---
_id: '13652'
abstract:
- lang: eng
text: "Modellbasierte Konzepte und Simulationstechniken in Kombination mit digitalen
Werkzeugen erweisen sich als Schlüssel, um das volle Potenzial biopharmazeutischer
Produktionsprozesse zu erschließen, die mehrere herausfordernde Entwicklungs-
und Prozessschritte enthalten. Einer dieser Schritte ist der zeit- und kostenintensive
Zellproliferationsprozess (auch als Seed Train bezeichnet), um die Zellzahl vom
Auftauen der Zellen bis zum Produktionsmaßstab zu erhöhen. Herausforderungen wie
komplexer Zellstoffwechsel, Chargen-zu-Chargen-Variationen, Variabilitäten im
Zellverhalten und Einflüsse von Änderungen der Kultivierungsbedingungen erfordern
adäquate digitale Lösungen, um Informationen über den aktuellen und zukünftigen
Prozesszustand bereitzustellen und korrekte Prozessentscheidungen abzuleiten.\r\n\r\nZu
diesem Zweck haben sich digitale Seed Train Zwillinge als effizient erwiesen,
die das zeitabhängige Verhalten wichtiger Prozessvariablen basierend auf mathematischen
Modellen, Strategien und Anpassungsverfahren digital darstellen.\r\n\r\nDieses
Kapitel skizziert die Notwendigkeit der Digitalisierung von Seed Trains, den Aufbau
eines digitalen Seed Train Zwillings, die Rolle der Parameterschätzung und verschiedene
statistische Methoden in diesem Zusammenhang, die auf mehrere Probleme im Bereich
der Bioprozessierung anwendbar sind. Die Ergebnisse einer Fallstudie werden vorgestellt,
um einen Bayes’schen Ansatz zur Parameterschätzung und Vorhersage eines industriellen
Zellkultur-Seed Trains für die Seed Train Digitalisierung zu veranschaulichen."
author:
- first_name: Tanja
full_name: Hernández Rodriguez, Tanja
id: '52466'
last_name: Hernández Rodriguez
- first_name: Björn
full_name: Frahm, Björn
id: '45666'
last_name: Frahm
citation:
ama: 'Hernández Rodriguez T, Frahm B. Digitale Seed Train Zwillinge und statistische
Methoden. In: Herwig C, Pörtner R, Möller J, eds. Digitale Zwillinge - Werkzeuge
und Konzepte für intelligente Bioproduktion. Advances in Biochemical Engineering/Biotechnology.
Springer ; 2025:107-145. doi:https://doi.org/10.1007/978-3-031-75698-6_5'
apa: Hernández Rodriguez, T., & Frahm, B. (2025). Digitale Seed Train Zwillinge
und statistische Methoden. In C. Herwig, R. Pörtner, & J. Möller (Eds.), Digitale
Zwillinge - Werkzeuge und Konzepte für intelligente Bioproduktion (pp. 107–145).
Springer . https://doi.org/10.1007/978-3-031-75698-6_5
bjps: 'Hernández Rodriguez T and Frahm B (2025) Digitale Seed Train Zwillinge
und statistische Methoden. In Herwig C, Pörtner R and Möller J (eds), Digitale
Zwillinge - Werkzeuge und Konzepte für intelligente Bioproduktion. Berlin,
Heidelberg: Springer , pp. 107–145.'
chicago: 'Hernández Rodriguez, Tanja, and Björn Frahm. “Digitale Seed Train Zwillinge
und statistische Methoden.” In Digitale Zwillinge - Werkzeuge und Konzepte
für intelligente Bioproduktion, edited by Christoph Herwig, Ralf Pörtner,
and Johannes Möller, 107–45. Advances in Biochemical Engineering/Biotechnology.
Berlin, Heidelberg: Springer , 2025. https://doi.org/10.1007/978-3-031-75698-6_5.'
chicago-de: 'Hernández Rodriguez, Tanja und Björn Frahm. 2025. Digitale Seed Train
Zwillinge und statistische Methoden. In: Digitale Zwillinge - Werkzeuge und
Konzepte für intelligente Bioproduktion, hg. von Christoph Herwig, Ralf Pörtner,
und Johannes Möller, 107–145. Advances in Biochemical Engineering/Biotechnology.
Berlin, Heidelberg: Springer . doi:https://doi.org/10.1007/978-3-031-75698-6_5,
.'
din1505-2-1: 'Hernández Rodriguez, Tanja
; Frahm, Björn: Digitale Seed Train
Zwillinge und statistische Methoden. In: Herwig,
C. ; Pörtner, R. ; Möller, J. (Hrsg.): Digitale Zwillinge
- Werkzeuge und Konzepte für intelligente Bioproduktion, Advances in Biochemical
Engineering/Biotechnology. Berlin, Heidelberg : Springer , 2025, S. 107–145'
havard: 'T. Hernández Rodriguez, B. Frahm, Digitale Seed Train Zwillinge und statistische
Methoden, in: C. Herwig, R. Pörtner, J. Möller (Eds.), Digitale Zwillinge - Werkzeuge
und Konzepte für intelligente Bioproduktion, Springer , Berlin, Heidelberg, 2025:
pp. 107–145.'
ieee: 'T. Hernández Rodriguez and B. Frahm, “Digitale Seed Train Zwillinge und statistische
Methoden,” in Digitale Zwillinge - Werkzeuge und Konzepte für intelligente
Bioproduktion, C. Herwig, R. Pörtner, and J. Möller, Eds. Berlin, Heidelberg:
Springer , 2025, pp. 107–145. doi: https://doi.org/10.1007/978-3-031-75698-6_5.'
mla: Hernández Rodriguez, Tanja, and Björn Frahm. “Digitale Seed Train Zwillinge
und statistische Methoden.” Digitale Zwillinge - Werkzeuge und Konzepte für
intelligente Bioproduktion, edited by Christoph Herwig et al., Springer ,
2025, pp. 107–45, https://doi.org/10.1007/978-3-031-75698-6_5.
short: 'T. Hernández Rodriguez, B. Frahm, in: C. Herwig, R. Pörtner, J. Möller (Eds.),
Digitale Zwillinge - Werkzeuge und Konzepte für intelligente Bioproduktion, Springer
, Berlin, Heidelberg, 2025, pp. 107–145.'
ufg: 'Hernández Rodriguez, Tanja/Frahm, Björn: Digitale Seed Train Zwillinge
und statistische Methoden, in: Herwig, Christoph/Pörtner, Ralf/Möller, Johannes
(Hgg.): Digitale Zwillinge - Werkzeuge und Konzepte für intelligente Bioproduktion,
Berlin, Heidelberg 2025 (Advances in Biochemical Engineering/Biotechnology),
S. 107–145.'
van: 'Hernández Rodriguez T, Frahm B. Digitale Seed Train Zwillinge und statistische
Methoden. In: Herwig C, Pörtner R, Möller J, editors. Digitale Zwillinge - Werkzeuge
und Konzepte für intelligente Bioproduktion. Berlin, Heidelberg: Springer ; 2025.
p. 107–45. (Advances in Biochemical Engineering/Biotechnology).'
date_created: 2026-03-27T17:45:02Z
date_updated: 2026-04-08T12:46:45Z
department:
- _id: DEP4000
doi: https://doi.org/10.1007/978-3-031-75698-6_5
editor:
- first_name: Christoph
full_name: Herwig, Christoph
last_name: Herwig
- first_name: Ralf
full_name: Pörtner, Ralf
last_name: Pörtner
- first_name: Johannes
full_name: Möller, Johannes
last_name: Möller
keyword:
- Bayes
- Digitaler Zwilling
- Parameterabschätzung
- Seed-Train
- Unsicherheit
language:
- iso: ger
page: 107-145
place: Berlin, Heidelberg
publication: Digitale Zwillinge - Werkzeuge und Konzepte für intelligente Bioproduktion
publication_identifier:
eisbn:
- 978-3-031-75698-6
isbn:
- 978-3-031-75697-9
publication_status: published
publisher: 'Springer '
quality_controlled: '1'
series_title: Advances in Biochemical Engineering/Biotechnology
status: public
title: Digitale Seed Train Zwillinge und statistische Methoden
type: book_chapter
user_id: '83781'
year: '2025'
...
---
_id: '13020'
abstract:
- lang: eng
text: Developing AI systems for automatic train operation (ATO) requires developers
to have a deep understanding of the human tasks they are trying to replace. This
paper fills this gap and translates the regulatory requirements from the context
of German railways for the AI developer community. As a result, tasks such as
train’s path monitoring for collision prediction, signal detection, door operation,
etc. are identified. Based on this analysis, a functionally justified sensor setup
with detailed configuration requirements is presented. This setup was also evaluated
by a survey within the railway industry. The evaluated sensors include RGB/IR
cameras, LIDARs, radars and ultrasonic sensors. Calculations and estimates for
the evaluated sensors are presented graphically and included in this paper. However,
the ultimate sensor setup is still a subject of research. The results of this
paper also address the lack of training and test datasets for railway AI systems.
It is proposed to acquire research datasets that will allow the training of domain
adaptation algorithms to transform other datasets, thus increasing the number
of available datasets. The sensor setup is also recommended for such research
datasets.
article_number: '22'
author:
- first_name: Rustam
full_name: Tagiew, Rustam
last_name: Tagiew
- first_name: Dirk
full_name: Leinhos, Dirk
last_name: Leinhos
- first_name: Henrik
full_name: von der Haar, Henrik
last_name: von der Haar
- first_name: Christian
full_name: Klotz, Christian
last_name: Klotz
- first_name: Dennis
full_name: Sprute, Dennis
last_name: Sprute
- first_name: Jens
full_name: Ziehn, Jens
last_name: Ziehn
- first_name: Andreas
full_name: Schmelter, Andreas
id: '10982'
last_name: Schmelter
- first_name: Stefan
full_name: Witte, Stefan
id: '1605'
last_name: Witte
- first_name: Pavel
full_name: Klasek, Pavel
last_name: Klasek
citation:
ama: Tagiew R, Leinhos D, von der Haar H, et al. Sensor system for development of
perception systems for ATO. Discover Artificial Intelligence. 2023;3(1).
doi:10.1007/s44163-023-00066-4
apa: Tagiew, R., Leinhos, D., von der Haar, H., Klotz, C., Sprute, D., Ziehn, J.,
Schmelter, A., Witte, S., & Klasek, P. (2023). Sensor system for development
of perception systems for ATO. Discover Artificial Intelligence, 3(1),
Article 22. https://doi.org/10.1007/s44163-023-00066-4
bjps: Tagiew R et al. (2023) Sensor System for Development of Perception
Systems for ATO. Discover Artificial Intelligence 3.
chicago: Tagiew, Rustam, Dirk Leinhos, Henrik von der Haar, Christian Klotz, Dennis
Sprute, Jens Ziehn, Andreas Schmelter, Stefan Witte, and Pavel Klasek. “Sensor
System for Development of Perception Systems for ATO.” Discover Artificial
Intelligence 3, no. 1 (2023). https://doi.org/10.1007/s44163-023-00066-4.
chicago-de: Tagiew, Rustam, Dirk Leinhos, Henrik von der Haar, Christian Klotz,
Dennis Sprute, Jens Ziehn, Andreas Schmelter, Stefan Witte und Pavel Klasek. 2023.
Sensor system for development of perception systems for ATO. Discover Artificial
Intelligence 3, Nr. 1. doi:10.1007/s44163-023-00066-4,
.
din1505-2-1: 'Tagiew,
Rustam ; Leinhos, Dirk ;
von der Haar, Henrik ; Klotz,
Christian ; Sprute, Dennis
; Ziehn, Jens ; Schmelter,
Andreas ; Witte, Stefan ;
u. a.: Sensor system for development of perception systems for ATO. In:
Discover Artificial Intelligence Bd. 3. [Cham] , Springer International
Publishing (2023), Nr. 1'
havard: R. Tagiew, D. Leinhos, H. von der Haar, C. Klotz, D. Sprute, J. Ziehn, A.
Schmelter, S. Witte, P. Klasek, Sensor system for development of perception systems
for ATO, Discover Artificial Intelligence. 3 (2023).
ieee: 'R. Tagiew et al., “Sensor system for development of perception systems
for ATO,” Discover Artificial Intelligence, vol. 3, no. 1, Art. no. 22,
2023, doi: 10.1007/s44163-023-00066-4.'
mla: Tagiew, Rustam, et al. “Sensor System for Development of Perception Systems
for ATO.” Discover Artificial Intelligence, vol. 3, no. 1, 22, 2023, https://doi.org/10.1007/s44163-023-00066-4.
short: R. Tagiew, D. Leinhos, H. von der Haar, C. Klotz, D. Sprute, J. Ziehn, A.
Schmelter, S. Witte, P. Klasek, Discover Artificial Intelligence 3 (2023).
ufg: 'Tagiew, Rustam u. a.: Sensor system for development of perception systems
for ATO, in: Discover Artificial Intelligence 3 (2023), H. 1.'
van: Tagiew R, Leinhos D, von der Haar H, Klotz C, Sprute D, Ziehn J, et al. Sensor
system for development of perception systems for ATO. Discover Artificial Intelligence.
2023;3(1).
date_created: 2025-06-24T13:47:12Z
date_updated: 2025-06-24T13:51:24Z
department:
- _id: DEP5000
- _id: DEP5023
doi: 10.1007/s44163-023-00066-4
intvolume: ' 3'
issue: '1'
keyword:
- Automatic train operation
- ATO
- GoA3
- GoA4
- Perception
- AI
language:
- iso: eng
place: '[Cham] '
publication: Discover Artificial Intelligence
publication_identifier:
issn:
- 2731-0809
publication_status: published
publisher: 'Springer International Publishing '
quality_controlled: '1'
status: public
title: Sensor system for development of perception systems for ATO
type: scientific_journal_article
user_id: '83781'
volume: 3
year: '2023'
...
---
_id: '13021'
abstract:
- lang: eng
text: Natural ventilation in a building is an effective way to achieve acceptable
indoor air quality. Ventilation dilutes contaminants such as bioeffluents generated
by occupants, substances emitted from building materials, and the water vapor
generated by occupants’ activities. In a building that requires heating and cooling,
adequate ventilation is crucial to minimize energy consumption while maintaining
healthy indoor air quality. However, measuring the actual magnitude of the natural
ventilation rate, including infiltration through the building envelope and airflow
through the building openings, is not always feasible. Although international
and national standards suggested the required ventilation rates to maintain acceptable
indoor air quality in buildings, they did not offer action plans to achieve or
evaluate those design ventilation rates in buildings in use. In this study, the
occupant-generated carbon dioxide (CO2) tracer gas decay method was applied to
estimate the ventilation rates in an office room in Seoul, South Korea, from summer
to winter. Using the method, real-time ventilation rates can be calculated by
monitoring indoor and outdoor CO2 concentrations without injecting a tracer gas.
For natural ventilation in the test room, 145 mm-diameter circular openings on
the fixed glass were used. As a result, first, the indoor CO2 concentrations were
used as an indicator to evaluate how much the indoor air quality deteriorated
when all the windows were closed in an occupied office room compared to the international
standards for indoor air quality. Moreover, we found out that the estimated ventilation
rates varied depending on various environmental conditions, even with the same
openings for natural ventilation. Considering the indoor and outdoor temperature
differences and outdoor wind speeds as the main factors influencing the ventilation
rates, we analyzed how they affected the ventilation rates in the different seasons
of South Korea. When the wind speeds were calm, less than 2 m/s, the temperature
difference played as a factor that influenced the estimated ventilation rates.
On the other hand, when the temperature differences were low, less than 3 °C,
the wind speed was the primary factor. This study raises awareness about the risk
of poor indoor air quality in office rooms that could lead to health problems
or unpleasant working environments. This study presents an example of estimating
the ventilation rates in an existing building. By using the presented method,
the ventilation rate in an existing building can be simply estimated while using
the building as usual, and appropriate ventilation strategies for the building
can be determined to maintain the desired indoor air quality.
article_number: '9892'
author:
- first_name: Hyeonji
full_name: Seol, Hyeonji
id: '77436'
last_name: Seol
- first_name: Daniel
full_name: Arztmann, Daniel
id: '58805'
last_name: Arztmann
- first_name: Naree
full_name: Kim, Naree
last_name: Kim
- first_name: Alvaro
full_name: Balderrama, Alvaro
id: '79418'
last_name: Balderrama
citation:
ama: Seol H, Arztmann D, Kim N, Balderrama A. Estimation of Natural Ventilation
Rates in an Office Room with 145 mm-Diameter Circular Openings Using the Occupant-Generated
Tracer-Gas Method. Sustainability. 2023;15(13). doi:10.3390/su15139892
apa: Seol, H., Arztmann, D., Kim, N., & Balderrama, A. (2023). Estimation of
Natural Ventilation Rates in an Office Room with 145 mm-Diameter Circular Openings
Using the Occupant-Generated Tracer-Gas Method. Sustainability, 15(13),
Article 9892. https://doi.org/10.3390/su15139892
bjps: Seol H et al. (2023) Estimation of Natural Ventilation Rates
in an Office Room with 145 Mm-Diameter Circular Openings Using the Occupant-Generated
Tracer-Gas Method. Sustainability 15.
chicago: Seol, Hyeonji, Daniel Arztmann, Naree Kim, and Alvaro Balderrama. “Estimation
of Natural Ventilation Rates in an Office Room with 145 Mm-Diameter Circular Openings
Using the Occupant-Generated Tracer-Gas Method.” Sustainability 15, no.
13 (2023). https://doi.org/10.3390/su15139892.
chicago-de: Seol, Hyeonji, Daniel Arztmann, Naree Kim und Alvaro Balderrama. 2023.
Estimation of Natural Ventilation Rates in an Office Room with 145 mm-Diameter
Circular Openings Using the Occupant-Generated Tracer-Gas Method. Sustainability
15, Nr. 13. doi:10.3390/su15139892,
.
din1505-2-1: 'Seol, Hyeonji ; Arztmann, Daniel ; Kim,
Naree ; Balderrama, Alvaro:
Estimation of Natural Ventilation Rates in an Office Room with 145 mm-Diameter
Circular Openings Using the Occupant-Generated Tracer-Gas Method. In: Sustainability
Bd. 15. Basel, MDPI (2023), Nr. 13'
havard: H. Seol, D. Arztmann, N. Kim, A. Balderrama, Estimation of Natural Ventilation
Rates in an Office Room with 145 mm-Diameter Circular Openings Using the Occupant-Generated
Tracer-Gas Method, Sustainability. 15 (2023).
ieee: 'H. Seol, D. Arztmann, N. Kim, and A. Balderrama, “Estimation of Natural Ventilation
Rates in an Office Room with 145 mm-Diameter Circular Openings Using the Occupant-Generated
Tracer-Gas Method,” Sustainability, vol. 15, no. 13, Art. no. 9892, 2023,
doi: 10.3390/su15139892.'
mla: Seol, Hyeonji, et al. “Estimation of Natural Ventilation Rates in an Office
Room with 145 Mm-Diameter Circular Openings Using the Occupant-Generated Tracer-Gas
Method.” Sustainability, vol. 15, no. 13, 9892, 2023, https://doi.org/10.3390/su15139892.
short: H. Seol, D. Arztmann, N. Kim, A. Balderrama, Sustainability 15 (2023).
ufg: 'Seol, Hyeonji u. a.: Estimation of Natural Ventilation Rates in an
Office Room with 145 mm-Diameter Circular Openings Using the Occupant-Generated
Tracer-Gas Method, in: Sustainability 15 (2023), H. 13.'
van: Seol H, Arztmann D, Kim N, Balderrama A. Estimation of Natural Ventilation
Rates in an Office Room with 145 mm-Diameter Circular Openings Using the Occupant-Generated
Tracer-Gas Method. Sustainability. 2023;15(13).
date_created: 2025-06-24T13:53:29Z
date_updated: 2025-06-24T13:55:57Z
department:
- _id: DEP1600
- _id: DEP1634
doi: 10.3390/su15139892
intvolume: ' 15'
issue: '13'
keyword:
- Management
- Monitoring
- Policy and Law
- Renewable Energy
- Sustainability and the Environment
- Geography
- Planning and Development
- Building and Construction
language:
- iso: eng
place: Basel
publication: Sustainability
publication_identifier:
issn:
- 2071-1050
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Estimation of Natural Ventilation Rates in an Office Room with 145 mm-Diameter
Circular Openings Using the Occupant-Generated Tracer-Gas Method
type: scientific_journal_article
user_id: '83781'
volume: 15
year: '2023'
...
---
_id: '11377'
abstract:
- lang: eng
text: consuming and often performed rather empirically. Efficient optimization
of multiple objectives such as process time, viable cell density, number of operating
steps & cultivation scales, required medium, amount of product as well as
product quality depicts a promising approach. This contribution presents a workflow
which couples uncertainty-based upstream simulation and Bayes optimization using
Gaussian processes. Its application is demonstrated in a simulation case study
for a relevant industrial task in process development, the design of a robust
cell culture expansion process (seed train), meaning that despite uncertainties
and variabilities concerning cell growth, low variations of viable cell density
during the seed train are obtained. Compared to a non-optimized reference seed
train, the optimized process showed much lower deviation rates regarding viable
cell densities (<10% instead of 41.7%) using five or four shake flask scales
and seed train duration could be reduced by 56 h from 576 h to 520 h. Overall,
it is shown that applying Bayes optimization allows for optimization of a multi-objective
optimization function with several optimizable input variables and under a considerable
amount of constraints with a low computational effort. This approach provides
the potential to be used in the form of a decision tool, e.g., for the choice
of an optimal and robust seed train design or for further optimization tasks within
process development.
article_number: '883'
author:
- first_name: Tanja
full_name: Hernández Rodriguez, Tanja
id: '52466'
last_name: Hernández Rodriguez
- first_name: Anton
full_name: Sekulic, Anton
last_name: Sekulic
- first_name: Markus
full_name: Lange-Hegermann, Markus
id: '71761'
last_name: Lange-Hegermann
- first_name: Björn
full_name: Frahm, Björn
id: '45666'
last_name: Frahm
citation:
ama: Hernández Rodriguez T, Sekulic A, Lange-Hegermann M, Frahm B. Designing Robust
Biotechnological Processes Regarding Variabilities Using Multi-Objective Optimization
Applied to a Biopharmaceutical Seed Train Design. Processes. 2022;10(5).
doi:10.3390/pr10050883
apa: Hernández Rodriguez, T., Sekulic, A., Lange-Hegermann, M., & Frahm, B.
(2022). Designing Robust Biotechnological Processes Regarding Variabilities Using
Multi-Objective Optimization Applied to a Biopharmaceutical Seed Train Design.
Processes, 10(5), Article 883. https://doi.org/10.3390/pr10050883
bjps: Hernández Rodriguez T et al. (2022) Designing Robust Biotechnological
Processes Regarding Variabilities Using Multi-Objective Optimization Applied to
a Biopharmaceutical Seed Train Design. Processes 10.
chicago: Hernández Rodriguez, Tanja, Anton Sekulic, Markus Lange-Hegermann, and
Björn Frahm. “Designing Robust Biotechnological Processes Regarding Variabilities
Using Multi-Objective Optimization Applied to a Biopharmaceutical Seed Train Design.”
Processes 10, no. 5 (2022). https://doi.org/10.3390/pr10050883.
chicago-de: Hernández Rodriguez, Tanja, Anton Sekulic, Markus Lange-Hegermann und
Björn Frahm. 2022. Designing Robust Biotechnological Processes Regarding Variabilities
Using Multi-Objective Optimization Applied to a Biopharmaceutical Seed Train Design.
Processes 10, Nr. 5. doi:10.3390/pr10050883,
.
din1505-2-1: 'Hernández Rodriguez, Tanja
; Sekulic, Anton ; Lange-Hegermann,
Markus ; Frahm, Björn: Designing
Robust Biotechnological Processes Regarding Variabilities Using Multi-Objective
Optimization Applied to a Biopharmaceutical Seed Train Design. In: Processes
Bd. 10. Basel, MDPI AG (2022), Nr. 5'
havard: T. Hernández Rodriguez, A. Sekulic, M. Lange-Hegermann, B. Frahm, Designing
Robust Biotechnological Processes Regarding Variabilities Using Multi-Objective
Optimization Applied to a Biopharmaceutical Seed Train Design, Processes. 10 (2022).
ieee: 'T. Hernández Rodriguez, A. Sekulic, M. Lange-Hegermann, and B. Frahm, “Designing
Robust Biotechnological Processes Regarding Variabilities Using Multi-Objective
Optimization Applied to a Biopharmaceutical Seed Train Design,” Processes,
vol. 10, no. 5, Art. no. 883, 2022, doi: 10.3390/pr10050883.'
mla: Hernández Rodriguez, Tanja, et al. “Designing Robust Biotechnological Processes
Regarding Variabilities Using Multi-Objective Optimization Applied to a Biopharmaceutical
Seed Train Design.” Processes, vol. 10, no. 5, 883, 2022, https://doi.org/10.3390/pr10050883.
short: T. Hernández Rodriguez, A. Sekulic, M. Lange-Hegermann, B. Frahm, Processes
10 (2022).
ufg: 'Hernández Rodriguez, Tanja u. a.: Designing Robust Biotechnological
Processes Regarding Variabilities Using Multi-Objective Optimization Applied to
a Biopharmaceutical Seed Train Design, in: Processes 10 (2022), H. 5.'
van: Hernández Rodriguez T, Sekulic A, Lange-Hegermann M, Frahm B. Designing Robust
Biotechnological Processes Regarding Variabilities Using Multi-Objective Optimization
Applied to a Biopharmaceutical Seed Train Design. Processes. 2022;10(5).
date_created: 2024-04-25T13:35:04Z
date_updated: 2024-05-21T09:30:15Z
department:
- _id: DEP4000
doi: 10.3390/pr10050883
intvolume: ' 10'
issue: '5'
keyword:
- Gaussian processes
- Bayes optimization
- Pareto optimization
- multi-objective
- cell culture
- seed train
language:
- iso: eng
place: Basel
publication: Processes
publication_identifier:
eissn:
- 2227-9717
publication_status: published
publisher: MDPI AG
status: public
title: Designing Robust Biotechnological Processes Regarding Variabilities Using Multi-Objective
Optimization Applied to a Biopharmaceutical Seed Train Design
type: scientific_journal_article
user_id: '83781'
volume: 10
year: '2022'
...
---
_id: '7578'
abstract:
- lang: eng
text: "In recent years considerable research efforts have been made to provide evidence
for a nexus be-tween game design elements in non-game contexts. Our research presents
a new approach to bridge game design elements and educational theory: defining
a set of motivational “patterns” used for peda-gogical purposes in university
teaching scenarios. To this end, we will build upon preliminary empirical results
from a research project called EMPAMOS®. It derived a set of motivational elements
frequently used in social game designs. Our hypothesis is that these elements
resemble on a structural level and are directly transferable to motivational factors
in online education contexts. \r\nFocused on cooperative teaching and learning,
we develop a curriculum to enable educators to im-plement motivational molecules
from game design in their learning settings. The paper presents basic premises
and a preliminary structure of the curriculum. By examining educational settings
in terms of a “broken game”, we provide a new perspective on the prerequisites
for learning at the university level."
author:
- first_name: Thomas
full_name: Bröker, Thomas
last_name: Bröker
- first_name: Nina
full_name: Schmulius, Nina
id: '81110'
last_name: Schmulius
- first_name: Tobias
full_name: Schmohl, Tobias
id: '71782'
last_name: Schmohl
orcid: https://orcid.org/0000-0002-7043-5582
- first_name: Fabian
full_name: Dulisch, Fabian
last_name: Dulisch
- first_name: Sabrina
full_name: Marquardt, Sabrina
id: '81111'
last_name: Marquardt
- first_name: Max
full_name: Höllen, Max
last_name: Höllen
- first_name: Thomas
full_name: Voit, Thomas
last_name: Voit
- first_name: Benjamin
full_name: Zinger, Benjamin
last_name: Zinger
citation:
ama: Bröker T, Schmulius N, Schmohl T, et al. What Can Educators Learn from Social
Game Design in University Online Teaching? Vol 11. Libreriauniversitaria.it;
2022:22-26.
apa: Bröker, T., Schmulius, N., Schmohl, T., Dulisch, F., Marquardt, S., Höllen,
M., Voit, T., & Zinger, B. (2022). What Can Educators Learn from Social Game
Design in University Online Teaching? In New Perspectives in Science Education
(Vol. 11, pp. 22–26). Libreriauniversitaria.it.
bjps: 'Bröker T et al. (2022) What Can Educators Learn from Social
Game Design in University Online Teaching? Bologna: Libreriauniversitaria.it.'
chicago: 'Bröker, Thomas, Nina Schmulius, Tobias Schmohl, Fabian Dulisch, Sabrina
Marquardt, Max Höllen, Thomas Voit, and Benjamin Zinger. What Can Educators
Learn from Social Game Design in University Online Teaching? New Perspectives
in Science Education. Vol. 11. New Perspectives in Science Education. Bologna:
Libreriauniversitaria.it, 2022.'
chicago-de: 'Bröker, Thomas, Nina Schmulius, Tobias Schmohl, Fabian Dulisch, Sabrina
Marquardt, Max Höllen, Thomas Voit und Benjamin Zinger. 2022. What Can Educators
Learn from Social Game Design in University Online Teaching? New Perspectives
in Science Education. Bd. 11. New Perspectives in Science Education. Bologna:
Libreriauniversitaria.it.'
din1505-2-1: 'Bröker, Thomas ; Schmulius, Nina ; Schmohl,
Tobias ; Dulisch, Fabian
; Marquardt, Sabrina ; Höllen,
Max ; Voit, Thomas ; Zinger, Benjamin: What Can Educators
Learn from Social Game Design in University Online Teaching?, New Perspectives
in Science Education. Bd. 11. Bologna : Libreriauniversitaria.it, 2022'
havard: T. Bröker, N. Schmulius, T. Schmohl, F. Dulisch, S. Marquardt, M. Höllen,
T. Voit, B. Zinger, What Can Educators Learn from Social Game Design in University
Online Teaching?, Libreriauniversitaria.it, Bologna, 2022.
ieee: 'T. Bröker et al., What Can Educators Learn from Social Game Design
in University Online Teaching?, vol. 11. Bologna: Libreriauniversitaria.it,
2022, pp. 22–26.'
mla: Bröker, Thomas, et al. “What Can Educators Learn from Social Game Design in
University Online Teaching?” New Perspectives in Science Education, vol.
11, Libreriauniversitaria.it, 2022, pp. 22–26.
short: T. Bröker, N. Schmulius, T. Schmohl, F. Dulisch, S. Marquardt, M. Höllen,
T. Voit, B. Zinger, What Can Educators Learn from Social Game Design in University
Online Teaching?, Libreriauniversitaria.it, Bologna, 2022.
ufg: 'Bröker, Thomas u. a.: What Can Educators Learn from Social Game Design
in University Online Teaching?, Bd. 11, Bologna 2022 (New Perspectives in Science
Education).'
van: 'Bröker T, Schmulius N, Schmohl T, Dulisch F, Marquardt S, Höllen M, et al.
What Can Educators Learn from Social Game Design in University Online Teaching?
New Perspectives in Science Education. Bologna: Libreriauniversitaria.it; 2022.
(New Perspectives in Science Education; vol. 11).'
conference:
end_date: 2022-03-18
location: Florenz
name: 11th International Conference New Perspectives in Science Education
start_date: 2022-03-17
date_created: 2022-04-14T10:58:17Z
date_updated: 2024-08-12T08:07:32Z
department:
- _id: DEP2000
- _id: DEP1200
intvolume: ' 11'
keyword:
- cooperative learning
- gamification
- motivation
- train-the-trainer
- curriculum
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://conference.pixel-online.net/NPSE/files/npse/ed0011/FP/6790-CDEV5427-FP-NPSE11.pdf
oa: '1'
page: 22-26
place: Bologna
publication: New Perspectives in Science Education
publication_status: published
publisher: Libreriauniversitaria.it
quality_controlled: '1'
series_title: New Perspectives in Science Education
status: public
title: What Can Educators Learn from Social Game Design in University Online Teaching?
type: conference_editor_article
user_id: '83781'
volume: 11
year: '2022'
...
---
_id: '10193'
abstract:
- lang: eng
text: Development and optimization of biopharmaceutical production processes with
cell cultures is cost- and time-consuming and often performed rather empirically.
Efficient optimization of multiple objectives such as process time, viable cell
density, number of operating steps & cultivation scales, required medium, amount
of product as well as product quality depicts a promising approach. This contribution
presents a workflow which couples uncertainty-based upstream simulation and Bayes
optimization using Gaussian processes. Its application is demonstrated in a simulation
case study for a relevant industrial task in process development, the design of
a robust cell culture expansion process (seed train), meaning that despite uncertainties
and variabilities concerning cell growth, low variations of viable cell density
during the seed train are obtained. Compared to a non-optimized reference seed
train, the optimized process showed much lower deviation rates regarding viable
cell densities (<10% instead of 41.7%) using five or four shake flask scales and
seed train duration could be reduced by 56 h from 576 h to 520 h. Overall, it
is shown that applying Bayes optimization allows for optimization of a multi-objective
optimization function with several optimizable input variables and under a considerable
amount of constraints with a low computational effort. This approach provides
the potential to be used in the form of a decision tool, e.g., for the choice
of an optimal and robust seed train design or for further optimization tasks within
process development.
author:
- first_name: Tanja
full_name: Hernández Rodriguez, Tanja
id: '52466'
last_name: Hernández Rodriguez
- first_name: Anton
full_name: Sekulic, Anton
last_name: Sekulic
- first_name: Markus
full_name: Lange-Hegermann, Markus
id: '71761'
last_name: Lange-Hegermann
- first_name: Björn
full_name: Frahm, Björn
id: '45666'
last_name: Frahm
citation:
ama: 'Hernández Rodriguez T, Sekulic A, Lange-Hegermann M, Frahm B. Designing robust
biotechnological processes regarding variabilities using multi-objective optimization
applied to a biopharmaceutical seed train design. In: Pörtner R, Möller J, eds.
Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing.
Vol special issue. Processes : open access journal. MDPI; 2022:21-48. doi:https://doi.org/10.3390/pr10050883'
apa: 'Hernández Rodriguez, T., Sekulic, A., Lange-Hegermann, M., & Frahm, B.
(2022). Designing robust biotechnological processes regarding variabilities using
multi-objective optimization applied to a biopharmaceutical seed train design.
In R. Pörtner & J. Möller (Eds.), Bioprocess Systems Engineering Applications
in Pharmaceutical Manufacturing: Vol. special issue (pp. 21–48). MDPI. https://doi.org/10.3390/pr10050883'
bjps: 'Hernández Rodriguez T et al. (2022) Designing Robust Biotechnological
Processes Regarding Variabilities Using Multi-Objective Optimization Applied to
a Biopharmaceutical Seed Train Design. In Pörtner R and Möller J (eds), Bioprocess
Systems Engineering Applications in Pharmaceutical Manufacturing, vol. special
issue. Basel: MDPI, pp. 21–48.'
chicago: 'Hernández Rodriguez, Tanja, Anton Sekulic, Markus Lange-Hegermann, and
Björn Frahm. “Designing Robust Biotechnological Processes Regarding Variabilities
Using Multi-Objective Optimization Applied to a Biopharmaceutical Seed Train Design.”
In Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing,
edited by Ralf Pörtner and Johannes Möller, special issue:21–48. Processes : Open
Access Journal. Basel: MDPI, 2022. https://doi.org/10.3390/pr10050883.'
chicago-de: 'Hernández Rodriguez, Tanja, Anton Sekulic, Markus Lange-Hegermann und
Björn Frahm. 2022. Designing robust biotechnological processes regarding variabilities
using multi-objective optimization applied to a biopharmaceutical seed train design.
In: Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing,
hg. von Ralf Pörtner und Johannes Möller, special issue:21–48. Processes : open
access journal. Basel: MDPI. doi:https://doi.org/10.3390/pr10050883,
.'
din1505-2-1: 'Hernández Rodriguez, Tanja
; Sekulic, Anton ; Lange-Hegermann,
Markus ; Frahm, Björn: Designing
robust biotechnological processes regarding variabilities using multi-objective
optimization applied to a biopharmaceutical seed train design. In: Pörtner,
R. ; Möller, J. (Hrsg.):
Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing,
Processes : open access journal. Bd. special issue. Basel : MDPI, 2022,
S. 21–48'
havard: 'T. Hernández Rodriguez, A. Sekulic, M. Lange-Hegermann, B. Frahm, Designing
robust biotechnological processes regarding variabilities using multi-objective
optimization applied to a biopharmaceutical seed train design, in: R. Pörtner,
J. Möller (Eds.), Bioprocess Systems Engineering Applications in Pharmaceutical
Manufacturing, MDPI, Basel, 2022: pp. 21–48.'
ieee: 'T. Hernández Rodriguez, A. Sekulic, M. Lange-Hegermann, and B. Frahm, “Designing
robust biotechnological processes regarding variabilities using multi-objective
optimization applied to a biopharmaceutical seed train design,” in Bioprocess
Systems Engineering Applications in Pharmaceutical Manufacturing, vol. special
issue, R. Pörtner and J. Möller, Eds. Basel: MDPI, 2022, pp. 21–48. doi: https://doi.org/10.3390/pr10050883.'
mla: Hernández Rodriguez, Tanja, et al. “Designing Robust Biotechnological Processes
Regarding Variabilities Using Multi-Objective Optimization Applied to a Biopharmaceutical
Seed Train Design.” Bioprocess Systems Engineering Applications in Pharmaceutical
Manufacturing, edited by Ralf Pörtner and Johannes Möller, vol. special issue,
MDPI, 2022, pp. 21–48, https://doi.org/10.3390/pr10050883.
short: 'T. Hernández Rodriguez, A. Sekulic, M. Lange-Hegermann, B. Frahm, in: R.
Pörtner, J. Möller (Eds.), Bioprocess Systems Engineering Applications in Pharmaceutical
Manufacturing, MDPI, Basel, 2022, pp. 21–48.'
ufg: 'Hernández Rodriguez, Tanja u. a.: Designing robust biotechnological
processes regarding variabilities using multi-objective optimization applied to
a biopharmaceutical seed train design, in: Pörtner, Ralf/Möller, Johannes (Hgg.):
Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing, Band
special issue, Basel 2022 (Processes : open access journal), S. 21–48.'
van: 'Hernández Rodriguez T, Sekulic A, Lange-Hegermann M, Frahm B. Designing robust
biotechnological processes regarding variabilities using multi-objective optimization
applied to a biopharmaceutical seed train design. In: Pörtner R, Möller J, editors.
Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing. Basel:
MDPI; 2022. p. 21–48. (Processes : open access journal; vol. special issue).'
date_created: 2023-08-08T12:58:36Z
date_updated: 2023-08-16T09:24:16Z
department:
- _id: DEP4000
doi: https://doi.org/10.3390/pr10050883
editor:
- first_name: Ralf
full_name: Pörtner, Ralf
last_name: Pörtner
- first_name: Johannes
full_name: Möller, Johannes
last_name: Möller
keyword:
- Gaussian processes
- Bayes optimization
- Pareto optimization
- multi-objective
- cell culture
- seed train
language:
- iso: eng
page: 21-48
place: Basel
publication: Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing
publication_identifier:
eisbn:
- 978-3-0365-5209-5
eissn:
- 2227-9717
isbn:
- 978-3-0365-5210-1
publication_status: published
publisher: MDPI
quality_controlled: '1'
series_title: 'Processes : open access journal'
status: public
title: Designing robust biotechnological processes regarding variabilities using multi-objective
optimization applied to a biopharmaceutical seed train design
type: book_chapter
user_id: '83781'
volume: special issue
year: '2022'
...
---
_id: '10590'
abstract:
- lang: ger
text: Das Kriechende Netzblatt, Goodyera repens, das von den Vorständen der Arbeitskreise
Heimische Orchideen Deutschlands (AHOs) zur Orchidee des Jahres 2021 gewählt wurde,
wird vorgestellt. Namensgebung, morphologische Merkmale und Lebenszyklus sowie
Ökologie und Lebensräume werden charakterisiert. Das Areal der Art wird beschrieben
und die Verbreitung für Deutschland kartographisch dargestellt. Gefährdungsursachen
werden erörtert und Schutzmaßnahmen aufgezeigt. Abschließend werden signifikante
Konsequenzen des Klimawandels für die Art diskutiert und in Beispielen dargelegt.
author:
- first_name: Marco
full_name: Klüber, Marco
last_name: Klüber
- first_name: Mathias
full_name: Lohr, Mathias
id: '22021'
last_name: Lohr
citation:
ama: 'Klüber M, Lohr M. Das Kriechende Netzblatt Goodyera repens (L.) R.Br. – Orchidee
des Jahres 2021 . Journal europäischer Orchideen : Mitteilungsblatt des AHO
Baden-Württemberg . 2022;54(1-2):3-27.'
apa: 'Klüber, M., & Lohr, M. (2022). Das Kriechende Netzblatt Goodyera repens
(L.) R.Br. – Orchidee des Jahres 2021 . Journal europäischer Orchideen : Mitteilungsblatt
des AHO Baden-Württemberg , 54(1–2), 3–27.'
bjps: 'Klüber M and Lohr M (2022) Das Kriechende Netzblatt Goodyera repens
(L.) R.Br. – Orchidee des Jahres 2021 . Journal europäischer Orchideen : Mitteilungsblatt
des AHO Baden-Württemberg 54, 3–27.'
chicago: 'Klüber, Marco, and Mathias Lohr. “Das Kriechende Netzblatt Goodyera repens
(L.) R.Br. – Orchidee des Jahres 2021 .” Journal europäischer Orchideen :
Mitteilungsblatt des AHO Baden-Württemberg 54, no. 1–2 (2022): 3–27.'
chicago-de: 'Klüber, Marco und Mathias Lohr. 2022. Das Kriechende Netzblatt Goodyera
repens (L.) R.Br. – Orchidee des Jahres 2021 . Journal europäischer Orchideen :
Mitteilungsblatt des AHO Baden-Württemberg 54, Nr. 1–2: 3–27.'
din1505-2-1: 'Klüber, Marco ; Lohr, Mathias: Das Kriechende Netzblatt
Goodyera repens (L.) R.Br. – Orchidee des Jahres 2021 . In: Journal europäischer
Orchideen : Mitteilungsblatt des AHO Baden-Württemberg Bd. 54. Stuttgart,
AHO (2022), Nr. 1–2, S. 3–27'
havard: 'M. Klüber, M. Lohr, Das Kriechende Netzblatt Goodyera repens (L.) R.Br.
– Orchidee des Jahres 2021 , Journal europäischer Orchideen : Mitteilungsblatt
des AHO Baden-Württemberg . 54 (2022) 3–27.'
ieee: 'M. Klüber and M. Lohr, “Das Kriechende Netzblatt Goodyera repens (L.) R.Br.
– Orchidee des Jahres 2021 ,” Journal europäischer Orchideen : Mitteilungsblatt
des AHO Baden-Württemberg , vol. 54, no. 1–2, pp. 3–27, 2022.'
mla: 'Klüber, Marco, and Mathias Lohr. “Das Kriechende Netzblatt Goodyera repens
(L.) R.Br. – Orchidee des Jahres 2021 .” Journal europäischer Orchideen :
Mitteilungsblatt des AHO Baden-Württemberg , vol. 54, no. 1–2, 2022, pp. 3–27.'
short: 'M. Klüber, M. Lohr, Journal europäischer Orchideen : Mitteilungsblatt des
AHO Baden-Württemberg 54 (2022) 3–27.'
ufg: 'Klüber, Marco/Lohr, Mathias: Das Kriechende Netzblatt Goodyera repens
(L.) R.Br. – Orchidee des Jahres 2021 , in: Journal europäischer Orchideen :
Mitteilungsblatt des AHO Baden-Württemberg 54 (2022), H. 1–2, S. 3–27.'
van: 'Klüber M, Lohr M. Das Kriechende Netzblatt Goodyera repens (L.) R.Br. – Orchidee
des Jahres 2021 . Journal europäischer Orchideen : Mitteilungsblatt des AHO Baden-Württemberg
. 2022;54(1–2):3–27.'
date_created: 2023-10-25T16:32:42Z
date_updated: 2024-04-02T12:38:21Z
ddc:
- '580'
department:
- _id: DEP9012
- _id: DEP1310
has_accepted_license: '1'
intvolume: ' 54'
issue: 1-2
keyword:
- Orchidaceae
- Goodyera repens
- Orchid of the Year 2021
- characteristics
- distribution
- life history
- monitoring
- threats
- conservation status
- protection
- Germany.
language:
- iso: ger
main_file_link:
- open_access: '1'
url: https://www.researchgate.net/publication/377571933_Das_Kriechende_Netzblatt_Goodyera_repens_L_R_Brown_-_Orchidee_des_Jahres_2021
oa: '1'
page: 3-27
place: Stuttgart
publication: 'Journal europäischer Orchideen : Mitteilungsblatt des AHO Baden-Württemberg '
publication_identifier:
issn:
- 0945-7909
publication_status: published
publisher: AHO
status: public
title: 'Das Kriechende Netzblatt Goodyera repens (L.) R.Br. – Orchidee des Jahres
2021 '
type: industry_journal_article
user_id: '83781'
volume: 54
year: '2022'
...
---
_id: '11376'
abstract:
- lang: eng
text: concentration is an important objective. The phenotype of the cells
in a reactor plays an important role. Are clonal cell populations showing high
cell-specific growth rates more favorable than cell lines with higher cell-specific
productivities or vice versa? Five clonal Chinese hamster ovary cell populations
were analyzed based on the data of a 3-month-stability study. We adapted a mechanistic
cell culture model to the experimental data of one such clonally derived cell
population. Uncertainties and prior knowledge concerning model parameters were
considered using Bayesian parameter estimations. This model was used then to define
an inoculum train protocol. Based on this, we subsequently simulated the impacts
of differences in growth rates (±10%) and production rates (±10% and ±50%) on
the overall cultivation time, including making the inoculum train cultures; the
final production phase, the volumetric titer in that bioreactor and the ratio
of both, defined as overall process productivity. We showed thus unequivocally
that growth rates have a higher impact (up to three times) on overall process
productivity and for product output per year, whereas cells with higher productivity
can potentially generate higher product concentrations in the production vessel.
article_number: '964'
author:
- first_name: Tanja
full_name: Hernández Rodriguez, Tanja
id: '52466'
last_name: Hernández Rodriguez
- first_name: Sophie
full_name: Morerod, Sophie
last_name: Morerod
- first_name: Ralf
full_name: Pörtner, Ralf
last_name: Pörtner
- first_name: Florian M.
full_name: Wurm, Florian M.
last_name: Wurm
- first_name: Björn
full_name: Frahm, Björn
id: '45666'
last_name: Frahm
citation:
ama: Hernández Rodriguez T, Morerod S, Pörtner R, Wurm FM, Frahm B. Considerations
of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A
Simulation Study. Processes. 2021;9(6). doi:10.3390/pr9060964
apa: Hernández Rodriguez, T., Morerod, S., Pörtner, R., Wurm, F. M., & Frahm,
B. (2021). Considerations of the Impacts of Cell-Specific Growth and Production
Rate on Clone Selection—A Simulation Study. Processes, 9(6), Article
964. https://doi.org/10.3390/pr9060964
bjps: Hernández Rodriguez T et al. (2021) Considerations of the Impacts
of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study.
Processes 9.
chicago: Hernández Rodriguez, Tanja, Sophie Morerod, Ralf Pörtner, Florian M. Wurm,
and Björn Frahm. “Considerations of the Impacts of Cell-Specific Growth and Production
Rate on Clone Selection—A Simulation Study.” Processes 9, no. 6 (2021).
https://doi.org/10.3390/pr9060964.
chicago-de: Hernández Rodriguez, Tanja, Sophie Morerod, Ralf Pörtner, Florian M.
Wurm und Björn Frahm. 2021. Considerations of the Impacts of Cell-Specific Growth
and Production Rate on Clone Selection—A Simulation Study. Processes 9,
Nr. 6. doi:10.3390/pr9060964,
.
din1505-2-1: 'Hernández Rodriguez, Tanja
; Morerod, Sophie ; Pörtner,
Ralf ; Wurm, Florian M. ;
Frahm, Björn: Considerations of
the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation
Study. In: Processes Bd. 9. Basel, MDPI AG (2021), Nr. 6'
havard: T. Hernández Rodriguez, S. Morerod, R. Pörtner, F.M. Wurm, B. Frahm, Considerations
of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A
Simulation Study, Processes. 9 (2021).
ieee: 'T. Hernández Rodriguez, S. Morerod, R. Pörtner, F. M. Wurm, and B. Frahm,
“Considerations of the Impacts of Cell-Specific Growth and Production Rate on
Clone Selection—A Simulation Study,” Processes, vol. 9, no. 6, Art. no.
964, 2021, doi: 10.3390/pr9060964.'
mla: Hernández Rodriguez, Tanja, et al. “Considerations of the Impacts of Cell-Specific
Growth and Production Rate on Clone Selection—A Simulation Study.” Processes,
vol. 9, no. 6, 964, 2021, https://doi.org/10.3390/pr9060964.
short: T. Hernández Rodriguez, S. Morerod, R. Pörtner, F.M. Wurm, B. Frahm, Processes
9 (2021).
ufg: 'Hernández Rodriguez, Tanja u. a.: Considerations of the Impacts of
Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study,
in: Processes 9 (2021), H. 6.'
van: Hernández Rodriguez T, Morerod S, Pörtner R, Wurm FM, Frahm B. Considerations
of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A
Simulation Study. Processes. 2021;9(6).
date_created: 2024-04-25T13:34:26Z
date_updated: 2024-05-21T09:29:02Z
department:
- _id: DEP4000
doi: 10.3390/pr9060964
intvolume: ' 9'
issue: '6'
keyword:
- clonal cell population
- phenotypic diversity
- inoculum train
- uncertainty-based
- cell culture model
- biopharmaceutical manufacturing
language:
- iso: eng
place: Basel
publication: Processes
publication_identifier:
eissn:
- 2227-9717
publication_status: published
publisher: MDPI AG
status: public
title: Considerations of the Impacts of Cell-Specific Growth and Production Rate on
Clone Selection—A Simulation Study
type: scientific_journal_article
user_id: '83781'
volume: 9
year: '2021'
...
---
_id: '7518'
abstract:
- lang: eng
text: The sharing economy provides short-term access to products without the risks
associated with ownership. While extant research primarily examined it in the
context of affluent consumer segments, the sharing economy may offer opportunities
for consumers at the base of the economic pyramid, where ownership risks prevent
access to products that could create societal benefits. Drawing from risk perception
theory, we examine how access-based services, as an alternative to ownership,
can mitigate perceived risk dimensions. An experimental study reveals that, in
contrast to consumers with higher income, low-income consumers perceive access-based
services to entail less financial risk, resulting in a greater inclination to
access a good than to own it. In a second study, we explore these differences
by comparing access with a risk mitigation strategy. We find that at the base
of the pyramid, access is perceived to entail less financial risk than both ownership
and ownership with a warranty. The results indicate the importance of the sharing
economy for addressing the limited availability of resources for alleviating poverty.
Based on our findings, we derive implications for consumers and service providers
at the base of the pyramid, and discuss how the COVID-19 pandemic may be detrimental
to the identified opportunities.
article_type: original
author:
- first_name: Tobias
full_name: Schäfers, Tobias
id: '77945'
last_name: Schäfers
orcid: 0000-0002-2533-335X
- first_name: Gopalakrishnan
full_name: Narayanamurthy, Gopalakrishnan
last_name: Narayanamurthy
- first_name: Roger
full_name: Moser, Roger
last_name: Moser
- first_name: Marina
full_name: Leban, Marina
last_name: Leban
citation:
ama: 'Schäfers T, Narayanamurthy G, Moser R, Leban M. The sharing economy at the
base of the economic pyramid: How access‐based services can help overcome ownership
risks. Psychology & Marketing. 2021;38(11):2073-2088. doi:10.1002/mar.21541'
apa: 'Schäfers, T., Narayanamurthy, G., Moser, R., & Leban, M. (2021). The sharing
economy at the base of the economic pyramid: How access‐based services can help
overcome ownership risks. Psychology & Marketing, 38(11), 2073–2088.
https://doi.org/10.1002/mar.21541'
bjps: 'Schäfers T et al. (2021) The Sharing Economy at the Base of
the Economic Pyramid: How Access‐based Services Can Help Overcome Ownership Risks.
Psychology & Marketing 38, 2073–2088.'
chicago: 'Schäfers, Tobias, Gopalakrishnan Narayanamurthy, Roger Moser, and Marina
Leban. “The Sharing Economy at the Base of the Economic Pyramid: How Access‐based
Services Can Help Overcome Ownership Risks.” Psychology & Marketing
38, no. 11 (2021): 2073–88. https://doi.org/10.1002/mar.21541.'
chicago-de: 'Schäfers, Tobias, Gopalakrishnan Narayanamurthy, Roger Moser und Marina
Leban. 2021. The sharing economy at the base of the economic pyramid: How access‐based
services can help overcome ownership risks. Psychology & Marketing
38, Nr. 11: 2073–2088. doi:10.1002/mar.21541,
.'
din1505-2-1: 'Schäfers, Tobias ; Narayanamurthy, Gopalakrishnan ; Moser, Roger ; Leban,
Marina: The sharing economy at the base of the economic pyramid: How access‐based
services can help overcome ownership risks. In: Psychology & Marketing
Bd. 38. New York, NY, Wiley (2021), Nr. 11, S. 2073–2088'
havard: 'T. Schäfers, G. Narayanamurthy, R. Moser, M. Leban, The sharing economy
at the base of the economic pyramid: How access‐based services can help overcome
ownership risks, Psychology & Marketing. 38 (2021) 2073–2088.'
ieee: 'T. Schäfers, G. Narayanamurthy, R. Moser, and M. Leban, “The sharing economy
at the base of the economic pyramid: How access‐based services can help overcome
ownership risks,” Psychology & Marketing, vol. 38, no. 11, pp. 2073–2088,
2021, doi: 10.1002/mar.21541.'
mla: 'Schäfers, Tobias, et al. “The Sharing Economy at the Base of the Economic
Pyramid: How Access‐based Services Can Help Overcome Ownership Risks.” Psychology
& Marketing, vol. 38, no. 11, 2021, pp. 2073–88, https://doi.org/10.1002/mar.21541.'
short: T. Schäfers, G. Narayanamurthy, R. Moser, M. Leban, Psychology & Marketing
38 (2021) 2073–2088.
ufg: 'Schäfers, Tobias u. a.: The sharing economy at the base of the economic
pyramid: How access‐based services can help overcome ownership risks, in: Psychology
& Marketing 38 (2021), H. 11, S. 2073–2088.'
van: 'Schäfers T, Narayanamurthy G, Moser R, Leban M. The sharing economy at the
base of the economic pyramid: How access‐based services can help overcome ownership
risks. Psychology & Marketing. 2021;38(11):2073–88.'
date_created: 2022-04-13T10:45:02Z
date_updated: 2025-06-26T13:23:58Z
department:
- _id: DEP1521
doi: 10.1002/mar.21541
external_id:
isi:
- '000672550800001'
intvolume: ' 38'
isi: '1'
issue: '11'
keyword:
- access-based services
- base of the pyramid
- risk perception theory
- sharing economy
language:
- iso: eng
page: 2073-2088
place: New York, NY
publication: Psychology & Marketing
publication_identifier:
eissn:
- 1520-6793
issn:
- 0742-6046
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: 'The sharing economy at the base of the economic pyramid: How access‐based
services can help overcome ownership risks'
type: scientific_journal_article
user_id: '83781'
volume: 38
year: '2021'
...
---
_id: '7983'
abstract:
- lang: eng
text: For the manufacturing of complex biopharmaceuticals using bioreactors with
cultivated mammalian cells, high product concentration is an important objective.
The phenotype of the cells in a reactor plays an important role. Are clonal cell
populations showing high cell-specific growth rates more favorable than cell lines
with higher cell-specific productivities or vice versa? Five clonal Chinese hamster
ovary cell populations were analyzed based on the data of a 3-month-stability
study. We adapted a mechanistic cell culture model to the experimental data of
one such clonally derived cell population. Uncertainties and prior knowledge concerning
model parameters were considered using Bayesian parameter estimations. This model
was used then to define an inoculum train protocol. Based on this, we subsequently
simulated the impacts of differences in growth rates (±10%) and production rates
(±10% and ±50%) on the overall cultivation time, including making the inoculum
train cultures; the final production phase, the volumetric titer in that bioreactor
and the ratio of both, defined as overall process productivity. We showed thus
unequivocally that growth rates have a higher impact (up to three times) on overall
process productivity and for product output per year, whereas cells with higher
productivity can potentially generate higher product concentrations in the production
vessel.
author:
- first_name: Tanja
full_name: Hernández Rodriguez, Tanja
id: '52466'
last_name: Hernández Rodriguez
- first_name: Sophie
full_name: Morerod, Sophie
last_name: Morerod
- first_name: Ralf
full_name: Pörtner, Ralf
last_name: Pörtner
- first_name: Florian M.
full_name: Wurm, Florian M.
last_name: Wurm
- first_name: Björn
full_name: Frahm, Björn
id: '45666'
last_name: Frahm
citation:
ama: 'Hernández Rodriguez T, Morerod S, Pörtner R, Wurm FM, Frahm B. Considerations
of the impacts of cell-specific growth and production rate on clone selection
– a simulation study. In: Bioprocess Systems Engineering Applications in Pharmaceutical
Manufacturing. Vol special issue. Processes : open access journal . MDPI;
2021:49-74. doi:10.3390/pr9060964'
apa: 'Hernández Rodriguez, T., Morerod, S., Pörtner, R., Wurm, F. M., & Frahm,
B. (2021). Considerations of the impacts of cell-specific growth and production
rate on clone selection – a simulation study. In Bioprocess Systems Engineering
Applications in Pharmaceutical Manufacturing: Vol. special issue (pp. 49–74).
MDPI. https://doi.org/10.3390/pr9060964'
bjps: 'Hernández Rodriguez T et al. (2021) Considerations of the Impacts
of Cell-Specific Growth and Production Rate on Clone Selection – a Simulation
Study. Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing,
vol. special issue. Basel: MDPI, pp. 49–74.'
chicago: 'Hernández Rodriguez, Tanja, Sophie Morerod, Ralf Pörtner, Florian M. Wurm,
and Björn Frahm. “Considerations of the Impacts of Cell-Specific Growth and Production
Rate on Clone Selection – a Simulation Study.” In Bioprocess Systems Engineering
Applications in Pharmaceutical Manufacturing, special issue:49–74. Processes :
Open Access Journal . Basel: MDPI, 2021. https://doi.org/10.3390/pr9060964.'
chicago-de: 'Hernández Rodriguez, Tanja, Sophie Morerod, Ralf Pörtner, Florian M.
Wurm und Björn Frahm. 2021. Considerations of the impacts of cell-specific growth
and production rate on clone selection – a simulation study. In: Bioprocess
Systems Engineering Applications in Pharmaceutical Manufacturing, special
issue:49–74. Processes : open access journal . Basel: MDPI. doi:10.3390/pr9060964,
.'
din1505-2-1: 'Hernández Rodriguez, Tanja
; Morerod, Sophie ; Pörtner,
Ralf ; Wurm, Florian M. ;
Frahm, Björn: Considerations of
the impacts of cell-specific growth and production rate on clone selection – a
simulation study. In: Bioprocess Systems Engineering Applications in Pharmaceutical
Manufacturing, Processes : open access journal . Bd. special issue.
Basel : MDPI, 2021, S. 49–74'
havard: 'T. Hernández Rodriguez, S. Morerod, R. Pörtner, F.M. Wurm, B. Frahm, Considerations
of the impacts of cell-specific growth and production rate on clone selection
– a simulation study, in: Bioprocess Systems Engineering Applications in Pharmaceutical
Manufacturing, MDPI, Basel, 2021: pp. 49–74.'
ieee: 'T. Hernández Rodriguez, S. Morerod, R. Pörtner, F. M. Wurm, and B. Frahm,
“Considerations of the impacts of cell-specific growth and production rate on
clone selection – a simulation study,” in Bioprocess Systems Engineering Applications
in Pharmaceutical Manufacturing, vol. special issue, Basel: MDPI, 2021, pp.
49–74. doi: 10.3390/pr9060964.'
mla: Hernández Rodriguez, Tanja, et al. “Considerations of the Impacts of Cell-Specific
Growth and Production Rate on Clone Selection – a Simulation Study.” Bioprocess
Systems Engineering Applications in Pharmaceutical Manufacturing, vol. special
issue, MDPI, 2021, pp. 49–74, https://doi.org/10.3390/pr9060964.
short: 'T. Hernández Rodriguez, S. Morerod, R. Pörtner, F.M. Wurm, B. Frahm, in:
Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing, MDPI,
Basel, 2021, pp. 49–74.'
ufg: 'Hernández Rodriguez, Tanja u. a.: Considerations of the impacts of
cell-specific growth and production rate on clone selection – a simulation study,
in: o. Hg.: Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing,
Band special issue, Basel 2021 (Processes : open access journal ), S.
49–74.'
van: 'Hernández Rodriguez T, Morerod S, Pörtner R, Wurm FM, Frahm B. Considerations
of the impacts of cell-specific growth and production rate on clone selection
– a simulation study. In: Bioprocess Systems Engineering Applications in Pharmaceutical
Manufacturing. Basel: MDPI; 2021. p. 49–74. (Processes : open access journal ;
vol. special issue).'
date_created: 2022-05-05T12:55:52Z
date_updated: 2023-08-16T09:17:50Z
department:
- _id: DEP4021
doi: 10.3390/pr9060964
keyword:
- clonal cell population
- phenotypic diversity
- inoculum train
- uncertainty-based
- cell culture model
- biopharmaceutical manufacturing
language:
- iso: eng
page: 49-74
place: Basel
publication: Bioprocess Systems Engineering Applications in Pharmaceutical Manufacturing
publication_identifier:
eisbn:
- 978-3-0365-5209-5
isbn:
- 978-3-0365-5210-1
issn:
- '2227-9717 '
publication_status: published
publisher: MDPI
quality_controlled: '1'
series_title: 'Processes : open access journal '
status: public
title: Considerations of the impacts of cell-specific growth and production rate on
clone selection – a simulation study
type: book_chapter
user_id: '83781'
volume: special issue
year: '2021'
...
---
_id: '3349'
abstract:
- lang: eng
text: "Model-based concepts and simulation techniques in combination with digital
tools emerge as a key to explore the full potential of biopharmaceutical production
processes, which contain several challenging development and process steps. One
of these steps is the time- and cost-intensive cell proliferation process (also
called seed train) to increase cell number from cell thawing up to production
scale. Challenges like complex cell metabolism, batch-to-batch variation, variabilities
in cell behavior, and influences of changes in cultivation conditions necessitate
adequate digital solutions to provide information about the current and near future
process state to derive correct process decisions.\r\nFor this purpose digital
seed train twins have proved to be efficient, which digitally display the time-dependent
behavior of important process variables based on mathematical models, strategies,
and adaption procedures.\r\nThis chapter will outline the needs for digitalization
of seed trains, the construction of a digital seed train twin, the role of parameter
estimation, and different statistical methods within this context, which are applicable
to several problems in the field of bioprocessing. The results of a case study
are presented to illustrate a Bayesian approach for parameter estimation and prediction
of an industrial cell culture seed train for seed train digitalization."
author:
- first_name: Tanja
full_name: Hernández Rodriguez, Tanja
id: '52466'
last_name: Hernández Rodriguez
- first_name: Björn
full_name: Frahm, Björn
id: '45666'
last_name: Frahm
citation:
ama: 'Hernández Rodriguez T, Frahm B. Digital Seed Train Twins and Statistical Methods.
In: Herwig C, Pörtner R, Möller J, eds. Digital Twins Tools and Concepts for
Smart Biomanufacturing. Vol 176. Advances in Biochemical Engineering/Biotechnology.
Springer; 2021:97-131. doi:https://doi.org/10.1007/10_2020_137'
apa: Hernández Rodriguez, T., & Frahm, B. (2021). Digital Seed Train Twins and
Statistical Methods. In C. Herwig, R. Pörtner, & J. Möller (Eds.), Digital
Twins Tools and Concepts for Smart Biomanufacturing (Vol. 176, pp. 97–131).
Springer. https://doi.org/10.1007/10_2020_137
bjps: 'Hernández Rodriguez T and Frahm B (2021) Digital Seed Train Twins
and Statistical Methods. In Herwig C, Pörtner R and Möller J (eds), Digital
Twins Tools and Concepts for Smart Biomanufacturing, vol. 176. Berlin, Heidelberg:
Springer, pp. 97–131.'
chicago: 'Hernández Rodriguez, Tanja, and Björn Frahm. “Digital Seed Train Twins
and Statistical Methods.” In Digital Twins Tools and Concepts for Smart Biomanufacturing,
edited by Christoph Herwig, Ralf Pörtner, and Johannes Möller, 176:97–131.
Advances in Biochemical Engineering/Biotechnology. Berlin, Heidelberg: Springer,
2021. https://doi.org/10.1007/10_2020_137.'
chicago-de: 'Hernández Rodriguez, Tanja und Björn Frahm. 2021. Digital Seed Train
Twins and Statistical Methods. In: Digital Twins Tools and Concepts for Smart
Biomanufacturing, hg. von Christoph Herwig, Ralf Pörtner, und Johannes Möller,
176:97–131. Advances in Biochemical Engineering/Biotechnology. Berlin, Heidelberg:
Springer. doi:https://doi.org/10.1007/10_2020_137,
.'
din1505-2-1: 'Hernández Rodriguez, Tanja
; Frahm, Björn: Digital Seed Train
Twins and Statistical Methods. In: Herwig,
C. ; Pörtner, R. ; Möller, J. (Hrsg.): Digital Twins Tools
and Concepts for Smart Biomanufacturing, Advances in Biochemical Engineering/Biotechnology.
Bd. 176. Berlin, Heidelberg : Springer, 2021, S. 97–131'
havard: 'T. Hernández Rodriguez, B. Frahm, Digital Seed Train Twins and Statistical
Methods, in: C. Herwig, R. Pörtner, J. Möller (Eds.), Digital Twins Tools and
Concepts for Smart Biomanufacturing, Springer, Berlin, Heidelberg, 2021: pp. 97–131.'
ieee: 'T. Hernández Rodriguez and B. Frahm, “Digital Seed Train Twins and Statistical
Methods,” in Digital Twins Tools and Concepts for Smart Biomanufacturing,
vol. 176, C. Herwig, R. Pörtner, and J. Möller, Eds. Berlin, Heidelberg: Springer,
2021, pp. 97–131. doi: https://doi.org/10.1007/10_2020_137.'
mla: Hernández Rodriguez, Tanja, and Björn Frahm. “Digital Seed Train Twins and
Statistical Methods.” Digital Twins Tools and Concepts for Smart Biomanufacturing,
edited by Christoph Herwig et al., vol. 176, Springer, 2021, pp. 97–131, https://doi.org/10.1007/10_2020_137.
short: 'T. Hernández Rodriguez, B. Frahm, in: C. Herwig, R. Pörtner, J. Möller (Eds.),
Digital Twins Tools and Concepts for Smart Biomanufacturing, Springer, Berlin,
Heidelberg, 2021, pp. 97–131.'
ufg: 'Hernández Rodriguez, Tanja/Frahm, Björn: Digital Seed Train Twins and
Statistical Methods, in: Herwig, Christoph/Pörtner, Ralf/Möller, Johannes (Hgg.):
Digital Twins Tools and Concepts for Smart Biomanufacturing, Bd. 176, Berlin,
Heidelberg 2021 (Advances in Biochemical Engineering/Biotechnology), S. 97–131.'
van: 'Hernández Rodriguez T, Frahm B. Digital Seed Train Twins and Statistical Methods.
In: Herwig C, Pörtner R, Möller J, editors. Digital Twins Tools and Concepts for
Smart Biomanufacturing. Berlin, Heidelberg: Springer; 2021. p. 97–131. (Advances
in Biochemical Engineering/Biotechnology; vol. 176).'
date_created: 2020-08-19T07:14:11Z
date_updated: 2023-08-16T06:48:35Z
department:
- _id: DEP4021
doi: https://doi.org/10.1007/10_2020_137
editor:
- first_name: 'Christoph '
full_name: 'Herwig, Christoph '
last_name: Herwig
- first_name: 'Ralf '
full_name: 'Pörtner, Ralf '
last_name: Pörtner
- first_name: 'Johannes '
full_name: 'Möller, Johannes '
last_name: Möller
intvolume: ' 176'
keyword:
- Bayes
- Digital twin
- Parameter estimation
- Seed train
- Uncertainty
language:
- iso: eng
page: 97–131
place: Berlin, Heidelberg
publication: Digital Twins Tools and Concepts for Smart Biomanufacturing
publication_identifier:
eisbn:
- 978-3-030-71660-8
eissn:
- 1616-8542
isbn:
- 978-3-030-71659-2
issn:
- 0724-6145
publication_status: published
publisher: Springer
quality_controlled: '1'
series_title: Advances in Biochemical Engineering/Biotechnology
status: public
title: Digital Seed Train Twins and Statistical Methods
type: book_chapter
user_id: '83781'
volume: 176
year: '2021'
...
---
_id: '13372'
abstract:
- lang: eng
text: "This bachelor thesis explores the adaptive reuse of the former Telekom high-rise
at Kesselbrink in Bielefeld, a vacant modernist office tower awaiting new purpose.
The project proposes a mixed-use transformation that reinterprets the building
as a “city within the city,” inspired by Le Corbusier’s Unité d’Habitation in
Marseille. The existing structure is strategically reduced to its essential load-bearing
concrete frame and floor slabs, preserving the structural skeleton while enabling
radical spatial reconfiguration.\r\nPrefabricated timber modules are inserted
and cantilevered between the reinforced-concrete columns, extending the usable
floor area and introducing a warm, contemporary material contrast to the original
façade. This intervention not only increases density and flexibility but also
redefines the tower’s appearance, giving it a cosmopolitan and forward-looking
architectural identity. The ground floor is conceived as an open and publicly
accessible urban platform, activating the surrounding plaza and strengthening
social interaction. Upper floors are redesigned as affordable and attractive living
spaces for young residents, encouraging a diverse and vibrant community within
the building.\r\nA newly designed park-like rooftop landscape provides recreational
space and panoramic views over the city, enhancing environmental quality and urban
connectivity. By combining structural preservation, modular timber construction,
public programming, and residential reuse, the proposal demonstrates how high-rise
retrofitting can generate social, ecological, and economic value simultaneously.
The project positions adaptive reuse as a viable strategy for sustainable urban
regeneration and architectural longevity."
author:
- first_name: Leon
full_name: Konschake, Leon
id: '84541'
last_name: Konschake
orcid: https://orcid.org/0009-0000-5181-1573
citation:
ama: Konschake L. TreeTower. Technische Hochschule Ostwestfalen-Lippe; 2020.
apa: Konschake, L. (2020). TreeTower. Technische Hochschule Ostwestfalen-Lippe.
bjps: 'Konschake L (2020) TreeTower. Detmolder Schule für Gestaltung:
Technische Hochschule Ostwestfalen-Lippe.'
chicago: 'Konschake, Leon. TreeTower. Detmolder Schule für Gestaltung: Technische
Hochschule Ostwestfalen-Lippe, 2020.'
chicago-de: 'Konschake, Leon. 2020. TreeTower. Detmolder Schule für Gestaltung:
Technische Hochschule Ostwestfalen-Lippe.'
din1505-2-1: 'Konschake, Leon: TreeTower.
Detmolder Schule für Gestaltung : Technische Hochschule Ostwestfalen-Lippe, 2020'
havard: L. Konschake, TreeTower, Technische Hochschule Ostwestfalen-Lippe, Detmolder
Schule für Gestaltung, 2020.
ieee: 'L. Konschake, TreeTower. Detmolder Schule für Gestaltung: Technische
Hochschule Ostwestfalen-Lippe, 2020.'
mla: Konschake, Leon. TreeTower. Technische Hochschule Ostwestfalen-Lippe,
2020.
short: L. Konschake, TreeTower, Technische Hochschule Ostwestfalen-Lippe, Detmolder
Schule für Gestaltung, 2020.
ufg: 'Konschake, Leon: TreeTower, Detmolder Schule für Gestaltung 2020.'
van: 'Konschake L. TreeTower. Detmolder Schule für Gestaltung: Technische Hochschule
Ostwestfalen-Lippe; 2020.'
date_created: 2026-02-12T11:47:28Z
date_updated: 2026-02-20T07:09:36Z
department:
- _id: DEP1600
extern: '1'
keyword:
- Adaptive Reuse
- Mixed-Use Development
- High-Rise Transformation
- City within the City
- Modular Timber Construction
- Prefabrication
- Structural Preservation
- Concrete Skeleton
- Cantilevered Modules
- Urban Regeneration
- Public Ground Floor
- Youth Housing
- Rooftop Landscape
- Sustainability
- Social Impact
- Ecological Design
- Economic Viability
- Cosmopolitan Architecture
language:
- iso: eng
place: Detmolder Schule für Gestaltung
publication_status: published
publisher: Technische Hochschule Ostwestfalen-Lippe
status: public
supervisor:
- first_name: Hans
full_name: Sachs, Hans
id: '64589'
last_name: Sachs
title: TreeTower
type: bachelor_thesis
user_id: '83781'
year: '2020'
...
---
_id: '2394'
abstract:
- lang: eng
text: For the production of biopharmaceuticals a seed train is required to generate
an adequate number of cells for inoculation of the production bioreactor. This
seed train is time- and cost-intensive but offers potential for optimization.
A method and a protocol are described for the seed train mapping, directed modeling
without major effort, and its optimization regarding selected optimization criteria
such as optimal points in time for cell passaging. Furthermore, the method can
also be applied for the set-up of a new seed train, for example for a new cell
line. Although the chapter is directed towards suspension cell lines, the method
is also generally applicable, e.g. for adherent cell lines.
author:
- first_name: Björn
full_name: Frahm, Björn
last_name: Frahm
citation:
ama: 'Frahm B. Seed Train Optimization for Cell Culture. In: Animal Cell Biotechnology.
Vol 1104. Methods in Molecular Biology . Totowa, NJ: Humana Press; 2013:355-367.
doi:10.1007/978-1-62703-733-4_22'
apa: 'Frahm, B. (2013). Seed Train Optimization for Cell Culture. In Animal Cell
Biotechnology (Vol. 1104, pp. 355–367). Totowa, NJ: Humana Press. https://doi.org/10.1007/978-1-62703-733-4_22'
bjps: 'Frahm B (2013) Seed Train Optimization for Cell Culture. Animal
Cell Biotechnology, vol. 1104. Totowa, NJ: Humana Press, pp. 355–367.'
chicago: 'Frahm, Björn. “Seed Train Optimization for Cell Culture.” In Animal
Cell Biotechnology, 1104:355–67. Methods in Molecular Biology . Totowa, NJ:
Humana Press, 2013. https://doi.org/10.1007/978-1-62703-733-4_22.'
chicago-de: 'Frahm, Björn. 2013. Seed Train Optimization for Cell Culture. In: Animal
Cell Biotechnology, 1104:355–367. Methods in Molecular Biology . Totowa, NJ:
Humana Press. doi:10.1007/978-1-62703-733-4_22,
.'
din1505-2-1: 'Frahm, Björn: Seed Train
Optimization for Cell Culture. In: Animal Cell Biotechnology, Methods
in Molecular Biology . Bd. 1104. Totowa, NJ : Humana Press, 2013, S. 355–367'
havard: 'B. Frahm, Seed Train Optimization for Cell Culture, in: Animal Cell Biotechnology,
Humana Press, Totowa, NJ, 2013: pp. 355–367.'
ieee: 'B. Frahm, “Seed Train Optimization for Cell Culture,” in Animal Cell Biotechnology,
vol. 1104, Totowa, NJ: Humana Press, 2013, pp. 355–367.'
mla: Frahm, Björn. “Seed Train Optimization for Cell Culture.” Animal Cell Biotechnology,
vol. 1104, Humana Press, 2013, pp. 355–67, doi:10.1007/978-1-62703-733-4_22.
short: 'B. Frahm, in: Animal Cell Biotechnology, Humana Press, Totowa, NJ, 2013,
pp. 355–367.'
ufg: 'Frahm, Björn (2013): Seed Train Optimization for Cell Culture, in:
Animal Cell Biotechnology (=Methods in Molecular Biology 1104),
Totowa, NJ, S. 355–367.'
van: 'Frahm B. Seed Train Optimization for Cell Culture. In: Animal Cell Biotechnology.
Totowa, NJ: Humana Press; 2013. p. 355–67. (Methods in Molecular Biology ; vol.
1104).'
date_created: 2020-05-19T14:45:43Z
date_updated: 2023-03-15T13:49:39Z
department:
- _id: DEP4021
doi: 10.1007/978-1-62703-733-4_22
intvolume: ' 1104'
keyword:
- Seed train Optimization Modeling Prediction Space-Time-Yield (STY) Systems approach
Bioinformatics Computational biotechnology Suspension Production
language:
- iso: eng
page: 355-367
place: Totowa, NJ
publication: Animal Cell Biotechnology
publication_identifier:
eisbn:
- '9781627037334'
isbn:
- '9781627037327'
issn:
- 1064-3745
- 1940-6029
publication_status: published
publisher: Humana Press
series_title: 'Methods in Molecular Biology '
status: public
title: Seed Train Optimization for Cell Culture
type: book_chapter
user_id: '74004'
volume: 1104
year: 2013
...
---
_id: '10214'
abstract:
- lang: eng
text: For the production of biopharmaceuticals a seed train is required to generate
an adequate number of cells for inoculation of the production bioreactor. This
seed train is time- and cost-intensive but offers potential for optimization.
A method and a protocol are described for the seed train mapping, directed modeling
without major effort, and its optimization regarding selected optimization criteria
such as optimal points in time for cell passaging. Furthermore, the method can
also be applied for the set-up of a new seed train, for example for a new cell
line. Although the chapter is directed towards suspension cell lines, the method
is also generally applicable, e.g. for adherent cell lines.
author:
- first_name: Björn
full_name: Frahm, Björn
id: '45666'
last_name: Frahm
citation:
ama: 'Frahm B. Seed Train Optimization for Cell Culture. In: Pörtner R, ed. Animal
Cell Biotechnology - Methods and Protocols. Vol 1104. 3rd ed. Methods in Molecular
Biology. Humana Press; 2013:355-367. doi:10.1007/978-1-62703-733-4_22'
apa: Frahm, B. (2013). Seed Train Optimization for Cell Culture. In R. Pörtner (Ed.),
Animal Cell Biotechnology - Methods and Protocols (3rd ed., Vol. 1104,
pp. 355–367). Humana Press. https://doi.org/10.1007/978-1-62703-733-4_22
bjps: 'Frahm B (2013) Seed Train Optimization for Cell Culture. In Pörtner
R (ed.), Animal Cell Biotechnology - Methods and Protocols, 3rd ed., vol.
1104. Totowa, NJ: Humana Press, pp. 355–367.'
chicago: 'Frahm, Björn. “Seed Train Optimization for Cell Culture.” In Animal
Cell Biotechnology - Methods and Protocols, edited by Ralf Pörtner, 3rd ed.,
1104:355–67. Methods in Molecular Biology. Totowa, NJ: Humana Press, 2013. https://doi.org/10.1007/978-1-62703-733-4_22.'
chicago-de: 'Frahm, Björn. 2013. Seed Train Optimization for Cell Culture. In: Animal
Cell Biotechnology - Methods and Protocols, hg. von Ralf Pörtner, 1104:355–367.
3. Aufl. Methods in Molecular Biology. Totowa, NJ: Humana Press. doi:10.1007/978-1-62703-733-4_22,
.'
din1505-2-1: 'Frahm, Björn: Seed Train
Optimization for Cell Culture. In: Pörtner,
R. (Hrsg.): Animal Cell Biotechnology - Methods and Protocols, Methods
in Molecular Biology. Bd. 1104. 3. Aufl. Totowa, NJ : Humana Press, 2013,
S. 355–367'
havard: 'B. Frahm, Seed Train Optimization for Cell Culture, in: R. Pörtner (Ed.),
Animal Cell Biotechnology - Methods and Protocols, 3rd ed., Humana Press, Totowa,
NJ, 2013: pp. 355–367.'
ieee: 'B. Frahm, “Seed Train Optimization for Cell Culture,” in Animal Cell Biotechnology
- Methods and Protocols, 3rd ed., vol. 1104, R. Pörtner, Ed. Totowa, NJ: Humana
Press, 2013, pp. 355–367. doi: 10.1007/978-1-62703-733-4_22.'
mla: Frahm, Björn. “Seed Train Optimization for Cell Culture.” Animal Cell Biotechnology
- Methods and Protocols, edited by Ralf Pörtner, 3rd ed., vol. 1104, Humana
Press, 2013, pp. 355–67, https://doi.org/10.1007/978-1-62703-733-4_22.
short: 'B. Frahm, in: R. Pörtner (Ed.), Animal Cell Biotechnology - Methods and
Protocols, 3rd ed., Humana Press, Totowa, NJ, 2013, pp. 355–367.'
ufg: 'Frahm, Björn: Seed Train Optimization for Cell Culture, in: Pörtner,
Ralf (Hg.): Animal Cell Biotechnology - Methods and Protocols, Bd. 1104, Totowa,
NJ 32013 (Methods in Molecular Biology), S. 355–367.'
van: 'Frahm B. Seed Train Optimization for Cell Culture. In: Pörtner R, editor.
Animal Cell Biotechnology - Methods and Protocols. 3rd ed. Totowa, NJ: Humana
Press; 2013. p. 355–67. (Methods in Molecular Biology; vol. 1104).'
date_created: 2023-08-14T17:50:56Z
date_updated: 2023-08-16T06:41:21Z
department:
- _id: DEP4000
doi: 10.1007/978-1-62703-733-4_22
edition: '3'
editor:
- first_name: Ralf
full_name: Pörtner, Ralf
last_name: Pörtner
intvolume: ' 1104'
keyword:
- Seed train
- Optimization
- Modeling
- Prediction
- Space-Time-Yield (STY)
- Systems approach
- Bioinformatics
- Computational biotechnology
- Suspension
- Production
language:
- iso: eng
page: 355–367
place: Totowa, NJ
publication: Animal Cell Biotechnology - Methods and Protocols
publication_identifier:
eisbn:
- 978-1-62703-733-4
eissn:
- 1940-6029
isbn:
- 978-1-62703-732-7
issn:
- 1064-3745
publication_status: published
publisher: Humana Press
series_title: Methods in Molecular Biology
status: public
title: Seed Train Optimization for Cell Culture
type: book_chapter
user_id: '83781'
volume: 1104
year: '2013'
...