---
_id: '12967'
abstract:
- lang: eng
  text: "Objectives\r\nTo evaluate matrix-assisted laser desorption ionisation time
    of flight mass spectrometry (MALDI-TOF MS) combined with the Sepsityper kit (Bruker
    Daltoniks GmbH, Bremen) for the direct detection of bacterial species from inoculated
    blood cultures from dogs and cats.\r\nMaterials and Methods\r\nCanine and feline
    blood samples were inoculated with typical sepsis-causing bacteria such as Staphylococcus
    intermedius, Staphylococcus aureus, Streptococcus canis, Enterococcus faecalis,
    Escherichia coli and Pseudomonas aeruginosa at two distinct concentrations (each
    in triplicate), resulting in 72 blood culture bottles incubated at 37°C. Samples
    were comparatively analysed with MALDI-TOF MS after preparation with the Sepsityper
    kit and also by standard bacteriology (culturing and biochemical characterisation).\r\nResults\r\nBacterial
    species identified from agar plates and by MALDI-TOF MS from blood culture bottles
    were identical for all samples. The MALDI Biotyper software (Bruker Daltoniks)
    correctly identified all bacterial strains from inoculated canine and feline blood
    with analysis indicating very good precision.\r\nClinical Significance\r\nMALDI-TOF
    MS analysis combined with the Sepsityper kit is a reliable tool for a quick detection
    of veterinary-relevant bacterial species directly from blood culture bottles.
    This approach could reduce the time for identification of critical species to
    only 24 hours."
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: C.
  full_name: Gottschalk, C.
  last_name: Gottschalk
- first_name: R. Kk
  full_name: Straubinger, R. Kk
  last_name: Straubinger
- first_name: K.
  full_name: Schwaiger, K.
  last_name: Schwaiger
- first_name: R.
  full_name: Dörfelt, R.
  last_name: Dörfelt
citation:
  ama: Ulrich S, Gottschalk C, Straubinger RK, Schwaiger K, Dörfelt R. Acceleration
    of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood.
    <i>Journal of Small Animal Practice</i>. 2019;61(1):42-45. doi:<a href="https://doi.org/10.1111/jsap.13056">10.1111/jsap.13056</a>
  apa: Ulrich, S., Gottschalk, C., Straubinger, R. K., Schwaiger, K., &#38; Dörfelt,
    R. (2019). Acceleration of the identification of sepsis‐inducing bacteria in cultures
    of dog and cat blood. <i>Journal of Small Animal Practice</i>, <i>61</i>(1), 42–45.
    <a href="https://doi.org/10.1111/jsap.13056">https://doi.org/10.1111/jsap.13056</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2019) Acceleration of the Identification of
    Sepsis‐inducing Bacteria in Cultures of Dog and Cat Blood. <i>Journal of Small
    Animal Practice</i> <b>61</b>, 42–45.
  chicago: 'Ulrich, Sebastian, C. Gottschalk, R. Kk Straubinger, K. Schwaiger, and
    R. Dörfelt. “Acceleration of the Identification of Sepsis‐inducing Bacteria in
    Cultures of Dog and Cat Blood.” <i>Journal of Small Animal Practice</i> 61, no.
    1 (2019): 42–45. <a href="https://doi.org/10.1111/jsap.13056">https://doi.org/10.1111/jsap.13056</a>.'
  chicago-de: 'Ulrich, Sebastian, C. Gottschalk, R. Kk Straubinger, K. Schwaiger und
    R. Dörfelt. 2019. Acceleration of the identification of sepsis‐inducing bacteria
    in cultures of dog and cat blood. <i>Journal of Small Animal Practice</i> 61,
    Nr. 1: 42–45. doi:<a href="https://doi.org/10.1111/jsap.13056">10.1111/jsap.13056</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Gottschalk, C.</span> ; <span style="font-variant:small-caps;">Straubinger,
    R. Kk</span> ; <span style="font-variant:small-caps;">Schwaiger, K.</span> ; <span
    style="font-variant:small-caps;">Dörfelt, R.</span>: Acceleration of the identification
    of sepsis‐inducing bacteria in cultures of dog and cat blood. In: <i>Journal of
    Small Animal Practice</i> Bd. 61. Oxford [u.a.], Wiley-Blackwell (2019), Nr. 1,
    S. 42–45'
  havard: S. Ulrich, C. Gottschalk, R.K. Straubinger, K. Schwaiger, R. Dörfelt, Acceleration
    of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood,
    Journal of Small Animal Practice. 61 (2019) 42–45.
  ieee: 'S. Ulrich, C. Gottschalk, R. K. Straubinger, K. Schwaiger, and R. Dörfelt,
    “Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog
    and cat blood,” <i>Journal of Small Animal Practice</i>, vol. 61, no. 1, pp. 42–45,
    2019, doi: <a href="https://doi.org/10.1111/jsap.13056">10.1111/jsap.13056</a>.'
  mla: Ulrich, Sebastian, et al. “Acceleration of the Identification of Sepsis‐inducing
    Bacteria in Cultures of Dog and Cat Blood.” <i>Journal of Small Animal Practice</i>,
    vol. 61, no. 1, 2019, pp. 42–45, <a href="https://doi.org/10.1111/jsap.13056">https://doi.org/10.1111/jsap.13056</a>.
  short: S. Ulrich, C. Gottschalk, R.K. Straubinger, K. Schwaiger, R. Dörfelt, Journal
    of Small Animal Practice 61 (2019) 42–45.
  ufg: '<b>Ulrich, Sebastian u. a.</b>: Acceleration of the identification of sepsis‐inducing
    bacteria in cultures of dog and cat blood, in: <i>Journal of Small Animal Practice</i>
    61 (2019), H. 1,  S. 42–45.'
  van: Ulrich S, Gottschalk C, Straubinger RK, Schwaiger K, Dörfelt R. Acceleration
    of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood.
    Journal of Small Animal Practice. 2019;61(1):42–5.
date_created: 2025-06-15T10:20:55Z
date_updated: 2025-06-18T11:24:12Z
department:
- _id: DEP4010
doi: 10.1111/jsap.13056
extern: '1'
intvolume: '        61'
issue: '1'
language:
- iso: eng
page: 42-45
place: Oxford [u.a.]
publication: Journal of Small Animal Practice
publication_identifier:
  issn:
  - 0022-4510
  - 1748-5827
publication_status: published
publisher: Wiley-Blackwell
quality_controlled: '1'
status: public
title: Acceleration of the identification of sepsis‐inducing bacteria in cultures
  of dog and cat blood
type: scientific_journal_article
user_id: '83781'
volume: 61
year: '2019'
...