---
_id: '12971'
abstract:
- lang: eng
  text: The genus Stachybotrys produces a broad diversity of secondary metabolites,
    including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although
    the class of the phenylspirodrimanes is the major one and consists of a multitude
    of metabolites bearing various structural modifications, few investigations have
    been carried out. Thus, the presented study deals with the quantitative determination
    of several secondary metabolites produced by distinct Stachybotrys species for
    comparison of their metabolite profiles. For that purpose, 15 of the primarily
    produced secondary metabolites were isolated from fungal cultures and structurally
    characterized in order to be used as analytical standards for the development
    of an LC-MS/MS multimethod. The developed method was applied to the analysis of
    micro-scale extracts from 5 different Stachybotrys strains, which were cultured
    on different media. In that process, spontaneous dialdehyde/lactone isomerization
    was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes
    were quantitatively investigated for the first time. The metabolite profiles of
    Stachybotrys species are considerably influenced by time of growth and substrate
    availability, as well as the individual biosynthetic potential of the respective
    species. Regarding the reported adverse effects associated with Stachybotrys growth
    in building environments, combinatory effects of the investigated secondary metabolites
    should be addressed and the role of the phenylspirodrimanes re-evaluated in future
    research.
article_number: '133'
author:
- first_name: Annika
  full_name: Jagels, Annika
  last_name: Jagels
- first_name: Viktoria
  full_name: Lindemann, Viktoria
  last_name: Lindemann
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Benedikt
  full_name: Cramer, Benedikt
  last_name: Cramer
- first_name: Florian
  full_name: Hübner, Florian
  last_name: Hübner
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
- first_name: Hans-Ulrich
  full_name: Humpf, Hans-Ulrich
  last_name: Humpf
citation:
  ama: Jagels A, Lindemann V, Ulrich S, et al. Exploring Secondary Metabolite Profiles
    of Stachybotrys spp. by LC-MS/MS. <i>Toxins</i>. 2019;11(3). doi:<a href="https://doi.org/10.3390/toxins11030133">10.3390/toxins11030133</a>
  apa: Jagels, A., Lindemann, V., Ulrich, S., Gottschalk, C., Cramer, B., Hübner,
    F., Gareis, M., &#38; Humpf, H.-U. (2019). Exploring Secondary Metabolite Profiles
    of Stachybotrys spp. by LC-MS/MS. <i>Toxins</i>, <i>11</i>(3), Article 133. <a
    href="https://doi.org/10.3390/toxins11030133">https://doi.org/10.3390/toxins11030133</a>
  bjps: <b>Jagels A <i>et al.</i></b> (2019) Exploring Secondary Metabolite Profiles
    of Stachybotrys Spp. by LC-MS/MS. <i>Toxins</i> <b>11</b>.
  chicago: Jagels, Annika, Viktoria Lindemann, Sebastian Ulrich, Christoph Gottschalk,
    Benedikt Cramer, Florian Hübner, Manfred Gareis, and Hans-Ulrich Humpf. “Exploring
    Secondary Metabolite Profiles of Stachybotrys Spp. by LC-MS/MS.” <i>Toxins</i>
    11, no. 3 (2019). <a href="https://doi.org/10.3390/toxins11030133">https://doi.org/10.3390/toxins11030133</a>.
  chicago-de: Jagels, Annika, Viktoria Lindemann, Sebastian Ulrich, Christoph Gottschalk,
    Benedikt Cramer, Florian Hübner, Manfred Gareis und Hans-Ulrich Humpf. 2019. Exploring
    Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. <i>Toxins</i>
    11, Nr. 3. doi:<a href="https://doi.org/10.3390/toxins11030133">10.3390/toxins11030133</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Jagels, Annika</span> ; <span
    style="font-variant:small-caps;">Lindemann, Viktoria</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Gottschalk, Christoph</span>
    ; <span style="font-variant:small-caps;">Cramer, Benedikt</span> ; <span style="font-variant:small-caps;">Hübner,
    Florian</span> ; <span style="font-variant:small-caps;">Gareis, Manfred</span>
    ; <span style="font-variant:small-caps;">Humpf, Hans-Ulrich</span>: Exploring
    Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. In: <i>Toxins</i>
    Bd. 11. Basel, MDPI (2019), Nr. 3'
  havard: A. Jagels, V. Lindemann, S. Ulrich, C. Gottschalk, B. Cramer, F. Hübner,
    M. Gareis, H.-U. Humpf, Exploring Secondary Metabolite Profiles of Stachybotrys
    spp. by LC-MS/MS, Toxins. 11 (2019).
  ieee: 'A. Jagels <i>et al.</i>, “Exploring Secondary Metabolite Profiles of Stachybotrys
    spp. by LC-MS/MS,” <i>Toxins</i>, vol. 11, no. 3, Art. no. 133, 2019, doi: <a
    href="https://doi.org/10.3390/toxins11030133">10.3390/toxins11030133</a>.'
  mla: Jagels, Annika, et al. “Exploring Secondary Metabolite Profiles of Stachybotrys
    Spp. by LC-MS/MS.” <i>Toxins</i>, vol. 11, no. 3, 133, 2019, <a href="https://doi.org/10.3390/toxins11030133">https://doi.org/10.3390/toxins11030133</a>.
  short: A. Jagels, V. Lindemann, S. Ulrich, C. Gottschalk, B. Cramer, F. Hübner,
    M. Gareis, H.-U. Humpf, Toxins 11 (2019).
  ufg: '<b>Jagels, Annika u. a.</b>: Exploring Secondary Metabolite Profiles of Stachybotrys
    spp. by LC-MS/MS, in: <i>Toxins</i> 11 (2019), H. 3.'
  van: Jagels A, Lindemann V, Ulrich S, Gottschalk C, Cramer B, Hübner F, et al. Exploring
    Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. Toxins. 2019;11(3).
date_created: 2025-06-15T10:23:36Z
date_updated: 2025-06-17T14:12:55Z
department:
- _id: DEP4010
doi: 10.3390/toxins11030133
extern: '1'
intvolume: '        11'
issue: '3'
keyword:
- Stachybotrys spp.
- metabolite profiles
- LC-MS/MS
- satratoxins
- phenylspirodrimanes
- stachybotrychromenes
- biosynthetic production
language:
- iso: eng
place: Basel
publication: Toxins
publication_identifier:
  eissn:
  - 2072-6651
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2019'
...