---
_id: '12950'
abstract:
- lang: eng
  text: The composition of the microbiome is subject to the host’s diet. In commercial
    laboratory mouse diets, different physical forms of the same diets are available,
    containing—according to their labels—identical ingredients and nutrient compositions.
    However, variations in nutrient composition and starch gelatinization due to production
    processes and their impact on digestibility have been described. In this study,
    a total of 48 C57BL/J6 mice were assigned to two equal groups and were fed diets
    (produced with different processes—extruded vs. pelleted) for eight weeks in two
    biological replicates. At the end of the experiment, samples were collected from
    five different gastrointestinal regions, including the stomach, small intestine,
    cecum, large intestine, and an extracorporeal region (feces), and the microbiome
    was analyzed with 16S rRNA gene amplicon sequencing. The replicates in both experiments
    differed significantly in their relative abundances of Muribaculaceae species.
    Furthermore, the gastrointestinal content of pellet-fed mice contained larger
    numbers of Lactobacillus species. These results indicate that starch gelatinization
    and ingredient composition significantly influence microbial makeup. In conclusion,
    different feed processing methods may affect fundamental digestive and metabolic
    processes, impacting animal experiments and biasing microbiome data.
article_number: '862'
author:
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Linda F.
  full_name: Böswald, Linda F.
  last_name: Böswald
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Ellen
  full_name: Kienzle, Ellen
  last_name: Kienzle
- first_name: Klaus
  full_name: Neuhaus, Klaus
  last_name: Neuhaus
- first_name: Ilias
  full_name: Lagkouvardos, Ilias
  last_name: Lagkouvardos
- first_name: Christian
  full_name: Zenner, Christian
  last_name: Zenner
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: Wenderlein J, Böswald LF, Ulrich S, et al. Processing Matters in Nutrient-Matched
    Laboratory Diets for Mice—Microbiome. <i>Animals</i>. 2021;11(3). doi:<a href="https://doi.org/10.3390/ani11030862">10.3390/ani11030862</a>
  apa: Wenderlein, J., Böswald, L. F., Ulrich, S., Kienzle, E., Neuhaus, K., Lagkouvardos,
    I., Zenner, C., &#38; Straubinger, R. K. (2021). Processing Matters in Nutrient-Matched
    Laboratory Diets for Mice—Microbiome. <i>Animals</i>, <i>11</i>(3), Article 862.
    <a href="https://doi.org/10.3390/ani11030862">https://doi.org/10.3390/ani11030862</a>
  bjps: <b>Wenderlein J <i>et al.</i></b> (2021) Processing Matters in Nutrient-Matched
    Laboratory Diets for Mice—Microbiome. <i>Animals</i> <b>11</b>.
  chicago: Wenderlein, Jasmin, Linda F. Böswald, Sebastian Ulrich, Ellen Kienzle,
    Klaus Neuhaus, Ilias Lagkouvardos, Christian Zenner, and Reinhard K. Straubinger.
    “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome.”
    <i>Animals</i> 11, no. 3 (2021). <a href="https://doi.org/10.3390/ani11030862">https://doi.org/10.3390/ani11030862</a>.
  chicago-de: Wenderlein, Jasmin, Linda F. Böswald, Sebastian Ulrich, Ellen Kienzle,
    Klaus Neuhaus, Ilias Lagkouvardos, Christian Zenner und Reinhard K. Straubinger.
    2021. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome.
    <i>Animals</i> 11, Nr. 3. doi:<a href="https://doi.org/10.3390/ani11030862">10.3390/ani11030862</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Wenderlein, Jasmin</span> ;
    <span style="font-variant:small-caps;">Böswald, Linda F.</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Kienzle, Ellen</span>
    ; <span style="font-variant:small-caps;">Neuhaus, Klaus</span> ; <span style="font-variant:small-caps;">Lagkouvardos,
    Ilias</span> ; <span style="font-variant:small-caps;">Zenner, Christian</span>
    ; <span style="font-variant:small-caps;">Straubinger, Reinhard K.</span>: Processing
    Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome. In: <i>Animals</i>
    Bd. 11. Basel, MDPI AG (2021), Nr. 3'
  havard: J. Wenderlein, L.F. Böswald, S. Ulrich, E. Kienzle, K. Neuhaus, I. Lagkouvardos,
    C. Zenner, R.K. Straubinger, Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Microbiome, Animals. 11 (2021).
  ieee: 'J. Wenderlein <i>et al.</i>, “Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Microbiome,” <i>Animals</i>, vol. 11, no. 3, Art. no. 862, 2021,
    doi: <a href="https://doi.org/10.3390/ani11030862">10.3390/ani11030862</a>.'
  mla: Wenderlein, Jasmin, et al. “Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Microbiome.” <i>Animals</i>, vol. 11, no. 3, 862, 2021, <a href="https://doi.org/10.3390/ani11030862">https://doi.org/10.3390/ani11030862</a>.
  short: J. Wenderlein, L.F. Böswald, S. Ulrich, E. Kienzle, K. Neuhaus, I. Lagkouvardos,
    C. Zenner, R.K. Straubinger, Animals 11 (2021).
  ufg: '<b>Wenderlein, Jasmin u. a.</b>: Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Microbiome, in: <i>Animals</i> 11 (2021), H. 3.'
  van: Wenderlein J, Böswald LF, Ulrich S, Kienzle E, Neuhaus K, Lagkouvardos I, et
    al. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome.
    Animals. 2021;11(3).
date_created: 2025-06-15T09:58:59Z
date_updated: 2025-06-16T12:07:21Z
department:
- _id: DEP4010
doi: 10.3390/ani11030862
extern: '1'
intvolume: '        11'
issue: '3'
keyword:
- feed processing
- starch gelatinization
- laboratory mouse
- diet
- intestinal microbiome
language:
- iso: eng
place: Basel
publication: Animals
publication_identifier:
  issn:
  - 2076-2615
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2021'
...