---
_id: '13315'
abstract:
- lang: eng
  text: Determining cell density and viability with (semi‐)automated methods enables
    rapid cell cultivation analysis. However, scientific validation is required, typically
    by comparing results to manual counting. To address this, we present a method
    to evaluate and compare two cell counting methods exemplified by a manual and
    a semi‐automated method (Countstar BioTech). Experiments followed validation parameters
    aligned with the International Council for Harmonization (ICH) Q2(R1) guideline
    and a dilution series design based on ISO 20391‐2:2019. Both the semi‐automated
    and manual methods showed comparable specificity and linearity for Chinese hamster
    ovary (CHO)‐K1 cells. The semi‐automated method exhibited superior repeatability
    for total cell density, whereas cell viability results showed no significant difference.
author:
- first_name: Selina
  full_name: Uhlendorff, Selina
  id: '68713'
  last_name: Uhlendorff
  orcid: https://orcid.org/0000-0002-0502-8032
- first_name: Ulrich
  full_name: Odefey, Ulrich
  id: '74218'
  last_name: Odefey
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
- first_name: Miriam
  full_name: Pein-Hackelbusch, Miriam
  id: '64952'
  last_name: Pein-Hackelbusch
  orcid: 0000-0002-7920-0595
citation:
  ama: Uhlendorff S, Odefey U, Frahm B, Pein-Hackelbusch M. Performance Comparison
    between Semi‐Automated and Manual Cell Counting for Animal Cell Culture. <i>Chemie
    Ingenieur Technik</i>. 2025;98(1-2):57-68. doi:<a href="https://doi.org/10.1002/cite.70048">10.1002/cite.70048</a>
  apa: Uhlendorff, S., Odefey, U., Frahm, B., &#38; Pein-Hackelbusch, M. (2025). Performance
    Comparison between Semi‐Automated and Manual Cell Counting for Animal Cell Culture.
    <i>Chemie Ingenieur Technik</i>, <i>98</i>(1–2), 57–68. <a href="https://doi.org/10.1002/cite.70048">https://doi.org/10.1002/cite.70048</a>
  bjps: <b>Uhlendorff S <i>et al.</i></b> (2025) Performance Comparison between Semi‐Automated
    and Manual Cell Counting for Animal Cell Culture. <i>Chemie Ingenieur Technik</i>
    <b>98</b>, 57–68.
  chicago: 'Uhlendorff, Selina, Ulrich Odefey, Björn Frahm, and Miriam Pein-Hackelbusch.
    “Performance Comparison between Semi‐Automated and Manual Cell Counting for Animal
    Cell Culture.” <i>Chemie Ingenieur Technik</i> 98, no. 1–2 (2025): 57–68. <a href="https://doi.org/10.1002/cite.70048">https://doi.org/10.1002/cite.70048</a>.'
  chicago-de: 'Uhlendorff, Selina, Ulrich Odefey, Björn Frahm und Miriam Pein-Hackelbusch.
    2025. Performance Comparison between Semi‐Automated and Manual Cell Counting for
    Animal Cell Culture. <i>Chemie Ingenieur Technik</i> 98, Nr. 1–2: 57–68. doi:<a
    href="https://doi.org/10.1002/cite.70048">10.1002/cite.70048</a>, .'
  din1505-2-1: '<span style="font-variant:small-caps;">Uhlendorff, Selina</span> ;
    <span style="font-variant:small-caps;">Odefey, Ulrich</span> ; <span style="font-variant:small-caps;">Frahm,
    Björn</span> ; <span style="font-variant:small-caps;">Pein-Hackelbusch, Miriam</span>:
    Performance Comparison between Semi‐Automated and Manual Cell Counting for Animal
    Cell Culture. In: <i>Chemie Ingenieur Technik</i> Bd. 98. Weinheim, Wiley (2025),
    Nr. 1–2, S. 57–68'
  havard: S. Uhlendorff, U. Odefey, B. Frahm, M. Pein-Hackelbusch, Performance Comparison
    between Semi‐Automated and Manual Cell Counting for Animal Cell Culture, Chemie
    Ingenieur Technik. 98 (2025) 57–68.
  ieee: 'S. Uhlendorff, U. Odefey, B. Frahm, and M. Pein-Hackelbusch, “Performance
    Comparison between Semi‐Automated and Manual Cell Counting for Animal Cell Culture,”
    <i>Chemie Ingenieur Technik</i>, vol. 98, no. 1–2, pp. 57–68, 2025, doi: <a href="https://doi.org/10.1002/cite.70048">10.1002/cite.70048</a>.'
  mla: Uhlendorff, Selina, et al. “Performance Comparison between Semi‐Automated and
    Manual Cell Counting for Animal Cell Culture.” <i>Chemie Ingenieur Technik</i>,
    vol. 98, no. 1–2, 2025, pp. 57–68, <a href="https://doi.org/10.1002/cite.70048">https://doi.org/10.1002/cite.70048</a>.
  short: S. Uhlendorff, U. Odefey, B. Frahm, M. Pein-Hackelbusch, Chemie Ingenieur
    Technik 98 (2025) 57–68.
  ufg: '<b>Uhlendorff, Selina u. a.</b>: Performance Comparison between Semi‐Automated
    and Manual Cell Counting for Animal Cell Culture, in: <i>Chemie Ingenieur Technik</i>
    98 (2025), H. 1–2,  S. 57–68.'
  van: Uhlendorff S, Odefey U, Frahm B, Pein-Hackelbusch M. Performance Comparison
    between Semi‐Automated and Manual Cell Counting for Animal Cell Culture. Chemie
    Ingenieur Technik. 2025;98(1–2):57–68.
date_created: 2025-12-08T07:26:52Z
date_updated: 2026-03-27T17:14:42Z
department:
- _id: DEP4029
- _id: DEP4028
doi: 10.1002/cite.70048
intvolume: '        98'
issue: 1-2
keyword:
- Cell density
- Cell enumeration
- Cell viability
- CHO-K1
language:
- iso: eng
main_file_link:
- open_access: '1'
oa: '1'
page: 57–68
place: Weinheim
publication: Chemie Ingenieur Technik
publication_identifier:
  eissn:
  - 1522-2640
  issn:
  - 0009-286X
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: Performance Comparison between Semi‐Automated and Manual Cell Counting for
  Animal Cell Culture
type: scientific_journal_article
user_id: '45666'
volume: 98
year: '2025'
...