---
_id: '12963'
abstract:
- lang: eng
  text: The genus Borrelia comprises vector-borne bacterial pathogens that can severely
    affect human and animal health. Members of the Borrelia burgdorferi sensu lato
    species complex can cause Lyme borreliosis, one of the most common vector-borne
    diseases in the Northern hemisphere. Besides, members of the relapsing fever group
    of spirochetes can cause tick-borne relapsing fever in humans and various febrile
    illnesses in animals in tropical, subtropical and temperate regions. Borrelia
    spp. organisms are fastidious to cultivate and to maintain in vitro, and therefore,
    difficult to work with in the laboratory. Currently, borrelia identification is
    mainly performed using PCR and DNA sequencing methods, which can be complicated/frustrating
    on complex DNA templates and may still be relatively expensive. Alternative techniques
    such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    (MALDI-TOF MS) are not well established for Borrelia spp., although this technique
    is currently one of the most used techniques for rapid identification of bacteria
    in microbiological diagnostic laboratories. This is mainly due to unsatisfactory
    results obtained by use of simple sample preparation techniques and medium-contamination
    obscuring the mass spectra. In addition, comprehensive libraries for Borrelia
    spp. MALDI-TOF MS have yet to be established. In this study, we developed a new
    filter-based chemical extraction technique that allows measurement of high quality
    Borrelia spp. spectra from less than 100,000 bacteria per spot in MALDI-TOF MS.
    We used 49 isolates of 13 different species to produce the largest mass-library
    for Borrelia spp. so far and to validate the protocol. The library was successfully
    established and identifies >96% of used isolates correctly to species level. Cluster
    analysis on the sum spectra was applied to all the different isolates, which resulted
    in tight cluster generation for most species. Comparative analysis of the generated
    cluster to a phylogeny based on concatenated multi-locus sequence typing genes
    provided a surprising homology. Our data demonstrate that the technique described
    here can be used for fast and reliable species and strain typing within the borrelia
    complex.
author:
- first_name: Anna-Cathrine
  full_name: Neumann-Cip, Anna-Cathrine
  last_name: Neumann-Cip
- first_name: Volker
  full_name: Fingerle, Volker
  last_name: Fingerle
- first_name: Gabriele
  full_name: Margos, Gabriele
  last_name: Margos
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Evelyn
  full_name: Overzier, Evelyn
  last_name: Overzier
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Andreas
  full_name: Wieser, Andreas
  last_name: Wieser
citation:
  ama: Neumann-Cip AC, Fingerle V, Margos G, et al. A Novel Rapid Sample Preparation
    Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate
    Differentiation. <i>Frontiers in Microbiology</i>. 2020;11. doi:<a href="https://doi.org/10.3389/fmicb.2020.00690">10.3389/fmicb.2020.00690</a>
  apa: Neumann-Cip, A.-C., Fingerle, V., Margos, G., Straubinger, R. K., Overzier,
    E., Ulrich, S., &#38; Wieser, A. (2020). A Novel Rapid Sample Preparation Method
    for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation.
    <i>Frontiers in Microbiology</i>, <i>11</i>. <a href="https://doi.org/10.3389/fmicb.2020.00690">https://doi.org/10.3389/fmicb.2020.00690</a>
  bjps: <b>Neumann-Cip A-C <i>et al.</i></b> (2020) A Novel Rapid Sample Preparation
    Method for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate
    Differentiation. <i>Frontiers in Microbiology</i> <b>11</b>.
  chicago: Neumann-Cip, Anna-Cathrine, Volker Fingerle, Gabriele Margos, Reinhard
    K. Straubinger, Evelyn Overzier, Sebastian Ulrich, and Andreas Wieser. “A Novel
    Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia Burgdorferi
    Sensu Lato Species and Isolate Differentiation.” <i>Frontiers in Microbiology</i>
    11 (2020). <a href="https://doi.org/10.3389/fmicb.2020.00690">https://doi.org/10.3389/fmicb.2020.00690</a>.
  chicago-de: Neumann-Cip, Anna-Cathrine, Volker Fingerle, Gabriele Margos, Reinhard
    K. Straubinger, Evelyn Overzier, Sebastian Ulrich und Andreas Wieser. 2020. A
    Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi
    Sensu Lato Species and Isolate Differentiation. <i>Frontiers in Microbiology</i>
    11. doi:<a href="https://doi.org/10.3389/fmicb.2020.00690">10.3389/fmicb.2020.00690</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Neumann-Cip, Anna-Cathrine</span>
    ; <span style="font-variant:small-caps;">Fingerle, Volker</span> ; <span style="font-variant:small-caps;">Margos,
    Gabriele</span> ; <span style="font-variant:small-caps;">Straubinger, Reinhard
    K.</span> ; <span style="font-variant:small-caps;">Overzier, Evelyn</span> ; <span
    style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Wieser,
    Andreas</span>: A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits
    Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation. In: <i>Frontiers
    in Microbiology</i> Bd. 11. Lausanne, Frontiers Media SA (2020)'
  havard: A.-C. Neumann-Cip, V. Fingerle, G. Margos, R.K. Straubinger, E. Overzier,
    S. Ulrich, A. Wieser, A Novel Rapid Sample Preparation Method for MALDI-TOF MS
    Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation, Frontiers
    in Microbiology. 11 (2020).
  ieee: 'A.-C. Neumann-Cip <i>et al.</i>, “A Novel Rapid Sample Preparation Method
    for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation,”
    <i>Frontiers in Microbiology</i>, vol. 11, 2020, doi: <a href="https://doi.org/10.3389/fmicb.2020.00690">10.3389/fmicb.2020.00690</a>.'
  mla: Neumann-Cip, Anna-Cathrine, et al. “A Novel Rapid Sample Preparation Method
    for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate Differentiation.”
    <i>Frontiers in Microbiology</i>, vol. 11, 2020, <a href="https://doi.org/10.3389/fmicb.2020.00690">https://doi.org/10.3389/fmicb.2020.00690</a>.
  short: A.-C. Neumann-Cip, V. Fingerle, G. Margos, R.K. Straubinger, E. Overzier,
    S. Ulrich, A. Wieser, Frontiers in Microbiology 11 (2020).
  ufg: '<b>Neumann-Cip, Anna-Cathrine u. a.</b>: A Novel Rapid Sample Preparation
    Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate
    Differentiation, in: <i>Frontiers in Microbiology</i> 11 (2020).'
  van: Neumann-Cip AC, Fingerle V, Margos G, Straubinger RK, Overzier E, Ulrich S,
    et al. A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia
    burgdorferi Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology.
    2020;11.
date_created: 2025-06-15T10:15:09Z
date_updated: 2025-06-18T11:09:40Z
department:
- _id: DEP4010
doi: 10.3389/fmicb.2020.00690
extern: '1'
intvolume: '        11'
keyword:
- Borrelia burgdorferi sensu lato
- MALDI-TOF MS
- typing
- sample preparation
- MALDI-TOF MS library
- strain typing
- automatic identification
language:
- iso: eng
place: Lausanne
publication: Frontiers in Microbiology
publication_identifier:
  eissn:
  - 1664-302X
publication_status: published
publisher: Frontiers Media SA
quality_controlled: '1'
status: public
title: A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi
  Sensu Lato Species and Isolate Differentiation
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2020'
...