[{"issue":"1","quality_controlled":"1","external_id":{"isi":["000539418200006"],"pmid":["31821018"]},"date_updated":"2025-06-26T13:42:15Z","doi":"10.4315/0362-028X.JFP-19-238","type":"scientific_journal_article","publisher":"IAFP","keyword":["Fruits","Inactivation","Murine norovirus","Vapor phase hydrogen peroxide","Vegetables"],"page":"45-51","abstract":[{"text":"Vapor phase hydrogen peroxide (H2O2) can be utilized to inactivate murine norovirus (MNV), a surrogate of human norovirus, on surface areas. However, vapor phase H2O2 inactivation of virus on fruits and vegetables has not been characterized. In this study, MNV was used to determine whether vaporized H2O2 inactivates virus on surfaces of various fruits and vegetables (apples, blueberries, cucumbers, and strawberries). The effect of vapor phase H2O2 decontamination was investigated with two application systems. Plaque assays were performed after virus recovery from untreated and treated fresh produce to compare the quantity of infective MNV. The Mann-Whitney U test was applied to the test results to evaluate the virus titer reductions of treated food samples, with significance set at P <= 0.05. The infective MNV populations were significantly reduced on smooth surfaces by 4.3 log PFU (apples, P < 0.00001) and 4 log PFU or below the detection limit (blueberries, P = 0.0074) by treatment with vapor phase H2O2 (60 min, maximum of 214 ppm of H2O2). Similar treatments of artificially contaminated cucumbers resulted in a virus titer reduction of 1.9 log PFU. Treatment of inoculated strawberries resulted in 0.1and 2.8-log reductions of MNV. However, MNV reduction rates on cucumbers (P = 0.3809) and strawberries (P = 0,7414) were not significant. Triangle tests and color measurements of untreated and treated apples, cucumbers, blueberries, and strawberries revealed no differences in color and consistency after H2O2 treatment. No increase of the H2O2 concentration in treated fruits and vegetables compared with untreated produce was observed. This study reveals for the first time the conditions under which vapor phase H2O2 inactivates MNV on selected fresh fruit and vegetable surfaces.","lang":"eng"}],"department":[{"_id":"DEP4000"}],"user_id":"83781","year":"2020","status":"public","title":"Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide","publication_identifier":{"issn":["0362-028X"],"eissn":["1944-9097"]},"intvolume":"        83","author":[{"last_name":"Becker","id":"12640","full_name":"Becker, Barbara","first_name":"Barbara"},{"last_name":"Dabisch-Ruthe","id":"66516","full_name":"Dabisch-Ruthe, Mareike","first_name":"Mareike","orcid":"https://orcid.org/0009-0008-7644-0826"},{"full_name":"Pfannebecker, Jens","last_name":"Pfannebecker","id":"45690","first_name":"Jens","orcid":"0009-0005-4133-5442"}],"isi":"1","volume":83,"publication_status":"published","pmid":"1","date_created":"2025-04-15T07:59:20Z","language":[{"iso":"eng"}],"citation":{"short":"B. Becker, M. Dabisch-Ruthe, J. Pfannebecker,   Journal of Food Protection  83 (2020) 45–51.","bjps":"<b>Becker B, Dabisch-Ruthe M and Pfannebecker J</b> (2020) Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide. <i>  Journal of food protection </i> <b>83</b>, 45–51.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Becker, Barbara</span> ; <span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Pfannebecker, Jens</span>: Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide. In: <i>  Journal of food protection </i> Bd. 83. Des Moines, Iowa, IAFP (2020), Nr. 1, S. 45–51","apa":"Becker, B., Dabisch-Ruthe, M., &#38; Pfannebecker, J. (2020). Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide. <i>  Journal of Food Protection </i>, <i>83</i>(1), 45–51. <a href=\"https://doi.org/10.4315/0362-028X.JFP-19-238\">https://doi.org/10.4315/0362-028X.JFP-19-238</a>","mla":"Becker, Barbara, et al. “Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide.” <i>  Journal of Food Protection </i>, vol. 83, no. 1, 2020, pp. 45–51, <a href=\"https://doi.org/10.4315/0362-028X.JFP-19-238\">https://doi.org/10.4315/0362-028X.JFP-19-238</a>.","ufg":"<b>Becker, Barbara/Dabisch-Ruthe, Mareike/Pfannebecker, Jens</b>: Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide, in: <i>  Journal of food protection </i> 83 (2020), H. 1,  S. 45–51.","chicago-de":"Becker, Barbara, Mareike Dabisch-Ruthe und Jens Pfannebecker. 2020. Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide. <i>  Journal of food protection </i> 83, Nr. 1: 45–51. doi:<a href=\"https://doi.org/10.4315/0362-028X.JFP-19-238\">10.4315/0362-028X.JFP-19-238</a>, .","chicago":"Becker, Barbara, Mareike Dabisch-Ruthe, and Jens Pfannebecker. “Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide.” <i>  Journal of Food Protection </i> 83, no. 1 (2020): 45–51. <a href=\"https://doi.org/10.4315/0362-028X.JFP-19-238\">https://doi.org/10.4315/0362-028X.JFP-19-238</a>.","ieee":"B. Becker, M. Dabisch-Ruthe, and J. Pfannebecker, “Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide,” <i>  Journal of food protection </i>, vol. 83, no. 1, pp. 45–51, 2020, doi: <a href=\"https://doi.org/10.4315/0362-028X.JFP-19-238\">10.4315/0362-028X.JFP-19-238</a>.","havard":"B. Becker, M. Dabisch-Ruthe, J. Pfannebecker, Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide,   Journal of Food Protection . 83 (2020) 45–51.","van":"Becker B, Dabisch-Ruthe M, Pfannebecker J. Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide.   Journal of food protection . 2020;83(1):45–51.","ama":"Becker B, Dabisch-Ruthe M, Pfannebecker J. Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide. <i>  Journal of food protection </i>. 2020;83(1):45-51. doi:<a href=\"https://doi.org/10.4315/0362-028X.JFP-19-238\">10.4315/0362-028X.JFP-19-238</a>"},"place":"Des Moines, Iowa","_id":"12787","publication":"  Journal of food protection "},{"title":"Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer?","place":"Chichester","citation":{"havard":"I. Esteban‐Cuesta, N. Drees, S. Ulrich, P. Stauch, B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer?, Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech. 98 (2018) 5074–5081.","ieee":"I. Esteban‐Cuesta <i>et al.</i>, “Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer?,” <i>Journal of the science of food and agriculture : incorporating Agri-Biotech</i>, vol. 98, no. 13, pp. 5074–5081, 2018, doi: <a href=\"https://doi.org/10.1002/jsfa.9045\">10.1002/jsfa.9045</a>.","ama":"Esteban‐Cuesta I, Drees N, Ulrich S, et al. Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer? <i>Journal of the science of food and agriculture : incorporating Agri-Biotech</i>. 2018;98(13):5074-5081. doi:<a href=\"https://doi.org/10.1002/jsfa.9045\">10.1002/jsfa.9045</a>","van":"Esteban‐Cuesta I, Drees N, Ulrich S, Stauch P, Sperner B, Schwaiger K, et al. Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer? Journal of the science of food and agriculture : incorporating Agri-Biotech. 2018;98(13):5074–81.","bjps":"<b>Esteban‐Cuesta I <i>et al.</i></b> (2018) Endogenous Microbial Contamination of Melons (<i>Cucumis Melo</i>) from International Trade: An Underestimated Risk for the Consumer? <i>Journal of the science of food and agriculture : incorporating Agri-Biotech</i> <b>98</b>, 5074–5081.","short":"I. Esteban‐Cuesta, N. Drees, S. Ulrich, P. Stauch, B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech 98 (2018) 5074–5081.","mla":"Esteban‐Cuesta, Irene, et al. “Endogenous Microbial Contamination of Melons (<i>Cucumis Melo</i>) from International Trade: An Underestimated Risk for the Consumer?” <i>Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech</i>, vol. 98, no. 13, 2018, pp. 5074–81, <a href=\"https://doi.org/10.1002/jsfa.9045\">https://doi.org/10.1002/jsfa.9045</a>.","apa":"Esteban‐Cuesta, I., Drees, N., Ulrich, S., Stauch, P., Sperner, B., Schwaiger, K., Gareis, M., &#38; Gottschalk, C. (2018). Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer? <i>Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech</i>, <i>98</i>(13), 5074–5081. <a href=\"https://doi.org/10.1002/jsfa.9045\">https://doi.org/10.1002/jsfa.9045</a>","din1505-2-1":"<span style=\"font-variant:small-caps;\">Esteban‐Cuesta, Irene</span> ; <span style=\"font-variant:small-caps;\">Drees, Nathalie</span> ; <span style=\"font-variant:small-caps;\">Ulrich, Sebastian</span> ; <span style=\"font-variant:small-caps;\">Stauch, Peter</span> ; <span style=\"font-variant:small-caps;\">Sperner, Brigitte</span> ; <span style=\"font-variant:small-caps;\">Schwaiger, Karin</span> ; <span style=\"font-variant:small-caps;\">Gareis, Manfred</span> ; <span style=\"font-variant:small-caps;\">Gottschalk, Christoph</span>: Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer? In: <i>Journal of the science of food and agriculture : incorporating Agri-Biotech</i> Bd. 98. Chichester, Wiley (2018), Nr. 13, S. 5074–5081","ufg":"<b>Esteban‐Cuesta, Irene u. a.</b>: Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer?, in: <i>Journal of the science of food and agriculture : incorporating Agri-Biotech</i> 98 (2018), H. 13,  S. 5074–5081.","chicago":"Esteban‐Cuesta, Irene, Nathalie Drees, Sebastian Ulrich, Peter Stauch, Brigitte Sperner, Karin Schwaiger, Manfred Gareis, and Christoph Gottschalk. “Endogenous Microbial Contamination of Melons (<i>Cucumis Melo</i>) from International Trade: An Underestimated Risk for the Consumer?” <i>Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech</i> 98, no. 13 (2018): 5074–81. <a href=\"https://doi.org/10.1002/jsfa.9045\">https://doi.org/10.1002/jsfa.9045</a>.","chicago-de":"Esteban‐Cuesta, Irene, Nathalie Drees, Sebastian Ulrich, Peter Stauch, Brigitte Sperner, Karin Schwaiger, Manfred Gareis und Christoph Gottschalk. 2018. Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk for the consumer? <i>Journal of the science of food and agriculture : incorporating Agri-Biotech</i> 98, Nr. 13: 5074–5081. doi:<a href=\"https://doi.org/10.1002/jsfa.9045\">10.1002/jsfa.9045</a>, ."},"language":[{"iso":"eng"}],"date_created":"2025-06-15T10:28:46Z","volume":98,"publication_status":"published","intvolume":"        98","author":[{"last_name":"Esteban‐Cuesta","full_name":"Esteban‐Cuesta, Irene","first_name":"Irene"},{"first_name":"Nathalie","full_name":"Drees, Nathalie","last_name":"Drees"},{"full_name":"Ulrich, Sebastian","last_name":"Ulrich","id":"85847","first_name":"Sebastian","orcid":"0000-0002-4511-9537"},{"last_name":"Stauch","full_name":"Stauch, Peter","first_name":"Peter"},{"last_name":"Sperner","full_name":"Sperner, Brigitte","first_name":"Brigitte"},{"last_name":"Schwaiger","full_name":"Schwaiger, Karin","first_name":"Karin"},{"last_name":"Gareis","full_name":"Gareis, Manfred","first_name":"Manfred"},{"first_name":"Christoph","full_name":"Gottschalk, Christoph","last_name":"Gottschalk"}],"publication_identifier":{"issn":["0022-5142"],"eissn":["1097-0010"]},"_id":"12974","publication":"Journal of the science of food and agriculture : incorporating Agri-Biotech","doi":"10.1002/jsfa.9045","date_updated":"2025-06-18T11:32:30Z","quality_controlled":"1","issue":"13","publisher":"Wiley","type":"scientific_journal_article","abstract":[{"text":"BACKGROUND\r\nFruits and vegetables have increasingly been related to foodborne outbreaks. Besides surface contamination, a possible internalization of microorganisms into edible parts of plants during growth has already been observed. To examine an actual risk for the consumer, microbial contamination of the rind and pulp of 147 muskmelons from international trade was assessed using cultural and biochemical methods, polymerase chain reaction and matrix-assisted laser desorption/ionization-time of flight mass spectrometry.\r\nRESULTS\r\nOne hundred percent of the rind samples [3.69–8.92 log colony forming units (CFU) g−1] and 89.8% of the pulp samples (maximum load 3.66 log CFU g−1) were microbiologically contaminated. Among the 432 pulp isolates, opportunistic and potentially pathogenic bacteria were identified, mainly Staphylococcus spp. (48.9%), Clostridium spp. (42.9%) and Enterobacteriaceae (27.9%). Salmonella spp., Escherichia coli and isolates of the Bacillus cereus group were found on the rind (1.4%, 0.7% and 42.9%, respectively) and in the pulp (0.7%, 1.4% and 4.7%). Clostridium perfringens was isolated from the rind of seven melons.\r\nCONCLUSION\r\nThe present study revealed a regularly occurring internal contamination of melons. Possible health risks for consumers because of an occurrence of microorganisms in melon pulp should be considered in future food safety assessments. © 2018 Society of Chemical Industry.","lang":"eng"}],"page":"5074-5081","extern":"1","keyword":["foodborne pathogens","Salmonella","Listeria monocytogenes","Enterobacteriaceae","vegetables"],"status":"public","user_id":"83781","year":"2018","department":[{"_id":"DEP4010"}]}]