[{"user_id":"83781","citation":{"chicago":"Güneş, Zeynep Saliha, İbrahim Gülseren, Carsten Funke, Hans-Jürgen Danneel, and Tuba Esatbeyoglu. “Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities.” <i>Food Chemistry</i> 528 (2026). <a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">https://doi.org/10.1016/j.foodchem.2026.150976</a>.","ama":"Güneş ZS, Gülseren İ, Funke C, Danneel HJ, Esatbeyoglu T. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. <i>Food Chemistry</i>. 2026;528. doi:<a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">10.1016/j.foodchem.2026.150976</a>","din1505-2-1":"<span style=\"font-variant:small-caps;\">Güneş, Zeynep Saliha</span> ; <span style=\"font-variant:small-caps;\">Gülseren, İbrahim</span> ; <span style=\"font-variant:small-caps;\">Funke, Carsten</span> ; <span style=\"font-variant:small-caps;\">Danneel, Hans-Jürgen</span> ; <span style=\"font-variant:small-caps;\">Esatbeyoglu, Tuba</span>: Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. In: <i>Food Chemistry</i> Bd. 528. New York, NY [u.a.], Elsevier BV (2026)","bjps":"<b>Güneş ZS <i>et al.</i></b> (2026) Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities. <i>Food Chemistry</i> <b>528</b>.","van":"Güneş ZS, Gülseren İ, Funke C, Danneel HJ, Esatbeyoglu T. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. Food Chemistry. 2026;528.","ieee":"Z. S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, and T. Esatbeyoglu, “Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities,” <i>Food Chemistry</i>, vol. 528, Art. no. 150976, 2026, doi: <a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">10.1016/j.foodchem.2026.150976</a>.","havard":"Z.S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, T. Esatbeyoglu, Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities, Food Chemistry. 528 (2026).","short":"Z.S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, T. Esatbeyoglu, Food Chemistry 528 (2026).","ufg":"<b>Güneş, Zeynep Saliha u. a.</b>: Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities, in: <i>Food Chemistry</i> 528 (2026).","mla":"Güneş, Zeynep Saliha, et al. “Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities.” <i>Food Chemistry</i>, vol. 528, 150976, 2026, <a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">https://doi.org/10.1016/j.foodchem.2026.150976</a>.","apa":"Güneş, Z. S., Gülseren, İ., Funke, C., Danneel, H.-J., &#38; Esatbeyoglu, T. (2026). Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. <i>Food Chemistry</i>, <i>528</i>, Article 150976. <a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">https://doi.org/10.1016/j.foodchem.2026.150976</a>","chicago-de":"Güneş, Zeynep Saliha, İbrahim Gülseren, Carsten Funke, Hans-Jürgen Danneel und Tuba Esatbeyoglu. 2026. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. <i>Food Chemistry</i> 528. doi:<a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">10.1016/j.foodchem.2026.150976</a>, ."},"place":"New York, NY [u.a.]","volume":528,"publication_status":"published","publication":"Food Chemistry","title":"Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities","publication_identifier":{"issn":["0308-8146"],"eissn":["1873-7072"]},"status":"public","language":[{"iso":"eng"}],"date_created":"2026-09-24T07:39:35Z","type":"scientific_journal_article","article_number":"150976","author":[{"full_name":"Güneş, Zeynep Saliha","last_name":"Güneş","first_name":"Zeynep Saliha"},{"first_name":"İbrahim","last_name":"Gülseren","full_name":"Gülseren, İbrahim"},{"id":"74167","full_name":"Funke, Carsten","first_name":"Carsten","last_name":"Funke"},{"id":"12231","full_name":"Danneel, Hans-Jürgen","last_name":"Danneel","first_name":"Hans-Jürgen"},{"full_name":"Esatbeyoglu, Tuba","last_name":"Esatbeyoglu","first_name":"Tuba"}],"publisher":"Elsevier BV","abstract":[{"text":"Bioactive peptides from plant proteins offer significant benefits. In this study, the structural and bioactive properties of sunflower protein isolate and its enzymatic hydrolysates were systematically investigated. The hydrolysates were fractionated using low-pressure preparative gradient chromatography (LP-PGC), and their antioxidant capacity, along with angiotensin-converting enzyme and dipeptidyl peptidase-IV inhibitory activities relevant to metabolic health, were assessed. Structural analyses via sodium dodecyl sulfate polyacrylamide gel electrophoresis and scanning electron microscopy revealed significant alterations in molecular weight distribution and surface morphology after hydrolysis and fractionation, confirming the generation of low-molecular-weight peptides linked to improved bioactivity. The results demonstrated a clear fraction-dependent bioactivity profile. MTT-based cell viability analysis of HepG2 and Caco-2 cells indicates that the non-toxicity of the hydrolysates depends on both their concentration and the cell type. Molecular docking studies were performed with the three highest-scoring peptides from each hydrolysate according to PeptideRanker. LP-PGC effectively reveals in vitro/in silico bioactivity of sunflower protein hydrolysates for multifunctional food use.","lang":"eng"}],"date_updated":"2026-09-25T09:08:20Z","doi":"10.1016/j.foodchem.2026.150976","intvolume":"       528","department":[{"_id":"DEP4018"}],"quality_controlled":"1","keyword":["Sunflower protein hydrolysates","Fractionation","Bioactive peptides","ACE inhibition","DPP-IV inhibition","Mass spectrometry","In silico analysis"],"year":"2026","_id":"14057"},{"title":"Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence","publication_identifier":{"issn":["0196-9781"]},"publication":"eptides : an international journal ","status":"public","language":[{"iso":"eng"}],"user_id":"83781","place":"Amsterdam [u.a.]","citation":{"ama":"van Walraven N, FitzGerald RJ, Danneel HJ, Amigo-Benavent M. Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence. <i>eptides : an international journal </i>. 2025;193(11). doi:<a href=\"https://doi.org/10.1016/j.peptides.2025.171440\">10.1016/j.peptides.2025.171440</a>","chicago":"Walraven, Nathalie van, Richard J. FitzGerald, Hans-Jürgen Danneel, and Miryam Amigo-Benavent. “Bioactive Peptides in Cosmetic Formulations: Review of Current in Vitro and Ex Vivo Evidence.” <i>Eptides : An International Journal </i> 193, no. 11 (2025). <a href=\"https://doi.org/10.1016/j.peptides.2025.171440\">https://doi.org/10.1016/j.peptides.2025.171440</a>.","mla":"van Walraven, Nathalie, et al. “Bioactive Peptides in Cosmetic Formulations: Review of Current in Vitro and Ex Vivo Evidence.” <i>Eptides : An International Journal </i>, vol. 193, no. 11, 171440, 2025, <a href=\"https://doi.org/10.1016/j.peptides.2025.171440\">https://doi.org/10.1016/j.peptides.2025.171440</a>.","chicago-de":"van Walraven, Nathalie, Richard J. FitzGerald, Hans-Jürgen Danneel und Miryam Amigo-Benavent. 2025. Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence. <i>eptides : an international journal </i> 193, Nr. 11. doi:<a href=\"https://doi.org/10.1016/j.peptides.2025.171440\">10.1016/j.peptides.2025.171440</a>, .","apa":"van Walraven, N., FitzGerald, R. J., Danneel, H.-J., &#38; Amigo-Benavent, M. (2025). Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence. <i>Eptides : An International Journal </i>, <i>193</i>(11), Article 171440. <a href=\"https://doi.org/10.1016/j.peptides.2025.171440\">https://doi.org/10.1016/j.peptides.2025.171440</a>","ufg":"<b>Walraven, Nathalie van u. a.</b>: Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence, in: <i>eptides : an international journal </i> 193 (2025), H. 11.","van":"van Walraven N, FitzGerald RJ, Danneel HJ, Amigo-Benavent M. Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence. eptides : an international journal . 2025;193(11).","ieee":"N. van Walraven, R. J. FitzGerald, H.-J. Danneel, and M. Amigo-Benavent, “Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence,” <i>eptides : an international journal </i>, vol. 193, no. 11, Art. no. 171440, 2025, doi: <a href=\"https://doi.org/10.1016/j.peptides.2025.171440\">10.1016/j.peptides.2025.171440</a>.","short":"N. van Walraven, R.J. FitzGerald, H.-J. Danneel, M. Amigo-Benavent, Eptides : An International Journal  193 (2025).","havard":"N. van Walraven, R.J. FitzGerald, H.-J. Danneel, M. Amigo-Benavent, Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence, Eptides : An International Journal . 193 (2025).","bjps":"<b>van Walraven N <i>et al.</i></b> (2025) Bioactive Peptides in Cosmetic Formulations: Review of Current in Vitro and Ex Vivo Evidence. <i>eptides : an international journal </i> <b>193</b>.","din1505-2-1":"<span style=\"font-variant:small-caps;\">van Walraven, Nathalie</span> ; <span style=\"font-variant:small-caps;\">FitzGerald, Richard J.</span> ; <span style=\"font-variant:small-caps;\">Danneel, Hans-Jürgen</span> ; <span style=\"font-variant:small-caps;\">Amigo-Benavent, Miryam</span>: Bioactive peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence. In: <i>eptides : an international journal </i> Bd. 193. Amsterdam [u.a.], Elsevier BV (2025), Nr. 11"},"volume":193,"publication_status":"published","intvolume":"       193","keyword":["Bioactive peptides","Cosmetic peptides","In vitro studies","Matrikines : Whitening : Antioxidant : Antimicrobial : Neurotransmitter inhibition","Carrier peptides","Immunomodulatory"],"department":[{"_id":"DEP4028"}],"_id":"13221","year":"2025","issue":"11","author":[{"first_name":"Nathalie","last_name":"van Walraven","full_name":"van Walraven, Nathalie","id":"83427"},{"full_name":"FitzGerald, Richard J.","first_name":"Richard J.","last_name":"FitzGerald"},{"id":"12231","full_name":"Danneel, Hans-Jürgen","last_name":"Danneel","first_name":"Hans-Jürgen"},{"first_name":"Miryam","last_name":"Amigo-Benavent","full_name":"Amigo-Benavent, Miryam"}],"type":"scientific_journal_article","date_created":"2025-09-26T15:05:54Z","article_number":"171440","abstract":[{"text":"Bioactive peptides are increasingly employed in cosmetic products and these are generically known as cosmetic peptides. This review aims to provide an update on current information related to commercially available cosmetic peptides, and the in vitro and ex vivo evidence for their potential biological effects. A total of 102 commercially available cosmetic peptides were identified. The majority of these peptides are inspired by molecules already found in the human body, including sequences from extracellular matrix molecules, also known as matrikines. Cosmetic peptides are produced either through chemical synthesis or via biotechnological processes. Their claimed biological activities include signaling to increase collagen and hyaluronic acid production, modulation of pigmentation, maintenance of a healthy skin microbiome, antioxidant activity and cellular defense, immunomodulation, neurotransmitter inhibition, enzyme activity inhibition and trace mineral carriers. The primary structure and current scientific evidence for the bioactivities of these peptides are presented and discussed. The review highlights the diverse methodological approaches used and the outcomes measured in the assessment of cosmetic peptide efficacy. Overall, a large range of cosmetic peptides are commercially available whose efficacy is supported by divergent levels of in vitro and ex vivo data.","lang":"eng"}],"publisher":"Elsevier BV","doi":"10.1016/j.peptides.2025.171440","date_updated":"2025-10-06T13:19:57Z"}]