---
_id: '12988'
abstract:
- lang: eng
text: "BACKGROUND\r\nBacterial contamination of platelet concentrates (PCs) is still
a major challenge in transfusion medicine. Different methodologic concepts and
screening strategies have been developed and investigated concerning their usability.
We evaluated the feasibility of BacT/ALERT automated culture (BacT/A, bioMérieux)
with late sampling after 3 days at the earliest.\r\nSTUDY DESIGN AND METHODS\r\nTwenty-four
bacterial strains isolated from PCs and six relevant strains from reference stocks
were spiked into apheresis-derived PCs (10-60 colony-forming units [CFU]/bag).
Sampling was performed after 3 days, and bacterial detection was investigated
using the two detection methods (BacT/A and BactiFlow [BF], bioMérieux). The maximum
time-to-result of BacT/A was set to less than 12 hours.\r\nRESULTS\r\nAll medium-
or high-pathogenic strains are capable of proliferating to high titers, and 100%
of contaminated samples were detected by BF and BacT/A (6 to ≤12 h incubation);
lower detection rates of BacT/A were obtained within 6 hours of incubation (≤6
h: 76.2-93.4%). The majority of low-pathogenic isolates are also capable of growing
in PCs (89.7%), showing a detection rate of 74.3% for BF versus 54.3% for BacT/A
(6 to ≤12 h incubation). BacT/A failed to detect bacteria within 6 hours of incubation.
Certainly, a small number of strains did not grow under PC storage conditions
and were detectable by BacT/A only with increased detection times.\r\nCONCLUSIONS\r\nLate
sampling after 3 days at the earliest, combined with reduced BacT/A incubation
following the negative-to-date concept, offer an alternative opportunity to extend
the shelf life of PCs from 4 to 5 days in Germany. The sensitivity of BacT/A with
late sampling is nearly comparable to BF; the time-to-result is considerably longer.\r\n"
author:
- first_name: Tanja
full_name: Vollmer, Tanja
last_name: Vollmer
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Melanie
full_name: Weinstock, Melanie
last_name: Weinstock
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Jens
full_name: Dreier, Jens
last_name: Dreier
citation:
ama: Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling
for automated culture to extend the platelet shelf life to 5 days in Germany.
Transfusion. 2018;58(7):1654-1664. doi:10.1111/trf.14617
apa: Vollmer, T., Dabisch-Ruthe, M., Weinstock, M., Knabbe, C., & Dreier, J.
(2018). Late sampling for automated culture to extend the platelet shelf life
to 5 days in Germany. Transfusion, 58(7), 1654–1664. https://doi.org/10.1111/trf.14617
bjps: Vollmer T et al. (2018) Late Sampling for Automated Culture
to Extend the Platelet Shelf Life to 5 Days in Germany. Transfusion 58,
1654–1664.
chicago: 'Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe,
and Jens Dreier. “Late Sampling for Automated Culture to Extend the Platelet Shelf
Life to 5 Days in Germany.” Transfusion 58, no. 7 (2018): 1654–64. https://doi.org/10.1111/trf.14617.'
chicago-de: 'Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius
Knabbe und Jens Dreier. 2018. Late sampling for automated culture to extend the
platelet shelf life to 5 days in Germany. Transfusion 58, Nr. 7: 1654–1664.
doi:10.1111/trf.14617, .'
din1505-2-1: 'Vollmer, Tanja ; Dabisch-Ruthe, Mareike ; Weinstock,
Melanie ; Knabbe, Cornelius
; Dreier, Jens: Late sampling for
automated culture to extend the platelet shelf life to 5 days in Germany. In:
Transfusion Bd. 58. Oxford [u.a.] , Wiley-Blackwell (2018), Nr. 7, S. 1654–1664'
havard: T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Late sampling
for automated culture to extend the platelet shelf life to 5 days in Germany,
Transfusion. 58 (2018) 1654–1664.
ieee: 'T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, and J. Dreier, “Late
sampling for automated culture to extend the platelet shelf life to 5 days in
Germany,” Transfusion, vol. 58, no. 7, pp. 1654–1664, 2018, doi: 10.1111/trf.14617.'
mla: Vollmer, Tanja, et al. “Late Sampling for Automated Culture to Extend the Platelet
Shelf Life to 5 Days in Germany.” Transfusion, vol. 58, no. 7, 2018, pp.
1654–64, https://doi.org/10.1111/trf.14617.
short: T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Transfusion
58 (2018) 1654–1664.
ufg: 'Vollmer, Tanja u. a.: Late sampling for automated culture to extend
the platelet shelf life to 5 days in Germany, in: Transfusion 58 (2018),
H. 7, S. 1654–1664.'
van: Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling
for automated culture to extend the platelet shelf life to 5 days in Germany.
Transfusion. 2018;58(7):1654–64.
date_created: 2025-06-17T06:55:03Z
date_updated: 2025-06-17T13:48:11Z
department:
- _id: DEP4010
doi: 10.1111/trf.14617
intvolume: ' 58'
issue: '7'
language:
- iso: eng
page: 1654-1664
place: 'Oxford [u.a.] '
publication: Transfusion
publication_identifier:
eissn:
- 1537-2995
issn:
- 0041-1132
publication_status: published
publisher: Wiley-Blackwell
quality_controlled: '1'
status: public
title: Late sampling for automated culture to extend the platelet shelf life to 5
days in Germany
type: scientific_journal_article
user_id: '83781'
volume: 58
year: '2018'
...