[{"department":[{"_id":"DEP4010"}],"year":"2017","user_id":"83781","status":"public","keyword":["MALDI-TOF","Mass spectrometry","Freshness","Fish","Quality control","Authenticity"],"extern":"1","page":"281-289","abstract":[{"lang":"eng","text":"Properly handled fish is usually marketed as “fresh fish” until day 10 after fishing. About 40% of the total fishery that is used for direct human consumption is marketed in fresh form stored at temperatures up to +2 °C. Currently, there are no validated methods available for controlling the recommended period of storage. Apart from being a potential source for food fraud, spoiled fish represents a major source of foodborne illnesses and intoxications.\r\nIn this study, a rapid MALDI-TOF mass spectrometry based screening method was developed using the vitreous fluid of fish eyes as specimen for the examination of different days of storage. The vitreous fluid was collected from n = 100 freshly fished brown trouts at day 0, 3, 7, 9, and 11 post mortem (n = 20 brown trouts each day of examination). The samples were immediately measured by MALDI-TOF mass spectrometry in linear positive mode (mass range m/z 2000–20,000 Da). For quality assurance the experiment was repeated with a set of brown trouts (n = 100) originating from the same fish farm and with brown trouts (n = 100) originating from a different fish farm. For specificity testing rainbow trouts (n = 10) were examined accordingly. All obtained mass spectra were processed by means of MALDI Biotyper OC 3.1 and ClinProTools 3.0 software.\r\nThe MALDI Biotyper approach showed limited applicability for the identification of the time of storage. However, it was suitable to reliably discriminate between the closely related species brown and rainbow trout. Processing by ClinProTools revealed four crucial mass peaks (m/z 2594 Da, m/z 4857 Da, m/z 4879 Da, m/z 4899 Da) which enabled a reliable differentiation between day 0 and 3, 7, 9, 11 (rate of correct identification > 90%) as well as the differentiation between day 3 and 7, 9, 11 (rate of correct identification > 72%). However, this approach showed limited applicability within the end of the tested period of storage when comparing between day 7, 9, or 11."}],"type":"scientific_journal_article","publisher":"Elsevier ","issue":"10","quality_controlled":"1","date_updated":"2025-06-18T12:06:11Z","doi":"10.1016/j.foodcont.2017.05.005","publication":"Food Control","_id":"12975","publication_identifier":{"issn":["0956-7135"]},"intvolume":"        80","author":[{"orcid":"0000-0002-4511-9537","first_name":"Sebastian","last_name":"Ulrich","id":"85847","full_name":"Ulrich, Sebastian"},{"full_name":"Beindorf, Philipp–Michael","last_name":"Beindorf","first_name":"Philipp–Michael"},{"full_name":"Biermaier, Barbara","last_name":"Biermaier","first_name":"Barbara"},{"first_name":"Karin","full_name":"Schwaiger, Karin","last_name":"Schwaiger"},{"first_name":"Manfred","last_name":"Gareis","full_name":"Gareis, Manfred"},{"first_name":"Christoph","full_name":"Gottschalk, Christoph","last_name":"Gottschalk"}],"publication_status":"published","volume":80,"language":[{"iso":"eng"}],"date_created":"2025-06-15T10:29:56Z","citation":{"ufg":"<b>Ulrich, Sebastian u. a.</b>: A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry, in: <i>Food Control</i> 80 (2017), H. 10,  S. 281–289.","chicago-de":"Ulrich, Sebastian, Philipp–Michael Beindorf, Barbara Biermaier, Karin Schwaiger, Manfred Gareis und Christoph Gottschalk. 2017. A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. <i>Food Control</i> 80, Nr. 10: 281–289. doi:<a href=\"https://doi.org/10.1016/j.foodcont.2017.05.005\">10.1016/j.foodcont.2017.05.005</a>, .","short":"S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, C. Gottschalk, Food Control 80 (2017) 281–289.","mla":"Ulrich, Sebastian, et al. “A Novel Approach for the Determination of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry.” <i>Food Control</i>, vol. 80, no. 10, 2017, pp. 281–89, <a href=\"https://doi.org/10.1016/j.foodcont.2017.05.005\">https://doi.org/10.1016/j.foodcont.2017.05.005</a>.","apa":"Ulrich, S., Beindorf, P., Biermaier, B., Schwaiger, K., Gareis, M., &#38; Gottschalk, C. (2017). A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. <i>Food Control</i>, <i>80</i>(10), 281–289. <a href=\"https://doi.org/10.1016/j.foodcont.2017.05.005\">https://doi.org/10.1016/j.foodcont.2017.05.005</a>","havard":"S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, C. Gottschalk, A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry, Food Control. 80 (2017) 281–289.","chicago":"Ulrich, Sebastian, Philipp–Michael Beindorf, Barbara Biermaier, Karin Schwaiger, Manfred Gareis, and Christoph Gottschalk. “A Novel Approach for the Determination of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry.” <i>Food Control</i> 80, no. 10 (2017): 281–89. <a href=\"https://doi.org/10.1016/j.foodcont.2017.05.005\">https://doi.org/10.1016/j.foodcont.2017.05.005</a>.","bjps":"<b>Ulrich S <i>et al.</i></b> (2017) A Novel Approach for the Determination of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry. <i>Food Control</i> <b>80</b>, 281–289.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Beindorf, Philipp–Michael</span> ; <span style=\"font-variant:small-caps;\">Biermaier, Barbara</span> ; <span style=\"font-variant:small-caps;\">Schwaiger, Karin</span> ; <span style=\"font-variant:small-caps;\">Gareis, Manfred</span> ; <span style=\"font-variant:small-caps;\">Gottschalk, Christoph</span>: A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. In: <i>Food Control</i> Bd. 80. Amsterdam, Elsevier  (2017), Nr. 10, S. 281–289","ama":"Ulrich S, Beindorf P, Biermaier B, Schwaiger K, Gareis M, Gottschalk C. A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. <i>Food Control</i>. 2017;80(10):281-289. doi:<a href=\"https://doi.org/10.1016/j.foodcont.2017.05.005\">10.1016/j.foodcont.2017.05.005</a>","van":"Ulrich S, Beindorf P, Biermaier B, Schwaiger K, Gareis M, Gottschalk C. A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry. Food Control. 2017;80(10):281–9.","ieee":"S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, and C. Gottschalk, “A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry,” <i>Food Control</i>, vol. 80, no. 10, pp. 281–289, 2017, doi: <a href=\"https://doi.org/10.1016/j.foodcont.2017.05.005\">10.1016/j.foodcont.2017.05.005</a>."},"place":"Amsterdam","title":"A novel approach for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry"},{"title":"Direct identification of edible insects by MALDI-TOF mass spectrometry","author":[{"orcid":"0000-0002-4511-9537","last_name":"Ulrich","id":"85847","full_name":"Ulrich, Sebastian","first_name":"Sebastian"},{"full_name":"Kühn, Ulrike","last_name":"Kühn","first_name":"Ulrike"},{"full_name":"Biermaier, Barbara","last_name":"Biermaier","first_name":"Barbara"},{"first_name":"Nicolo","full_name":"Piacenza, Nicolo","last_name":"Piacenza"},{"first_name":"Karin","last_name":"Schwaiger","full_name":"Schwaiger, Karin"},{"full_name":"Gottschalk, Christoph","last_name":"Gottschalk","first_name":"Christoph"},{"full_name":"Gareis, Manfred","last_name":"Gareis","first_name":"Manfred"}],"intvolume":"        76","publication_identifier":{"issn":["0956-7135"]},"place":"Amsterdam","citation":{"ama":"Ulrich S, Kühn U, Biermaier B, et al. Direct identification of edible insects by MALDI-TOF mass spectrometry. <i>Food Control</i>. 2017;76(6):96-101. doi:<a href=\"https://doi.org/10.1016/j.foodcont.2017.01.010\">10.1016/j.foodcont.2017.01.010</a>","van":"Ulrich S, Kühn U, Biermaier B, Piacenza N, Schwaiger K, Gottschalk C, et al. Direct identification of edible insects by MALDI-TOF mass spectrometry. Food Control. 2017;76(6):96–101.","ieee":"S. Ulrich <i>et al.</i>, “Direct identification of edible insects by MALDI-TOF mass spectrometry,” <i>Food Control</i>, vol. 76, no. 6, pp. 96–101, 2017, doi: <a href=\"https://doi.org/10.1016/j.foodcont.2017.01.010\">10.1016/j.foodcont.2017.01.010</a>.","chicago":"Ulrich, Sebastian, Ulrike Kühn, Barbara Biermaier, Nicolo Piacenza, Karin Schwaiger, Christoph Gottschalk, and Manfred Gareis. “Direct Identification of Edible Insects by MALDI-TOF Mass Spectrometry.” <i>Food Control</i> 76, no. 6 (2017): 96–101. <a href=\"https://doi.org/10.1016/j.foodcont.2017.01.010\">https://doi.org/10.1016/j.foodcont.2017.01.010</a>.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Kühn, Ulrike</span> ; <span style=\"font-variant:small-caps;\">Biermaier, Barbara</span> ; <span style=\"font-variant:small-caps;\">Piacenza, Nicolo</span> ; <span style=\"font-variant:small-caps;\">Schwaiger, Karin</span> ; <span style=\"font-variant:small-caps;\">Gottschalk, Christoph</span> ; <span style=\"font-variant:small-caps;\">Gareis, Manfred</span>: Direct identification of edible insects by MALDI-TOF mass spectrometry. In: <i>Food Control</i> Bd. 76. Amsterdam, Elsevier (2017), Nr. 6, S. 96–101","bjps":"<b>Ulrich S <i>et al.</i></b> (2017) Direct Identification of Edible Insects by MALDI-TOF Mass Spectrometry. <i>Food Control</i> <b>76</b>, 96–101.","havard":"S. Ulrich, U. Kühn, B. Biermaier, N. Piacenza, K. Schwaiger, C. Gottschalk, M. Gareis, Direct identification of edible insects by MALDI-TOF mass spectrometry, Food Control. 76 (2017) 96–101.","chicago-de":"Ulrich, Sebastian, Ulrike Kühn, Barbara Biermaier, Nicolo Piacenza, Karin Schwaiger, Christoph Gottschalk und Manfred Gareis. 2017. Direct identification of edible insects by MALDI-TOF mass spectrometry. <i>Food Control</i> 76, Nr. 6: 96–101. doi:<a href=\"https://doi.org/10.1016/j.foodcont.2017.01.010\">10.1016/j.foodcont.2017.01.010</a>, .","ufg":"<b>Ulrich, Sebastian u. a.</b>: Direct identification of edible insects by MALDI-TOF mass spectrometry, in: <i>Food Control</i> 76 (2017), H. 6,  S. 96–101.","apa":"Ulrich, S., Kühn, U., Biermaier, B., Piacenza, N., Schwaiger, K., Gottschalk, C., &#38; Gareis, M. (2017). Direct identification of edible insects by MALDI-TOF mass spectrometry. <i>Food Control</i>, <i>76</i>(6), 96–101. <a href=\"https://doi.org/10.1016/j.foodcont.2017.01.010\">https://doi.org/10.1016/j.foodcont.2017.01.010</a>","mla":"Ulrich, Sebastian, et al. “Direct Identification of Edible Insects by MALDI-TOF Mass Spectrometry.” <i>Food Control</i>, vol. 76, no. 6, 2017, pp. 96–101, <a href=\"https://doi.org/10.1016/j.foodcont.2017.01.010\">https://doi.org/10.1016/j.foodcont.2017.01.010</a>.","short":"S. Ulrich, U. Kühn, B. Biermaier, N. Piacenza, K. Schwaiger, C. Gottschalk, M. Gareis, Food Control 76 (2017) 96–101."},"language":[{"iso":"eng"}],"date_created":"2025-06-15T10:31:37Z","publication_status":"published","volume":76,"_id":"12976","publication":"Food Control","issue":"6","doi":"10.1016/j.foodcont.2017.01.010","date_updated":"2025-06-18T12:08:47Z","quality_controlled":"1","type":"scientific_journal_article","publisher":"Elsevier","extern":"1","keyword":["MALDI-TOF MS","Mass spectrometry","Edible insects","Authenticity","Food quality"],"abstract":[{"text":"The consumption of edible insects (entomophagy) will gain greater significance facing the increasing global population, which is suggested to reach 9 billion people in 2050 (FAO., 2009). Due to their high amount of proteins, fatty acids, vitamins, and minerals insects represent a valuable source of essential nutrients.\r\nWhile the consumption of insects is very common in many countries of Africa and Asia, there is a far smaller acceptance for entomophagy in Western cultures. Though, products such as noodles or burger paddies made from insect meal have a better compliance and can already be purchased in some countries of the European Union. This processing step however involves the risk of adulteration, because there is no more possibility to authenticate the insects once they are ground.\r\nThe aim of this study was to investigate whether edible insects could be measured and distinguished by MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry). Therefore, different kinds of edible insects (buffalo worms, mealworms, crickets and grasshoppers) were purchased via online shops and ground subsequently. The insect powder was extracted by vigorously shaking in diluted formic acid and measured by MALDI-TOF MS. The measurement provided reproducible as well as specific mass spectra and enabled a precise differentiation of the different species.","lang":"eng"}],"page":"96-101","user_id":"83781","year":"2017","department":[{"_id":"DEP4010"}],"status":"public"},{"abstract":[{"lang":"eng","text":"Stachybotrys (S.) spp. are omnipresent cellulolytic molds. Some species are highly toxic owing to their ability to synthesize various secondary metabolites such as macrocyclic trichothecenes or hemolysins. The reliable identification of Stachybotrys at species level is currently limited to genome-based identification. This study aimed to establish a fast and reliable MALDI-TOF MS identification method by optimizing the pre-analytical steps for protein extraction for subsequent generation of high-quality fingerprint mass spectra. Eight reference strains of the American Type Culture Collection and the Technical University of Denmark were cultivated in triplicate (biological repetitions) for 2 days in malt extract broth. The mycelia (1.5 ml) were first washed with 75 % ethanol and an additional washing step with dimethyl sulfoxide (10 %) was added to remove unspecific low weight masses. Furthermore, mycelia were broken with roughened glass beads in formic acid (70 %) and acetonitrile. The method was successfully applied to a total of 45 isolates of Stachybotrys originating from three different habitats (indoor, feed, and food samples; n = 15 each): Twenty-seven isolates of S. chartarum and 18 isolates of S. chlorohalonata could be identified by MALDI-TOF MS. The data obtained exactly matched those obtained by genome-based identification. The mean score values for S. chartarum ranged from 2.509 to 2.739 and from 2.148 to 2.622 for S. chlorohalonata with a very good reproducibility: the relative standard deviations were between 0.3 % and 6.8 %. Thus, MALDI-TOF MS proved to be a fast and reliable alternative to identification of Stachybotrys spp. by nucleotide amplification and sequencing."}],"page":"7565-7581","extern":"1","keyword":["Stachybotrys spp","MALDI-TOF MS","Mass spectrometry","Filamentous fungi"],"status":"public","year":"2016","user_id":"83781","department":[{"_id":"DEP4010"}],"doi":"10.1007/s00216-016-9800-9","date_updated":"2025-06-18T12:11:46Z","quality_controlled":"1","issue":"27","publisher":"Springer","type":"scientific_journal_article","_id":"12977","publication":"  Analytical & bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry, Analusis and Quimica analitica","title":"Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry","place":"Berlin ; Heidelberg","citation":{"havard":"S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier, B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry,   Analytical &#38; Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica. 408 (2016) 7565–7581.","short":"S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier, B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk,   Analytical &#38; Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica 408 (2016) 7565–7581.","apa":"Ulrich, S., Biermaier, B., Bader, O., Wolf, G., Straubinger, R. K., Didier, A., Sperner, B., Schwaiger, K., Gareis, M., &#38; Gottschalk, C. (2016). Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. <i>  Analytical &#38; Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica</i>, <i>408</i>(27), 7565–7581. <a href=\"https://doi.org/10.1007/s00216-016-9800-9\">https://doi.org/10.1007/s00216-016-9800-9</a>","mla":"Ulrich, Sebastian, et al. “Identification of Stachybotrys Spp. by MALDI-TOF Mass Spectrometry.” <i>  Analytical &#38; Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica</i>, vol. 408, no. 27, 2016, pp. 7565–81, <a href=\"https://doi.org/10.1007/s00216-016-9800-9\">https://doi.org/10.1007/s00216-016-9800-9</a>.","ufg":"<b>Ulrich, Sebastian u. a.</b>: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry, in: <i>  Analytical &#38; bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i> 408 (2016), H. 27,  S. 7565–7581.","chicago-de":"Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis und Christoph Gottschalk. 2016. Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. <i>  Analytical &#38; bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i> 408, Nr. 27: 7565–7581. doi:<a href=\"https://doi.org/10.1007/s00216-016-9800-9\">10.1007/s00216-016-9800-9</a>, .","ieee":"S. Ulrich <i>et al.</i>, “Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry,” <i>  Analytical &#38; bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i>, vol. 408, no. 27, pp. 7565–7581, 2016, doi: <a href=\"https://doi.org/10.1007/s00216-016-9800-9\">10.1007/s00216-016-9800-9</a>.","van":"Ulrich S, Biermaier B, Bader O, Wolf G, Straubinger RK, Didier A, et al. Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry.   Analytical &#38; bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica. 2016;408(27):7565–81.","ama":"Ulrich S, Biermaier B, Bader O, et al. Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. <i>  Analytical &#38; bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i>. 2016;408(27):7565-7581. doi:<a href=\"https://doi.org/10.1007/s00216-016-9800-9\">10.1007/s00216-016-9800-9</a>","bjps":"<b>Ulrich S <i>et al.</i></b> (2016) Identification of Stachybotrys Spp. by MALDI-TOF Mass Spectrometry. <i>  Analytical &#38; bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i> <b>408</b>, 7565–7581.","din1505-2-1":"<span style=\"font-variant:small-caps;\"><span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Biermaier, Barbara</span> ; <span style=\"font-variant:small-caps;\">Bader, Oliver</span> ; <span style=\"font-variant:small-caps;\">Wolf, Georg</span> ; <span style=\"font-variant:small-caps;\">Straubinger, Reinhard K.</span> ; <span style=\"font-variant:small-caps;\">Didier, Andrea</span> ; <span style=\"font-variant:small-caps;\">Sperner, Brigitte</span> ; <span style=\"font-variant:small-caps;\">Schwaiger, Karin</span> ; u. a.</span>: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry. In: <i>  Analytical &#38; bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i> Bd. 408. Berlin ; Heidelberg, Springer (2016), Nr. 27, S. 7565–7581","chicago":"Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis, and Christoph Gottschalk. “Identification of Stachybotrys Spp. by MALDI-TOF Mass Spectrometry.” <i>  Analytical &#38; Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica</i> 408, no. 27 (2016): 7565–81. <a href=\"https://doi.org/10.1007/s00216-016-9800-9\">https://doi.org/10.1007/s00216-016-9800-9</a>."},"date_created":"2025-06-15T10:32:52Z","language":[{"iso":"eng"}],"publication_status":"published","volume":408,"intvolume":"       408","author":[{"orcid":"0000-0002-4511-9537","first_name":"Sebastian","full_name":"Ulrich, Sebastian","last_name":"Ulrich","id":"85847"},{"full_name":"Biermaier, Barbara","last_name":"Biermaier","first_name":"Barbara"},{"first_name":"Oliver","last_name":"Bader","full_name":"Bader, Oliver"},{"last_name":"Wolf","full_name":"Wolf, Georg","first_name":"Georg"},{"first_name":"Reinhard K.","full_name":"Straubinger, Reinhard K.","last_name":"Straubinger"},{"last_name":"Didier","full_name":"Didier, Andrea","first_name":"Andrea"},{"first_name":"Brigitte","full_name":"Sperner, Brigitte","last_name":"Sperner"},{"first_name":"Karin","full_name":"Schwaiger, Karin","last_name":"Schwaiger"},{"last_name":"Gareis","full_name":"Gareis, Manfred","first_name":"Manfred"},{"first_name":"Christoph","full_name":"Gottschalk, Christoph","last_name":"Gottschalk"}],"publication_identifier":{"issn":["1618-2642"],"eissn":["1618-2650"]}}]