[{"user_id":"83781","article_number":"1984","date_updated":"2025-06-16T09:16:39Z","year":"2023","language":[{"iso":"eng"}],"date_created":"2025-06-15T09:35:35Z","status":"public","author":[{"first_name":"Heidrun","full_name":"Gehlen, Heidrun","last_name":"Gehlen"},{"first_name":"Katharina","last_name":"Inerle","full_name":"Inerle, Katharina"},{"first_name":"Alexander","last_name":"Bartel","full_name":"Bartel, Alexander"},{"first_name":"Sabita Diana","last_name":"Stöckle","full_name":"Stöckle, Sabita Diana"},{"orcid":"0000-0002-4511-9537","first_name":"Sebastian","last_name":"Ulrich","full_name":"Ulrich, Sebastian","id":"85847"},{"full_name":"Briese, Beatrice","last_name":"Briese","first_name":"Beatrice"},{"full_name":"Straubinger, Reinhard K.","last_name":"Straubinger","first_name":"Reinhard K."}],"extern":"1","place":"Basel","abstract":[{"lang":"eng","text":"There are limited data on Lyme borreliosis (LB), a tick-borne disease caused by the Borrelia burgdorferi sensu lato complex, in horses. Seropositivity is not necessarily associated with clinical disease. Data on seropositivity against Borrelia burgdorferi and Anaplasma phagocytophilum in German horses are sparse. Therefore, serum samples from horses (n = 123) suspected of having Lyme borreliosis and clinically healthy horses (n = 113) from the same stables were tested for specific antibodies against Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum. The samples were screened for antibodies against Borrelia burgdorferi (ELISA and an IgG line immunoblot assay). Furthermore, the samples were examined for antibodies against B. burgdorferi and Anaplasma phagocytophilum with a validated rapid in-house test (SNAP® 4Dx Plus® ELISA). The clinical signs of suspect horses included lameness (n = 36), poor performance (n = 19), and apathy (n = 12). Twenty-three percent (n = 26) of suspect horses and 17% (n = 18) of clinically healthy horses were seropositive for having a Borrelia burgdorferi sensu lato infection (p = 0.371), showing that the detection of specific antibodies against B. burgdorferi alone is not sufficient for a diagnosis of equine LB. Anaplasma phagocytophilum seropositivity and seropositivity against both pathogens was 20%/6% in suspect horses and 16%/2% in the clinically healthy population, showing only minor differences (p = 0.108). Unspecific testing for antibodies against B. burgdorferi without clinical suspicion of Lyme borreliosis is not recommended since the clinical relevance of seropositivity against Borrelia burgdorferi sensu lato remains to be elucidated."}],"volume":13,"_id":"12940","type":"scientific_journal_article","keyword":["equine Lyme borreliosis","equine granulocytic anaplasmosis","seroprevalence","co-infection"],"department":[{"_id":"DEP4010"}],"title":"Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses","publication_status":"published","publication_identifier":{"eissn":["2076-2615"]},"issue":"12","doi":"10.3390/ani13121984","publication":"Animals","intvolume":"        13","citation":{"bjps":"<b>Gehlen H <i>et al.</i></b> (2023) Seroprevalence of Borrelia Burgdorferi Sensu Lato and Anaplasma Phagocytophilum Infections in German Horses. <i>Animals</i> <b>13</b>.","chicago-de":"Gehlen, Heidrun, Katharina Inerle, Alexander Bartel, Sabita Diana Stöckle, Sebastian Ulrich, Beatrice Briese und Reinhard K. Straubinger. 2023. Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses. <i>Animals</i> 13, Nr. 12. doi:<a href=\"https://doi.org/10.3390/ani13121984\">10.3390/ani13121984</a>, .","din1505-2-1":"<span style=\"font-variant:small-caps;\">Gehlen, Heidrun</span> ; <span style=\"font-variant:small-caps;\">Inerle, Katharina</span> ; <span style=\"font-variant:small-caps;\">Bartel, Alexander</span> ; <span style=\"font-variant:small-caps;\">Stöckle, Sabita Diana</span> ; <span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Briese, Beatrice</span> ; <span style=\"font-variant:small-caps;\">Straubinger, Reinhard K.</span>: Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses. In: <i>Animals</i> Bd. 13. Basel, MDPI AG (2023), Nr. 12","chicago":"Gehlen, Heidrun, Katharina Inerle, Alexander Bartel, Sabita Diana Stöckle, Sebastian Ulrich, Beatrice Briese, and Reinhard K. Straubinger. “Seroprevalence of Borrelia Burgdorferi Sensu Lato and Anaplasma Phagocytophilum Infections in German Horses.” <i>Animals</i> 13, no. 12 (2023). <a href=\"https://doi.org/10.3390/ani13121984\">https://doi.org/10.3390/ani13121984</a>.","short":"H. Gehlen, K. Inerle, A. Bartel, S.D. Stöckle, S. Ulrich, B. Briese, R.K. Straubinger, Animals 13 (2023).","mla":"Gehlen, Heidrun, et al. “Seroprevalence of Borrelia Burgdorferi Sensu Lato and Anaplasma Phagocytophilum Infections in German Horses.” <i>Animals</i>, vol. 13, no. 12, 1984, 2023, <a href=\"https://doi.org/10.3390/ani13121984\">https://doi.org/10.3390/ani13121984</a>.","van":"Gehlen H, Inerle K, Bartel A, Stöckle SD, Ulrich S, Briese B, et al. Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses. Animals. 2023;13(12).","ieee":"H. Gehlen <i>et al.</i>, “Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses,” <i>Animals</i>, vol. 13, no. 12, Art. no. 1984, 2023, doi: <a href=\"https://doi.org/10.3390/ani13121984\">10.3390/ani13121984</a>.","apa":"Gehlen, H., Inerle, K., Bartel, A., Stöckle, S. D., Ulrich, S., Briese, B., &#38; Straubinger, R. K. (2023). Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses. <i>Animals</i>, <i>13</i>(12), Article 1984. <a href=\"https://doi.org/10.3390/ani13121984\">https://doi.org/10.3390/ani13121984</a>","ama":"Gehlen H, Inerle K, Bartel A, et al. Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses. <i>Animals</i>. 2023;13(12). doi:<a href=\"https://doi.org/10.3390/ani13121984\">10.3390/ani13121984</a>","ufg":"<b>Gehlen, Heidrun u. a.</b>: Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses, in: <i>Animals</i> 13 (2023), H. 12.","havard":"H. Gehlen, K. Inerle, A. Bartel, S.D. Stöckle, S. Ulrich, B. Briese, R.K. Straubinger, Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses, Animals. 13 (2023)."},"publisher":"MDPI AG","quality_controlled":"1"},{"abstract":[{"lang":"eng","text":"Diet processing impacts on starch properties, such as the degree of starch gelatinization. This affects digestibility, as shown in laboratory mice fed either a pelleted or an extruded diet. In the present study, the morphology of starch particles throughout the digestive tract of mice was visualized. Thirty-two female C57BL/6J mice were used for a feeding trial. They were fed a commercial maintenance diet for laboratory mice, which was available in pelleted and extruded form, for seven weeks. The mice were sacrificed after the feeding period, and chyme samples were collected from five sites (stomach, anterior and posterior small intestine, caecum, colon). Samples of diets, chyme and faeces were analyzed via stereomicroscopy (stained with Lugol’s iodine) and scanning electron microscopy (SEM). The starch granules appeared more compact in the pelleted diet, showing first signs of degradation only in the small intestine. The caecum content of both diets group was intensively stained, particles as well as fluid phase, indicating that it contained mainly starch. The SEM pictures of caecum content showed abundant bacteria near starch particles. This suggests selective retention of prae-caecally undigested starch in the murine caecum, likely the site of microbial fermentation."}],"volume":12,"_id":"12948","type":"scientific_journal_article","status":"public","author":[{"full_name":"Wenderlein, Jasmin","last_name":"Wenderlein","first_name":"Jasmin"},{"last_name":"Kienzle","full_name":"Kienzle, Ellen","first_name":"Ellen"},{"first_name":"Reinhard K.","full_name":"Straubinger, Reinhard K.","last_name":"Straubinger"},{"first_name":"Heidrun","last_name":"Schöl","full_name":"Schöl, Heidrun"},{"last_name":"Ulrich","full_name":"Ulrich, Sebastian","id":"85847","orcid":"0000-0002-4511-9537","first_name":"Sebastian"},{"full_name":"Böswald, Linda Franziska","last_name":"Böswald","first_name":"Linda Franziska"}],"extern":"1","place":"Basel","language":[{"iso":"eng"}],"date_created":"2025-06-15T09:57:56Z","article_number":"952","user_id":"83781","year":"2022","date_updated":"2025-06-16T12:01:46Z","publisher":"MDPI AG","quality_controlled":"1","citation":{"chicago-de":"Wenderlein, Jasmin, Ellen Kienzle, Reinhard K. Straubinger, Heidrun Schöl, Sebastian Ulrich und Linda Franziska Böswald. 2022. Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets. <i>Animals</i> 12, Nr. 8. doi:<a href=\"https://doi.org/10.3390/ani12080952\">10.3390/ani12080952</a>, .","bjps":"<b>Wenderlein J <i>et al.</i></b> (2022) Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets. <i>Animals</i> <b>12</b>.","van":"Wenderlein J, Kienzle E, Straubinger RK, Schöl H, Ulrich S, Böswald LF. Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets. Animals. 2022;12(8).","ieee":"J. Wenderlein, E. Kienzle, R. K. Straubinger, H. Schöl, S. Ulrich, and L. F. Böswald, “Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets,” <i>Animals</i>, vol. 12, no. 8, Art. no. 952, 2022, doi: <a href=\"https://doi.org/10.3390/ani12080952\">10.3390/ani12080952</a>.","apa":"Wenderlein, J., Kienzle, E., Straubinger, R. K., Schöl, H., Ulrich, S., &#38; Böswald, L. F. (2022). Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets. <i>Animals</i>, <i>12</i>(8), Article 952. <a href=\"https://doi.org/10.3390/ani12080952\">https://doi.org/10.3390/ani12080952</a>","din1505-2-1":"<span style=\"font-variant:small-caps;\">Wenderlein, Jasmin</span> ; <span style=\"font-variant:small-caps;\">Kienzle, Ellen</span> ; <span style=\"font-variant:small-caps;\">Straubinger, Reinhard K.</span> ; <span style=\"font-variant:small-caps;\">Schöl, Heidrun</span> ; <span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Böswald, Linda Franziska</span>: Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets. In: <i>Animals</i> Bd. 12. Basel, MDPI AG (2022), Nr. 8","short":"J. Wenderlein, E. Kienzle, R.K. Straubinger, H. Schöl, S. Ulrich, L.F. Böswald, Animals 12 (2022).","chicago":"Wenderlein, Jasmin, Ellen Kienzle, Reinhard K. Straubinger, Heidrun Schöl, Sebastian Ulrich, and Linda Franziska Böswald. “Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets.” <i>Animals</i> 12, no. 8 (2022). <a href=\"https://doi.org/10.3390/ani12080952\">https://doi.org/10.3390/ani12080952</a>.","ama":"Wenderlein J, Kienzle E, Straubinger RK, Schöl H, Ulrich S, Böswald LF. Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets. <i>Animals</i>. 2022;12(8). doi:<a href=\"https://doi.org/10.3390/ani12080952\">10.3390/ani12080952</a>","mla":"Wenderlein, Jasmin, et al. “Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets.” <i>Animals</i>, vol. 12, no. 8, 952, 2022, <a href=\"https://doi.org/10.3390/ani12080952\">https://doi.org/10.3390/ani12080952</a>.","ufg":"<b>Wenderlein, Jasmin u. a.</b>: Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets, in: <i>Animals</i> 12 (2022), H. 8.","havard":"J. Wenderlein, E. Kienzle, R.K. Straubinger, H. Schöl, S. Ulrich, L.F. Böswald, Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets, Animals. 12 (2022)."},"intvolume":"        12","issue":"8","doi":"10.3390/ani12080952","title":"Morphology of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets","department":[{"_id":"DEP4010"}],"publication_identifier":{"eissn":["2076-2615"]},"publication_status":"published","publication":"Animals","keyword":["amylase","carbohydrate metabolism","processing","laboratory animal diets","caecum fermentation"]},{"issue":"2","doi":"10.3390/ani11020523","title":"Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility","department":[{"_id":"DEP4010"}],"publication_status":"published","publication_identifier":{"eissn":["2076-2615"]},"publication":"Animals","keyword":["standardization","carbohydrate digestibility","feed processing","starch gelatinization","gut"],"publisher":"MDPI","quality_controlled":"1","citation":{"apa":"Böswald, L. F., Wenderlein, J., Straubinger, R. K., Ulrich, S., &#38; Kienzle, E. (2021). Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility. <i>Animals</i>, <i>11</i>(2), Article 523. <a href=\"https://doi.org/10.3390/ani11020523\">https://doi.org/10.3390/ani11020523</a>","ama":"Böswald LF, Wenderlein J, Straubinger RK, Ulrich S, Kienzle E. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility. <i>Animals</i>. 2021;11(2). doi:<a href=\"https://doi.org/10.3390/ani11020523\">10.3390/ani11020523</a>","ufg":"<b>Böswald, Linda F. u. a.</b>: Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility, in: <i>Animals</i> 11 (2021), H. 2.","havard":"L.F. Böswald, J. Wenderlein, R.K. Straubinger, S. Ulrich, E. Kienzle, Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility, Animals. 11 (2021).","ieee":"L. F. Böswald, J. Wenderlein, R. K. Straubinger, S. Ulrich, and E. Kienzle, “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility,” <i>Animals</i>, vol. 11, no. 2, Art. no. 523, 2021, doi: <a href=\"https://doi.org/10.3390/ani11020523\">10.3390/ani11020523</a>.","van":"Böswald LF, Wenderlein J, Straubinger RK, Ulrich S, Kienzle E. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility. Animals. 2021;11(2).","din1505-2-1":"<span style=\"font-variant:small-caps;\">Böswald, Linda F.</span> ; <span style=\"font-variant:small-caps;\">Wenderlein, Jasmin</span> ; <span style=\"font-variant:small-caps;\">Straubinger, Reinhard K.</span> ; <span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Kienzle, Ellen</span>: Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility. In: <i>Animals</i> Bd. 11. Basel, MDPI (2021), Nr. 2","mla":"Böswald, Linda F., et al. “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility.” <i>Animals</i>, vol. 11, no. 2, 523, 2021, <a href=\"https://doi.org/10.3390/ani11020523\">https://doi.org/10.3390/ani11020523</a>.","chicago":"Böswald, Linda F., Jasmin Wenderlein, Reinhard K. Straubinger, Sebastian Ulrich, and Ellen Kienzle. “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility.” <i>Animals</i> 11, no. 2 (2021). <a href=\"https://doi.org/10.3390/ani11020523\">https://doi.org/10.3390/ani11020523</a>.","short":"L.F. Böswald, J. Wenderlein, R.K. Straubinger, S. Ulrich, E. Kienzle, Animals 11 (2021).","bjps":"<b>Böswald LF <i>et al.</i></b> (2021) Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility. <i>Animals</i> <b>11</b>.","chicago-de":"Böswald, Linda F., Jasmin Wenderlein, Reinhard K. Straubinger, Sebastian Ulrich und Ellen Kienzle. 2021. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility. <i>Animals</i> 11, Nr. 2. doi:<a href=\"https://doi.org/10.3390/ani11020523\">10.3390/ani11020523</a>, ."},"intvolume":"        11","language":[{"iso":"eng"}],"date_created":"2025-06-15T10:00:16Z","article_number":"523","user_id":"83781","year":"2021","date_updated":"2025-06-16T12:27:09Z","abstract":[{"lang":"eng","text":"Starch gelatinization is a major determinant of carbohydrate digestibility and varies with diet processing. Laboratory rodent diets are often marketed as identical, but are sold in different forms, regardless of the markedly higher starch gelatinization in extruded than in pelleted diets. Our hypothesis was that this would impact energy and nutrient digestibility in mice fed pellets or extrudate, respectively. Trial 1 showed that feeding C57BL/6 mice a standard maintenance diet in extruded form results in a significantly higher digestibility of organic matter, energy, and carbohydrates than the identical diet in pelleted form. The replication of the experiment, however, revealed a variation between batches of the same pelleted diet regarding starch and total dietary fiber contents. Given the significant differences in diet digestibility and the potential impacts of digestibility on nutrient utilization, the intestinal microbiome, and intermediary metabolism, trials performed with differently processed diets are not comparable. This might partly explain failures to reproduce results, especially in gastrointestinal or microbiome research. Considering this impact on experimental animals, the degree of starch gelatinization should be declared in the diet information for laboratory animal diets. The differences between batches of laboratory animal diets as observed in the pellets are not acceptable."}],"volume":11,"_id":"12953","type":"scientific_journal_article","status":"public","author":[{"full_name":"Böswald, Linda F.","last_name":"Böswald","first_name":"Linda F."},{"first_name":"Jasmin","last_name":"Wenderlein","full_name":"Wenderlein, Jasmin"},{"first_name":"Reinhard K.","last_name":"Straubinger","full_name":"Straubinger, Reinhard K."},{"orcid":"0000-0002-4511-9537","first_name":"Sebastian","last_name":"Ulrich","full_name":"Ulrich, Sebastian","id":"85847"},{"full_name":"Kienzle, Ellen","last_name":"Kienzle","first_name":"Ellen"}],"extern":"1","place":"Basel"}]