---
_id: '12973'
abstract:
- lang: eng
  text: 'Psychrophilic and psychrotolerant clostridia (n = 110) were isolated from
    vacuum-packed meat (beef and lamb), fresh venison and from skin and fecal samples
    of wild boars. They were identified to species level using MALDI-TOF MS, sequence
    and phylogeny analysis of the 16S rRNA and species specific multiplex qPCR. The
    results of all three methods were concordant. The majority of isolates were identified
    as C. tagluense-like Group I (n = 34) and Group II (n = 42). Thirty-five isolates
    could be identified to species level as follows: C. estertheticum (n = 15), C.
    frigoriphilum (n = 13), C. frigidicarnis (n = 1) and C. bowmanii (n = 5). This
    is the first report of detection and identification of C. frigoriphilum and C.
    tagluense-like Group II as causative agents of blown pack spoilage of beef. The
    species specific multiplex qPCR developed in this study could be applied to identify
    and to quantify the Clostridium species described above in suspicious meat juice
    samples.'
author:
- first_name: Samart
  full_name: Dorn-In, Samart
  last_name: Dorn-In
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Claudia
  full_name: Springer, Claudia
  last_name: Springer
- first_name: Leonard
  full_name: Barta, Leonard
  last_name: Barta
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
citation:
  ama: Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development
    of a multiplex qPCR for the species identification of Clostridium estertheticum,
    C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
    meats and from wild boars. <i>International Journal of Food Microbiology</i>.
    2018;286:162-169. doi:<a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">10.1016/j.ijfoodmicro.2018.08.020</a>
  apa: Dorn-In, S., Schwaiger, K., Springer, C., Barta, L., Ulrich, S., &#38; Gareis,
    M. (2018). Development of a multiplex qPCR for the species identification of Clostridium
    estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown
    pack spoilage (BPS) meats and from wild boars. <i>International Journal of Food
    Microbiology</i>, <i>286</i>, 162–169. <a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">https://doi.org/10.1016/j.ijfoodmicro.2018.08.020</a>
  bjps: <b>Dorn-In S <i>et al.</i></b> (2018) Development of a Multiplex QPCR for
    the Species Identification of Clostridium Estertheticum, C. Frigoriphilum, C.
    Bowmanii and C. Tagluense-like from Blown Pack Spoilage (BPS) Meats and from Wild
    Boars. <i>International Journal of Food Microbiology</i> <b>286</b>, 162–169.
  chicago: 'Dorn-In, Samart, Karin Schwaiger, Claudia Springer, Leonard Barta, Sebastian
    Ulrich, and Manfred Gareis. “Development of a Multiplex QPCR for the Species Identification
    of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like
    from Blown Pack Spoilage (BPS) Meats and from Wild Boars.” <i>International Journal
    of Food Microbiology</i> 286 (2018): 162–69. <a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">https://doi.org/10.1016/j.ijfoodmicro.2018.08.020</a>.'
  chicago-de: 'Dorn-In, Samart, Karin Schwaiger, Claudia Springer, Leonard Barta,
    Sebastian Ulrich und Manfred Gareis. 2018. Development of a multiplex qPCR for
    the species identification of Clostridium estertheticum, C. frigoriphilum, C.
    bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild
    boars. <i>International Journal of Food Microbiology</i> 286: 162–169. doi:<a
    href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">10.1016/j.ijfoodmicro.2018.08.020</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Dorn-In, Samart</span> ; <span
    style="font-variant:small-caps;">Schwaiger, Karin</span> ; <span style="font-variant:small-caps;">Springer,
    Claudia</span> ; <span style="font-variant:small-caps;">Barta, Leonard</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Gareis,
    Manfred</span>: Development of a multiplex qPCR for the species identification
    of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like
    from blown pack spoilage (BPS) meats and from wild boars. In: <i>International
    Journal of Food Microbiology</i> Bd. 286. Amsterdam, Elsevier  (2018), S. 162–169'
  havard: S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, M. Gareis, Development
    of a multiplex qPCR for the species identification of Clostridium estertheticum,
    C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
    meats and from wild boars, International Journal of Food Microbiology. 286 (2018)
    162–169.
  ieee: 'S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, and M. Gareis,
    “Development of a multiplex qPCR for the species identification of Clostridium
    estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown
    pack spoilage (BPS) meats and from wild boars,” <i>International Journal of Food
    Microbiology</i>, vol. 286, pp. 162–169, 2018, doi: <a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">10.1016/j.ijfoodmicro.2018.08.020</a>.'
  mla: Dorn-In, Samart, et al. “Development of a Multiplex QPCR for the Species Identification
    of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like
    from Blown Pack Spoilage (BPS) Meats and from Wild Boars.” <i>International Journal
    of Food Microbiology</i>, vol. 286, 2018, pp. 162–69, <a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">https://doi.org/10.1016/j.ijfoodmicro.2018.08.020</a>.
  short: S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, M. Gareis, International
    Journal of Food Microbiology 286 (2018) 162–169.
  ufg: '<b>Dorn-In, Samart u. a.</b>: Development of a multiplex qPCR for the species
    identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and
    C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars, in:
    <i>International Journal of Food Microbiology</i> 286 (2018),  S. 162–169.'
  van: Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development
    of a multiplex qPCR for the species identification of Clostridium estertheticum,
    C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
    meats and from wild boars. International Journal of Food Microbiology. 2018;286:162–9.
date_created: 2025-06-15T10:25:24Z
date_updated: 2025-06-18T11:27:12Z
department:
- _id: DEP4010
doi: 10.1016/j.ijfoodmicro.2018.08.020
extern: '1'
intvolume: '       286'
keyword:
- Blown pack spoilage
- MALDI-TOF MS
- PCR
- 16S rRNA
- C. frigoriphilum
- C. tagluense-like
language:
- iso: eng
page: 162-169
place: Amsterdam
publication: International Journal of Food Microbiology
publication_identifier:
  eissn:
  - 1879-3460
  issn:
  - 0168-1605
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Development of a multiplex qPCR for the species identification of Clostridium
  estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack
  spoilage (BPS) meats and from wild boars
type: scientific_journal_article
user_id: '83781'
volume: 286
year: '2018'
...