---
_id: '12958'
abstract:
- lang: eng
  text: Cytotoxic macrocyclic trichothecenes such as satratoxins are produced by chemotype
    S strains of Stachybotrys chartarum. Diseases such as stachybotryotoxicosis in
    animals and the sick building syndrome as a multifactorial disease complex in
    humans have been associated with this mold and its toxins. Less toxic non-chemotype
    S strains of S. chartarum are morphologically indistinguishable from chemotype
    S strains, which results in uncertainties in hazard characterization of isolates.
    To selectively identify macrocyclic trichothecene producing S. chartarum isolates,
    a set of sat14 gene-specific primers was designed and applied in a loop-mediated
    isothermal amplification (LAMP) assay using neutral red for visual signal detection.
    The assay was highly specific for S. chartarum strains of the macrocyclic trichothecene
    producing chemotype and showed no cross-reaction with non-macrocyclic trichothecene
    producing S. chartarum strains or 152 strains of 131 other fungal species. The
    assay’s detection limit was 0.635 pg/rxn (picogram per reaction) with a reaction
    time of 60 min. Its high specificity and sensitivity as well as the cost-saving
    properties make the new assay an interesting and powerful diagnostic tool for
    easy and rapid testing.
author:
- first_name: Johannes
  full_name: Köck, Johannes
  last_name: Köck
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
- first_name: Ludwig
  full_name: Niessen, Ludwig
  last_name: Niessen
citation:
  ama: 'Köck J, Gottschalk C, Ulrich S, Schwaiger K, Gareis M, Niessen L. Rapid and
    selective detection of macrocyclic trichothecene producing Stachybotrys chartarum
    strains by loop-mediated isothermal amplification (LAMP). <i>Analytical and bioanalytical
    chemistry : a merger of Fresenius’ journal of analytical chemistry and Analusis</i>.
    2021;413(19):4801-4813. doi:<a href="https://doi.org/10.1007/s00216-021-03436-y">10.1007/s00216-021-03436-y</a>'
  apa: 'Köck, J., Gottschalk, C., Ulrich, S., Schwaiger, K., Gareis, M., &#38; Niessen,
    L. (2021). Rapid and selective detection of macrocyclic trichothecene producing
    Stachybotrys chartarum strains by loop-mediated isothermal amplification (LAMP).
    <i>Analytical and Bioanalytical Chemistry : A Merger of Fresenius’ Journal of
    Analytical Chemistry and Analusis</i>, <i>413</i>(19), 4801–4813. <a href="https://doi.org/10.1007/s00216-021-03436-y">https://doi.org/10.1007/s00216-021-03436-y</a>'
  bjps: '<b>Köck J <i>et al.</i></b> (2021) Rapid and Selective Detection of Macrocyclic
    Trichothecene Producing Stachybotrys Chartarum Strains by Loop-Mediated Isothermal
    Amplification (LAMP). <i>Analytical and bioanalytical chemistry : a merger of
    Fresenius’ journal of analytical chemistry and Analusis</i> <b>413</b>, 4801–4813.'
  chicago: 'Köck, Johannes, Christoph Gottschalk, Sebastian Ulrich, Karin Schwaiger,
    Manfred Gareis, and Ludwig Niessen. “Rapid and Selective Detection of Macrocyclic
    Trichothecene Producing Stachybotrys Chartarum Strains by Loop-Mediated Isothermal
    Amplification (LAMP).” <i>Analytical and Bioanalytical Chemistry : A Merger of
    Fresenius’ Journal of Analytical Chemistry and Analusis</i> 413, no. 19 (2021):
    4801–13. <a href="https://doi.org/10.1007/s00216-021-03436-y">https://doi.org/10.1007/s00216-021-03436-y</a>.'
  chicago-de: 'Köck, Johannes, Christoph Gottschalk, Sebastian Ulrich, Karin Schwaiger,
    Manfred Gareis und Ludwig Niessen. 2021. Rapid and selective detection of macrocyclic
    trichothecene producing Stachybotrys chartarum strains by loop-mediated isothermal
    amplification (LAMP). <i>Analytical and bioanalytical chemistry : a merger of
    Fresenius’ journal of analytical chemistry and Analusis</i> 413, Nr. 19: 4801–4813.
    doi:<a href="https://doi.org/10.1007/s00216-021-03436-y">10.1007/s00216-021-03436-y</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Köck, Johannes</span> ; <span
    style="font-variant:small-caps;">Gottschalk, Christoph</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Schwaiger, Karin</span>
    ; <span style="font-variant:small-caps;">Gareis, Manfred</span> ; <span style="font-variant:small-caps;">Niessen,
    Ludwig</span>: Rapid and selective detection of macrocyclic trichothecene producing
    Stachybotrys chartarum strains by loop-mediated isothermal amplification (LAMP).
    In: <i>Analytical and bioanalytical chemistry : a merger of Fresenius’ journal
    of analytical chemistry and Analusis</i> Bd. 413. Berlin ; Heidelberg, Springer  (2021),
    Nr. 19, S. 4801–4813'
  havard: 'J. Köck, C. Gottschalk, S. Ulrich, K. Schwaiger, M. Gareis, L. Niessen,
    Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys
    chartarum strains by loop-mediated isothermal amplification (LAMP), Analytical
    and Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry
    and Analusis. 413 (2021) 4801–4813.'
  ieee: 'J. Köck, C. Gottschalk, S. Ulrich, K. Schwaiger, M. Gareis, and L. Niessen,
    “Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys
    chartarum strains by loop-mediated isothermal amplification (LAMP),” <i>Analytical
    and bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry
    and Analusis</i>, vol. 413, no. 19, pp. 4801–4813, 2021, doi: <a href="https://doi.org/10.1007/s00216-021-03436-y">10.1007/s00216-021-03436-y</a>.'
  mla: 'Köck, Johannes, et al. “Rapid and Selective Detection of Macrocyclic Trichothecene
    Producing Stachybotrys Chartarum Strains by Loop-Mediated Isothermal Amplification
    (LAMP).” <i>Analytical and Bioanalytical Chemistry : A Merger of Fresenius’ Journal
    of Analytical Chemistry and Analusis</i>, vol. 413, no. 19, 2021, pp. 4801–13,
    <a href="https://doi.org/10.1007/s00216-021-03436-y">https://doi.org/10.1007/s00216-021-03436-y</a>.'
  short: 'J. Köck, C. Gottschalk, S. Ulrich, K. Schwaiger, M. Gareis, L. Niessen,
    Analytical and Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical
    Chemistry and Analusis 413 (2021) 4801–4813.'
  ufg: '<b>Köck, Johannes u. a.</b>: Rapid and selective detection of macrocyclic
    trichothecene producing Stachybotrys chartarum strains by loop-mediated isothermal
    amplification (LAMP), in: <i>Analytical and bioanalytical chemistry : a merger
    of Fresenius’ journal of analytical chemistry and Analusis</i> 413 (2021), H.
    19,  S. 4801–4813.'
  van: 'Köck J, Gottschalk C, Ulrich S, Schwaiger K, Gareis M, Niessen L. Rapid and
    selective detection of macrocyclic trichothecene producing Stachybotrys chartarum
    strains by loop-mediated isothermal amplification (LAMP). Analytical and bioanalytical
    chemistry : a merger of Fresenius’ journal of analytical chemistry and Analusis.
    2021;413(19):4801–13.'
date_created: 2025-06-15T10:06:17Z
date_updated: 2025-06-16T14:17:01Z
department:
- _id: DEP4010
doi: 10.1007/s00216-021-03436-y
extern: '1'
intvolume: '       413'
issue: '19'
keyword:
- Aspergillus nidulans
- Basidiomycetes
- Fungi
- Strigolactone
- Liquid chromatography
- Solid-phase microextraction
language:
- iso: eng
page: 4801-4813
place: Berlin ; Heidelberg
publication: 'Analytical and bioanalytical chemistry : a merger of Fresenius'' journal
  of analytical chemistry and Analusis'
publication_identifier:
  eissn:
  - 1618-2650
  issn:
  - 1618-2642
publication_status: published
publisher: 'Springer '
quality_controlled: '1'
status: public
title: Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys
  chartarum strains by loop-mediated isothermal amplification (LAMP)
type: scientific_journal_article
user_id: '83781'
volume: 413
year: '2021'
...