--- _id: '14057' abstract: - lang: eng text: Bioactive peptides from plant proteins offer significant benefits. In this study, the structural and bioactive properties of sunflower protein isolate and its enzymatic hydrolysates were systematically investigated. The hydrolysates were fractionated using low-pressure preparative gradient chromatography (LP-PGC), and their antioxidant capacity, along with angiotensin-converting enzyme and dipeptidyl peptidase-IV inhibitory activities relevant to metabolic health, were assessed. Structural analyses via sodium dodecyl sulfate polyacrylamide gel electrophoresis and scanning electron microscopy revealed significant alterations in molecular weight distribution and surface morphology after hydrolysis and fractionation, confirming the generation of low-molecular-weight peptides linked to improved bioactivity. The results demonstrated a clear fraction-dependent bioactivity profile. MTT-based cell viability analysis of HepG2 and Caco-2 cells indicates that the non-toxicity of the hydrolysates depends on both their concentration and the cell type. Molecular docking studies were performed with the three highest-scoring peptides from each hydrolysate according to PeptideRanker. LP-PGC effectively reveals in vitro/in silico bioactivity of sunflower protein hydrolysates for multifunctional food use. article_number: '150976' author: - first_name: Zeynep Saliha full_name: Güneş, Zeynep Saliha last_name: Güneş - first_name: İbrahim full_name: Gülseren, İbrahim last_name: Gülseren - first_name: Carsten full_name: Funke, Carsten id: '74167' last_name: Funke - first_name: Hans-Jürgen full_name: Danneel, Hans-Jürgen id: '12231' last_name: Danneel - first_name: Tuba full_name: Esatbeyoglu, Tuba last_name: Esatbeyoglu citation: ama: 'Güneş ZS, Gülseren İ, Funke C, Danneel HJ, Esatbeyoglu T. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. Food Chemistry. 2026;528. doi:10.1016/j.foodchem.2026.150976' apa: 'Güneş, Z. S., Gülseren, İ., Funke, C., Danneel, H.-J., & Esatbeyoglu, T. (2026). Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. Food Chemistry, 528, Article 150976. https://doi.org/10.1016/j.foodchem.2026.150976' bjps: 'Güneş ZS et al. (2026) Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities. Food Chemistry 528.' chicago: 'Güneş, Zeynep Saliha, İbrahim Gülseren, Carsten Funke, Hans-Jürgen Danneel, and Tuba Esatbeyoglu. “Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities.” Food Chemistry 528 (2026). https://doi.org/10.1016/j.foodchem.2026.150976.' chicago-de: 'Güneş, Zeynep Saliha, İbrahim Gülseren, Carsten Funke, Hans-Jürgen Danneel und Tuba Esatbeyoglu. 2026. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. Food Chemistry 528. doi:10.1016/j.foodchem.2026.150976, .' din1505-2-1: 'Güneş, Zeynep Saliha ; Gülseren, İbrahim ; Funke, Carsten ; Danneel, Hans-Jürgen ; Esatbeyoglu, Tuba: Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. In: Food Chemistry Bd. 528. New York, NY [u.a.], Elsevier BV (2026)' havard: 'Z.S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, T. Esatbeyoglu, Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities, Food Chemistry. 528 (2026).' ieee: 'Z. S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, and T. Esatbeyoglu, “Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities,” Food Chemistry, vol. 528, Art. no. 150976, 2026, doi: 10.1016/j.foodchem.2026.150976.' mla: 'Güneş, Zeynep Saliha, et al. “Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities.” Food Chemistry, vol. 528, 150976, 2026, https://doi.org/10.1016/j.foodchem.2026.150976.' short: Z.S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, T. Esatbeyoglu, Food Chemistry 528 (2026). ufg: 'Güneş, Zeynep Saliha u. a.: Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities, in: Food Chemistry 528 (2026).' van: 'Güneş ZS, Gülseren İ, Funke C, Danneel HJ, Esatbeyoglu T. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. Food Chemistry. 2026;528.' date_created: 2026-09-24T07:39:35Z date_updated: 2026-09-25T09:08:20Z department: - _id: DEP4018 doi: 10.1016/j.foodchem.2026.150976 intvolume: ' 528' keyword: - Sunflower protein hydrolysates - Fractionation - Bioactive peptides - ACE inhibition - DPP-IV inhibition - Mass spectrometry - In silico analysis language: - iso: eng place: New York, NY [u.a.] publication: Food Chemistry publication_identifier: eissn: - 1873-7072 issn: - 0308-8146 publication_status: published publisher: Elsevier BV quality_controlled: '1' status: public title: 'Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities' type: scientific_journal_article user_id: '83781' volume: 528 year: '2026' ...