Schäfer, Cornelius
; Kaltner, Florian ; Gottschalk,
Christoph: Truncated satratoxin gene clusters in selected isolates of the
atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. In: Mycotoxin
Research Bd. 36. Berlin ; Heidelberg, Springer (2019), Nr. 1, S. 83–91'
havard: S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, C. Gottschalk,
Truncated satratoxin gene clusters in selected isolates of the atranone chemotype
of Stachybotrys chartarum (Ehrenb.) S. Hughes, Mycotoxin Research. 36 (2019) 83–91.
ieee: 'S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, and C. Gottschalk,
“Truncated satratoxin gene clusters in selected isolates of the atranone chemotype
of Stachybotrys chartarum (Ehrenb.) S. Hughes,” Mycotoxin Research, vol.
36, no. 1, pp. 83–91, 2019, doi: 10.1007/s12550-019-00371-x.'
mla: Ulrich, Sebastian, et al. “Truncated Satratoxin Gene Clusters in Selected Isolates
of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.) S. Hughes.” Mycotoxin
Research, vol. 36, no. 1, 2019, pp. 83–91, https://doi.org/10.1007/s12550-019-00371-x.
short: S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, C. Gottschalk,
Mycotoxin Research 36 (2019) 83–91.
ufg: 'Ulrich, Sebastian u. a.: Truncated satratoxin gene clusters in selected
isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes,
in: Mycotoxin Research 36 (2019), H. 1, S. 83–91.'
van: Ulrich S, Niessen L, Ekruth J, Schäfer C, Kaltner F, Gottschalk C. Truncated
satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys
chartarum (Ehrenb.) S. Hughes. Mycotoxin Research. 2019;36(1):83–91.
date_created: 2025-06-15T10:19:53Z
date_updated: 2025-06-18T11:20:11Z
department:
- _id: DEP4010
doi: 10.1007/s12550-019-00371-x
extern: '1'
intvolume: ' 36'
issue: '1'
keyword:
- Acetyltransferases
- Chlamydomonas reinhardtii
- Fungal Genes
- Fungal genetics
- Fungal genomics
- Saccharomyces cerevisiae
language:
- iso: eng
page: 83-91
place: Berlin ; Heidelberg
publication: Mycotoxin Research
publication_identifier:
eissn:
- 1867-1632
issn:
- 0178-7888
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: Truncated satratoxin gene clusters in selected isolates of the atranone chemotype
of Stachybotrys chartarum (Ehrenb.) S. Hughes
type: scientific_journal_article
user_id: '83781'
volume: 36
year: '2019'
...
---
_id: '12967'
abstract:
- lang: eng
text: "Objectives\r\nTo evaluate matrix-assisted laser desorption ionisation time
of flight mass spectrometry (MALDI-TOF MS) combined with the Sepsityper kit (Bruker
Daltoniks GmbH, Bremen) for the direct detection of bacterial species from inoculated
blood cultures from dogs and cats.\r\nMaterials and Methods\r\nCanine and feline
blood samples were inoculated with typical sepsis-causing bacteria such as Staphylococcus
intermedius, Staphylococcus aureus, Streptococcus canis, Enterococcus faecalis,
Escherichia coli and Pseudomonas aeruginosa at two distinct concentrations (each
in triplicate), resulting in 72 blood culture bottles incubated at 37°C. Samples
were comparatively analysed with MALDI-TOF MS after preparation with the Sepsityper
kit and also by standard bacteriology (culturing and biochemical characterisation).\r\nResults\r\nBacterial
species identified from agar plates and by MALDI-TOF MS from blood culture bottles
were identical for all samples. The MALDI Biotyper software (Bruker Daltoniks)
correctly identified all bacterial strains from inoculated canine and feline blood
with analysis indicating very good precision.\r\nClinical Significance\r\nMALDI-TOF
MS analysis combined with the Sepsityper kit is a reliable tool for a quick detection
of veterinary-relevant bacterial species directly from blood culture bottles.
This approach could reduce the time for identification of critical species to
only 24 hours."
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: C.
full_name: Gottschalk, C.
last_name: Gottschalk
- first_name: R. Kk
full_name: Straubinger, R. Kk
last_name: Straubinger
- first_name: K.
full_name: Schwaiger, K.
last_name: Schwaiger
- first_name: R.
full_name: Dörfelt, R.
last_name: Dörfelt
citation:
ama: Ulrich S, Gottschalk C, Straubinger RK, Schwaiger K, Dörfelt R. Acceleration
of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood.
Journal of Small Animal Practice. 2019;61(1):42-45. doi:10.1111/jsap.13056
apa: Ulrich, S., Gottschalk, C., Straubinger, R. K., Schwaiger, K., & Dörfelt,
R. (2019). Acceleration of the identification of sepsis‐inducing bacteria in cultures
of dog and cat blood. Journal of Small Animal Practice, 61(1), 42–45.
https://doi.org/10.1111/jsap.13056
bjps: Ulrich S et al. (2019) Acceleration of the Identification of
Sepsis‐inducing Bacteria in Cultures of Dog and Cat Blood. Journal of Small
Animal Practice 61, 42–45.
chicago: 'Ulrich, Sebastian, C. Gottschalk, R. Kk Straubinger, K. Schwaiger, and
R. Dörfelt. “Acceleration of the Identification of Sepsis‐inducing Bacteria in
Cultures of Dog and Cat Blood.” Journal of Small Animal Practice 61, no.
1 (2019): 42–45. https://doi.org/10.1111/jsap.13056.'
chicago-de: 'Ulrich, Sebastian, C. Gottschalk, R. Kk Straubinger, K. Schwaiger und
R. Dörfelt. 2019. Acceleration of the identification of sepsis‐inducing bacteria
in cultures of dog and cat blood. Journal of Small Animal Practice 61,
Nr. 1: 42–45. doi:10.1111/jsap.13056,
.'
din1505-2-1: 'Ulrich, Sebastian ;
Gottschalk, C. ; Straubinger,
R. Kk ; Schwaiger, K. ; Dörfelt, R.: Acceleration of the identification
of sepsis‐inducing bacteria in cultures of dog and cat blood. In: Journal of
Small Animal Practice Bd. 61. Oxford [u.a.], Wiley-Blackwell (2019), Nr. 1,
S. 42–45'
havard: S. Ulrich, C. Gottschalk, R.K. Straubinger, K. Schwaiger, R. Dörfelt, Acceleration
of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood,
Journal of Small Animal Practice. 61 (2019) 42–45.
ieee: 'S. Ulrich, C. Gottschalk, R. K. Straubinger, K. Schwaiger, and R. Dörfelt,
“Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog
and cat blood,” Journal of Small Animal Practice, vol. 61, no. 1, pp. 42–45,
2019, doi: 10.1111/jsap.13056.'
mla: Ulrich, Sebastian, et al. “Acceleration of the Identification of Sepsis‐inducing
Bacteria in Cultures of Dog and Cat Blood.” Journal of Small Animal Practice,
vol. 61, no. 1, 2019, pp. 42–45, https://doi.org/10.1111/jsap.13056.
short: S. Ulrich, C. Gottschalk, R.K. Straubinger, K. Schwaiger, R. Dörfelt, Journal
of Small Animal Practice 61 (2019) 42–45.
ufg: 'Ulrich, Sebastian u. a.: Acceleration of the identification of sepsis‐inducing
bacteria in cultures of dog and cat blood, in: Journal of Small Animal Practice
61 (2019), H. 1, S. 42–45.'
van: Ulrich S, Gottschalk C, Straubinger RK, Schwaiger K, Dörfelt R. Acceleration
of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood.
Journal of Small Animal Practice. 2019;61(1):42–5.
date_created: 2025-06-15T10:20:55Z
date_updated: 2025-06-18T11:24:12Z
department:
- _id: DEP4010
doi: 10.1111/jsap.13056
extern: '1'
intvolume: ' 61'
issue: '1'
language:
- iso: eng
page: 42-45
place: Oxford [u.a.]
publication: Journal of Small Animal Practice
publication_identifier:
issn:
- 0022-4510
- 1748-5827
publication_status: published
publisher: Wiley-Blackwell
quality_controlled: '1'
status: public
title: Acceleration of the identification of sepsis‐inducing bacteria in cultures
of dog and cat blood
type: scientific_journal_article
user_id: '83781'
volume: 61
year: '2019'
...
---
_id: '12971'
abstract:
- lang: eng
text: The genus Stachybotrys produces a broad diversity of secondary metabolites,
including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although
the class of the phenylspirodrimanes is the major one and consists of a multitude
of metabolites bearing various structural modifications, few investigations have
been carried out. Thus, the presented study deals with the quantitative determination
of several secondary metabolites produced by distinct Stachybotrys species for
comparison of their metabolite profiles. For that purpose, 15 of the primarily
produced secondary metabolites were isolated from fungal cultures and structurally
characterized in order to be used as analytical standards for the development
of an LC-MS/MS multimethod. The developed method was applied to the analysis of
micro-scale extracts from 5 different Stachybotrys strains, which were cultured
on different media. In that process, spontaneous dialdehyde/lactone isomerization
was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes
were quantitatively investigated for the first time. The metabolite profiles of
Stachybotrys species are considerably influenced by time of growth and substrate
availability, as well as the individual biosynthetic potential of the respective
species. Regarding the reported adverse effects associated with Stachybotrys growth
in building environments, combinatory effects of the investigated secondary metabolites
should be addressed and the role of the phenylspirodrimanes re-evaluated in future
research.
article_number: '133'
author:
- first_name: Annika
full_name: Jagels, Annika
last_name: Jagels
- first_name: Viktoria
full_name: Lindemann, Viktoria
last_name: Lindemann
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Christoph
full_name: Gottschalk, Christoph
last_name: Gottschalk
- first_name: Benedikt
full_name: Cramer, Benedikt
last_name: Cramer
- first_name: Florian
full_name: Hübner, Florian
last_name: Hübner
- first_name: Manfred
full_name: Gareis, Manfred
last_name: Gareis
- first_name: Hans-Ulrich
full_name: Humpf, Hans-Ulrich
last_name: Humpf
citation:
ama: Jagels A, Lindemann V, Ulrich S, et al. Exploring Secondary Metabolite Profiles
of Stachybotrys spp. by LC-MS/MS. Toxins. 2019;11(3). doi:10.3390/toxins11030133
apa: Jagels, A., Lindemann, V., Ulrich, S., Gottschalk, C., Cramer, B., Hübner,
F., Gareis, M., & Humpf, H.-U. (2019). Exploring Secondary Metabolite Profiles
of Stachybotrys spp. by LC-MS/MS. Toxins, 11(3), Article 133. https://doi.org/10.3390/toxins11030133
bjps: Jagels A et al. (2019) Exploring Secondary Metabolite Profiles
of Stachybotrys Spp. by LC-MS/MS. Toxins 11.
chicago: Jagels, Annika, Viktoria Lindemann, Sebastian Ulrich, Christoph Gottschalk,
Benedikt Cramer, Florian Hübner, Manfred Gareis, and Hans-Ulrich Humpf. “Exploring
Secondary Metabolite Profiles of Stachybotrys Spp. by LC-MS/MS.” Toxins
11, no. 3 (2019). https://doi.org/10.3390/toxins11030133.
chicago-de: Jagels, Annika, Viktoria Lindemann, Sebastian Ulrich, Christoph Gottschalk,
Benedikt Cramer, Florian Hübner, Manfred Gareis und Hans-Ulrich Humpf. 2019. Exploring
Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. Toxins
11, Nr. 3. doi:10.3390/toxins11030133,
.
din1505-2-1: 'Jagels, Annika ; Lindemann, Viktoria ; Ulrich,
Sebastian ; Gottschalk, Christoph
; Cramer, Benedikt ; Hübner,
Florian ; Gareis, Manfred
; Humpf, Hans-Ulrich: Exploring
Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. In: Toxins
Bd. 11. Basel, MDPI (2019), Nr. 3'
havard: A. Jagels, V. Lindemann, S. Ulrich, C. Gottschalk, B. Cramer, F. Hübner,
M. Gareis, H.-U. Humpf, Exploring Secondary Metabolite Profiles of Stachybotrys
spp. by LC-MS/MS, Toxins. 11 (2019).
ieee: 'A. Jagels et al., “Exploring Secondary Metabolite Profiles of Stachybotrys
spp. by LC-MS/MS,” Toxins, vol. 11, no. 3, Art. no. 133, 2019, doi: 10.3390/toxins11030133.'
mla: Jagels, Annika, et al. “Exploring Secondary Metabolite Profiles of Stachybotrys
Spp. by LC-MS/MS.” Toxins, vol. 11, no. 3, 133, 2019, https://doi.org/10.3390/toxins11030133.
short: A. Jagels, V. Lindemann, S. Ulrich, C. Gottschalk, B. Cramer, F. Hübner,
M. Gareis, H.-U. Humpf, Toxins 11 (2019).
ufg: 'Jagels, Annika u. a.: Exploring Secondary Metabolite Profiles of Stachybotrys
spp. by LC-MS/MS, in: Toxins 11 (2019), H. 3.'
van: Jagels A, Lindemann V, Ulrich S, Gottschalk C, Cramer B, Hübner F, et al. Exploring
Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. Toxins. 2019;11(3).
date_created: 2025-06-15T10:23:36Z
date_updated: 2025-06-17T14:12:55Z
department:
- _id: DEP4010
doi: 10.3390/toxins11030133
extern: '1'
intvolume: ' 11'
issue: '3'
keyword:
- Stachybotrys spp.
- metabolite profiles
- LC-MS/MS
- satratoxins
- phenylspirodrimanes
- stachybotrychromenes
- biosynthetic production
language:
- iso: eng
place: Basel
publication: Toxins
publication_identifier:
eissn:
- 2072-6651
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2019'
...
---
_id: '12973'
abstract:
- lang: eng
text: 'Psychrophilic and psychrotolerant clostridia (n = 110) were isolated from
vacuum-packed meat (beef and lamb), fresh venison and from skin and fecal samples
of wild boars. They were identified to species level using MALDI-TOF MS, sequence
and phylogeny analysis of the 16S rRNA and species specific multiplex qPCR. The
results of all three methods were concordant. The majority of isolates were identified
as C. tagluense-like Group I (n = 34) and Group II (n = 42). Thirty-five isolates
could be identified to species level as follows: C. estertheticum (n = 15), C.
frigoriphilum (n = 13), C. frigidicarnis (n = 1) and C. bowmanii (n = 5). This
is the first report of detection and identification of C. frigoriphilum and C.
tagluense-like Group II as causative agents of blown pack spoilage of beef. The
species specific multiplex qPCR developed in this study could be applied to identify
and to quantify the Clostridium species described above in suspicious meat juice
samples.'
author:
- first_name: Samart
full_name: Dorn-In, Samart
last_name: Dorn-In
- first_name: Karin
full_name: Schwaiger, Karin
last_name: Schwaiger
- first_name: Claudia
full_name: Springer, Claudia
last_name: Springer
- first_name: Leonard
full_name: Barta, Leonard
last_name: Barta
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Manfred
full_name: Gareis, Manfred
last_name: Gareis
citation:
ama: Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development
of a multiplex qPCR for the species identification of Clostridium estertheticum,
C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
meats and from wild boars. International Journal of Food Microbiology.
2018;286:162-169. doi:10.1016/j.ijfoodmicro.2018.08.020
apa: Dorn-In, S., Schwaiger, K., Springer, C., Barta, L., Ulrich, S., & Gareis,
M. (2018). Development of a multiplex qPCR for the species identification of Clostridium
estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown
pack spoilage (BPS) meats and from wild boars. International Journal of Food
Microbiology, 286, 162–169. https://doi.org/10.1016/j.ijfoodmicro.2018.08.020
bjps: Dorn-In S et al. (2018) Development of a Multiplex QPCR for
the Species Identification of Clostridium Estertheticum, C. Frigoriphilum, C.
Bowmanii and C. Tagluense-like from Blown Pack Spoilage (BPS) Meats and from Wild
Boars. International Journal of Food Microbiology 286, 162–169.
chicago: 'Dorn-In, Samart, Karin Schwaiger, Claudia Springer, Leonard Barta, Sebastian
Ulrich, and Manfred Gareis. “Development of a Multiplex QPCR for the Species Identification
of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like
from Blown Pack Spoilage (BPS) Meats and from Wild Boars.” International Journal
of Food Microbiology 286 (2018): 162–69. https://doi.org/10.1016/j.ijfoodmicro.2018.08.020.'
chicago-de: 'Dorn-In, Samart, Karin Schwaiger, Claudia Springer, Leonard Barta,
Sebastian Ulrich und Manfred Gareis. 2018. Development of a multiplex qPCR for
the species identification of Clostridium estertheticum, C. frigoriphilum, C.
bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild
boars. International Journal of Food Microbiology 286: 162–169. doi:10.1016/j.ijfoodmicro.2018.08.020,
.'
din1505-2-1: 'Dorn-In, Samart ; Schwaiger, Karin ; Springer,
Claudia ; Barta, Leonard
; Ulrich, Sebastian ; Gareis,
Manfred: Development of a multiplex qPCR for the species identification
of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like
from blown pack spoilage (BPS) meats and from wild boars. In: International
Journal of Food Microbiology Bd. 286. Amsterdam, Elsevier (2018), S. 162–169'
havard: S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, M. Gareis, Development
of a multiplex qPCR for the species identification of Clostridium estertheticum,
C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
meats and from wild boars, International Journal of Food Microbiology. 286 (2018)
162–169.
ieee: 'S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, and M. Gareis,
“Development of a multiplex qPCR for the species identification of Clostridium
estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown
pack spoilage (BPS) meats and from wild boars,” International Journal of Food
Microbiology, vol. 286, pp. 162–169, 2018, doi: 10.1016/j.ijfoodmicro.2018.08.020.'
mla: Dorn-In, Samart, et al. “Development of a Multiplex QPCR for the Species Identification
of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like
from Blown Pack Spoilage (BPS) Meats and from Wild Boars.” International Journal
of Food Microbiology, vol. 286, 2018, pp. 162–69, https://doi.org/10.1016/j.ijfoodmicro.2018.08.020.
short: S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, M. Gareis, International
Journal of Food Microbiology 286 (2018) 162–169.
ufg: 'Dorn-In, Samart u. a.: Development of a multiplex qPCR for the species
identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and
C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars, in:
International Journal of Food Microbiology 286 (2018), S. 162–169.'
van: Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development
of a multiplex qPCR for the species identification of Clostridium estertheticum,
C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
meats and from wild boars. International Journal of Food Microbiology. 2018;286:162–9.
date_created: 2025-06-15T10:25:24Z
date_updated: 2025-06-18T11:27:12Z
department:
- _id: DEP4010
doi: 10.1016/j.ijfoodmicro.2018.08.020
extern: '1'
intvolume: ' 286'
keyword:
- Blown pack spoilage
- MALDI-TOF MS
- PCR
- 16S rRNA
- C. frigoriphilum
- C. tagluense-like
language:
- iso: eng
page: 162-169
place: Amsterdam
publication: International Journal of Food Microbiology
publication_identifier:
eissn:
- 1879-3460
issn:
- 0168-1605
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Development of a multiplex qPCR for the species identification of Clostridium
estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack
spoilage (BPS) meats and from wild boars
type: scientific_journal_article
user_id: '83781'
volume: 286
year: '2018'
...
---
_id: '12974'
abstract:
- lang: eng
text: "BACKGROUND\r\nFruits and vegetables have increasingly been related to foodborne
outbreaks. Besides surface contamination, a possible internalization of microorganisms
into edible parts of plants during growth has already been observed. To examine
an actual risk for the consumer, microbial contamination of the rind and pulp
of 147 muskmelons from international trade was assessed using cultural and biochemical
methods, polymerase chain reaction and matrix-assisted laser desorption/ionization-time
of flight mass spectrometry.\r\nRESULTS\r\nOne hundred percent of the rind samples
[3.69–8.92 log colony forming units (CFU) g−1] and 89.8% of the pulp samples (maximum
load 3.66 log CFU g−1) were microbiologically contaminated. Among the 432 pulp
isolates, opportunistic and potentially pathogenic bacteria were identified, mainly
Staphylococcus spp. (48.9%), Clostridium spp. (42.9%) and Enterobacteriaceae (27.9%).
Salmonella spp., Escherichia coli and isolates of the Bacillus cereus group were
found on the rind (1.4%, 0.7% and 42.9%, respectively) and in the pulp (0.7%,
1.4% and 4.7%). Clostridium perfringens was isolated from the rind of seven melons.\r\nCONCLUSION\r\nThe
present study revealed a regularly occurring internal contamination of melons.
Possible health risks for consumers because of an occurrence of microorganisms
in melon pulp should be considered in future food safety assessments. © 2018 Society
of Chemical Industry."
author:
- first_name: Irene
full_name: Esteban‐Cuesta, Irene
last_name: Esteban‐Cuesta
- first_name: Nathalie
full_name: Drees, Nathalie
last_name: Drees
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Peter
full_name: Stauch, Peter
last_name: Stauch
- first_name: Brigitte
full_name: Sperner, Brigitte
last_name: Sperner
- first_name: Karin
full_name: Schwaiger, Karin
last_name: Schwaiger
- first_name: Manfred
full_name: Gareis, Manfred
last_name: Gareis
- first_name: Christoph
full_name: Gottschalk, Christoph
last_name: Gottschalk
citation:
ama: 'Esteban‐Cuesta I, Drees N, Ulrich S, et al. Endogenous microbial contamination
of melons (Cucumis melo) from international trade: an underestimated risk
for the consumer? Journal of the science of food and agriculture : incorporating
Agri-Biotech. 2018;98(13):5074-5081. doi:10.1002/jsfa.9045'
apa: 'Esteban‐Cuesta, I., Drees, N., Ulrich, S., Stauch, P., Sperner, B., Schwaiger,
K., Gareis, M., & Gottschalk, C. (2018). Endogenous microbial contamination
of melons (Cucumis melo) from international trade: an underestimated risk
for the consumer? Journal of the Science of Food and Agriculture : Incorporating
Agri-Biotech, 98(13), 5074–5081. https://doi.org/10.1002/jsfa.9045'
bjps: 'Esteban‐Cuesta I et al. (2018) Endogenous Microbial Contamination
of Melons (Cucumis Melo) from International Trade: An Underestimated Risk
for the Consumer? Journal of the science of food and agriculture : incorporating
Agri-Biotech 98, 5074–5081.'
chicago: 'Esteban‐Cuesta, Irene, Nathalie Drees, Sebastian Ulrich, Peter Stauch,
Brigitte Sperner, Karin Schwaiger, Manfred Gareis, and Christoph Gottschalk. “Endogenous
Microbial Contamination of Melons (Cucumis Melo) from International Trade:
An Underestimated Risk for the Consumer?” Journal of the Science of Food and
Agriculture : Incorporating Agri-Biotech 98, no. 13 (2018): 5074–81. https://doi.org/10.1002/jsfa.9045.'
chicago-de: 'Esteban‐Cuesta, Irene, Nathalie Drees, Sebastian Ulrich, Peter Stauch,
Brigitte Sperner, Karin Schwaiger, Manfred Gareis und Christoph Gottschalk. 2018.
Endogenous microbial contamination of melons (Cucumis melo) from international
trade: an underestimated risk for the consumer? Journal of the science of food
and agriculture : incorporating Agri-Biotech 98, Nr. 13: 5074–5081. doi:10.1002/jsfa.9045, .'
din1505-2-1: 'Esteban‐Cuesta, Irene
; Drees, Nathalie ; Ulrich,
Sebastian ; Stauch, Peter
; Sperner, Brigitte ; Schwaiger,
Karin ; Gareis, Manfred ;
Gottschalk, Christoph: Endogenous
microbial contamination of melons (Cucumis melo) from international trade:
an underestimated risk for the consumer? In: Journal of the science of food
and agriculture : incorporating Agri-Biotech Bd. 98. Chichester, Wiley (2018),
Nr. 13, S. 5074–5081'
havard: 'I. Esteban‐Cuesta, N. Drees, S. Ulrich, P. Stauch, B. Sperner, K. Schwaiger,
M. Gareis, C. Gottschalk, Endogenous microbial contamination of melons (Cucumis
melo) from international trade: an underestimated risk for the consumer?,
Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech. 98
(2018) 5074–5081.'
ieee: 'I. Esteban‐Cuesta et al., “Endogenous microbial contamination of melons
(Cucumis melo) from international trade: an underestimated risk for the
consumer?,” Journal of the science of food and agriculture : incorporating
Agri-Biotech, vol. 98, no. 13, pp. 5074–5081, 2018, doi: 10.1002/jsfa.9045.'
mla: 'Esteban‐Cuesta, Irene, et al. “Endogenous Microbial Contamination of Melons
(Cucumis Melo) from International Trade: An Underestimated Risk for the
Consumer?” Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech,
vol. 98, no. 13, 2018, pp. 5074–81, https://doi.org/10.1002/jsfa.9045.'
short: 'I. Esteban‐Cuesta, N. Drees, S. Ulrich, P. Stauch, B. Sperner, K. Schwaiger,
M. Gareis, C. Gottschalk, Journal of the Science of Food and Agriculture : Incorporating
Agri-Biotech 98 (2018) 5074–5081.'
ufg: 'Esteban‐Cuesta, Irene u. a.: Endogenous microbial contamination of
melons (Cucumis melo) from international trade: an underestimated risk
for the consumer?, in: Journal of the science of food and agriculture : incorporating
Agri-Biotech 98 (2018), H. 13, S. 5074–5081.'
van: 'Esteban‐Cuesta I, Drees N, Ulrich S, Stauch P, Sperner B, Schwaiger K, et
al. Endogenous microbial contamination of melons (Cucumis melo) from international
trade: an underestimated risk for the consumer? Journal of the science of food
and agriculture : incorporating Agri-Biotech. 2018;98(13):5074–81.'
date_created: 2025-06-15T10:28:46Z
date_updated: 2025-06-18T11:32:30Z
department:
- _id: DEP4010
doi: 10.1002/jsfa.9045
extern: '1'
intvolume: ' 98'
issue: '13'
keyword:
- foodborne pathogens
- Salmonella
- Listeria monocytogenes
- Enterobacteriaceae
- vegetables
language:
- iso: eng
page: 5074-5081
place: Chichester
publication: 'Journal of the science of food and agriculture : incorporating Agri-Biotech'
publication_identifier:
eissn:
- 1097-0010
issn:
- 0022-5142
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: 'Endogenous microbial contamination of melons (Cucumis melo) from international
trade: an underestimated risk for the consumer?'
type: scientific_journal_article
user_id: '83781'
volume: 98
year: '2018'
...
---
_id: '12988'
abstract:
- lang: eng
text: "BACKGROUND\r\nBacterial contamination of platelet concentrates (PCs) is still
a major challenge in transfusion medicine. Different methodologic concepts and
screening strategies have been developed and investigated concerning their usability.
We evaluated the feasibility of BacT/ALERT automated culture (BacT/A, bioMérieux)
with late sampling after 3 days at the earliest.\r\nSTUDY DESIGN AND METHODS\r\nTwenty-four
bacterial strains isolated from PCs and six relevant strains from reference stocks
were spiked into apheresis-derived PCs (10-60 colony-forming units [CFU]/bag).
Sampling was performed after 3 days, and bacterial detection was investigated
using the two detection methods (BacT/A and BactiFlow [BF], bioMérieux). The maximum
time-to-result of BacT/A was set to less than 12 hours.\r\nRESULTS\r\nAll medium-
or high-pathogenic strains are capable of proliferating to high titers, and 100%
of contaminated samples were detected by BF and BacT/A (6 to ≤12 h incubation);
lower detection rates of BacT/A were obtained within 6 hours of incubation (≤6
h: 76.2-93.4%). The majority of low-pathogenic isolates are also capable of growing
in PCs (89.7%), showing a detection rate of 74.3% for BF versus 54.3% for BacT/A
(6 to ≤12 h incubation). BacT/A failed to detect bacteria within 6 hours of incubation.
Certainly, a small number of strains did not grow under PC storage conditions
and were detectable by BacT/A only with increased detection times.\r\nCONCLUSIONS\r\nLate
sampling after 3 days at the earliest, combined with reduced BacT/A incubation
following the negative-to-date concept, offer an alternative opportunity to extend
the shelf life of PCs from 4 to 5 days in Germany. The sensitivity of BacT/A with
late sampling is nearly comparable to BF; the time-to-result is considerably longer.\r\n"
author:
- first_name: Tanja
full_name: Vollmer, Tanja
last_name: Vollmer
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Melanie
full_name: Weinstock, Melanie
last_name: Weinstock
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Jens
full_name: Dreier, Jens
last_name: Dreier
citation:
ama: Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling
for automated culture to extend the platelet shelf life to 5 days in Germany.
Transfusion. 2018;58(7):1654-1664. doi:10.1111/trf.14617
apa: Vollmer, T., Dabisch-Ruthe, M., Weinstock, M., Knabbe, C., & Dreier, J.
(2018). Late sampling for automated culture to extend the platelet shelf life
to 5 days in Germany. Transfusion, 58(7), 1654–1664. https://doi.org/10.1111/trf.14617
bjps: Vollmer T et al. (2018) Late Sampling for Automated Culture
to Extend the Platelet Shelf Life to 5 Days in Germany. Transfusion 58,
1654–1664.
chicago: 'Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe,
and Jens Dreier. “Late Sampling for Automated Culture to Extend the Platelet Shelf
Life to 5 Days in Germany.” Transfusion 58, no. 7 (2018): 1654–64. https://doi.org/10.1111/trf.14617.'
chicago-de: 'Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius
Knabbe und Jens Dreier. 2018. Late sampling for automated culture to extend the
platelet shelf life to 5 days in Germany. Transfusion 58, Nr. 7: 1654–1664.
doi:10.1111/trf.14617, .'
din1505-2-1: 'Vollmer, Tanja ; Dabisch-Ruthe, Mareike ; Weinstock,
Melanie ; Knabbe, Cornelius
; Dreier, Jens: Late sampling for
automated culture to extend the platelet shelf life to 5 days in Germany. In:
Transfusion Bd. 58. Oxford [u.a.] , Wiley-Blackwell (2018), Nr. 7, S. 1654–1664'
havard: T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Late sampling
for automated culture to extend the platelet shelf life to 5 days in Germany,
Transfusion. 58 (2018) 1654–1664.
ieee: 'T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, and J. Dreier, “Late
sampling for automated culture to extend the platelet shelf life to 5 days in
Germany,” Transfusion, vol. 58, no. 7, pp. 1654–1664, 2018, doi: 10.1111/trf.14617.'
mla: Vollmer, Tanja, et al. “Late Sampling for Automated Culture to Extend the Platelet
Shelf Life to 5 Days in Germany.” Transfusion, vol. 58, no. 7, 2018, pp.
1654–64, https://doi.org/10.1111/trf.14617.
short: T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Transfusion
58 (2018) 1654–1664.
ufg: 'Vollmer, Tanja u. a.: Late sampling for automated culture to extend
the platelet shelf life to 5 days in Germany, in: Transfusion 58 (2018),
H. 7, S. 1654–1664.'
van: Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling
for automated culture to extend the platelet shelf life to 5 days in Germany.
Transfusion. 2018;58(7):1654–64.
date_created: 2025-06-17T06:55:03Z
date_updated: 2025-06-17T13:48:11Z
department:
- _id: DEP4010
doi: 10.1111/trf.14617
intvolume: ' 58'
issue: '7'
language:
- iso: eng
page: 1654-1664
place: 'Oxford [u.a.] '
publication: Transfusion
publication_identifier:
eissn:
- 1537-2995
issn:
- 0041-1132
publication_status: published
publisher: Wiley-Blackwell
quality_controlled: '1'
status: public
title: Late sampling for automated culture to extend the platelet shelf life to 5
days in Germany
type: scientific_journal_article
user_id: '83781'
volume: 58
year: '2018'
...
---
_id: '12989'
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Melanie
full_name: Weinstock, Melanie
last_name: Weinstock
- first_name: Jens
full_name: Pfannebecker, Jens
id: '45690'
last_name: Pfannebecker
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
citation:
ama: Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Becker B. Usage of Cold Hydrogen
Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces.;
2018. doi:10.13140/RG.2.2.12883.08484
apa: Dabisch-Ruthe, M., Weinstock, M., Pfannebecker, J., & Becker, B. (2018).
Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on
fruit and vegetable surfaces. In 26th International ICFMH Conference - FoodMicro
2018. 26th International ICFMH Conference - FoodMicro 2018, Berlin. https://doi.org/10.13140/RG.2.2.12883.08484
bjps: Dabisch-Ruthe M et al. (2018) Usage of Cold Hydrogen Peroxide
Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces.
.
chicago: Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, and Barbara
Becker. Usage of Cold Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus
on Fruit and Vegetable Surfaces. 26th International ICFMH Conference -
FoodMicro 2018, 2018. https://doi.org/10.13140/RG.2.2.12883.08484.
chicago-de: Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker und Barbara
Becker. 2018. Usage of cold hydrogen peroxide vapour for inactivation of murine
norovirus on fruit and vegetable surfaces. 26th International ICFMH Conference
- FoodMicro 2018. doi:10.13140/RG.2.2.12883.08484,
.
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Weinstock, Melanie ; Pfannebecker,
Jens ; Becker, Barbara: Usage
of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit
and vegetable surfaces., 2018'
havard: M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, B. Becker, Usage of cold
hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable
surfaces., 2018.
ieee: 'M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, and B. Becker, Usage
of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit
and vegetable surfaces. 2018. doi: 10.13140/RG.2.2.12883.08484.'
mla: Dabisch-Ruthe, Mareike, et al. “Usage of Cold Hydrogen Peroxide Vapour for
Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces.” 26th International
ICFMH Conference - FoodMicro 2018, 2018, https://doi.org/10.13140/RG.2.2.12883.08484.
short: M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, B. Becker, Usage of Cold
Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable
Surfaces., 2018.
ufg: 'Dabisch-Ruthe, Mareike u. a.: Usage of cold hydrogen peroxide vapour
for inactivation of murine norovirus on fruit and vegetable surfaces., o. O. 2018.'
van: Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Becker B. Usage of cold hydrogen
peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces.
26th International ICFMH Conference - FoodMicro 2018. 2018. 482 p.
conference:
end_date: 2018-09-06
location: Berlin
name: 26th International ICFMH Conference - FoodMicro 2018
start_date: 2018--09-03
date_created: 2025-06-17T07:05:46Z
date_updated: 2025-06-17T11:49:22Z
department:
- _id: DEP4000
- _id: DEP4010
doi: 10.13140/RG.2.2.12883.08484
language:
- iso: eng
main_file_link:
- open_access: '1'
oa: '1'
page: '482'
publication: 26th International ICFMH Conference - FoodMicro 2018
publication_status: published
related_material:
link:
- relation: confirmation
url: https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00016749/26th-ICFM_FoodMicro2018_Book_of_Abstracts.pdf
status: public
title: Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus
on fruit and vegetable surfaces.
type: conference_poster
user_id: '83781'
year: '2018'
...
---
_id: '12990'
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Melanie
full_name: Weinstock, Melanie
last_name: Weinstock
- first_name: Jens
full_name: Pfannebecker, Jens
id: '45690'
last_name: Pfannebecker
- first_name: Sonja
full_name: Meyer, Sonja
id: '12829'
last_name: Meyer
- first_name: 'Mona '
full_name: 'Schwetka, Mona '
last_name: Schwetka
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
citation:
ama: Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Meyer S, Schwetka M, Becker B.
Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine
Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production. Conventus
Congressmanagement & Marketing GmbH ; 2018. doi:10.13140/RG.2.2.22949.41447
apa: Dabisch-Ruthe, M., Weinstock, M., Pfannebecker, J., Meyer, S., Schwetka, M.,
& Becker, B. (2018). Application of cold nebulized hydrogen peroxide for inactivation
of murine norovirus, bacteria and bacteria spores on surfaces in food production.
In 70th Annual Conference of the German Society of Hygiene and Microbiology.
70th Annual Conference of the German Society of Hygiene and Microbiology, Bochum.
Conventus Congressmanagement & Marketing GmbH . https://doi.org/10.13140/RG.2.2.22949.41447
bjps: Dabisch-Ruthe M et al. (2018) Application of Cold Nebulized
Hydrogen Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria
Spores on Surfaces in Food Production. Conventus Congressmanagement &
Marketing GmbH .
chicago: Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, Sonja Meyer,
Mona Schwetka, and Barbara Becker. Application of Cold Nebulized Hydrogen
Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on
Surfaces in Food Production. 70th Annual Conference of the German Society
of Hygiene and Microbiology. Conventus Congressmanagement & Marketing
GmbH , 2018. https://doi.org/10.13140/RG.2.2.22949.41447.
chicago-de: Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, Sonja
Meyer, Mona Schwetka und Barbara Becker. 2018. Application of cold nebulized
hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria
spores on surfaces in food production. 70th Annual Conference of the German
Society of Hygiene and Microbiology. Conventus Congressmanagement & Marketing
GmbH . doi:10.13140/RG.2.2.22949.41447,
.
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Weinstock, Melanie ; Pfannebecker,
Jens ; Meyer, Sonja ; Schwetka, Mona ; Becker,
Barbara: Application of cold nebulized hydrogen peroxide for inactivation
of murine norovirus, bacteria and bacteria spores on surfaces in food production. :
Conventus Congressmanagement & Marketing GmbH , 2018'
havard: M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka,
B. Becker, Application of cold nebulized hydrogen peroxide for inactivation of
murine norovirus, bacteria and bacteria spores on surfaces in food production.,
Conventus Congressmanagement & Marketing GmbH , 2018.
ieee: 'M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka, and
B. Becker, Application of cold nebulized hydrogen peroxide for inactivation
of murine norovirus, bacteria and bacteria spores on surfaces in food production.
Conventus Congressmanagement & Marketing GmbH , 2018. doi: 10.13140/RG.2.2.22949.41447.'
mla: Dabisch-Ruthe, Mareike, et al. “Application of Cold Nebulized Hydrogen Peroxide
for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on Surfaces
in Food Production.” 70th Annual Conference of the German Society of Hygiene
and Microbiology, Conventus Congressmanagement & Marketing GmbH , 2018,
https://doi.org/10.13140/RG.2.2.22949.41447.
short: M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka, B.
Becker, Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine
Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production., Conventus
Congressmanagement & Marketing GmbH , 2018.
ufg: 'Dabisch-Ruthe, Mareike u. a.: Application of cold nebulized hydrogen
peroxide for inactivation of murine norovirus, bacteria and bacteria spores on
surfaces in food production., o. O. 2018.'
van: Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Meyer S, Schwetka M, Becker B.
Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus,
bacteria and bacteria spores on surfaces in food production. 70th Annual Conference
of the German Society of Hygiene and Microbiology. Conventus Congressmanagement
& Marketing GmbH ; 2018.
conference:
end_date: 2018-02-21
location: Bochum
name: 70th Annual Conference of the German Society of Hygiene and Microbiology
start_date: 2018-02-19
date_created: 2025-06-17T07:12:33Z
date_updated: 2025-06-17T08:47:43Z
department:
- _id: DEP4010
doi: 10.13140/RG.2.2.22949.41447
language:
- iso: eng
publication: 70th Annual Conference of the German Society of Hygiene and Microbiology
publication_identifier:
isbn:
- 978-3-9816508-6-0
publication_status: published
publisher: 'Conventus Congressmanagement & Marketing GmbH '
related_material:
link:
- relation: confirmation
url: https://www.dghm.org/wp-content/uploads/2018/10/DGHM2018_Abstractband_WebVersion.pdf
status: public
title: Application of cold nebulized hydrogen peroxide for inactivation of murine
norovirus, bacteria and bacteria spores on surfaces in food production.
type: conference_scientific_abstract
user_id: '83781'
year: '2018'
...
---
_id: '12975'
abstract:
- lang: eng
text: "Properly handled fish is usually marketed as “fresh fish” until day 10 after
fishing. About 40% of the total fishery that is used for direct human consumption
is marketed in fresh form stored at temperatures up to +2 °C. Currently, there
are no validated methods available for controlling the recommended period of storage.
Apart from being a potential source for food fraud, spoiled fish represents a
major source of foodborne illnesses and intoxications.\r\nIn this study, a rapid
MALDI-TOF mass spectrometry based screening method was developed using the vitreous
fluid of fish eyes as specimen for the examination of different days of storage.
The vitreous fluid was collected from n = 100 freshly fished brown trouts at day
0, 3, 7, 9, and 11 post mortem (n = 20 brown trouts each day of examination).
The samples were immediately measured by MALDI-TOF mass spectrometry in linear
positive mode (mass range m/z 2000–20,000 Da). For quality assurance the experiment
was repeated with a set of brown trouts (n = 100) originating from the same fish
farm and with brown trouts (n = 100) originating from a different fish farm. For
specificity testing rainbow trouts (n = 10) were examined accordingly. All obtained
mass spectra were processed by means of MALDI Biotyper OC 3.1 and ClinProTools
3.0 software.\r\nThe MALDI Biotyper approach showed limited applicability for
the identification of the time of storage. However, it was suitable to reliably
discriminate between the closely related species brown and rainbow trout. Processing
by ClinProTools revealed four crucial mass peaks (m/z 2594 Da, m/z 4857 Da, m/z
4879 Da, m/z 4899 Da) which enabled a reliable differentiation between day 0 and
3, 7, 9, 11 (rate of correct identification > 90%) as well as the differentiation
between day 3 and 7, 9, 11 (rate of correct identification > 72%). However, this
approach showed limited applicability within the end of the tested period of storage
when comparing between day 7, 9, or 11."
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Philipp–Michael
full_name: Beindorf, Philipp–Michael
last_name: Beindorf
- first_name: Barbara
full_name: Biermaier, Barbara
last_name: Biermaier
- first_name: Karin
full_name: Schwaiger, Karin
last_name: Schwaiger
- first_name: Manfred
full_name: Gareis, Manfred
last_name: Gareis
- first_name: Christoph
full_name: Gottschalk, Christoph
last_name: Gottschalk
citation:
ama: Ulrich S, Beindorf P, Biermaier B, Schwaiger K, Gareis M, Gottschalk C. A novel
approach for the determination of freshness and identity of trouts by MALDI-TOF
mass spectrometry. Food Control. 2017;80(10):281-289. doi:10.1016/j.foodcont.2017.05.005
apa: Ulrich, S., Beindorf, P., Biermaier, B., Schwaiger, K., Gareis, M., & Gottschalk,
C. (2017). A novel approach for the determination of freshness and identity of
trouts by MALDI-TOF mass spectrometry. Food Control, 80(10), 281–289.
https://doi.org/10.1016/j.foodcont.2017.05.005
bjps: Ulrich S et al. (2017) A Novel Approach for the Determination
of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry. Food Control
80, 281–289.
chicago: 'Ulrich, Sebastian, Philipp–Michael Beindorf, Barbara Biermaier, Karin
Schwaiger, Manfred Gareis, and Christoph Gottschalk. “A Novel Approach for the
Determination of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry.”
Food Control 80, no. 10 (2017): 281–89. https://doi.org/10.1016/j.foodcont.2017.05.005.'
chicago-de: 'Ulrich, Sebastian, Philipp–Michael Beindorf, Barbara Biermaier, Karin
Schwaiger, Manfred Gareis und Christoph Gottschalk. 2017. A novel approach for
the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry.
Food Control 80, Nr. 10: 281–289. doi:10.1016/j.foodcont.2017.05.005,
.'
din1505-2-1: 'Ulrich, Sebastian ;
Beindorf, Philipp–Michael ; Biermaier, Barbara ; Schwaiger,
Karin ; Gareis, Manfred ;
Gottschalk, Christoph: A novel approach
for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry.
In: Food Control Bd. 80. Amsterdam, Elsevier (2017), Nr. 10, S. 281–289'
havard: S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, C. Gottschalk,
A novel approach for the determination of freshness and identity of trouts by
MALDI-TOF mass spectrometry, Food Control. 80 (2017) 281–289.
ieee: 'S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, and C. Gottschalk,
“A novel approach for the determination of freshness and identity of trouts by
MALDI-TOF mass spectrometry,” Food Control, vol. 80, no. 10, pp. 281–289,
2017, doi: 10.1016/j.foodcont.2017.05.005.'
mla: Ulrich, Sebastian, et al. “A Novel Approach for the Determination of Freshness
and Identity of Trouts by MALDI-TOF Mass Spectrometry.” Food Control, vol.
80, no. 10, 2017, pp. 281–89, https://doi.org/10.1016/j.foodcont.2017.05.005.
short: S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, C. Gottschalk,
Food Control 80 (2017) 281–289.
ufg: 'Ulrich, Sebastian u. a.: A novel approach for the determination of
freshness and identity of trouts by MALDI-TOF mass spectrometry, in: Food Control
80 (2017), H. 10, S. 281–289.'
van: Ulrich S, Beindorf P, Biermaier B, Schwaiger K, Gareis M, Gottschalk C. A novel
approach for the determination of freshness and identity of trouts by MALDI-TOF
mass spectrometry. Food Control. 2017;80(10):281–9.
date_created: 2025-06-15T10:29:56Z
date_updated: 2025-06-18T12:06:11Z
department:
- _id: DEP4010
doi: 10.1016/j.foodcont.2017.05.005
extern: '1'
intvolume: ' 80'
issue: '10'
keyword:
- MALDI-TOF
- Mass spectrometry
- Freshness
- Fish
- Quality control
- Authenticity
language:
- iso: eng
page: 281-289
place: Amsterdam
publication: Food Control
publication_identifier:
issn:
- 0956-7135
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: A novel approach for the determination of freshness and identity of trouts
by MALDI-TOF mass spectrometry
type: scientific_journal_article
user_id: '83781'
volume: 80
year: '2017'
...
---
_id: '12976'
abstract:
- lang: eng
text: "The consumption of edible insects (entomophagy) will gain greater significance
facing the increasing global population, which is suggested to reach 9 billion
people in 2050 (FAO., 2009). Due to their high amount of proteins, fatty acids,
vitamins, and minerals insects represent a valuable source of essential nutrients.\r\nWhile
the consumption of insects is very common in many countries of Africa and Asia,
there is a far smaller acceptance for entomophagy in Western cultures. Though,
products such as noodles or burger paddies made from insect meal have a better
compliance and can already be purchased in some countries of the European Union.
This processing step however involves the risk of adulteration, because there
is no more possibility to authenticate the insects once they are ground.\r\nThe
aim of this study was to investigate whether edible insects could be measured
and distinguished by MALDI-TOF MS (matrix-assisted laser desorption ionization-time
of flight mass spectrometry). Therefore, different kinds of edible insects (buffalo
worms, mealworms, crickets and grasshoppers) were purchased via online shops and
ground subsequently. The insect powder was extracted by vigorously shaking in
diluted formic acid and measured by MALDI-TOF MS. The measurement provided reproducible
as well as specific mass spectra and enabled a precise differentiation of the
different species."
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Ulrike
full_name: Kühn, Ulrike
last_name: Kühn
- first_name: Barbara
full_name: Biermaier, Barbara
last_name: Biermaier
- first_name: Nicolo
full_name: Piacenza, Nicolo
last_name: Piacenza
- first_name: Karin
full_name: Schwaiger, Karin
last_name: Schwaiger
- first_name: Christoph
full_name: Gottschalk, Christoph
last_name: Gottschalk
- first_name: Manfred
full_name: Gareis, Manfred
last_name: Gareis
citation:
ama: Ulrich S, Kühn U, Biermaier B, et al. Direct identification of edible insects
by MALDI-TOF mass spectrometry. Food Control. 2017;76(6):96-101. doi:10.1016/j.foodcont.2017.01.010
apa: Ulrich, S., Kühn, U., Biermaier, B., Piacenza, N., Schwaiger, K., Gottschalk,
C., & Gareis, M. (2017). Direct identification of edible insects by MALDI-TOF
mass spectrometry. Food Control, 76(6), 96–101. https://doi.org/10.1016/j.foodcont.2017.01.010
bjps: Ulrich S et al. (2017) Direct Identification of Edible Insects
by MALDI-TOF Mass Spectrometry. Food Control 76, 96–101.
chicago: 'Ulrich, Sebastian, Ulrike Kühn, Barbara Biermaier, Nicolo Piacenza, Karin
Schwaiger, Christoph Gottschalk, and Manfred Gareis. “Direct Identification of
Edible Insects by MALDI-TOF Mass Spectrometry.” Food Control 76, no. 6
(2017): 96–101. https://doi.org/10.1016/j.foodcont.2017.01.010.'
chicago-de: 'Ulrich, Sebastian, Ulrike Kühn, Barbara Biermaier, Nicolo Piacenza,
Karin Schwaiger, Christoph Gottschalk und Manfred Gareis. 2017. Direct identification
of edible insects by MALDI-TOF mass spectrometry. Food Control 76, Nr.
6: 96–101. doi:10.1016/j.foodcont.2017.01.010,
.'
din1505-2-1: 'Ulrich, Sebastian ;
Kühn, Ulrike ; Biermaier,
Barbara ; Piacenza, Nicolo
; Schwaiger, Karin ; Gottschalk,
Christoph ; Gareis, Manfred:
Direct identification of edible insects by MALDI-TOF mass spectrometry. In: Food
Control Bd. 76. Amsterdam, Elsevier (2017), Nr. 6, S. 96–101'
havard: S. Ulrich, U. Kühn, B. Biermaier, N. Piacenza, K. Schwaiger, C. Gottschalk,
M. Gareis, Direct identification of edible insects by MALDI-TOF mass spectrometry,
Food Control. 76 (2017) 96–101.
ieee: 'S. Ulrich et al., “Direct identification of edible insects by MALDI-TOF
mass spectrometry,” Food Control, vol. 76, no. 6, pp. 96–101, 2017, doi:
10.1016/j.foodcont.2017.01.010.'
mla: Ulrich, Sebastian, et al. “Direct Identification of Edible Insects by MALDI-TOF
Mass Spectrometry.” Food Control, vol. 76, no. 6, 2017, pp. 96–101, https://doi.org/10.1016/j.foodcont.2017.01.010.
short: S. Ulrich, U. Kühn, B. Biermaier, N. Piacenza, K. Schwaiger, C. Gottschalk,
M. Gareis, Food Control 76 (2017) 96–101.
ufg: 'Ulrich, Sebastian u. a.: Direct identification of edible insects by
MALDI-TOF mass spectrometry, in: Food Control 76 (2017), H. 6, S. 96–101.'
van: Ulrich S, Kühn U, Biermaier B, Piacenza N, Schwaiger K, Gottschalk C, et al.
Direct identification of edible insects by MALDI-TOF mass spectrometry. Food Control.
2017;76(6):96–101.
date_created: 2025-06-15T10:31:37Z
date_updated: 2025-06-18T12:08:47Z
department:
- _id: DEP4010
doi: 10.1016/j.foodcont.2017.01.010
extern: '1'
intvolume: ' 76'
issue: '6'
keyword:
- MALDI-TOF MS
- Mass spectrometry
- Edible insects
- Authenticity
- Food quality
language:
- iso: eng
page: 96-101
place: Amsterdam
publication: Food Control
publication_identifier:
issn:
- 0956-7135
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Direct identification of edible insects by MALDI-TOF mass spectrometry
type: scientific_journal_article
user_id: '83781'
volume: 76
year: '2017'
...
---
_id: '2379'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Jens
full_name: Pfannebecker, Jens
id: '45690'
last_name: Pfannebecker
citation:
ama: Becker B, Pfannebecker J. Lebensmittelassoziierte Viren - Norovirus .
1st ed. Behr`s Verlag; 2016.
apa: Becker, B., & Pfannebecker, J. (2016). Lebensmittelassoziierte Viren
- Norovirus (1st ed.). Behr`s Verlag.
bjps: Becker B and Pfannebecker J (2016) Lebensmittelassoziierte Viren
- Norovirus , 1st ed. Behr`s Verlag.
chicago: Becker, Barbara, and Jens Pfannebecker. Lebensmittelassoziierte Viren
- Norovirus . 1st ed. Lebensmittelassoziierte Viren - Norovirus . Behr`s Verlag,
2016.
chicago-de: Becker, Barbara und Jens Pfannebecker. 2016. Lebensmittelassoziierte
Viren - Norovirus . 1. Aufl. Lebensmittelassoziierte Viren - Norovirus . Behr`s
Verlag.
din1505-2-1: 'Becker, Barbara ; Pfannebecker, Jens: Lebensmittelassoziierte
Viren - Norovirus , Lebensmittelassoziierte Viren - Norovirus . 1.
Aufl. : Behr`s Verlag, 2016'
havard: B. Becker, J. Pfannebecker, Lebensmittelassoziierte Viren - Norovirus ,
1st ed., Behr`s Verlag, 2016.
ieee: B. Becker and J. Pfannebecker, Lebensmittelassoziierte Viren - Norovirus
, 1st ed. Behr`s Verlag, 2016.
mla: Becker, Barbara, and Jens Pfannebecker. Lebensmittelassoziierte Viren -
Norovirus . 1st ed., Behr`s Verlag, 2016.
short: B. Becker, J. Pfannebecker, Lebensmittelassoziierte Viren - Norovirus , 1st
ed., Behr`s Verlag, 2016.
ufg: 'Becker, Barbara/Pfannebecker, Jens (2016): Lebensmittelassoziierte
Viren - Norovirus (=Lebensmittelassoziierte Viren - Norovirus ), 1.'
van: Becker B, Pfannebecker J. Lebensmittelassoziierte Viren - Norovirus . 1st ed.
Behr`s Verlag; 2016. 80 p. (Lebensmittelassoziierte Viren - Norovirus ).
date_created: 2020-05-18T20:38:33Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4010
edition: '1'
language:
- iso: ger
page: '80'
publication_identifier:
isbn:
- 978-3-95468-388-8
publisher: Behr`s Verlag
related_material:
link:
- relation: confirmation
url: https://www.behrs.de/titel/lebensmittelassoziierte-viren-norovirus/171
series_title: 'Lebensmittelassoziierte Viren - Norovirus '
status: public
title: 'Lebensmittelassoziierte Viren - Norovirus '
type: book
user_id: '74222'
year: 2016
...
---
_id: '12977'
abstract:
- lang: eng
text: 'Stachybotrys (S.) spp. are omnipresent cellulolytic molds. Some species are
highly toxic owing to their ability to synthesize various secondary metabolites
such as macrocyclic trichothecenes or hemolysins. The reliable identification
of Stachybotrys at species level is currently limited to genome-based identification.
This study aimed to establish a fast and reliable MALDI-TOF MS identification
method by optimizing the pre-analytical steps for protein extraction for subsequent
generation of high-quality fingerprint mass spectra. Eight reference strains of
the American Type Culture Collection and the Technical University of Denmark were
cultivated in triplicate (biological repetitions) for 2 days in malt extract broth.
The mycelia (1.5 ml) were first washed with 75 % ethanol and an additional washing
step with dimethyl sulfoxide (10 %) was added to remove unspecific low weight
masses. Furthermore, mycelia were broken with roughened glass beads in formic
acid (70 %) and acetonitrile. The method was successfully applied to a total of
45 isolates of Stachybotrys originating from three different habitats (indoor,
feed, and food samples; n = 15 each): Twenty-seven isolates of S. chartarum and
18 isolates of S. chlorohalonata could be identified by MALDI-TOF MS. The data
obtained exactly matched those obtained by genome-based identification. The mean
score values for S. chartarum ranged from 2.509 to 2.739 and from 2.148 to 2.622
for S. chlorohalonata with a very good reproducibility: the relative standard
deviations were between 0.3 % and 6.8 %. Thus, MALDI-TOF MS proved to be a fast
and reliable alternative to identification of Stachybotrys spp. by nucleotide
amplification and sequencing.'
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Barbara
full_name: Biermaier, Barbara
last_name: Biermaier
- first_name: Oliver
full_name: Bader, Oliver
last_name: Bader
- first_name: Georg
full_name: Wolf, Georg
last_name: Wolf
- first_name: Reinhard K.
full_name: Straubinger, Reinhard K.
last_name: Straubinger
- first_name: Andrea
full_name: Didier, Andrea
last_name: Didier
- first_name: Brigitte
full_name: Sperner, Brigitte
last_name: Sperner
- first_name: Karin
full_name: Schwaiger, Karin
last_name: Schwaiger
- first_name: Manfred
full_name: Gareis, Manfred
last_name: Gareis
- first_name: Christoph
full_name: Gottschalk, Christoph
last_name: Gottschalk
citation:
ama: 'Ulrich S, Biermaier B, Bader O, et al. Identification of Stachybotrys spp.
by MALDI-TOF mass spectrometry. Analytical & bioanalytical chemistry :
a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica.
2016;408(27):7565-7581. doi:10.1007/s00216-016-9800-9'
apa: 'Ulrich, S., Biermaier, B., Bader, O., Wolf, G., Straubinger, R. K., Didier,
A., Sperner, B., Schwaiger, K., Gareis, M., & Gottschalk, C. (2016). Identification
of Stachybotrys spp. by MALDI-TOF mass spectrometry. Analytical & Bioanalytical
Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and
Quimica Analitica, 408(27), 7565–7581. https://doi.org/10.1007/s00216-016-9800-9'
bjps: 'Ulrich S et al. (2016) Identification of Stachybotrys Spp.
by MALDI-TOF Mass Spectrometry. Analytical & bioanalytical chemistry :
a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica
408, 7565–7581.'
chicago: 'Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard
K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis,
and Christoph Gottschalk. “Identification of Stachybotrys Spp. by MALDI-TOF Mass
Spectrometry.” Analytical & Bioanalytical Chemistry : A Merger of Fresenius’
Journal of Analytical Chemistry, Analusis and Quimica Analitica 408, no. 27
(2016): 7565–81. https://doi.org/10.1007/s00216-016-9800-9.'
chicago-de: 'Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard
K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis
und Christoph Gottschalk. 2016. Identification of Stachybotrys spp. by MALDI-TOF
mass spectrometry. Analytical & bioanalytical chemistry : a merger of
Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica
408, Nr. 27: 7565–7581. doi:10.1007/s00216-016-9800-9,
.'
din1505-2-1: 'Ulrich,
Sebastian ; Biermaier, Barbara
; Bader, Oliver ; Wolf,
Georg ; Straubinger, Reinhard K.
; Didier, Andrea ; Sperner,
Brigitte ; Schwaiger, Karin
; u. a.: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry.
In: Analytical & bioanalytical chemistry : a merger of Fresenius’ journal
of analytical chemistry, Analusis and Quimica analitica Bd. 408. Berlin ;
Heidelberg, Springer (2016), Nr. 27, S. 7565–7581'
havard: 'S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier,
B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Identification of Stachybotrys
spp. by MALDI-TOF mass spectrometry, Analytical & Bioanalytical Chemistry :
A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica.
408 (2016) 7565–7581.'
ieee: 'S. Ulrich et al., “Identification of Stachybotrys spp. by MALDI-TOF
mass spectrometry,” Analytical & bioanalytical chemistry : a merger of
Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica,
vol. 408, no. 27, pp. 7565–7581, 2016, doi: 10.1007/s00216-016-9800-9.'
mla: 'Ulrich, Sebastian, et al. “Identification of Stachybotrys Spp. by MALDI-TOF
Mass Spectrometry.” Analytical & Bioanalytical Chemistry : A Merger of
Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica,
vol. 408, no. 27, 2016, pp. 7565–81, https://doi.org/10.1007/s00216-016-9800-9.'
short: 'S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier,
B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Analytical & Bioanalytical
Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and
Quimica Analitica 408 (2016) 7565–7581.'
ufg: 'Ulrich, Sebastian u. a.: Identification of Stachybotrys spp. by MALDI-TOF
mass spectrometry, in: Analytical & bioanalytical chemistry : a merger
of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica
408 (2016), H. 27, S. 7565–7581.'
van: 'Ulrich S, Biermaier B, Bader O, Wolf G, Straubinger RK, Didier A, et al. Identification
of Stachybotrys spp. by MALDI-TOF mass spectrometry. Analytical & bioanalytical
chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and
Quimica analitica. 2016;408(27):7565–81.'
date_created: 2025-06-15T10:32:52Z
date_updated: 2025-06-18T12:11:46Z
department:
- _id: DEP4010
doi: 10.1007/s00216-016-9800-9
extern: '1'
intvolume: ' 408'
issue: '27'
keyword:
- Stachybotrys spp
- MALDI-TOF MS
- Mass spectrometry
- Filamentous fungi
language:
- iso: eng
page: 7565-7581
place: Berlin ; Heidelberg
publication: ' Analytical & bioanalytical chemistry : a merger of Fresenius'' journal
of analytical chemistry, Analusis and Quimica analitica'
publication_identifier:
eissn:
- 1618-2650
issn:
- 1618-2642
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry
type: scientific_journal_article
user_id: '83781'
volume: 408
year: '2016'
...
---
_id: '2357'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: 'Heiko '
full_name: 'Nerenz, Heiko '
last_name: Nerenz
- first_name: Jutta Maria
full_name: Quadflieg, Jutta Maria
last_name: Quadflieg
- first_name: Andreas
full_name: Schrader, Andreas
last_name: Schrader
- first_name: 'Verena '
full_name: 'Becker, Verena '
last_name: Becker
citation:
ama: 'Becker B, Nerenz H, Quadflieg JM, Schrader A, Becker V. Phtoalexine als antimikrobielle
Pflanzenwirkstoffe für die Kosmetik. SOFW-Journal : Home & personal care
ingredients & formulations; Deutsche Ausgabe. 2015;140(11):10-17.'
apa: 'Becker, B., Nerenz, H., Quadflieg, J. M., Schrader, A., & Becker, V. (2015).
Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik. SOFW-Journal :
Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe,
140(11), 10–17.'
bjps: 'Becker B et al. (2015) Phtoalexine Als Antimikrobielle Pflanzenwirkstoffe
Für Die Kosmetik. SOFW-Journal : Home & personal care ingredients &
formulations; Deutsche Ausgabe 140, 10–17.'
chicago: 'Becker, Barbara, Heiko Nerenz, Jutta Maria Quadflieg, Andreas Schrader,
and Verena Becker. “Phtoalexine Als Antimikrobielle Pflanzenwirkstoffe Für Die
Kosmetik.” SOFW-Journal : Home & Personal Care Ingredients & Formulations;
Deutsche Ausgabe 140, no. 11 (2015): 10–17.'
chicago-de: 'Becker, Barbara, Heiko Nerenz, Jutta Maria Quadflieg, Andreas Schrader
und Verena Becker. 2015. Phtoalexine als antimikrobielle Pflanzenwirkstoffe für
die Kosmetik. SOFW-Journal : Home & personal care ingredients & formulations;
Deutsche Ausgabe 140, Nr. 11: 10–17.'
din1505-2-1: 'Becker, Barbara ; Nerenz, Heiko ; Quadflieg,
Jutta Maria ; Schrader, Andreas
; Becker, Verena : Phtoalexine als
antimikrobielle Pflanzenwirkstoffe für die Kosmetik. In: SOFW-Journal : Home
& personal care ingredients & formulations; Deutsche Ausgabe Bd. 140,
Verl. für Chemische Industrie, Ziolkowsky (2015), Nr. 11, S. 10–17'
havard: 'B. Becker, H. Nerenz, J.M. Quadflieg, A. Schrader, V. Becker, Phtoalexine
als antimikrobielle Pflanzenwirkstoffe für die Kosmetik, SOFW-Journal : Home &
Personal Care Ingredients & Formulations; Deutsche Ausgabe. 140 (2015) 10–17.'
ieee: 'B. Becker, H. Nerenz, J. M. Quadflieg, A. Schrader, and V. Becker, “Phtoalexine
als antimikrobielle Pflanzenwirkstoffe für die Kosmetik,” SOFW-Journal : Home
& personal care ingredients & formulations; Deutsche Ausgabe, vol.
140, no. 11, pp. 10–17, 2015.'
mla: 'Becker, Barbara, et al. “Phtoalexine Als Antimikrobielle Pflanzenwirkstoffe
Für Die Kosmetik.” SOFW-Journal : Home & Personal Care Ingredients &
Formulations; Deutsche Ausgabe, vol. 140, no. 11, 2015, pp. 10–17.'
short: 'B. Becker, H. Nerenz, J.M. Quadflieg, A. Schrader, V. Becker, SOFW-Journal :
Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe 140
(2015) 10–17.'
ufg: 'Becker, Barbara u. a.: Phtoalexine als antimikrobielle Pflanzenwirkstoffe
für die Kosmetik, in: SOFW-Journal : Home & personal care ingredients &
formulations; Deutsche Ausgabe 140 (2015), H. 11, S. 10–17.'
van: 'Becker B, Nerenz H, Quadflieg JM, Schrader A, Becker V. Phtoalexine als antimikrobielle
Pflanzenwirkstoffe für die Kosmetik. SOFW-Journal : Home & personal care ingredients
& formulations; Deutsche Ausgabe. 2015;140(11):10–7.'
date_created: 2020-05-18T09:25:44Z
date_updated: 2024-12-02T14:22:03Z
ddc:
- '570'
- '572'
department:
- _id: DEP4010
file:
- access_level: closed
content_type: application/pdf
creator: z5h-0dr
date_created: 2020-05-18T09:25:39Z
date_updated: 2024-12-02T14:22:01Z
file_id: '2358'
file_name: BECKER(1).pdf
file_size: 2314359
relation: main_file
file_date_updated: 2024-12-02T14:22:01Z
has_accepted_license: '1'
intvolume: ' 140'
issue: '11'
language:
- iso: eng
page: 10-17
publication: 'SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe'
publication_identifier:
unknown:
- 0942-7694
publication_status: published
publisher: Verl. für Chemische Industrie, Ziolkowsky
status: public
title: Phtoalexine als antimikrobielle Pflanzenwirkstoffe für die Kosmetik
type: journal_article
user_id: '83781'
volume: 140
year: '2015'
...
---
_id: '12978'
abstract:
- lang: eng
text: Diet change and fatness are supposed to challenge the immune system of the
cow. Therefore, immunological and haematological consequences of adaptation to
and continued feeding of a high-energy diet were studied in eight non-pregnant,
non-lactating Holstein cows over 16 weeks. Blood haptoglobin concentration remained
unaltered, suggesting that an acute phase reaction was not induced. Stimulation
ability of peripheral blood mononuclear cells and stimulated oxidative burst capacity
of granulocytes increased significantly in the course of the experiment after
an initial drop. While total leucocyte counts increased, the proportion of granulocytes
increased and that of lymphocytes decreased at the same time as the ratio of CD4+/CD8+
lymphocytes did. Capability of rumen microbes to detoxify the immune-modulating
mycotoxin deoxynivalenol (DON) was not compromised as indicated by the exclusive
presence of de-DON as the detoxified DON metabolite in blood. In conclusion, both
diet change and prolonged positive energy balance influenced the bovine immune
system.
author:
- first_name: Sven
full_name: Dänicke, Sven
last_name: Dänicke
- first_name: Ulrich
full_name: Meyer, Ulrich
last_name: Meyer
- first_name: Janine
full_name: Winkler, Janine
last_name: Winkler
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Jana
full_name: Frahm, Jana
last_name: Frahm
- first_name: Susanne
full_name: Kersten, Susanne
last_name: Kersten
- first_name: Hana
full_name: Valenta, Hana
last_name: Valenta
- first_name: Jürgen
full_name: Rehage, Jürgen
last_name: Rehage
- first_name: Susanne
full_name: Häussler, Susanne
last_name: Häussler
- first_name: Helga
full_name: Sauerwein, Helga
last_name: Sauerwein
- first_name: Lena
full_name: Locher, Lena
last_name: Locher
citation:
ama: Dänicke S, Meyer U, Winkler J, et al. Haematological and immunological adaptations
of non-pregnant, non-lactating dairy cows to a high-energetic diet containing
mycotoxins. Archives of Animal Nutrition. 2015;70(1):1-16. doi:10.1080/1745039x.2015.1117561
apa: Dänicke, S., Meyer, U., Winkler, J., Ulrich, S., Frahm, J., Kersten, S., Valenta,
H., Rehage, J., Häussler, S., Sauerwein, H., & Locher, L. (2015). Haematological
and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic
diet containing mycotoxins. Archives of Animal Nutrition, 70(1),
1–16. https://doi.org/10.1080/1745039x.2015.1117561
bjps: Dänicke S et al. (2015) Haematological and Immunological Adaptations
of Non-Pregnant, Non-Lactating Dairy Cows to a High-Energetic Diet Containing
Mycotoxins. Archives of Animal Nutrition 70, 1–16.
chicago: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Sebastian Ulrich, Jana Frahm,
Susanne Kersten, Hana Valenta, et al. “Haematological and Immunological Adaptations
of Non-Pregnant, Non-Lactating Dairy Cows to a High-Energetic Diet Containing
Mycotoxins.” Archives of Animal Nutrition 70, no. 1 (2015): 1–16. https://doi.org/10.1080/1745039x.2015.1117561.'
chicago-de: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Sebastian Ulrich, Jana
Frahm, Susanne Kersten, Hana Valenta, u. a. 2015. Haematological and immunological
adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet
containing mycotoxins. Archives of Animal Nutrition 70, Nr. 1: 1–16. doi:10.1080/1745039x.2015.1117561,
.'
din1505-2-1: 'Dänicke,
Sven ; Meyer, Ulrich ; Winkler, Janine ; Ulrich,
Sebastian ; Frahm, Jana ;
Kersten, Susanne ; Valenta,
Hana ; Rehage, Jürgen ; u. a.:
Haematological and immunological adaptations of non-pregnant, non-lactating dairy
cows to a high-energetic diet containing mycotoxins. In: Archives of Animal
Nutrition Bd. 70. Abingdon, Taylor & Francis (2015), Nr. 1, S. 1–16'
havard: S. Dänicke, U. Meyer, J. Winkler, S. Ulrich, J. Frahm, S. Kersten, H. Valenta,
J. Rehage, S. Häussler, H. Sauerwein, L. Locher, Haematological and immunological
adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet
containing mycotoxins, Archives of Animal Nutrition. 70 (2015) 1–16.
ieee: 'S. Dänicke et al., “Haematological and immunological adaptations of
non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins,”
Archives of Animal Nutrition, vol. 70, no. 1, pp. 1–16, 2015, doi: 10.1080/1745039x.2015.1117561.'
mla: Dänicke, Sven, et al. “Haematological and Immunological Adaptations of Non-Pregnant,
Non-Lactating Dairy Cows to a High-Energetic Diet Containing Mycotoxins.” Archives
of Animal Nutrition, vol. 70, no. 1, 2015, pp. 1–16, https://doi.org/10.1080/1745039x.2015.1117561.
short: S. Dänicke, U. Meyer, J. Winkler, S. Ulrich, J. Frahm, S. Kersten, H. Valenta,
J. Rehage, S. Häussler, H. Sauerwein, L. Locher, Archives of Animal Nutrition
70 (2015) 1–16.
ufg: 'Dänicke, Sven u. a.: Haematological and immunological adaptations of
non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins,
in: Archives of Animal Nutrition 70 (2015), H. 1, S. 1–16.'
van: Dänicke S, Meyer U, Winkler J, Ulrich S, Frahm J, Kersten S, et al. Haematological
and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic
diet containing mycotoxins. Archives of Animal Nutrition. 2015;70(1):1–16.
date_created: 2025-06-15T10:34:57Z
date_updated: 2025-06-18T12:18:46Z
department:
- _id: DEP4010
doi: 10.1080/1745039x.2015.1117561
extern: '1'
intvolume: ' 70'
issue: '1'
keyword:
- Dairy cowsdeoxynivalenol
- energy consumption
- functional responses
- haematology
- leucocytes
- mycotoxins
- zearalenone
language:
- iso: eng
page: 1-16
place: Abingdon
publication: Archives of Animal Nutrition
publication_identifier:
eissn:
- 1477-2817
issn:
- 1745-039X
unknown:
- '0003-942X '
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
status: public
title: Haematological and immunological adaptations of non-pregnant, non-lactating
dairy cows to a high-energetic diet containing mycotoxins
type: scientific_journal_article
user_id: '83781'
volume: 70
year: '2015'
...
---
_id: '2363'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
citation:
ama: Becker B. useful for beverage spoilage microoganism. Brauwelt International.
2014:252-256.
apa: Becker, B. (2014). useful for beverage spoilage microoganism. Brauwelt International,
pp. 252–256.
bjps: Becker B (2014) Useful for Beverage Spoilage Microoganism. Brauwelt
International, 252–256.
chicago: Becker, Barbara. “Useful for Beverage Spoilage Microoganism.” Brauwelt
International, 2014.
chicago-de: Becker, Barbara. 2014. useful for beverage spoilage microoganism. Brauwelt
International.
din1505-2-1: 'Becker, Barbara: useful
for beverage spoilage microoganism. In: Brauwelt International (2014),
Nr. 5'
havard: B. Becker, useful for beverage spoilage microoganism, Brauwelt International.
(2014) 252–256.
ieee: B. Becker, “useful for beverage spoilage microoganism,” Brauwelt International,
no. 5, pp. 252–256, 2014.
mla: Becker, Barbara. “Useful for Beverage Spoilage Microoganism.” Brauwelt International,
no. 5, 2014, pp. 252–56.
short: B. Becker, Brauwelt International (2014) 252–256.
ufg: 'Becker, Barbara (2014): useful for beverage spoilage microoganism,
in: Brauwelt International (5) (2014), S. 252–256.'
van: Becker B. useful for beverage spoilage microoganism. Brauwelt International.
2014;252–6.
date_created: 2020-05-18T10:12:34Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4010
issue: '5'
language:
- iso: eng
page: 252-256
publication: Brauwelt International
publication_date: '2014'
publication_identifier:
unknown:
- 0934-9340
status: public
title: useful for beverage spoilage microoganism
type: newspaper_article
user_id: '74222'
year: 2014
...
---
_id: '2403'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Verena
full_name: Becker, Verena
last_name: Becker
- first_name: Heiko
full_name: Nerenz, Heiko
last_name: Nerenz
- first_name: Andreas
full_name: Schrader, Andreas
last_name: Schrader
citation:
ama: 'Becker B, Becker V, Nerenz H, Schrader A. Einsatz der Durchflusszytometrie
zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten.
SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe. 2014;140(5):26-27, 30.'
apa: 'Becker, B., Becker, V., Nerenz, H., & Schrader, A. (2014). Einsatz der
Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen
und Pflanzenextrakten. SOFW-Journal : Home & Personal Care Ingredients
& Formulations; Deutsche Ausgabe, 140(5), 26–27, 30.'
bjps: 'Becker B et al. (2014) Einsatz Der Durchflusszytometrie Zum
Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten.
SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe 140, 26–27, 30.'
chicago: 'Becker, Barbara, Verena Becker, Heiko Nerenz, and Andreas Schrader. “Einsatz
Der Durchflusszytometrie Zum Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen
Und Pflanzenextrakten.” SOFW-Journal : Home & Personal Care Ingredients
& Formulations; Deutsche Ausgabe 140, no. 5 (2014): 26–27, 30.'
chicago-de: 'Becker, Barbara, Verena Becker, Heiko Nerenz und Andreas Schrader.
2014. Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung
von Phytoalexinen und Pflanzenextrakten. SOFW-Journal : Home & personal
care ingredients & formulations; Deutsche Ausgabe 140, Nr. 5: 26–27, 30.'
din1505-2-1: 'Becker, Barbara ; Becker, Verena ; Nerenz,
Heiko ; Schrader, Andreas:
Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von
Phytoalexinen und Pflanzenextrakten. In: SOFW-Journal : Home & personal
care ingredients & formulations; Deutsche Ausgabe Bd. 140, Verl. für Chemische
Industrie, Ziolkowsky (2014), Nr. 5, S. 26–27, 30'
havard: 'B. Becker, V. Becker, H. Nerenz, A. Schrader, Einsatz der Durchflusszytometrie
zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten,
SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche
Ausgabe. 140 (2014) 26–27, 30.'
ieee: 'B. Becker, V. Becker, H. Nerenz, and A. Schrader, “Einsatz der Durchflusszytometrie
zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten,”
SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe, vol. 140, no. 5, pp. 26–27, 30, 2014.'
mla: 'Becker, Barbara, et al. “Einsatz Der Durchflusszytometrie Zum Nachweis Der
Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten.” SOFW-Journal :
Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe,
vol. 140, no. 5, 2014, pp. 26–27, 30.'
short: 'B. Becker, V. Becker, H. Nerenz, A. Schrader, SOFW-Journal : Home &
Personal Care Ingredients & Formulations; Deutsche Ausgabe 140 (2014) 26–27,
30.'
ufg: 'Becker, Barbara u. a.: Einsatz der Durchflusszytometrie zum Nachweis
der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten, in: SOFW-Journal :
Home & personal care ingredients & formulations; Deutsche Ausgabe
140 (2014), H. 5, S. 26–27, 30.'
van: 'Becker B, Becker V, Nerenz H, Schrader A. Einsatz der Durchflusszytometrie
zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten.
SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe. 2014;140(5):26–7, 30.'
date_created: 2020-05-20T07:28:39Z
date_updated: 2024-12-02T14:21:46Z
ddc:
- '610'
- '660'
department:
- _id: DEP4010
file:
- access_level: closed
content_type: application/pdf
creator: z5h-0dr
date_created: 2020-05-20T07:37:08Z
date_updated: 2024-12-02T14:21:45Z
file_id: '2405'
file_name: becker(2).pdf
file_size: 3196553
relation: main_file
file_date_updated: 2024-12-02T14:21:45Z
has_accepted_license: '1'
intvolume: ' 140'
issue: '5'
language:
- iso: eng
page: 26 - 27, 30
publication: 'SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe'
publication_identifier:
issn:
- 0942-7694
publication_status: published
publisher: Verl. für Chemische Industrie, Ziolkowsky
status: public
title: Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung
von Phytoalexinen und Pflanzenextrakten
type: journal_article
user_id: '83781'
volume: 140
year: '2014'
...
---
_id: '12979'
abstract:
- lang: eng
text: Physiological consequences of adaptation to and continued feeding of a high-energetic
diet were studied in eight non-pregnant, non-lactating dairy Holstein cows over
a period of 16 weeks. The first six weeks served as an adaptation period from
the low energetic straw-based diet (3.8 MJ NEL/kg DM) to the high-energetic ration
(7.5 MJ NEL/kg DM). Intake of dry matter (DM) increased with dietary energy concentration
from 9 to 20 kg/d up to week 9 to 12 and decreased thereafter. The initial live
weight (LW) of 550 ± 60 kg was increased linearly and corresponded to an average
daily LW gain of 2.3 ± 0.3 kg. Energy balance increased approximately nine-fold
to a maximum of 114 MJ NEL/d in week 10. Ruminal fermentation pattern was completely
changed from an acetate dominating profile to a propionate based one, which was
paralleled by a marked increase in the rumen fluid endotoxin concentration. Unlike
blood glucose concentration, which increased continuously, that of cholesterol
and triglycerides started to increase after an initial stagnation. In conclusion,
both ruminal adaptation to a high-energetic diet and the continued feeding of
such a diet induced digestive and metabolic adaptations in non-pregnant, non-lactating
cows characterised by a progressing positive energy balance.
author:
- first_name: Sven
full_name: Dänicke, Sven
last_name: Dänicke
- first_name: Ulrich
full_name: Meyer, Ulrich
last_name: Meyer
- first_name: Janine
full_name: Winkler, Janine
last_name: Winkler
- first_name: Kirsten
full_name: Schulz, Kirsten
last_name: Schulz
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Jana
full_name: Frahm, Jana
last_name: Frahm
- first_name: Susanne
full_name: Kersten, Susanne
last_name: Kersten
- first_name: Jürgen
full_name: Rehage, Jürgen
last_name: Rehage
- first_name: Gerhard
full_name: Breves, Gerhard
last_name: Breves
- first_name: Susanne
full_name: Häußler, Susanne
last_name: Häußler
- first_name: Helga
full_name: Sauerwein, Helga
last_name: Sauerwein
- first_name: Lena
full_name: Locher, Lena
last_name: Locher
citation:
ama: Dänicke S, Meyer U, Winkler J, et al. Description of a bovine model for studying
digestive and metabolic effects of a positive energy balance not biased by lactation
or gravidity. Archives of Animal Nutrition. 2014;68(6):460-477. doi:10.1080/1745039x.2014.973243
apa: Dänicke, S., Meyer, U., Winkler, J., Schulz, K., Ulrich, S., Frahm, J., Kersten,
S., Rehage, J., Breves, G., Häußler, S., Sauerwein, H., & Locher, L. (2014).
Description of a bovine model for studying digestive and metabolic effects of
a positive energy balance not biased by lactation or gravidity. Archives of
Animal Nutrition, 68(6), 460–477. https://doi.org/10.1080/1745039x.2014.973243
bjps: Dänicke S et al. (2014) Description of a Bovine Model for Studying
Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation
or Gravidity. Archives of Animal Nutrition 68, 460–477.
chicago: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian
Ulrich, Jana Frahm, Susanne Kersten, et al. “Description of a Bovine Model for
Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased
by Lactation or Gravidity.” Archives of Animal Nutrition 68, no. 6 (2014):
460–77. https://doi.org/10.1080/1745039x.2014.973243.'
chicago-de: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian
Ulrich, Jana Frahm, Susanne Kersten, u. a. 2014. Description of a bovine model
for studying digestive and metabolic effects of a positive energy balance not
biased by lactation or gravidity. Archives of Animal Nutrition 68, Nr.
6: 460–477. doi:10.1080/1745039x.2014.973243,
.'
din1505-2-1: 'Dänicke,
Sven ; Meyer, Ulrich ; Winkler, Janine ; Schulz,
Kirsten ; Ulrich, Sebastian
; Frahm, Jana ; Kersten,
Susanne ; Rehage, Jürgen
; u. a.: Description of a bovine model for studying digestive and metabolic
effects of a positive energy balance not biased by lactation or gravidity. In:
Archives of Animal Nutrition Bd. 68. Abingdon, Taylor & Francis (2014),
Nr. 6, S. 460–477'
havard: S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten,
J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Description of a bovine
model for studying digestive and metabolic effects of a positive energy balance
not biased by lactation or gravidity, Archives of Animal Nutrition. 68 (2014)
460–477.
ieee: 'S. Dänicke et al., “Description of a bovine model for studying digestive
and metabolic effects of a positive energy balance not biased by lactation or
gravidity,” Archives of Animal Nutrition, vol. 68, no. 6, pp. 460–477,
2014, doi: 10.1080/1745039x.2014.973243.'
mla: Dänicke, Sven, et al. “Description of a Bovine Model for Studying Digestive
and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or
Gravidity.” Archives of Animal Nutrition, vol. 68, no. 6, 2014, pp. 460–77,
https://doi.org/10.1080/1745039x.2014.973243.
short: S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten,
J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Archives of Animal
Nutrition 68 (2014) 460–477.
ufg: 'Dänicke, Sven u. a.: Description of a bovine model for studying digestive
and metabolic effects of a positive energy balance not biased by lactation or
gravidity, in: Archives of Animal Nutrition 68 (2014), H. 6, S. 460–477.'
van: Dänicke S, Meyer U, Winkler J, Schulz K, Ulrich S, Frahm J, et al. Description
of a bovine model for studying digestive and metabolic effects of a positive energy
balance not biased by lactation or gravidity. Archives of Animal Nutrition. 2014;68(6):460–77.
date_created: 2025-06-15T10:36:14Z
date_updated: 2025-06-18T12:23:01Z
department:
- _id: DEP4010
doi: 10.1080/1745039x.2014.973243
extern: '1'
intvolume: ' 68'
issue: '6'
keyword:
- blood chemistry
- dairy cows
- endotoxins
- energy balance
- energy content
- rumen fermentation
language:
- iso: eng
page: 460-477
place: Abingdon
publication: Archives of Animal Nutrition
publication_identifier:
eissn:
- 1477-2817
issn:
- 1745-039X
- 0003-942X
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
status: public
title: Description of a bovine model for studying digestive and metabolic effects
of a positive energy balance not biased by lactation or gravidity
type: scientific_journal_article
user_id: '83781'
volume: 68
year: '2014'
...
---
_id: '12984'
abstract:
- lang: eng
text: "Background\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder
characterized by late onset and progressive calcification of elastic fibers in
skin, eyes and the cardiovascular system, exemplifying a model for conditions
characterized by soft tissue calcification.\r\nObjective\r\nThe aim of our study
was to characterize cellular inorganic pyrophosphate (PPi) homeostasis in PXE.\r\nMethods\r\nGene
expression of PPi metabolizing enzymes was determined by quantitative real-time
PCR after incubation up to 21 days with or without addition of Na2HPO4. Extracellular
and cytosolic PPi concentrations were measured by enzyme-linked bioluminescence
assay. ALP and ENPP1 activity was determined spectrophotometrically. We further
established a human cell culture model suitable for investigating PXE and related
disorders without addition of artificial calcification triggers.\r\nResults\r\nIndependently
of the experimental conditions, PXE fibroblasts revealed a higher degree of matrix
calcification. We observed that matrix calcification was associated with altered
gene expression of PPi metabolizing enzymes in PXE fibroblasts. In this context,
PXE fibroblasts exhibited significantly higher expression of ALP and OPN and reduced
mRNA expression and activity of ENPP1. Here, for the first time cytosolic and
extracellular PPi levels were shown to be strongly reduced in PXE fibroblasts.
We further showed that PPi concentration in bovine and human sera additives had
a strong impact on matrix calcification. In a last experimental line, we demonstrated
that addition of PPi analogs reduced matrix calcification of PXE fibroblasts most
likely by reducing ALP and OPN mRNA expression, restoring ENPP1 activity and subsequently
elevating PPi concentrations.\r\nConclusion\r\nThe results of our study along
with recent findings point to the essential role of PPi as the central regulatory
metabolites preventing matrix calcification in PXE. But what remains to be determined
is the underlying molecular mechanism leading to depletion of PPi in PXE. We further
suggest that supplementation of PPi analogs might counteract pathological calcification
in PXE and related disorders."
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Patricia
full_name: Kuzaj, Patricia
last_name: Kuzaj
- first_name: Christian
full_name: Götting, Christian
last_name: Götting
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Doris
full_name: Hendig, Doris
last_name: Hendig
citation:
ama: Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as
a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal
of Dermatological Science. 2014;75(2):109-120. doi:10.1016/j.jdermsci.2014.04.015
apa: Dabisch-Ruthe, M., Kuzaj, P., Götting, C., Knabbe, C., & Hendig, D. (2014).
Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma
elasticum. Journal of Dermatological Science, 75(2), 109–120. https://doi.org/10.1016/j.jdermsci.2014.04.015
bjps: Dabisch-Ruthe M et al. (2014) Pyrophosphates as a Major Inhibitor
of Matrix Calcification in Pseudoxanthoma Elasticum. Journal of Dermatological
Science 75, 109–120.
chicago: 'Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius Knabbe,
and Doris Hendig. “Pyrophosphates as a Major Inhibitor of Matrix Calcification
in Pseudoxanthoma Elasticum.” Journal of Dermatological Science 75, no.
2 (2014): 109–20. https://doi.org/10.1016/j.jdermsci.2014.04.015.'
chicago-de: 'Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius
Knabbe und Doris Hendig. 2014. Pyrophosphates as a major inhibitor of matrix calcification
in Pseudoxanthoma elasticum. Journal of Dermatological Science 75, Nr.
2: 109–120. doi:10.1016/j.jdermsci.2014.04.015,
.'
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Kuzaj, Patricia ; Götting,
Christian ; Knabbe, Cornelius
; Hendig, Doris: Pyrophosphates
as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. In:
Journal of Dermatological Science Bd. 75. Amsterdam, Elsevier (2014),
Nr. 2, S. 109–120'
havard: M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Pyrophosphates
as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum, Journal
of Dermatological Science. 75 (2014) 109–120.
ieee: 'M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, and D. Hendig, “Pyrophosphates
as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum,” Journal
of Dermatological Science, vol. 75, no. 2, pp. 109–120, 2014, doi: 10.1016/j.jdermsci.2014.04.015.'
mla: Dabisch-Ruthe, Mareike, et al. “Pyrophosphates as a Major Inhibitor of Matrix
Calcification in Pseudoxanthoma Elasticum.” Journal of Dermatological Science,
vol. 75, no. 2, 2014, pp. 109–20, https://doi.org/10.1016/j.jdermsci.2014.04.015.
short: M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Journal of
Dermatological Science 75 (2014) 109–120.
ufg: 'Dabisch-Ruthe, Mareike u. a.: Pyrophosphates as a major inhibitor of
matrix calcification in Pseudoxanthoma elasticum, in: Journal of Dermatological
Science 75 (2014), H. 2, S. 109–120.'
van: Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as
a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal
of Dermatological Science. 2014;75(2):109–20.
date_created: 2025-06-17T06:52:59Z
date_updated: 2025-06-17T13:48:41Z
department:
- _id: DEP4010
doi: 10.1016/j.jdermsci.2014.04.015
intvolume: ' 75'
issue: '2'
keyword:
- Pseudoxanthoma elasticum
- Calcification
- Pyrophosphate
- Bisphosphonate
- ABCC6
- Tissue nonspecific alkaline phosphate
- Ectonucleotide pyrophosphatase 1
language:
- iso: eng
page: 109-120
place: Amsterdam
publication: Journal of Dermatological Science
publication_identifier:
eissn:
- 1873-569X
issn:
- 0923-1811
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma
elasticum
type: scientific_journal_article
user_id: '83781'
volume: 75
year: '2014'
...
---
_id: '12985'
abstract:
- lang: eng
text: "Objectives\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder
characterized by progressive calcification and fragmentation of elastic fibers.
Because of the great clinical variability between PXE patients the involvement
of modifier genes was recently suggested. Therefore, we investigated the association
of single nucleotide variants (SNVs) in selected candidate genes known to regulate
cellular pyrophosphate metabolism.\r\nDesign and methods\r\nWe used RLFP analyses
to evaluate the distribution of SNVs in alkaline phosphatase (ALP), ectonucleotide
pyrophosphatase 1 (ENPP1) and ankylosis (ANKH) in DNA samples from 190 German
PXE patients and 190 age- and sex-matched healthy controls. Statistical analyses
were performed using Fisher exact test and Bonferroni correction.\r\nResults\r\nThe
screening revealed three different SNVs in three genes, which were associated
with PXE. The SNV c.1190-65C > A (rs1780329, minor allele frequency (MAF) patients:
0.17; controls: 0.11; P = 0.04) in the ALP gene was significantly more frequent
in PXE patients. Furthermore, PXE was highly associated with ANKH p.A98A genotype
TT (P = 0.0012), although the MAF was not different between patients and controls.
After correction for multiple testing according to the Bonferroni method, one
SNV in the ENPP1 gene (c.313 + 9G > T, rs7773477) remained significantly associated
with PXE with significantly higher MAF values in the patient cohort (MAF: 0.04
vs. 0.00; P = 0.0024) and a high association with PXE susceptibility (OR 27.96).\r\nConclusion\r\nPolymorphisms
in ALP, ENPP1 and ANKH are important genetic risk factors contributing to PXE."
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Alexander
full_name: Brock, Alexander
last_name: Brock
- first_name: Patricia
full_name: Kuzaj, Patricia
last_name: Kuzaj
- first_name: Peter
full_name: Charbel Issa, Peter
last_name: Charbel Issa
- first_name: Christiane
full_name: Szliska, Christiane
last_name: Szliska
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Doris
full_name: Hendig, Doris
last_name: Hendig
citation:
ama: Dabisch-Ruthe M, Brock A, Kuzaj P, et al. Variants in genes encoding pyrophosphate
metabolizing enzymes are associated with Pseudoxanthoma elasticum. Clinical
Biochemistry. 2014;47(15):60-67. doi:10.1016/j.clinbiochem.2014.07.003
apa: Dabisch-Ruthe, M., Brock, A., Kuzaj, P., Charbel Issa, P., Szliska, C., Knabbe,
C., & Hendig, D. (2014). Variants in genes encoding pyrophosphate metabolizing
enzymes are associated with Pseudoxanthoma elasticum. Clinical Biochemistry,
47(15), 60–67. https://doi.org/10.1016/j.clinbiochem.2014.07.003
bjps: Dabisch-Ruthe M et al. (2014) Variants in Genes Encoding Pyrophosphate
Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum. Clinical
Biochemistry 47, 60–67.
chicago: 'Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel
Issa, Christiane Szliska, Cornelius Knabbe, and Doris Hendig. “Variants in Genes
Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma
Elasticum.” Clinical Biochemistry 47, no. 15 (2014): 60–67. https://doi.org/10.1016/j.clinbiochem.2014.07.003.'
chicago-de: 'Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel
Issa, Christiane Szliska, Cornelius Knabbe und Doris Hendig. 2014. Variants in
genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma
elasticum. Clinical Biochemistry 47, Nr. 15: 60–67. doi:10.1016/j.clinbiochem.2014.07.003,
.'
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Brock, Alexander ; Kuzaj,
Patricia ; Charbel Issa, Peter
; Szliska, Christiane ; Knabbe,
Cornelius ; Hendig, Doris:
Variants in genes encoding pyrophosphate metabolizing enzymes are associated with
Pseudoxanthoma elasticum. In: Clinical Biochemistry Bd. 47. Amsterdam,
Elsevier (2014), Nr. 15, S. 60–67'
havard: M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe,
D. Hendig, Variants in genes encoding pyrophosphate metabolizing enzymes are associated
with Pseudoxanthoma elasticum, Clinical Biochemistry. 47 (2014) 60–67.
ieee: 'M. Dabisch-Ruthe et al., “Variants in genes encoding pyrophosphate
metabolizing enzymes are associated with Pseudoxanthoma elasticum,” Clinical
Biochemistry, vol. 47, no. 15, pp. 60–67, 2014, doi: 10.1016/j.clinbiochem.2014.07.003.'
mla: Dabisch-Ruthe, Mareike, et al. “Variants in Genes Encoding Pyrophosphate Metabolizing
Enzymes Are Associated with Pseudoxanthoma Elasticum.” Clinical Biochemistry,
vol. 47, no. 15, 2014, pp. 60–67, https://doi.org/10.1016/j.clinbiochem.2014.07.003.
short: M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe,
D. Hendig, Clinical Biochemistry 47 (2014) 60–67.
ufg: 'Dabisch-Ruthe, Mareike u. a.: Variants in genes encoding pyrophosphate
metabolizing enzymes are associated with Pseudoxanthoma elasticum, in: Clinical
Biochemistry 47 (2014), H. 15, S. 60–67.'
van: Dabisch-Ruthe M, Brock A, Kuzaj P, Charbel Issa P, Szliska C, Knabbe C, et
al. Variants in genes encoding pyrophosphate metabolizing enzymes are associated
with Pseudoxanthoma elasticum. Clinical Biochemistry. 2014;47(15):60–7.
date_created: 2025-06-17T06:53:30Z
date_updated: 2025-06-17T13:48:52Z
department:
- _id: DEP4010
doi: 10.1016/j.clinbiochem.2014.07.003
intvolume: ' 47'
issue: '15'
keyword:
- Pseudoxanthoma elasticum
- Calcification
- Tissue nonspecific alkaline phosphatase
- Ectonucleotide pyrophosphatase 1
- Ankylosis
language:
- iso: eng
page: 60-67
place: Amsterdam
publication: Clinical Biochemistry
publication_identifier:
eissn:
- 1873-2933
issn:
- 0009-9120
publication_status: published
publisher: Elsevier
status: public
title: Variants in genes encoding pyrophosphate metabolizing enzymes are associated
with Pseudoxanthoma elasticum
type: scientific_journal_article
user_id: '83781'
volume: 47
year: '2014'
...
---
_id: '12986'
abstract:
- lang: eng
text: "Background\r\nDysregulations in cholesterol and lipid metabolism have been
linked to human diseases like hypercholesterolemia, atherosclerosis or the metabolic
syndrome. Many ABC transporters are involved in trafficking of metabolites derived
from these pathways. Pseudoxanthoma elasticum (PXE), an autosomal-recessive disease
caused by ABCC6 mutations, is characterized by atherogenesis and soft tissue calcification.\r\nMethods\r\nIn
this study we investigated the regulation of cholesterol biosynthesis in human
dermal fibroblasts from PXE patients and healthy controls.\r\nResults\r\nGene
expression analysis of 84 targets indicated dysregulations in cholesterol metabolism
in PXE fibroblasts. Transcript levels of ABCC6 were strongly increased in lipoprotein-deficient
serum (LPDS) and under serum starvation in healthy controls. For the first time,
increased HMG CoA reductase activities were found in PXE fibroblasts. We further
observed strongly elevated transcript and protein levels for the proprotein convertase
subtilisin/kexin type 9 (PCSK9), as well as a significant reduction in APOE mRNA
expression in PXE.\r\nConclusion\r\nIncreased cholesterol biosynthesis, elevated
PCSK9 levels and reduced APOE mRNA expression newly found in PXE fibroblasts could
enforce atherogenesis and cardiovascular risk in PXE patients. Moreover, the increase
in ABCC6 expression accompanied by the induction of cholesterol biosynthesis supposes
a functional role for ABCC6 in human lipoprotein and cholesterol homeostasis."
article_number: '118'
author:
- first_name: Patricia
full_name: Kuzaj, Patricia
last_name: Kuzaj
- first_name: Joachim
full_name: Kuhn, Joachim
last_name: Kuhn
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Isabel
full_name: Faust, Isabel
last_name: Faust
- first_name: Christian
full_name: Götting, Christian
last_name: Götting
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Doris
full_name: Hendig, Doris
last_name: Hendig
citation:
ama: Kuzaj P, Kuhn J, Dabisch-Ruthe M, et al. ABCC6- a new player in cellular cholesterol
and lipoprotein metabolism? Lipids in Health and Disease. 2014;13(1). doi:10.1186/1476-511x-13-118
apa: Kuzaj, P., Kuhn, J., Dabisch-Ruthe, M., Faust, I., Götting, C., Knabbe, C.,
& Hendig, D. (2014). ABCC6- a new player in cellular cholesterol and lipoprotein
metabolism? Lipids in Health and Disease, 13(1), Article 118. https://doi.org/10.1186/1476-511x-13-118
bjps: Kuzaj P et al. (2014) ABCC6- a New Player in Cellular Cholesterol
and Lipoprotein Metabolism? Lipids in Health and Disease 13.
chicago: Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust, Christian
Götting, Cornelius Knabbe, and Doris Hendig. “ABCC6- a New Player in Cellular
Cholesterol and Lipoprotein Metabolism?” Lipids in Health and Disease 13,
no. 1 (2014). https://doi.org/10.1186/1476-511x-13-118.
chicago-de: Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust,
Christian Götting, Cornelius Knabbe und Doris Hendig. 2014. ABCC6- a new player
in cellular cholesterol and lipoprotein metabolism? Lipids in Health and Disease
13, Nr. 1. doi:10.1186/1476-511x-13-118,
.
din1505-2-1: 'Kuzaj, Patricia ; Kuhn, Joachim ; Dabisch-Ruthe,
Mareike ; Faust, Isabel ;
Götting, Christian ; Knabbe,
Cornelius ; Hendig, Doris:
ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? In: Lipids
in Health and Disease Bd. 13. London, Biomed Central (2014), Nr. 1'
havard: P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D.
Hendig, ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?,
Lipids in Health and Disease. 13 (2014).
ieee: 'P. Kuzaj et al., “ABCC6- a new player in cellular cholesterol and
lipoprotein metabolism?,” Lipids in Health and Disease, vol. 13, no. 1,
Art. no. 118, 2014, doi: 10.1186/1476-511x-13-118.'
mla: Kuzaj, Patricia, et al. “ABCC6- a New Player in Cellular Cholesterol and Lipoprotein
Metabolism?” Lipids in Health and Disease, vol. 13, no. 1, 118, 2014, https://doi.org/10.1186/1476-511x-13-118.
short: P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D.
Hendig, Lipids in Health and Disease 13 (2014).
ufg: 'Kuzaj, Patricia u. a.: ABCC6- a new player in cellular cholesterol
and lipoprotein metabolism?, in: Lipids in Health and Disease 13 (2014),
H. 1.'
van: Kuzaj P, Kuhn J, Dabisch-Ruthe M, Faust I, Götting C, Knabbe C, et al. ABCC6-
a new player in cellular cholesterol and lipoprotein metabolism? Lipids in Health
and Disease. 2014;13(1).
date_created: 2025-06-17T06:53:58Z
date_updated: 2025-06-17T13:49:15Z
department:
- _id: DEP4010
doi: 10.1186/1476-511x-13-118
intvolume: ' 13'
issue: '1'
keyword:
- Pseudoxanthoma elasticum
- ABC transporter
- ABCC6
- Cholesterol biosynthesis
- Atherosclerosis
- HMG CoA reductase
- SREBP2
- PCSK9
- LDLR
- APOE
language:
- iso: eng
place: London
publication: Lipids in Health and Disease
publication_identifier:
eissn:
- 1476-511X
publication_status: published
publisher: 'Biomed Central '
quality_controlled: '1'
status: public
title: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?
type: scientific_journal_article
user_id: '83781'
volume: 13
year: '2014'
...
---
_id: '12987'
abstract:
- lang: eng
text: Mutations in the ABC transporter ABCC6 were recently identified as cause of
Pseudoxanthoma elasticum (PXE), a rare genetic disorder characterized by progressive
mineralization of elastic fibers. We used an untargeted metabolic approach to
identify biochemical differences between human dermal fibroblasts from healthy
controls and PXE patients in an attempt to find a link between ABCC6 deficiency,
cellular metabolic alterations and disease pathogenesis. 358 compounds were identified
by mass spectrometry covering lipids, amino acids, peptides, carbohydrates, nucleotides,
vitamins and cofactors, xenobiotics and energy metabolites. We found substantial
differences in glycerophospholipid composition, leucine dipeptides, and polypeptides
as well as alterations in pantothenate and guanine metabolism to be significantly
associated with PXE pathogenesis. These findings can be linked to extracellular
matrix remodeling and increased oxidative stress, which reflect characteristic
hallmarks of PXE. Our study could facilitate a better understanding of biochemical
pathways involved in soft tissue mineralization.
article_number: e108336
author:
- first_name: Patricia
full_name: Kuzaj, Patricia
last_name: Kuzaj
- first_name: Joachim
full_name: Kuhn, Joachim
last_name: Kuhn
- first_name: Ryan D.
full_name: Michalek, Ryan D.
last_name: Michalek
- first_name: Edward D.
full_name: Karoly, Edward D.
last_name: Karoly
- first_name: Isabel
full_name: Faust, Isabel
last_name: Faust
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Doris
full_name: Hendig, Doris
last_name: Hendig
citation:
ama: Kuzaj P, Kuhn J, Michalek RD, et al. Large-Scaled Metabolic Profiling of Human
Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
Controls. PLOS ONE / Public Library of Science . 2014;9(9). doi:10.1371/journal.pone.0108336
apa: Kuzaj, P., Kuhn, J., Michalek, R. D., Karoly, E. D., Faust, I., Dabisch-Ruthe,
M., Knabbe, C., & Hendig, D. (2014). Large-Scaled Metabolic Profiling of Human
Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
Controls. PLOS ONE / Public Library of Science , 9(9), Article e108336.
https://doi.org/10.1371/journal.pone.0108336
bjps: Kuzaj P et al. (2014) Large-Scaled Metabolic Profiling of Human
Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
Controls. PLOS ONE / Public Library of Science 9.
chicago: Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel
Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe, and Doris Hendig. “Large-Scaled
Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
Patients and Healthy Controls.” PLOS ONE / Public Library of Science 9,
no. 9 (2014). https://doi.org/10.1371/journal.pone.0108336.
chicago-de: Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel
Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe und Doris Hendig. 2014. Large-Scaled
Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
Patients and Healthy Controls. PLOS ONE / Public Library of Science 9,
Nr. 9. doi:10.1371/journal.pone.0108336,
.
din1505-2-1: 'Kuzaj, Patricia ; Kuhn, Joachim ; Michalek,
Ryan D. ; Karoly, Edward D.
; Faust, Isabel ; Dabisch-Ruthe,
Mareike ; Knabbe, Cornelius
; Hendig, Doris: Large-Scaled Metabolic
Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients
and Healthy Controls. In: PLOS ONE / Public Library of Science Bd. 9.
San Francisco, California, US, Public Library of Science (PLoS) (2014), Nr. 9'
havard: P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe,
C. Knabbe, D. Hendig, Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts
Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls, PLOS ONE
/ Public Library of Science . 9 (2014).
ieee: 'P. Kuzaj et al., “Large-Scaled Metabolic Profiling of Human Dermal
Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls,”
PLOS ONE / Public Library of Science , vol. 9, no. 9, Art. no. e108336,
2014, doi: 10.1371/journal.pone.0108336.'
mla: Kuzaj, Patricia, et al. “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts
Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls.” PLOS
ONE / Public Library of Science , vol. 9, no. 9, e108336, 2014, https://doi.org/10.1371/journal.pone.0108336.
short: P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe,
C. Knabbe, D. Hendig, PLOS ONE / Public Library of Science 9 (2014).
ufg: 'Kuzaj, Patricia u. a.: Large-Scaled Metabolic Profiling of Human Dermal
Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls,
in: PLOS ONE / Public Library of Science 9 (2014), H. 9.'
van: Kuzaj P, Kuhn J, Michalek RD, Karoly ED, Faust I, Dabisch-Ruthe M, et al. Large-Scaled
Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
Patients and Healthy Controls. PLOS ONE / Public Library of Science . 2014;9(9).
date_created: 2025-06-17T06:54:30Z
date_updated: 2025-06-17T13:49:26Z
department:
- _id: DEP4010
doi: 10.1371/journal.pone.0108336
intvolume: ' 9'
issue: '9'
language:
- iso: eng
place: San Francisco, California, US
publication: 'PLOS ONE / Public Library of Science '
publication_identifier:
eissn:
- 1932-6203
publication_status: published
publisher: Public Library of Science (PLoS)
quality_controlled: '1'
status: public
title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma
Elasticum Patients and Healthy Controls
type: scientific_journal_article
user_id: '83781'
volume: 9
year: '2014'
...
---
_id: '5348'
author:
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
citation:
ama: 'Schwarzer K, Schneider J, Becker B, Müller U. Entkeimungsauslegung mit der
"Lemgo D- and z-value Database for Food. In: ; 2012.'
apa: Schwarzer, K., Schneider, J., Becker, B., & Müller, U. (2012). Entkeimungsauslegung
mit der "Lemgo D- and z-value Database for Food. ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik
, Stuttgart - Hohenheim.
bjps: Schwarzer K et al. (2012) Entkeimungsauslegung mit der "Lemgo
D- and z-value Database for Food.
chicago: Schwarzer, Knut, Jan Schneider, Barbara Becker, and Ulrich Müller. “Entkeimungsauslegung
mit der "Lemgo D- and z-value Database for Food,” 2012.
chicago-de: 'Schwarzer, Knut, Jan Schneider, Barbara Becker und Ulrich Müller. 2012.
Entkeimungsauslegung mit der "Lemgo D- and z-value Database for Food. In: .'
din1505-2-1: 'Schwarzer, Knut ; Schneider, Jan ; Becker,
Barbara ; Müller, Ulrich:
Entkeimungsauslegung mit der "Lemgo D- and z-value Database for Food. In: , 2012'
havard: 'K. Schwarzer, J. Schneider, B. Becker, U. Müller, Entkeimungsauslegung
mit der "Lemgo D- and z-value Database for Food, in: 2012.'
ieee: K. Schwarzer, J. Schneider, B. Becker, and U. Müller, “Entkeimungsauslegung
mit der "Lemgo D- and z-value Database for Food,” presented at the ProcessNet-Fachgruppe
Lebensmittelverfahrenstechnik , Stuttgart - Hohenheim, 2012.
mla: Schwarzer, Knut, et al. Entkeimungsauslegung mit der "Lemgo D- and z-value
Database for Food. 2012.
short: 'K. Schwarzer, J. Schneider, B. Becker, U. Müller, in: 2012.'
ufg: 'Schwarzer, Knut u. a.: Entkeimungsauslegung mit der "Lemgo D- and z-value
Database for Food, in: o. Hg.: o. O. 2012.'
van: Schwarzer K, Schneider J, Becker B, Müller U. Entkeimungsauslegung mit der
"Lemgo D- and z-value Database for Food. In 2012.
conference:
end_date: 2012-03-21
location: Stuttgart - Hohenheim
name: 'ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik '
start_date: 2012-03-19
date_created: 2021-03-30T07:24:31Z
date_updated: 2024-05-24T06:46:24Z
department:
- _id: DEP4009
- _id: DEP4023
- _id: DEP4010
- _id: DEP4018
language:
- iso: ger
status: public
title: Entkeimungsauslegung mit der "Lemgo D- and z-value Database for Food
type: conference
user_id: '83779'
year: '2012'
...
---
_id: '12983'
abstract:
- lang: eng
text: "Background\r\nA broad spectrum of pathogens is causative for respiratory
tract infections, but symptoms are mostly similar. Therefore, the identification
of the causative viruses and bacteria is only feasible using multiplex PCR or
several monoplex PCR tests in parallel.\r\nMethods\r\nThe analytical sensitivity
of three multiplex PCR assays, RespiFinder-19, RespiFinder-SMART-22 and xTAG-Respiratory-Virus-Panel-Fast-Assay
(RVP), were compared to monoplex real-time PCR with quantified standardized control
material. All assays include the most common respiratory pathogens.\r\nResults\r\nTo
compare the analytical sensitivity of the multiplex assays, samples were inoculated
with 13 different quantified viruses in the range of 101 to 105 copies/ml. Concordant
results were received for rhinovirus, whereas the RVP detected influenzavirus,
RSV and hMPV more frequently in low concentrations. The RespiFinder-19 and the
RespiFinder-SMART-22 showed a higher analytical sensitivity for adenoviruses and
coronaviruses, whereas the RVP was incapable to detect adenovirus and coronavirus
in concentrations of 104 copies/ml. The RespiFinder-19 and RespiFinder-SMART-22A
did not detect influenzaviruses (104 copies/ml) and RSV (103 copies/ml). The detection
of all 13 viruses in one sample was only achieved using monoplex PCR. To analyze
possible competitive amplification reactions between the different viruses, samples
were further inoculated with only 4 different viruses in one sample. Compared
to the detection of 13 viruses in parallel, only a few differences were found.\r\nThe
incidence of respiratory viruses was compared in tracheal secretion (TS) samples
(n = 100) of mechanically ventilated patients in winter (n = 50) and summer (n = 50).
In winter, respiratory viruses were detected in 32 TS samples (64%) by RespiFinder-19,
whereas the detection rate with RVP was only 22%. The most frequent viruses were
adenovirus (32%) and PIV-2 (20%). Multiple infections were detected in 16 TS samples
(32%) by RespiFinder-19. Fewer infections were found in summer (RespiFinder-19:
20%; RVP: 6%). All positive results were verified using monoplex PCR.\r\nConclusions\r\nMultiplex
PCR tests have a broad spectrum of pathogens to test at a time. Analysis of multiple
inoculated samples revealed a different focus of the detected virus types by the
three assays. Analysis of clinical samples showed a high concordance of detected
viruses by the RespiFinder-19 compared to monoplex tests."
article_number: '163'
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Tanja
full_name: Vollmer, Tanja
last_name: Vollmer
- first_name: Ortwin
full_name: Adams, Ortwin
last_name: Adams
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Jens
full_name: Dreier, Jens
last_name: Dreier
citation:
ama: 'Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. BMC Infectious Diseases. 2012;12(1). doi:10.1186/1471-2334-12-163'
apa: 'Dabisch-Ruthe, M., Vollmer, T., Adams, O., Knabbe, C., & Dreier, J. (2012).
Comparison of three multiplex PCR assays for the detection of respiratory viral
infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder
19 assay and RespiFinder SMART 22 assay. BMC Infectious Diseases, 12(1),
Article 163. https://doi.org/10.1186/1471-2334-12-163'
bjps: 'Dabisch-Ruthe M et al. (2012) Comparison of Three Multiplex
PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG
Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART
22 Assay. BMC Infectious Diseases 12.'
chicago: 'Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe,
and Jens Dreier. “Comparison of Three Multiplex PCR Assays for the Detection of
Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast
Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” BMC Infectious
Diseases 12, no. 1 (2012). https://doi.org/10.1186/1471-2334-12-163.'
chicago-de: 'Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe
und Jens Dreier. 2012. Comparison of three multiplex PCR assays for the detection
of respiratory viral infections: evaluation of xTAG respiratory virus panel fast
assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infectious
Diseases 12, Nr. 1. doi:10.1186/1471-2334-12-163,
.'
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Vollmer, Tanja ; Adams,
Ortwin ; Knabbe, Cornelius
; Dreier, Jens: Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. In: BMC Infectious Diseases Bd. 12. Berlin ; Heidelberg,
Springer (2012), Nr. 1'
havard: 'M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, Comparison
of three multiplex PCR assays for the detection of respiratory viral infections:
evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and
RespiFinder SMART 22 assay, BMC Infectious Diseases. 12 (2012).'
ieee: 'M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, and J. Dreier, “Comparison
of three multiplex PCR assays for the detection of respiratory viral infections:
evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and
RespiFinder SMART 22 assay,” BMC Infectious Diseases, vol. 12, no. 1, Art.
no. 163, 2012, doi: 10.1186/1471-2334-12-163.'
mla: 'Dabisch-Ruthe, Mareike, et al. “Comparison of Three Multiplex PCR Assays for
the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory
Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.”
BMC Infectious Diseases, vol. 12, no. 1, 163, 2012, https://doi.org/10.1186/1471-2334-12-163.'
short: M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, BMC Infectious
Diseases 12 (2012).
ufg: 'Dabisch-Ruthe, Mareike u. a.: Comparison of three multiplex PCR assays
for the detection of respiratory viral infections: evaluation of xTAG respiratory
virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, in:
BMC Infectious Diseases 12 (2012), H. 1.'
van: 'Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. BMC Infectious Diseases. 2012;12(1).'
date_created: 2025-06-17T06:52:13Z
date_updated: 2025-06-17T13:49:03Z
department:
- _id: DEP4010
doi: 10.1186/1471-2334-12-163
intvolume: ' 12'
issue: '1'
keyword:
- Respiratory Virus
- Elution Volume
- Multiplex Assay
- Multiple Infection
- Bocavirus
language:
- iso: eng
place: Berlin ; Heidelberg
publication: BMC Infectious Diseases
publication_identifier:
eissn:
- 1471-2334
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: 'Comparison of three multiplex PCR assays for the detection of respiratory
viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder
19 assay and RespiFinder SMART 22 assay'
type: scientific_journal_article
user_id: '83781'
volume: 12
year: '2012'
...
---
_id: '2748'
author:
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Patrick
full_name: Wilhelm, Patrick
id: '12013'
last_name: Wilhelm
citation:
ama: 'Schneider J, Müller U, Schwarzer K, Becker B, Wilhelm P. Lemgo Database for
D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International.
2010;(5):252-256.'
apa: 'Schneider, J., Müller, U., Schwarzer, K., Becker, B., & Wilhelm, P. (2010).
Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim.
Brauwelt International, 5, 252–256.'
bjps: 'Schneider J et al. (2010) Lemgo Database for D- and z-Values:
Useful for Beverage Spoilage Microorgansim. Brauwelt International 252–256.'
chicago: 'Schneider, Jan, Ulrich Müller, Knut Schwarzer, Barbara Becker, and Patrick
Wilhelm. “Lemgo Database for D- and z-Values: Useful for Beverage Spoilage Microorgansim.”
Brauwelt International, no. 5 (2010): 252–56.'
chicago-de: 'Schneider, Jan, Ulrich Müller, Knut Schwarzer, Barbara Becker und Patrick
Wilhelm. 2010. Lemgo Database for D- and z-values: useful for beverage spoilage
microorgansim. Brauwelt International, Nr. 5: 252–256.'
din1505-2-1: 'Schneider, Jan ; Müller, Ulrich ; Schwarzer,
Knut ; Becker, Barbara ;
Wilhelm, Patrick: Lemgo Database
for D- and z-values: useful for beverage spoilage microorgansim. In: Brauwelt
International, Fachverlag Hans Carl GmbH (2010), Nr. 5, S. 252–256'
havard: 'J. Schneider, U. Müller, K. Schwarzer, B. Becker, P. Wilhelm, Lemgo Database
for D- and z-values: useful for beverage spoilage microorgansim, Brauwelt International.
(2010) 252–256.'
ieee: 'J. Schneider, U. Müller, K. Schwarzer, B. Becker, and P. Wilhelm, “Lemgo
Database for D- and z-values: useful for beverage spoilage microorgansim,” Brauwelt
International, no. 5, pp. 252–256, 2010.'
mla: 'Schneider, Jan, et al. “Lemgo Database for D- and z-Values: Useful for Beverage
Spoilage Microorgansim.” Brauwelt International, no. 5, 2010, pp. 252–56.'
short: J. Schneider, U. Müller, K. Schwarzer, B. Becker, P. Wilhelm, Brauwelt International
(2010) 252–256.
ufg: 'Schneider, Jan u. a.: Lemgo Database for D- and z-values: useful for
beverage spoilage microorgansim, in: Brauwelt International (2010), H.
5, S. 252–256.'
van: 'Schneider J, Müller U, Schwarzer K, Becker B, Wilhelm P. Lemgo Database for
D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International.
2010;(5):252–6.'
date_created: 2020-06-02T10:24:20Z
date_updated: 2023-03-15T13:49:42Z
department:
- _id: DEP4010
- _id: DEP4009
- _id: DEP4018
issue: '5'
language:
- iso: eng
page: 252 - 256
publication: Brauwelt International
publication_identifier:
issn:
- 0934-9340
publication_status: published
publisher: Fachverlag Hans Carl GmbH
status: public
title: 'Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim'
type: journal_article
user_id: '12013'
year: 2010
...
---
_id: '2763'
author:
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Patrick
full_name: Wilhelm, Patrick
id: '12013'
last_name: Wilhelm
citation:
ama: 'Schwarzer K, Schneider J, Müller U, Becker B, Wilhelm P. Lemgoer Datenbank
für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt.
2010;150(18):540-544.'
apa: 'Schwarzer, K., Schneider, J., Müller, U., Becker, B., & Wilhelm, P. (2010).
Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen.
Brauwelt, 150(18), 540–544.'
bjps: 'Schwarzer K et al. (2010) Lemgoer Datenbank Für D- Und z-Werte:
Anwendung Für Getränkeschädliche Mikroorganismen. Brauwelt 150,
540–544.'
chicago: 'Schwarzer, Knut, Jan Schneider, Ulrich Müller, Barbara Becker, and Patrick
Wilhelm. “Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für Getränkeschädliche
Mikroorganismen.” Brauwelt 150, no. 18 (2010): 540–44.'
chicago-de: 'Schwarzer, Knut, Jan Schneider, Ulrich Müller, Barbara Becker und Patrick
Wilhelm. 2010. Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche
Mikroorganismen. Brauwelt 150, Nr. 18: 540–544.'
din1505-2-1: 'Schwarzer, Knut ; Schneider, Jan ; Müller,
Ulrich ; Becker, Barbara
; Wilhelm, Patrick: Lemgoer Datenbank
für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. In: Brauwelt
Bd. 150, Fachverlag Hans Carl GmbH (2010), Nr. 18, S. 540–544'
havard: 'K. Schwarzer, J. Schneider, U. Müller, B. Becker, P. Wilhelm, Lemgoer Datenbank
für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen, Brauwelt.
150 (2010) 540–544.'
ieee: 'K. Schwarzer, J. Schneider, U. Müller, B. Becker, and P. Wilhelm, “Lemgoer
Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen,”
Brauwelt, vol. 150, no. 18, pp. 540–544, 2010.'
mla: 'Schwarzer, Knut, et al. “Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für
Getränkeschädliche Mikroorganismen.” Brauwelt, vol. 150, no. 18, 2010,
pp. 540–44.'
short: K. Schwarzer, J. Schneider, U. Müller, B. Becker, P. Wilhelm, Brauwelt 150
(2010) 540–544.
ufg: 'Schwarzer, Knut u. a.: Lemgoer Datenbank für D- und z-Werte: Anwendung
für getränkeschädliche Mikroorganismen, in: Brauwelt 150 (2010), H. 18,
S. 540–544.'
van: 'Schwarzer K, Schneider J, Müller U, Becker B, Wilhelm P. Lemgoer Datenbank
für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt.
2010;150(18):540–4.'
date_created: 2020-06-03T07:13:41Z
date_updated: 2023-03-15T13:49:42Z
department:
- _id: DEP4010
- _id: DEP4009
- _id: DEP4018
intvolume: ' 150'
issue: '18'
language:
- iso: eng
page: 540 - 544
publication: Brauwelt
publication_identifier:
issn:
- "\t0724-696X"
publication_status: published
publisher: Fachverlag Hans Carl GmbH
status: public
title: 'Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen'
type: journal_article
user_id: '12013'
volume: 150
year: 2010
...
---
_id: '2361'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Goetz
full_name: Hildebrandt, Goetz
last_name: Hildebrandt
- first_name: 'Irfan '
full_name: 'Erol, Irfan '
last_name: Erol
- first_name: Josef
full_name: Kleer, Josef
last_name: Kleer
- first_name: Aranya Sira
full_name: Manopas, Aranya Sira
last_name: Manopas
citation:
ama: Becker B, Hildebrandt G, Erol I, Kleer J, Manopas AS. Von der Döner - Prüfung
zum Döner - Heariing. Fleischwirtschaft . 2009:61-65.
apa: Becker, B., Hildebrandt, G., Erol, I., Kleer, J., & Manopas, A. S. (2009).
Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft , pp. 61–65.
bjps: Becker B et al. (2009) Von Der Döner - Prüfung Zum Döner - Heariing.
Fleischwirtschaft , 61–65.
chicago: Becker, Barbara, Goetz Hildebrandt, Irfan Erol, Josef Kleer, and Aranya
Sira Manopas. “Von Der Döner - Prüfung Zum Döner - Heariing.” Fleischwirtschaft
, 2009.
chicago-de: Becker, Barbara, Goetz Hildebrandt, Irfan Erol, Josef Kleer und Aranya
Sira Manopas. 2009. Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft
.
din1505-2-1: 'Becker, Barbara ; Hildebrandt, Goetz ; Erol,
Irfan ; Kleer, Josef ; Manopas, Aranya Sira: Von der Döner -
Prüfung zum Döner - Heariing. In: Fleischwirtschaft (2009), Nr. 7'
havard: B. Becker, G. Hildebrandt, I. Erol, J. Kleer, A.S. Manopas, Von der Döner
- Prüfung zum Döner - Heariing, Fleischwirtschaft . (2009) 61–65.
ieee: B. Becker, G. Hildebrandt, I. Erol, J. Kleer, and A. S. Manopas, “Von der
Döner - Prüfung zum Döner - Heariing,” Fleischwirtschaft , no. 7, pp. 61–65,
2009.
mla: Becker, Barbara, et al. “Von Der Döner - Prüfung Zum Döner - Heariing.” Fleischwirtschaft
, no. 7, 2009, pp. 61–65.
short: B. Becker, G. Hildebrandt, I. Erol, J. Kleer, A.S. Manopas, Fleischwirtschaft (2009)
61–65.
ufg: 'Becker, Barbara et. al. (2009): Von der Döner - Prüfung zum Döner -
Heariing, in: Fleischwirtschaft (7) (2009), S. 61–65.'
van: Becker B, Hildebrandt G, Erol I, Kleer J, Manopas AS. Von der Döner - Prüfung
zum Döner - Heariing. Fleischwirtschaft . 2009;61–5.
date_created: 2020-05-18T09:40:45Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4010
issue: '7'
language:
- iso: eng
page: 61-65
publication: 'Fleischwirtschaft '
publication_date: 19.03.2019
status: public
title: Von der Döner - Prüfung zum Döner - Heariing
type: newspaper_article
user_id: '74222'
year: 2009
...
---
_id: '6128'
author:
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
citation:
ama: 'Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase
for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der
Entkeimung. In: ; 2009.'
apa: 'Schwarzer, K., Becker, B., Schneider, J., & Müller, U. (2009). Die
Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. ProcessNet Fachausschuss Lebensmittelverfahrenstechnik,
Lausanne.'
bjps: 'Schwarzer K et al. (2009) Die Lemgo D- and z-Value Datebase
for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der
Entkeimung.'
chicago: 'Schwarzer, Knut, Barbara Becker, Jan Schneider, and Ulrich Müller. “Die
Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung
von Minimal Processing in Der Entkeimung,” 2009.'
chicago-de: 'Schwarzer, Knut, Barbara Becker, Jan Schneider und Ulrich Müller. 2009.
Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. In: .'
din1505-2-1: 'Schwarzer, Knut ; Becker, Barbara ; Schneider,
Jan ; Müller, Ulrich: Die
Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. In: , 2009'
havard: 'K. Schwarzer, B. Becker, J. Schneider, U. Müller, Die Lemgo D- and z-value
Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing
in der Entkeimung, in: 2009.'
ieee: 'K. Schwarzer, B. Becker, J. Schneider, and U. Müller, “Die Lemgo D- and z-value
Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing
in der Entkeimung,” presented at the ProcessNet Fachausschuss Lebensmittelverfahrenstechnik,
Lausanne, 2009.'
mla: 'Schwarzer, Knut, et al. Die Lemgo D- and z-Value Datebase for Food (LDzBase):
Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung. 2009.'
short: 'K. Schwarzer, B. Becker, J. Schneider, U. Müller, in: 2009.'
ufg: 'Schwarzer, Knut u. a.: Die Lemgo D- and z-value Datebase for Food (LDzBase):
Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: o. Hg.:
o. O. 2009.'
van: 'Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase
for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der
Entkeimung. In 2009.'
conference:
end_date: 2009-03-25
location: Lausanne
name: ProcessNet Fachausschuss Lebensmittelverfahrenstechnik
start_date: 2009-03-23
date_created: 2021-09-06T10:43:56Z
date_updated: 2024-05-23T11:12:12Z
department:
- _id: DEP4023
- _id: DEP4009
- _id: DEP4010
- _id: DEP4018
language:
- iso: eng
status: public
title: 'Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung'
type: conference_abstract
user_id: '83779'
year: '2009'
...
---
_id: '6141'
author:
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
citation:
ama: 'Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase
for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der
Entkeimung. In: ; 2009.'
apa: 'Schwarzer, K., Becker, B., Schneider, J., & Müller, U. (2009). Die
Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. GDL-Kongress , Lemgo.'
bjps: 'Schwarzer K et al. (2009) Die Lemgo D- and z-Value Datebase
for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der
Entkeimung.'
chicago: 'Schwarzer, Knut, Barbara Becker, Jan Schneider, and Ulrich Müller. “Die
Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung
von Minimal Processing in Der Entkeimung,” 2009.'
chicago-de: 'Schwarzer, Knut, Barbara Becker, Jan Schneider und Ulrich Müller. 2009.
Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. In: .'
din1505-2-1: 'Schwarzer, Knut ; Becker, Barbara ; Schneider,
Jan ; Müller, Ulrich: Die
Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. In: , 2009'
havard: 'K. Schwarzer, B. Becker, J. Schneider, U. Müller, Die Lemgo D- and z-value
Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing
in der Entkeimung, in: 2009.'
ieee: 'K. Schwarzer, B. Becker, J. Schneider, and U. Müller, “Die Lemgo D- and z-value
Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing
in der Entkeimung,” presented at the GDL-Kongress , Lemgo, 2009.'
mla: 'Schwarzer, Knut, et al. Die Lemgo D- and z-Value Datebase for Food (LDzBase):
Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung. 2009.'
short: 'K. Schwarzer, B. Becker, J. Schneider, U. Müller, in: 2009.'
ufg: 'Schwarzer, Knut u. a.: Die Lemgo D- and z-value Datebase for Food (LDzBase):
Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: o. Hg.:
o. O. 2009.'
van: 'Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase
for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der
Entkeimung. In 2009.'
conference:
end_date: 2009-10-24
location: Lemgo
name: 'GDL-Kongress '
start_date: 2009-10-22
date_created: 2021-09-06T11:10:55Z
date_updated: 2024-05-23T11:12:44Z
department:
- _id: DEP4023
- _id: DEP4009
- _id: DEP4010
- _id: DEP4018
language:
- iso: eng
status: public
title: 'Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung'
type: conference_abstract
user_id: '83779'
year: '2009'
...
---
_id: '12982'
abstract:
- lang: eng
text: Contaminated food is a significant vehicle for human norovirus transmission.
The present study determined the effect of physicochemical treatments on the tenacity
of infective human norovirus genogroup II in selected foods. Artificially contaminated
produce was subjected to a number of processes used by the food industry for preservation
and by the consumer for storage and preparation. Virus recovery was carried out
by using ultrafiltration and was monitored by using bacteriophage MS2 as an internal
process control. Norovirus was quantified by using monoplex one-step TaqMan real-time
reverse transcription (RT)-PCR and an external standard curve based on recombinant
RNA standards. An RNase pretreatment step was used to avoid false-positive PCR
results caused by accessible RNA, which allowed detection of intact virus particles.
Significant reductions in titers were obtained with heat treatments usually applied
by consumers for food preparation (baking, cooking, roasting). Generally, processes
used for preservation and storage, such as cooling, freezing, acidification (≥pH
4.5), and moderate heat treatments (pasteurization), appear to be insufficient
to inactivate norovirus within a food matrix or on the surface of food. Besides
data for persistence in processed food, comparable data for individual matrix-specific
protective effects, recovery rates, and inhibitory effects on the PCRs were obtained
in this study. The established procedure might be used for other noncultivable
enteric RNA viruses that are connected to food-borne diseases. The data obtained
in this study may also help optimize the process for inactivation of norovirus
in food by adjusting food processing technologies and may promote the development
of risk assessment systems in order to improve consumer protection.
author:
- first_name: Sascha
full_name: Mormann, Sascha
last_name: Mormann
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
citation:
ama: Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on
the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
Foods. Applied and environmental microbiology / American Society for Microbiology.
2009;76(2):536-545. doi:10.1128/aem.01797-09
apa: Mormann, S., Dabisch-Ruthe, M., & Becker, B. (2009). Effects of Technological
Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally
Contaminated Foods. Applied and Environmental Microbiology / American Society
for Microbiology, 76(2), 536–545. https://doi.org/10.1128/aem.01797-09
bjps: Mormann S, Dabisch-Ruthe M and Becker B (2009) Effects of Technological
Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally
Contaminated Foods. Applied and environmental microbiology / American Society
for Microbiology 76, 536–545.
chicago: 'Mormann, Sascha, Mareike Dabisch-Ruthe, and Barbara Becker. “Effects of
Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
II in Experimentally Contaminated Foods.” Applied and Environmental Microbiology
/ American Society for Microbiology 76, no. 2 (2009): 536–45. https://doi.org/10.1128/aem.01797-09.'
chicago-de: 'Mormann, Sascha, Mareike Dabisch-Ruthe und Barbara Becker. 2009. Effects
of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
II in Experimentally Contaminated Foods. Applied and environmental microbiology
/ American Society for Microbiology 76, Nr. 2: 536–545. doi:10.1128/aem.01797-09,
.'
din1505-2-1: 'Mormann, Sascha ; Dabisch-Ruthe, Mareike ; Becker,
Barbara: Effects of Technological Processes on the Tenacity and Inactivation
of Norovirus Genogroup II in Experimentally Contaminated Foods. In: Applied
and environmental microbiology / American Society for Microbiology Bd. 76.
Washington, DC [u.a.], American Society for Microbiology (2009), Nr. 2, S. 536–545'
havard: S. Mormann, M. Dabisch-Ruthe, B. Becker, Effects of Technological Processes
on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
Foods, Applied and Environmental Microbiology / American Society for Microbiology.
76 (2009) 536–545.
ieee: 'S. Mormann, M. Dabisch-Ruthe, and B. Becker, “Effects of Technological Processes
on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
Foods,” Applied and environmental microbiology / American Society for Microbiology,
vol. 76, no. 2, pp. 536–545, 2009, doi: 10.1128/aem.01797-09.'
mla: Mormann, Sascha, et al. “Effects of Technological Processes on the Tenacity
and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods.”
Applied and Environmental Microbiology / American Society for Microbiology,
vol. 76, no. 2, 2009, pp. 536–45, https://doi.org/10.1128/aem.01797-09.
short: S. Mormann, M. Dabisch-Ruthe, B. Becker, Applied and Environmental Microbiology
/ American Society for Microbiology 76 (2009) 536–545.
ufg: 'Mormann, Sascha/Dabisch-Ruthe, Mareike/Becker, Barbara: Effects of
Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
II in Experimentally Contaminated Foods, in: Applied and environmental microbiology
/ American Society for Microbiology 76 (2009), H. 2, S. 536–545.'
van: Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on
the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
Foods. Applied and environmental microbiology / American Society for Microbiology.
2009;76(2):536–45.
date_created: 2025-06-17T06:26:59Z
date_updated: 2025-06-17T13:49:35Z
department:
- _id: DEP4010
doi: 10.1128/aem.01797-09
intvolume: ' 76'
issue: '2'
language:
- iso: eng
page: 536-545
place: Washington, DC [u.a.]
publication: ' Applied and environmental microbiology / American Society for Microbiology'
publication_identifier:
eissn:
- 1098-5336
issn:
- '0099-2240 '
publication_status: published
publisher: American Society for Microbiology
quality_controlled: '1'
status: public
title: Effects of Technological Processes on the Tenacity and Inactivation of Norovirus
Genogroup II in Experimentally Contaminated Foods
type: scientific_journal_article
user_id: '83781'
volume: 76
year: '2009'
...
---
_id: '2359'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
citation:
ama: 'Becker B. Lebensmittelassoziierte Viren - aktuelle Apekte. In: ; 2004.'
apa: Becker, B. (2004). Lebensmittelassoziierte Viren - aktuelle Apekte. Presented
at the Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie.
bjps: Becker B (2004) Lebensmittelassoziierte Viren - Aktuelle Apekte.
chicago: Becker, Barbara. “Lebensmittelassoziierte Viren - Aktuelle Apekte,” 2004.
chicago-de: 'Becker, Barbara. 2004. Lebensmittelassoziierte Viren - aktuelle Apekte.
In: .'
din1505-2-1: 'Becker, Barbara: Lebensmittelassoziierte
Viren - aktuelle Apekte. In: , 2004'
havard: 'B. Becker, Lebensmittelassoziierte Viren - aktuelle Apekte, in: 2004.'
ieee: B. Becker, “Lebensmittelassoziierte Viren - aktuelle Apekte,” presented at
the Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie, 2004.
mla: Becker, Barbara. Lebensmittelassoziierte Viren - Aktuelle Apekte. 2004.
short: 'B. Becker, in: 2004.'
ufg: 'Becker, Barbara (2004): Lebensmittelassoziierte Viren - aktuelle Apekte,
in: .'
van: Becker B. Lebensmittelassoziierte Viren - aktuelle Apekte. In 2004.
conference:
end_date: 16.07.2004
name: Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie
start_date: 14.07.2004
date_created: 2020-05-18T09:33:00Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4010
language:
- iso: eng
status: public
title: Lebensmittelassoziierte Viren - aktuelle Apekte
type: conference
user_id: '74222'
year: 2004
...