---
_id: '13315'
abstract:
- lang: eng
  text: Determining cell density and viability with (semi‐)automated methods enables
    rapid cell cultivation analysis. However, scientific validation is required, typically
    by comparing results to manual counting. To address this, we present a method
    to evaluate and compare two cell counting methods exemplified by a manual and
    a semi‐automated method (Countstar BioTech). Experiments followed validation parameters
    aligned with the International Council for Harmonization (ICH) Q2(R1) guideline
    and a dilution series design based on ISO 20391‐2:2019. Both the semi‐automated
    and manual methods showed comparable specificity and linearity for Chinese hamster
    ovary (CHO)‐K1 cells. The semi‐automated method exhibited superior repeatability
    for total cell density, whereas cell viability results showed no significant difference.
author:
- first_name: Selina
  full_name: Uhlendorff, Selina
  id: '68713'
  last_name: Uhlendorff
  orcid: https://orcid.org/0000-0002-0502-8032
- first_name: Ulrich
  full_name: Odefey, Ulrich
  id: '74218'
  last_name: Odefey
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
- first_name: Miriam
  full_name: Pein-Hackelbusch, Miriam
  id: '64952'
  last_name: Pein-Hackelbusch
  orcid: 0000-0002-7920-0595
citation:
  ama: Uhlendorff S, Odefey U, Frahm B, Pein-Hackelbusch M. Performance Comparison
    between Semi‐Automated and Manual Cell Counting for Animal Cell Culture. <i>Chemie
    Ingenieur Technik</i>. 2025;98(1-2):57-68. doi:<a href="https://doi.org/10.1002/cite.70048">10.1002/cite.70048</a>
  apa: Uhlendorff, S., Odefey, U., Frahm, B., &#38; Pein-Hackelbusch, M. (2025). Performance
    Comparison between Semi‐Automated and Manual Cell Counting for Animal Cell Culture.
    <i>Chemie Ingenieur Technik</i>, <i>98</i>(1–2), 57–68. <a href="https://doi.org/10.1002/cite.70048">https://doi.org/10.1002/cite.70048</a>
  bjps: <b>Uhlendorff S <i>et al.</i></b> (2025) Performance Comparison between Semi‐Automated
    and Manual Cell Counting for Animal Cell Culture. <i>Chemie Ingenieur Technik</i>
    <b>98</b>, 57–68.
  chicago: 'Uhlendorff, Selina, Ulrich Odefey, Björn Frahm, and Miriam Pein-Hackelbusch.
    “Performance Comparison between Semi‐Automated and Manual Cell Counting for Animal
    Cell Culture.” <i>Chemie Ingenieur Technik</i> 98, no. 1–2 (2025): 57–68. <a href="https://doi.org/10.1002/cite.70048">https://doi.org/10.1002/cite.70048</a>.'
  chicago-de: 'Uhlendorff, Selina, Ulrich Odefey, Björn Frahm und Miriam Pein-Hackelbusch.
    2025. Performance Comparison between Semi‐Automated and Manual Cell Counting for
    Animal Cell Culture. <i>Chemie Ingenieur Technik</i> 98, Nr. 1–2: 57–68. doi:<a
    href="https://doi.org/10.1002/cite.70048">10.1002/cite.70048</a>, .'
  din1505-2-1: '<span style="font-variant:small-caps;">Uhlendorff, Selina</span> ;
    <span style="font-variant:small-caps;">Odefey, Ulrich</span> ; <span style="font-variant:small-caps;">Frahm,
    Björn</span> ; <span style="font-variant:small-caps;">Pein-Hackelbusch, Miriam</span>:
    Performance Comparison between Semi‐Automated and Manual Cell Counting for Animal
    Cell Culture. In: <i>Chemie Ingenieur Technik</i> Bd. 98. Weinheim, Wiley (2025),
    Nr. 1–2, S. 57–68'
  havard: S. Uhlendorff, U. Odefey, B. Frahm, M. Pein-Hackelbusch, Performance Comparison
    between Semi‐Automated and Manual Cell Counting for Animal Cell Culture, Chemie
    Ingenieur Technik. 98 (2025) 57–68.
  ieee: 'S. Uhlendorff, U. Odefey, B. Frahm, and M. Pein-Hackelbusch, “Performance
    Comparison between Semi‐Automated and Manual Cell Counting for Animal Cell Culture,”
    <i>Chemie Ingenieur Technik</i>, vol. 98, no. 1–2, pp. 57–68, 2025, doi: <a href="https://doi.org/10.1002/cite.70048">10.1002/cite.70048</a>.'
  mla: Uhlendorff, Selina, et al. “Performance Comparison between Semi‐Automated and
    Manual Cell Counting for Animal Cell Culture.” <i>Chemie Ingenieur Technik</i>,
    vol. 98, no. 1–2, 2025, pp. 57–68, <a href="https://doi.org/10.1002/cite.70048">https://doi.org/10.1002/cite.70048</a>.
  short: S. Uhlendorff, U. Odefey, B. Frahm, M. Pein-Hackelbusch, Chemie Ingenieur
    Technik 98 (2025) 57–68.
  ufg: '<b>Uhlendorff, Selina u. a.</b>: Performance Comparison between Semi‐Automated
    and Manual Cell Counting for Animal Cell Culture, in: <i>Chemie Ingenieur Technik</i>
    98 (2025), H. 1–2,  S. 57–68.'
  van: Uhlendorff S, Odefey U, Frahm B, Pein-Hackelbusch M. Performance Comparison
    between Semi‐Automated and Manual Cell Counting for Animal Cell Culture. Chemie
    Ingenieur Technik. 2025;98(1–2):57–68.
date_created: 2025-12-08T07:26:52Z
date_updated: 2026-03-27T17:14:42Z
department:
- _id: DEP4029
- _id: DEP4028
doi: 10.1002/cite.70048
intvolume: '        98'
issue: 1-2
keyword:
- Cell density
- Cell enumeration
- Cell viability
- CHO-K1
language:
- iso: eng
main_file_link:
- open_access: '1'
oa: '1'
page: 57–68
place: Weinheim
publication: Chemie Ingenieur Technik
publication_identifier:
  eissn:
  - 1522-2640
  issn:
  - 0009-286X
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: Performance Comparison between Semi‐Automated and Manual Cell Counting for
  Animal Cell Culture
type: scientific_journal_article
user_id: '45666'
volume: 98
year: '2025'
...
---
_id: '12402'
abstract:
- lang: eng
  text: In anaerobic technology, pH values are crucial for targeted volatile fatty
    acid production. While pH dynamics can be modeled using the Anaerobic Digestion
    Model No. 1 (ADM1), simulation results may be biased. To address this issue, the
    pH prediction routine of Visual Water, a specialized water chemistry simulator,
    was validated. Unlike ADM1, it accounts for ionic strength and activities while
    also providing an automated uncertainty analysis. The analysis revealed Visual Water
    simulations to better fit measured pH data from acidic solutions in a miniaturized
    stirred-tank reactor.
author:
- first_name: Pascal
  full_name: Kosse, Pascal
  last_name: Kosse
- first_name: Tanja
  full_name: Hernández Rodriguez, Tanja
  id: '52466'
  last_name: Hernández Rodriguez
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
- first_name: Manfred
  full_name: Lübken, Manfred
  last_name: Lübken
- first_name: Marc
  full_name: Wichern, Marc
  last_name: Wichern
citation:
  ama: Kosse P, Hernández Rodriguez T, Frahm B, Lübken M, Wichern M. Comparative Analysis
    of pH Prediction Routines in ADM1 and a Specialized Water Chemistry Simulator.
    <i>Chemie Ingenieur Technik</i>. 2024;96(4):528-534. doi:<a href="https://doi.org/10.1002/cite.202300188">10.1002/cite.202300188</a>
  apa: Kosse, P., Hernández Rodriguez, T., Frahm, B., Lübken, M., &#38; Wichern, M.
    (2024). Comparative Analysis of pH Prediction Routines in ADM1 and a Specialized
    Water Chemistry Simulator. <i>Chemie Ingenieur Technik</i>, <i>96</i>(4), 528–534.
    <a href="https://doi.org/10.1002/cite.202300188">https://doi.org/10.1002/cite.202300188</a>
  bjps: <b>Kosse P <i>et al.</i></b> (2024) Comparative Analysis of PH Prediction
    Routines in ADM1 and a Specialized Water Chemistry Simulator. <i>Chemie Ingenieur
    Technik</i> <b>96</b>, 528–534.
  chicago: 'Kosse, Pascal, Tanja Hernández Rodriguez, Björn Frahm, Manfred Lübken,
    and Marc Wichern. “Comparative Analysis of PH Prediction Routines in ADM1 and
    a Specialized Water Chemistry Simulator.” <i>Chemie Ingenieur Technik</i> 96,
    no. 4 (2024): 528–34. <a href="https://doi.org/10.1002/cite.202300188">https://doi.org/10.1002/cite.202300188</a>.'
  chicago-de: 'Kosse, Pascal, Tanja Hernández Rodriguez, Björn Frahm, Manfred Lübken
    und Marc Wichern. 2024. Comparative Analysis of pH Prediction Routines in ADM1
    and a Specialized Water Chemistry Simulator. <i>Chemie Ingenieur Technik</i> 96,
    Nr. 4: 528–534. doi:<a href="https://doi.org/10.1002/cite.202300188">10.1002/cite.202300188</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Kosse, Pascal</span> ; <span
    style="font-variant:small-caps;">Hernández Rodriguez, Tanja</span> ; <span style="font-variant:small-caps;">Frahm,
    Björn</span> ; <span style="font-variant:small-caps;">Lübken, Manfred</span> ;
    <span style="font-variant:small-caps;">Wichern, Marc</span>: Comparative Analysis
    of pH Prediction Routines in ADM1 and a Specialized Water Chemistry Simulator.
    In: <i>Chemie Ingenieur Technik</i> Bd. 96. Weinheim, Wiley (2024), Nr. 4, S. 528–534'
  havard: P. Kosse, T. Hernández Rodriguez, B. Frahm, M. Lübken, M. Wichern, Comparative
    Analysis of pH Prediction Routines in ADM1 and a Specialized Water Chemistry Simulator,
    Chemie Ingenieur Technik. 96 (2024) 528–534.
  ieee: 'P. Kosse, T. Hernández Rodriguez, B. Frahm, M. Lübken, and M. Wichern, “Comparative
    Analysis of pH Prediction Routines in ADM1 and a Specialized Water Chemistry Simulator,”
    <i>Chemie Ingenieur Technik</i>, vol. 96, no. 4, pp. 528–534, 2024, doi: <a href="https://doi.org/10.1002/cite.202300188">10.1002/cite.202300188</a>.'
  mla: Kosse, Pascal, et al. “Comparative Analysis of PH Prediction Routines in ADM1
    and a Specialized Water Chemistry Simulator.” <i>Chemie Ingenieur Technik</i>,
    vol. 96, no. 4, 2024, pp. 528–34, <a href="https://doi.org/10.1002/cite.202300188">https://doi.org/10.1002/cite.202300188</a>.
  short: P. Kosse, T. Hernández Rodriguez, B. Frahm, M. Lübken, M. Wichern, Chemie
    Ingenieur Technik 96 (2024) 528–534.
  ufg: '<b>Kosse, Pascal u. a.</b>: Comparative Analysis of pH Prediction Routines
    in ADM1 and a Specialized Water Chemistry Simulator, in: <i>Chemie Ingenieur Technik</i>
    96 (2024), H. 4,  S. 528–534.'
  van: Kosse P, Hernández Rodriguez T, Frahm B, Lübken M, Wichern M. Comparative Analysis
    of pH Prediction Routines in ADM1 and a Specialized Water Chemistry Simulator.
    Chemie Ingenieur Technik. 2024;96(4):528–34.
date_created: 2025-01-29T13:49:16Z
date_updated: 2025-06-25T12:42:09Z
department:
- _id: DEP4000
doi: 10.1002/cite.202300188
external_id:
  isi:
  - '001169606600001'
intvolume: '        96'
isi: '1'
issue: '4'
keyword:
- Anaerobic Digestion Model No. 1 (ADM1)
- Anaerobic technology
- pH simulation
- Uncertainty assessment
- Visual Water
language:
- iso: eng
page: 528-534
place: Weinheim
publication: Chemie Ingenieur Technik
publication_identifier:
  eissn:
  - 1522-2640
  issn:
  - 0009-286X
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: Comparative Analysis of pH Prediction Routines in ADM1 and a Specialized Water
  Chemistry Simulator
type: scientific_journal_article
user_id: '83781'
volume: 96
year: '2024'
...
---
_id: '11385'
abstract:
- lang: eng
  text: Accurate pH calculations are essential for scientists across different disciplines
    to design optimal reactor solutions, but they can be arduous to calculate for
    complex acid-base solutions. Visual Water is a powerful software tool that provides
    accurate pH calculations with automated mathematical uncertainty analysis. Its
    workflow is presented and validated using acids and bases, showing a deviation
    of < 0.2 pH units between measured and calculated pH values. This highlights the
    software's reliability, which can help to simplify the work of non-experts in
    water chemistry.
author:
- first_name: Pascal
  full_name: Kosse, Pascal
  last_name: Kosse
- first_name: Tanja
  full_name: Hernández Rodriguez, Tanja
  id: '52466'
  last_name: Hernández Rodriguez
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
- first_name: Manfred
  full_name: Lübken, Manfred
  last_name: Lübken
- first_name: Marc
  full_name: Wichern, Marc
  last_name: Wichern
citation:
  ama: Kosse P, Hernández Rodriguez T, Frahm B, Lübken M, Wichern M. Validation and
    Uncertainty Assessment of a Software‐Integrated Workflow for pH Calculations.
    <i>Chemie Ingenieur Technik</i>. 2023;95(12):1960-1969. doi:<a href="https://doi.org/10.1002/cite.202300082">10.1002/cite.202300082</a>
  apa: Kosse, P., Hernández Rodriguez, T., Frahm, B., Lübken, M., &#38; Wichern, M.
    (2023). Validation and Uncertainty Assessment of a Software‐Integrated Workflow
    for pH Calculations. <i>Chemie Ingenieur Technik</i>, <i>95</i>(12), 1960–1969.
    <a href="https://doi.org/10.1002/cite.202300082">https://doi.org/10.1002/cite.202300082</a>
  bjps: <b>Kosse P <i>et al.</i></b> (2023) Validation and Uncertainty Assessment
    of a Software‐Integrated Workflow for PH Calculations. <i>Chemie Ingenieur Technik</i>
    <b>95</b>, 1960–1969.
  chicago: 'Kosse, Pascal, Tanja Hernández Rodriguez, Björn Frahm, Manfred Lübken,
    and Marc Wichern. “Validation and Uncertainty Assessment of a Software‐Integrated
    Workflow for PH Calculations.” <i>Chemie Ingenieur Technik</i> 95, no. 12 (2023):
    1960–69. <a href="https://doi.org/10.1002/cite.202300082">https://doi.org/10.1002/cite.202300082</a>.'
  chicago-de: 'Kosse, Pascal, Tanja Hernández Rodriguez, Björn Frahm, Manfred Lübken
    und Marc Wichern. 2023. Validation and Uncertainty Assessment of a Software‐Integrated
    Workflow for pH Calculations. <i>Chemie Ingenieur Technik</i> 95, Nr. 12: 1960–1969.
    doi:<a href="https://doi.org/10.1002/cite.202300082">10.1002/cite.202300082</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Kosse, Pascal</span> ; <span
    style="font-variant:small-caps;">Hernández Rodriguez, Tanja</span> ; <span style="font-variant:small-caps;">Frahm,
    Björn</span> ; <span style="font-variant:small-caps;">Lübken, Manfred</span> ;
    <span style="font-variant:small-caps;">Wichern, Marc</span>: Validation and Uncertainty
    Assessment of a Software‐Integrated Workflow for pH Calculations. In: <i>Chemie
    Ingenieur Technik</i> Bd. 95. Weinheim, Wiley (2023), Nr. 12, S. 1960–1969'
  havard: P. Kosse, T. Hernández Rodriguez, B. Frahm, M. Lübken, M. Wichern, Validation
    and Uncertainty Assessment of a Software‐Integrated Workflow for pH Calculations,
    Chemie Ingenieur Technik. 95 (2023) 1960–1969.
  ieee: 'P. Kosse, T. Hernández Rodriguez, B. Frahm, M. Lübken, and M. Wichern, “Validation
    and Uncertainty Assessment of a Software‐Integrated Workflow for pH Calculations,”
    <i>Chemie Ingenieur Technik</i>, vol. 95, no. 12, pp. 1960–1969, 2023, doi: <a
    href="https://doi.org/10.1002/cite.202300082">10.1002/cite.202300082</a>.'
  mla: Kosse, Pascal, et al. “Validation and Uncertainty Assessment of a Software‐Integrated
    Workflow for PH Calculations.” <i>Chemie Ingenieur Technik</i>, vol. 95, no. 12,
    2023, pp. 1960–69, <a href="https://doi.org/10.1002/cite.202300082">https://doi.org/10.1002/cite.202300082</a>.
  short: P. Kosse, T. Hernández Rodriguez, B. Frahm, M. Lübken, M. Wichern, Chemie
    Ingenieur Technik 95 (2023) 1960–1969.
  ufg: '<b>Kosse, Pascal u. a.</b>: Validation and Uncertainty Assessment of a Software‐Integrated
    Workflow for pH Calculations, in: <i>Chemie Ingenieur Technik</i> 95 (2023), H.
    12,  S. 1960–1969.'
  van: Kosse P, Hernández Rodriguez T, Frahm B, Lübken M, Wichern M. Validation and
    Uncertainty Assessment of a Software‐Integrated Workflow for pH Calculations.
    Chemie Ingenieur Technik. 2023;95(12):1960–9.
date_created: 2024-04-26T06:52:48Z
date_updated: 2025-06-26T07:50:29Z
department:
- _id: DEP4000
doi: 10.1002/cite.202300082
external_id:
  isi:
  - '001073579300001'
intvolume: '        95'
isi: '1'
issue: '12'
keyword:
- Acid-base equilibria
- Carboxylic acids
- Dissociation constants
- pH calculation software
- Uncertainty assessment
language:
- iso: eng
page: 1960-1969
place: Weinheim
publication: Chemie Ingenieur Technik
publication_identifier:
  eissn:
  - 1522-2640
  issn:
  - 0009-286X
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: Validation and Uncertainty Assessment of a Software‐Integrated Workflow for
  pH Calculations
type: scientific_journal_article
user_id: '83781'
volume: 95
year: '2023'
...
---
_id: '2347'
author:
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
- first_name: Paul
  full_name: Lane, Paul
  last_name: Lane
- first_name: Axel
  full_name: Munack, Axel
  last_name: Munack
- first_name: Ralf
  full_name: Pörtner, Ralf
  last_name: Pörtner
citation:
  ama: Frahm B, Lane P, Munack A, Pörtner R. Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen
    mittels einer Kollokationsmethode. <i>Chemie Ingenieur Technik</i>. 2005;77(4):429-435.
    doi:<a href="https://doi.org/10.1002/cite.200407094">10.1002/cite.200407094</a>
  apa: Frahm, B., Lane, P., Munack, A., &#38; Pörtner, R. (2005). Optimierung und
    Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode.
    <i>Chemie Ingenieur Technik</i>, <i>77</i>(4), 429–435. <a href="https://doi.org/10.1002/cite.200407094">https://doi.org/10.1002/cite.200407094</a>
  bjps: <b>Frahm B <i>et al.</i></b> (2005) Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen
    mittels einer Kollokationsmethode. <i>Chemie Ingenieur Technik</i> <b>77</b>,
    429–435.
  chicago: 'Frahm, Björn, Paul Lane, Axel Munack, and Ralf Pörtner. “Optimierung und
    Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode.”
    <i>Chemie Ingenieur Technik</i> 77, no. 4 (2005): 429–35. <a href="https://doi.org/10.1002/cite.200407094">https://doi.org/10.1002/cite.200407094</a>.'
  chicago-de: 'Frahm, Björn, Paul Lane, Axel Munack und Ralf Pörtner. 2005. Optimierung
    und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode.
    <i>Chemie Ingenieur Technik</i> 77, Nr. 4: 429–435. doi:<a href="https://doi.org/10.1002/cite.200407094,">10.1002/cite.200407094,</a>
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Frahm, Björn</span> ; <span
    style="font-variant:small-caps;">Lane, Paul</span> ; <span style="font-variant:small-caps;">Munack,
    Axel</span> ; <span style="font-variant:small-caps;">Pörtner, Ralf</span>: Optimierung
    und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode.
    In: <i>Chemie Ingenieur Technik</i> Bd. 77, WILEY-VCH Verlag (2005), Nr. 4, S. 429–435'
  havard: B. Frahm, P. Lane, A. Munack, R. Pörtner, Optimierung und Steuerung von
    Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode, Chemie Ingenieur
    Technik. 77 (2005) 429–435.
  ieee: B. Frahm, P. Lane, A. Munack, and R. Pörtner, “Optimierung und Steuerung von
    Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode,” <i>Chemie Ingenieur
    Technik</i>, vol. 77, no. 4, pp. 429–435, 2005.
  mla: Frahm, Björn, et al. “Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen
    mittels einer Kollokationsmethode.” <i>Chemie Ingenieur Technik</i>, vol. 77,
    no. 4, WILEY-VCH Verlag, 2005, pp. 429–35, doi:<a href="https://doi.org/10.1002/cite.200407094">10.1002/cite.200407094</a>.
  short: B. Frahm, P. Lane, A. Munack, R. Pörtner, Chemie Ingenieur Technik 77 (2005)
    429–435.
  ufg: '<b>Frahm, Björn et. al. (2005)</b>: Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen
    mittels einer Kollokationsmethode, in: <i>Chemie Ingenieur Technik</i> <i>77</i>
    (<i>4</i>), S. 429–435.'
  van: Frahm B, Lane P, Munack A, Pörtner R. Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen
    mittels einer Kollokationsmethode. Chemie Ingenieur Technik. 2005;77(4):429–35.
date_created: 2020-05-15T10:06:16Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4021
doi: 10.1002/cite.200407094
extern: '1'
intvolume: '        77'
issue: '4'
language:
- iso: ger
main_file_link:
- open_access: '1'
  url: https://onlinelibrary.wiley.com/doi/10.1002/cite.200407094
oa: '1'
page: 429-435
publication: Chemie Ingenieur Technik
publication_identifier:
  eissn:
  - 1522-2640
  issn:
  - 0009-286X
publication_status: published
publisher: WILEY-VCH Verlag
status: public
title: Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer
  Kollokationsmethode
type: scientific_journal_article
user_id: '45666'
volume: 77
year: 2005
...
---
_id: '2343'
author:
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
- first_name: V. C.
  full_name: Hass, V. C.
  last_name: Hass
- first_name: P.
  full_name: Lane, P.
  last_name: Lane
- first_name: A.
  full_name: Munack, A.
  last_name: Munack
- first_name: H.
  full_name: Märkl, H.
  last_name: Märkl
- first_name: R.
  full_name: Pörtner, R.
  last_name: Pörtner
citation:
  ama: Frahm B, Hass VC, Lane P, Munack A, Märkl H, Pörtner R. Fed-Batch-Kultivierung
    tierischer Zellen - Eine Herausforderung zur adaptiven, modellbasierten Steuerung.
    <i>Chemie Ingenieur Technik</i>. 2003;75(4):457-460. doi:<a href="https://doi.org/10.1002/cite.200390093">10.1002/cite.200390093</a>
  apa: Frahm, B., Hass, V. C., Lane, P., Munack, A., Märkl, H., &#38; Pörtner, R.
    (2003). Fed-Batch-Kultivierung tierischer Zellen - Eine Herausforderung zur adaptiven,
    modellbasierten Steuerung. <i>Chemie Ingenieur Technik</i>, <i>75</i>(4), 457–460.
    <a href="https://doi.org/10.1002/cite.200390093">https://doi.org/10.1002/cite.200390093</a>
  bjps: <b>Frahm B <i>et al.</i></b> (2003) Fed-Batch-Kultivierung tierischer Zellen
    - Eine Herausforderung zur adaptiven, modellbasierten Steuerung. <i>Chemie Ingenieur
    Technik</i> <b>75</b>, 457–460.
  chicago: 'Frahm, Björn, V. C. Hass, P. Lane, A. Munack, H. Märkl, and R. Pörtner.
    “Fed-Batch-Kultivierung tierischer Zellen - Eine Herausforderung zur adaptiven,
    modellbasierten Steuerung.” <i>Chemie Ingenieur Technik</i> 75, no. 4 (2003):
    457–60. <a href="https://doi.org/10.1002/cite.200390093">https://doi.org/10.1002/cite.200390093</a>.'
  chicago-de: 'Frahm, Björn, V. C. Hass, P. Lane, A. Munack, H. Märkl und R. Pörtner.
    2003. Fed-Batch-Kultivierung tierischer Zellen - Eine Herausforderung zur adaptiven,
    modellbasierten Steuerung. <i>Chemie Ingenieur Technik</i> 75, Nr. 4: 457–460.
    doi:<a href="https://doi.org/10.1002/cite.200390093,">10.1002/cite.200390093,</a>
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Frahm, Björn</span> ; <span
    style="font-variant:small-caps;">Hass, V. C.</span> ; <span style="font-variant:small-caps;">Lane,
    P.</span> ; <span style="font-variant:small-caps;">Munack, A.</span> ; <span style="font-variant:small-caps;">Märkl,
    H.</span> ; <span style="font-variant:small-caps;">Pörtner, R.</span>: Fed-Batch-Kultivierung
    tierischer Zellen - Eine Herausforderung zur adaptiven, modellbasierten Steuerung.
    In: <i>Chemie Ingenieur Technik</i> Bd. 75, WILEY-VCH Verlag (2003), Nr. 4, S. 457–460'
  havard: B. Frahm, V.C. Hass, P. Lane, A. Munack, H. Märkl, R. Pörtner, Fed-Batch-Kultivierung
    tierischer Zellen - Eine Herausforderung zur adaptiven, modellbasierten Steuerung,
    Chemie Ingenieur Technik. 75 (2003) 457–460.
  ieee: B. Frahm, V. C. Hass, P. Lane, A. Munack, H. Märkl, and R. Pörtner, “Fed-Batch-Kultivierung
    tierischer Zellen - Eine Herausforderung zur adaptiven, modellbasierten Steuerung,”
    <i>Chemie Ingenieur Technik</i>, vol. 75, no. 4, pp. 457–460, 2003.
  mla: Frahm, Björn, et al. “Fed-Batch-Kultivierung tierischer Zellen - Eine Herausforderung
    zur adaptiven, modellbasierten Steuerung.” <i>Chemie Ingenieur Technik</i>, vol.
    75, no. 4, WILEY-VCH Verlag, 2003, pp. 457–60, doi:<a href="https://doi.org/10.1002/cite.200390093">10.1002/cite.200390093</a>.
  short: B. Frahm, V.C. Hass, P. Lane, A. Munack, H. Märkl, R. Pörtner, Chemie Ingenieur
    Technik 75 (2003) 457–460.
  ufg: '<b>Frahm, Björn et. al. (2003)</b>: Fed-Batch-Kultivierung tierischer Zellen
    - Eine Herausforderung zur adaptiven, modellbasierten Steuerung, in: <i>Chemie
    Ingenieur Technik</i> <i>75</i> (<i>4</i>), S. 457–460.'
  van: Frahm B, Hass VC, Lane P, Munack A, Märkl H, Pörtner R. Fed-Batch-Kultivierung
    tierischer Zellen - Eine Herausforderung zur adaptiven, modellbasierten Steuerung.
    Chemie Ingenieur Technik. 2003;75(4):457–60.
date_created: 2020-05-15T09:06:56Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4021
doi: 10.1002/cite.200390093
extern: '1'
intvolume: '        75'
issue: '4'
language:
- iso: ger
main_file_link:
- open_access: '1'
  url: https://www.onlinelibrary.wiley.com/doi/10.1002/cite.200390093
oa: '1'
page: 457-460
publication: Chemie Ingenieur Technik
publication_identifier:
  eissn:
  - 1522-2640
  issn:
  - 0009-286X
publication_status: published
publisher: WILEY-VCH Verlag
status: public
title: Fed-Batch-Kultivierung tierischer Zellen - Eine Herausforderung zur adaptiven,
  modellbasierten Steuerung
type: scientific_journal_article
user_id: '45666'
volume: 75
year: 2003
...