---
_id: '12953'
abstract:
- lang: eng
  text: Starch gelatinization is a major determinant of carbohydrate digestibility
    and varies with diet processing. Laboratory rodent diets are often marketed as
    identical, but are sold in different forms, regardless of the markedly higher
    starch gelatinization in extruded than in pelleted diets. Our hypothesis was that
    this would impact energy and nutrient digestibility in mice fed pellets or extrudate,
    respectively. Trial 1 showed that feeding C57BL/6 mice a standard maintenance
    diet in extruded form results in a significantly higher digestibility of organic
    matter, energy, and carbohydrates than the identical diet in pelleted form. The
    replication of the experiment, however, revealed a variation between batches of
    the same pelleted diet regarding starch and total dietary fiber contents. Given
    the significant differences in diet digestibility and the potential impacts of
    digestibility on nutrient utilization, the intestinal microbiome, and intermediary
    metabolism, trials performed with differently processed diets are not comparable.
    This might partly explain failures to reproduce results, especially in gastrointestinal
    or microbiome research. Considering this impact on experimental animals, the degree
    of starch gelatinization should be declared in the diet information for laboratory
    animal diets. The differences between batches of laboratory animal diets as observed
    in the pellets are not acceptable.
article_number: '523'
author:
- first_name: Linda F.
  full_name: Böswald, Linda F.
  last_name: Böswald
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Ellen
  full_name: Kienzle, Ellen
  last_name: Kienzle
citation:
  ama: Böswald LF, Wenderlein J, Straubinger RK, Ulrich S, Kienzle E. Processing Matters
    in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility.
    <i>Animals</i>. 2021;11(2). doi:<a href="https://doi.org/10.3390/ani11020523">10.3390/ani11020523</a>
  apa: Böswald, L. F., Wenderlein, J., Straubinger, R. K., Ulrich, S., &#38; Kienzle,
    E. (2021). Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy
    and Nutrient Digestibility. <i>Animals</i>, <i>11</i>(2), Article 523. <a href="https://doi.org/10.3390/ani11020523">https://doi.org/10.3390/ani11020523</a>
  bjps: <b>Böswald LF <i>et al.</i></b> (2021) Processing Matters in Nutrient-Matched
    Laboratory Diets for Mice—Energy and Nutrient Digestibility. <i>Animals</i> <b>11</b>.
  chicago: Böswald, Linda F., Jasmin Wenderlein, Reinhard K. Straubinger, Sebastian
    Ulrich, and Ellen Kienzle. “Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Energy and Nutrient Digestibility.” <i>Animals</i> 11, no. 2 (2021).
    <a href="https://doi.org/10.3390/ani11020523">https://doi.org/10.3390/ani11020523</a>.
  chicago-de: Böswald, Linda F., Jasmin Wenderlein, Reinhard K. Straubinger, Sebastian
    Ulrich und Ellen Kienzle. 2021. Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Energy and Nutrient Digestibility. <i>Animals</i> 11, Nr. 2. doi:<a
    href="https://doi.org/10.3390/ani11020523">10.3390/ani11020523</a>, .
  din1505-2-1: '<span style="font-variant:small-caps;">Böswald, Linda F.</span> ;
    <span style="font-variant:small-caps;">Wenderlein, Jasmin</span> ; <span style="font-variant:small-caps;">Straubinger,
    Reinhard K.</span> ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>
    ; <span style="font-variant:small-caps;">Kienzle, Ellen</span>: Processing Matters
    in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility.
    In: <i>Animals</i> Bd. 11. Basel, MDPI (2021), Nr. 2'
  havard: L.F. Böswald, J. Wenderlein, R.K. Straubinger, S. Ulrich, E. Kienzle, Processing
    Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility,
    Animals. 11 (2021).
  ieee: 'L. F. Böswald, J. Wenderlein, R. K. Straubinger, S. Ulrich, and E. Kienzle,
    “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient
    Digestibility,” <i>Animals</i>, vol. 11, no. 2, Art. no. 523, 2021, doi: <a href="https://doi.org/10.3390/ani11020523">10.3390/ani11020523</a>.'
  mla: Böswald, Linda F., et al. “Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Energy and Nutrient Digestibility.” <i>Animals</i>, vol. 11, no.
    2, 523, 2021, <a href="https://doi.org/10.3390/ani11020523">https://doi.org/10.3390/ani11020523</a>.
  short: L.F. Böswald, J. Wenderlein, R.K. Straubinger, S. Ulrich, E. Kienzle, Animals
    11 (2021).
  ufg: '<b>Böswald, Linda F. u. a.</b>: Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Energy and Nutrient Digestibility, in: <i>Animals</i> 11 (2021),
    H. 2.'
  van: Böswald LF, Wenderlein J, Straubinger RK, Ulrich S, Kienzle E. Processing Matters
    in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility.
    Animals. 2021;11(2).
date_created: 2025-06-15T10:00:16Z
date_updated: 2025-06-16T12:27:09Z
department:
- _id: DEP4010
doi: 10.3390/ani11020523
extern: '1'
intvolume: '        11'
issue: '2'
keyword:
- standardization
- carbohydrate digestibility
- feed processing
- starch gelatinization
- gut
language:
- iso: eng
place: Basel
publication: Animals
publication_identifier:
  eissn:
  - 2076-2615
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and
  Nutrient Digestibility
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2021'
...