---
_id: '7986'
abstract:
- lang: eng
  text: Bioprocess development and optimization are still cost- and time-intensive
    due to the enormous number of experiments involved. In this study, the recently
    introduced model-assisted Design of Experiments (mDoE) concept (Möller et al.
    in Bioproc Biosyst Eng 42(5):867, https://doi.org/10.1007/s00449-019-02089-7,
    2019) was extended and implemented into a software (“mDoE-toolbox”) to significantly
    reduce the number of required cultivations. The application of the toolbox is
    exemplary shown in two case studies with Saccharomyces cerevisiae. In the first
    case study, a fed-batch process was optimized with respect to the pH value and
    linearly rising feeding rates of glucose and nitrogen source. Using the mDoE-toolbox,
    the biomass concentration was increased by 30% compared to previously performed
    experiments. The second case study was the whole-cell biocatalysis of ethyl acetoacetate
    (EAA) to (S)-ethyl-3-hydroxybutyrate (E3HB), for which the feeding rates of glucose,
    nitrogen source, and EAA were optimized. An increase of 80% compared to a previously
    performed experiment with similar initial conditions was achieved for the E3HB
    concentration.
author:
- first_name: André
  full_name: Moser, André
  last_name: Moser
- first_name: Kim B.
  full_name: Kuchemüller, Kim B.
  last_name: Kuchemüller
- first_name: Sahar
  full_name: Deppe, Sahar
  last_name: Deppe
- first_name: Tanja
  full_name: Hernández Rodriguez, Tanja
  id: '52466'
  last_name: Hernández Rodriguez
- first_name: Björn
  full_name: Frahm, Björn
  id: '45666'
  last_name: Frahm
- first_name: Ralf
  full_name: Pörtner, Ralf
  last_name: Pörtner
- first_name: Volker C.
  full_name: Hass, Volker C.
  last_name: Hass
- first_name: Johannes
  full_name: Möller, Johannes
  last_name: Möller
citation:
  ama: 'Moser A, Kuchemüller KB, Deppe S, et al. Model-assisted DoE software: Optimization
    of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses. <i>Bioprocess
    and Biosystems Engineering</i>. 2021;44(4):683-700. doi:<a href="https://doi.org/10.1007/s00449-020-02478-3">10.1007/s00449-020-02478-3</a>'
  apa: 'Moser, A., Kuchemüller, K. B., Deppe, S., Hernández Rodriguez, T., Frahm,
    B., Pörtner, R., Hass, V. C., &#38; Möller, J. (2021). Model-assisted DoE software:
    Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses.
    <i>Bioprocess and Biosystems Engineering</i>, <i>44</i>(4), 683–700. <a href="https://doi.org/10.1007/s00449-020-02478-3">https://doi.org/10.1007/s00449-020-02478-3</a>'
  bjps: '<b>Moser A <i>et al.</i></b> (2021) Model-Assisted DoE Software: Optimization
    of Growth and Biocatalysis in Saccharomyces Cerevisiae Bioprocesses. <i>Bioprocess
    and Biosystems Engineering</i> <b>44</b>, 683–700.'
  chicago: 'Moser, André, Kim B. Kuchemüller, Sahar Deppe, Tanja Hernández Rodriguez,
    Björn Frahm, Ralf Pörtner, Volker C. Hass, and Johannes Möller. “Model-Assisted
    DoE Software: Optimization of Growth and Biocatalysis in Saccharomyces Cerevisiae
    Bioprocesses.” <i>Bioprocess and Biosystems Engineering</i> 44, no. 4 (2021):
    683–700. <a href="https://doi.org/10.1007/s00449-020-02478-3">https://doi.org/10.1007/s00449-020-02478-3</a>.'
  chicago-de: 'Moser, André, Kim B. Kuchemüller, Sahar Deppe, Tanja Hernández Rodriguez,
    Björn Frahm, Ralf Pörtner, Volker C. Hass und Johannes Möller. 2021. Model-assisted
    DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae
    bioprocesses. <i>Bioprocess and Biosystems Engineering</i> 44, Nr. 4: 683–700.
    doi:<a href="https://doi.org/10.1007/s00449-020-02478-3">10.1007/s00449-020-02478-3</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Moser, André</span> ; <span
    style="font-variant:small-caps;">Kuchemüller, Kim B.</span> ; <span style="font-variant:small-caps;">Deppe,
    Sahar</span> ; <span style="font-variant:small-caps;">Hernández Rodriguez, Tanja</span>
    ; <span style="font-variant:small-caps;">Frahm, Björn</span> ; <span style="font-variant:small-caps;">Pörtner,
    Ralf</span> ; <span style="font-variant:small-caps;">Hass, Volker C.</span> ;
    <span style="font-variant:small-caps;">Möller, Johannes</span>: Model-assisted
    DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae
    bioprocesses. In: <i>Bioprocess and Biosystems Engineering</i> Bd. 44. Berlin ;
    Heidelberg [u.a.], Springer (2021), Nr. 4, S. 683–700'
  havard: 'A. Moser, K.B. Kuchemüller, S. Deppe, T. Hernández Rodriguez, B. Frahm,
    R. Pörtner, V.C. Hass, J. Möller, Model-assisted DoE software: Optimization of
    growth and biocatalysis in Saccharomyces cerevisiae bioprocesses, Bioprocess and
    Biosystems Engineering. 44 (2021) 683–700.'
  ieee: 'A. Moser <i>et al.</i>, “Model-assisted DoE software: Optimization of growth
    and biocatalysis in Saccharomyces cerevisiae bioprocesses,” <i>Bioprocess and
    Biosystems Engineering</i>, vol. 44, no. 4, pp. 683–700, 2021, doi: <a href="https://doi.org/10.1007/s00449-020-02478-3">10.1007/s00449-020-02478-3</a>.'
  mla: 'Moser, André, et al. “Model-Assisted DoE Software: Optimization of Growth
    and Biocatalysis in Saccharomyces Cerevisiae Bioprocesses.” <i>Bioprocess and
    Biosystems Engineering</i>, vol. 44, no. 4, 2021, pp. 683–700, <a href="https://doi.org/10.1007/s00449-020-02478-3">https://doi.org/10.1007/s00449-020-02478-3</a>.'
  short: A. Moser, K.B. Kuchemüller, S. Deppe, T. Hernández Rodriguez, B. Frahm, R.
    Pörtner, V.C. Hass, J. Möller, Bioprocess and Biosystems Engineering 44 (2021)
    683–700.
  ufg: '<b>Moser, André u. a.</b>: Model-assisted DoE software: Optimization of growth
    and biocatalysis in Saccharomyces cerevisiae bioprocesses, in: <i>Bioprocess and
    Biosystems Engineering</i> 44 (2021), H. 4,  S. 683–700.'
  van: 'Moser A, Kuchemüller KB, Deppe S, Hernández Rodriguez T, Frahm B, Pörtner
    R, et al. Model-assisted DoE software: Optimization of growth and biocatalysis
    in Saccharomyces cerevisiae bioprocesses. Bioprocess and Biosystems Engineering.
    2021;44(4):683–700.'
date_created: 2022-05-05T13:10:11Z
date_updated: 2023-08-21T07:55:05Z
department:
- _id: DEP4021
doi: 10.1007/s00449-020-02478-3
intvolume: '        44'
issue: '4'
keyword:
- Biocatalysis
- Monte Carlo methods
- Fed-batch strategy
- Model-assisted design of experiments
- Quality by design
language:
- iso: eng
page: 683-700
place: Berlin ; Heidelberg [u.a.]
publication: Bioprocess and Biosystems Engineering
publication_identifier:
  eissn:
  - 1615-7605
  isbn:
  - 1615-7591
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: 'Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces
  cerevisiae bioprocesses'
type: scientific_journal_article
user_id: '83781'
volume: 44
year: '2021'
...