---
_id: '11218'
abstract:
- lang: eng
  text: An ultrafast microfluidic PCR module (30 PCR cycles in 6 min) based on the
    oscillating fluid plug concept was developed. A robust amplification of native
    genomic DNA from whole blood samples could be achieved at operational conditions
    established from systematic investigations of key parameters including heat transfer
    and in particular flow velocities. Experimental data were augmented with results
    from computational fluid dynamics simulations. The reproducibility of the current
    system was substantially improved compared to previous concepts by integration
    of a closed reservoir instead of utilizing a vented channel end at ambient pressure
    rendering the devised module suitable for integration into complex sample-to-answer
    analysis platforms such as point-of-care applications.
author:
- first_name: Sabine
  full_name: Brunklaus, Sabine
  last_name: Brunklaus
- first_name: Thomas
  full_name: Hansen-Hagge, Thomas
  last_name: Hansen-Hagge
- first_name: Julia
  full_name: Erwes, Julia
  last_name: Erwes
- first_name: Julian
  full_name: Höth, Julian
  last_name: Höth
- first_name: Matthieu
  full_name: Jung, Matthieu
  last_name: Jung
- first_name: Daniel
  full_name: Latta, Daniel
  last_name: Latta
- first_name: Xenia
  full_name: Strohbach, Xenia
  last_name: Strohbach
- first_name: Christian
  full_name: Winkler, Christian
  last_name: Winkler
- first_name: Marion
  full_name: Ritzi-Lehnert, Marion
  last_name: Ritzi-Lehnert
- first_name: Klaus S.
  full_name: Drese, Klaus S.
  last_name: Drese
citation:
  ama: 'Brunklaus S, Hansen-Hagge T, Erwes J, et al. Fast nucleic acid amplification
    for integration in point‐of‐care applications. <i>Electrophoresis : electrokinetics,
    fluidics, proteomics </i>. 2012;33(21):3222-3228. doi:<a href="https://doi.org/10.1002/elps.201200259">10.1002/elps.201200259</a>'
  apa: 'Brunklaus, S., Hansen-Hagge, T., Erwes, J., Höth, J., Jung, M., Latta, D.,
    Strohbach, X., Winkler, C., Ritzi-Lehnert, M., &#38; Drese, K. S. (2012). Fast
    nucleic acid amplification for integration in point‐of‐care applications. <i>Electrophoresis :
    Electrokinetics, Fluidics, Proteomics </i>, <i>33</i>(21), 3222–3228. <a href="https://doi.org/10.1002/elps.201200259">https://doi.org/10.1002/elps.201200259</a>'
  bjps: '<b>Brunklaus S <i>et al.</i></b> (2012) Fast Nucleic Acid Amplification for
    Integration in Point‐of‐care Applications. <i>Electrophoresis : electrokinetics,
    fluidics, proteomics </i> <b>33</b>, 3222–3228.'
  chicago: 'Brunklaus, Sabine, Thomas Hansen-Hagge, Julia Erwes, Julian Höth, Matthieu
    Jung, Daniel Latta, Xenia Strohbach, Christian Winkler, Marion Ritzi-Lehnert,
    and Klaus S. Drese. “Fast Nucleic Acid Amplification for Integration in Point‐of‐care
    Applications.” <i>Electrophoresis : Electrokinetics, Fluidics, Proteomics </i>
    33, no. 21 (2012): 3222–28. <a href="https://doi.org/10.1002/elps.201200259">https://doi.org/10.1002/elps.201200259</a>.'
  chicago-de: 'Brunklaus, Sabine, Thomas Hansen-Hagge, Julia Erwes, Julian Höth, Matthieu
    Jung, Daniel Latta, Xenia Strohbach, Christian Winkler, Marion Ritzi-Lehnert und
    Klaus S. Drese. 2012. Fast nucleic acid amplification for integration in point‐of‐care
    applications. <i>Electrophoresis : electrokinetics, fluidics, proteomics </i>
    33, Nr. 21: 3222–3228. doi:<a href="https://doi.org/10.1002/elps.201200259">10.1002/elps.201200259</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Brunklaus,
    Sabine</span> ; <span style="font-variant:small-caps;">Hansen-Hagge, Thomas</span>
    ; <span style="font-variant:small-caps;">Erwes, Julia</span> ; <span style="font-variant:small-caps;">Höth,
    Julian</span> ; <span style="font-variant:small-caps;">Jung, Matthieu</span> ;
    <span style="font-variant:small-caps;">Latta, Daniel</span> ; <span style="font-variant:small-caps;">Strohbach,
    Xenia</span> ; <span style="font-variant:small-caps;">Winkler, Christian</span>
    ; u. a.</span>: Fast nucleic acid amplification for integration in point‐of‐care
    applications. In: <i>Electrophoresis : electrokinetics, fluidics, proteomics </i>
    Bd. 33. Weinheim, Wiley-VCH (2012), Nr. 21, S. 3222–3228'
  havard: 'S. Brunklaus, T. Hansen-Hagge, J. Erwes, J. Höth, M. Jung, D. Latta, X.
    Strohbach, C. Winkler, M. Ritzi-Lehnert, K.S. Drese, Fast nucleic acid amplification
    for integration in point‐of‐care applications, Electrophoresis : Electrokinetics,
    Fluidics, Proteomics . 33 (2012) 3222–3228.'
  ieee: 'S. Brunklaus <i>et al.</i>, “Fast nucleic acid amplification for integration
    in point‐of‐care applications,” <i>Electrophoresis : electrokinetics, fluidics,
    proteomics </i>, vol. 33, no. 21, pp. 3222–3228, 2012, doi: <a href="https://doi.org/10.1002/elps.201200259">10.1002/elps.201200259</a>.'
  mla: 'Brunklaus, Sabine, et al. “Fast Nucleic Acid Amplification for Integration
    in Point‐of‐care Applications.” <i>Electrophoresis : Electrokinetics, Fluidics,
    Proteomics </i>, vol. 33, no. 21, 2012, pp. 3222–28, <a href="https://doi.org/10.1002/elps.201200259">https://doi.org/10.1002/elps.201200259</a>.'
  short: 'S. Brunklaus, T. Hansen-Hagge, J. Erwes, J. Höth, M. Jung, D. Latta, X.
    Strohbach, C. Winkler, M. Ritzi-Lehnert, K.S. Drese, Electrophoresis : Electrokinetics,
    Fluidics, Proteomics  33 (2012) 3222–3228.'
  ufg: '<b>Brunklaus, Sabine u. a.</b>: Fast nucleic acid amplification for integration
    in point‐of‐care applications, in: <i>Electrophoresis : electrokinetics, fluidics,
    proteomics </i> 33 (2012), H. 21,  S. 3222–3228.'
  van: 'Brunklaus S, Hansen-Hagge T, Erwes J, Höth J, Jung M, Latta D, et al. Fast
    nucleic acid amplification for integration in point‐of‐care applications. Electrophoresis :
    electrokinetics, fluidics, proteomics . 2012;33(21):3222–8.'
date_created: 2024-03-16T19:00:18Z
date_updated: 2024-06-26T13:52:14Z
doi: 10.1002/elps.201200259
intvolume: '        33'
issue: '21'
language:
- iso: eng
page: 3222-3228
place: Weinheim
publication: 'Electrophoresis : electrokinetics, fluidics, proteomics '
publication_identifier:
  eissn:
  - 1522-2683
  issn:
  - 0173-0835
publication_status: published
publisher: Wiley-VCH
status: public
title: Fast nucleic acid amplification for integration in point‐of‐care applications
type: scientific_journal_article
user_id: '65129'
volume: 33
year: '2012'
...