---
_id: '12983'
abstract:
- lang: eng
text: "Background\r\nA broad spectrum of pathogens is causative for respiratory
tract infections, but symptoms are mostly similar. Therefore, the identification
of the causative viruses and bacteria is only feasible using multiplex PCR or
several monoplex PCR tests in parallel.\r\nMethods\r\nThe analytical sensitivity
of three multiplex PCR assays, RespiFinder-19, RespiFinder-SMART-22 and xTAG-Respiratory-Virus-Panel-Fast-Assay
(RVP), were compared to monoplex real-time PCR with quantified standardized control
material. All assays include the most common respiratory pathogens.\r\nResults\r\nTo
compare the analytical sensitivity of the multiplex assays, samples were inoculated
with 13 different quantified viruses in the range of 101 to 105 copies/ml. Concordant
results were received for rhinovirus, whereas the RVP detected influenzavirus,
RSV and hMPV more frequently in low concentrations. The RespiFinder-19 and the
RespiFinder-SMART-22 showed a higher analytical sensitivity for adenoviruses and
coronaviruses, whereas the RVP was incapable to detect adenovirus and coronavirus
in concentrations of 104 copies/ml. The RespiFinder-19 and RespiFinder-SMART-22A
did not detect influenzaviruses (104 copies/ml) and RSV (103 copies/ml). The detection
of all 13 viruses in one sample was only achieved using monoplex PCR. To analyze
possible competitive amplification reactions between the different viruses, samples
were further inoculated with only 4 different viruses in one sample. Compared
to the detection of 13 viruses in parallel, only a few differences were found.\r\nThe
incidence of respiratory viruses was compared in tracheal secretion (TS) samples
(n = 100) of mechanically ventilated patients in winter (n = 50) and summer (n = 50).
In winter, respiratory viruses were detected in 32 TS samples (64%) by RespiFinder-19,
whereas the detection rate with RVP was only 22%. The most frequent viruses were
adenovirus (32%) and PIV-2 (20%). Multiple infections were detected in 16 TS samples
(32%) by RespiFinder-19. Fewer infections were found in summer (RespiFinder-19:
20%; RVP: 6%). All positive results were verified using monoplex PCR.\r\nConclusions\r\nMultiplex
PCR tests have a broad spectrum of pathogens to test at a time. Analysis of multiple
inoculated samples revealed a different focus of the detected virus types by the
three assays. Analysis of clinical samples showed a high concordance of detected
viruses by the RespiFinder-19 compared to monoplex tests."
article_number: '163'
author:
- first_name: Mareike
full_name: Dabisch-Ruthe, Mareike
id: '66516'
last_name: Dabisch-Ruthe
orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Tanja
full_name: Vollmer, Tanja
last_name: Vollmer
- first_name: Ortwin
full_name: Adams, Ortwin
last_name: Adams
- first_name: Cornelius
full_name: Knabbe, Cornelius
last_name: Knabbe
- first_name: Jens
full_name: Dreier, Jens
last_name: Dreier
citation:
ama: 'Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. BMC Infectious Diseases. 2012;12(1). doi:10.1186/1471-2334-12-163'
apa: 'Dabisch-Ruthe, M., Vollmer, T., Adams, O., Knabbe, C., & Dreier, J. (2012).
Comparison of three multiplex PCR assays for the detection of respiratory viral
infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder
19 assay and RespiFinder SMART 22 assay. BMC Infectious Diseases, 12(1),
Article 163. https://doi.org/10.1186/1471-2334-12-163'
bjps: 'Dabisch-Ruthe M et al. (2012) Comparison of Three Multiplex
PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG
Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART
22 Assay. BMC Infectious Diseases 12.'
chicago: 'Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe,
and Jens Dreier. “Comparison of Three Multiplex PCR Assays for the Detection of
Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast
Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” BMC Infectious
Diseases 12, no. 1 (2012). https://doi.org/10.1186/1471-2334-12-163.'
chicago-de: 'Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe
und Jens Dreier. 2012. Comparison of three multiplex PCR assays for the detection
of respiratory viral infections: evaluation of xTAG respiratory virus panel fast
assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infectious
Diseases 12, Nr. 1. doi:10.1186/1471-2334-12-163,
.'
din1505-2-1: 'Dabisch-Ruthe, Mareike
; Vollmer, Tanja ; Adams,
Ortwin ; Knabbe, Cornelius
; Dreier, Jens: Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. In: BMC Infectious Diseases Bd. 12. Berlin ; Heidelberg,
Springer (2012), Nr. 1'
havard: 'M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, Comparison
of three multiplex PCR assays for the detection of respiratory viral infections:
evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and
RespiFinder SMART 22 assay, BMC Infectious Diseases. 12 (2012).'
ieee: 'M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, and J. Dreier, “Comparison
of three multiplex PCR assays for the detection of respiratory viral infections:
evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and
RespiFinder SMART 22 assay,” BMC Infectious Diseases, vol. 12, no. 1, Art.
no. 163, 2012, doi: 10.1186/1471-2334-12-163.'
mla: 'Dabisch-Ruthe, Mareike, et al. “Comparison of Three Multiplex PCR Assays for
the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory
Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.”
BMC Infectious Diseases, vol. 12, no. 1, 163, 2012, https://doi.org/10.1186/1471-2334-12-163.'
short: M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, BMC Infectious
Diseases 12 (2012).
ufg: 'Dabisch-Ruthe, Mareike u. a.: Comparison of three multiplex PCR assays
for the detection of respiratory viral infections: evaluation of xTAG respiratory
virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, in:
BMC Infectious Diseases 12 (2012), H. 1.'
van: 'Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three
multiplex PCR assays for the detection of respiratory viral infections: evaluation
of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
SMART 22 assay. BMC Infectious Diseases. 2012;12(1).'
date_created: 2025-06-17T06:52:13Z
date_updated: 2025-06-17T13:49:03Z
department:
- _id: DEP4010
doi: 10.1186/1471-2334-12-163
intvolume: ' 12'
issue: '1'
keyword:
- Respiratory Virus
- Elution Volume
- Multiplex Assay
- Multiple Infection
- Bocavirus
language:
- iso: eng
place: Berlin ; Heidelberg
publication: BMC Infectious Diseases
publication_identifier:
eissn:
- 1471-2334
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: 'Comparison of three multiplex PCR assays for the detection of respiratory
viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder
19 assay and RespiFinder SMART 22 assay'
type: scientific_journal_article
user_id: '83781'
volume: 12
year: '2012'
...