---
_id: '2363'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
citation:
ama: Becker B. useful for beverage spoilage microoganism. Brauwelt International.
2014:252-256.
apa: Becker, B. (2014). useful for beverage spoilage microoganism. Brauwelt International,
pp. 252–256.
bjps: Becker B (2014) Useful for Beverage Spoilage Microoganism. Brauwelt
International, 252–256.
chicago: Becker, Barbara. “Useful for Beverage Spoilage Microoganism.” Brauwelt
International, 2014.
chicago-de: Becker, Barbara. 2014. useful for beverage spoilage microoganism. Brauwelt
International.
din1505-2-1: 'Becker, Barbara: useful
for beverage spoilage microoganism. In: Brauwelt International (2014),
Nr. 5'
havard: B. Becker, useful for beverage spoilage microoganism, Brauwelt International.
(2014) 252–256.
ieee: B. Becker, “useful for beverage spoilage microoganism,” Brauwelt International,
no. 5, pp. 252–256, 2014.
mla: Becker, Barbara. “Useful for Beverage Spoilage Microoganism.” Brauwelt International,
no. 5, 2014, pp. 252–56.
short: B. Becker, Brauwelt International (2014) 252–256.
ufg: 'Becker, Barbara (2014): useful for beverage spoilage microoganism,
in: Brauwelt International (5) (2014), S. 252–256.'
van: Becker B. useful for beverage spoilage microoganism. Brauwelt International.
2014;252–6.
date_created: 2020-05-18T10:12:34Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4010
issue: '5'
language:
- iso: eng
page: 252-256
publication: Brauwelt International
publication_date: '2014'
publication_identifier:
unknown:
- 0934-9340
status: public
title: useful for beverage spoilage microoganism
type: newspaper_article
user_id: '74222'
year: 2014
...
---
_id: '2403'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Verena
full_name: Becker, Verena
last_name: Becker
- first_name: Heiko
full_name: Nerenz, Heiko
last_name: Nerenz
- first_name: Andreas
full_name: Schrader, Andreas
last_name: Schrader
citation:
ama: 'Becker B, Becker V, Nerenz H, Schrader A. Einsatz der Durchflusszytometrie
zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten.
SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe. 2014;140(5):26-27, 30.'
apa: 'Becker, B., Becker, V., Nerenz, H., & Schrader, A. (2014). Einsatz der
Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen
und Pflanzenextrakten. SOFW-Journal : Home & Personal Care Ingredients
& Formulations; Deutsche Ausgabe, 140(5), 26–27, 30.'
bjps: 'Becker B et al. (2014) Einsatz Der Durchflusszytometrie Zum
Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten.
SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe 140, 26–27, 30.'
chicago: 'Becker, Barbara, Verena Becker, Heiko Nerenz, and Andreas Schrader. “Einsatz
Der Durchflusszytometrie Zum Nachweis Der Antimikrobiellen Wirkung von Phytoalexinen
Und Pflanzenextrakten.” SOFW-Journal : Home & Personal Care Ingredients
& Formulations; Deutsche Ausgabe 140, no. 5 (2014): 26–27, 30.'
chicago-de: 'Becker, Barbara, Verena Becker, Heiko Nerenz und Andreas Schrader.
2014. Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung
von Phytoalexinen und Pflanzenextrakten. SOFW-Journal : Home & personal
care ingredients & formulations; Deutsche Ausgabe 140, Nr. 5: 26–27, 30.'
din1505-2-1: 'Becker, Barbara ; Becker, Verena ; Nerenz,
Heiko ; Schrader, Andreas:
Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung von
Phytoalexinen und Pflanzenextrakten. In: SOFW-Journal : Home & personal
care ingredients & formulations; Deutsche Ausgabe Bd. 140, Verl. für Chemische
Industrie, Ziolkowsky (2014), Nr. 5, S. 26–27, 30'
havard: 'B. Becker, V. Becker, H. Nerenz, A. Schrader, Einsatz der Durchflusszytometrie
zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten,
SOFW-Journal : Home & Personal Care Ingredients & Formulations; Deutsche
Ausgabe. 140 (2014) 26–27, 30.'
ieee: 'B. Becker, V. Becker, H. Nerenz, and A. Schrader, “Einsatz der Durchflusszytometrie
zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten,”
SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe, vol. 140, no. 5, pp. 26–27, 30, 2014.'
mla: 'Becker, Barbara, et al. “Einsatz Der Durchflusszytometrie Zum Nachweis Der
Antimikrobiellen Wirkung von Phytoalexinen Und Pflanzenextrakten.” SOFW-Journal :
Home & Personal Care Ingredients & Formulations; Deutsche Ausgabe,
vol. 140, no. 5, 2014, pp. 26–27, 30.'
short: 'B. Becker, V. Becker, H. Nerenz, A. Schrader, SOFW-Journal : Home &
Personal Care Ingredients & Formulations; Deutsche Ausgabe 140 (2014) 26–27,
30.'
ufg: 'Becker, Barbara u. a.: Einsatz der Durchflusszytometrie zum Nachweis
der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten, in: SOFW-Journal :
Home & personal care ingredients & formulations; Deutsche Ausgabe
140 (2014), H. 5, S. 26–27, 30.'
van: 'Becker B, Becker V, Nerenz H, Schrader A. Einsatz der Durchflusszytometrie
zum Nachweis der antimikrobiellen Wirkung von Phytoalexinen und Pflanzenextrakten.
SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe. 2014;140(5):26–7, 30.'
date_created: 2020-05-20T07:28:39Z
date_updated: 2024-12-02T14:21:46Z
ddc:
- '610'
- '660'
department:
- _id: DEP4010
file:
- access_level: closed
content_type: application/pdf
creator: z5h-0dr
date_created: 2020-05-20T07:37:08Z
date_updated: 2024-12-02T14:21:45Z
file_id: '2405'
file_name: becker(2).pdf
file_size: 3196553
relation: main_file
file_date_updated: 2024-12-02T14:21:45Z
has_accepted_license: '1'
intvolume: ' 140'
issue: '5'
language:
- iso: eng
page: 26 - 27, 30
publication: 'SOFW-Journal : Home & personal care ingredients & formulations; Deutsche
Ausgabe'
publication_identifier:
issn:
- 0942-7694
publication_status: published
publisher: Verl. für Chemische Industrie, Ziolkowsky
status: public
title: Einsatz der Durchflusszytometrie zum Nachweis der antimikrobiellen Wirkung
von Phytoalexinen und Pflanzenextrakten
type: journal_article
user_id: '83781'
volume: 140
year: '2014'
...
---
_id: '12979'
abstract:
- lang: eng
text: Physiological consequences of adaptation to and continued feeding of a high-energetic
diet were studied in eight non-pregnant, non-lactating dairy Holstein cows over
a period of 16 weeks. The first six weeks served as an adaptation period from
the low energetic straw-based diet (3.8 MJ NEL/kg DM) to the high-energetic ration
(7.5 MJ NEL/kg DM). Intake of dry matter (DM) increased with dietary energy concentration
from 9 to 20 kg/d up to week 9 to 12 and decreased thereafter. The initial live
weight (LW) of 550 ± 60 kg was increased linearly and corresponded to an average
daily LW gain of 2.3 ± 0.3 kg. Energy balance increased approximately nine-fold
to a maximum of 114 MJ NEL/d in week 10. Ruminal fermentation pattern was completely
changed from an acetate dominating profile to a propionate based one, which was
paralleled by a marked increase in the rumen fluid endotoxin concentration. Unlike
blood glucose concentration, which increased continuously, that of cholesterol
and triglycerides started to increase after an initial stagnation. In conclusion,
both ruminal adaptation to a high-energetic diet and the continued feeding of
such a diet induced digestive and metabolic adaptations in non-pregnant, non-lactating
cows characterised by a progressing positive energy balance.
author:
- first_name: Sven
full_name: Dänicke, Sven
last_name: Dänicke
- first_name: Ulrich
full_name: Meyer, Ulrich
last_name: Meyer
- first_name: Janine
full_name: Winkler, Janine
last_name: Winkler
- first_name: Kirsten
full_name: Schulz, Kirsten
last_name: Schulz
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Jana
full_name: Frahm, Jana
last_name: Frahm
- first_name: Susanne
full_name: Kersten, Susanne
last_name: Kersten
- first_name: Jürgen
full_name: Rehage, Jürgen
last_name: Rehage
- first_name: Gerhard
full_name: Breves, Gerhard
last_name: Breves
- first_name: Susanne
full_name: Häußler, Susanne
last_name: Häußler
- first_name: Helga
full_name: Sauerwein, Helga
last_name: Sauerwein
- first_name: Lena
full_name: Locher, Lena
last_name: Locher
citation:
ama: Dänicke S, Meyer U, Winkler J, et al. Description of a bovine model for studying
digestive and metabolic effects of a positive energy balance not biased by lactation
or gravidity. Archives of Animal Nutrition. 2014;68(6):460-477. doi:10.1080/1745039x.2014.973243
apa: Dänicke, S., Meyer, U., Winkler, J., Schulz, K., Ulrich, S., Frahm, J., Kersten,
S., Rehage, J., Breves, G., Häußler, S., Sauerwein, H., & Locher, L. (2014).
Description of a bovine model for studying digestive and metabolic effects of
a positive energy balance not biased by lactation or gravidity. Archives of
Animal Nutrition, 68(6), 460–477. https://doi.org/10.1080/1745039x.2014.973243
bjps: Dänicke S et al. (2014) Description of a Bovine Model for Studying
Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation
or Gravidity. Archives of Animal Nutrition 68, 460–477.
chicago: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian
Ulrich, Jana Frahm, Susanne Kersten, et al. “Description of a Bovine Model for
Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased
by Lactation or Gravidity.” Archives of Animal Nutrition 68, no. 6 (2014):
460–77. https://doi.org/10.1080/1745039x.2014.973243.'
chicago-de: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian
Ulrich, Jana Frahm, Susanne Kersten, u. a. 2014. Description of a bovine model
for studying digestive and metabolic effects of a positive energy balance not
biased by lactation or gravidity. Archives of Animal Nutrition 68, Nr.
6: 460–477. doi:10.1080/1745039x.2014.973243,
.'
din1505-2-1: 'Dänicke,
Sven ; Meyer, Ulrich ; Winkler, Janine ; Schulz,
Kirsten ; Ulrich, Sebastian
; Frahm, Jana ; Kersten,
Susanne ; Rehage, Jürgen
; u. a.: Description of a bovine model for studying digestive and metabolic
effects of a positive energy balance not biased by lactation or gravidity. In:
Archives of Animal Nutrition Bd. 68. Abingdon, Taylor & Francis (2014),
Nr. 6, S. 460–477'
havard: S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten,
J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Description of a bovine
model for studying digestive and metabolic effects of a positive energy balance
not biased by lactation or gravidity, Archives of Animal Nutrition. 68 (2014)
460–477.
ieee: 'S. Dänicke et al., “Description of a bovine model for studying digestive
and metabolic effects of a positive energy balance not biased by lactation or
gravidity,” Archives of Animal Nutrition, vol. 68, no. 6, pp. 460–477,
2014, doi: 10.1080/1745039x.2014.973243.'
mla: Dänicke, Sven, et al. “Description of a Bovine Model for Studying Digestive
and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or
Gravidity.” Archives of Animal Nutrition, vol. 68, no. 6, 2014, pp. 460–77,
https://doi.org/10.1080/1745039x.2014.973243.
short: S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten,
J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Archives of Animal
Nutrition 68 (2014) 460–477.
ufg: 'Dänicke, Sven u. a.: Description of a bovine model for studying digestive
and metabolic effects of a positive energy balance not biased by lactation or
gravidity, in: Archives of Animal Nutrition 68 (2014), H. 6, S. 460–477.'
van: Dänicke S, Meyer U, Winkler J, Schulz K, Ulrich S, Frahm J, et al. Description
of a bovine model for studying digestive and metabolic effects of a positive energy
balance not biased by lactation or gravidity. Archives of Animal Nutrition. 2014;68(6):460–77.
date_created: 2025-06-15T10:36:14Z
date_updated: 2025-06-18T12:23:01Z
department:
- _id: DEP4010
doi: 10.1080/1745039x.2014.973243
extern: '1'
intvolume: ' 68'
issue: '6'
keyword:
- blood chemistry
- dairy cows
- endotoxins
- energy balance
- energy content
- rumen fermentation
language:
- iso: eng
page: 460-477
place: Abingdon
publication: Archives of Animal Nutrition
publication_identifier:
eissn:
- 1477-2817
issn:
- 1745-039X
- 0003-942X
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
status: public
title: Description of a bovine model for studying digestive and metabolic effects
of a positive energy balance not biased by lactation or gravidity
type: scientific_journal_article
user_id: '83781'
volume: 68
year: '2014'
...
---
_id: '12984'
abstract:
- lang: eng
text: "Background\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder
characterized by late onset and progressive calcification of elastic fibers in
skin, eyes and the cardiovascular system, exemplifying a model for conditions
characterized by soft tissue calcification.\r\nObjective\r\nThe aim of our study
was to characterize cellular inorganic pyrophosphate (PPi) homeostasis in PXE.\r\nMethods\r\nGene
expression of PPi metabolizing enzymes was determined by quantitative real-time
PCR after incubation up to 21 days with or without addition of Na2HPO4. Extracellular
and cytosolic PPi concentrations were measured by enzyme-linked bioluminescence
assay. ALP and ENPP1 activity was determined spectrophotometrically. We further
established a human cell culture model suitable for investigating PXE and related
disorders without addition of artificial calcification triggers.\r\nResults\r\nIndependently
of the experimental conditions, PXE fibroblasts revealed a higher degree of matrix
calcification. We observed that matrix calcification was associated with altered
gene expression of PPi metabolizing enzymes in PXE fibroblasts. In this context,
PXE fibroblasts exhibited significantly higher expression of ALP and OPN and reduced
mRNA expression and activity of ENPP1. Here, for the first time cytosolic and
extracellular PPi levels were shown to be strongly reduced in PXE fibroblasts.
We further showed that PPi concentration in bovine and human sera additives had
a strong impact on matrix calcification. In a last experimental line, we demonstrated
that addition of PPi analogs reduced matrix calcification of PXE fibroblasts most
likely by reducing ALP and OPN mRNA expression, restoring ENPP1 activity and subsequently
elevating PPi concentrations.\r\nConclusion\r\nThe results of our study along
with recent findings point to the essential role of PPi as the central regulatory
metabolites preventing matrix calcification in PXE. But what remains to be determined
is the underlying molecular mechanism leading to depletion of PPi in PXE. We further
suggest that supplementation of PPi analogs might counteract pathological calcification
in PXE and related disorders."
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Patricia
full_name: Kuzaj, Patricia
last_name: Kuzaj
- first_name: Christian
full_name: Götting, Christian
last_name: Götting
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Doris
full_name: Hendig, Doris
last_name: Hendig
citation:
ama: Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as
a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal
of Dermatological Science. 2014;75(2):109-120. doi:10.1016/j.jdermsci.2014.04.015
apa: Dabisch-Ruthe, M., Kuzaj, P., Götting, C., Knabbe, C., & Hendig, D. (2014).
Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma
elasticum. Journal of Dermatological Science, 75(2), 109–120. https://doi.org/10.1016/j.jdermsci.2014.04.015
bjps: Dabisch-Ruthe M et al. (2014) Pyrophosphates as a Major Inhibitor
of Matrix Calcification in Pseudoxanthoma Elasticum. Journal of Dermatological
Science 75, 109–120.
chicago: 'Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius Knabbe,
and Doris Hendig. “Pyrophosphates as a Major Inhibitor of Matrix Calcification
in Pseudoxanthoma Elasticum.” Journal of Dermatological Science 75, no.
2 (2014): 109–20. https://doi.org/10.1016/j.jdermsci.2014.04.015.'
chicago-de: 'Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius
Knabbe und Doris Hendig. 2014. Pyrophosphates as a major inhibitor of matrix calcification
in Pseudoxanthoma elasticum. Journal of Dermatological Science 75, Nr.
2: 109–120. doi:10.1016/j.jdermsci.2014.04.015,
.'
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Kuzaj, Patricia ; Götting,
Christian ; Knabbe, Cornelius
; Hendig, Doris: Pyrophosphates
as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. In:
Journal of Dermatological Science Bd. 75. Amsterdam, Elsevier (2014),
Nr. 2, S. 109–120'
havard: M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Pyrophosphates
as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum, Journal
of Dermatological Science. 75 (2014) 109–120.
ieee: 'M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, and D. Hendig, “Pyrophosphates
as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum,” Journal
of Dermatological Science, vol. 75, no. 2, pp. 109–120, 2014, doi: 10.1016/j.jdermsci.2014.04.015.'
mla: Dabisch-Ruthe, Mareike, et al. “Pyrophosphates as a Major Inhibitor of Matrix
Calcification in Pseudoxanthoma Elasticum.” Journal of Dermatological Science,
vol. 75, no. 2, 2014, pp. 109–20, https://doi.org/10.1016/j.jdermsci.2014.04.015.
short: M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Journal of
Dermatological Science 75 (2014) 109–120.
ufg: 'Dabisch-Ruthe, Mareike u. a.: Pyrophosphates as a major inhibitor of
matrix calcification in Pseudoxanthoma elasticum, in: Journal of Dermatological
Science 75 (2014), H. 2, S. 109–120.'
van: Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as
a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal
of Dermatological Science. 2014;75(2):109–20.
date_created: 2025-06-17T06:52:59Z
date_updated: 2025-06-17T13:48:41Z
department:
- _id: DEP4010
doi: 10.1016/j.jdermsci.2014.04.015
intvolume: ' 75'
issue: '2'
keyword:
- Pseudoxanthoma elasticum
- Calcification
- Pyrophosphate
- Bisphosphonate
- ABCC6
- Tissue nonspecific alkaline phosphate
- Ectonucleotide pyrophosphatase 1
language:
- iso: eng
page: 109-120
place: Amsterdam
publication: Journal of Dermatological Science
publication_identifier:
eissn:
- 1873-569X
issn:
- 0923-1811
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma
elasticum
type: scientific_journal_article
user_id: '83781'
volume: 75
year: '2014'
...
---
_id: '12985'
abstract:
- lang: eng
text: "Objectives\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder
characterized by progressive calcification and fragmentation of elastic fibers.
Because of the great clinical variability between PXE patients the involvement
of modifier genes was recently suggested. Therefore, we investigated the association
of single nucleotide variants (SNVs) in selected candidate genes known to regulate
cellular pyrophosphate metabolism.\r\nDesign and methods\r\nWe used RLFP analyses
to evaluate the distribution of SNVs in alkaline phosphatase (ALP), ectonucleotide
pyrophosphatase 1 (ENPP1) and ankylosis (ANKH) in DNA samples from 190 German
PXE patients and 190 age- and sex-matched healthy controls. Statistical analyses
were performed using Fisher exact test and Bonferroni correction.\r\nResults\r\nThe
screening revealed three different SNVs in three genes, which were associated
with PXE. The SNV c.1190-65C > A (rs1780329, minor allele frequency (MAF) patients:
0.17; controls: 0.11; P = 0.04) in the ALP gene was significantly more frequent
in PXE patients. Furthermore, PXE was highly associated with ANKH p.A98A genotype
TT (P = 0.0012), although the MAF was not different between patients and controls.
After correction for multiple testing according to the Bonferroni method, one
SNV in the ENPP1 gene (c.313 + 9G > T, rs7773477) remained significantly associated
with PXE with significantly higher MAF values in the patient cohort (MAF: 0.04
vs. 0.00; P = 0.0024) and a high association with PXE susceptibility (OR 27.96).\r\nConclusion\r\nPolymorphisms
in ALP, ENPP1 and ANKH are important genetic risk factors contributing to PXE."
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Alexander
full_name: Brock, Alexander
last_name: Brock
- first_name: Patricia
full_name: Kuzaj, Patricia
last_name: Kuzaj
- first_name: Peter
full_name: Charbel Issa, Peter
last_name: Charbel Issa
- first_name: Christiane
full_name: Szliska, Christiane
last_name: Szliska
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Doris
full_name: Hendig, Doris
last_name: Hendig
citation:
ama: Dabisch-Ruthe M, Brock A, Kuzaj P, et al. Variants in genes encoding pyrophosphate
metabolizing enzymes are associated with Pseudoxanthoma elasticum. Clinical
Biochemistry. 2014;47(15):60-67. doi:10.1016/j.clinbiochem.2014.07.003
apa: Dabisch-Ruthe, M., Brock, A., Kuzaj, P., Charbel Issa, P., Szliska, C., Knabbe,
C., & Hendig, D. (2014). Variants in genes encoding pyrophosphate metabolizing
enzymes are associated with Pseudoxanthoma elasticum. Clinical Biochemistry,
47(15), 60–67. https://doi.org/10.1016/j.clinbiochem.2014.07.003
bjps: Dabisch-Ruthe M et al. (2014) Variants in Genes Encoding Pyrophosphate
Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum. Clinical
Biochemistry 47, 60–67.
chicago: 'Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel
Issa, Christiane Szliska, Cornelius Knabbe, and Doris Hendig. “Variants in Genes
Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma
Elasticum.” Clinical Biochemistry 47, no. 15 (2014): 60–67. https://doi.org/10.1016/j.clinbiochem.2014.07.003.'
chicago-de: 'Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel
Issa, Christiane Szliska, Cornelius Knabbe und Doris Hendig. 2014. Variants in
genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma
elasticum. Clinical Biochemistry 47, Nr. 15: 60–67. doi:10.1016/j.clinbiochem.2014.07.003,
.'
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Brock, Alexander ; Kuzaj,
Patricia ; Charbel Issa, Peter
; Szliska, Christiane ; Knabbe,
Cornelius ; Hendig, Doris:
Variants in genes encoding pyrophosphate metabolizing enzymes are associated with
Pseudoxanthoma elasticum. In: Clinical Biochemistry Bd. 47. Amsterdam,
Elsevier (2014), Nr. 15, S. 60–67'
havard: M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe,
D. Hendig, Variants in genes encoding pyrophosphate metabolizing enzymes are associated
with Pseudoxanthoma elasticum, Clinical Biochemistry. 47 (2014) 60–67.
ieee: 'M. Dabisch-Ruthe et al., “Variants in genes encoding pyrophosphate
metabolizing enzymes are associated with Pseudoxanthoma elasticum,” Clinical
Biochemistry, vol. 47, no. 15, pp. 60–67, 2014, doi: 10.1016/j.clinbiochem.2014.07.003.'
mla: Dabisch-Ruthe, Mareike, et al. “Variants in Genes Encoding Pyrophosphate Metabolizing
Enzymes Are Associated with Pseudoxanthoma Elasticum.” Clinical Biochemistry,
vol. 47, no. 15, 2014, pp. 60–67, https://doi.org/10.1016/j.clinbiochem.2014.07.003.
short: M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe,
D. Hendig, Clinical Biochemistry 47 (2014) 60–67.
ufg: 'Dabisch-Ruthe, Mareike u. a.: Variants in genes encoding pyrophosphate
metabolizing enzymes are associated with Pseudoxanthoma elasticum, in: Clinical
Biochemistry 47 (2014), H. 15, S. 60–67.'
van: Dabisch-Ruthe M, Brock A, Kuzaj P, Charbel Issa P, Szliska C, Knabbe C, et
al. Variants in genes encoding pyrophosphate metabolizing enzymes are associated
with Pseudoxanthoma elasticum. Clinical Biochemistry. 2014;47(15):60–7.
date_created: 2025-06-17T06:53:30Z
date_updated: 2025-06-17T13:48:52Z
department:
- _id: DEP4010
doi: 10.1016/j.clinbiochem.2014.07.003
intvolume: ' 47'
issue: '15'
keyword:
- Pseudoxanthoma elasticum
- Calcification
- Tissue nonspecific alkaline phosphatase
- Ectonucleotide pyrophosphatase 1
- Ankylosis
language:
- iso: eng
page: 60-67
place: Amsterdam
publication: Clinical Biochemistry
publication_identifier:
eissn:
- 1873-2933
issn:
- 0009-9120
publication_status: published
publisher: Elsevier
status: public
title: Variants in genes encoding pyrophosphate metabolizing enzymes are associated
with Pseudoxanthoma elasticum
type: scientific_journal_article
user_id: '83781'
volume: 47
year: '2014'
...
---
_id: '12986'
abstract:
- lang: eng
text: "Background\r\nDysregulations in cholesterol and lipid metabolism have been
linked to human diseases like hypercholesterolemia, atherosclerosis or the metabolic
syndrome. Many ABC transporters are involved in trafficking of metabolites derived
from these pathways. Pseudoxanthoma elasticum (PXE), an autosomal-recessive disease
caused by ABCC6 mutations, is characterized by atherogenesis and soft tissue calcification.\r\nMethods\r\nIn
this study we investigated the regulation of cholesterol biosynthesis in human
dermal fibroblasts from PXE patients and healthy controls.\r\nResults\r\nGene
expression analysis of 84 targets indicated dysregulations in cholesterol metabolism
in PXE fibroblasts. Transcript levels of ABCC6 were strongly increased in lipoprotein-deficient
serum (LPDS) and under serum starvation in healthy controls. For the first time,
increased HMG CoA reductase activities were found in PXE fibroblasts. We further
observed strongly elevated transcript and protein levels for the proprotein convertase
subtilisin/kexin type 9 (PCSK9), as well as a significant reduction in APOE mRNA
expression in PXE.\r\nConclusion\r\nIncreased cholesterol biosynthesis, elevated
PCSK9 levels and reduced APOE mRNA expression newly found in PXE fibroblasts could
enforce atherogenesis and cardiovascular risk in PXE patients. Moreover, the increase
in ABCC6 expression accompanied by the induction of cholesterol biosynthesis supposes
a functional role for ABCC6 in human lipoprotein and cholesterol homeostasis."
article_number: '118'
author:
- first_name: Patricia
full_name: Kuzaj, Patricia
last_name: Kuzaj
- first_name: Joachim
full_name: Kuhn, Joachim
last_name: Kuhn
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Isabel
full_name: Faust, Isabel
last_name: Faust
- first_name: Christian
full_name: Götting, Christian
last_name: Götting
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Doris
full_name: Hendig, Doris
last_name: Hendig
citation:
ama: Kuzaj P, Kuhn J, Dabisch-Ruthe M, et al. ABCC6- a new player in cellular cholesterol
and lipoprotein metabolism? Lipids in Health and Disease. 2014;13(1). doi:10.1186/1476-511x-13-118
apa: Kuzaj, P., Kuhn, J., Dabisch-Ruthe, M., Faust, I., Götting, C., Knabbe, C.,
& Hendig, D. (2014). ABCC6- a new player in cellular cholesterol and lipoprotein
metabolism? Lipids in Health and Disease, 13(1), Article 118. https://doi.org/10.1186/1476-511x-13-118
bjps: Kuzaj P et al. (2014) ABCC6- a New Player in Cellular Cholesterol
and Lipoprotein Metabolism? Lipids in Health and Disease 13.
chicago: Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust, Christian
Götting, Cornelius Knabbe, and Doris Hendig. “ABCC6- a New Player in Cellular
Cholesterol and Lipoprotein Metabolism?” Lipids in Health and Disease 13,
no. 1 (2014). https://doi.org/10.1186/1476-511x-13-118.
chicago-de: Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust,
Christian Götting, Cornelius Knabbe und Doris Hendig. 2014. ABCC6- a new player
in cellular cholesterol and lipoprotein metabolism? Lipids in Health and Disease
13, Nr. 1. doi:10.1186/1476-511x-13-118,
.
din1505-2-1: 'Kuzaj, Patricia ; Kuhn, Joachim ; Dabisch-Ruthe,
Mareike ; Faust, Isabel ;
Götting, Christian ; Knabbe,
Cornelius ; Hendig, Doris:
ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? In: Lipids
in Health and Disease Bd. 13. London, Biomed Central (2014), Nr. 1'
havard: P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D.
Hendig, ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?,
Lipids in Health and Disease. 13 (2014).
ieee: 'P. Kuzaj et al., “ABCC6- a new player in cellular cholesterol and
lipoprotein metabolism?,” Lipids in Health and Disease, vol. 13, no. 1,
Art. no. 118, 2014, doi: 10.1186/1476-511x-13-118.'
mla: Kuzaj, Patricia, et al. “ABCC6- a New Player in Cellular Cholesterol and Lipoprotein
Metabolism?” Lipids in Health and Disease, vol. 13, no. 1, 118, 2014, https://doi.org/10.1186/1476-511x-13-118.
short: P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D.
Hendig, Lipids in Health and Disease 13 (2014).
ufg: 'Kuzaj, Patricia u. a.: ABCC6- a new player in cellular cholesterol
and lipoprotein metabolism?, in: Lipids in Health and Disease 13 (2014),
H. 1.'
van: Kuzaj P, Kuhn J, Dabisch-Ruthe M, Faust I, Götting C, Knabbe C, et al. ABCC6-
a new player in cellular cholesterol and lipoprotein metabolism? Lipids in Health
and Disease. 2014;13(1).
date_created: 2025-06-17T06:53:58Z
date_updated: 2025-06-17T13:49:15Z
department:
- _id: DEP4010
doi: 10.1186/1476-511x-13-118
intvolume: ' 13'
issue: '1'
keyword:
- Pseudoxanthoma elasticum
- ABC transporter
- ABCC6
- Cholesterol biosynthesis
- Atherosclerosis
- HMG CoA reductase
- SREBP2
- PCSK9
- LDLR
- APOE
language:
- iso: eng
place: London
publication: Lipids in Health and Disease
publication_identifier:
eissn:
- 1476-511X
publication_status: published
publisher: 'Biomed Central '
quality_controlled: '1'
status: public
title: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?
type: scientific_journal_article
user_id: '83781'
volume: 13
year: '2014'
...
---
_id: '12987'
abstract:
- lang: eng
text: Mutations in the ABC transporter ABCC6 were recently identified as cause of
Pseudoxanthoma elasticum (PXE), a rare genetic disorder characterized by progressive
mineralization of elastic fibers. We used an untargeted metabolic approach to
identify biochemical differences between human dermal fibroblasts from healthy
controls and PXE patients in an attempt to find a link between ABCC6 deficiency,
cellular metabolic alterations and disease pathogenesis. 358 compounds were identified
by mass spectrometry covering lipids, amino acids, peptides, carbohydrates, nucleotides,
vitamins and cofactors, xenobiotics and energy metabolites. We found substantial
differences in glycerophospholipid composition, leucine dipeptides, and polypeptides
as well as alterations in pantothenate and guanine metabolism to be significantly
associated with PXE pathogenesis. These findings can be linked to extracellular
matrix remodeling and increased oxidative stress, which reflect characteristic
hallmarks of PXE. Our study could facilitate a better understanding of biochemical
pathways involved in soft tissue mineralization.
article_number: e108336
author:
- first_name: Patricia
full_name: Kuzaj, Patricia
last_name: Kuzaj
- first_name: Joachim
full_name: Kuhn, Joachim
last_name: Kuhn
- first_name: Ryan D.
full_name: Michalek, Ryan D.
last_name: Michalek
- first_name: Edward D.
full_name: Karoly, Edward D.
last_name: Karoly
- first_name: Isabel
full_name: Faust, Isabel
last_name: Faust
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Doris
full_name: Hendig, Doris
last_name: Hendig
citation:
ama: Kuzaj P, Kuhn J, Michalek RD, et al. Large-Scaled Metabolic Profiling of Human
Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
Controls. PLOS ONE / Public Library of Science . 2014;9(9). doi:10.1371/journal.pone.0108336
apa: Kuzaj, P., Kuhn, J., Michalek, R. D., Karoly, E. D., Faust, I., Dabisch-Ruthe,
M., Knabbe, C., & Hendig, D. (2014). Large-Scaled Metabolic Profiling of Human
Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
Controls. PLOS ONE / Public Library of Science , 9(9), Article e108336.
https://doi.org/10.1371/journal.pone.0108336
bjps: Kuzaj P et al. (2014) Large-Scaled Metabolic Profiling of Human
Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
Controls. PLOS ONE / Public Library of Science 9.
chicago: Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel
Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe, and Doris Hendig. “Large-Scaled
Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
Patients and Healthy Controls.” PLOS ONE / Public Library of Science 9,
no. 9 (2014). https://doi.org/10.1371/journal.pone.0108336.
chicago-de: Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel
Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe und Doris Hendig. 2014. Large-Scaled
Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
Patients and Healthy Controls. PLOS ONE / Public Library of Science 9,
Nr. 9. doi:10.1371/journal.pone.0108336,
.
din1505-2-1: 'Kuzaj, Patricia ; Kuhn, Joachim ; Michalek,
Ryan D. ; Karoly, Edward D.
; Faust, Isabel ; Dabisch-Ruthe,
Mareike ; Knabbe, Cornelius
; Hendig, Doris: Large-Scaled Metabolic
Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients
and Healthy Controls. In: PLOS ONE / Public Library of Science Bd. 9.
San Francisco, California, US, Public Library of Science (PLoS) (2014), Nr. 9'
havard: P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe,
C. Knabbe, D. Hendig, Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts
Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls, PLOS ONE
/ Public Library of Science . 9 (2014).
ieee: 'P. Kuzaj et al., “Large-Scaled Metabolic Profiling of Human Dermal
Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls,”
PLOS ONE / Public Library of Science , vol. 9, no. 9, Art. no. e108336,
2014, doi: 10.1371/journal.pone.0108336.'
mla: Kuzaj, Patricia, et al. “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts
Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls.” PLOS
ONE / Public Library of Science , vol. 9, no. 9, e108336, 2014, https://doi.org/10.1371/journal.pone.0108336.
short: P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe,
C. Knabbe, D. Hendig, PLOS ONE / Public Library of Science 9 (2014).
ufg: 'Kuzaj, Patricia u. a.: Large-Scaled Metabolic Profiling of Human Dermal
Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls,
in: PLOS ONE / Public Library of Science 9 (2014), H. 9.'
van: Kuzaj P, Kuhn J, Michalek RD, Karoly ED, Faust I, Dabisch-Ruthe M, et al. Large-Scaled
Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
Patients and Healthy Controls. PLOS ONE / Public Library of Science . 2014;9(9).
date_created: 2025-06-17T06:54:30Z
date_updated: 2025-06-17T13:49:26Z
department:
- _id: DEP4010
doi: 10.1371/journal.pone.0108336
intvolume: ' 9'
issue: '9'
language:
- iso: eng
place: San Francisco, California, US
publication: 'PLOS ONE / Public Library of Science '
publication_identifier:
eissn:
- 1932-6203
publication_status: published
publisher: Public Library of Science (PLoS)
quality_controlled: '1'
status: public
title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma
Elasticum Patients and Healthy Controls
type: scientific_journal_article
user_id: '83781'
volume: 9
year: '2014'
...
---
_id: '5348'
author:
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
citation:
ama: 'Schwarzer K, Schneider J, Becker B, Müller U. Entkeimungsauslegung mit der
"Lemgo D- and z-value Database for Food. In: ; 2012.'
apa: Schwarzer, K., Schneider, J., Becker, B., & Müller, U. (2012). Entkeimungsauslegung
mit der "Lemgo D- and z-value Database for Food. ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik
, Stuttgart - Hohenheim.
bjps: Schwarzer K et al. (2012) Entkeimungsauslegung mit der "Lemgo
D- and z-value Database for Food.
chicago: Schwarzer, Knut, Jan Schneider, Barbara Becker, and Ulrich Müller. “Entkeimungsauslegung
mit der "Lemgo D- and z-value Database for Food,” 2012.
chicago-de: 'Schwarzer, Knut, Jan Schneider, Barbara Becker und Ulrich Müller. 2012.
Entkeimungsauslegung mit der "Lemgo D- and z-value Database for Food. In: .'
din1505-2-1: 'Schwarzer, Knut ; Schneider, Jan ; Becker,
Barbara ; Müller, Ulrich:
Entkeimungsauslegung mit der "Lemgo D- and z-value Database for Food. In: , 2012'
havard: 'K. Schwarzer, J. Schneider, B. Becker, U. Müller, Entkeimungsauslegung
mit der "Lemgo D- and z-value Database for Food, in: 2012.'
ieee: K. Schwarzer, J. Schneider, B. Becker, and U. Müller, “Entkeimungsauslegung
mit der "Lemgo D- and z-value Database for Food,” presented at the ProcessNet-Fachgruppe
Lebensmittelverfahrenstechnik , Stuttgart - Hohenheim, 2012.
mla: Schwarzer, Knut, et al. Entkeimungsauslegung mit der "Lemgo D- and z-value
Database for Food. 2012.
short: 'K. Schwarzer, J. Schneider, B. Becker, U. Müller, in: 2012.'
ufg: 'Schwarzer, Knut u. a.: Entkeimungsauslegung mit der "Lemgo D- and z-value
Database for Food, in: o. Hg.: o. O. 2012.'
van: Schwarzer K, Schneider J, Becker B, Müller U. Entkeimungsauslegung mit der
"Lemgo D- and z-value Database for Food. In 2012.
conference:
end_date: 2012-03-21
location: Stuttgart - Hohenheim
name: 'ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik '
start_date: 2012-03-19
date_created: 2021-03-30T07:24:31Z
date_updated: 2024-05-24T06:46:24Z
department:
- _id: DEP4009
- _id: DEP4023
- _id: DEP4010
- _id: DEP4018
language:
- iso: ger
status: public
title: Entkeimungsauslegung mit der "Lemgo D- and z-value Database for Food
type: conference
user_id: '83779'
year: '2012'
...
---
_id: '12983'
abstract:
- lang: eng
text: "Background\r\nA broad spectrum of pathogens is causative for respiratory
tract infections, but symptoms are mostly similar. Therefore, the identification
of the causative viruses and bacteria is only feasible using multiplex PCR or
several monoplex PCR tests in parallel.\r\nMethods\r\nThe analytical sensitivity
of three multiplex PCR assays, RespiFinder-19, RespiFinder-SMART-22 and xTAG-Respiratory-Virus-Panel-Fast-Assay
(RVP), were compared to monoplex real-time PCR with quantified standardized control
material. All assays include the most common respiratory pathogens.\r\nResults\r\nTo
compare the analytical sensitivity of the multiplex assays, samples were inoculated
with 13 different quantified viruses in the range of 101 to 105 copies/ml. Concordant
results were received for rhinovirus, whereas the RVP detected influenzavirus,
RSV and hMPV more frequently in low concentrations. The RespiFinder-19 and the
RespiFinder-SMART-22 showed a higher analytical sensitivity for adenoviruses and
coronaviruses, whereas the RVP was incapable to detect adenovirus and coronavirus
in concentrations of 104 copies/ml. The RespiFinder-19 and RespiFinder-SMART-22A
did not detect influenzaviruses (104 copies/ml) and RSV (103 copies/ml). The detection
of all 13 viruses in one sample was only achieved using monoplex PCR. To analyze
possible competitive amplification reactions between the different viruses, samples
were further inoculated with only 4 different viruses in one sample. Compared
to the detection of 13 viruses in parallel, only a few differences were found.\r\nThe
incidence of respiratory viruses was compared in tracheal secretion (TS) samples
(n = 100) of mechanically ventilated patients in winter (n = 50) and summer (n = 50).
In winter, respiratory viruses were detected in 32 TS samples (64%) by RespiFinder-19,
whereas the detection rate with RVP was only 22%. The most frequent viruses were
adenovirus (32%) and PIV-2 (20%). Multiple infections were detected in 16 TS samples
(32%) by RespiFinder-19. Fewer infections were found in summer (RespiFinder-19:
20%; RVP: 6%). All positive results were verified using monoplex PCR.\r\nConclusions\r\nMultiplex
PCR tests have a broad spectrum of pathogens to test at a time. Analysis of multiple
inoculated samples revealed a different focus of the detected virus types by the
three assays. Analysis of clinical samples showed a high concordance of detected
viruses by the RespiFinder-19 compared to monoplex tests."
article_number: '163'
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Tanja
full_name: Vollmer, Tanja
last_name: Vollmer
- first_name: Ortwin
full_name: Adams, Ortwin
last_name: Adams
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Jens
full_name: Dreier, Jens
last_name: Dreier
citation:
ama: 'Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. BMC Infectious Diseases. 2012;12(1). doi:10.1186/1471-2334-12-163'
apa: 'Dabisch-Ruthe, M., Vollmer, T., Adams, O., Knabbe, C., & Dreier, J. (2012).
Comparison of three multiplex PCR assays for the detection of respiratory viral
infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder
19 assay and RespiFinder SMART 22 assay. BMC Infectious Diseases, 12(1),
Article 163. https://doi.org/10.1186/1471-2334-12-163'
bjps: 'Dabisch-Ruthe M et al. (2012) Comparison of Three Multiplex
PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG
Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART
22 Assay. BMC Infectious Diseases 12.'
chicago: 'Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe,
and Jens Dreier. “Comparison of Three Multiplex PCR Assays for the Detection of
Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast
Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” BMC Infectious
Diseases 12, no. 1 (2012). https://doi.org/10.1186/1471-2334-12-163.'
chicago-de: 'Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe
und Jens Dreier. 2012. Comparison of three multiplex PCR assays for the detection
of respiratory viral infections: evaluation of xTAG respiratory virus panel fast
assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infectious
Diseases 12, Nr. 1. doi:10.1186/1471-2334-12-163,
.'
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Vollmer, Tanja ; Adams,
Ortwin ; Knabbe, Cornelius
; Dreier, Jens: Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. In: BMC Infectious Diseases Bd. 12. Berlin ; Heidelberg,
Springer (2012), Nr. 1'
havard: 'M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, Comparison
of three multiplex PCR assays for the detection of respiratory viral infections:
evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and
RespiFinder SMART 22 assay, BMC Infectious Diseases. 12 (2012).'
ieee: 'M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, and J. Dreier, “Comparison
of three multiplex PCR assays for the detection of respiratory viral infections:
evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and
RespiFinder SMART 22 assay,” BMC Infectious Diseases, vol. 12, no. 1, Art.
no. 163, 2012, doi: 10.1186/1471-2334-12-163.'
mla: 'Dabisch-Ruthe, Mareike, et al. “Comparison of Three Multiplex PCR Assays for
the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory
Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.”
BMC Infectious Diseases, vol. 12, no. 1, 163, 2012, https://doi.org/10.1186/1471-2334-12-163.'
short: M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, BMC Infectious
Diseases 12 (2012).
ufg: 'Dabisch-Ruthe, Mareike u. a.: Comparison of three multiplex PCR assays
for the detection of respiratory viral infections: evaluation of xTAG respiratory
virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, in:
BMC Infectious Diseases 12 (2012), H. 1.'
van: 'Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. BMC Infectious Diseases. 2012;12(1).'
date_created: 2025-06-17T06:52:13Z
date_updated: 2025-06-17T13:49:03Z
department:
- _id: DEP4010
doi: 10.1186/1471-2334-12-163
intvolume: ' 12'
issue: '1'
keyword:
- Respiratory Virus
- Elution Volume
- Multiplex Assay
- Multiple Infection
- Bocavirus
language:
- iso: eng
place: Berlin ; Heidelberg
publication: BMC Infectious Diseases
publication_identifier:
eissn:
- 1471-2334
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: 'Comparison of three multiplex PCR assays for the detection of respiratory
viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder
19 assay and RespiFinder SMART 22 assay'
type: scientific_journal_article
user_id: '83781'
volume: 12
year: '2012'
...
---
_id: '2748'
author:
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Patrick
full_name: Wilhelm, Patrick
id: '12013'
last_name: Wilhelm
citation:
ama: 'Schneider J, Müller U, Schwarzer K, Becker B, Wilhelm P. Lemgo Database for
D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International.
2010;(5):252-256.'
apa: 'Schneider, J., Müller, U., Schwarzer, K., Becker, B., & Wilhelm, P. (2010).
Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim.
Brauwelt International, 5, 252–256.'
bjps: 'Schneider J et al. (2010) Lemgo Database for D- and z-Values:
Useful for Beverage Spoilage Microorgansim. Brauwelt International 252–256.'
chicago: 'Schneider, Jan, Ulrich Müller, Knut Schwarzer, Barbara Becker, and Patrick
Wilhelm. “Lemgo Database for D- and z-Values: Useful for Beverage Spoilage Microorgansim.”
Brauwelt International, no. 5 (2010): 252–56.'
chicago-de: 'Schneider, Jan, Ulrich Müller, Knut Schwarzer, Barbara Becker und Patrick
Wilhelm. 2010. Lemgo Database for D- and z-values: useful for beverage spoilage
microorgansim. Brauwelt International, Nr. 5: 252–256.'
din1505-2-1: 'Schneider, Jan ; Müller, Ulrich ; Schwarzer,
Knut ; Becker, Barbara ;
Wilhelm, Patrick: Lemgo Database
for D- and z-values: useful for beverage spoilage microorgansim. In: Brauwelt
International, Fachverlag Hans Carl GmbH (2010), Nr. 5, S. 252–256'
havard: 'J. Schneider, U. Müller, K. Schwarzer, B. Becker, P. Wilhelm, Lemgo Database
for D- and z-values: useful for beverage spoilage microorgansim, Brauwelt International.
(2010) 252–256.'
ieee: 'J. Schneider, U. Müller, K. Schwarzer, B. Becker, and P. Wilhelm, “Lemgo
Database for D- and z-values: useful for beverage spoilage microorgansim,” Brauwelt
International, no. 5, pp. 252–256, 2010.'
mla: 'Schneider, Jan, et al. “Lemgo Database for D- and z-Values: Useful for Beverage
Spoilage Microorgansim.” Brauwelt International, no. 5, 2010, pp. 252–56.'
short: J. Schneider, U. Müller, K. Schwarzer, B. Becker, P. Wilhelm, Brauwelt International
(2010) 252–256.
ufg: 'Schneider, Jan u. a.: Lemgo Database for D- and z-values: useful for
beverage spoilage microorgansim, in: Brauwelt International (2010), H.
5, S. 252–256.'
van: 'Schneider J, Müller U, Schwarzer K, Becker B, Wilhelm P. Lemgo Database for
D- and z-values: useful for beverage spoilage microorgansim. Brauwelt International.
2010;(5):252–6.'
date_created: 2020-06-02T10:24:20Z
date_updated: 2023-03-15T13:49:42Z
department:
- _id: DEP4010
- _id: DEP4009
- _id: DEP4018
issue: '5'
language:
- iso: eng
page: 252 - 256
publication: Brauwelt International
publication_identifier:
issn:
- 0934-9340
publication_status: published
publisher: Fachverlag Hans Carl GmbH
status: public
title: 'Lemgo Database for D- and z-values: useful for beverage spoilage microorgansim'
type: journal_article
user_id: '12013'
year: 2010
...
---
_id: '2763'
author:
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Patrick
full_name: Wilhelm, Patrick
id: '12013'
last_name: Wilhelm
citation:
ama: 'Schwarzer K, Schneider J, Müller U, Becker B, Wilhelm P. Lemgoer Datenbank
für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt.
2010;150(18):540-544.'
apa: 'Schwarzer, K., Schneider, J., Müller, U., Becker, B., & Wilhelm, P. (2010).
Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen.
Brauwelt, 150(18), 540–544.'
bjps: 'Schwarzer K et al. (2010) Lemgoer Datenbank Für D- Und z-Werte:
Anwendung Für Getränkeschädliche Mikroorganismen. Brauwelt 150,
540–544.'
chicago: 'Schwarzer, Knut, Jan Schneider, Ulrich Müller, Barbara Becker, and Patrick
Wilhelm. “Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für Getränkeschädliche
Mikroorganismen.” Brauwelt 150, no. 18 (2010): 540–44.'
chicago-de: 'Schwarzer, Knut, Jan Schneider, Ulrich Müller, Barbara Becker und Patrick
Wilhelm. 2010. Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche
Mikroorganismen. Brauwelt 150, Nr. 18: 540–544.'
din1505-2-1: 'Schwarzer, Knut ; Schneider, Jan ; Müller,
Ulrich ; Becker, Barbara
; Wilhelm, Patrick: Lemgoer Datenbank
für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. In: Brauwelt
Bd. 150, Fachverlag Hans Carl GmbH (2010), Nr. 18, S. 540–544'
havard: 'K. Schwarzer, J. Schneider, U. Müller, B. Becker, P. Wilhelm, Lemgoer Datenbank
für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen, Brauwelt.
150 (2010) 540–544.'
ieee: 'K. Schwarzer, J. Schneider, U. Müller, B. Becker, and P. Wilhelm, “Lemgoer
Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen,”
Brauwelt, vol. 150, no. 18, pp. 540–544, 2010.'
mla: 'Schwarzer, Knut, et al. “Lemgoer Datenbank Für D- Und z-Werte: Anwendung Für
Getränkeschädliche Mikroorganismen.” Brauwelt, vol. 150, no. 18, 2010,
pp. 540–44.'
short: K. Schwarzer, J. Schneider, U. Müller, B. Becker, P. Wilhelm, Brauwelt 150
(2010) 540–544.
ufg: 'Schwarzer, Knut u. a.: Lemgoer Datenbank für D- und z-Werte: Anwendung
für getränkeschädliche Mikroorganismen, in: Brauwelt 150 (2010), H. 18,
S. 540–544.'
van: 'Schwarzer K, Schneider J, Müller U, Becker B, Wilhelm P. Lemgoer Datenbank
für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen. Brauwelt.
2010;150(18):540–4.'
date_created: 2020-06-03T07:13:41Z
date_updated: 2023-03-15T13:49:42Z
department:
- _id: DEP4010
- _id: DEP4009
- _id: DEP4018
intvolume: ' 150'
issue: '18'
language:
- iso: eng
page: 540 - 544
publication: Brauwelt
publication_identifier:
issn:
- "\t0724-696X"
publication_status: published
publisher: Fachverlag Hans Carl GmbH
status: public
title: 'Lemgoer Datenbank für D- und z-Werte: Anwendung für getränkeschädliche Mikroorganismen'
type: journal_article
user_id: '12013'
volume: 150
year: 2010
...
---
_id: '2361'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Goetz
full_name: Hildebrandt, Goetz
last_name: Hildebrandt
- first_name: 'Irfan '
full_name: 'Erol, Irfan '
last_name: Erol
- first_name: Josef
full_name: Kleer, Josef
last_name: Kleer
- first_name: Aranya Sira
full_name: Manopas, Aranya Sira
last_name: Manopas
citation:
ama: Becker B, Hildebrandt G, Erol I, Kleer J, Manopas AS. Von der Döner - Prüfung
zum Döner - Heariing. Fleischwirtschaft . 2009:61-65.
apa: Becker, B., Hildebrandt, G., Erol, I., Kleer, J., & Manopas, A. S. (2009).
Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft , pp. 61–65.
bjps: Becker B et al. (2009) Von Der Döner - Prüfung Zum Döner - Heariing.
Fleischwirtschaft , 61–65.
chicago: Becker, Barbara, Goetz Hildebrandt, Irfan Erol, Josef Kleer, and Aranya
Sira Manopas. “Von Der Döner - Prüfung Zum Döner - Heariing.” Fleischwirtschaft
, 2009.
chicago-de: Becker, Barbara, Goetz Hildebrandt, Irfan Erol, Josef Kleer und Aranya
Sira Manopas. 2009. Von der Döner - Prüfung zum Döner - Heariing. Fleischwirtschaft
.
din1505-2-1: 'Becker, Barbara ; Hildebrandt, Goetz ; Erol,
Irfan ; Kleer, Josef ; Manopas, Aranya Sira: Von der Döner -
Prüfung zum Döner - Heariing. In: Fleischwirtschaft (2009), Nr. 7'
havard: B. Becker, G. Hildebrandt, I. Erol, J. Kleer, A.S. Manopas, Von der Döner
- Prüfung zum Döner - Heariing, Fleischwirtschaft . (2009) 61–65.
ieee: B. Becker, G. Hildebrandt, I. Erol, J. Kleer, and A. S. Manopas, “Von der
Döner - Prüfung zum Döner - Heariing,” Fleischwirtschaft , no. 7, pp. 61–65,
2009.
mla: Becker, Barbara, et al. “Von Der Döner - Prüfung Zum Döner - Heariing.” Fleischwirtschaft
, no. 7, 2009, pp. 61–65.
short: B. Becker, G. Hildebrandt, I. Erol, J. Kleer, A.S. Manopas, Fleischwirtschaft (2009)
61–65.
ufg: 'Becker, Barbara et. al. (2009): Von der Döner - Prüfung zum Döner -
Heariing, in: Fleischwirtschaft (7) (2009), S. 61–65.'
van: Becker B, Hildebrandt G, Erol I, Kleer J, Manopas AS. Von der Döner - Prüfung
zum Döner - Heariing. Fleischwirtschaft . 2009;61–5.
date_created: 2020-05-18T09:40:45Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4010
issue: '7'
language:
- iso: eng
page: 61-65
publication: 'Fleischwirtschaft '
publication_date: 19.03.2019
status: public
title: Von der Döner - Prüfung zum Döner - Heariing
type: newspaper_article
user_id: '74222'
year: 2009
...
---
_id: '6128'
author:
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
citation:
ama: 'Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase
for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der
Entkeimung. In: ; 2009.'
apa: 'Schwarzer, K., Becker, B., Schneider, J., & Müller, U. (2009). Die
Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. ProcessNet Fachausschuss Lebensmittelverfahrenstechnik,
Lausanne.'
bjps: 'Schwarzer K et al. (2009) Die Lemgo D- and z-Value Datebase
for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der
Entkeimung.'
chicago: 'Schwarzer, Knut, Barbara Becker, Jan Schneider, and Ulrich Müller. “Die
Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung
von Minimal Processing in Der Entkeimung,” 2009.'
chicago-de: 'Schwarzer, Knut, Barbara Becker, Jan Schneider und Ulrich Müller. 2009.
Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. In: .'
din1505-2-1: 'Schwarzer, Knut ; Becker, Barbara ; Schneider,
Jan ; Müller, Ulrich: Die
Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. In: , 2009'
havard: 'K. Schwarzer, B. Becker, J. Schneider, U. Müller, Die Lemgo D- and z-value
Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing
in der Entkeimung, in: 2009.'
ieee: 'K. Schwarzer, B. Becker, J. Schneider, and U. Müller, “Die Lemgo D- and z-value
Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing
in der Entkeimung,” presented at the ProcessNet Fachausschuss Lebensmittelverfahrenstechnik,
Lausanne, 2009.'
mla: 'Schwarzer, Knut, et al. Die Lemgo D- and z-Value Datebase for Food (LDzBase):
Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung. 2009.'
short: 'K. Schwarzer, B. Becker, J. Schneider, U. Müller, in: 2009.'
ufg: 'Schwarzer, Knut u. a.: Die Lemgo D- and z-value Datebase for Food (LDzBase):
Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: o. Hg.:
o. O. 2009.'
van: 'Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase
for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der
Entkeimung. In 2009.'
conference:
end_date: 2009-03-25
location: Lausanne
name: ProcessNet Fachausschuss Lebensmittelverfahrenstechnik
start_date: 2009-03-23
date_created: 2021-09-06T10:43:56Z
date_updated: 2024-05-23T11:12:12Z
department:
- _id: DEP4023
- _id: DEP4009
- _id: DEP4010
- _id: DEP4018
language:
- iso: eng
status: public
title: 'Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung'
type: conference_abstract
user_id: '83779'
year: '2009'
...
---
_id: '6141'
author:
- first_name: Knut
full_name: Schwarzer, Knut
id: '13329'
last_name: Schwarzer
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
- first_name: Jan
full_name: Schneider, Jan
id: '13209'
last_name: Schneider
orcid: 0000-0001-6401-8873
- first_name: Ulrich
full_name: Müller, Ulrich
id: '12119'
last_name: Müller
citation:
ama: 'Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase
for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der
Entkeimung. In: ; 2009.'
apa: 'Schwarzer, K., Becker, B., Schneider, J., & Müller, U. (2009). Die
Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. GDL-Kongress , Lemgo.'
bjps: 'Schwarzer K et al. (2009) Die Lemgo D- and z-Value Datebase
for Food (LDzBase): Ein Werkzeug Zur Einführung von Minimal Processing in Der
Entkeimung.'
chicago: 'Schwarzer, Knut, Barbara Becker, Jan Schneider, and Ulrich Müller. “Die
Lemgo D- and z-Value Datebase for Food (LDzBase): Ein Werkzeug Zur Einführung
von Minimal Processing in Der Entkeimung,” 2009.'
chicago-de: 'Schwarzer, Knut, Barbara Becker, Jan Schneider und Ulrich Müller. 2009.
Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. In: .'
din1505-2-1: 'Schwarzer, Knut ; Becker, Barbara ; Schneider,
Jan ; Müller, Ulrich: Die
Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung. In: , 2009'
havard: 'K. Schwarzer, B. Becker, J. Schneider, U. Müller, Die Lemgo D- and z-value
Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing
in der Entkeimung, in: 2009.'
ieee: 'K. Schwarzer, B. Becker, J. Schneider, and U. Müller, “Die Lemgo D- and z-value
Datebase for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing
in der Entkeimung,” presented at the GDL-Kongress , Lemgo, 2009.'
mla: 'Schwarzer, Knut, et al. Die Lemgo D- and z-Value Datebase for Food (LDzBase):
Ein Werkzeug Zur Einführung von Minimal Processing in Der Entkeimung. 2009.'
short: 'K. Schwarzer, B. Becker, J. Schneider, U. Müller, in: 2009.'
ufg: 'Schwarzer, Knut u. a.: Die Lemgo D- and z-value Datebase for Food (LDzBase):
Ein Werkzeug zur Einführung von minimal processing in der Entkeimung, in: o. Hg.:
o. O. 2009.'
van: 'Schwarzer K, Becker B, Schneider J, Müller U. Die Lemgo D- and z-value Datebase
for Food (LDzBase): Ein Werkzeug zur Einführung von minimal processing in der
Entkeimung. In 2009.'
conference:
end_date: 2009-10-24
location: Lemgo
name: 'GDL-Kongress '
start_date: 2009-10-22
date_created: 2021-09-06T11:10:55Z
date_updated: 2024-05-23T11:12:44Z
department:
- _id: DEP4023
- _id: DEP4009
- _id: DEP4010
- _id: DEP4018
language:
- iso: eng
status: public
title: 'Die Lemgo D- and z-value Datebase for Food (LDzBase): Ein Werkzeug zur Einführung
von minimal processing in der Entkeimung'
type: conference_abstract
user_id: '83779'
year: '2009'
...
---
_id: '12982'
abstract:
- lang: eng
text: Contaminated food is a significant vehicle for human norovirus transmission.
The present study determined the effect of physicochemical treatments on the tenacity
of infective human norovirus genogroup II in selected foods. Artificially contaminated
produce was subjected to a number of processes used by the food industry for preservation
and by the consumer for storage and preparation. Virus recovery was carried out
by using ultrafiltration and was monitored by using bacteriophage MS2 as an internal
process control. Norovirus was quantified by using monoplex one-step TaqMan real-time
reverse transcription (RT)-PCR and an external standard curve based on recombinant
RNA standards. An RNase pretreatment step was used to avoid false-positive PCR
results caused by accessible RNA, which allowed detection of intact virus particles.
Significant reductions in titers were obtained with heat treatments usually applied
by consumers for food preparation (baking, cooking, roasting). Generally, processes
used for preservation and storage, such as cooling, freezing, acidification (≥pH
4.5), and moderate heat treatments (pasteurization), appear to be insufficient
to inactivate norovirus within a food matrix or on the surface of food. Besides
data for persistence in processed food, comparable data for individual matrix-specific
protective effects, recovery rates, and inhibitory effects on the PCRs were obtained
in this study. The established procedure might be used for other noncultivable
enteric RNA viruses that are connected to food-borne diseases. The data obtained
in this study may also help optimize the process for inactivation of norovirus
in food by adjusting food processing technologies and may promote the development
of risk assessment systems in order to improve consumer protection.
author:
- first_name: Sascha
full_name: Mormann, Sascha
last_name: Mormann
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
citation:
ama: Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on
the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
Foods. Applied and environmental microbiology / American Society for Microbiology.
2009;76(2):536-545. doi:10.1128/aem.01797-09
apa: Mormann, S., Dabisch-Ruthe, M., & Becker, B. (2009). Effects of Technological
Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally
Contaminated Foods. Applied and Environmental Microbiology / American Society
for Microbiology, 76(2), 536–545. https://doi.org/10.1128/aem.01797-09
bjps: Mormann S, Dabisch-Ruthe M and Becker B (2009) Effects of Technological
Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally
Contaminated Foods. Applied and environmental microbiology / American Society
for Microbiology 76, 536–545.
chicago: 'Mormann, Sascha, Mareike Dabisch-Ruthe, and Barbara Becker. “Effects of
Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
II in Experimentally Contaminated Foods.” Applied and Environmental Microbiology
/ American Society for Microbiology 76, no. 2 (2009): 536–45. https://doi.org/10.1128/aem.01797-09.'
chicago-de: 'Mormann, Sascha, Mareike Dabisch-Ruthe und Barbara Becker. 2009. Effects
of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
II in Experimentally Contaminated Foods. Applied and environmental microbiology
/ American Society for Microbiology 76, Nr. 2: 536–545. doi:10.1128/aem.01797-09,
.'
din1505-2-1: 'Mormann, Sascha ; Dabisch-Ruthe, Mareike ; Becker,
Barbara: Effects of Technological Processes on the Tenacity and Inactivation
of Norovirus Genogroup II in Experimentally Contaminated Foods. In: Applied
and environmental microbiology / American Society for Microbiology Bd. 76.
Washington, DC [u.a.], American Society for Microbiology (2009), Nr. 2, S. 536–545'
havard: S. Mormann, M. Dabisch-Ruthe, B. Becker, Effects of Technological Processes
on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
Foods, Applied and Environmental Microbiology / American Society for Microbiology.
76 (2009) 536–545.
ieee: 'S. Mormann, M. Dabisch-Ruthe, and B. Becker, “Effects of Technological Processes
on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
Foods,” Applied and environmental microbiology / American Society for Microbiology,
vol. 76, no. 2, pp. 536–545, 2009, doi: 10.1128/aem.01797-09.'
mla: Mormann, Sascha, et al. “Effects of Technological Processes on the Tenacity
and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods.”
Applied and Environmental Microbiology / American Society for Microbiology,
vol. 76, no. 2, 2009, pp. 536–45, https://doi.org/10.1128/aem.01797-09.
short: S. Mormann, M. Dabisch-Ruthe, B. Becker, Applied and Environmental Microbiology
/ American Society for Microbiology 76 (2009) 536–545.
ufg: 'Mormann, Sascha/Dabisch-Ruthe, Mareike/Becker, Barbara: Effects of
Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
II in Experimentally Contaminated Foods, in: Applied and environmental microbiology
/ American Society for Microbiology 76 (2009), H. 2, S. 536–545.'
van: Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on
the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
Foods. Applied and environmental microbiology / American Society for Microbiology.
2009;76(2):536–45.
date_created: 2025-06-17T06:26:59Z
date_updated: 2025-06-17T13:49:35Z
department:
- _id: DEP4010
doi: 10.1128/aem.01797-09
intvolume: ' 76'
issue: '2'
language:
- iso: eng
page: 536-545
place: Washington, DC [u.a.]
publication: ' Applied and environmental microbiology / American Society for Microbiology'
publication_identifier:
eissn:
- 1098-5336
issn:
- '0099-2240 '
publication_status: published
publisher: American Society for Microbiology
quality_controlled: '1'
status: public
title: Effects of Technological Processes on the Tenacity and Inactivation of Norovirus
Genogroup II in Experimentally Contaminated Foods
type: scientific_journal_article
user_id: '83781'
volume: 76
year: '2009'
...
---
_id: '2359'
author:
- first_name: Barbara
full_name: Becker, Barbara
id: '12640'
last_name: Becker
citation:
ama: 'Becker B. Lebensmittelassoziierte Viren - aktuelle Apekte. In: ; 2004.'
apa: Becker, B. (2004). Lebensmittelassoziierte Viren - aktuelle Apekte. Presented
at the Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie.
bjps: Becker B (2004) Lebensmittelassoziierte Viren - Aktuelle Apekte.
chicago: Becker, Barbara. “Lebensmittelassoziierte Viren - Aktuelle Apekte,” 2004.
chicago-de: 'Becker, Barbara. 2004. Lebensmittelassoziierte Viren - aktuelle Apekte.
In: .'
din1505-2-1: 'Becker, Barbara: Lebensmittelassoziierte
Viren - aktuelle Apekte. In: , 2004'
havard: 'B. Becker, Lebensmittelassoziierte Viren - aktuelle Apekte, in: 2004.'
ieee: B. Becker, “Lebensmittelassoziierte Viren - aktuelle Apekte,” presented at
the Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie, 2004.
mla: Becker, Barbara. Lebensmittelassoziierte Viren - Aktuelle Apekte. 2004.
short: 'B. Becker, in: 2004.'
ufg: 'Becker, Barbara (2004): Lebensmittelassoziierte Viren - aktuelle Apekte,
in: .'
van: Becker B. Lebensmittelassoziierte Viren - aktuelle Apekte. In 2004.
conference:
end_date: 16.07.2004
name: Schnellmethoden und Automatisierung in der Lebensmitel Mikrobiologie
start_date: 14.07.2004
date_created: 2020-05-18T09:33:00Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4010
language:
- iso: eng
status: public
title: Lebensmittelassoziierte Viren - aktuelle Apekte
type: conference
user_id: '74222'
year: 2004
...