---
_id: '2397'
author:
- first_name: Tanja
  full_name: Hernández Rodríguez, Tanja
  last_name: Hernández Rodríguez
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
citation:
  ama: 'Hernández Rodríguez T, Frahm B. Design, Optimization and Adaptive Control
    of Cell Culture Seed Trains. In: Pörtner R, ed. <i>Animal Cell Biotechnology -
    Methods and Protocols</i>. Vol 2095. Methods in Molecular Biology. Berlin, Heidelberg,
    New York: Springer; 2020:251-267. doi:<a href="https://doi.org/10.1007/978-1-0716-0191-4_14">10.1007/978-1-0716-0191-4_14</a>'
  apa: 'Hernández Rodríguez, T., &#38; Frahm, B. (2020). Design, Optimization and
    Adaptive Control of Cell Culture Seed Trains. In R. Pörtner (Ed.), <i>Animal Cell
    Biotechnology - Methods and Protocols</i> (Vol. 2095, pp. 251–267). Berlin, Heidelberg,
    New York: Springer. <a href="https://doi.org/10.1007/978-1-0716-0191-4_14">https://doi.org/10.1007/978-1-0716-0191-4_14</a>'
  bjps: '<b>Hernández Rodríguez T and Frahm B</b> (2020) Design, Optimization and
    Adaptive Control of Cell Culture Seed Trains. In Pörtner R (ed.), <i>Animal Cell
    Biotechnology - Methods and Protocols</i>, vol. 2095. Berlin, Heidelberg, New
    York: Springer, pp. 251–267.'
  chicago: 'Hernández Rodríguez, Tanja, and Björn Frahm. “Design, Optimization and
    Adaptive Control of Cell Culture Seed Trains.” In <i>Animal Cell Biotechnology
    - Methods and Protocols</i>, edited by Ralf Pörtner, 2095:251–67. Methods in Molecular
    Biology. Berlin, Heidelberg, New York: Springer, 2020. <a href="https://doi.org/10.1007/978-1-0716-0191-4_14">https://doi.org/10.1007/978-1-0716-0191-4_14</a>.'
  chicago-de: 'Hernández Rodríguez, Tanja und Björn Frahm. 2020. Design, Optimization
    and Adaptive Control of Cell Culture Seed Trains. In: <i>Animal Cell Biotechnology
    - Methods and Protocols</i>, hg. von Ralf Pörtner, 2095:251–267. Methods in Molecular
    Biology. Berlin, Heidelberg, New York: Springer. doi:<a href="https://doi.org/10.1007/978-1-0716-0191-4_14,">10.1007/978-1-0716-0191-4_14,</a>
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Hernández Rodríguez, Tanja</span>
    ; <span style="font-variant:small-caps;">Frahm, Björn</span>: Design, Optimization
    and Adaptive Control of Cell Culture Seed Trains. In: <span style="font-variant:small-caps;">Pörtner,
    R.</span> (Hrsg.): <i>Animal Cell Biotechnology - Methods and Protocols</i>, <i>Methods
    in Molecular Biology</i>. Bd. 2095. Berlin, Heidelberg, New York : Springer, 2020,
    S. 251–267'
  havard: 'T. Hernández Rodríguez, B. Frahm, Design, Optimization and Adaptive Control
    of Cell Culture Seed Trains, in: R. Pörtner (Ed.), Animal Cell Biotechnology -
    Methods and Protocols, Springer, Berlin, Heidelberg, New York, 2020: pp. 251–267.'
  ieee: 'T. Hernández Rodríguez and B. Frahm, “Design, Optimization and Adaptive Control
    of Cell Culture Seed Trains,” in <i>Animal Cell Biotechnology - Methods and Protocols</i>,
    vol. 2095, R. Pörtner, Ed. Berlin, Heidelberg, New York: Springer, 2020, pp. 251–267.'
  mla: Hernández Rodríguez, Tanja, and Björn Frahm. “Design, Optimization and Adaptive
    Control of Cell Culture Seed Trains.” <i>Animal Cell Biotechnology - Methods and
    Protocols</i>, edited by Ralf Pörtner, vol. 2095, Springer, 2020, pp. 251–67,
    doi:<a href="https://doi.org/10.1007/978-1-0716-0191-4_14">10.1007/978-1-0716-0191-4_14</a>.
  short: 'T. Hernández Rodríguez, B. Frahm, in: R. Pörtner (Ed.), Animal Cell Biotechnology
    - Methods and Protocols, Springer, Berlin, Heidelberg, New York, 2020, pp. 251–267.'
  ufg: '<b>Hernández Rodríguez, Tanja/Frahm, Björn (2020)</b>: Design, Optimization
    and Adaptive Control of Cell Culture Seed Trains, in: Ralf Pörtner (Hg.): <i>Animal
    Cell Biotechnology - Methods and Protocols</i> (=<i>Methods in Molecular Biology
    2095</i>), Berlin, Heidelberg, New York, S. 251–267.'
  van: 'Hernández Rodríguez T, Frahm B. Design, Optimization and Adaptive Control
    of Cell Culture Seed Trains. In: Pörtner R, editor. Animal Cell Biotechnology
    - Methods and Protocols. Berlin, Heidelberg, New York: Springer; 2020. p. 251–67.
    (Methods in Molecular Biology; vol. 2095).'
date_created: 2020-05-19T15:11:00Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4021
doi: 10.1007/978-1-0716-0191-4_14
editor:
- first_name: Ralf
  full_name: Pörtner, Ralf
  last_name: Pörtner
intvolume: '      2095'
language:
- iso: eng
page: 251-267
place: Berlin, Heidelberg, New York
publication: Animal Cell Biotechnology - Methods and Protocols
publication_identifier:
  eisbn:
  - 978-1-07-160191-4
  isbn:
  - 978-1-07-160190-7
  issn:
  - 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
series_title: Methods in Molecular Biology
status: public
title: Design, Optimization and Adaptive Control of Cell Culture Seed Trains
type: book_chapter
user_id: '45666'
volume: 2095
year: 2020
...
---
_id: '2399'
author:
- first_name: S.
  full_name: Deppe, S.
  last_name: Deppe
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
- first_name: V. C.
  full_name: Hass, V. C.
  last_name: Hass
- first_name: T.
  full_name: Hernández Rodríguez, T.
  last_name: Hernández Rodríguez
- first_name: K. B.
  full_name: Kuchemüller, K. B.
  last_name: Kuchemüller
- first_name: J.
  full_name: Möller, J.
  last_name: Möller
- first_name: R.
  full_name: Pörtner, R.
  last_name: Pörtner
citation:
  ama: 'Deppe S, Frahm B, Hass VC, et al. Estimation of Process Model Parameters.
    In: Pörtner R, ed. <i>Animal Cell Biotechnology: Methods and Protocols</i>. Vol
    2095. Methods in Molecular Biology. Berlin ; Heidelberg ; New York: Springer;
    2020:213-234. doi:<a href="https://doi.org/10.1007/978-1-0716-0191-4_12">10.1007/978-1-0716-0191-4_12</a>'
  apa: 'Deppe, S., Frahm, B., Hass, V. C., Hernández Rodríguez, T., Kuchemüller, K.
    B., Möller, J., &#38; Pörtner, R. (2020). Estimation of Process Model Parameters.
    In R. Pörtner (Ed.), <i>Animal Cell Biotechnology: Methods and Protocols</i> (Vol.
    2095, pp. 213–234). Berlin ; Heidelberg ; New York: Springer. <a href="https://doi.org/10.1007/978-1-0716-0191-4_12">https://doi.org/10.1007/978-1-0716-0191-4_12</a>'
  bjps: '<b>Deppe S <i>et al.</i></b> (2020) Estimation of Process Model Parameters.
    In Pörtner Ralf (ed.), <i>Animal Cell Biotechnology: Methods and Protocols</i>,
    vol. 2095. Berlin ; Heidelberg ; New York: Springer, pp. 213–234.'
  chicago: 'Deppe, S., Björn Frahm, V. C. Hass, T. Hernández Rodríguez, K. B. Kuchemüller,
    J. Möller, and R. Pörtner. “Estimation of Process Model Parameters.” In <i>Animal
    Cell Biotechnology: Methods and Protocols</i>, edited by Ralf Pörtner, 2095:213–34.
    Methods in Molecular Biology. Berlin ; Heidelberg ; New York: Springer, 2020.
    <a href="https://doi.org/10.1007/978-1-0716-0191-4_12">https://doi.org/10.1007/978-1-0716-0191-4_12</a>.'
  chicago-de: 'Deppe, S., Björn Frahm, V. C. Hass, T. Hernández Rodríguez, K. B. Kuchemüller,
    J. Möller und R. Pörtner. 2020. Estimation of Process Model Parameters. In: <i>Animal
    Cell Biotechnology: Methods and Protocols</i>, hg. von Ralf Pörtner, 2095:213–234.
    Methods in Molecular Biology. Berlin ; Heidelberg ; New York: Springer. doi:<a
    href="https://doi.org/10.1007/978-1-0716-0191-4_12,">10.1007/978-1-0716-0191-4_12,</a>
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Deppe, S.</span> ; <span style="font-variant:small-caps;">Frahm,
    Björn</span> ; <span style="font-variant:small-caps;">Hass, V. C.</span> ; <span
    style="font-variant:small-caps;">Hernández Rodríguez, T.</span> ; <span style="font-variant:small-caps;">Kuchemüller,
    K. B.</span> ; <span style="font-variant:small-caps;">Möller, J.</span> ; <span
    style="font-variant:small-caps;">Pörtner, R.</span>: Estimation of Process Model
    Parameters. In: <span style="font-variant:small-caps;">Pörtner, R.</span> (Hrsg.):
    <i>Animal Cell Biotechnology: Methods and Protocols</i>, <i>Methods in Molecular
    Biology</i>. Bd. 2095. Berlin ; Heidelberg ; New York : Springer, 2020, S. 213–234'
  havard: 'S. Deppe, B. Frahm, V.C. Hass, T. Hernández Rodríguez, K.B. Kuchemüller,
    J. Möller, R. Pörtner, Estimation of Process Model Parameters, in: R. Pörtner
    (Ed.), Animal Cell Biotechnology: Methods and Protocols, Springer, Berlin ; Heidelberg ;
    New York, 2020: pp. 213–234.'
  ieee: 'S. Deppe <i>et al.</i>, “Estimation of Process Model Parameters,” in <i>Animal
    Cell Biotechnology: Methods and Protocols</i>, vol. 2095, R. Pörtner, Ed. Berlin ;
    Heidelberg ; New York: Springer, 2020, pp. 213–234.'
  mla: 'Deppe, S., et al. “Estimation of Process Model Parameters.” <i>Animal Cell
    Biotechnology: Methods and Protocols</i>, edited by Ralf Pörtner, vol. 2095, Springer,
    2020, pp. 213–34, doi:<a href="https://doi.org/10.1007/978-1-0716-0191-4_12">10.1007/978-1-0716-0191-4_12</a>.'
  short: 'S. Deppe, B. Frahm, V.C. Hass, T. Hernández Rodríguez, K.B. Kuchemüller,
    J. Möller, R. Pörtner, in: R. Pörtner (Ed.), Animal Cell Biotechnology: Methods
    and Protocols, Springer, Berlin ; Heidelberg ; New York, 2020, pp. 213–234.'
  ufg: '<b>Deppe, S. et. al. (2020)</b>: Estimation of Process Model Parameters, in:
    Ralf Pörtner (Hg.): <i>Animal Cell Biotechnology: Methods and Protocols</i> (=<i>Methods
    in Molecular Biology 2095</i>), Berlin ; Heidelberg ; New York, S. 213–234.'
  van: 'Deppe S, Frahm B, Hass VC, Hernández Rodríguez T, Kuchemüller KB, Möller J,
    et al. Estimation of Process Model Parameters. In: Pörtner R, editor. Animal Cell
    Biotechnology: Methods and Protocols. Berlin ; Heidelberg ; New York: Springer;
    2020. p. 213–34. (Methods in Molecular Biology; vol. 2095).'
date_created: 2020-05-19T15:11:00Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4021
doi: 10.1007/978-1-0716-0191-4_12
editor:
- first_name: Ralf
  full_name: Pörtner, Ralf
  last_name: Pörtner
intvolume: '      2095'
language:
- iso: eng
page: 213-234
place: Berlin ; Heidelberg ; New York
publication: 'Animal Cell Biotechnology: Methods and Protocols'
publication_identifier:
  eisbn:
  - 978-1-07-160191-4
  isbn:
  - 978-1-07-160190-7
  issn:
  - 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
series_title: Methods in Molecular Biology
status: public
title: Estimation of Process Model Parameters
type: book_chapter
user_id: '45666'
volume: 2095
year: 2020
...
---
_id: '2394'
abstract:
- lang: eng
  text: For the production of biopharmaceuticals a seed train is required to generate
    an adequate number of cells for inoculation of the production bioreactor. This
    seed train is time- and cost-intensive but offers potential for optimization.
    A method and a protocol are described for the seed train mapping, directed modeling
    without major effort, and its optimization regarding selected optimization criteria
    such as optimal points in time for cell passaging. Furthermore, the method can
    also be applied for the set-up of a new seed train, for example for a new cell
    line. Although the chapter is directed towards suspension cell lines, the method
    is also generally applicable, e.g. for adherent cell lines.
author:
- first_name: Björn
  full_name: Frahm, Björn
  last_name: Frahm
citation:
  ama: 'Frahm B. Seed Train Optimization for Cell Culture. In: <i>Animal Cell Biotechnology</i>.
    Vol 1104. Methods in Molecular Biology . Totowa, NJ: Humana Press; 2013:355-367.
    doi:<a href="https://doi.org/10.1007/978-1-62703-733-4_22">10.1007/978-1-62703-733-4_22</a>'
  apa: 'Frahm, B. (2013). Seed Train Optimization for Cell Culture. In <i>Animal Cell
    Biotechnology</i> (Vol. 1104, pp. 355–367). Totowa, NJ: Humana Press. <a href="https://doi.org/10.1007/978-1-62703-733-4_22">https://doi.org/10.1007/978-1-62703-733-4_22</a>'
  bjps: '<b>Frahm B</b> (2013) Seed Train Optimization for Cell Culture. <i>Animal
    Cell Biotechnology</i>, vol. 1104. Totowa, NJ: Humana Press, pp. 355–367.'
  chicago: 'Frahm, Björn. “Seed Train Optimization for Cell Culture.” In <i>Animal
    Cell Biotechnology</i>, 1104:355–67. Methods in Molecular Biology . Totowa, NJ:
    Humana Press, 2013. <a href="https://doi.org/10.1007/978-1-62703-733-4_22">https://doi.org/10.1007/978-1-62703-733-4_22</a>.'
  chicago-de: 'Frahm, Björn. 2013. Seed Train Optimization for Cell Culture. In: <i>Animal
    Cell Biotechnology</i>, 1104:355–367. Methods in Molecular Biology . Totowa, NJ:
    Humana Press. doi:<a href="https://doi.org/10.1007/978-1-62703-733-4_22,">10.1007/978-1-62703-733-4_22,</a>
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Frahm, Björn</span>: Seed Train
    Optimization for Cell Culture. In: <i>Animal Cell Biotechnology</i>, <i>Methods
    in Molecular Biology </i>. Bd. 1104. Totowa, NJ : Humana Press, 2013, S. 355–367'
  havard: 'B. Frahm, Seed Train Optimization for Cell Culture, in: Animal Cell Biotechnology,
    Humana Press, Totowa, NJ, 2013: pp. 355–367.'
  ieee: 'B. Frahm, “Seed Train Optimization for Cell Culture,” in <i>Animal Cell Biotechnology</i>,
    vol. 1104, Totowa, NJ: Humana Press, 2013, pp. 355–367.'
  mla: Frahm, Björn. “Seed Train Optimization for Cell Culture.” <i>Animal Cell Biotechnology</i>,
    vol. 1104, Humana Press, 2013, pp. 355–67, doi:<a href="https://doi.org/10.1007/978-1-62703-733-4_22">10.1007/978-1-62703-733-4_22</a>.
  short: 'B. Frahm, in: Animal Cell Biotechnology, Humana Press, Totowa, NJ, 2013,
    pp. 355–367.'
  ufg: '<b>Frahm, Björn (2013)</b>: Seed Train Optimization for Cell Culture, in:
    <i>Animal Cell Biotechnology</i> (=<i>Methods in Molecular Biology  1104</i>),
    Totowa, NJ, S. 355–367.'
  van: 'Frahm B. Seed Train Optimization for Cell Culture. In: Animal Cell Biotechnology.
    Totowa, NJ: Humana Press; 2013. p. 355–67. (Methods in Molecular Biology ; vol.
    1104).'
date_created: 2020-05-19T14:45:43Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4021
doi: 10.1007/978-1-62703-733-4_22
intvolume: '      1104'
keyword:
- Seed train Optimization Modeling Prediction Space-Time-Yield (STY) Systems approach
  Bioinformatics Computational biotechnology Suspension Production
language:
- iso: eng
page: 355-367
place: Totowa, NJ
publication: Animal Cell Biotechnology
publication_identifier:
  eisbn:
  - '9781627037334'
  isbn:
  - '9781627037327'
  issn:
  - 1064-3745
  - 1940-6029
publication_status: published
publisher: Humana Press
series_title: 'Methods in Molecular Biology '
status: public
title: Seed Train Optimization for Cell Culture
type: book_chapter
user_id: '74004'
volume: 1104
year: 2013
...
---
_id: '10214'
abstract:
- lang: eng
  text: For the production of biopharmaceuticals a seed train is required to generate
    an adequate number of cells for inoculation of the production bioreactor. This
    seed train is time- and cost-intensive but offers potential for optimization.
    A method and a protocol are described for the seed train mapping, directed modeling
    without major effort, and its optimization regarding selected optimization criteria
    such as optimal points in time for cell passaging. Furthermore, the method can
    also be applied for the set-up of a new seed train, for example for a new cell
    line. Although the chapter is directed towards suspension cell lines, the method
    is also generally applicable, e.g. for adherent cell lines.
author:
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
citation:
  ama: 'Frahm B. Seed Train Optimization for Cell Culture. In: Pörtner R, ed. <i>Animal
    Cell Biotechnology - Methods and Protocols</i>. Vol 1104. 3rd ed. Methods in Molecular
    Biology. Humana Press; 2013:355-367. doi:<a href="https://doi.org/10.1007/978-1-62703-733-4_22">10.1007/978-1-62703-733-4_22</a>'
  apa: Frahm, B. (2013). Seed Train Optimization for Cell Culture. In R. Pörtner (Ed.),
    <i>Animal Cell Biotechnology - Methods and Protocols</i> (3rd ed., Vol. 1104,
    pp. 355–367). Humana Press. <a href="https://doi.org/10.1007/978-1-62703-733-4_22">https://doi.org/10.1007/978-1-62703-733-4_22</a>
  bjps: '<b>Frahm B</b> (2013) Seed Train Optimization for Cell Culture. In Pörtner
    R (ed.), <i>Animal Cell Biotechnology - Methods and Protocols</i>, 3rd ed., vol.
    1104. Totowa, NJ: Humana Press, pp. 355–367.'
  chicago: 'Frahm, Björn. “Seed Train Optimization for Cell Culture.” In <i>Animal
    Cell Biotechnology - Methods and Protocols</i>, edited by Ralf Pörtner, 3rd ed.,
    1104:355–67. Methods in Molecular Biology. Totowa, NJ: Humana Press, 2013. <a
    href="https://doi.org/10.1007/978-1-62703-733-4_22">https://doi.org/10.1007/978-1-62703-733-4_22</a>.'
  chicago-de: 'Frahm, Björn. 2013. Seed Train Optimization for Cell Culture. In: <i>Animal
    Cell Biotechnology - Methods and Protocols</i>, hg. von Ralf Pörtner, 1104:355–367.
    3. Aufl. Methods in Molecular Biology. Totowa, NJ: Humana Press. doi:<a href="https://doi.org/10.1007/978-1-62703-733-4_22">10.1007/978-1-62703-733-4_22</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Frahm, Björn</span>: Seed Train
    Optimization for Cell Culture. In: <span style="font-variant:small-caps;">Pörtner,
    R.</span> (Hrsg.): <i>Animal Cell Biotechnology - Methods and Protocols</i>, <i>Methods
    in Molecular Biology</i>. Bd. 1104. 3. Aufl. Totowa, NJ : Humana Press, 2013,
    S. 355–367'
  havard: 'B. Frahm, Seed Train Optimization for Cell Culture, in: R. Pörtner (Ed.),
    Animal Cell Biotechnology - Methods and Protocols, 3rd ed., Humana Press, Totowa,
    NJ, 2013: pp. 355–367.'
  ieee: 'B. Frahm, “Seed Train Optimization for Cell Culture,” in <i>Animal Cell Biotechnology
    - Methods and Protocols</i>, 3rd ed., vol. 1104, R. Pörtner, Ed. Totowa, NJ: Humana
    Press, 2013, pp. 355–367. doi: <a href="https://doi.org/10.1007/978-1-62703-733-4_22">10.1007/978-1-62703-733-4_22</a>.'
  mla: Frahm, Björn. “Seed Train Optimization for Cell Culture.” <i>Animal Cell Biotechnology
    - Methods and Protocols</i>, edited by Ralf Pörtner, 3rd ed., vol. 1104, Humana
    Press, 2013, pp. 355–67, <a href="https://doi.org/10.1007/978-1-62703-733-4_22">https://doi.org/10.1007/978-1-62703-733-4_22</a>.
  short: 'B. Frahm, in: R. Pörtner (Ed.), Animal Cell Biotechnology - Methods and
    Protocols, 3rd ed., Humana Press, Totowa, NJ, 2013, pp. 355–367.'
  ufg: '<b>Frahm, Björn</b>: Seed Train Optimization for Cell Culture, in: <i>Pörtner,
    Ralf (Hg.)</i>: Animal Cell Biotechnology - Methods and Protocols, Bd. 1104, Totowa,
    NJ <sup>3</sup>2013 (Methods in Molecular Biology),  S. 355–367.'
  van: 'Frahm B. Seed Train Optimization for Cell Culture. In: Pörtner R, editor.
    Animal Cell Biotechnology - Methods and Protocols. 3rd ed. Totowa, NJ: Humana
    Press; 2013. p. 355–67. (Methods in Molecular Biology; vol. 1104).'
date_created: 2023-08-14T17:50:56Z
date_updated: 2023-08-16T06:41:21Z
department:
- _id: DEP4000
doi: 10.1007/978-1-62703-733-4_22
edition: '3'
editor:
- first_name: Ralf
  full_name: Pörtner, Ralf
  last_name: Pörtner
intvolume: '      1104'
keyword:
- Seed train
- Optimization
- Modeling
- Prediction
- Space-Time-Yield (STY)
- Systems approach
- Bioinformatics
- Computational biotechnology
- Suspension
- Production
language:
- iso: eng
page: 355–367
place: Totowa, NJ
publication: Animal Cell Biotechnology - Methods and Protocols
publication_identifier:
  eisbn:
  - 978-1-62703-733-4
  eissn:
  - 1940-6029
  isbn:
  - 978-1-62703-732-7
  issn:
  - 1064-3745
publication_status: published
publisher: Humana Press
series_title: Methods in Molecular Biology
status: public
title: Seed Train Optimization for Cell Culture
type: book_chapter
user_id: '83781'
volume: 1104
year: '2013'
...