---
_id: '12896'
abstract:
- lang: eng
text: 'Poly-3-hydroxybutyrate (P3HB) is a promising alternative to persistent conventional
plastics, capable of biodegrading within months. However, its microbial-driven
degradation raises concerns about nutrient immobilization and impacts on plant
growth. The biodegradation process occurs in multiple stages, during which shifts
in the microbial community can alter soil properties and influence utilization
of both intrinsic and polymer-derived organic matter. This study employs a novel
approach to investigate how nutrient dynamics during the late stage of P3HB biodegradation
affect lettuce (Lactuca sativa var. capitata cv. Brilliant) growth. Soil-to-sand
mixtures (100_0, 80_20, 60_40, 40_60, 20_80, and 0_100 ratios) were spiked with
P3HB, allowed to biodegrade for eight weeks, and then planted with sprouted lettuce
seeds, which were cultivated for another eight weeks. P3HB addition inhibited
plant growth and root development in all soil-sand mixtures. However, increasing
the sand proportion enhanced plants'' nitrogen content by 13-45 % compared to
100 % soil + P3HB. Depending on the sand-to-soil ratio, P3HB stimulated most enzymes
involved in carbon, nitrogen and phosphorus acquisition. Basal and substrate-induced
respirations were 9-209 % higher under P3HB addition compared to P3HB-free soil,
likely due to an increase in the stabilized soil organic matter fraction. Residual
P3HB analysis revealed that diluting soil with 20 % sand accelerated biodegradation,
despite a decrease in bacterial abundance. In the 80_20 variant, the microbial
community shifted toward higher fungal abundance, 19 % more than in 100_0 soil.
While microbial proliferation was observed, it effect was outweighed by negative
impacts on dry aboveground and root biomass. The highest P3HB biodegradation rate
occurred in the 80_20 variant, underscoring soil texture as a critical factor
in P3HB biodegradation. While microbial communities can degrade bioplastics, this
process may compromise plant nutrient availability and hinder plant growth. '
article_number: '121618'
author:
- first_name: Martin
full_name: Brtnicky, Martin
last_name: Brtnicky
- first_name: Adnan
full_name: Mustafa, Adnan
last_name: Mustafa
- first_name: Jiri
full_name: Holatko, Jiri
last_name: Holatko
- first_name: Anna
full_name: Gunina, Anna
id: '87876'
last_name: Gunina
- first_name: Gabrijel
full_name: Ondrasek, Gabrijel
last_name: Ondrasek
- first_name: Muhammad
full_name: Naveed, Muhammad
last_name: Naveed
- first_name: Tereza
full_name: Hammerschmiedt, Tereza
last_name: Hammerschmiedt
- first_name: Eliska
full_name: Kamenikova, Eliska
last_name: Kamenikova
- first_name: Saud
full_name: Alamri, Saud
last_name: Alamri
- first_name: Manzer H.
full_name: Siddiqui, Manzer H.
last_name: Siddiqui
- first_name: Antonin
full_name: Kintl, Antonin
last_name: Kintl
- first_name: Tivadar
full_name: Baltazar, Tivadar
last_name: Baltazar
- first_name: Ondrej
full_name: Malicek, Ondrej
last_name: Malicek
- first_name: Jiri
full_name: Kucerik, Jiri
last_name: Kucerik
citation:
ama: 'Brtnicky M, Mustafa A, Holatko J, et al. Soil texture-driven modulation of
poly-3-hydroxybutyrate (P3HB) biodegradation: Microbial shifts, and trade-offs
between nutrient availability and lettuce growth. Environmental Research.
2025;278. doi:10.1016/j.envres.2025.121618'
apa: 'Brtnicky, M., Mustafa, A., Holatko, J., Gunina, A., Ondrasek, G., Naveed,
M., Hammerschmiedt, T., Kamenikova, E., Alamri, S., Siddiqui, M. H., Kintl, A.,
Baltazar, T., Malicek, O., & Kucerik, J. (2025). Soil texture-driven modulation
of poly-3-hydroxybutyrate (P3HB) biodegradation: Microbial shifts, and trade-offs
between nutrient availability and lettuce growth. Environmental Research,
278, Article 121618. https://doi.org/10.1016/j.envres.2025.121618'
bjps: 'Brtnicky M et al. (2025) Soil Texture-Driven Modulation of
Poly-3-Hydroxybutyrate (P3HB) Biodegradation: Microbial Shifts, and Trade-Offs
between Nutrient Availability and Lettuce Growth. Environmental Research
278.'
chicago: 'Brtnicky, Martin, Adnan Mustafa, Jiri Holatko, Anna Gunina, Gabrijel Ondrasek,
Muhammad Naveed, Tereza Hammerschmiedt, et al. “Soil Texture-Driven Modulation
of Poly-3-Hydroxybutyrate (P3HB) Biodegradation: Microbial Shifts, and Trade-Offs
between Nutrient Availability and Lettuce Growth.” Environmental Research
278 (2025). https://doi.org/10.1016/j.envres.2025.121618.'
chicago-de: 'Brtnicky, Martin, Adnan Mustafa, Jiri Holatko, Anna Gunina, Gabrijel
Ondrasek, Muhammad Naveed, Tereza Hammerschmiedt, u. a. 2025. Soil texture-driven
modulation of poly-3-hydroxybutyrate (P3HB) biodegradation: Microbial shifts,
and trade-offs between nutrient availability and lettuce growth. Environmental
Research 278. doi:10.1016/j.envres.2025.121618,
.'
din1505-2-1: 'Brtnicky,
Martin ; Mustafa, Adnan ;
Holatko, Jiri ; Gunina,
Anna ; Ondrasek, Gabrijel
; Naveed, Muhammad ; Hammerschmiedt,
Tereza ; Kamenikova, Eliska
; u. a.: Soil texture-driven modulation of poly-3-hydroxybutyrate (P3HB)
biodegradation: Microbial shifts, and trade-offs between nutrient availability
and lettuce growth. In: Environmental Research Bd. 278. San Diego, Calif.,
Elsevier BV (2025)'
havard: 'M. Brtnicky, A. Mustafa, J. Holatko, A. Gunina, G. Ondrasek, M. Naveed,
T. Hammerschmiedt, E. Kamenikova, S. Alamri, M.H. Siddiqui, A. Kintl, T. Baltazar,
O. Malicek, J. Kucerik, Soil texture-driven modulation of poly-3-hydroxybutyrate
(P3HB) biodegradation: Microbial shifts, and trade-offs between nutrient availability
and lettuce growth, Environmental Research. 278 (2025).'
ieee: 'M. Brtnicky et al., “Soil texture-driven modulation of poly-3-hydroxybutyrate
(P3HB) biodegradation: Microbial shifts, and trade-offs between nutrient availability
and lettuce growth,” Environmental Research, vol. 278, Art. no. 121618,
2025, doi: 10.1016/j.envres.2025.121618.'
mla: 'Brtnicky, Martin, et al. “Soil Texture-Driven Modulation of Poly-3-Hydroxybutyrate
(P3HB) Biodegradation: Microbial Shifts, and Trade-Offs between Nutrient Availability
and Lettuce Growth.” Environmental Research, vol. 278, 121618, 2025, https://doi.org/10.1016/j.envres.2025.121618.'
short: M. Brtnicky, A. Mustafa, J. Holatko, A. Gunina, G. Ondrasek, M. Naveed, T.
Hammerschmiedt, E. Kamenikova, S. Alamri, M.H. Siddiqui, A. Kintl, T. Baltazar,
O. Malicek, J. Kucerik, Environmental Research 278 (2025).
ufg: 'Brtnicky, Martin u. a.: Soil texture-driven modulation of poly-3-hydroxybutyrate
(P3HB) biodegradation: Microbial shifts, and trade-offs between nutrient availability
and lettuce growth, in: Environmental Research 278 (2025).'
van: 'Brtnicky M, Mustafa A, Holatko J, Gunina A, Ondrasek G, Naveed M, et al. Soil
texture-driven modulation of poly-3-hydroxybutyrate (P3HB) biodegradation: Microbial
shifts, and trade-offs between nutrient availability and lettuce growth. Environmental
Research. 2025;278.'
date_created: 2025-05-08T08:07:20Z
date_updated: 2025-05-08T08:11:31Z
department:
- _id: DEP8000
doi: 10.1016/j.envres.2025.121618
external_id:
pmid:
- '40252798'
intvolume: ' 278'
keyword:
- Bioplastics
- Nutrient acquisition
- Plant growth reduction
- Soil microbes
- Soil texture.
language:
- iso: eng
place: San Diego, Calif.
pmid: '1'
publication: Environmental Research
publication_identifier:
eissn:
- 1096-0953
issn:
- 0013-9351
publication_status: published
publisher: Elsevier BV
quality_controlled: '1'
status: public
title: 'Soil texture-driven modulation of poly-3-hydroxybutyrate (P3HB) biodegradation:
Microbial shifts, and trade-offs between nutrient availability and lettuce growth'
type: scientific_journal_article
user_id: '83781'
volume: 278
year: '2025'
...
---
_id: '11284'
abstract:
- lang: eng
text: 'Introduction: Non-communicable diseases are the global disease burden of
our time, with physical inactivity identified as one major risk factor. Green
spaces are associated with increased physical activity of nearby residents. But
there are still gaps in understanding which proximity and what characteristics
of green spaces can trigger physical activity. This study aims to unveil these
differences with a rigorous sensitivity analysis. Methods: We gathered data on
self-reported health and physical activity from 1365 participants in selected
neighbourhoods in Porto, Nantes, Sofia, and Hoje-Taastrup. Spatial data were retrieved
from OpenStreetMap. We followed the PRIGSHARE guidelines to control for bias.
Around the residential addresses, we generated seven different green space indicators
for 15 distances (100-1500 m) using the AID-PRIGSHARE tool. We then analysed each
of these 105 green space indicators together with physical activity and health
in 105 adjusted structural equation models. Results: Green space accessibility
and green space uses indicators showed a pattern of significant positive associations
to physical activity and indirect to health at distances of 1100 m or less, with
a peak at 600 m for most indicators. Greenness in close proximity (100 m) had
significant positive effects on physical activity and indirect effects on health.
Surrounding greenness showed positive direct effects on health at 500-1100 m and
so do green corridors in 800 m network distance. In contrast, a high quantity
of green space uses, and surrounding greenness measured in a larger radius (1100-1500
m) showed a negative relationship with physical activity and indirect health effects.
Conclusions: Our results provide insight into how green space characteristics
can influence health at different scales, with important implications for urban
planners on how to integrate accessible green spaces into urban structures and
public health decision-makers on the ability of green spaces to combat physical
inactivity.'
article_number: '117605'
author:
- first_name: Marcel
full_name: Cardinali, Marcel
id: '58462'
last_name: Cardinali
orcid: 0000-0002-8841-9345
- first_name: Mariëlle A.
full_name: Beenackers, Mariëlle A.
last_name: Beenackers
- first_name: Arjan
full_name: van Timmeren, Arjan
last_name: van Timmeren
- first_name: Uta
full_name: Pottgiesser, Uta
id: '27166'
last_name: Pottgiesser
orcid: 0000-0002-8594-3168
citation:
ama: 'Cardinali M, Beenackers MA, van Timmeren A, Pottgiesser U. The relation between
proximity to and characteristics of green spaces to physical activity and health:
A multi-dimensional sensitivity analysis in four European cities. Environmental
research : a multidisziplinary journal of environmental sciences, ecology, and
public health . 2023;241. doi:10.1016/j.envres.2023.117605'
apa: 'Cardinali, M., Beenackers, M. A., van Timmeren, A., & Pottgiesser, U.
(2023). The relation between proximity to and characteristics of green spaces
to physical activity and health: A multi-dimensional sensitivity analysis in four
European cities. Environmental Research : A Multidisziplinary Journal of Environmental
Sciences, Ecology, and Public Health , 241, Article 117605. https://doi.org/10.1016/j.envres.2023.117605'
bjps: 'Cardinali M et al. (2023) The Relation between Proximity to
and Characteristics of Green Spaces to Physical Activity and Health: A Multi-Dimensional
Sensitivity Analysis in Four European Cities. Environmental research : a multidisziplinary
journal of environmental sciences, ecology, and public health 241.'
chicago: 'Cardinali, Marcel, Mariëlle A. Beenackers, Arjan van Timmeren, and Uta
Pottgiesser. “The Relation between Proximity to and Characteristics of Green Spaces
to Physical Activity and Health: A Multi-Dimensional Sensitivity Analysis in Four
European Cities.” Environmental Research : A Multidisziplinary Journal of Environmental
Sciences, Ecology, and Public Health 241 (2023). https://doi.org/10.1016/j.envres.2023.117605.'
chicago-de: 'Cardinali, Marcel, Mariëlle A. Beenackers, Arjan van Timmeren und Uta
Pottgiesser. 2023. The relation between proximity to and characteristics of green
spaces to physical activity and health: A multi-dimensional sensitivity analysis
in four European cities. Environmental research : a multidisziplinary journal
of environmental sciences, ecology, and public health 241. doi:10.1016/j.envres.2023.117605,
.'
din1505-2-1: 'Cardinali, Marcel ;
Beenackers, Mariëlle A. ; van Timmeren, Arjan ; Pottgiesser,
Uta: The relation between proximity to and characteristics of green spaces
to physical activity and health: A multi-dimensional sensitivity analysis in four
European cities. In: Environmental research : a multidisziplinary journal of
environmental sciences, ecology, and public health Bd. 241. San Diego, Calif.,
Elsevier BV (2023)'
havard: 'M. Cardinali, M.A. Beenackers, A. van Timmeren, U. Pottgiesser, The relation
between proximity to and characteristics of green spaces to physical activity
and health: A multi-dimensional sensitivity analysis in four European cities,
Environmental Research : A Multidisziplinary Journal of Environmental Sciences,
Ecology, and Public Health . 241 (2023).'
ieee: 'M. Cardinali, M. A. Beenackers, A. van Timmeren, and U. Pottgiesser, “The
relation between proximity to and characteristics of green spaces to physical
activity and health: A multi-dimensional sensitivity analysis in four European
cities,” Environmental research : a multidisziplinary journal of environmental
sciences, ecology, and public health , vol. 241, Art. no. 117605, 2023, doi:
10.1016/j.envres.2023.117605.'
mla: 'Cardinali, Marcel, et al. “The Relation between Proximity to and Characteristics
of Green Spaces to Physical Activity and Health: A Multi-Dimensional Sensitivity
Analysis in Four European Cities.” Environmental Research : A Multidisziplinary
Journal of Environmental Sciences, Ecology, and Public Health , vol. 241,
117605, 2023, https://doi.org/10.1016/j.envres.2023.117605.'
short: 'M. Cardinali, M.A. Beenackers, A. van Timmeren, U. Pottgiesser, Environmental
Research : A Multidisziplinary Journal of Environmental Sciences, Ecology, and
Public Health 241 (2023).'
ufg: 'Cardinali, Marcel u. a.: The relation between proximity to and characteristics
of green spaces to physical activity and health: A multi-dimensional sensitivity
analysis in four European cities, in: Environmental research : a multidisziplinary
journal of environmental sciences, ecology, and public health 241 (2023).'
van: 'Cardinali M, Beenackers MA, van Timmeren A, Pottgiesser U. The relation between
proximity to and characteristics of green spaces to physical activity and health:
A multi-dimensional sensitivity analysis in four European cities. Environmental
research : a multidisziplinary journal of environmental sciences, ecology, and
public health . 2023;241.'
date_created: 2024-03-24T11:20:21Z
date_updated: 2025-06-25T12:41:11Z
department:
- _id: DEP1000
- _id: DEP1055
doi: 10.1016/j.envres.2023.117605
external_id:
isi:
- '001121173400001'
pmid:
- '37956752'
intvolume: ' 241'
isi: '1'
keyword:
- Greenspace
- Mediator
- Behaviour
- Sedentary lifestyle
- Public health
language:
- iso: eng
place: San Diego, Calif.
pmid: '1'
publication: 'Environmental research : a multidisziplinary journal of environmental
sciences, ecology, and public health '
publication_identifier:
eissn:
- 1096-0953
issn:
- 0013-9351
publication_status: published
publisher: Elsevier BV
status: public
title: 'The relation between proximity to and characteristics of green spaces to physical
activity and health: A multi-dimensional sensitivity analysis in four European cities'
type: scientific_journal_article
user_id: '83781'
volume: 241
year: '2023'
...
---
_id: '13013'
abstract:
- lang: eng
text: 'The relationship between green spaces and health is attracting more and more
societal and research interest. The research field is however still suffering
from its differing monodisciplinary origins. Now in a multidisciplinary environment
on its way to a truly interdisciplinary field, there is a need for a common understanding,
precision in green space indicators, and coherent assessment of the complexity
of daily living environments. In several reviews, common protocols and open-source
scripts are considered a high priority to advance the field. Realizing these issues,
we developed PRIGSHARE (Preferred Reporting Items in Greenspace Health Research).
It is accompanied by an open-source script that supports non-spatial disciplines
in assessing greenness and green space on different scales and types. The PRIGSHARE
checklist contains 21 items that have been identified as a risk of bias and are
necessary for understanding and comparison of studies. The checklist is divided
into the following topics: objectives (3 items), scope (3 items), spatial assessment
(7 items), vegetation assessment (4 items), and context assessment (4 items).
For each item, we include a pathway-specific (if relevant) rationale and explanation.
The PRIGSHARE guiding principles should be helpful to support a high-quality assessment
and synchronize the studies in the field while acknowledging the diversity of
study designs.'
article_number: '115893'
author:
- first_name: Marcel
full_name: Cardinali, Marcel
id: '58462'
last_name: Cardinali
orcid: 0000-0002-8841-9345
- first_name: Mariëlle A.
full_name: Beenackers, Mariëlle A.
last_name: Beenackers
- first_name: Arjan
full_name: van Timmeren, Arjan
last_name: van Timmeren
- first_name: Uta
full_name: Pottgiesser, Uta
id: '27166'
last_name: Pottgiesser
orcid: 0000-0002-8594-3168
citation:
ama: Cardinali M, Beenackers MA, van Timmeren A, Pottgiesser U. Preferred reporting
items in green space health research. Guiding principles for an interdisciplinary
field. Environmental Research. 2023;228. doi:10.1016/j.envres.2023.115893
apa: Cardinali, M., Beenackers, M. A., van Timmeren, A., & Pottgiesser, U. (2023).
Preferred reporting items in green space health research. Guiding principles for
an interdisciplinary field. Environmental Research, 228, Article
115893. https://doi.org/10.1016/j.envres.2023.115893
bjps: Cardinali M et al. (2023) Preferred Reporting Items in Green
Space Health Research. Guiding Principles for an Interdisciplinary Field. Environmental
Research 228.
chicago: Cardinali, Marcel, Mariëlle A. Beenackers, Arjan van Timmeren, and Uta
Pottgiesser. “Preferred Reporting Items in Green Space Health Research. Guiding
Principles for an Interdisciplinary Field.” Environmental Research 228
(2023). https://doi.org/10.1016/j.envres.2023.115893.
chicago-de: Cardinali, Marcel, Mariëlle A. Beenackers, Arjan van Timmeren und Uta
Pottgiesser. 2023. Preferred reporting items in green space health research. Guiding
principles for an interdisciplinary field. Environmental Research 228.
doi:10.1016/j.envres.2023.115893,
.
din1505-2-1: 'Cardinali, Marcel ;
Beenackers, Mariëlle A. ; van Timmeren, Arjan ; Pottgiesser,
Uta: Preferred reporting items in green space health research. Guiding
principles for an interdisciplinary field. In: Environmental Research Bd.
228, Elsevier BV (2023)'
havard: M. Cardinali, M.A. Beenackers, A. van Timmeren, U. Pottgiesser, Preferred
reporting items in green space health research. Guiding principles for an interdisciplinary
field., Environmental Research. 228 (2023).
ieee: 'M. Cardinali, M. A. Beenackers, A. van Timmeren, and U. Pottgiesser, “Preferred
reporting items in green space health research. Guiding principles for an interdisciplinary
field.,” Environmental Research, vol. 228, Art. no. 115893, 2023, doi:
10.1016/j.envres.2023.115893.'
mla: Cardinali, Marcel, et al. “Preferred Reporting Items in Green Space Health
Research. Guiding Principles for an Interdisciplinary Field.” Environmental
Research, vol. 228, 115893, 2023, https://doi.org/10.1016/j.envres.2023.115893.
short: M. Cardinali, M.A. Beenackers, A. van Timmeren, U. Pottgiesser, Environmental
Research 228 (2023).
ufg: 'Cardinali, Marcel u. a.: Preferred reporting items in green space health
research. Guiding principles for an interdisciplinary field., in: Environmental
Research 228 (2023).'
van: Cardinali M, Beenackers MA, van Timmeren A, Pottgiesser U. Preferred reporting
items in green space health research. Guiding principles for an interdisciplinary
field. Environmental Research. 2023;228.
date_created: 2025-06-24T13:12:20Z
date_updated: 2025-06-25T13:14:05Z
department:
- _id: DEP1600
doi: 10.1016/j.envres.2023.115893
external_id:
isi:
- '000984485200001'
pmid:
- '37054830'
intvolume: ' 228'
isi: '1'
keyword:
- Greenspace
- Well-being
- Public health
- Pollution
- Behavior
- Stress
language:
- iso: eng
pmid: '1'
publication: Environmental Research
publication_identifier:
eissn:
- 1096-0953
issn:
- 0013-9351
publication_status: published
publisher: Elsevier BV
quality_controlled: '1'
status: public
title: Preferred reporting items in green space health research. Guiding principles
for an interdisciplinary field.
type: scientific_journal_article
user_id: '83781'
volume: 228
year: '2023'
...