---
_id: '14057'
abstract:
- lang: eng
  text: Bioactive peptides from plant proteins offer significant benefits. In this
    study, the structural and bioactive properties of sunflower protein isolate and
    its enzymatic hydrolysates were systematically investigated. The hydrolysates
    were fractionated using low-pressure preparative gradient chromatography (LP-PGC),
    and their antioxidant capacity, along with angiotensin-converting enzyme and dipeptidyl
    peptidase-IV inhibitory activities relevant to metabolic health, were assessed.
    Structural analyses via sodium dodecyl sulfate polyacrylamide gel electrophoresis
    and scanning electron microscopy revealed significant alterations in molecular
    weight distribution and surface morphology after hydrolysis and fractionation,
    confirming the generation of low-molecular-weight peptides linked to improved
    bioactivity. The results demonstrated a clear fraction-dependent bioactivity profile.
    MTT-based cell viability analysis of HepG2 and Caco-2 cells indicates that the
    non-toxicity of the hydrolysates depends on both their concentration and the cell
    type. Molecular docking studies were performed with the three highest-scoring
    peptides from each hydrolysate according to PeptideRanker. LP-PGC effectively
    reveals in vitro/in silico bioactivity of sunflower protein hydrolysates for multifunctional
    food use.
article_number: '150976'
author:
- first_name: Zeynep Saliha
  full_name: Güneş, Zeynep Saliha
  last_name: Güneş
- first_name: İbrahim
  full_name: Gülseren, İbrahim
  last_name: Gülseren
- first_name: Carsten
  full_name: Funke, Carsten
  id: '74167'
  last_name: Funke
- first_name: Hans-Jürgen
  full_name: Danneel, Hans-Jürgen
  id: '12231'
  last_name: Danneel
- first_name: Tuba
  full_name: Esatbeyoglu, Tuba
  last_name: Esatbeyoglu
citation:
  ama: 'Güneş ZS, Gülseren İ, Funke C, Danneel HJ, Esatbeyoglu T. Fractionation of
    sunflower protein hydrolysates by low pressure preparative gradient chromatography:
    An integrated in vitro and in silico assessment of bioactivities. <i>Food Chemistry</i>.
    2026;528. doi:<a href="https://doi.org/10.1016/j.foodchem.2026.150976">10.1016/j.foodchem.2026.150976</a>'
  apa: 'Güneş, Z. S., Gülseren, İ., Funke, C., Danneel, H.-J., &#38; Esatbeyoglu,
    T. (2026). Fractionation of sunflower protein hydrolysates by low pressure preparative
    gradient chromatography: An integrated in vitro and in silico assessment of bioactivities.
    <i>Food Chemistry</i>, <i>528</i>, Article 150976. <a href="https://doi.org/10.1016/j.foodchem.2026.150976">https://doi.org/10.1016/j.foodchem.2026.150976</a>'
  bjps: '<b>Güneş ZS <i>et al.</i></b> (2026) Fractionation of Sunflower Protein Hydrolysates
    by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and
    in Silico Assessment of Bioactivities. <i>Food Chemistry</i> <b>528</b>.'
  chicago: 'Güneş, Zeynep Saliha, İbrahim Gülseren, Carsten Funke, Hans-Jürgen Danneel,
    and Tuba Esatbeyoglu. “Fractionation of Sunflower Protein Hydrolysates by Low
    Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico
    Assessment of Bioactivities.” <i>Food Chemistry</i> 528 (2026). <a href="https://doi.org/10.1016/j.foodchem.2026.150976">https://doi.org/10.1016/j.foodchem.2026.150976</a>.'
  chicago-de: 'Güneş, Zeynep Saliha, İbrahim Gülseren, Carsten Funke, Hans-Jürgen
    Danneel und Tuba Esatbeyoglu. 2026. Fractionation of sunflower protein hydrolysates
    by low pressure preparative gradient chromatography: An integrated in vitro and
    in silico assessment of bioactivities. <i>Food Chemistry</i> 528. doi:<a href="https://doi.org/10.1016/j.foodchem.2026.150976">10.1016/j.foodchem.2026.150976</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Güneş, Zeynep Saliha</span>
    ; <span style="font-variant:small-caps;">Gülseren, İbrahim</span> ; <span style="font-variant:small-caps;">Funke,
    Carsten</span> ; <span style="font-variant:small-caps;">Danneel, Hans-Jürgen</span>
    ; <span style="font-variant:small-caps;">Esatbeyoglu, Tuba</span>: Fractionation
    of sunflower protein hydrolysates by low pressure preparative gradient chromatography:
    An integrated in vitro and in silico assessment of bioactivities. In: <i>Food
    Chemistry</i> Bd. 528. New York, NY [u.a.], Elsevier BV (2026)'
  havard: 'Z.S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, T. Esatbeyoglu, Fractionation
    of sunflower protein hydrolysates by low pressure preparative gradient chromatography:
    An integrated in vitro and in silico assessment of bioactivities, Food Chemistry.
    528 (2026).'
  ieee: 'Z. S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, and T. Esatbeyoglu, “Fractionation
    of sunflower protein hydrolysates by low pressure preparative gradient chromatography:
    An integrated in vitro and in silico assessment of bioactivities,” <i>Food Chemistry</i>,
    vol. 528, Art. no. 150976, 2026, doi: <a href="https://doi.org/10.1016/j.foodchem.2026.150976">10.1016/j.foodchem.2026.150976</a>.'
  mla: 'Güneş, Zeynep Saliha, et al. “Fractionation of Sunflower Protein Hydrolysates
    by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and
    in Silico Assessment of Bioactivities.” <i>Food Chemistry</i>, vol. 528, 150976,
    2026, <a href="https://doi.org/10.1016/j.foodchem.2026.150976">https://doi.org/10.1016/j.foodchem.2026.150976</a>.'
  short: Z.S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, T. Esatbeyoglu, Food Chemistry
    528 (2026).
  ufg: '<b>Güneş, Zeynep Saliha u. a.</b>: Fractionation of sunflower protein hydrolysates
    by low pressure preparative gradient chromatography: An integrated in vitro and
    in silico assessment of bioactivities, in: <i>Food Chemistry</i> 528 (2026).'
  van: 'Güneş ZS, Gülseren İ, Funke C, Danneel HJ, Esatbeyoglu T. Fractionation of
    sunflower protein hydrolysates by low pressure preparative gradient chromatography:
    An integrated in vitro and in silico assessment of bioactivities. Food Chemistry.
    2026;528.'
date_created: 2026-09-24T07:39:35Z
date_updated: 2026-09-25T09:08:20Z
department:
- _id: DEP4018
doi: 10.1016/j.foodchem.2026.150976
intvolume: '       528'
keyword:
- Sunflower protein hydrolysates
- Fractionation
- Bioactive peptides
- ACE inhibition
- DPP-IV inhibition
- Mass spectrometry
- In silico analysis
language:
- iso: eng
place: New York, NY [u.a.]
publication: Food Chemistry
publication_identifier:
  eissn:
  - 1873-7072
  issn:
  - 0308-8146
publication_status: published
publisher: Elsevier BV
quality_controlled: '1'
status: public
title: 'Fractionation of sunflower protein hydrolysates by low pressure preparative
  gradient chromatography: An integrated in vitro and in silico assessment of bioactivities'
type: scientific_journal_article
user_id: '83781'
volume: 528
year: '2026'
...
---
_id: '13221'
abstract:
- lang: eng
  text: Bioactive peptides are increasingly employed in cosmetic products and these
    are generically known as cosmetic peptides. This review aims to provide an update
    on current information related to commercially available cosmetic peptides, and
    the in vitro and ex vivo evidence for their potential biological effects. A total
    of 102 commercially available cosmetic peptides were identified. The majority
    of these peptides are inspired by molecules already found in the human body, including
    sequences from extracellular matrix molecules, also known as matrikines. Cosmetic
    peptides are produced either through chemical synthesis or via biotechnological
    processes. Their claimed biological activities include signaling to increase collagen
    and hyaluronic acid production, modulation of pigmentation, maintenance of a healthy
    skin microbiome, antioxidant activity and cellular defense, immunomodulation,
    neurotransmitter inhibition, enzyme activity inhibition and trace mineral carriers.
    The primary structure and current scientific evidence for the bioactivities of
    these peptides are presented and discussed. The review highlights the diverse
    methodological approaches used and the outcomes measured in the assessment of
    cosmetic peptide efficacy. Overall, a large range of cosmetic peptides are commercially
    available whose efficacy is supported by divergent levels of in vitro and ex vivo
    data.
article_number: '171440'
author:
- first_name: Nathalie
  full_name: van Walraven, Nathalie
  id: '83427'
  last_name: van Walraven
- first_name: Richard J.
  full_name: FitzGerald, Richard J.
  last_name: FitzGerald
- first_name: Hans-Jürgen
  full_name: Danneel, Hans-Jürgen
  id: '12231'
  last_name: Danneel
- first_name: Miryam
  full_name: Amigo-Benavent, Miryam
  last_name: Amigo-Benavent
citation:
  ama: 'van Walraven N, FitzGerald RJ, Danneel HJ, Amigo-Benavent M. Bioactive peptides
    in cosmetic formulations: Review of current in vitro and ex vivo evidence. <i>eptides :
    an international journal </i>. 2025;193(11). doi:<a href="https://doi.org/10.1016/j.peptides.2025.171440">10.1016/j.peptides.2025.171440</a>'
  apa: 'van Walraven, N., FitzGerald, R. J., Danneel, H.-J., &#38; Amigo-Benavent,
    M. (2025). Bioactive peptides in cosmetic formulations: Review of current in vitro
    and ex vivo evidence. <i>Eptides : An International Journal </i>, <i>193</i>(11),
    Article 171440. <a href="https://doi.org/10.1016/j.peptides.2025.171440">https://doi.org/10.1016/j.peptides.2025.171440</a>'
  bjps: '<b>van Walraven N <i>et al.</i></b> (2025) Bioactive Peptides in Cosmetic
    Formulations: Review of Current in Vitro and Ex Vivo Evidence. <i>eptides : an
    international journal </i> <b>193</b>.'
  chicago: 'Walraven, Nathalie van, Richard J. FitzGerald, Hans-Jürgen Danneel, and
    Miryam Amigo-Benavent. “Bioactive Peptides in Cosmetic Formulations: Review of
    Current in Vitro and Ex Vivo Evidence.” <i>Eptides : An International Journal
    </i> 193, no. 11 (2025). <a href="https://doi.org/10.1016/j.peptides.2025.171440">https://doi.org/10.1016/j.peptides.2025.171440</a>.'
  chicago-de: 'van Walraven, Nathalie, Richard J. FitzGerald, Hans-Jürgen Danneel
    und Miryam Amigo-Benavent. 2025. Bioactive peptides in cosmetic formulations:
    Review of current in vitro and ex vivo evidence. <i>eptides : an international
    journal </i> 193, Nr. 11. doi:<a href="https://doi.org/10.1016/j.peptides.2025.171440">10.1016/j.peptides.2025.171440</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">van Walraven, Nathalie</span>
    ; <span style="font-variant:small-caps;">FitzGerald, Richard J.</span> ; <span
    style="font-variant:small-caps;">Danneel, Hans-Jürgen</span> ; <span style="font-variant:small-caps;">Amigo-Benavent,
    Miryam</span>: Bioactive peptides in cosmetic formulations: Review of current
    in vitro and ex vivo evidence. In: <i>eptides : an international journal </i>
    Bd. 193. Amsterdam [u.a.], Elsevier BV (2025), Nr. 11'
  havard: 'N. van Walraven, R.J. FitzGerald, H.-J. Danneel, M. Amigo-Benavent, Bioactive
    peptides in cosmetic formulations: Review of current in vitro and ex vivo evidence,
    Eptides : An International Journal . 193 (2025).'
  ieee: 'N. van Walraven, R. J. FitzGerald, H.-J. Danneel, and M. Amigo-Benavent,
    “Bioactive peptides in cosmetic formulations: Review of current in vitro and ex
    vivo evidence,” <i>eptides : an international journal </i>, vol. 193, no. 11,
    Art. no. 171440, 2025, doi: <a href="https://doi.org/10.1016/j.peptides.2025.171440">10.1016/j.peptides.2025.171440</a>.'
  mla: 'van Walraven, Nathalie, et al. “Bioactive Peptides in Cosmetic Formulations:
    Review of Current in Vitro and Ex Vivo Evidence.” <i>Eptides : An International
    Journal </i>, vol. 193, no. 11, 171440, 2025, <a href="https://doi.org/10.1016/j.peptides.2025.171440">https://doi.org/10.1016/j.peptides.2025.171440</a>.'
  short: 'N. van Walraven, R.J. FitzGerald, H.-J. Danneel, M. Amigo-Benavent, Eptides :
    An International Journal  193 (2025).'
  ufg: '<b>Walraven, Nathalie van u. a.</b>: Bioactive peptides in cosmetic formulations:
    Review of current in vitro and ex vivo evidence, in: <i>eptides : an international
    journal </i> 193 (2025), H. 11.'
  van: 'van Walraven N, FitzGerald RJ, Danneel HJ, Amigo-Benavent M. Bioactive peptides
    in cosmetic formulations: Review of current in vitro and ex vivo evidence. eptides :
    an international journal . 2025;193(11).'
date_created: 2025-09-26T15:05:54Z
date_updated: 2025-10-06T13:19:57Z
department:
- _id: DEP4028
doi: 10.1016/j.peptides.2025.171440
intvolume: '       193'
issue: '11'
keyword:
- Bioactive peptides
- Cosmetic peptides
- In vitro studies
- 'Matrikines : Whitening : Antioxidant : Antimicrobial : Neurotransmitter inhibition'
- Carrier peptides
- Immunomodulatory
language:
- iso: eng
place: Amsterdam [u.a.]
publication: 'eptides : an international journal '
publication_identifier:
  issn:
  - 0196-9781
publication_status: published
publisher: Elsevier BV
status: public
title: 'Bioactive peptides in cosmetic formulations: Review of current in vitro and
  ex vivo evidence'
type: scientific_journal_article
user_id: '83781'
volume: 193
year: '2025'
...