---
_id: '12977'
abstract:
- lang: eng
text: 'Stachybotrys (S.) spp. are omnipresent cellulolytic molds. Some species are
highly toxic owing to their ability to synthesize various secondary metabolites
such as macrocyclic trichothecenes or hemolysins. The reliable identification
of Stachybotrys at species level is currently limited to genome-based identification.
This study aimed to establish a fast and reliable MALDI-TOF MS identification
method by optimizing the pre-analytical steps for protein extraction for subsequent
generation of high-quality fingerprint mass spectra. Eight reference strains of
the American Type Culture Collection and the Technical University of Denmark were
cultivated in triplicate (biological repetitions) for 2 days in malt extract broth.
The mycelia (1.5 ml) were first washed with 75 % ethanol and an additional washing
step with dimethyl sulfoxide (10 %) was added to remove unspecific low weight
masses. Furthermore, mycelia were broken with roughened glass beads in formic
acid (70 %) and acetonitrile. The method was successfully applied to a total of
45 isolates of Stachybotrys originating from three different habitats (indoor,
feed, and food samples; n = 15 each): Twenty-seven isolates of S. chartarum and
18 isolates of S. chlorohalonata could be identified by MALDI-TOF MS. The data
obtained exactly matched those obtained by genome-based identification. The mean
score values for S. chartarum ranged from 2.509 to 2.739 and from 2.148 to 2.622
for S. chlorohalonata with a very good reproducibility: the relative standard
deviations were between 0.3 % and 6.8 %. Thus, MALDI-TOF MS proved to be a fast
and reliable alternative to identification of Stachybotrys spp. by nucleotide
amplification and sequencing.'
author:
- first_name: Sebastian
full_name: Ulrich, Sebastian
id: '85847'
last_name: Ulrich
orcid: 0000-0002-4511-9537
- first_name: Barbara
full_name: Biermaier, Barbara
last_name: Biermaier
- first_name: Oliver
full_name: Bader, Oliver
last_name: Bader
- first_name: Georg
full_name: Wolf, Georg
last_name: Wolf
- first_name: Reinhard K.
full_name: Straubinger, Reinhard K.
last_name: Straubinger
- first_name: Andrea
full_name: Didier, Andrea
last_name: Didier
- first_name: Brigitte
full_name: Sperner, Brigitte
last_name: Sperner
- first_name: Karin
full_name: Schwaiger, Karin
last_name: Schwaiger
- first_name: Manfred
full_name: Gareis, Manfred
last_name: Gareis
- first_name: Christoph
full_name: Gottschalk, Christoph
last_name: Gottschalk
citation:
ama: 'Ulrich S, Biermaier B, Bader O, et al. Identification of Stachybotrys spp.
by MALDI-TOF mass spectrometry. Analytical & bioanalytical chemistry :
a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica.
2016;408(27):7565-7581. doi:10.1007/s00216-016-9800-9'
apa: 'Ulrich, S., Biermaier, B., Bader, O., Wolf, G., Straubinger, R. K., Didier,
A., Sperner, B., Schwaiger, K., Gareis, M., & Gottschalk, C. (2016). Identification
of Stachybotrys spp. by MALDI-TOF mass spectrometry. Analytical & Bioanalytical
Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and
Quimica Analitica, 408(27), 7565–7581. https://doi.org/10.1007/s00216-016-9800-9'
bjps: 'Ulrich S et al. (2016) Identification of Stachybotrys Spp.
by MALDI-TOF Mass Spectrometry. Analytical & bioanalytical chemistry :
a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica
408, 7565–7581.'
chicago: 'Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard
K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis,
and Christoph Gottschalk. “Identification of Stachybotrys Spp. by MALDI-TOF Mass
Spectrometry.” Analytical & Bioanalytical Chemistry : A Merger of Fresenius’
Journal of Analytical Chemistry, Analusis and Quimica Analitica 408, no. 27
(2016): 7565–81. https://doi.org/10.1007/s00216-016-9800-9.'
chicago-de: 'Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard
K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis
und Christoph Gottschalk. 2016. Identification of Stachybotrys spp. by MALDI-TOF
mass spectrometry. Analytical & bioanalytical chemistry : a merger of
Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica
408, Nr. 27: 7565–7581. doi:10.1007/s00216-016-9800-9,
.'
din1505-2-1: 'Ulrich,
Sebastian ; Biermaier, Barbara
; Bader, Oliver ; Wolf,
Georg ; Straubinger, Reinhard K.
; Didier, Andrea ; Sperner,
Brigitte ; Schwaiger, Karin
; u. a.: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry.
In: Analytical & bioanalytical chemistry : a merger of Fresenius’ journal
of analytical chemistry, Analusis and Quimica analitica Bd. 408. Berlin ;
Heidelberg, Springer (2016), Nr. 27, S. 7565–7581'
havard: 'S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier,
B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Identification of Stachybotrys
spp. by MALDI-TOF mass spectrometry, Analytical & Bioanalytical Chemistry :
A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica.
408 (2016) 7565–7581.'
ieee: 'S. Ulrich et al., “Identification of Stachybotrys spp. by MALDI-TOF
mass spectrometry,” Analytical & bioanalytical chemistry : a merger of
Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica,
vol. 408, no. 27, pp. 7565–7581, 2016, doi: 10.1007/s00216-016-9800-9.'
mla: 'Ulrich, Sebastian, et al. “Identification of Stachybotrys Spp. by MALDI-TOF
Mass Spectrometry.” Analytical & Bioanalytical Chemistry : A Merger of
Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica,
vol. 408, no. 27, 2016, pp. 7565–81, https://doi.org/10.1007/s00216-016-9800-9.'
short: 'S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier,
B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Analytical & Bioanalytical
Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and
Quimica Analitica 408 (2016) 7565–7581.'
ufg: 'Ulrich, Sebastian u. a.: Identification of Stachybotrys spp. by MALDI-TOF
mass spectrometry, in: Analytical & bioanalytical chemistry : a merger
of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica
408 (2016), H. 27, S. 7565–7581.'
van: 'Ulrich S, Biermaier B, Bader O, Wolf G, Straubinger RK, Didier A, et al. Identification
of Stachybotrys spp. by MALDI-TOF mass spectrometry. Analytical & bioanalytical
chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and
Quimica analitica. 2016;408(27):7565–81.'
date_created: 2025-06-15T10:32:52Z
date_updated: 2025-06-18T12:11:46Z
department:
- _id: DEP4010
doi: 10.1007/s00216-016-9800-9
extern: '1'
intvolume: ' 408'
issue: '27'
keyword:
- Stachybotrys spp
- MALDI-TOF MS
- Mass spectrometry
- Filamentous fungi
language:
- iso: eng
page: 7565-7581
place: Berlin ; Heidelberg
publication: ' Analytical & bioanalytical chemistry : a merger of Fresenius'' journal
of analytical chemistry, Analusis and Quimica analitica'
publication_identifier:
eissn:
- 1618-2650
issn:
- 1618-2642
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry
type: scientific_journal_article
user_id: '83781'
volume: 408
year: '2016'
...