---
_id: '13233'
abstract:
- lang: eng
  text: 'Atranones are secondary metabolites produced by Stachybotrys chartarum, a
    mold frequently found in water-damaged indoor environments. In contrast to the
    well-characterized and highly toxic macrocyclic trichothecenes, atranones have
    received relatively limited scientific attention. Approximately 60% of S. chartarum
    isolates from indoor environments produce atranones, while 40% form macrocyclic
    trichothecenes. No strain has been shown to produce both, indicating that the
    biosynthetic pathways for these two mycotoxin classes are mutually exclusive.
    Atranones are dolabellane-like diterpenoids synthesized from geranylgeranyl pyrophosphate
    through multiple enzymatic steps encoded by a specific core gene cluster. While
    the genetic structure of this cluster has been elucidated, its regulatory mechanisms
    remain poorly understood. Notably, although atranone-producing S. chartarum strains
    have been isolated from indoor settings, no study has yet confirmed the actual
    production of atranones in indoor environments, leaving the question of real-world
    exposure unresolved. Experimental studies in cell cultures and animal models indicate
    that atranones possess pro-inflammatory and cytotoxic properties, including the
    induction of apoptosis and cell cycle arrest. Atranone Q has demonstrated antitumor
    activity against osteosarcoma cells in vitro, and more recently identified derivatives
    such as stachatranone and stachybatranone have shown preliminary cardioprotective
    effects under ischemic conditions. However, these pharmacological effects remain
    exploratory and require further validation in in vivo models. Major knowledge
    gaps concern the environmental triggers for atranone biosynthesis, their regulation,
    actual presence in built environments, and potential health risks. These areas
    represent key priorities for future research. '
author:
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Jens
  full_name: Pfannebecker, Jens
  id: '45690'
  last_name: Pfannebecker
  orcid: 0009-0005-4133-5442
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Frank
  full_name: Ebel, Frank
  last_name: Ebel
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
citation:
  ama: Dabisch-Ruthe M, Pfannebecker J, Straubinger RK, Ebel F, Ulrich S. Atranone-an
    underestimated secondary metabolite? <i>Mycotoxin Research</i>. Published online
    2025. doi:<a href="https://doi.org/10.1007/s12550-025-00609-x">10.1007/s12550-025-00609-x</a>
  apa: Dabisch-Ruthe, M., Pfannebecker, J., Straubinger, R. K., Ebel, F., &#38; Ulrich,
    S. (2025). Atranone-an underestimated secondary metabolite? <i>Mycotoxin Research</i>.
    <a href="https://doi.org/10.1007/s12550-025-00609-x">https://doi.org/10.1007/s12550-025-00609-x</a>
  bjps: <b>Dabisch-Ruthe M <i>et al.</i></b> (2025) Atranone-an Underestimated Secondary
    Metabolite? <i>Mycotoxin Research</i>.
  chicago: Dabisch-Ruthe, Mareike, Jens Pfannebecker, Reinhard K. Straubinger, Frank
    Ebel, and Sebastian Ulrich. “Atranone-an Underestimated Secondary Metabolite?”
    <i>Mycotoxin Research</i>, 2025. <a href="https://doi.org/10.1007/s12550-025-00609-x">https://doi.org/10.1007/s12550-025-00609-x</a>.
  chicago-de: Dabisch-Ruthe, Mareike, Jens Pfannebecker, Reinhard K. Straubinger,
    Frank Ebel und Sebastian Ulrich. 2025. Atranone-an underestimated secondary metabolite?
    <i>Mycotoxin Research</i>. doi:<a href="https://doi.org/10.1007/s12550-025-00609-x">10.1007/s12550-025-00609-x</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span>
    ; <span style="font-variant:small-caps;">Pfannebecker, Jens</span> ; <span style="font-variant:small-caps;">Straubinger,
    Reinhard K.</span> ; <span style="font-variant:small-caps;">Ebel, Frank</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>: Atranone-an
    underestimated secondary metabolite? In: <i>Mycotoxin Research</i>. Berlin ; Heidelberg,
    Springer (2025)'
  havard: M. Dabisch-Ruthe, J. Pfannebecker, R.K. Straubinger, F. Ebel, S. Ulrich,
    Atranone-an underestimated secondary metabolite?, Mycotoxin Research. (2025).
  ieee: 'M. Dabisch-Ruthe, J. Pfannebecker, R. K. Straubinger, F. Ebel, and S. Ulrich,
    “Atranone-an underestimated secondary metabolite?,” <i>Mycotoxin Research</i>,
    2025, doi: <a href="https://doi.org/10.1007/s12550-025-00609-x">10.1007/s12550-025-00609-x</a>.'
  mla: Dabisch-Ruthe, Mareike, et al. “Atranone-an Underestimated Secondary Metabolite?”
    <i>Mycotoxin Research</i>, 2025, <a href="https://doi.org/10.1007/s12550-025-00609-x">https://doi.org/10.1007/s12550-025-00609-x</a>.
  short: M. Dabisch-Ruthe, J. Pfannebecker, R.K. Straubinger, F. Ebel, S. Ulrich,
    Mycotoxin Research (2025).
  ufg: '<b>Dabisch-Ruthe, Mareike u. a.</b>: Atranone-an underestimated secondary
    metabolite?, in: <i>Mycotoxin Research</i> (2025).'
  van: Dabisch-Ruthe M, Pfannebecker J, Straubinger RK, Ebel F, Ulrich S. Atranone-an
    underestimated secondary metabolite? Mycotoxin Research. 2025;
date_created: 2025-10-01T09:20:35Z
date_updated: 2025-10-06T12:11:00Z
department:
- _id: DEP4010
doi: 10.1007/s12550-025-00609-x
keyword:
- Atranone
- Secondary metabolite
- Stachybotrys
- Stachatranone
- Stachybatranone
language:
- iso: eng
place: Berlin ; Heidelberg
publication: Mycotoxin Research
publication_identifier:
  eissn:
  - 1867-1632
  issn:
  - 0178-7888
publication_status: published
publisher: Springer
status: public
title: Atranone-an underestimated secondary metabolite?
type: scientific_journal_article
user_id: '83781'
year: '2025'
...
---
_id: '12787'
abstract:
- lang: eng
  text: Vapor phase hydrogen peroxide (H2O2) can be utilized to inactivate murine
    norovirus (MNV), a surrogate of human norovirus, on surface areas. However, vapor
    phase H2O2 inactivation of virus on fruits and vegetables has not been characterized.
    In this study, MNV was used to determine whether vaporized H2O2 inactivates virus
    on surfaces of various fruits and vegetables (apples, blueberries, cucumbers,
    and strawberries). The effect of vapor phase H2O2 decontamination was investigated
    with two application systems. Plaque assays were performed after virus recovery
    from untreated and treated fresh produce to compare the quantity of infective
    MNV. The Mann-Whitney U test was applied to the test results to evaluate the virus
    titer reductions of treated food samples, with significance set at P <= 0.05.
    The infective MNV populations were significantly reduced on smooth surfaces by
    4.3 log PFU (apples, P < 0.00001) and 4 log PFU or below the detection limit (blueberries,
    P = 0.0074) by treatment with vapor phase H2O2 (60 min, maximum of 214 ppm of
    H2O2). Similar treatments of artificially contaminated cucumbers resulted in a
    virus titer reduction of 1.9 log PFU. Treatment of inoculated strawberries resulted
    in 0.1and 2.8-log reductions of MNV. However, MNV reduction rates on cucumbers
    (P = 0.3809) and strawberries (P = 0,7414) were not significant. Triangle tests
    and color measurements of untreated and treated apples, cucumbers, blueberries,
    and strawberries revealed no differences in color and consistency after H2O2 treatment.
    No increase of the H2O2 concentration in treated fruits and vegetables compared
    with untreated produce was observed. This study reveals for the first time the
    conditions under which vapor phase H2O2 inactivates MNV on selected fresh fruit
    and vegetable surfaces.
author:
- first_name: Barbara
  full_name: Becker, Barbara
  id: '12640'
  last_name: Becker
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Jens
  full_name: Pfannebecker, Jens
  id: '45690'
  last_name: Pfannebecker
  orcid: 0009-0005-4133-5442
citation:
  ama: Becker B, Dabisch-Ruthe M, Pfannebecker J. Inactivation of Murine Norovirus
    on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide. <i>  Journal
    of food protection </i>. 2020;83(1):45-51. doi:<a href="https://doi.org/10.4315/0362-028X.JFP-19-238">10.4315/0362-028X.JFP-19-238</a>
  apa: Becker, B., Dabisch-Ruthe, M., &#38; Pfannebecker, J. (2020). Inactivation
    of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide.
    <i>  Journal of Food Protection </i>, <i>83</i>(1), 45–51. <a href="https://doi.org/10.4315/0362-028X.JFP-19-238">https://doi.org/10.4315/0362-028X.JFP-19-238</a>
  bjps: <b>Becker B, Dabisch-Ruthe M and Pfannebecker J</b> (2020) Inactivation of
    Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide.
    <i>  Journal of food protection </i> <b>83</b>, 45–51.
  chicago: 'Becker, Barbara, Mareike Dabisch-Ruthe, and Jens Pfannebecker. “Inactivation
    of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide.”
    <i>  Journal of Food Protection </i> 83, no. 1 (2020): 45–51. <a href="https://doi.org/10.4315/0362-028X.JFP-19-238">https://doi.org/10.4315/0362-028X.JFP-19-238</a>.'
  chicago-de: 'Becker, Barbara, Mareike Dabisch-Ruthe und Jens Pfannebecker. 2020.
    Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase
    Hydrogen Peroxide. <i>  Journal of food protection </i> 83, Nr. 1: 45–51. doi:<a
    href="https://doi.org/10.4315/0362-028X.JFP-19-238">10.4315/0362-028X.JFP-19-238</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Becker, Barbara</span> ; <span
    style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span> ; <span style="font-variant:small-caps;">Pfannebecker,
    Jens</span>: Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces
    by Vapor Phase Hydrogen Peroxide. In: <i>  Journal of food protection </i> Bd.
    83. Des Moines, Iowa, IAFP (2020), Nr. 1, S. 45–51'
  havard: B. Becker, M. Dabisch-Ruthe, J. Pfannebecker, Inactivation of Murine Norovirus
    on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide,   Journal of
    Food Protection . 83 (2020) 45–51.
  ieee: 'B. Becker, M. Dabisch-Ruthe, and J. Pfannebecker, “Inactivation of Murine
    Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide,” <i> 
    Journal of food protection </i>, vol. 83, no. 1, pp. 45–51, 2020, doi: <a href="https://doi.org/10.4315/0362-028X.JFP-19-238">10.4315/0362-028X.JFP-19-238</a>.'
  mla: Becker, Barbara, et al. “Inactivation of Murine Norovirus on Fruit and Vegetable
    Surfaces by Vapor Phase Hydrogen Peroxide.” <i>  Journal of Food Protection </i>,
    vol. 83, no. 1, 2020, pp. 45–51, <a href="https://doi.org/10.4315/0362-028X.JFP-19-238">https://doi.org/10.4315/0362-028X.JFP-19-238</a>.
  short: B. Becker, M. Dabisch-Ruthe, J. Pfannebecker,   Journal of Food Protection  83
    (2020) 45–51.
  ufg: '<b>Becker, Barbara/Dabisch-Ruthe, Mareike/Pfannebecker, Jens</b>: Inactivation
    of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide,
    in: <i>  Journal of food protection </i> 83 (2020), H. 1,  S. 45–51.'
  van: Becker B, Dabisch-Ruthe M, Pfannebecker J. Inactivation of Murine Norovirus
    on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide.   Journal of
    food protection . 2020;83(1):45–51.
date_created: 2025-04-15T07:59:20Z
date_updated: 2025-06-26T13:42:15Z
department:
- _id: DEP4000
doi: 10.4315/0362-028X.JFP-19-238
external_id:
  isi:
  - '000539418200006'
  pmid:
  - '31821018'
intvolume: '        83'
isi: '1'
issue: '1'
keyword:
- Fruits
- Inactivation
- Murine norovirus
- Vapor phase hydrogen peroxide
- Vegetables
language:
- iso: eng
page: 45-51
place: Des Moines, Iowa
pmid: '1'
publication: '  Journal of food protection '
publication_identifier:
  eissn:
  - 1944-9097
  issn:
  - 0362-028X
publication_status: published
publisher: IAFP
quality_controlled: '1'
status: public
title: Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase
  Hydrogen Peroxide
type: scientific_journal_article
user_id: '83781'
volume: 83
year: '2020'
...
---
_id: '12988'
abstract:
- lang: eng
  text: "BACKGROUND\r\nBacterial contamination of platelet concentrates (PCs) is still
    a major challenge in transfusion medicine. Different methodologic concepts and
    screening strategies have been developed and investigated concerning their usability.
    We evaluated the feasibility of BacT/ALERT automated culture (BacT/A, bioMérieux)
    with late sampling after 3 days at the earliest.\r\nSTUDY DESIGN AND METHODS\r\nTwenty-four
    bacterial strains isolated from PCs and six relevant strains from reference stocks
    were spiked into apheresis-derived PCs (10-60 colony-forming units [CFU]/bag).
    Sampling was performed after 3 days, and bacterial detection was investigated
    using the two detection methods (BacT/A and BactiFlow [BF], bioMérieux). The maximum
    time-to-result of BacT/A was set to less than 12 hours.\r\nRESULTS\r\nAll medium-
    or high-pathogenic strains are capable of proliferating to high titers, and 100%
    of contaminated samples were detected by BF and BacT/A (6 to ≤12 h incubation);
    lower detection rates of BacT/A were obtained within 6 hours of incubation (≤6
    h: 76.2-93.4%). The majority of low-pathogenic isolates are also capable of growing
    in PCs (89.7%), showing a detection rate of 74.3% for BF versus 54.3% for BacT/A
    (6 to ≤12 h incubation). BacT/A failed to detect bacteria within 6 hours of incubation.
    Certainly, a small number of strains did not grow under PC storage conditions
    and were detectable by BacT/A only with increased detection times.\r\nCONCLUSIONS\r\nLate
    sampling after 3 days at the earliest, combined with reduced BacT/A incubation
    following the negative-to-date concept, offer an alternative opportunity to extend
    the shelf life of PCs from 4 to 5 days in Germany. The sensitivity of BacT/A with
    late sampling is nearly comparable to BF; the time-to-result is considerably longer.\r\n"
author:
- first_name: Tanja
  full_name: Vollmer, Tanja
  last_name: Vollmer
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Melanie
  full_name: Weinstock, Melanie
  last_name: Weinstock
- first_name: Cornelius
  full_name: Knabbe, Cornelius
  last_name: Knabbe
- first_name: Jens
  full_name: Dreier, Jens
  last_name: Dreier
citation:
  ama: Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling
    for automated culture to extend the platelet shelf life to 5 days in Germany.
    <i>Transfusion</i>. 2018;58(7):1654-1664. doi:<a href="https://doi.org/10.1111/trf.14617">10.1111/trf.14617</a>
  apa: Vollmer, T., Dabisch-Ruthe, M., Weinstock, M., Knabbe, C., &#38; Dreier, J.
    (2018). Late sampling for automated culture to extend the platelet shelf life
    to 5 days in Germany. <i>Transfusion</i>, <i>58</i>(7), 1654–1664. <a href="https://doi.org/10.1111/trf.14617">https://doi.org/10.1111/trf.14617</a>
  bjps: <b>Vollmer T <i>et al.</i></b> (2018) Late Sampling for Automated Culture
    to Extend the Platelet Shelf Life to 5 Days in Germany. <i>Transfusion</i> <b>58</b>,
    1654–1664.
  chicago: 'Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe,
    and Jens Dreier. “Late Sampling for Automated Culture to Extend the Platelet Shelf
    Life to 5 Days in Germany.” <i>Transfusion</i> 58, no. 7 (2018): 1654–64. <a href="https://doi.org/10.1111/trf.14617">https://doi.org/10.1111/trf.14617</a>.'
  chicago-de: 'Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius
    Knabbe und Jens Dreier. 2018. Late sampling for automated culture to extend the
    platelet shelf life to 5 days in Germany. <i>Transfusion</i> 58, Nr. 7: 1654–1664.
    doi:<a href="https://doi.org/10.1111/trf.14617">10.1111/trf.14617</a>, .'
  din1505-2-1: '<span style="font-variant:small-caps;">Vollmer, Tanja</span> ; <span
    style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span> ; <span style="font-variant:small-caps;">Weinstock,
    Melanie</span> ; <span style="font-variant:small-caps;">Knabbe, Cornelius</span>
    ; <span style="font-variant:small-caps;">Dreier, Jens</span>: Late sampling for
    automated culture to extend the platelet shelf life to 5 days in Germany. In:
    <i>Transfusion</i> Bd. 58. Oxford [u.a.] , Wiley-Blackwell (2018), Nr. 7, S. 1654–1664'
  havard: T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Late sampling
    for automated culture to extend the platelet shelf life to 5 days in Germany,
    Transfusion. 58 (2018) 1654–1664.
  ieee: 'T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, and J. Dreier, “Late
    sampling for automated culture to extend the platelet shelf life to 5 days in
    Germany,” <i>Transfusion</i>, vol. 58, no. 7, pp. 1654–1664, 2018, doi: <a href="https://doi.org/10.1111/trf.14617">10.1111/trf.14617</a>.'
  mla: Vollmer, Tanja, et al. “Late Sampling for Automated Culture to Extend the Platelet
    Shelf Life to 5 Days in Germany.” <i>Transfusion</i>, vol. 58, no. 7, 2018, pp.
    1654–64, <a href="https://doi.org/10.1111/trf.14617">https://doi.org/10.1111/trf.14617</a>.
  short: T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Transfusion
    58 (2018) 1654–1664.
  ufg: '<b>Vollmer, Tanja u. a.</b>: Late sampling for automated culture to extend
    the platelet shelf life to 5 days in Germany, in: <i>Transfusion</i> 58 (2018),
    H. 7,  S. 1654–1664.'
  van: Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling
    for automated culture to extend the platelet shelf life to 5 days in Germany.
    Transfusion. 2018;58(7):1654–64.
date_created: 2025-06-17T06:55:03Z
date_updated: 2025-06-17T13:48:11Z
department:
- _id: DEP4010
doi: 10.1111/trf.14617
intvolume: '        58'
issue: '7'
language:
- iso: eng
page: 1654-1664
place: 'Oxford [u.a.] '
publication: Transfusion
publication_identifier:
  eissn:
  - 1537-2995
  issn:
  - 0041-1132
publication_status: published
publisher: Wiley-Blackwell
quality_controlled: '1'
status: public
title: Late sampling for automated culture to extend the platelet shelf life to 5
  days in Germany
type: scientific_journal_article
user_id: '83781'
volume: 58
year: '2018'
...
---
_id: '12989'
author:
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Melanie
  full_name: Weinstock, Melanie
  last_name: Weinstock
- first_name: Jens
  full_name: Pfannebecker, Jens
  id: '45690'
  last_name: Pfannebecker
- first_name: Barbara
  full_name: Becker, Barbara
  id: '12640'
  last_name: Becker
citation:
  ama: Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Becker B. <i>Usage of Cold Hydrogen
    Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces.</i>;
    2018. doi:<a href="https://doi.org/10.13140/RG.2.2.12883.08484">10.13140/RG.2.2.12883.08484</a>
  apa: Dabisch-Ruthe, M., Weinstock, M., Pfannebecker, J., &#38; Becker, B. (2018).
    Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus on
    fruit and vegetable surfaces. In <i>26th International ICFMH Conference - FoodMicro
    2018</i>. 26th International ICFMH Conference - FoodMicro 2018, Berlin. <a href="https://doi.org/10.13140/RG.2.2.12883.08484">https://doi.org/10.13140/RG.2.2.12883.08484</a>
  bjps: <b>Dabisch-Ruthe M <i>et al.</i></b> (2018) <i>Usage of Cold Hydrogen Peroxide
    Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces.</i>
    .
  chicago: Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, and Barbara
    Becker. <i>Usage of Cold Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus
    on Fruit and Vegetable Surfaces.</i> <i>26th International ICFMH Conference -
    FoodMicro 2018</i>, 2018. <a href="https://doi.org/10.13140/RG.2.2.12883.08484">https://doi.org/10.13140/RG.2.2.12883.08484</a>.
  chicago-de: Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker und Barbara
    Becker. 2018. <i>Usage of cold hydrogen peroxide vapour for inactivation of murine
    norovirus on fruit and vegetable surfaces.</i> <i>26th International ICFMH Conference
    - FoodMicro 2018</i>. doi:<a href="https://doi.org/10.13140/RG.2.2.12883.08484">10.13140/RG.2.2.12883.08484</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span>
    ; <span style="font-variant:small-caps;">Weinstock, Melanie</span> ; <span style="font-variant:small-caps;">Pfannebecker,
    Jens</span> ; <span style="font-variant:small-caps;">Becker, Barbara</span>: <i>Usage
    of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit
    and vegetable surfaces.</i>, 2018'
  havard: M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, B. Becker, Usage of cold
    hydrogen peroxide vapour for inactivation of murine norovirus on fruit and vegetable
    surfaces., 2018.
  ieee: 'M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, and B. Becker, <i>Usage
    of cold hydrogen peroxide vapour for inactivation of murine norovirus on fruit
    and vegetable surfaces.</i> 2018. doi: <a href="https://doi.org/10.13140/RG.2.2.12883.08484">10.13140/RG.2.2.12883.08484</a>.'
  mla: Dabisch-Ruthe, Mareike, et al. “Usage of Cold Hydrogen Peroxide Vapour for
    Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces.” <i>26th International
    ICFMH Conference - FoodMicro 2018</i>, 2018, <a href="https://doi.org/10.13140/RG.2.2.12883.08484">https://doi.org/10.13140/RG.2.2.12883.08484</a>.
  short: M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, B. Becker, Usage of Cold
    Hydrogen Peroxide Vapour for Inactivation of Murine Norovirus on Fruit and Vegetable
    Surfaces., 2018.
  ufg: '<b>Dabisch-Ruthe, Mareike u. a.</b>: Usage of cold hydrogen peroxide vapour
    for inactivation of murine norovirus on fruit and vegetable surfaces., o. O. 2018.'
  van: Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Becker B. Usage of cold hydrogen
    peroxide vapour for inactivation of murine norovirus on fruit and vegetable surfaces.
    26th International ICFMH Conference - FoodMicro 2018. 2018. 482 p.
conference:
  end_date: 2018-09-06
  location: Berlin
  name: 26th International ICFMH Conference - FoodMicro 2018
  start_date: 2018--09-03
date_created: 2025-06-17T07:05:46Z
date_updated: 2025-06-17T11:49:22Z
department:
- _id: DEP4000
- _id: DEP4010
doi: 10.13140/RG.2.2.12883.08484
language:
- iso: eng
main_file_link:
- open_access: '1'
oa: '1'
page: '482'
publication: 26th International ICFMH Conference - FoodMicro 2018
publication_status: published
related_material:
  link:
  - relation: confirmation
    url: https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00016749/26th-ICFM_FoodMicro2018_Book_of_Abstracts.pdf
status: public
title: Usage of cold hydrogen peroxide vapour for inactivation of murine norovirus
  on fruit and vegetable surfaces.
type: conference_poster
user_id: '83781'
year: '2018'
...
---
_id: '12990'
author:
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Melanie
  full_name: Weinstock, Melanie
  last_name: Weinstock
- first_name: Jens
  full_name: Pfannebecker, Jens
  id: '45690'
  last_name: Pfannebecker
- first_name: Sonja
  full_name: Meyer, Sonja
  id: '12829'
  last_name: Meyer
- first_name: 'Mona '
  full_name: 'Schwetka, Mona '
  last_name: Schwetka
- first_name: Barbara
  full_name: Becker, Barbara
  id: '12640'
  last_name: Becker
citation:
  ama: Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Meyer S, Schwetka M, Becker B.
    <i>Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine
    Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production.</i> Conventus
    Congressmanagement &#38; Marketing GmbH ; 2018. doi:<a href="https://doi.org/10.13140/RG.2.2.22949.41447">10.13140/RG.2.2.22949.41447</a>
  apa: Dabisch-Ruthe, M., Weinstock, M., Pfannebecker, J., Meyer, S., Schwetka, M.,
    &#38; Becker, B. (2018). Application of cold nebulized hydrogen peroxide for inactivation
    of murine norovirus, bacteria and bacteria spores on surfaces in food production.
    In <i>70th Annual Conference of the German Society of Hygiene and Microbiology</i>.
    70th Annual Conference of the German Society of Hygiene and Microbiology, Bochum.
    Conventus Congressmanagement &#38; Marketing GmbH . <a href="https://doi.org/10.13140/RG.2.2.22949.41447">https://doi.org/10.13140/RG.2.2.22949.41447</a>
  bjps: <b>Dabisch-Ruthe M <i>et al.</i></b> (2018) <i>Application of Cold Nebulized
    Hydrogen Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria
    Spores on Surfaces in Food Production.</i> Conventus Congressmanagement &#38;
    Marketing GmbH .
  chicago: Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, Sonja Meyer,
    Mona  Schwetka, and Barbara Becker. <i>Application of Cold Nebulized Hydrogen
    Peroxide for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on
    Surfaces in Food Production.</i> <i>70th Annual Conference of the German Society
    of Hygiene and Microbiology</i>. Conventus Congressmanagement &#38; Marketing
    GmbH , 2018. <a href="https://doi.org/10.13140/RG.2.2.22949.41447">https://doi.org/10.13140/RG.2.2.22949.41447</a>.
  chicago-de: Dabisch-Ruthe, Mareike, Melanie Weinstock, Jens Pfannebecker, Sonja
    Meyer, Mona  Schwetka und Barbara Becker. 2018. <i>Application of cold nebulized
    hydrogen peroxide for inactivation of murine norovirus, bacteria and bacteria
    spores on surfaces in food production.</i> <i>70th Annual Conference of the German
    Society of Hygiene and Microbiology</i>. Conventus Congressmanagement &#38; Marketing
    GmbH . doi:<a href="https://doi.org/10.13140/RG.2.2.22949.41447">10.13140/RG.2.2.22949.41447</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span>
    ; <span style="font-variant:small-caps;">Weinstock, Melanie</span> ; <span style="font-variant:small-caps;">Pfannebecker,
    Jens</span> ; <span style="font-variant:small-caps;">Meyer, Sonja</span> ; <span
    style="font-variant:small-caps;">Schwetka, Mona </span> ; <span style="font-variant:small-caps;">Becker,
    Barbara</span>: <i>Application of cold nebulized hydrogen peroxide for inactivation
    of murine norovirus, bacteria and bacteria spores on surfaces in food production.</i> :
    Conventus Congressmanagement &#38; Marketing GmbH , 2018'
  havard: M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka,
    B. Becker, Application of cold nebulized hydrogen peroxide for inactivation of
    murine norovirus, bacteria and bacteria spores on surfaces in food production.,
    Conventus Congressmanagement &#38; Marketing GmbH , 2018.
  ieee: 'M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka, and
    B. Becker, <i>Application of cold nebulized hydrogen peroxide for inactivation
    of murine norovirus, bacteria and bacteria spores on surfaces in food production.</i>
    Conventus Congressmanagement &#38; Marketing GmbH , 2018. doi: <a href="https://doi.org/10.13140/RG.2.2.22949.41447">10.13140/RG.2.2.22949.41447</a>.'
  mla: Dabisch-Ruthe, Mareike, et al. “Application of Cold Nebulized Hydrogen Peroxide
    for Inactivation of Murine Norovirus, Bacteria and Bacteria Spores on Surfaces
    in Food Production.” <i>70th Annual Conference of the German Society of Hygiene
    and Microbiology</i>, Conventus Congressmanagement &#38; Marketing GmbH , 2018,
    <a href="https://doi.org/10.13140/RG.2.2.22949.41447">https://doi.org/10.13140/RG.2.2.22949.41447</a>.
  short: M. Dabisch-Ruthe, M. Weinstock, J. Pfannebecker, S. Meyer, M. Schwetka, B.
    Becker, Application of Cold Nebulized Hydrogen Peroxide for Inactivation of Murine
    Norovirus, Bacteria and Bacteria Spores on Surfaces in Food Production., Conventus
    Congressmanagement &#38; Marketing GmbH , 2018.
  ufg: '<b>Dabisch-Ruthe, Mareike u. a.</b>: Application of cold nebulized hydrogen
    peroxide for inactivation of murine norovirus, bacteria and bacteria spores on
    surfaces in food production., o. O. 2018.'
  van: Dabisch-Ruthe M, Weinstock M, Pfannebecker J, Meyer S, Schwetka M, Becker B.
    Application of cold nebulized hydrogen peroxide for inactivation of murine norovirus,
    bacteria and bacteria spores on surfaces in food production. 70th Annual Conference
    of the German Society of Hygiene and Microbiology. Conventus Congressmanagement
    &#38; Marketing GmbH ; 2018.
conference:
  end_date: 2018-02-21
  location: Bochum
  name: 70th Annual Conference of the German Society of Hygiene and Microbiology
  start_date: 2018-02-19
date_created: 2025-06-17T07:12:33Z
date_updated: 2025-06-17T08:47:43Z
department:
- _id: DEP4010
doi: 10.13140/RG.2.2.22949.41447
language:
- iso: eng
publication: 70th Annual Conference of the German Society of Hygiene and Microbiology
publication_identifier:
  isbn:
  - 978-3-9816508-6-0
publication_status: published
publisher: 'Conventus Congressmanagement & Marketing GmbH '
related_material:
  link:
  - relation: confirmation
    url: https://www.dghm.org/wp-content/uploads/2018/10/DGHM2018_Abstractband_WebVersion.pdf
status: public
title: Application of cold nebulized hydrogen peroxide for inactivation of murine
  norovirus, bacteria and bacteria spores on surfaces in food production.
type: conference_scientific_abstract
user_id: '83781'
year: '2018'
...
---
_id: '12984'
abstract:
- lang: eng
  text: "Background\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder
    characterized by late onset and progressive calcification of elastic fibers in
    skin, eyes and the cardiovascular system, exemplifying a model for conditions
    characterized by soft tissue calcification.\r\nObjective\r\nThe aim of our study
    was to characterize cellular inorganic pyrophosphate (PPi) homeostasis in PXE.\r\nMethods\r\nGene
    expression of PPi metabolizing enzymes was determined by quantitative real-time
    PCR after incubation up to 21 days with or without addition of Na2HPO4. Extracellular
    and cytosolic PPi concentrations were measured by enzyme-linked bioluminescence
    assay. ALP and ENPP1 activity was determined spectrophotometrically. We further
    established a human cell culture model suitable for investigating PXE and related
    disorders without addition of artificial calcification triggers.\r\nResults\r\nIndependently
    of the experimental conditions, PXE fibroblasts revealed a higher degree of matrix
    calcification. We observed that matrix calcification was associated with altered
    gene expression of PPi metabolizing enzymes in PXE fibroblasts. In this context,
    PXE fibroblasts exhibited significantly higher expression of ALP and OPN and reduced
    mRNA expression and activity of ENPP1. Here, for the first time cytosolic and
    extracellular PPi levels were shown to be strongly reduced in PXE fibroblasts.
    We further showed that PPi concentration in bovine and human sera additives had
    a strong impact on matrix calcification. In a last experimental line, we demonstrated
    that addition of PPi analogs reduced matrix calcification of PXE fibroblasts most
    likely by reducing ALP and OPN mRNA expression, restoring ENPP1 activity and subsequently
    elevating PPi concentrations.\r\nConclusion\r\nThe results of our study along
    with recent findings point to the essential role of PPi as the central regulatory
    metabolites preventing matrix calcification in PXE. But what remains to be determined
    is the underlying molecular mechanism leading to depletion of PPi in PXE. We further
    suggest that supplementation of PPi analogs might counteract pathological calcification
    in PXE and related disorders."
author:
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Patricia
  full_name: Kuzaj, Patricia
  last_name: Kuzaj
- first_name: Christian
  full_name: Götting, Christian
  last_name: Götting
- first_name: Cornelius
  full_name: Knabbe, Cornelius
  last_name: Knabbe
- first_name: Doris
  full_name: Hendig, Doris
  last_name: Hendig
citation:
  ama: Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as
    a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. <i>Journal
    of Dermatological Science</i>. 2014;75(2):109-120. doi:<a href="https://doi.org/10.1016/j.jdermsci.2014.04.015">10.1016/j.jdermsci.2014.04.015</a>
  apa: Dabisch-Ruthe, M., Kuzaj, P., Götting, C., Knabbe, C., &#38; Hendig, D. (2014).
    Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma
    elasticum. <i>Journal of Dermatological Science</i>, <i>75</i>(2), 109–120. <a
    href="https://doi.org/10.1016/j.jdermsci.2014.04.015">https://doi.org/10.1016/j.jdermsci.2014.04.015</a>
  bjps: <b>Dabisch-Ruthe M <i>et al.</i></b> (2014) Pyrophosphates as a Major Inhibitor
    of Matrix Calcification in Pseudoxanthoma Elasticum. <i>Journal of Dermatological
    Science</i> <b>75</b>, 109–120.
  chicago: 'Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius Knabbe,
    and Doris Hendig. “Pyrophosphates as a Major Inhibitor of Matrix Calcification
    in Pseudoxanthoma Elasticum.” <i>Journal of Dermatological Science</i> 75, no.
    2 (2014): 109–20. <a href="https://doi.org/10.1016/j.jdermsci.2014.04.015">https://doi.org/10.1016/j.jdermsci.2014.04.015</a>.'
  chicago-de: 'Dabisch-Ruthe, Mareike, Patricia Kuzaj, Christian Götting, Cornelius
    Knabbe und Doris Hendig. 2014. Pyrophosphates as a major inhibitor of matrix calcification
    in Pseudoxanthoma elasticum. <i>Journal of Dermatological Science</i> 75, Nr.
    2: 109–120. doi:<a href="https://doi.org/10.1016/j.jdermsci.2014.04.015">10.1016/j.jdermsci.2014.04.015</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span>
    ; <span style="font-variant:small-caps;">Kuzaj, Patricia</span> ; <span style="font-variant:small-caps;">Götting,
    Christian</span> ; <span style="font-variant:small-caps;">Knabbe, Cornelius</span>
    ; <span style="font-variant:small-caps;">Hendig, Doris</span>: Pyrophosphates
    as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. In:
    <i>Journal of Dermatological Science</i> Bd. 75. Amsterdam, Elsevier  (2014),
    Nr. 2, S. 109–120'
  havard: M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Pyrophosphates
    as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum, Journal
    of Dermatological Science. 75 (2014) 109–120.
  ieee: 'M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, and D. Hendig, “Pyrophosphates
    as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum,” <i>Journal
    of Dermatological Science</i>, vol. 75, no. 2, pp. 109–120, 2014, doi: <a href="https://doi.org/10.1016/j.jdermsci.2014.04.015">10.1016/j.jdermsci.2014.04.015</a>.'
  mla: Dabisch-Ruthe, Mareike, et al. “Pyrophosphates as a Major Inhibitor of Matrix
    Calcification in Pseudoxanthoma Elasticum.” <i>Journal of Dermatological Science</i>,
    vol. 75, no. 2, 2014, pp. 109–20, <a href="https://doi.org/10.1016/j.jdermsci.2014.04.015">https://doi.org/10.1016/j.jdermsci.2014.04.015</a>.
  short: M. Dabisch-Ruthe, P. Kuzaj, C. Götting, C. Knabbe, D. Hendig, Journal of
    Dermatological Science 75 (2014) 109–120.
  ufg: '<b>Dabisch-Ruthe, Mareike u. a.</b>: Pyrophosphates as a major inhibitor of
    matrix calcification in Pseudoxanthoma elasticum, in: <i>Journal of Dermatological
    Science</i> 75 (2014), H. 2,  S. 109–120.'
  van: Dabisch-Ruthe M, Kuzaj P, Götting C, Knabbe C, Hendig D. Pyrophosphates as
    a major inhibitor of matrix calcification in Pseudoxanthoma elasticum. Journal
    of Dermatological Science. 2014;75(2):109–20.
date_created: 2025-06-17T06:52:59Z
date_updated: 2025-06-17T13:48:41Z
department:
- _id: DEP4010
doi: 10.1016/j.jdermsci.2014.04.015
intvolume: '        75'
issue: '2'
keyword:
- Pseudoxanthoma elasticum
- Calcification
- Pyrophosphate
- Bisphosphonate
- ABCC6
- Tissue nonspecific alkaline phosphate
- Ectonucleotide pyrophosphatase 1
language:
- iso: eng
page: 109-120
place: Amsterdam
publication: Journal of Dermatological Science
publication_identifier:
  eissn:
  - 1873-569X
  issn:
  - 0923-1811
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma
  elasticum
type: scientific_journal_article
user_id: '83781'
volume: 75
year: '2014'
...
---
_id: '12985'
abstract:
- lang: eng
  text: "Objectives\r\nPseudoxanthoma elasticum (PXE) is a rare hereditary disorder
    characterized by progressive calcification and fragmentation of elastic fibers.
    Because of the great clinical variability between PXE patients the involvement
    of modifier genes was recently suggested. Therefore, we investigated the association
    of single nucleotide variants (SNVs) in selected candidate genes known to regulate
    cellular pyrophosphate metabolism.\r\nDesign and methods\r\nWe used RLFP analyses
    to evaluate the distribution of SNVs in alkaline phosphatase (ALP), ectonucleotide
    pyrophosphatase 1 (ENPP1) and ankylosis (ANKH) in DNA samples from 190 German
    PXE patients and 190 age- and sex-matched healthy controls. Statistical analyses
    were performed using Fisher exact test and Bonferroni correction.\r\nResults\r\nThe
    screening revealed three different SNVs in three genes, which were associated
    with PXE. The SNV c.1190-65C > A (rs1780329, minor allele frequency (MAF) patients:
    0.17; controls: 0.11; P = 0.04) in the ALP gene was significantly more frequent
    in PXE patients. Furthermore, PXE was highly associated with ANKH p.A98A genotype
    TT (P = 0.0012), although the MAF was not different between patients and controls.
    After correction for multiple testing according to the Bonferroni method, one
    SNV in the ENPP1 gene (c.313 + 9G > T, rs7773477) remained significantly associated
    with PXE with significantly higher MAF values in the patient cohort (MAF: 0.04
    vs. 0.00; P = 0.0024) and a high association with PXE susceptibility (OR 27.96).\r\nConclusion\r\nPolymorphisms
    in ALP, ENPP1 and ANKH are important genetic risk factors contributing to PXE."
author:
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Alexander
  full_name: Brock, Alexander
  last_name: Brock
- first_name: Patricia
  full_name: Kuzaj, Patricia
  last_name: Kuzaj
- first_name: Peter
  full_name: Charbel Issa, Peter
  last_name: Charbel Issa
- first_name: Christiane
  full_name: Szliska, Christiane
  last_name: Szliska
- first_name: Cornelius
  full_name: Knabbe, Cornelius
  last_name: Knabbe
- first_name: Doris
  full_name: Hendig, Doris
  last_name: Hendig
citation:
  ama: Dabisch-Ruthe M, Brock A, Kuzaj P, et al. Variants in genes encoding pyrophosphate
    metabolizing enzymes are associated with Pseudoxanthoma elasticum. <i>Clinical
    Biochemistry</i>. 2014;47(15):60-67. doi:<a href="https://doi.org/10.1016/j.clinbiochem.2014.07.003">10.1016/j.clinbiochem.2014.07.003</a>
  apa: Dabisch-Ruthe, M., Brock, A., Kuzaj, P., Charbel Issa, P., Szliska, C., Knabbe,
    C., &#38; Hendig, D. (2014). Variants in genes encoding pyrophosphate metabolizing
    enzymes are associated with Pseudoxanthoma elasticum. <i>Clinical Biochemistry</i>,
    <i>47</i>(15), 60–67. <a href="https://doi.org/10.1016/j.clinbiochem.2014.07.003">https://doi.org/10.1016/j.clinbiochem.2014.07.003</a>
  bjps: <b>Dabisch-Ruthe M <i>et al.</i></b> (2014) Variants in Genes Encoding Pyrophosphate
    Metabolizing Enzymes Are Associated with Pseudoxanthoma Elasticum. <i>Clinical
    Biochemistry</i> <b>47</b>, 60–67.
  chicago: 'Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel
    Issa, Christiane Szliska, Cornelius Knabbe, and Doris Hendig. “Variants in Genes
    Encoding Pyrophosphate Metabolizing Enzymes Are Associated with Pseudoxanthoma
    Elasticum.” <i>Clinical Biochemistry</i> 47, no. 15 (2014): 60–67. <a href="https://doi.org/10.1016/j.clinbiochem.2014.07.003">https://doi.org/10.1016/j.clinbiochem.2014.07.003</a>.'
  chicago-de: 'Dabisch-Ruthe, Mareike, Alexander Brock, Patricia Kuzaj, Peter Charbel
    Issa, Christiane Szliska, Cornelius Knabbe und Doris Hendig. 2014. Variants in
    genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma
    elasticum. <i>Clinical Biochemistry</i> 47, Nr. 15: 60–67. doi:<a href="https://doi.org/10.1016/j.clinbiochem.2014.07.003">10.1016/j.clinbiochem.2014.07.003</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span>
    ; <span style="font-variant:small-caps;">Brock, Alexander</span> ; <span style="font-variant:small-caps;">Kuzaj,
    Patricia</span> ; <span style="font-variant:small-caps;">Charbel Issa, Peter</span>
    ; <span style="font-variant:small-caps;">Szliska, Christiane</span> ; <span style="font-variant:small-caps;">Knabbe,
    Cornelius</span> ; <span style="font-variant:small-caps;">Hendig, Doris</span>:
    Variants in genes encoding pyrophosphate metabolizing enzymes are associated with
    Pseudoxanthoma elasticum. In: <i>Clinical Biochemistry</i> Bd. 47. Amsterdam,
    Elsevier (2014), Nr. 15, S. 60–67'
  havard: M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe,
    D. Hendig, Variants in genes encoding pyrophosphate metabolizing enzymes are associated
    with Pseudoxanthoma elasticum, Clinical Biochemistry. 47 (2014) 60–67.
  ieee: 'M. Dabisch-Ruthe <i>et al.</i>, “Variants in genes encoding pyrophosphate
    metabolizing enzymes are associated with Pseudoxanthoma elasticum,” <i>Clinical
    Biochemistry</i>, vol. 47, no. 15, pp. 60–67, 2014, doi: <a href="https://doi.org/10.1016/j.clinbiochem.2014.07.003">10.1016/j.clinbiochem.2014.07.003</a>.'
  mla: Dabisch-Ruthe, Mareike, et al. “Variants in Genes Encoding Pyrophosphate Metabolizing
    Enzymes Are Associated with Pseudoxanthoma Elasticum.” <i>Clinical Biochemistry</i>,
    vol. 47, no. 15, 2014, pp. 60–67, <a href="https://doi.org/10.1016/j.clinbiochem.2014.07.003">https://doi.org/10.1016/j.clinbiochem.2014.07.003</a>.
  short: M. Dabisch-Ruthe, A. Brock, P. Kuzaj, P. Charbel Issa, C. Szliska, C. Knabbe,
    D. Hendig, Clinical Biochemistry 47 (2014) 60–67.
  ufg: '<b>Dabisch-Ruthe, Mareike u. a.</b>: Variants in genes encoding pyrophosphate
    metabolizing enzymes are associated with Pseudoxanthoma elasticum, in: <i>Clinical
    Biochemistry</i> 47 (2014), H. 15,  S. 60–67.'
  van: Dabisch-Ruthe M, Brock A, Kuzaj P, Charbel Issa P, Szliska C, Knabbe C, et
    al. Variants in genes encoding pyrophosphate metabolizing enzymes are associated
    with Pseudoxanthoma elasticum. Clinical Biochemistry. 2014;47(15):60–7.
date_created: 2025-06-17T06:53:30Z
date_updated: 2025-06-17T13:48:52Z
department:
- _id: DEP4010
doi: 10.1016/j.clinbiochem.2014.07.003
intvolume: '        47'
issue: '15'
keyword:
- Pseudoxanthoma elasticum
- Calcification
- Tissue nonspecific alkaline phosphatase
- Ectonucleotide pyrophosphatase 1
- Ankylosis
language:
- iso: eng
page: 60-67
place: Amsterdam
publication: Clinical Biochemistry
publication_identifier:
  eissn:
  - 1873-2933
  issn:
  - 0009-9120
publication_status: published
publisher: Elsevier
status: public
title: Variants in genes encoding pyrophosphate metabolizing enzymes are associated
  with Pseudoxanthoma elasticum
type: scientific_journal_article
user_id: '83781'
volume: 47
year: '2014'
...
---
_id: '12986'
abstract:
- lang: eng
  text: "Background\r\nDysregulations in cholesterol and lipid metabolism have been
    linked to human diseases like hypercholesterolemia, atherosclerosis or the metabolic
    syndrome. Many ABC transporters are involved in trafficking of metabolites derived
    from these pathways. Pseudoxanthoma elasticum (PXE), an autosomal-recessive disease
    caused by ABCC6 mutations, is characterized by atherogenesis and soft tissue calcification.\r\nMethods\r\nIn
    this study we investigated the regulation of cholesterol biosynthesis in human
    dermal fibroblasts from PXE patients and healthy controls.\r\nResults\r\nGene
    expression analysis of 84 targets indicated dysregulations in cholesterol metabolism
    in PXE fibroblasts. Transcript levels of ABCC6 were strongly increased in lipoprotein-deficient
    serum (LPDS) and under serum starvation in healthy controls. For the first time,
    increased HMG CoA reductase activities were found in PXE fibroblasts. We further
    observed strongly elevated transcript and protein levels for the proprotein convertase
    subtilisin/kexin type 9 (PCSK9), as well as a significant reduction in APOE mRNA
    expression in PXE.\r\nConclusion\r\nIncreased cholesterol biosynthesis, elevated
    PCSK9 levels and reduced APOE mRNA expression newly found in PXE fibroblasts could
    enforce atherogenesis and cardiovascular risk in PXE patients. Moreover, the increase
    in ABCC6 expression accompanied by the induction of cholesterol biosynthesis supposes
    a functional role for ABCC6 in human lipoprotein and cholesterol homeostasis."
article_number: '118'
author:
- first_name: Patricia
  full_name: Kuzaj, Patricia
  last_name: Kuzaj
- first_name: Joachim
  full_name: Kuhn, Joachim
  last_name: Kuhn
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Isabel
  full_name: Faust, Isabel
  last_name: Faust
- first_name: Christian
  full_name: Götting, Christian
  last_name: Götting
- first_name: Cornelius
  full_name: Knabbe, Cornelius
  last_name: Knabbe
- first_name: Doris
  full_name: Hendig, Doris
  last_name: Hendig
citation:
  ama: Kuzaj P, Kuhn J, Dabisch-Ruthe M, et al. ABCC6- a new player in cellular cholesterol
    and lipoprotein metabolism? <i>Lipids in Health and Disease</i>. 2014;13(1). doi:<a
    href="https://doi.org/10.1186/1476-511x-13-118">10.1186/1476-511x-13-118</a>
  apa: Kuzaj, P., Kuhn, J., Dabisch-Ruthe, M., Faust, I., Götting, C., Knabbe, C.,
    &#38; Hendig, D. (2014). ABCC6- a new player in cellular cholesterol and lipoprotein
    metabolism? <i>Lipids in Health and Disease</i>, <i>13</i>(1), Article 118. <a
    href="https://doi.org/10.1186/1476-511x-13-118">https://doi.org/10.1186/1476-511x-13-118</a>
  bjps: <b>Kuzaj P <i>et al.</i></b> (2014) ABCC6- a New Player in Cellular Cholesterol
    and Lipoprotein Metabolism? <i>Lipids in Health and Disease</i> <b>13</b>.
  chicago: Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust, Christian
    Götting, Cornelius Knabbe, and Doris Hendig. “ABCC6- a New Player in Cellular
    Cholesterol and Lipoprotein Metabolism?” <i>Lipids in Health and Disease</i> 13,
    no. 1 (2014). <a href="https://doi.org/10.1186/1476-511x-13-118">https://doi.org/10.1186/1476-511x-13-118</a>.
  chicago-de: Kuzaj, Patricia, Joachim Kuhn, Mareike Dabisch-Ruthe, Isabel Faust,
    Christian Götting, Cornelius Knabbe und Doris Hendig. 2014. ABCC6- a new player
    in cellular cholesterol and lipoprotein metabolism? <i>Lipids in Health and Disease</i>
    13, Nr. 1. doi:<a href="https://doi.org/10.1186/1476-511x-13-118">10.1186/1476-511x-13-118</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Kuzaj, Patricia</span> ; <span
    style="font-variant:small-caps;">Kuhn, Joachim</span> ; <span style="font-variant:small-caps;">Dabisch-Ruthe,
    Mareike</span> ; <span style="font-variant:small-caps;">Faust, Isabel</span> ;
    <span style="font-variant:small-caps;">Götting, Christian</span> ; <span style="font-variant:small-caps;">Knabbe,
    Cornelius</span> ; <span style="font-variant:small-caps;">Hendig, Doris</span>:
    ABCC6- a new player in cellular cholesterol and lipoprotein metabolism? In: <i>Lipids
    in Health and Disease</i> Bd. 13. London, Biomed Central  (2014), Nr. 1'
  havard: P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D.
    Hendig, ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?,
    Lipids in Health and Disease. 13 (2014).
  ieee: 'P. Kuzaj <i>et al.</i>, “ABCC6- a new player in cellular cholesterol and
    lipoprotein metabolism?,” <i>Lipids in Health and Disease</i>, vol. 13, no. 1,
    Art. no. 118, 2014, doi: <a href="https://doi.org/10.1186/1476-511x-13-118">10.1186/1476-511x-13-118</a>.'
  mla: Kuzaj, Patricia, et al. “ABCC6- a New Player in Cellular Cholesterol and Lipoprotein
    Metabolism?” <i>Lipids in Health and Disease</i>, vol. 13, no. 1, 118, 2014, <a
    href="https://doi.org/10.1186/1476-511x-13-118">https://doi.org/10.1186/1476-511x-13-118</a>.
  short: P. Kuzaj, J. Kuhn, M. Dabisch-Ruthe, I. Faust, C. Götting, C. Knabbe, D.
    Hendig, Lipids in Health and Disease 13 (2014).
  ufg: '<b>Kuzaj, Patricia u. a.</b>: ABCC6- a new player in cellular cholesterol
    and lipoprotein metabolism?, in: <i>Lipids in Health and Disease</i> 13 (2014),
    H. 1.'
  van: Kuzaj P, Kuhn J, Dabisch-Ruthe M, Faust I, Götting C, Knabbe C, et al. ABCC6-
    a new player in cellular cholesterol and lipoprotein metabolism? Lipids in Health
    and Disease. 2014;13(1).
date_created: 2025-06-17T06:53:58Z
date_updated: 2025-06-17T13:49:15Z
department:
- _id: DEP4010
doi: 10.1186/1476-511x-13-118
intvolume: '        13'
issue: '1'
keyword:
- Pseudoxanthoma elasticum
- ABC transporter
- ABCC6
- Cholesterol biosynthesis
- Atherosclerosis
- HMG CoA reductase
- SREBP2
- PCSK9
- LDLR
- APOE
language:
- iso: eng
place: London
publication: Lipids in Health and Disease
publication_identifier:
  eissn:
  - 1476-511X
publication_status: published
publisher: 'Biomed Central '
quality_controlled: '1'
status: public
title: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?
type: scientific_journal_article
user_id: '83781'
volume: 13
year: '2014'
...
---
_id: '12987'
abstract:
- lang: eng
  text: Mutations in the ABC transporter ABCC6 were recently identified as cause of
    Pseudoxanthoma elasticum (PXE), a rare genetic disorder characterized by progressive
    mineralization of elastic fibers. We used an untargeted metabolic approach to
    identify biochemical differences between human dermal fibroblasts from healthy
    controls and PXE patients in an attempt to find a link between ABCC6 deficiency,
    cellular metabolic alterations and disease pathogenesis. 358 compounds were identified
    by mass spectrometry covering lipids, amino acids, peptides, carbohydrates, nucleotides,
    vitamins and cofactors, xenobiotics and energy metabolites. We found substantial
    differences in glycerophospholipid composition, leucine dipeptides, and polypeptides
    as well as alterations in pantothenate and guanine metabolism to be significantly
    associated with PXE pathogenesis. These findings can be linked to extracellular
    matrix remodeling and increased oxidative stress, which reflect characteristic
    hallmarks of PXE. Our study could facilitate a better understanding of biochemical
    pathways involved in soft tissue mineralization.
article_number: e108336
author:
- first_name: Patricia
  full_name: Kuzaj, Patricia
  last_name: Kuzaj
- first_name: Joachim
  full_name: Kuhn, Joachim
  last_name: Kuhn
- first_name: Ryan D.
  full_name: Michalek, Ryan D.
  last_name: Michalek
- first_name: Edward D.
  full_name: Karoly, Edward D.
  last_name: Karoly
- first_name: Isabel
  full_name: Faust, Isabel
  last_name: Faust
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Cornelius
  full_name: Knabbe, Cornelius
  last_name: Knabbe
- first_name: Doris
  full_name: Hendig, Doris
  last_name: Hendig
citation:
  ama: Kuzaj P, Kuhn J, Michalek RD, et al. Large-Scaled Metabolic Profiling of Human
    Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
    Controls. <i>PLOS ONE / Public Library of Science </i>. 2014;9(9). doi:<a href="https://doi.org/10.1371/journal.pone.0108336">10.1371/journal.pone.0108336</a>
  apa: Kuzaj, P., Kuhn, J., Michalek, R. D., Karoly, E. D., Faust, I., Dabisch-Ruthe,
    M., Knabbe, C., &#38; Hendig, D. (2014). Large-Scaled Metabolic Profiling of Human
    Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
    Controls. <i>PLOS ONE / Public Library of Science </i>, <i>9</i>(9), Article e108336.
    <a href="https://doi.org/10.1371/journal.pone.0108336">https://doi.org/10.1371/journal.pone.0108336</a>
  bjps: <b>Kuzaj P <i>et al.</i></b> (2014) Large-Scaled Metabolic Profiling of Human
    Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy
    Controls. <i>PLOS ONE / Public Library of Science </i> <b>9</b>.
  chicago: Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel
    Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe, and Doris Hendig. “Large-Scaled
    Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
    Patients and Healthy Controls.” <i>PLOS ONE / Public Library of Science </i> 9,
    no. 9 (2014). <a href="https://doi.org/10.1371/journal.pone.0108336">https://doi.org/10.1371/journal.pone.0108336</a>.
  chicago-de: Kuzaj, Patricia, Joachim Kuhn, Ryan D. Michalek, Edward D. Karoly, Isabel
    Faust, Mareike Dabisch-Ruthe, Cornelius Knabbe und Doris Hendig. 2014. Large-Scaled
    Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
    Patients and Healthy Controls. <i>PLOS ONE / Public Library of Science </i> 9,
    Nr. 9. doi:<a href="https://doi.org/10.1371/journal.pone.0108336">10.1371/journal.pone.0108336</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Kuzaj, Patricia</span> ; <span
    style="font-variant:small-caps;">Kuhn, Joachim</span> ; <span style="font-variant:small-caps;">Michalek,
    Ryan D.</span> ; <span style="font-variant:small-caps;">Karoly, Edward D.</span>
    ; <span style="font-variant:small-caps;">Faust, Isabel</span> ; <span style="font-variant:small-caps;">Dabisch-Ruthe,
    Mareike</span> ; <span style="font-variant:small-caps;">Knabbe, Cornelius</span>
    ; <span style="font-variant:small-caps;">Hendig, Doris</span>: Large-Scaled Metabolic
    Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients
    and Healthy Controls. In: <i>PLOS ONE / Public Library of Science </i> Bd. 9.
    San Francisco, California, US, Public Library of Science (PLoS) (2014), Nr. 9'
  havard: P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe,
    C. Knabbe, D. Hendig, Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts
    Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls, PLOS ONE
    / Public Library of Science . 9 (2014).
  ieee: 'P. Kuzaj <i>et al.</i>, “Large-Scaled Metabolic Profiling of Human Dermal
    Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls,”
    <i>PLOS ONE / Public Library of Science </i>, vol. 9, no. 9, Art. no. e108336,
    2014, doi: <a href="https://doi.org/10.1371/journal.pone.0108336">10.1371/journal.pone.0108336</a>.'
  mla: Kuzaj, Patricia, et al. “Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts
    Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls.” <i>PLOS
    ONE / Public Library of Science </i>, vol. 9, no. 9, e108336, 2014, <a href="https://doi.org/10.1371/journal.pone.0108336">https://doi.org/10.1371/journal.pone.0108336</a>.
  short: P. Kuzaj, J. Kuhn, R.D. Michalek, E.D. Karoly, I. Faust, M. Dabisch-Ruthe,
    C. Knabbe, D. Hendig, PLOS ONE / Public Library of Science  9 (2014).
  ufg: '<b>Kuzaj, Patricia u. a.</b>: Large-Scaled Metabolic Profiling of Human Dermal
    Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls,
    in: <i>PLOS ONE / Public Library of Science </i> 9 (2014), H. 9.'
  van: Kuzaj P, Kuhn J, Michalek RD, Karoly ED, Faust I, Dabisch-Ruthe M, et al. Large-Scaled
    Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum
    Patients and Healthy Controls. PLOS ONE / Public Library of Science . 2014;9(9).
date_created: 2025-06-17T06:54:30Z
date_updated: 2025-06-17T13:49:26Z
department:
- _id: DEP4010
doi: 10.1371/journal.pone.0108336
intvolume: '         9'
issue: '9'
language:
- iso: eng
place: San Francisco, California, US
publication: 'PLOS ONE / Public Library of Science '
publication_identifier:
  eissn:
  - 1932-6203
publication_status: published
publisher: Public Library of Science (PLoS)
quality_controlled: '1'
status: public
title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma
  Elasticum Patients and Healthy Controls
type: scientific_journal_article
user_id: '83781'
volume: 9
year: '2014'
...
---
_id: '12983'
abstract:
- lang: eng
  text: "Background\r\nA broad spectrum of pathogens is causative for respiratory
    tract infections, but symptoms are mostly similar. Therefore, the identification
    of the causative viruses and bacteria is only feasible using multiplex PCR or
    several monoplex PCR tests in parallel.\r\nMethods\r\nThe analytical sensitivity
    of three multiplex PCR assays, RespiFinder-19, RespiFinder-SMART-22 and xTAG-Respiratory-Virus-Panel-Fast-Assay
    (RVP), were compared to monoplex real-time PCR with quantified standardized control
    material. All assays include the most common respiratory pathogens.\r\nResults\r\nTo
    compare the analytical sensitivity of the multiplex assays, samples were inoculated
    with 13 different quantified viruses in the range of 101 to 105 copies/ml. Concordant
    results were received for rhinovirus, whereas the RVP detected influenzavirus,
    RSV and hMPV more frequently in low concentrations. The RespiFinder-19 and the
    RespiFinder-SMART-22 showed a higher analytical sensitivity for adenoviruses and
    coronaviruses, whereas the RVP was incapable to detect adenovirus and coronavirus
    in concentrations of 104 copies/ml. The RespiFinder-19 and RespiFinder-SMART-22A
    did not detect influenzaviruses (104 copies/ml) and RSV (103 copies/ml). The detection
    of all 13 viruses in one sample was only achieved using monoplex PCR. To analyze
    possible competitive amplification reactions between the different viruses, samples
    were further inoculated with only 4 different viruses in one sample. Compared
    to the detection of 13 viruses in parallel, only a few differences were found.\r\nThe
    incidence of respiratory viruses was compared in tracheal secretion (TS) samples
    (n = 100) of mechanically ventilated patients in winter (n = 50) and summer (n = 50).
    In winter, respiratory viruses were detected in 32 TS samples (64%) by RespiFinder-19,
    whereas the detection rate with RVP was only 22%. The most frequent viruses were
    adenovirus (32%) and PIV-2 (20%). Multiple infections were detected in 16 TS samples
    (32%) by RespiFinder-19. Fewer infections were found in summer (RespiFinder-19:
    20%; RVP: 6%). All positive results were verified using monoplex PCR.\r\nConclusions\r\nMultiplex
    PCR tests have a broad spectrum of pathogens to test at a time. Analysis of multiple
    inoculated samples revealed a different focus of the detected virus types by the
    three assays. Analysis of clinical samples showed a high concordance of detected
    viruses by the RespiFinder-19 compared to monoplex tests."
article_number: '163'
author:
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Tanja
  full_name: Vollmer, Tanja
  last_name: Vollmer
- first_name: Ortwin
  full_name: Adams, Ortwin
  last_name: Adams
- first_name: Cornelius
  full_name: Knabbe, Cornelius
  last_name: Knabbe
- first_name: Jens
  full_name: Dreier, Jens
  last_name: Dreier
citation:
  ama: 'Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three
    multiplex PCR assays for the detection of respiratory viral infections: evaluation
    of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
    SMART 22 assay. <i>BMC Infectious Diseases</i>. 2012;12(1). doi:<a href="https://doi.org/10.1186/1471-2334-12-163">10.1186/1471-2334-12-163</a>'
  apa: 'Dabisch-Ruthe, M., Vollmer, T., Adams, O., Knabbe, C., &#38; Dreier, J. (2012).
    Comparison of three multiplex PCR assays for the detection of respiratory viral
    infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder
    19 assay and RespiFinder SMART 22 assay. <i>BMC Infectious Diseases</i>, <i>12</i>(1),
    Article 163. <a href="https://doi.org/10.1186/1471-2334-12-163">https://doi.org/10.1186/1471-2334-12-163</a>'
  bjps: '<b>Dabisch-Ruthe M <i>et al.</i></b> (2012) Comparison of Three Multiplex
    PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG
    Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART
    22 Assay. <i>BMC Infectious Diseases</i> <b>12</b>.'
  chicago: 'Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe,
    and Jens Dreier. “Comparison of Three Multiplex PCR Assays for the Detection of
    Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast
    Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” <i>BMC Infectious
    Diseases</i> 12, no. 1 (2012). <a href="https://doi.org/10.1186/1471-2334-12-163">https://doi.org/10.1186/1471-2334-12-163</a>.'
  chicago-de: 'Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe
    und Jens Dreier. 2012. Comparison of three multiplex PCR assays for the detection
    of respiratory viral infections: evaluation of xTAG respiratory virus panel fast
    assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. <i>BMC Infectious
    Diseases</i> 12, Nr. 1. doi:<a href="https://doi.org/10.1186/1471-2334-12-163">10.1186/1471-2334-12-163</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span>
    ; <span style="font-variant:small-caps;">Vollmer, Tanja</span> ; <span style="font-variant:small-caps;">Adams,
    Ortwin</span> ; <span style="font-variant:small-caps;">Knabbe, Cornelius</span>
    ; <span style="font-variant:small-caps;">Dreier, Jens</span>: Comparison of three
    multiplex PCR assays for the detection of respiratory viral infections: evaluation
    of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
    SMART 22 assay. In: <i>BMC Infectious Diseases</i> Bd. 12. Berlin ; Heidelberg,
    Springer (2012), Nr. 1'
  havard: 'M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, Comparison
    of three multiplex PCR assays for the detection of respiratory viral infections:
    evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and
    RespiFinder SMART 22 assay, BMC Infectious Diseases. 12 (2012).'
  ieee: 'M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, and J. Dreier, “Comparison
    of three multiplex PCR assays for the detection of respiratory viral infections:
    evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and
    RespiFinder SMART 22 assay,” <i>BMC Infectious Diseases</i>, vol. 12, no. 1, Art.
    no. 163, 2012, doi: <a href="https://doi.org/10.1186/1471-2334-12-163">10.1186/1471-2334-12-163</a>.'
  mla: 'Dabisch-Ruthe, Mareike, et al. “Comparison of Three Multiplex PCR Assays for
    the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory
    Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.”
    <i>BMC Infectious Diseases</i>, vol. 12, no. 1, 163, 2012, <a href="https://doi.org/10.1186/1471-2334-12-163">https://doi.org/10.1186/1471-2334-12-163</a>.'
  short: M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, BMC Infectious
    Diseases 12 (2012).
  ufg: '<b>Dabisch-Ruthe, Mareike u. a.</b>: Comparison of three multiplex PCR assays
    for the detection of respiratory viral infections: evaluation of xTAG respiratory
    virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, in:
    <i>BMC Infectious Diseases</i> 12 (2012), H. 1.'
  van: 'Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three
    multiplex PCR assays for the detection of respiratory viral infections: evaluation
    of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder
    SMART 22 assay. BMC Infectious Diseases. 2012;12(1).'
date_created: 2025-06-17T06:52:13Z
date_updated: 2025-06-17T13:49:03Z
department:
- _id: DEP4010
doi: 10.1186/1471-2334-12-163
intvolume: '        12'
issue: '1'
keyword:
- Respiratory Virus
- Elution Volume
- Multiplex Assay
- Multiple Infection
- Bocavirus
language:
- iso: eng
place: Berlin ; Heidelberg
publication: BMC Infectious Diseases
publication_identifier:
  eissn:
  - 1471-2334
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: 'Comparison of three multiplex PCR assays for the detection of respiratory
  viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder
  19 assay and RespiFinder SMART 22 assay'
type: scientific_journal_article
user_id: '83781'
volume: 12
year: '2012'
...
---
_id: '12982'
abstract:
- lang: eng
  text: Contaminated food is a significant vehicle for human norovirus transmission.
    The present study determined the effect of physicochemical treatments on the tenacity
    of infective human norovirus genogroup II in selected foods. Artificially contaminated
    produce was subjected to a number of processes used by the food industry for preservation
    and by the consumer for storage and preparation. Virus recovery was carried out
    by using ultrafiltration and was monitored by using bacteriophage MS2 as an internal
    process control. Norovirus was quantified by using monoplex one-step TaqMan real-time
    reverse transcription (RT)-PCR and an external standard curve based on recombinant
    RNA standards. An RNase pretreatment step was used to avoid false-positive PCR
    results caused by accessible RNA, which allowed detection of intact virus particles.
    Significant reductions in titers were obtained with heat treatments usually applied
    by consumers for food preparation (baking, cooking, roasting). Generally, processes
    used for preservation and storage, such as cooling, freezing, acidification (≥pH
    4.5), and moderate heat treatments (pasteurization), appear to be insufficient
    to inactivate norovirus within a food matrix or on the surface of food. Besides
    data for persistence in processed food, comparable data for individual matrix-specific
    protective effects, recovery rates, and inhibitory effects on the PCRs were obtained
    in this study. The established procedure might be used for other noncultivable
    enteric RNA viruses that are connected to food-borne diseases. The data obtained
    in this study may also help optimize the process for inactivation of norovirus
    in food by adjusting food processing technologies and may promote the development
    of risk assessment systems in order to improve consumer protection.
author:
- first_name: Sascha
  full_name: Mormann, Sascha
  last_name: Mormann
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Barbara
  full_name: Becker, Barbara
  id: '12640'
  last_name: Becker
citation:
  ama: Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on
    the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
    Foods. <i>  Applied and environmental microbiology / American Society for Microbiology</i>.
    2009;76(2):536-545. doi:<a href="https://doi.org/10.1128/aem.01797-09">10.1128/aem.01797-09</a>
  apa: Mormann, S., Dabisch-Ruthe, M., &#38; Becker, B. (2009). Effects of Technological
    Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally
    Contaminated Foods. <i>  Applied and Environmental Microbiology / American Society
    for Microbiology</i>, <i>76</i>(2), 536–545. <a href="https://doi.org/10.1128/aem.01797-09">https://doi.org/10.1128/aem.01797-09</a>
  bjps: <b>Mormann S, Dabisch-Ruthe M and Becker B</b> (2009) Effects of Technological
    Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally
    Contaminated Foods. <i>  Applied and environmental microbiology / American Society
    for Microbiology</i> <b>76</b>, 536–545.
  chicago: 'Mormann, Sascha, Mareike Dabisch-Ruthe, and Barbara Becker. “Effects of
    Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
    II in Experimentally Contaminated Foods.” <i>  Applied and Environmental Microbiology
    / American Society for Microbiology</i> 76, no. 2 (2009): 536–45. <a href="https://doi.org/10.1128/aem.01797-09">https://doi.org/10.1128/aem.01797-09</a>.'
  chicago-de: 'Mormann, Sascha, Mareike Dabisch-Ruthe und Barbara Becker. 2009. Effects
    of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
    II in Experimentally Contaminated Foods. <i>  Applied and environmental microbiology
    / American Society for Microbiology</i> 76, Nr. 2: 536–545. doi:<a href="https://doi.org/10.1128/aem.01797-09">10.1128/aem.01797-09</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Mormann, Sascha</span> ; <span
    style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span> ; <span style="font-variant:small-caps;">Becker,
    Barbara</span>: Effects of Technological Processes on the Tenacity and Inactivation
    of Norovirus Genogroup II in Experimentally Contaminated Foods. In: <i>  Applied
    and environmental microbiology / American Society for Microbiology</i> Bd. 76.
    Washington, DC [u.a.], American Society for Microbiology (2009), Nr. 2, S. 536–545'
  havard: S. Mormann, M. Dabisch-Ruthe, B. Becker, Effects of Technological Processes
    on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
    Foods,   Applied and Environmental Microbiology / American Society for Microbiology.
    76 (2009) 536–545.
  ieee: 'S. Mormann, M. Dabisch-Ruthe, and B. Becker, “Effects of Technological Processes
    on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
    Foods,” <i>  Applied and environmental microbiology / American Society for Microbiology</i>,
    vol. 76, no. 2, pp. 536–545, 2009, doi: <a href="https://doi.org/10.1128/aem.01797-09">10.1128/aem.01797-09</a>.'
  mla: Mormann, Sascha, et al. “Effects of Technological Processes on the Tenacity
    and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods.”
    <i>  Applied and Environmental Microbiology / American Society for Microbiology</i>,
    vol. 76, no. 2, 2009, pp. 536–45, <a href="https://doi.org/10.1128/aem.01797-09">https://doi.org/10.1128/aem.01797-09</a>.
  short: S. Mormann, M. Dabisch-Ruthe, B. Becker,   Applied and Environmental Microbiology
    / American Society for Microbiology 76 (2009) 536–545.
  ufg: '<b>Mormann, Sascha/Dabisch-Ruthe, Mareike/Becker, Barbara</b>: Effects of
    Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup
    II in Experimentally Contaminated Foods, in: <i>  Applied and environmental microbiology
    / American Society for Microbiology</i> 76 (2009), H. 2,  S. 536–545.'
  van: Mormann S, Dabisch-Ruthe M, Becker B. Effects of Technological Processes on
    the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated
    Foods.   Applied and environmental microbiology / American Society for Microbiology.
    2009;76(2):536–45.
date_created: 2025-06-17T06:26:59Z
date_updated: 2025-06-17T13:49:35Z
department:
- _id: DEP4010
doi: 10.1128/aem.01797-09
intvolume: '        76'
issue: '2'
language:
- iso: eng
page: 536-545
place: Washington, DC [u.a.]
publication: '  Applied and environmental microbiology / American Society for Microbiology'
publication_identifier:
  eissn:
  - 1098-5336
  issn:
  - '0099-2240 '
publication_status: published
publisher: American Society for Microbiology
quality_controlled: '1'
status: public
title: Effects of Technological Processes on the Tenacity and Inactivation of Norovirus
  Genogroup II in Experimentally Contaminated Foods
type: scientific_journal_article
user_id: '83781'
volume: 76
year: '2009'
...