[{"intvolume":"       424","keyword":["Biodiesel aging","Epoxide-dependent oligomerization","Acetic acid","Identification of aging products","Kinetics of aging products"],"quality_controlled":"1","_id":"13671","year":"2026","type":"scientific_journal_article","article_number":"139339","date_created":"2026-04-07T12:49:23Z","author":[{"first_name":"Julian","last_name":"Türck","full_name":"Türck, Julian"},{"full_name":"Funke, Carsten","last_name":"Funke","first_name":"Carsten","id":"74167"},{"last_name":"Schmitt","first_name":"Fabian","full_name":"Schmitt, Fabian"},{"full_name":"Schneider, Jan","last_name":"Schneider","first_name":"Jan","orcid":"0000-0001-6401-8873","id":"13209"},{"id":"12231","full_name":"Danneel, Hans-Jürgen","last_name":"Danneel","first_name":"Hans-Jürgen"},{"full_name":"Türck, Ralf","first_name":"Ralf","last_name":"Türck"},{"first_name":"Wolfgang","last_name":"Ruck","full_name":"Ruck, Wolfgang"},{"id":"68870","full_name":"Krahl, Jürgen","last_name":"Krahl","first_name":"Jürgen"}],"abstract":[{"text":"The aging of biodiesel proceeds via multiple pathways, with oligomerization playing a central role. In this study, we investigated an epoxide-dependent oligomerization pathway, which had previously only been postulated. Using methyl oleate (C18:1) as a model monounsaturated fatty acid and acetic acid as a representative, reactive nucleophile and known biodiesel aging product with a suitable boiling point, oligomeric products were identified by size-exclusion chromatography (SEC) coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QTOF-MS). The concentration of 20 wt% was chosen to ensure a measurable kinetic effect while maintaining stable reaction conditions. Furthermore, the role of methyl oleate during biodiesel aging was addressed. Time-resolved analysis confirmed the proposed sequential order of reactions. It was pointed out that elimination reactions may occur. The data support the formation of epoxides, despite the isobaric overlap with ketones, and show that hydroxyl intermediates undergo esterification and etherification. Moreover, experiments with pure C18:1 demonstrated that acetic acid–derived oligomers are generated. Under Rancimat conditions, addition of 20 wt% acetic acid resulted in an approximately 1.1 – 2.6 increase in product yield. Kinetic analysis revealed structure-dependent formation and decay behavior of the aging products, with slightly faster epoxidation and shifted product distributions toward higher oligomeric species in the presence of acetic acid. Reactive intermediates were consumed more rapidly than oligomeric species and all decay processes followed apparent second-order kinetics. These findings provide direct experimental evidence for the involvement of epoxide-dependent pathways in biodiesel aging.","lang":"eng"}],"publisher":"Elsevier","doi":"10.1016/j.fuel.2026.139339","date_updated":"2026-04-08T12:55:00Z","publication_identifier":{"eissn":["1873-7153"],"issn":["0016-2361"]},"title":"Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate","publication":"Fuel : the science and technology of fuel and energy ","status":"public","language":[{"iso":"eng"}],"user_id":"83781","citation":{"ufg":"<b>Türck, Julian u. a.</b>: Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate, in: <i>Fuel : the science and technology of fuel and energy </i> 424 (2026).","ieee":"J. Türck <i>et al.</i>, “Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate,” <i>Fuel : the science and technology of fuel and energy </i>, vol. 424, Art. no. 139339, 2026, doi: <a href=\"https://doi.org/10.1016/j.fuel.2026.139339\">10.1016/j.fuel.2026.139339</a>.","van":"Türck J, Funke C, Schmitt F, Schneider J, Danneel HJ, Türck R, et al. Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate. Fuel : the science and technology of fuel and energy . 2026;424.","short":"J. Türck, C. Funke, F. Schmitt, J. Schneider, H.-J. Danneel, R. Türck, W. Ruck, J. Krahl, Fuel : The Science and Technology of Fuel and Energy  424 (2026).","havard":"J. Türck, C. Funke, F. Schmitt, J. Schneider, H.-J. Danneel, R. Türck, W. Ruck, J. Krahl, Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate, Fuel : The Science and Technology of Fuel and Energy . 424 (2026).","bjps":"<b>Türck J <i>et al.</i></b> (2026) Kinetic Study and Confirmation of Epoxide-Dependent Oligomerization of Methyl Oleate. <i>Fuel : the science and technology of fuel and energy </i> <b>424</b>.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Türck, Julian</span> ; <span style=\"font-variant:small-caps;\">Funke, Carsten</span> ; <span style=\"font-variant:small-caps;\">Schmitt, Fabian</span> ; <span style=\"font-variant:small-caps;\">Schneider, Jan</span> ; <span style=\"font-variant:small-caps;\">Danneel, Hans-Jürgen</span> ; <span style=\"font-variant:small-caps;\">Türck, Ralf</span> ; <span style=\"font-variant:small-caps;\">Ruck, Wolfgang</span> ; <span style=\"font-variant:small-caps;\">Krahl, Jürgen</span>: Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate. In: <i>Fuel : the science and technology of fuel and energy </i> Bd. 424. Amsterdam [u.a.], Elsevier (2026)","mla":"Türck, Julian, et al. “Kinetic Study and Confirmation of Epoxide-Dependent Oligomerization of Methyl Oleate.” <i>Fuel : The Science and Technology of Fuel and Energy </i>, vol. 424, 139339, 2026, <a href=\"https://doi.org/10.1016/j.fuel.2026.139339\">https://doi.org/10.1016/j.fuel.2026.139339</a>.","chicago-de":"Türck, Julian, Carsten Funke, Fabian Schmitt, Jan Schneider, Hans-Jürgen Danneel, Ralf Türck, Wolfgang Ruck und Jürgen Krahl. 2026. Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate. <i>Fuel : the science and technology of fuel and energy </i> 424. doi:<a href=\"https://doi.org/10.1016/j.fuel.2026.139339\">10.1016/j.fuel.2026.139339</a>, .","apa":"Türck, J., Funke, C., Schmitt, F., Schneider, J., Danneel, H.-J., Türck, R., Ruck, W., &#38; Krahl, J. (2026). Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate. <i>Fuel : The Science and Technology of Fuel and Energy </i>, <i>424</i>, Article 139339. <a href=\"https://doi.org/10.1016/j.fuel.2026.139339\">https://doi.org/10.1016/j.fuel.2026.139339</a>","ama":"Türck J, Funke C, Schmitt F, et al. Kinetic study and confirmation of epoxide-dependent oligomerization of methyl oleate. <i>Fuel : the science and technology of fuel and energy </i>. 2026;424. doi:<a href=\"https://doi.org/10.1016/j.fuel.2026.139339\">10.1016/j.fuel.2026.139339</a>","chicago":"Türck, Julian, Carsten Funke, Fabian Schmitt, Jan Schneider, Hans-Jürgen Danneel, Ralf Türck, Wolfgang Ruck, and Jürgen Krahl. “Kinetic Study and Confirmation of Epoxide-Dependent Oligomerization of Methyl Oleate.” <i>Fuel : The Science and Technology of Fuel and Energy </i> 424 (2026). <a href=\"https://doi.org/10.1016/j.fuel.2026.139339\">https://doi.org/10.1016/j.fuel.2026.139339</a>."},"place":"Amsterdam [u.a.]","volume":424,"publication_status":"published"},{"publication_identifier":{"eissn":["1873-7072"],"issn":["0308-8146"]},"title":"Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities","publication":"Food Chemistry","status":"public","language":[{"iso":"eng"}],"user_id":"83781","place":"New York, NY [u.a.]","citation":{"ama":"Güneş ZS, Gülseren İ, Funke C, Danneel HJ, Esatbeyoglu T. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. <i>Food Chemistry</i>. 2026;528. doi:<a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">10.1016/j.foodchem.2026.150976</a>","chicago":"Güneş, Zeynep Saliha, İbrahim Gülseren, Carsten Funke, Hans-Jürgen Danneel, and Tuba Esatbeyoglu. “Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities.” <i>Food Chemistry</i> 528 (2026). <a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">https://doi.org/10.1016/j.foodchem.2026.150976</a>.","apa":"Güneş, Z. S., Gülseren, İ., Funke, C., Danneel, H.-J., &#38; Esatbeyoglu, T. (2026). Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. <i>Food Chemistry</i>, <i>528</i>, Article 150976. <a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">https://doi.org/10.1016/j.foodchem.2026.150976</a>","chicago-de":"Güneş, Zeynep Saliha, İbrahim Gülseren, Carsten Funke, Hans-Jürgen Danneel und Tuba Esatbeyoglu. 2026. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. <i>Food Chemistry</i> 528. doi:<a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">10.1016/j.foodchem.2026.150976</a>, .","mla":"Güneş, Zeynep Saliha, et al. “Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities.” <i>Food Chemistry</i>, vol. 528, 150976, 2026, <a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">https://doi.org/10.1016/j.foodchem.2026.150976</a>.","havard":"Z.S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, T. Esatbeyoglu, Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities, Food Chemistry. 528 (2026).","short":"Z.S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, T. Esatbeyoglu, Food Chemistry 528 (2026).","ieee":"Z. S. Güneş, İ. Gülseren, C. Funke, H.-J. Danneel, and T. Esatbeyoglu, “Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities,” <i>Food Chemistry</i>, vol. 528, Art. no. 150976, 2026, doi: <a href=\"https://doi.org/10.1016/j.foodchem.2026.150976\">10.1016/j.foodchem.2026.150976</a>.","van":"Güneş ZS, Gülseren İ, Funke C, Danneel HJ, Esatbeyoglu T. Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. Food Chemistry. 2026;528.","ufg":"<b>Güneş, Zeynep Saliha u. a.</b>: Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities, in: <i>Food Chemistry</i> 528 (2026).","din1505-2-1":"<span style=\"font-variant:small-caps;\">Güneş, Zeynep Saliha</span> ; <span style=\"font-variant:small-caps;\">Gülseren, İbrahim</span> ; <span style=\"font-variant:small-caps;\">Funke, Carsten</span> ; <span style=\"font-variant:small-caps;\">Danneel, Hans-Jürgen</span> ; <span style=\"font-variant:small-caps;\">Esatbeyoglu, Tuba</span>: Fractionation of sunflower protein hydrolysates by low pressure preparative gradient chromatography: An integrated in vitro and in silico assessment of bioactivities. In: <i>Food Chemistry</i> Bd. 528. New York, NY [u.a.], Elsevier BV (2026)","bjps":"<b>Güneş ZS <i>et al.</i></b> (2026) Fractionation of Sunflower Protein Hydrolysates by Low Pressure Preparative Gradient Chromatography: An Integrated in Vitro and in Silico Assessment of Bioactivities. <i>Food Chemistry</i> <b>528</b>."},"volume":528,"publication_status":"published","intvolume":"       528","keyword":["Sunflower protein hydrolysates","Fractionation","Bioactive peptides","ACE inhibition","DPP-IV inhibition","Mass spectrometry","In silico analysis"],"quality_controlled":"1","department":[{"_id":"DEP4018"}],"_id":"14057","year":"2026","date_created":"2026-09-24T07:39:35Z","type":"scientific_journal_article","article_number":"150976","author":[{"full_name":"Güneş, Zeynep Saliha","first_name":"Zeynep Saliha","last_name":"Güneş"},{"first_name":"İbrahim","last_name":"Gülseren","full_name":"Gülseren, İbrahim"},{"id":"74167","last_name":"Funke","first_name":"Carsten","full_name":"Funke, Carsten"},{"id":"12231","first_name":"Hans-Jürgen","last_name":"Danneel","full_name":"Danneel, Hans-Jürgen"},{"first_name":"Tuba","last_name":"Esatbeyoglu","full_name":"Esatbeyoglu, Tuba"}],"abstract":[{"lang":"eng","text":"Bioactive peptides from plant proteins offer significant benefits. In this study, the structural and bioactive properties of sunflower protein isolate and its enzymatic hydrolysates were systematically investigated. The hydrolysates were fractionated using low-pressure preparative gradient chromatography (LP-PGC), and their antioxidant capacity, along with angiotensin-converting enzyme and dipeptidyl peptidase-IV inhibitory activities relevant to metabolic health, were assessed. Structural analyses via sodium dodecyl sulfate polyacrylamide gel electrophoresis and scanning electron microscopy revealed significant alterations in molecular weight distribution and surface morphology after hydrolysis and fractionation, confirming the generation of low-molecular-weight peptides linked to improved bioactivity. The results demonstrated a clear fraction-dependent bioactivity profile. MTT-based cell viability analysis of HepG2 and Caco-2 cells indicates that the non-toxicity of the hydrolysates depends on both their concentration and the cell type. Molecular docking studies were performed with the three highest-scoring peptides from each hydrolysate according to PeptideRanker. LP-PGC effectively reveals in vitro/in silico bioactivity of sunflower protein hydrolysates for multifunctional food use."}],"publisher":"Elsevier BV","doi":"10.1016/j.foodchem.2026.150976","date_updated":"2026-09-25T09:08:20Z"},{"conference":{"location":"Online","name":"Fokustag where food meets IT","start_date":"02.03.2021","end_date":"02.03.2021"},"year":"2021","_id":"6842","language":[{"iso":"eng"}],"title":"Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik","status":"public","department":[{"_id":"DEP4000"},{"_id":"DEP4027"},{"_id":"DEP1308"},{"_id":"DEP4028"}],"date_updated":"2025-01-30T15:45:45Z","user_id":"83781","date_created":"2021-12-03T13:38:13Z","type":"conference","author":[{"orcid":" 0000-0003-0860-4696 ","id":"75850","first_name":"Daniel","last_name":"Pauli","full_name":"Pauli, Daniel"},{"full_name":"Neumaier, Michael","first_name":"Michael","last_name":"Neumaier","id":"77059"},{"full_name":"Scharf, Matthias","last_name":"Scharf","first_name":"Matthias","id":"65179"},{"full_name":"Funke, Carsten","last_name":"Funke","first_name":"Carsten","id":"74167"}],"citation":{"short":"D. Pauli, M. Neumaier, M. Scharf, C. Funke, in: 2021.","havard":"D. Pauli, M. Neumaier, M. Scharf, C. Funke, Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik, in: 2021.","van":"Pauli D, Neumaier M, Scharf M, Funke C. Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik. In 2021.","ieee":"D. Pauli, M. Neumaier, M. Scharf, and C. Funke, “Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik,” presented at the Fokustag where food meets IT, Online, 2021.","ufg":"<b>Pauli, Daniel u. a.</b>: Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik, in: o. Hg.: o. O. 2021.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Pauli, Daniel</span> ; <span style=\"font-variant:small-caps;\">Neumaier, Michael</span> ; <span style=\"font-variant:small-caps;\">Scharf, Matthias</span> ; <span style=\"font-variant:small-caps;\">Funke, Carsten</span>: Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik. In: , 2021","bjps":"<b>Pauli D <i>et al.</i></b> (2021) Von Der Offline Zur Online Qualitätskontrolle Mittels Echtzeit- Und Fingerprint-Analytik.","apa":"Pauli, D., Neumaier, M., Scharf, M., &#38; Funke, C. (2021). <i>Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik</i>. Fokustag where food meets IT, Online.","chicago-de":"Pauli, Daniel, Michael Neumaier, Matthias Scharf und Carsten Funke. 2021. Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik. In: .","mla":"Pauli, Daniel, et al. <i>Von Der Offline Zur Online Qualitätskontrolle Mittels Echtzeit- Und Fingerprint-Analytik</i>. 2021.","ama":"Pauli D, Neumaier M, Scharf M, Funke C. Von der offline zur online Qualitätskontrolle mittels Echtzeit- und Fingerprint-Analytik. In: ; 2021.","chicago":"Pauli, Daniel, Michael Neumaier, Matthias Scharf, and Carsten Funke. “Von Der Offline Zur Online Qualitätskontrolle Mittels Echtzeit- Und Fingerprint-Analytik,” 2021."}}]