[{"status":"public","department":[{"_id":"DEP4010"}],"intvolume":"        58","page":"1654-1664","user_id":"83781","place":"Oxford [u.a.] ","issue":"7","title":"Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany","citation":{"ama":"Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. <i>Transfusion</i>. 2018;58(7):1654-1664. doi:<a href=\"https://doi.org/10.1111/trf.14617\">10.1111/trf.14617</a>","ieee":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, and J. Dreier, “Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany,” <i>Transfusion</i>, vol. 58, no. 7, pp. 1654–1664, 2018, doi: <a href=\"https://doi.org/10.1111/trf.14617\">10.1111/trf.14617</a>.","apa":"Vollmer, T., Dabisch-Ruthe, M., Weinstock, M., Knabbe, C., &#38; Dreier, J. (2018). Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. <i>Transfusion</i>, <i>58</i>(7), 1654–1664. <a href=\"https://doi.org/10.1111/trf.14617\">https://doi.org/10.1111/trf.14617</a>","mla":"Vollmer, Tanja, et al. “Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany.” <i>Transfusion</i>, vol. 58, no. 7, 2018, pp. 1654–64, <a href=\"https://doi.org/10.1111/trf.14617\">https://doi.org/10.1111/trf.14617</a>.","ufg":"<b>Vollmer, Tanja u. a.</b>: Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany, in: <i>Transfusion</i> 58 (2018), H. 7,  S. 1654–1664.","short":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Transfusion 58 (2018) 1654–1664.","chicago-de":"Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe und Jens Dreier. 2018. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. <i>Transfusion</i> 58, Nr. 7: 1654–1664. doi:<a href=\"https://doi.org/10.1111/trf.14617\">10.1111/trf.14617</a>, .","chicago":"Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe, and Jens Dreier. “Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany.” <i>Transfusion</i> 58, no. 7 (2018): 1654–64. <a href=\"https://doi.org/10.1111/trf.14617\">https://doi.org/10.1111/trf.14617</a>.","bjps":"<b>Vollmer T <i>et al.</i></b> (2018) Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany. <i>Transfusion</i> <b>58</b>, 1654–1664.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Vollmer, Tanja</span> ; <span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Weinstock, Melanie</span> ; <span style=\"font-variant:small-caps;\">Knabbe, Cornelius</span> ; <span style=\"font-variant:small-caps;\">Dreier, Jens</span>: Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. In: <i>Transfusion</i> Bd. 58. Oxford [u.a.] , Wiley-Blackwell (2018), Nr. 7, S. 1654–1664","havard":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany, Transfusion. 58 (2018) 1654–1664.","van":"Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion. 2018;58(7):1654–64."},"doi":"10.1111/trf.14617","type":"scientific_journal_article","_id":"12988","quality_controlled":"1","year":"2018","language":[{"iso":"eng"}],"date_created":"2025-06-17T06:55:03Z","author":[{"full_name":"Vollmer, Tanja","first_name":"Tanja","last_name":"Vollmer"},{"first_name":"Mareike","id":"66516","full_name":"Dabisch-Ruthe, Mareike","orcid":"https://orcid.org/0009-0008-7644-0826","last_name":"Dabisch-Ruthe"},{"last_name":"Weinstock","full_name":"Weinstock, Melanie","first_name":"Melanie"},{"first_name":"Cornelius","full_name":"Knabbe, Cornelius","last_name":"Knabbe"},{"last_name":"Dreier","full_name":"Dreier, Jens","first_name":"Jens"}],"publication_status":"published","volume":58,"publication_identifier":{"eissn":["1537-2995"],"issn":["0041-1132"]},"abstract":[{"text":"BACKGROUND\r\nBacterial contamination of platelet concentrates (PCs) is still a major challenge in transfusion medicine. Different methodologic concepts and screening strategies have been developed and investigated concerning their usability. We evaluated the feasibility of BacT/ALERT automated culture (BacT/A, bioMérieux) with late sampling after 3 days at the earliest.\r\nSTUDY DESIGN AND METHODS\r\nTwenty-four bacterial strains isolated from PCs and six relevant strains from reference stocks were spiked into apheresis-derived PCs (10-60 colony-forming units [CFU]/bag). Sampling was performed after 3 days, and bacterial detection was investigated using the two detection methods (BacT/A and BactiFlow [BF], bioMérieux). The maximum time-to-result of BacT/A was set to less than 12 hours.\r\nRESULTS\r\nAll medium- or high-pathogenic strains are capable of proliferating to high titers, and 100% of contaminated samples were detected by BF and BacT/A (6 to ≤12 h incubation); lower detection rates of BacT/A were obtained within 6 hours of incubation (≤6 h: 76.2-93.4%). The majority of low-pathogenic isolates are also capable of growing in PCs (89.7%), showing a detection rate of 74.3% for BF versus 54.3% for BacT/A (6 to ≤12 h incubation). BacT/A failed to detect bacteria within 6 hours of incubation. Certainly, a small number of strains did not grow under PC storage conditions and were detectable by BacT/A only with increased detection times.\r\nCONCLUSIONS\r\nLate sampling after 3 days at the earliest, combined with reduced BacT/A incubation following the negative-to-date concept, offer an alternative opportunity to extend the shelf life of PCs from 4 to 5 days in Germany. The sensitivity of BacT/A with late sampling is nearly comparable to BF; the time-to-result is considerably longer.\r\n","lang":"eng"}],"publisher":"Wiley-Blackwell","publication":"Transfusion","date_updated":"2025-06-17T13:48:11Z"}]