---
_id: '12944'
abstract:
- lang: eng
  text: Lyme borreliosis is a vector-borne disease in humans and animals caused by
    bacteria from the Borrelia burgdorferi sensu lato complex (Bbsl). The possible
    transmission of Bbsl from companion animals to humans via ticks makes this disease
    important in terms of One Health approaches. Thus, early and accurate diagnosis
    and treatment are of utmost importance. Today’s standard for the detection of
    specific antibodies against Bbsl is a two-tiered test system based on an ELISA
    for screening combined with a line immunoassay (LIA) for confirmation. In this
    study, 200 canine and 200 equine serum samples with known antibody status were
    tested with two different LIAs (A and B). Results were compared regarding sensitivity,
    specificity, the diagnostic outcome for dogs and horses, as well as operability
    of the test. The results for canine serum samples corresponded to 94.0%, making
    both LIAs a good choice for LB diagnostic in dogs. For equine serum samples, the
    agreement of both tests was 65.5%, displaying the challenge equine samples still
    provide in LB diagnostic. Major concerns were the interpretation of the OspA antigen
    (AG) signal and the use of unspecific (i.e., p100/p83) or too sensitive signals
    on the LIA. The operability of both LIAs was equally user-friendly. Regarding
    the tests’ evaluation, the scanning process provided by LIA A was a major advantage
    considering the comparability of the tests.
article_number: '633'
author:
- first_name: Sophie
  full_name: Doff, Sophie
  last_name: Doff
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Anna
  full_name: Wiesinger, Anna
  last_name: Wiesinger
- first_name: Stephanie
  full_name: Hiereth, Stephanie
  last_name: Hiereth
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Reinhard
  full_name: Straubinger, Reinhard
  last_name: Straubinger
citation:
  ama: 'Doff S, Wenderlein J, Wiesinger A, Hiereth S, Ulrich S, Straubinger R. Detection
    of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of Canine and Equine
    Origin—A Comparative Study with Two Line Immunoassays. <i>Veterinary Sciences :
    open access journal</i>. 2022;9(11). doi:<a href="https://doi.org/10.3390/vetsci9110633">10.3390/vetsci9110633</a>'
  apa: 'Doff, S., Wenderlein, J., Wiesinger, A., Hiereth, S., Ulrich, S., &#38; Straubinger,
    R. (2022). Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies in
    Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays.
    <i>Veterinary Sciences : Open Access Journal</i>, <i>9</i>(11), Article 633. <a
    href="https://doi.org/10.3390/vetsci9110633">https://doi.org/10.3390/vetsci9110633</a>'
  bjps: '<b>Doff S <i>et al.</i></b> (2022) Detection of Borrelia Burgdorferi Sensu-Lato-Specific
    Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line
    Immunoassays. <i>Veterinary Sciences : open access journal</i> <b>9</b>.'
  chicago: 'Doff, Sophie, Jasmin Wenderlein, Anna Wiesinger, Stephanie Hiereth, Sebastian
    Ulrich, and Reinhard Straubinger. “Detection of Borrelia Burgdorferi Sensu-Lato-Specific
    Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line
    Immunoassays.” <i>Veterinary Sciences : Open Access Journal</i> 9, no. 11 (2022).
    <a href="https://doi.org/10.3390/vetsci9110633">https://doi.org/10.3390/vetsci9110633</a>.'
  chicago-de: 'Doff, Sophie, Jasmin Wenderlein, Anna Wiesinger, Stephanie Hiereth,
    Sebastian Ulrich und Reinhard Straubinger. 2022. Detection of Borrelia burgdorferi
    sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative
    Study with Two Line Immunoassays. <i>Veterinary Sciences : open access journal</i>
    9, Nr. 11. doi:<a href="https://doi.org/10.3390/vetsci9110633">10.3390/vetsci9110633</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Doff, Sophie</span> ; <span
    style="font-variant:small-caps;">Wenderlein, Jasmin</span> ; <span style="font-variant:small-caps;">Wiesinger,
    Anna</span> ; <span style="font-variant:small-caps;">Hiereth, Stephanie</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Straubinger,
    Reinhard</span>: Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies
    in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays.
    In: <i>Veterinary Sciences : open access journal</i> Bd. 9. Basel, MDPI (2022),
    Nr. 11'
  havard: 'S. Doff, J. Wenderlein, A. Wiesinger, S. Hiereth, S. Ulrich, R. Straubinger,
    Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of Canine
    and Equine Origin—A Comparative Study with Two Line Immunoassays, Veterinary Sciences :
    Open Access Journal. 9 (2022).'
  ieee: 'S. Doff, J. Wenderlein, A. Wiesinger, S. Hiereth, S. Ulrich, and R. Straubinger,
    “Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of Canine
    and Equine Origin—A Comparative Study with Two Line Immunoassays,” <i>Veterinary
    Sciences : open access journal</i>, vol. 9, no. 11, Art. no. 633, 2022, doi: <a
    href="https://doi.org/10.3390/vetsci9110633">10.3390/vetsci9110633</a>.'
  mla: 'Doff, Sophie, et al. “Detection of Borrelia Burgdorferi Sensu-Lato-Specific
    Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line
    Immunoassays.” <i>Veterinary Sciences : Open Access Journal</i>, vol. 9, no. 11,
    633, 2022, <a href="https://doi.org/10.3390/vetsci9110633">https://doi.org/10.3390/vetsci9110633</a>.'
  short: 'S. Doff, J. Wenderlein, A. Wiesinger, S. Hiereth, S. Ulrich, R. Straubinger,
    Veterinary Sciences : Open Access Journal 9 (2022).'
  ufg: '<b>Doff, Sophie u. a.</b>: Detection of Borrelia burgdorferi sensu-lato-Specific
    Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line
    Immunoassays, in: <i>Veterinary Sciences : open access journal</i> 9 (2022), H.
    11.'
  van: 'Doff S, Wenderlein J, Wiesinger A, Hiereth S, Ulrich S, Straubinger R. Detection
    of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of Canine and Equine
    Origin—A Comparative Study with Two Line Immunoassays. Veterinary Sciences : open
    access journal. 2022;9(11).'
date_created: 2025-06-15T09:54:05Z
date_updated: 2025-06-16T11:49:06Z
department:
- _id: DEP4010
doi: 10.3390/vetsci9110633
extern: '1'
intvolume: '         9'
issue: '11'
keyword:
- antibody
- Borrelia burgdorferi sensu lato
- canine
- equine
- serum diagnosis
- line immunoassay
language:
- iso: eng
place: Basel
publication: 'Veterinary Sciences : open access journal'
publication_identifier:
  eissn:
  - 2306-7381
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of
  Canine and Equine Origin—A Comparative Study with Two Line Immunoassays
type: scientific_journal_article
user_id: '83781'
volume: 9
year: '2022'
...