[{"title":"Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks","date_created":"2025-06-15T09:53:01Z","language":[{"iso":"eng"}],"publication_status":"published","volume":24,"place":"Basel","citation":{"ufg":"<b>Wiesinger, Anna u. a.</b>: Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks, in: <i>International Journal of Molecular Sciences</i> 24 (2023), H. 2.","chicago-de":"Wiesinger, Anna, Jasmin Wenderlein, Sebastian Ulrich, Stephanie Hiereth, Lidia Chitimia-Dobler und Reinhard K. Straubinger. 2023. Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks. <i>International Journal of Molecular Sciences</i> 24, Nr. 2. doi:<a href=\"https://doi.org/10.3390/ijms24021100\">10.3390/ijms24021100</a>, .","short":"A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler, R.K. Straubinger, International Journal of Molecular Sciences 24 (2023).","mla":"Wiesinger, Anna, et al. “Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks.” <i>International Journal of Molecular Sciences</i>, vol. 24, no. 2, 1100, 2023, <a href=\"https://doi.org/10.3390/ijms24021100\">https://doi.org/10.3390/ijms24021100</a>.","apa":"Wiesinger, A., Wenderlein, J., Ulrich, S., Hiereth, S., Chitimia-Dobler, L., &#38; Straubinger, R. K. (2023). Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks. <i>International Journal of Molecular Sciences</i>, <i>24</i>(2), Article 1100. <a href=\"https://doi.org/10.3390/ijms24021100\">https://doi.org/10.3390/ijms24021100</a>","havard":"A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler, R.K. Straubinger, Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks, International Journal of Molecular Sciences. 24 (2023).","chicago":"Wiesinger, Anna, Jasmin Wenderlein, Sebastian Ulrich, Stephanie Hiereth, Lidia Chitimia-Dobler, and Reinhard K. Straubinger. “Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks.” <i>International Journal of Molecular Sciences</i> 24, no. 2 (2023). <a href=\"https://doi.org/10.3390/ijms24021100\">https://doi.org/10.3390/ijms24021100</a>.","bjps":"<b>Wiesinger A <i>et al.</i></b> (2023) Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks. <i>International Journal of Molecular Sciences</i> <b>24</b>.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Wiesinger, Anna</span> ; <span style=\"font-variant:small-caps;\">Wenderlein, Jasmin</span> ; <span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Hiereth, Stephanie</span> ; <span style=\"font-variant:small-caps;\">Chitimia-Dobler, Lidia</span> ; <span style=\"font-variant:small-caps;\">Straubinger, Reinhard K.</span>: Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks. In: <i>International Journal of Molecular Sciences</i> Bd. 24. Basel, MDPI AG (2023), Nr. 2","ama":"Wiesinger A, Wenderlein J, Ulrich S, Hiereth S, Chitimia-Dobler L, Straubinger RK. Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks. <i>International Journal of Molecular Sciences</i>. 2023;24(2). doi:<a href=\"https://doi.org/10.3390/ijms24021100\">10.3390/ijms24021100</a>","van":"Wiesinger A, Wenderlein J, Ulrich S, Hiereth S, Chitimia-Dobler L, Straubinger RK. Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks. International Journal of Molecular Sciences. 2023;24(2).","ieee":"A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler, and R. K. Straubinger, “Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks,” <i>International Journal of Molecular Sciences</i>, vol. 24, no. 2, Art. no. 1100, 2023, doi: <a href=\"https://doi.org/10.3390/ijms24021100\">10.3390/ijms24021100</a>."},"publication_identifier":{"eissn":["1422-0067"],"issn":["1661-6596"]},"intvolume":"        24","author":[{"first_name":"Anna","last_name":"Wiesinger","full_name":"Wiesinger, Anna"},{"full_name":"Wenderlein, Jasmin","last_name":"Wenderlein","first_name":"Jasmin"},{"orcid":"0000-0002-4511-9537","id":"85847","last_name":"Ulrich","full_name":"Ulrich, Sebastian","first_name":"Sebastian"},{"last_name":"Hiereth","full_name":"Hiereth, Stephanie","first_name":"Stephanie"},{"last_name":"Chitimia-Dobler","full_name":"Chitimia-Dobler, Lidia","first_name":"Lidia"},{"last_name":"Straubinger","full_name":"Straubinger, Reinhard K.","first_name":"Reinhard K."}],"article_number":"1100","_id":"12942","publication":"International Journal of Molecular Sciences","quality_controlled":"1","doi":"10.3390/ijms24021100","date_updated":"2025-06-16T09:25:29Z","issue":"2","publisher":"MDPI AG","type":"scientific_journal_article","abstract":[{"text":"The ectoparasite Ixodes ricinus is an important vector for many tick-borne diseases (TBD) in the northern hemisphere, such as Lyme borreliosis, rickettsiosis, human granulocytic anaplasmosis, or tick-borne encephalitis virus. As climate change will lead to rising temperatures in the next years, we expect an increase in tick activity, tick population, and thus in the spread of TBD. Consequently, it has never been more critical to understand relationships within the microbial communities in ticks that might contribute to the tick’s fitness and the occurrence of TBD. Therefore, we analyzed the microbiota in different tick tissues such as midgut, salivary glands, and residual tick material, as well as the microbiota in complete Ixodes ricinus ticks using 16S rRNA gene amplicon sequencing. By using a newly developed DNA extraction protocol for tick tissue samples and a self-designed mock community, we were able to detect endosymbionts and pathogens that have been described in the literature previously. Further, this study displayed the usefulness of including a mock community during bioinformatic analysis to identify essential bacteria within the tick.","lang":"eng"}],"keyword":["Ixodes ricinus","microbiome","tick-borne disease","salivary glands","midgut","endosymbiont","Candidatus Midichloria mitochondrii"],"extern":"1","status":"public","department":[{"_id":"DEP4010"}],"year":"2023","user_id":"83781"},{"title":"The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny","citation":{"havard":"K. Keiz, S. Ulrich, J. Wenderlein, P. Keferloher, A. Wiesinger, K. Neuhaus, I. Lagkouvardos, H. Wedekind, R.K. Straubinger, The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny, Microorganisms : Open Access Journal . 11 (2023).","ufg":"<b>Keiz, Katharina u. a.</b>: The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny, in: <i>Microorganisms : open access journal </i> 11 (2023), H. 1.","chicago-de":"Keiz, Katharina, Sebastian Ulrich, Jasmin Wenderlein, Patrick Keferloher, Anna Wiesinger, Klaus Neuhaus, Ilias Lagkouvardos, Helmut Wedekind und Reinhard K. Straubinger. 2023. The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny. <i>Microorganisms : open access journal </i> 11, Nr. 1. doi:<a href=\"https://doi.org/10.3390/microorganisms11010211\">10.3390/microorganisms11010211</a>, .","short":"K. Keiz, S. Ulrich, J. Wenderlein, P. Keferloher, A. Wiesinger, K. Neuhaus, I. Lagkouvardos, H. Wedekind, R.K. Straubinger, Microorganisms : Open Access Journal  11 (2023).","apa":"Keiz, K., Ulrich, S., Wenderlein, J., Keferloher, P., Wiesinger, A., Neuhaus, K., Lagkouvardos, I., Wedekind, H., &#38; Straubinger, R. K. (2023). The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny. <i>Microorganisms : Open Access Journal </i>, <i>11</i>(1), Article 211. <a href=\"https://doi.org/10.3390/microorganisms11010211\">https://doi.org/10.3390/microorganisms11010211</a>","mla":"Keiz, Katharina, et al. “The Development of the Bacterial Community of Brown Trout (Salmo Trutta) during Ontogeny.” <i>Microorganisms : Open Access Journal </i>, vol. 11, no. 1, 211, 2023, <a href=\"https://doi.org/10.3390/microorganisms11010211\">https://doi.org/10.3390/microorganisms11010211</a>.","ama":"Keiz K, Ulrich S, Wenderlein J, et al. The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny. <i>Microorganisms : open access journal </i>. 2023;11(1). doi:<a href=\"https://doi.org/10.3390/microorganisms11010211\">10.3390/microorganisms11010211</a>","van":"Keiz K, Ulrich S, Wenderlein J, Keferloher P, Wiesinger A, Neuhaus K, et al. The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny. Microorganisms : open access journal . 2023;11(1).","ieee":"K. Keiz <i>et al.</i>, “The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny,” <i>Microorganisms : open access journal </i>, vol. 11, no. 1, Art. no. 211, 2023, doi: <a href=\"https://doi.org/10.3390/microorganisms11010211\">10.3390/microorganisms11010211</a>.","chicago":"Keiz, Katharina, Sebastian Ulrich, Jasmin Wenderlein, Patrick Keferloher, Anna Wiesinger, Klaus Neuhaus, Ilias Lagkouvardos, Helmut Wedekind, and Reinhard K. Straubinger. “The Development of the Bacterial Community of Brown Trout (Salmo Trutta) during Ontogeny.” <i>Microorganisms : Open Access Journal </i> 11, no. 1 (2023). <a href=\"https://doi.org/10.3390/microorganisms11010211\">https://doi.org/10.3390/microorganisms11010211</a>.","bjps":"<b>Keiz K <i>et al.</i></b> (2023) The Development of the Bacterial Community of Brown Trout (Salmo Trutta) during Ontogeny. <i>Microorganisms : open access journal </i> <b>11</b>.","din1505-2-1":"<span style=\"font-variant:small-caps;\"><span style=\"font-variant:small-caps;\">Keiz, Katharina</span> ; <span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Wenderlein, Jasmin</span> ; <span style=\"font-variant:small-caps;\">Keferloher, Patrick</span> ; <span style=\"font-variant:small-caps;\">Wiesinger, Anna</span> ; <span style=\"font-variant:small-caps;\">Neuhaus, Klaus</span> ; <span style=\"font-variant:small-caps;\">Lagkouvardos, Ilias</span> ; <span style=\"font-variant:small-caps;\">Wedekind, Helmut</span> ; u. a.</span>: The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny. In: <i>Microorganisms : open access journal </i> Bd. 11. Basel, MDPI AG (2023), Nr. 1"},"place":"Basel","volume":11,"publication_status":"published","language":[{"iso":"eng"}],"date_created":"2025-06-15T09:53:31Z","author":[{"first_name":"Katharina","last_name":"Keiz","full_name":"Keiz, Katharina"},{"orcid":"0000-0002-4511-9537","id":"85847","last_name":"Ulrich","full_name":"Ulrich, Sebastian","first_name":"Sebastian"},{"first_name":"Jasmin","full_name":"Wenderlein, Jasmin","last_name":"Wenderlein"},{"first_name":"Patrick","last_name":"Keferloher","full_name":"Keferloher, Patrick"},{"first_name":"Anna","last_name":"Wiesinger","full_name":"Wiesinger, Anna"},{"full_name":"Neuhaus, Klaus","last_name":"Neuhaus","first_name":"Klaus"},{"first_name":"Ilias","last_name":"Lagkouvardos","full_name":"Lagkouvardos, Ilias"},{"first_name":"Helmut","full_name":"Wedekind, Helmut","last_name":"Wedekind"},{"full_name":"Straubinger, Reinhard K.","last_name":"Straubinger","first_name":"Reinhard K."}],"intvolume":"        11","publication_identifier":{"eissn":["2076-2607"]},"article_number":"211","_id":"12943","publication":"Microorganisms : open access journal ","date_updated":"2025-06-16T11:09:43Z","doi":"10.3390/microorganisms11010211","quality_controlled":"1","issue":"1","publisher":"MDPI AG","type":"scientific_journal_article","abstract":[{"lang":"eng","text":"Brown trout (Salmo trutta) is an important aquaculture species in Germany, but its production faces challenges due to global warming and a high embryo mortality. Climate factors might influence the fish’s bacterial community (BC) and thus increase embryo mortality. Yet, knowledge of the physiological BC during ontogeny in general is scarce. In this project, the BC of brown trout has been investigated in a period from unfertilized egg to 95 days post fertilization (dpf) using 16S rRNA gene amplicon sequencing. Developmental changes differed between early and late ontogeny and major differences in BC occurred especially during early developmental stages. Thus, analysis was conducted separately for 0 to 67 dpf and from 67 to 95 dpf. All analyzed stages were sampled in toto to avoid bias due to different sampling methods in different developmental stages. The most abundant phylum in the BC of all developmental stages was Pseudomonadota, while only two families (Comamonadaceae and Moraxellaceae) occurred in all developmental stages. The early developmental stages until 67 dpf displayed greater shifts in their BC regarding bacterial richness, microbial diversity, and taxonomic composition. Thereafter, in the fry stages, the BC seemed to stabilize and changes were moderate. In future studies, a reduction in the sampling time frames during early development, an increase in sampling numbers, and an attempt for biological reproduction in order to characterize the causes of these variations is recommended."}],"extern":"1","keyword":["fish","brown trout","Salmo trutta","development","ontogeny","microbiome","bacterial community"],"status":"public","user_id":"83781","year":"2023","department":[{"_id":"DEP4010"}]},{"publication":"Animals","article_number":"862","_id":"12950","citation":{"ufg":"<b>Wenderlein, Jasmin u. a.</b>: Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome, in: <i>Animals</i> 11 (2021), H. 3.","chicago":"Wenderlein, Jasmin, Linda F. Böswald, Sebastian Ulrich, Ellen Kienzle, Klaus Neuhaus, Ilias Lagkouvardos, Christian Zenner, and Reinhard K. Straubinger. “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome.” <i>Animals</i> 11, no. 3 (2021). <a href=\"https://doi.org/10.3390/ani11030862\">https://doi.org/10.3390/ani11030862</a>.","chicago-de":"Wenderlein, Jasmin, Linda F. Böswald, Sebastian Ulrich, Ellen Kienzle, Klaus Neuhaus, Ilias Lagkouvardos, Christian Zenner und Reinhard K. Straubinger. 2021. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome. <i>Animals</i> 11, Nr. 3. doi:<a href=\"https://doi.org/10.3390/ani11030862\">10.3390/ani11030862</a>, .","bjps":"<b>Wenderlein J <i>et al.</i></b> (2021) Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome. <i>Animals</i> <b>11</b>.","short":"J. Wenderlein, L.F. Böswald, S. Ulrich, E. Kienzle, K. Neuhaus, I. Lagkouvardos, C. Zenner, R.K. Straubinger, Animals 11 (2021).","din1505-2-1":"<span style=\"font-variant:small-caps;\">Wenderlein, Jasmin</span> ; <span style=\"font-variant:small-caps;\">Böswald, Linda F.</span> ; <span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Kienzle, Ellen</span> ; <span style=\"font-variant:small-caps;\">Neuhaus, Klaus</span> ; <span style=\"font-variant:small-caps;\">Lagkouvardos, Ilias</span> ; <span style=\"font-variant:small-caps;\">Zenner, Christian</span> ; <span style=\"font-variant:small-caps;\">Straubinger, Reinhard K.</span>: Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome. In: <i>Animals</i> Bd. 11. Basel, MDPI AG (2021), Nr. 3","mla":"Wenderlein, Jasmin, et al. “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome.” <i>Animals</i>, vol. 11, no. 3, 862, 2021, <a href=\"https://doi.org/10.3390/ani11030862\">https://doi.org/10.3390/ani11030862</a>.","apa":"Wenderlein, J., Böswald, L. F., Ulrich, S., Kienzle, E., Neuhaus, K., Lagkouvardos, I., Zenner, C., &#38; Straubinger, R. K. (2021). Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome. <i>Animals</i>, <i>11</i>(3), Article 862. <a href=\"https://doi.org/10.3390/ani11030862\">https://doi.org/10.3390/ani11030862</a>","van":"Wenderlein J, Böswald LF, Ulrich S, Kienzle E, Neuhaus K, Lagkouvardos I, et al. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome. Animals. 2021;11(3).","ama":"Wenderlein J, Böswald LF, Ulrich S, et al. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome. <i>Animals</i>. 2021;11(3). doi:<a href=\"https://doi.org/10.3390/ani11030862\">10.3390/ani11030862</a>","ieee":"J. Wenderlein <i>et al.</i>, “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome,” <i>Animals</i>, vol. 11, no. 3, Art. no. 862, 2021, doi: <a href=\"https://doi.org/10.3390/ani11030862\">10.3390/ani11030862</a>.","havard":"J. Wenderlein, L.F. Böswald, S. Ulrich, E. Kienzle, K. Neuhaus, I. Lagkouvardos, C. Zenner, R.K. Straubinger, Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome, Animals. 11 (2021)."},"place":"Basel","publication_status":"published","volume":11,"language":[{"iso":"eng"}],"date_created":"2025-06-15T09:58:59Z","author":[{"first_name":"Jasmin","full_name":"Wenderlein, Jasmin","last_name":"Wenderlein"},{"last_name":"Böswald","full_name":"Böswald, Linda F.","first_name":"Linda F."},{"orcid":"0000-0002-4511-9537","first_name":"Sebastian","id":"85847","last_name":"Ulrich","full_name":"Ulrich, Sebastian"},{"full_name":"Kienzle, Ellen","last_name":"Kienzle","first_name":"Ellen"},{"last_name":"Neuhaus","full_name":"Neuhaus, Klaus","first_name":"Klaus"},{"full_name":"Lagkouvardos, Ilias","last_name":"Lagkouvardos","first_name":"Ilias"},{"full_name":"Zenner, Christian","last_name":"Zenner","first_name":"Christian"},{"first_name":"Reinhard K.","full_name":"Straubinger, Reinhard K.","last_name":"Straubinger"}],"intvolume":"        11","publication_identifier":{"issn":["2076-2615"]},"title":"Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome","status":"public","user_id":"83781","year":"2021","department":[{"_id":"DEP4010"}],"abstract":[{"text":"The composition of the microbiome is subject to the host’s diet. In commercial laboratory mouse diets, different physical forms of the same diets are available, containing—according to their labels—identical ingredients and nutrient compositions. However, variations in nutrient composition and starch gelatinization due to production processes and their impact on digestibility have been described. In this study, a total of 48 C57BL/J6 mice were assigned to two equal groups and were fed diets (produced with different processes—extruded vs. pelleted) for eight weeks in two biological replicates. At the end of the experiment, samples were collected from five different gastrointestinal regions, including the stomach, small intestine, cecum, large intestine, and an extracorporeal region (feces), and the microbiome was analyzed with 16S rRNA gene amplicon sequencing. The replicates in both experiments differed significantly in their relative abundances of Muribaculaceae species. Furthermore, the gastrointestinal content of pellet-fed mice contained larger numbers of Lactobacillus species. These results indicate that starch gelatinization and ingredient composition significantly influence microbial makeup. In conclusion, different feed processing methods may affect fundamental digestive and metabolic processes, impacting animal experiments and biasing microbiome data.","lang":"eng"}],"extern":"1","keyword":["feed processing","starch gelatinization","laboratory mouse","diet","intestinal microbiome"],"publisher":"MDPI AG","type":"scientific_journal_article","date_updated":"2025-06-16T12:07:21Z","doi":"10.3390/ani11030862","quality_controlled":"1","issue":"3"}]