---
_id: '12942'
abstract:
- lang: eng
  text: The ectoparasite Ixodes ricinus is an important vector for many tick-borne
    diseases (TBD) in the northern hemisphere, such as Lyme borreliosis, rickettsiosis,
    human granulocytic anaplasmosis, or tick-borne encephalitis virus. As climate
    change will lead to rising temperatures in the next years, we expect an increase
    in tick activity, tick population, and thus in the spread of TBD. Consequently,
    it has never been more critical to understand relationships within the microbial
    communities in ticks that might contribute to the tick’s fitness and the occurrence
    of TBD. Therefore, we analyzed the microbiota in different tick tissues such as
    midgut, salivary glands, and residual tick material, as well as the microbiota
    in complete Ixodes ricinus ticks using 16S rRNA gene amplicon sequencing. By using
    a newly developed DNA extraction protocol for tick tissue samples and a self-designed
    mock community, we were able to detect endosymbionts and pathogens that have been
    described in the literature previously. Further, this study displayed the usefulness
    of including a mock community during bioinformatic analysis to identify essential
    bacteria within the tick.
article_number: '1100'
author:
- first_name: Anna
  full_name: Wiesinger, Anna
  last_name: Wiesinger
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Stephanie
  full_name: Hiereth, Stephanie
  last_name: Hiereth
- first_name: Lidia
  full_name: Chitimia-Dobler, Lidia
  last_name: Chitimia-Dobler
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: 'Wiesinger A, Wenderlein J, Ulrich S, Hiereth S, Chitimia-Dobler L, Straubinger
    RK. Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota
    of Female Ixodes ricinus Ticks. <i>International Journal of Molecular Sciences</i>.
    2023;24(2). doi:<a href="https://doi.org/10.3390/ijms24021100">10.3390/ijms24021100</a>'
  apa: 'Wiesinger, A., Wenderlein, J., Ulrich, S., Hiereth, S., Chitimia-Dobler, L.,
    &#38; Straubinger, R. K. (2023). Revealing the Tick Microbiome: Insights into
    Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks. <i>International
    Journal of Molecular Sciences</i>, <i>24</i>(2), Article 1100. <a href="https://doi.org/10.3390/ijms24021100">https://doi.org/10.3390/ijms24021100</a>'
  bjps: '<b>Wiesinger A <i>et al.</i></b> (2023) Revealing the Tick Microbiome: Insights
    into Midgut and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks. <i>International
    Journal of Molecular Sciences</i> <b>24</b>.'
  chicago: 'Wiesinger, Anna, Jasmin Wenderlein, Sebastian Ulrich, Stephanie Hiereth,
    Lidia Chitimia-Dobler, and Reinhard K. Straubinger. “Revealing the Tick Microbiome:
    Insights into Midgut and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks.”
    <i>International Journal of Molecular Sciences</i> 24, no. 2 (2023). <a href="https://doi.org/10.3390/ijms24021100">https://doi.org/10.3390/ijms24021100</a>.'
  chicago-de: 'Wiesinger, Anna, Jasmin Wenderlein, Sebastian Ulrich, Stephanie Hiereth,
    Lidia Chitimia-Dobler und Reinhard K. Straubinger. 2023. Revealing the Tick Microbiome:
    Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks.
    <i>International Journal of Molecular Sciences</i> 24, Nr. 2. doi:<a href="https://doi.org/10.3390/ijms24021100">10.3390/ijms24021100</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Wiesinger, Anna</span> ; <span
    style="font-variant:small-caps;">Wenderlein, Jasmin</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Hiereth, Stephanie</span>
    ; <span style="font-variant:small-caps;">Chitimia-Dobler, Lidia</span> ; <span
    style="font-variant:small-caps;">Straubinger, Reinhard K.</span>: Revealing the
    Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female
    Ixodes ricinus Ticks. In: <i>International Journal of Molecular Sciences</i> Bd.
    24. Basel, MDPI AG (2023), Nr. 2'
  havard: 'A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler,
    R.K. Straubinger, Revealing the Tick Microbiome: Insights into Midgut and Salivary
    Gland Microbiota of Female Ixodes ricinus Ticks, International Journal of Molecular
    Sciences. 24 (2023).'
  ieee: 'A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler, and
    R. K. Straubinger, “Revealing the Tick Microbiome: Insights into Midgut and Salivary
    Gland Microbiota of Female Ixodes ricinus Ticks,” <i>International Journal of
    Molecular Sciences</i>, vol. 24, no. 2, Art. no. 1100, 2023, doi: <a href="https://doi.org/10.3390/ijms24021100">10.3390/ijms24021100</a>.'
  mla: 'Wiesinger, Anna, et al. “Revealing the Tick Microbiome: Insights into Midgut
    and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks.” <i>International
    Journal of Molecular Sciences</i>, vol. 24, no. 2, 1100, 2023, <a href="https://doi.org/10.3390/ijms24021100">https://doi.org/10.3390/ijms24021100</a>.'
  short: A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler, R.K.
    Straubinger, International Journal of Molecular Sciences 24 (2023).
  ufg: '<b>Wiesinger, Anna u. a.</b>: Revealing the Tick Microbiome: Insights into
    Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks, in: <i>International
    Journal of Molecular Sciences</i> 24 (2023), H. 2.'
  van: 'Wiesinger A, Wenderlein J, Ulrich S, Hiereth S, Chitimia-Dobler L, Straubinger
    RK. Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota
    of Female Ixodes ricinus Ticks. International Journal of Molecular Sciences. 2023;24(2).'
date_created: 2025-06-15T09:53:01Z
date_updated: 2025-06-16T09:25:29Z
department:
- _id: DEP4010
doi: 10.3390/ijms24021100
extern: '1'
intvolume: '        24'
issue: '2'
keyword:
- Ixodes ricinus
- microbiome
- tick-borne disease
- salivary glands
- midgut
- endosymbiont
- Candidatus Midichloria mitochondrii
language:
- iso: eng
place: Basel
publication: International Journal of Molecular Sciences
publication_identifier:
  eissn:
  - 1422-0067
  issn:
  - 1661-6596
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: 'Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota
  of Female Ixodes ricinus Ticks'
type: scientific_journal_article
user_id: '83781'
volume: 24
year: '2023'
...