---
_id: '12965'
abstract:
- lang: eng
  text: "The Bacillus (B.) cereus group consists of nine recognized species which
    are present worldwide. B. cereus play an important role in food-borne diseases
    by producing different toxins. Yet, only a small percentage of B. cereus strains
    are able to produce the heat stable cereulide, the causative agent of emetic food
    poisoning. To minimize the entry of emetic B. cereus into the food chain, food
    business operators are dependent on efficient and reliable methods enabling the
    differentiation between emetic and non-emetic strains.\r\nCurrently, only time-consuming
    cell bioassays, molecular methods and tandem mass spectrometry are available for
    this purpose. Thus, the aim of the present study was to establish a fast and reliable
    method for the differentiation between emetic/non-emetic strains by MALDI-TOF
    MS. Selected strains/isolates of the B. cereus group as well as other Bacillus
    spp. (total n = 121) were cultured on sheep blood agar for 48 h before analysis.\r\nSubsequently,
    the cultures were directly analyzed by MALDI-TOF MS without prior extraction steps.
    The samples were measured in the mass range of m/z 800–1800 Da. Using ClinProTools
    3.0 statistical software and Flex analysis software (Bruker Daltonics GmbH, Bremen,
    Germany), a differentiation between emetic/non-emetic isolates was possible with
    a rate of correct identification of 99.1% by means of the evaluation of two specific
    biomarkers (m/z 1171 and 1187 Da)."
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Richard
  full_name: Dietrich, Richard
  last_name: Dietrich
- first_name: Erwin
  full_name: Märtlbauer, Erwin
  last_name: Märtlbauer
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
citation:
  ama: Ulrich S, Gottschalk C, Dietrich R, Märtlbauer E, Gareis M. Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS. <i>Food Microbiology</i>.
    2019;82:75-81. doi:<a href="https://doi.org/10.1016/j.fm.2019.01.012">10.1016/j.fm.2019.01.012</a>
  apa: Ulrich, S., Gottschalk, C., Dietrich, R., Märtlbauer, E., &#38; Gareis, M.
    (2019). Identification of cereulide producing Bacillus cereus by MALDI-TOF MS.
    <i>Food Microbiology</i>, <i>82</i>, 75–81. <a href="https://doi.org/10.1016/j.fm.2019.01.012">https://doi.org/10.1016/j.fm.2019.01.012</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2019) Identification of Cereulide Producing
    Bacillus Cereus by MALDI-TOF MS. <i>Food Microbiology</i> <b>82</b>, 75–81.
  chicago: 'Ulrich, Sebastian, Christoph Gottschalk, Richard Dietrich, Erwin Märtlbauer,
    and Manfred Gareis. “Identification of Cereulide Producing Bacillus Cereus by
    MALDI-TOF MS.” <i>Food Microbiology</i> 82 (2019): 75–81. <a href="https://doi.org/10.1016/j.fm.2019.01.012">https://doi.org/10.1016/j.fm.2019.01.012</a>.'
  chicago-de: 'Ulrich, Sebastian, Christoph Gottschalk, Richard Dietrich, Erwin Märtlbauer
    und Manfred Gareis. 2019. Identification of cereulide producing Bacillus cereus
    by MALDI-TOF MS. <i>Food Microbiology</i> 82: 75–81. doi:<a href="https://doi.org/10.1016/j.fm.2019.01.012">10.1016/j.fm.2019.01.012</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Gottschalk, Christoph</span> ; <span style="font-variant:small-caps;">Dietrich,
    Richard</span> ; <span style="font-variant:small-caps;">Märtlbauer, Erwin</span>
    ; <span style="font-variant:small-caps;">Gareis, Manfred</span>: Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS. In: <i>Food Microbiology</i>
    Bd. 82. London, Academic Press  (2019), S. 75–81'
  havard: S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, M. Gareis, Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS, Food Microbiology. 82
    (2019) 75–81.
  ieee: 'S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, and M. Gareis, “Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS,” <i>Food Microbiology</i>,
    vol. 82, pp. 75–81, 2019, doi: <a href="https://doi.org/10.1016/j.fm.2019.01.012">10.1016/j.fm.2019.01.012</a>.'
  mla: Ulrich, Sebastian, et al. “Identification of Cereulide Producing Bacillus Cereus
    by MALDI-TOF MS.” <i>Food Microbiology</i>, vol. 82, 2019, pp. 75–81, <a href="https://doi.org/10.1016/j.fm.2019.01.012">https://doi.org/10.1016/j.fm.2019.01.012</a>.
  short: S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, M. Gareis, Food Microbiology
    82 (2019) 75–81.
  ufg: '<b>Ulrich, Sebastian u. a.</b>: Identification of cereulide producing Bacillus
    cereus by MALDI-TOF MS, in: <i>Food Microbiology</i> 82 (2019),  S. 75–81.'
  van: Ulrich S, Gottschalk C, Dietrich R, Märtlbauer E, Gareis M. Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS. Food Microbiology. 2019;82:75–81.
date_created: 2025-06-15T10:18:20Z
date_updated: 2025-06-18T11:16:35Z
department:
- _id: DEP4010
doi: 10.1016/j.fm.2019.01.012
extern: '1'
intvolume: '        82'
keyword:
- MALDI-TOF MS
- Bacillus cereus
- Cereulide
- Food intoxication
language:
- iso: eng
page: 75-81
place: London
publication: Food Microbiology
publication_identifier:
  eissn:
  - 1095-9998
  issn:
  - 0740-0020
publication_status: published
publisher: 'Academic Press '
quality_controlled: '1'
status: public
title: Identification of cereulide producing Bacillus cereus by MALDI-TOF MS
type: scientific_journal_article
user_id: '83781'
volume: 82
year: '2019'
...