---
_id: '13314'
abstract:
- lang: ger
  text: 'Pilze prägen die Lebensmittelindustrie: als wertvolle Helfer bei der Herstellung
    traditioneller und innovativer Produkte. Ebenso sind sie als Verursacher von Verderb
    und Mykotoxin-Bildung gefürchtet. Dieses Fachbuch bietet einen umfassenden Überblick
    über die Rolle von Schimmelpilzen und Hefen in allen Nahrungsmitteln.'
author:
- first_name: Frank
  full_name: Ebel, Frank
  last_name: Ebel
- first_name: Martin
  full_name: Weidenbörner, Martin
  last_name: Weidenbörner
citation:
  ama: 'Ebel F, Weidenbörner M. <i>Lebensmittel-Mykologie : Verderbsprozesse - Fermentation
    - biologische Hintergründe </i>. 2nd ed. (Ulrich S, ed.). Behr´s Verlag; 2026.'
  apa: 'Ebel, F., &#38; Weidenbörner, M. (2026). <i>Lebensmittel-Mykologie : Verderbsprozesse
    - Fermentation - biologische Hintergründe </i> (S. Ulrich, Ed.; 2nd ed.). Behr´s
    Verlag.'
  bjps: '<b>Ebel F and Weidenbörner M</b> (2026) <i>Lebensmittel-Mykologie : Verderbsprozesse
    - Fermentation - biologische Hintergründe </i>, 2nd ed., Ulrich S (ed.). Hamburg:
    Behr´s Verlag.'
  chicago: 'Ebel, Frank, and Martin Weidenbörner. <i>Lebensmittel-Mykologie : Verderbsprozesse
    - Fermentation - biologische Hintergründe </i>. Edited by Sebastian Ulrich. 2nd
    ed. Hamburg: Behr´s Verlag, 2026.'
  chicago-de: 'Ebel, Frank und Martin Weidenbörner. 2026. <i>Lebensmittel-Mykologie :
    Verderbsprozesse - Fermentation - biologische Hintergründe </i>. Hg. von Sebastian
    Ulrich. 2. Aufl. Hamburg: Behr´s Verlag.'
  din1505-2-1: '<span style="font-variant:small-caps;">Ebel, Frank</span> ; <span
    style="font-variant:small-caps;">Weidenbörner, Martin</span> ; <span style="font-variant:small-caps;">Ulrich,
    S.</span> (Hrsg.): <i>Lebensmittel-Mykologie : Verderbsprozesse - Fermentation
    - biologische Hintergründe </i>. 2. Aufl. Hamburg : Behr´s Verlag, 2026'
  havard: 'F. Ebel, M. Weidenbörner, Lebensmittel-Mykologie : Verderbsprozesse - Fermentation
    - biologische Hintergründe , 2nd ed., Behr´s Verlag, Hamburg, 2026.'
  ieee: 'F. Ebel and M. Weidenbörner, <i>Lebensmittel-Mykologie : Verderbsprozesse
    - Fermentation - biologische Hintergründe </i>, 2nd ed. Hamburg: Behr´s Verlag,
    2026.'
  mla: 'Ebel, Frank, and Martin Weidenbörner. <i>Lebensmittel-Mykologie : Verderbsprozesse
    - Fermentation - biologische Hintergründe </i>. Edited by Sebastian Ulrich, 2nd
    ed., Behr´s Verlag, 2026.'
  short: 'F. Ebel, M. Weidenbörner, Lebensmittel-Mykologie : Verderbsprozesse - Fermentation
    - biologische Hintergründe , 2nd ed., Behr´s Verlag, Hamburg, 2026.'
  ufg: '<b>Ebel, Frank/Weidenbörner, Martin</b>: Lebensmittel-Mykologie : Verderbsprozesse
    - Fermentation - biologische Hintergründe , hg. von Ulrich, Sebastian, Hamburg
    <sup>2</sup>2026.'
  van: 'Ebel F, Weidenbörner M. Lebensmittel-Mykologie : Verderbsprozesse - Fermentation
    - biologische Hintergründe . 2nd ed. Ulrich S, editor. Hamburg: Behr´s Verlag;
    2026. 452 p.'
date_created: 2025-12-02T10:36:29Z
date_updated: 2025-12-08T09:17:51Z
department:
- _id: DEP4010
edition: '2'
editor:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
language:
- iso: ger
page: '452'
place: Hamburg
publication_identifier:
  isbn:
  - 978-3-98892-026-3
publication_status: published
publisher: Behr´s Verlag
status: public
title: 'Lebensmittel-Mykologie : Verderbsprozesse - Fermentation - biologische Hintergründe '
type: book
user_id: '42121'
year: '2026'
...
---
_id: '12981'
abstract:
- lang: eng
  text: The aim of the study was to develop an alternative husbandry system for Japanese
    quail in a step-by-step procedure. The effects of various floor and nest variants
    on animal health, eggshell contamination and performance in three housing systems
    (alternative housing system, AHS; conventional cage system, CCS; floor housing
    system, FHS) were tested in altogether four laying periods, two of which are described
    in more detail. The analysis revealed that the plastic grid floor in AHS achieved
    the poorest results for toe and foot pad hyperkeratosis and foot pad dermatitis.
    Heavier quail showed more toe pad hyperkeratosis. The claw length was longest
    in CCS and shortest in FHS. Plumage damage on the head developed sooner and more
    often in CCS. Heavier quail showed significantly less plumage damage on the head.
    In general, the plumage condition of quail was better in FHS than in CCS and AHS.
    For eggshell hygiene, a higher microbial load was found on eggs from FHS, and
    there were no clear differences between CCS and AHS. The number of second-choice
    eggs was smallest in CCS and highest in FHS. Particularly the high soiling of
    eggs that were laid in the litter in FHS stood out. In summary, environmental
    enrichment had a positive effect on animal health, and FHS achieved the best results.
    Concerning eggshell hygiene and economic efficiency, the situation is the opposite.
    A floor material in AHS that suits both foot health and eggshell hygiene was not
    found, suggesting a need for further research.
article_number: '100004'
author:
- first_name: Lisa
  full_name: Wolf, Lisa
  last_name: Wolf
- first_name: Marie-Anna
  full_name: Hohenfeld, Marie-Anna
  last_name: Hohenfeld
- first_name: Lucas
  full_name: Rathmann, Lucas
  last_name: Rathmann
- first_name: Larissa
  full_name: Weinmann, Larissa
  last_name: Weinmann
- first_name: Ruben
  full_name: Schreiter, Ruben
  last_name: Schreiter
- first_name: Paul
  full_name: Schmidt, Paul
  last_name: Schmidt
- first_name: Stephanie
  full_name: Hiereth, Stephanie
  last_name: Hiereth
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Reinhard
  full_name: Straubinger, Reinhard
  last_name: Straubinger
- first_name: Klaus
  full_name: Damme, Klaus
  last_name: Damme
- first_name: Michael
  full_name: Erhard, Michael
  last_name: Erhard
- first_name: Angela
  full_name: Schwarzer, Angela
  last_name: Schwarzer
- first_name: Philipp
  full_name: Hofmann, Philipp
  last_name: Hofmann
- first_name: Helen
  full_name: Louton, Helen
  last_name: Louton
- first_name: Shana
  full_name: Bergmann, Shana
  last_name: Bergmann
citation:
  ama: 'Wolf L, Hohenfeld MA, Rathmann L, et al. Impact of various husbandry environments
    on animal health, eggshell hygiene and performance of Japanese quail layers (Coturnix
    japonica domestica). <i>European poultry science : EPS = Archiv für Geflügelkunde</i>.
    2025;89(1-2). doi:<a href="https://doi.org/10.1016/j.eups.2025.100004">10.1016/j.eups.2025.100004</a>'
  apa: 'Wolf, L., Hohenfeld, M.-A., Rathmann, L., Weinmann, L., Schreiter, R., Schmidt,
    P., Hiereth, S., Ulrich, S., Straubinger, R., Damme, K., Erhard, M., Schwarzer,
    A., Hofmann, P., Louton, H., &#38; Bergmann, S. (2025). Impact of various husbandry
    environments on animal health, eggshell hygiene and performance of Japanese quail
    layers (Coturnix japonica domestica). <i>European Poultry Science : EPS = Archiv
    Für Geflügelkunde</i>, <i>89</i>(1–2), Article 100004. <a href="https://doi.org/10.1016/j.eups.2025.100004">https://doi.org/10.1016/j.eups.2025.100004</a>'
  bjps: '<b>Wolf L <i>et al.</i></b> (2025) Impact of Various Husbandry Environments
    on Animal Health, Eggshell Hygiene and Performance of Japanese Quail Layers (Coturnix
    Japonica Domestica). <i>European poultry science : EPS = Archiv für Geflügelkunde</i>
    <b>89</b>.'
  chicago: 'Wolf, Lisa, Marie-Anna Hohenfeld, Lucas Rathmann, Larissa Weinmann, Ruben
    Schreiter, Paul Schmidt, Stephanie Hiereth, et al. “Impact of Various Husbandry
    Environments on Animal Health, Eggshell Hygiene and Performance of Japanese Quail
    Layers (Coturnix Japonica Domestica).” <i>European Poultry Science : EPS = Archiv
    Für Geflügelkunde</i> 89, no. 1–2 (2025). <a href="https://doi.org/10.1016/j.eups.2025.100004">https://doi.org/10.1016/j.eups.2025.100004</a>.'
  chicago-de: 'Wolf, Lisa, Marie-Anna Hohenfeld, Lucas Rathmann, Larissa Weinmann,
    Ruben Schreiter, Paul Schmidt, Stephanie Hiereth, u. a. 2025. Impact of various
    husbandry environments on animal health, eggshell hygiene and performance of Japanese
    quail layers (Coturnix japonica domestica). <i>European poultry science : EPS
    = Archiv für Geflügelkunde</i> 89, Nr. 1–2. doi:<a href="https://doi.org/10.1016/j.eups.2025.100004">10.1016/j.eups.2025.100004</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Wolf,
    Lisa</span> ; <span style="font-variant:small-caps;">Hohenfeld, Marie-Anna</span>
    ; <span style="font-variant:small-caps;">Rathmann, Lucas</span> ; <span style="font-variant:small-caps;">Weinmann,
    Larissa</span> ; <span style="font-variant:small-caps;">Schreiter, Ruben</span>
    ; <span style="font-variant:small-caps;">Schmidt, Paul</span> ; <span style="font-variant:small-caps;">Hiereth,
    Stephanie</span> ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>
    ; u. a.</span>: Impact of various husbandry environments on animal health, eggshell
    hygiene and performance of Japanese quail layers (Coturnix japonica domestica).
    In: <i>European poultry science : EPS = Archiv für Geflügelkunde</i> Bd. 89. Stuttgart,
    Ulmer (2025), Nr. 1–2'
  havard: 'L. Wolf, M.-A. Hohenfeld, L. Rathmann, L. Weinmann, R. Schreiter, P. Schmidt,
    S. Hiereth, S. Ulrich, R. Straubinger, K. Damme, M. Erhard, A. Schwarzer, P. Hofmann,
    H. Louton, S. Bergmann, Impact of various husbandry environments on animal health,
    eggshell hygiene and performance of Japanese quail layers (Coturnix japonica domestica),
    European Poultry Science : EPS = Archiv Für Geflügelkunde. 89 (2025).'
  ieee: 'L. Wolf <i>et al.</i>, “Impact of various husbandry environments on animal
    health, eggshell hygiene and performance of Japanese quail layers (Coturnix japonica
    domestica),” <i>European poultry science : EPS = Archiv für Geflügelkunde</i>,
    vol. 89, no. 1–2, Art. no. 100004, 2025, doi: <a href="https://doi.org/10.1016/j.eups.2025.100004">10.1016/j.eups.2025.100004</a>.'
  mla: 'Wolf, Lisa, et al. “Impact of Various Husbandry Environments on Animal Health,
    Eggshell Hygiene and Performance of Japanese Quail Layers (Coturnix Japonica Domestica).”
    <i>European Poultry Science : EPS = Archiv Für Geflügelkunde</i>, vol. 89, no.
    1–2, 100004, 2025, <a href="https://doi.org/10.1016/j.eups.2025.100004">https://doi.org/10.1016/j.eups.2025.100004</a>.'
  short: 'L. Wolf, M.-A. Hohenfeld, L. Rathmann, L. Weinmann, R. Schreiter, P. Schmidt,
    S. Hiereth, S. Ulrich, R. Straubinger, K. Damme, M. Erhard, A. Schwarzer, P. Hofmann,
    H. Louton, S. Bergmann, European Poultry Science : EPS = Archiv Für Geflügelkunde
    89 (2025).'
  ufg: '<b>Wolf, Lisa u. a.</b>: Impact of various husbandry environments on animal
    health, eggshell hygiene and performance of Japanese quail layers (Coturnix japonica
    domestica), in: <i>European poultry science : EPS = Archiv für Geflügelkunde</i>
    89 (2025), H. 1–2.'
  van: 'Wolf L, Hohenfeld MA, Rathmann L, Weinmann L, Schreiter R, Schmidt P, et al.
    Impact of various husbandry environments on animal health, eggshell hygiene and
    performance of Japanese quail layers (Coturnix japonica domestica). European poultry
    science : EPS = Archiv für Geflügelkunde. 2025;89(1–2).'
date_created: 2025-06-15T10:50:02Z
date_updated: 2025-06-16T14:17:52Z
department:
- _id: DEP4010
doi: 10.1016/j.eups.2025.100004
intvolume: '        89'
issue: 1-2
keyword:
- Alternative housing
- Foot pad dermatitis
- Animal welfare
- Husbandry system
- Environmental enrichment
language:
- iso: eng
place: Stuttgart
publication: 'European poultry science : EPS = Archiv für Geflügelkunde'
publication_identifier:
  eissn:
  - '1612-9199 '
publication_status: published
publisher: Ulmer
quality_controlled: '1'
status: public
title: Impact of various husbandry environments on animal health, eggshell hygiene
  and performance of Japanese quail layers (Coturnix japonica domestica)
type: scientific_journal_article
user_id: '83781'
volume: 89
year: '2025'
...
---
_id: '13233'
abstract:
- lang: eng
  text: 'Atranones are secondary metabolites produced by Stachybotrys chartarum, a
    mold frequently found in water-damaged indoor environments. In contrast to the
    well-characterized and highly toxic macrocyclic trichothecenes, atranones have
    received relatively limited scientific attention. Approximately 60% of S. chartarum
    isolates from indoor environments produce atranones, while 40% form macrocyclic
    trichothecenes. No strain has been shown to produce both, indicating that the
    biosynthetic pathways for these two mycotoxin classes are mutually exclusive.
    Atranones are dolabellane-like diterpenoids synthesized from geranylgeranyl pyrophosphate
    through multiple enzymatic steps encoded by a specific core gene cluster. While
    the genetic structure of this cluster has been elucidated, its regulatory mechanisms
    remain poorly understood. Notably, although atranone-producing S. chartarum strains
    have been isolated from indoor settings, no study has yet confirmed the actual
    production of atranones in indoor environments, leaving the question of real-world
    exposure unresolved. Experimental studies in cell cultures and animal models indicate
    that atranones possess pro-inflammatory and cytotoxic properties, including the
    induction of apoptosis and cell cycle arrest. Atranone Q has demonstrated antitumor
    activity against osteosarcoma cells in vitro, and more recently identified derivatives
    such as stachatranone and stachybatranone have shown preliminary cardioprotective
    effects under ischemic conditions. However, these pharmacological effects remain
    exploratory and require further validation in in vivo models. Major knowledge
    gaps concern the environmental triggers for atranone biosynthesis, their regulation,
    actual presence in built environments, and potential health risks. These areas
    represent key priorities for future research. '
author:
- first_name: Mareike
  full_name: Dabisch-Ruthe, Mareike
  id: '66516'
  last_name: Dabisch-Ruthe
  orcid: https://orcid.org/0009-0008-7644-0826
- first_name: Jens
  full_name: Pfannebecker, Jens
  id: '45690'
  last_name: Pfannebecker
  orcid: 0009-0005-4133-5442
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Frank
  full_name: Ebel, Frank
  last_name: Ebel
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
citation:
  ama: Dabisch-Ruthe M, Pfannebecker J, Straubinger RK, Ebel F, Ulrich S. Atranone-an
    underestimated secondary metabolite? <i>Mycotoxin Research</i>. Published online
    2025. doi:<a href="https://doi.org/10.1007/s12550-025-00609-x">10.1007/s12550-025-00609-x</a>
  apa: Dabisch-Ruthe, M., Pfannebecker, J., Straubinger, R. K., Ebel, F., &#38; Ulrich,
    S. (2025). Atranone-an underestimated secondary metabolite? <i>Mycotoxin Research</i>.
    <a href="https://doi.org/10.1007/s12550-025-00609-x">https://doi.org/10.1007/s12550-025-00609-x</a>
  bjps: <b>Dabisch-Ruthe M <i>et al.</i></b> (2025) Atranone-an Underestimated Secondary
    Metabolite? <i>Mycotoxin Research</i>.
  chicago: Dabisch-Ruthe, Mareike, Jens Pfannebecker, Reinhard K. Straubinger, Frank
    Ebel, and Sebastian Ulrich. “Atranone-an Underestimated Secondary Metabolite?”
    <i>Mycotoxin Research</i>, 2025. <a href="https://doi.org/10.1007/s12550-025-00609-x">https://doi.org/10.1007/s12550-025-00609-x</a>.
  chicago-de: Dabisch-Ruthe, Mareike, Jens Pfannebecker, Reinhard K. Straubinger,
    Frank Ebel und Sebastian Ulrich. 2025. Atranone-an underestimated secondary metabolite?
    <i>Mycotoxin Research</i>. doi:<a href="https://doi.org/10.1007/s12550-025-00609-x">10.1007/s12550-025-00609-x</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Dabisch-Ruthe, Mareike</span>
    ; <span style="font-variant:small-caps;">Pfannebecker, Jens</span> ; <span style="font-variant:small-caps;">Straubinger,
    Reinhard K.</span> ; <span style="font-variant:small-caps;">Ebel, Frank</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>: Atranone-an
    underestimated secondary metabolite? In: <i>Mycotoxin Research</i>. Berlin ; Heidelberg,
    Springer (2025)'
  havard: M. Dabisch-Ruthe, J. Pfannebecker, R.K. Straubinger, F. Ebel, S. Ulrich,
    Atranone-an underestimated secondary metabolite?, Mycotoxin Research. (2025).
  ieee: 'M. Dabisch-Ruthe, J. Pfannebecker, R. K. Straubinger, F. Ebel, and S. Ulrich,
    “Atranone-an underestimated secondary metabolite?,” <i>Mycotoxin Research</i>,
    2025, doi: <a href="https://doi.org/10.1007/s12550-025-00609-x">10.1007/s12550-025-00609-x</a>.'
  mla: Dabisch-Ruthe, Mareike, et al. “Atranone-an Underestimated Secondary Metabolite?”
    <i>Mycotoxin Research</i>, 2025, <a href="https://doi.org/10.1007/s12550-025-00609-x">https://doi.org/10.1007/s12550-025-00609-x</a>.
  short: M. Dabisch-Ruthe, J. Pfannebecker, R.K. Straubinger, F. Ebel, S. Ulrich,
    Mycotoxin Research (2025).
  ufg: '<b>Dabisch-Ruthe, Mareike u. a.</b>: Atranone-an underestimated secondary
    metabolite?, in: <i>Mycotoxin Research</i> (2025).'
  van: Dabisch-Ruthe M, Pfannebecker J, Straubinger RK, Ebel F, Ulrich S. Atranone-an
    underestimated secondary metabolite? Mycotoxin Research. 2025;
date_created: 2025-10-01T09:20:35Z
date_updated: 2025-10-06T12:11:00Z
department:
- _id: DEP4010
doi: 10.1007/s12550-025-00609-x
keyword:
- Atranone
- Secondary metabolite
- Stachybotrys
- Stachatranone
- Stachybatranone
language:
- iso: eng
place: Berlin ; Heidelberg
publication: Mycotoxin Research
publication_identifier:
  eissn:
  - 1867-1632
  issn:
  - 0178-7888
publication_status: published
publisher: Springer
status: public
title: Atranone-an underestimated secondary metabolite?
type: scientific_journal_article
user_id: '83781'
year: '2025'
...
---
_id: '12940'
abstract:
- lang: eng
  text: There are limited data on Lyme borreliosis (LB), a tick-borne disease caused
    by the Borrelia burgdorferi sensu lato complex, in horses. Seropositivity is not
    necessarily associated with clinical disease. Data on seropositivity against Borrelia
    burgdorferi and Anaplasma phagocytophilum in German horses are sparse. Therefore,
    serum samples from horses (n = 123) suspected of having Lyme borreliosis and clinically
    healthy horses (n = 113) from the same stables were tested for specific antibodies
    against Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum. The samples
    were screened for antibodies against Borrelia burgdorferi (ELISA and an IgG line
    immunoblot assay). Furthermore, the samples were examined for antibodies against
    B. burgdorferi and Anaplasma phagocytophilum with a validated rapid in-house test
    (SNAP® 4Dx Plus® ELISA). The clinical signs of suspect horses included lameness
    (n = 36), poor performance (n = 19), and apathy (n = 12). Twenty-three percent
    (n = 26) of suspect horses and 17% (n = 18) of clinically healthy horses were
    seropositive for having a Borrelia burgdorferi sensu lato infection (p = 0.371),
    showing that the detection of specific antibodies against B. burgdorferi alone
    is not sufficient for a diagnosis of equine LB. Anaplasma phagocytophilum seropositivity
    and seropositivity against both pathogens was 20%/6% in suspect horses and 16%/2%
    in the clinically healthy population, showing only minor differences (p = 0.108).
    Unspecific testing for antibodies against B. burgdorferi without clinical suspicion
    of Lyme borreliosis is not recommended since the clinical relevance of seropositivity
    against Borrelia burgdorferi sensu lato remains to be elucidated.
article_number: '1984'
author:
- first_name: Heidrun
  full_name: Gehlen, Heidrun
  last_name: Gehlen
- first_name: Katharina
  full_name: Inerle, Katharina
  last_name: Inerle
- first_name: Alexander
  full_name: Bartel, Alexander
  last_name: Bartel
- first_name: Sabita Diana
  full_name: Stöckle, Sabita Diana
  last_name: Stöckle
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Beatrice
  full_name: Briese, Beatrice
  last_name: Briese
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: Gehlen H, Inerle K, Bartel A, et al. Seroprevalence of Borrelia burgdorferi
    sensu lato and Anaplasma phagocytophilum Infections in German Horses. <i>Animals</i>.
    2023;13(12). doi:<a href="https://doi.org/10.3390/ani13121984">10.3390/ani13121984</a>
  apa: Gehlen, H., Inerle, K., Bartel, A., Stöckle, S. D., Ulrich, S., Briese, B.,
    &#38; Straubinger, R. K. (2023). Seroprevalence of Borrelia burgdorferi sensu
    lato and Anaplasma phagocytophilum Infections in German Horses. <i>Animals</i>,
    <i>13</i>(12), Article 1984. <a href="https://doi.org/10.3390/ani13121984">https://doi.org/10.3390/ani13121984</a>
  bjps: <b>Gehlen H <i>et al.</i></b> (2023) Seroprevalence of Borrelia Burgdorferi
    Sensu Lato and Anaplasma Phagocytophilum Infections in German Horses. <i>Animals</i>
    <b>13</b>.
  chicago: Gehlen, Heidrun, Katharina Inerle, Alexander Bartel, Sabita Diana Stöckle,
    Sebastian Ulrich, Beatrice Briese, and Reinhard K. Straubinger. “Seroprevalence
    of Borrelia Burgdorferi Sensu Lato and Anaplasma Phagocytophilum Infections in
    German Horses.” <i>Animals</i> 13, no. 12 (2023). <a href="https://doi.org/10.3390/ani13121984">https://doi.org/10.3390/ani13121984</a>.
  chicago-de: Gehlen, Heidrun, Katharina Inerle, Alexander Bartel, Sabita Diana Stöckle,
    Sebastian Ulrich, Beatrice Briese und Reinhard K. Straubinger. 2023. Seroprevalence
    of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in
    German Horses. <i>Animals</i> 13, Nr. 12. doi:<a href="https://doi.org/10.3390/ani13121984">10.3390/ani13121984</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Gehlen, Heidrun</span> ; <span
    style="font-variant:small-caps;">Inerle, Katharina</span> ; <span style="font-variant:small-caps;">Bartel,
    Alexander</span> ; <span style="font-variant:small-caps;">Stöckle, Sabita Diana</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Briese,
    Beatrice</span> ; <span style="font-variant:small-caps;">Straubinger, Reinhard
    K.</span>: Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum
    Infections in German Horses. In: <i>Animals</i> Bd. 13. Basel, MDPI AG (2023),
    Nr. 12'
  havard: H. Gehlen, K. Inerle, A. Bartel, S.D. Stöckle, S. Ulrich, B. Briese, R.K.
    Straubinger, Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum
    Infections in German Horses, Animals. 13 (2023).
  ieee: 'H. Gehlen <i>et al.</i>, “Seroprevalence of Borrelia burgdorferi sensu lato
    and Anaplasma phagocytophilum Infections in German Horses,” <i>Animals</i>, vol.
    13, no. 12, Art. no. 1984, 2023, doi: <a href="https://doi.org/10.3390/ani13121984">10.3390/ani13121984</a>.'
  mla: Gehlen, Heidrun, et al. “Seroprevalence of Borrelia Burgdorferi Sensu Lato
    and Anaplasma Phagocytophilum Infections in German Horses.” <i>Animals</i>, vol.
    13, no. 12, 1984, 2023, <a href="https://doi.org/10.3390/ani13121984">https://doi.org/10.3390/ani13121984</a>.
  short: H. Gehlen, K. Inerle, A. Bartel, S.D. Stöckle, S. Ulrich, B. Briese, R.K.
    Straubinger, Animals 13 (2023).
  ufg: '<b>Gehlen, Heidrun u. a.</b>: Seroprevalence of Borrelia burgdorferi sensu
    lato and Anaplasma phagocytophilum Infections in German Horses, in: <i>Animals</i>
    13 (2023), H. 12.'
  van: Gehlen H, Inerle K, Bartel A, Stöckle SD, Ulrich S, Briese B, et al. Seroprevalence
    of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in
    German Horses. Animals. 2023;13(12).
date_created: 2025-06-15T09:35:35Z
date_updated: 2025-06-16T09:16:39Z
department:
- _id: DEP4010
doi: 10.3390/ani13121984
extern: '1'
intvolume: '        13'
issue: '12'
keyword:
- equine Lyme borreliosis
- equine granulocytic anaplasmosis
- seroprevalence
- co-infection
language:
- iso: eng
place: Basel
publication: Animals
publication_identifier:
  eissn:
  - 2076-2615
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum
  Infections in German Horses
type: scientific_journal_article
user_id: '83781'
volume: 13
year: '2023'
...
---
_id: '12941'
abstract:
- lang: eng
  text: Stachybotrys chartarum (Hypocreales, Ascomycota) is a toxigenic fungus that
    is frequently isolated from water-damaged buildings or improperly stored feed.
    The secondary metabolites formed by this mold have been associated with health
    problems in humans and animals. Several authors have studied the influence of
    environmental conditions on the production of mycotoxins, but these studies focused
    on undefined or complex substrates, such as building materials and media that
    impeded investigations of the influence of specific nutrients. In this study,
    a chemically defined cultivation medium was used to investigate the impact of
    several nitrogen and carbon sources on growth of S. chartarum and its production
    of macrocyclic trichothecenes (MTs) and stachybotrylactam (STLAC). Increasing
    concentrations of sodium nitrate were found to positively affect mycelial growth,
    the level of sporulation, and MT production, while ammonium nitrate and ammonium
    chloride had an inhibitory effect. Potato starch was the superior and most reliable
    carbon source tested. Additionally, we observed that the level of sporulation
    was correlated with the production of MTs but not with that of STLAC. In this
    study, we provide a chemically well-defined cultivation medium suitable for standardized
    in vitro testing of the capacity of S. chartarum isolates to produce macrocyclic
    trichothecenes.
author:
- first_name: Katharina
  full_name: Tribelhorn, Katharina
  last_name: Tribelhorn
- first_name: Magdalena
  full_name: Twarużek, Magdalena
  last_name: Twarużek
- first_name: Robert
  full_name: Kosicki, Robert
  last_name: Kosicki
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Frank
  full_name: Ebel, Frank
  last_name: Ebel
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
citation:
  ama: Tribelhorn K, Twarużek M, Kosicki R, Straubinger RK, Ebel F, Ulrich S. A Chemically
    Defined Medium That Supports Mycotoxin Production by Stachybotrys chartarum Enabled
    Analysis of the Impact of Nitrogen and Carbon Sources on the Biosynthesis of Macrocyclic
    Trichothecenes and Stachybotrylactam. <i>Applied and Environmental Microbiology</i>.
    2023;89(7). doi:<a href="https://doi.org/10.1128/aem.00163-23">10.1128/aem.00163-23</a>
  apa: Tribelhorn, K., Twarużek, M., Kosicki, R., Straubinger, R. K., Ebel, F., &#38;
    Ulrich, S. (2023). A Chemically Defined Medium That Supports Mycotoxin Production
    by Stachybotrys chartarum Enabled Analysis of the Impact of Nitrogen and Carbon
    Sources on the Biosynthesis of Macrocyclic Trichothecenes and Stachybotrylactam.
    <i>Applied and Environmental Microbiology</i>, <i>89</i>(7). <a href="https://doi.org/10.1128/aem.00163-23">https://doi.org/10.1128/aem.00163-23</a>
  bjps: <b>Tribelhorn K <i>et al.</i></b> (2023) A Chemically Defined Medium That
    Supports Mycotoxin Production by Stachybotrys Chartarum Enabled Analysis of the
    Impact of Nitrogen and Carbon Sources on the Biosynthesis of Macrocyclic Trichothecenes
    and Stachybotrylactam. <i>Applied and Environmental Microbiology</i> <b>89</b>.
  chicago: Tribelhorn, Katharina, Magdalena Twarużek, Robert Kosicki, Reinhard K.
    Straubinger, Frank Ebel, and Sebastian Ulrich. “A Chemically Defined Medium That
    Supports Mycotoxin Production by Stachybotrys Chartarum Enabled Analysis of the
    Impact of Nitrogen and Carbon Sources on the Biosynthesis of Macrocyclic Trichothecenes
    and Stachybotrylactam.” <i>Applied and Environmental Microbiology</i> 89, no.
    7 (2023). <a href="https://doi.org/10.1128/aem.00163-23">https://doi.org/10.1128/aem.00163-23</a>.
  chicago-de: Tribelhorn, Katharina, Magdalena Twarużek, Robert Kosicki, Reinhard
    K. Straubinger, Frank Ebel und Sebastian Ulrich. 2023. A Chemically Defined Medium
    That Supports Mycotoxin Production by Stachybotrys chartarum Enabled Analysis
    of the Impact of Nitrogen and Carbon Sources on the Biosynthesis of Macrocyclic
    Trichothecenes and Stachybotrylactam. <i>Applied and Environmental Microbiology</i>
    89, Nr. 7. doi:<a href="https://doi.org/10.1128/aem.00163-23">10.1128/aem.00163-23</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Tribelhorn, Katharina</span>
    ; <span style="font-variant:small-caps;">Twarużek, Magdalena</span> ; <span style="font-variant:small-caps;">Kosicki,
    Robert</span> ; <span style="font-variant:small-caps;">Straubinger, Reinhard K.</span>
    ; <span style="font-variant:small-caps;">Ebel, Frank</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span>: A Chemically Defined Medium That Supports Mycotoxin Production
    by Stachybotrys chartarum Enabled Analysis of the Impact of Nitrogen and Carbon
    Sources on the Biosynthesis of Macrocyclic Trichothecenes and Stachybotrylactam.
    In: <i>Applied and Environmental Microbiology</i> Bd. 89.   Washington, DC [u.a.],
    American Society for Microbiology (2023), Nr. 7'
  havard: K. Tribelhorn, M. Twarużek, R. Kosicki, R.K. Straubinger, F. Ebel, S. Ulrich,
    A Chemically Defined Medium That Supports Mycotoxin Production by Stachybotrys
    chartarum Enabled Analysis of the Impact of Nitrogen and Carbon Sources on the
    Biosynthesis of Macrocyclic Trichothecenes and Stachybotrylactam, Applied and
    Environmental Microbiology. 89 (2023).
  ieee: 'K. Tribelhorn, M. Twarużek, R. Kosicki, R. K. Straubinger, F. Ebel, and S.
    Ulrich, “A Chemically Defined Medium That Supports Mycotoxin Production by Stachybotrys
    chartarum Enabled Analysis of the Impact of Nitrogen and Carbon Sources on the
    Biosynthesis of Macrocyclic Trichothecenes and Stachybotrylactam,” <i>Applied
    and Environmental Microbiology</i>, vol. 89, no. 7, 2023, doi: <a href="https://doi.org/10.1128/aem.00163-23">10.1128/aem.00163-23</a>.'
  mla: Tribelhorn, Katharina, et al. “A Chemically Defined Medium That Supports Mycotoxin
    Production by Stachybotrys Chartarum Enabled Analysis of the Impact of Nitrogen
    and Carbon Sources on the Biosynthesis of Macrocyclic Trichothecenes and Stachybotrylactam.”
    <i>Applied and Environmental Microbiology</i>, vol. 89, no. 7, 2023, <a href="https://doi.org/10.1128/aem.00163-23">https://doi.org/10.1128/aem.00163-23</a>.
  short: K. Tribelhorn, M. Twarużek, R. Kosicki, R.K. Straubinger, F. Ebel, S. Ulrich,
    Applied and Environmental Microbiology 89 (2023).
  ufg: '<b>Tribelhorn, Katharina u. a.</b>: A Chemically Defined Medium That Supports
    Mycotoxin Production by Stachybotrys chartarum Enabled Analysis of the Impact
    of Nitrogen and Carbon Sources on the Biosynthesis of Macrocyclic Trichothecenes
    and Stachybotrylactam, in: <i>Applied and Environmental Microbiology</i> 89 (2023),
    H. 7.'
  van: Tribelhorn K, Twarużek M, Kosicki R, Straubinger RK, Ebel F, Ulrich S. A Chemically
    Defined Medium That Supports Mycotoxin Production by Stachybotrys chartarum Enabled
    Analysis of the Impact of Nitrogen and Carbon Sources on the Biosynthesis of Macrocyclic
    Trichothecenes and Stachybotrylactam. Applied and Environmental Microbiology.
    2023;89(7).
date_created: 2025-06-15T09:51:52Z
date_updated: 2025-06-16T09:21:50Z
department:
- _id: DEP4010
doi: 10.1128/aem.00163-23
extern: '1'
intvolume: '        89'
issue: '7'
language:
- iso: eng
place: '  Washington, DC [u.a.]'
publication: Applied and Environmental Microbiology
publication_identifier:
  eissn:
  - 1098-5336
  issn:
  - 0099-2240
publication_status: published
publisher: American Society for Microbiology
quality_controlled: '1'
status: public
title: A Chemically Defined Medium That Supports Mycotoxin Production by Stachybotrys
  chartarum Enabled Analysis of the Impact of Nitrogen and Carbon Sources on the Biosynthesis
  of Macrocyclic Trichothecenes and Stachybotrylactam
type: scientific_journal_article
user_id: '83781'
volume: 89
year: '2023'
...
---
_id: '12942'
abstract:
- lang: eng
  text: The ectoparasite Ixodes ricinus is an important vector for many tick-borne
    diseases (TBD) in the northern hemisphere, such as Lyme borreliosis, rickettsiosis,
    human granulocytic anaplasmosis, or tick-borne encephalitis virus. As climate
    change will lead to rising temperatures in the next years, we expect an increase
    in tick activity, tick population, and thus in the spread of TBD. Consequently,
    it has never been more critical to understand relationships within the microbial
    communities in ticks that might contribute to the tick’s fitness and the occurrence
    of TBD. Therefore, we analyzed the microbiota in different tick tissues such as
    midgut, salivary glands, and residual tick material, as well as the microbiota
    in complete Ixodes ricinus ticks using 16S rRNA gene amplicon sequencing. By using
    a newly developed DNA extraction protocol for tick tissue samples and a self-designed
    mock community, we were able to detect endosymbionts and pathogens that have been
    described in the literature previously. Further, this study displayed the usefulness
    of including a mock community during bioinformatic analysis to identify essential
    bacteria within the tick.
article_number: '1100'
author:
- first_name: Anna
  full_name: Wiesinger, Anna
  last_name: Wiesinger
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Stephanie
  full_name: Hiereth, Stephanie
  last_name: Hiereth
- first_name: Lidia
  full_name: Chitimia-Dobler, Lidia
  last_name: Chitimia-Dobler
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: 'Wiesinger A, Wenderlein J, Ulrich S, Hiereth S, Chitimia-Dobler L, Straubinger
    RK. Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota
    of Female Ixodes ricinus Ticks. <i>International Journal of Molecular Sciences</i>.
    2023;24(2). doi:<a href="https://doi.org/10.3390/ijms24021100">10.3390/ijms24021100</a>'
  apa: 'Wiesinger, A., Wenderlein, J., Ulrich, S., Hiereth, S., Chitimia-Dobler, L.,
    &#38; Straubinger, R. K. (2023). Revealing the Tick Microbiome: Insights into
    Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks. <i>International
    Journal of Molecular Sciences</i>, <i>24</i>(2), Article 1100. <a href="https://doi.org/10.3390/ijms24021100">https://doi.org/10.3390/ijms24021100</a>'
  bjps: '<b>Wiesinger A <i>et al.</i></b> (2023) Revealing the Tick Microbiome: Insights
    into Midgut and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks. <i>International
    Journal of Molecular Sciences</i> <b>24</b>.'
  chicago: 'Wiesinger, Anna, Jasmin Wenderlein, Sebastian Ulrich, Stephanie Hiereth,
    Lidia Chitimia-Dobler, and Reinhard K. Straubinger. “Revealing the Tick Microbiome:
    Insights into Midgut and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks.”
    <i>International Journal of Molecular Sciences</i> 24, no. 2 (2023). <a href="https://doi.org/10.3390/ijms24021100">https://doi.org/10.3390/ijms24021100</a>.'
  chicago-de: 'Wiesinger, Anna, Jasmin Wenderlein, Sebastian Ulrich, Stephanie Hiereth,
    Lidia Chitimia-Dobler und Reinhard K. Straubinger. 2023. Revealing the Tick Microbiome:
    Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks.
    <i>International Journal of Molecular Sciences</i> 24, Nr. 2. doi:<a href="https://doi.org/10.3390/ijms24021100">10.3390/ijms24021100</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Wiesinger, Anna</span> ; <span
    style="font-variant:small-caps;">Wenderlein, Jasmin</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Hiereth, Stephanie</span>
    ; <span style="font-variant:small-caps;">Chitimia-Dobler, Lidia</span> ; <span
    style="font-variant:small-caps;">Straubinger, Reinhard K.</span>: Revealing the
    Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female
    Ixodes ricinus Ticks. In: <i>International Journal of Molecular Sciences</i> Bd.
    24. Basel, MDPI AG (2023), Nr. 2'
  havard: 'A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler,
    R.K. Straubinger, Revealing the Tick Microbiome: Insights into Midgut and Salivary
    Gland Microbiota of Female Ixodes ricinus Ticks, International Journal of Molecular
    Sciences. 24 (2023).'
  ieee: 'A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler, and
    R. K. Straubinger, “Revealing the Tick Microbiome: Insights into Midgut and Salivary
    Gland Microbiota of Female Ixodes ricinus Ticks,” <i>International Journal of
    Molecular Sciences</i>, vol. 24, no. 2, Art. no. 1100, 2023, doi: <a href="https://doi.org/10.3390/ijms24021100">10.3390/ijms24021100</a>.'
  mla: 'Wiesinger, Anna, et al. “Revealing the Tick Microbiome: Insights into Midgut
    and Salivary Gland Microbiota of Female Ixodes Ricinus Ticks.” <i>International
    Journal of Molecular Sciences</i>, vol. 24, no. 2, 1100, 2023, <a href="https://doi.org/10.3390/ijms24021100">https://doi.org/10.3390/ijms24021100</a>.'
  short: A. Wiesinger, J. Wenderlein, S. Ulrich, S. Hiereth, L. Chitimia-Dobler, R.K.
    Straubinger, International Journal of Molecular Sciences 24 (2023).
  ufg: '<b>Wiesinger, Anna u. a.</b>: Revealing the Tick Microbiome: Insights into
    Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks, in: <i>International
    Journal of Molecular Sciences</i> 24 (2023), H. 2.'
  van: 'Wiesinger A, Wenderlein J, Ulrich S, Hiereth S, Chitimia-Dobler L, Straubinger
    RK. Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota
    of Female Ixodes ricinus Ticks. International Journal of Molecular Sciences. 2023;24(2).'
date_created: 2025-06-15T09:53:01Z
date_updated: 2025-06-16T09:25:29Z
department:
- _id: DEP4010
doi: 10.3390/ijms24021100
extern: '1'
intvolume: '        24'
issue: '2'
keyword:
- Ixodes ricinus
- microbiome
- tick-borne disease
- salivary glands
- midgut
- endosymbiont
- Candidatus Midichloria mitochondrii
language:
- iso: eng
place: Basel
publication: International Journal of Molecular Sciences
publication_identifier:
  eissn:
  - 1422-0067
  issn:
  - 1661-6596
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: 'Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota
  of Female Ixodes ricinus Ticks'
type: scientific_journal_article
user_id: '83781'
volume: 24
year: '2023'
...
---
_id: '12943'
abstract:
- lang: eng
  text: Brown trout (Salmo trutta) is an important aquaculture species in Germany,
    but its production faces challenges due to global warming and a high embryo mortality.
    Climate factors might influence the fish’s bacterial community (BC) and thus increase
    embryo mortality. Yet, knowledge of the physiological BC during ontogeny in general
    is scarce. In this project, the BC of brown trout has been investigated in a period
    from unfertilized egg to 95 days post fertilization (dpf) using 16S rRNA gene
    amplicon sequencing. Developmental changes differed between early and late ontogeny
    and major differences in BC occurred especially during early developmental stages.
    Thus, analysis was conducted separately for 0 to 67 dpf and from 67 to 95 dpf.
    All analyzed stages were sampled in toto to avoid bias due to different sampling
    methods in different developmental stages. The most abundant phylum in the BC
    of all developmental stages was Pseudomonadota, while only two families (Comamonadaceae
    and Moraxellaceae) occurred in all developmental stages. The early developmental
    stages until 67 dpf displayed greater shifts in their BC regarding bacterial richness,
    microbial diversity, and taxonomic composition. Thereafter, in the fry stages,
    the BC seemed to stabilize and changes were moderate. In future studies, a reduction
    in the sampling time frames during early development, an increase in sampling
    numbers, and an attempt for biological reproduction in order to characterize the
    causes of these variations is recommended.
article_number: '211'
author:
- first_name: Katharina
  full_name: Keiz, Katharina
  last_name: Keiz
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Patrick
  full_name: Keferloher, Patrick
  last_name: Keferloher
- first_name: Anna
  full_name: Wiesinger, Anna
  last_name: Wiesinger
- first_name: Klaus
  full_name: Neuhaus, Klaus
  last_name: Neuhaus
- first_name: Ilias
  full_name: Lagkouvardos, Ilias
  last_name: Lagkouvardos
- first_name: Helmut
  full_name: Wedekind, Helmut
  last_name: Wedekind
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: 'Keiz K, Ulrich S, Wenderlein J, et al. The Development of the Bacterial Community
    of Brown Trout (Salmo trutta) during Ontogeny. <i>Microorganisms : open access
    journal </i>. 2023;11(1). doi:<a href="https://doi.org/10.3390/microorganisms11010211">10.3390/microorganisms11010211</a>'
  apa: 'Keiz, K., Ulrich, S., Wenderlein, J., Keferloher, P., Wiesinger, A., Neuhaus,
    K., Lagkouvardos, I., Wedekind, H., &#38; Straubinger, R. K. (2023). The Development
    of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny. <i>Microorganisms :
    Open Access Journal </i>, <i>11</i>(1), Article 211. <a href="https://doi.org/10.3390/microorganisms11010211">https://doi.org/10.3390/microorganisms11010211</a>'
  bjps: '<b>Keiz K <i>et al.</i></b> (2023) The Development of the Bacterial Community
    of Brown Trout (Salmo Trutta) during Ontogeny. <i>Microorganisms : open access
    journal </i> <b>11</b>.'
  chicago: 'Keiz, Katharina, Sebastian Ulrich, Jasmin Wenderlein, Patrick Keferloher,
    Anna Wiesinger, Klaus Neuhaus, Ilias Lagkouvardos, Helmut Wedekind, and Reinhard
    K. Straubinger. “The Development of the Bacterial Community of Brown Trout (Salmo
    Trutta) during Ontogeny.” <i>Microorganisms : Open Access Journal </i> 11, no.
    1 (2023). <a href="https://doi.org/10.3390/microorganisms11010211">https://doi.org/10.3390/microorganisms11010211</a>.'
  chicago-de: 'Keiz, Katharina, Sebastian Ulrich, Jasmin Wenderlein, Patrick Keferloher,
    Anna Wiesinger, Klaus Neuhaus, Ilias Lagkouvardos, Helmut Wedekind und Reinhard
    K. Straubinger. 2023. The Development of the Bacterial Community of Brown Trout
    (Salmo trutta) during Ontogeny. <i>Microorganisms : open access journal </i> 11,
    Nr. 1. doi:<a href="https://doi.org/10.3390/microorganisms11010211">10.3390/microorganisms11010211</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Keiz,
    Katharina</span> ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>
    ; <span style="font-variant:small-caps;">Wenderlein, Jasmin</span> ; <span style="font-variant:small-caps;">Keferloher,
    Patrick</span> ; <span style="font-variant:small-caps;">Wiesinger, Anna</span>
    ; <span style="font-variant:small-caps;">Neuhaus, Klaus</span> ; <span style="font-variant:small-caps;">Lagkouvardos,
    Ilias</span> ; <span style="font-variant:small-caps;">Wedekind, Helmut</span>
    ; u. a.</span>: The Development of the Bacterial Community of Brown Trout (Salmo
    trutta) during Ontogeny. In: <i>Microorganisms : open access journal </i> Bd.
    11. Basel, MDPI AG (2023), Nr. 1'
  havard: 'K. Keiz, S. Ulrich, J. Wenderlein, P. Keferloher, A. Wiesinger, K. Neuhaus,
    I. Lagkouvardos, H. Wedekind, R.K. Straubinger, The Development of the Bacterial
    Community of Brown Trout (Salmo trutta) during Ontogeny, Microorganisms : Open
    Access Journal . 11 (2023).'
  ieee: 'K. Keiz <i>et al.</i>, “The Development of the Bacterial Community of Brown
    Trout (Salmo trutta) during Ontogeny,” <i>Microorganisms : open access journal
    </i>, vol. 11, no. 1, Art. no. 211, 2023, doi: <a href="https://doi.org/10.3390/microorganisms11010211">10.3390/microorganisms11010211</a>.'
  mla: 'Keiz, Katharina, et al. “The Development of the Bacterial Community of Brown
    Trout (Salmo Trutta) during Ontogeny.” <i>Microorganisms : Open Access Journal
    </i>, vol. 11, no. 1, 211, 2023, <a href="https://doi.org/10.3390/microorganisms11010211">https://doi.org/10.3390/microorganisms11010211</a>.'
  short: 'K. Keiz, S. Ulrich, J. Wenderlein, P. Keferloher, A. Wiesinger, K. Neuhaus,
    I. Lagkouvardos, H. Wedekind, R.K. Straubinger, Microorganisms : Open Access Journal  11
    (2023).'
  ufg: '<b>Keiz, Katharina u. a.</b>: The Development of the Bacterial Community of
    Brown Trout (Salmo trutta) during Ontogeny, in: <i>Microorganisms : open access
    journal </i> 11 (2023), H. 1.'
  van: 'Keiz K, Ulrich S, Wenderlein J, Keferloher P, Wiesinger A, Neuhaus K, et al.
    The Development of the Bacterial Community of Brown Trout (Salmo trutta) during
    Ontogeny. Microorganisms : open access journal . 2023;11(1).'
date_created: 2025-06-15T09:53:31Z
date_updated: 2025-06-16T11:09:43Z
department:
- _id: DEP4010
doi: 10.3390/microorganisms11010211
extern: '1'
intvolume: '        11'
issue: '1'
keyword:
- fish
- brown trout
- Salmo trutta
- development
- ontogeny
- microbiome
- bacterial community
language:
- iso: eng
place: Basel
publication: 'Microorganisms : open access journal '
publication_identifier:
  eissn:
  - 2076-2607
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: The Development of the Bacterial Community of Brown Trout (Salmo trutta) during
  Ontogeny
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2023'
...
---
_id: '12944'
abstract:
- lang: eng
  text: Lyme borreliosis is a vector-borne disease in humans and animals caused by
    bacteria from the Borrelia burgdorferi sensu lato complex (Bbsl). The possible
    transmission of Bbsl from companion animals to humans via ticks makes this disease
    important in terms of One Health approaches. Thus, early and accurate diagnosis
    and treatment are of utmost importance. Today’s standard for the detection of
    specific antibodies against Bbsl is a two-tiered test system based on an ELISA
    for screening combined with a line immunoassay (LIA) for confirmation. In this
    study, 200 canine and 200 equine serum samples with known antibody status were
    tested with two different LIAs (A and B). Results were compared regarding sensitivity,
    specificity, the diagnostic outcome for dogs and horses, as well as operability
    of the test. The results for canine serum samples corresponded to 94.0%, making
    both LIAs a good choice for LB diagnostic in dogs. For equine serum samples, the
    agreement of both tests was 65.5%, displaying the challenge equine samples still
    provide in LB diagnostic. Major concerns were the interpretation of the OspA antigen
    (AG) signal and the use of unspecific (i.e., p100/p83) or too sensitive signals
    on the LIA. The operability of both LIAs was equally user-friendly. Regarding
    the tests’ evaluation, the scanning process provided by LIA A was a major advantage
    considering the comparability of the tests.
article_number: '633'
author:
- first_name: Sophie
  full_name: Doff, Sophie
  last_name: Doff
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Anna
  full_name: Wiesinger, Anna
  last_name: Wiesinger
- first_name: Stephanie
  full_name: Hiereth, Stephanie
  last_name: Hiereth
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Reinhard
  full_name: Straubinger, Reinhard
  last_name: Straubinger
citation:
  ama: 'Doff S, Wenderlein J, Wiesinger A, Hiereth S, Ulrich S, Straubinger R. Detection
    of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of Canine and Equine
    Origin—A Comparative Study with Two Line Immunoassays. <i>Veterinary Sciences :
    open access journal</i>. 2022;9(11). doi:<a href="https://doi.org/10.3390/vetsci9110633">10.3390/vetsci9110633</a>'
  apa: 'Doff, S., Wenderlein, J., Wiesinger, A., Hiereth, S., Ulrich, S., &#38; Straubinger,
    R. (2022). Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies in
    Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays.
    <i>Veterinary Sciences : Open Access Journal</i>, <i>9</i>(11), Article 633. <a
    href="https://doi.org/10.3390/vetsci9110633">https://doi.org/10.3390/vetsci9110633</a>'
  bjps: '<b>Doff S <i>et al.</i></b> (2022) Detection of Borrelia Burgdorferi Sensu-Lato-Specific
    Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line
    Immunoassays. <i>Veterinary Sciences : open access journal</i> <b>9</b>.'
  chicago: 'Doff, Sophie, Jasmin Wenderlein, Anna Wiesinger, Stephanie Hiereth, Sebastian
    Ulrich, and Reinhard Straubinger. “Detection of Borrelia Burgdorferi Sensu-Lato-Specific
    Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line
    Immunoassays.” <i>Veterinary Sciences : Open Access Journal</i> 9, no. 11 (2022).
    <a href="https://doi.org/10.3390/vetsci9110633">https://doi.org/10.3390/vetsci9110633</a>.'
  chicago-de: 'Doff, Sophie, Jasmin Wenderlein, Anna Wiesinger, Stephanie Hiereth,
    Sebastian Ulrich und Reinhard Straubinger. 2022. Detection of Borrelia burgdorferi
    sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative
    Study with Two Line Immunoassays. <i>Veterinary Sciences : open access journal</i>
    9, Nr. 11. doi:<a href="https://doi.org/10.3390/vetsci9110633">10.3390/vetsci9110633</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Doff, Sophie</span> ; <span
    style="font-variant:small-caps;">Wenderlein, Jasmin</span> ; <span style="font-variant:small-caps;">Wiesinger,
    Anna</span> ; <span style="font-variant:small-caps;">Hiereth, Stephanie</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Straubinger,
    Reinhard</span>: Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies
    in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays.
    In: <i>Veterinary Sciences : open access journal</i> Bd. 9. Basel, MDPI (2022),
    Nr. 11'
  havard: 'S. Doff, J. Wenderlein, A. Wiesinger, S. Hiereth, S. Ulrich, R. Straubinger,
    Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of Canine
    and Equine Origin—A Comparative Study with Two Line Immunoassays, Veterinary Sciences :
    Open Access Journal. 9 (2022).'
  ieee: 'S. Doff, J. Wenderlein, A. Wiesinger, S. Hiereth, S. Ulrich, and R. Straubinger,
    “Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of Canine
    and Equine Origin—A Comparative Study with Two Line Immunoassays,” <i>Veterinary
    Sciences : open access journal</i>, vol. 9, no. 11, Art. no. 633, 2022, doi: <a
    href="https://doi.org/10.3390/vetsci9110633">10.3390/vetsci9110633</a>.'
  mla: 'Doff, Sophie, et al. “Detection of Borrelia Burgdorferi Sensu-Lato-Specific
    Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line
    Immunoassays.” <i>Veterinary Sciences : Open Access Journal</i>, vol. 9, no. 11,
    633, 2022, <a href="https://doi.org/10.3390/vetsci9110633">https://doi.org/10.3390/vetsci9110633</a>.'
  short: 'S. Doff, J. Wenderlein, A. Wiesinger, S. Hiereth, S. Ulrich, R. Straubinger,
    Veterinary Sciences : Open Access Journal 9 (2022).'
  ufg: '<b>Doff, Sophie u. a.</b>: Detection of Borrelia burgdorferi sensu-lato-Specific
    Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line
    Immunoassays, in: <i>Veterinary Sciences : open access journal</i> 9 (2022), H.
    11.'
  van: 'Doff S, Wenderlein J, Wiesinger A, Hiereth S, Ulrich S, Straubinger R. Detection
    of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of Canine and Equine
    Origin—A Comparative Study with Two Line Immunoassays. Veterinary Sciences : open
    access journal. 2022;9(11).'
date_created: 2025-06-15T09:54:05Z
date_updated: 2025-06-16T11:49:06Z
department:
- _id: DEP4010
doi: 10.3390/vetsci9110633
extern: '1'
intvolume: '         9'
issue: '11'
keyword:
- antibody
- Borrelia burgdorferi sensu lato
- canine
- equine
- serum diagnosis
- line immunoassay
language:
- iso: eng
place: Basel
publication: 'Veterinary Sciences : open access journal'
publication_identifier:
  eissn:
  - 2306-7381
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Detection of Borrelia burgdorferi sensu-lato-Specific Antibodies in Sera of
  Canine and Equine Origin—A Comparative Study with Two Line Immunoassays
type: scientific_journal_article
user_id: '83781'
volume: 9
year: '2022'
...
---
_id: '12945'
abstract:
- lang: eng
  text: Stachybotrys chartarum is a toxigenic fungus that is frequently isolated from
    damp building materials or improperly stored forage. Macrocyclic trichothecenes
    and in particular satratoxins are the most potent mycotoxins known to be produced
    by this fungus. Exposure of humans or animals to these secondary metabolites can
    be associated with severe health problems. To assess the pathogenic potential
    of S. chartarum isolates, it is essential to cultivate them under conditions that
    reliably promote toxin production. Potato dextrose agar (PDA) was reported to
    be the optimal nutrition medium for satratoxin production. In this study, the
    growth of S. chartarum genotype S strains on PDA from two manufacturers led to
    divergent results, namely, well-grown and sporulating cultures with high satratoxin
    concentrations (20.8 ± 0.4 µg/cm2) versus cultures with sparse sporulation and
    low satratoxin production (0.3 ± 0.1 µg/cm2). This finding is important for any
    attempt to identify toxigenic S. chartarum isolates. Further experiments performed
    with the two media provided strong evidence for a link between satratoxin production
    and sporulation. A comparison of three-point and one-point cultures grown on the
    two types of PDA, furthermore, demonstrated an inter-colony communication that
    influences both sporulation and mycotoxin production of S. chartarum genotype
    S strains.
article_number: '515'
author:
- first_name: Katharina
  full_name: Tribelhorn, Katharina
  last_name: Tribelhorn
- first_name: Magdalena
  full_name: Twarużek, Magdalena
  last_name: Twarużek
- first_name: Ewelina
  full_name: Soszczyńska, Ewelina
  last_name: Soszczyńska
- first_name: Jörg
  full_name: Rau, Jörg
  last_name: Rau
- first_name: Christiane
  full_name: Baschien, Christiane
  last_name: Baschien
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Frank
  full_name: Ebel, Frank
  last_name: Ebel
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
citation:
  ama: Tribelhorn K, Twarużek M, Soszczyńska E, et al. Production of Satratoxin G
    and H Is Tightly Linked to Sporulation in Stachybotrys chartarum. <i>Toxins</i>.
    2022;14(8). doi:<a href="https://doi.org/10.3390/toxins14080515">10.3390/toxins14080515</a>
  apa: Tribelhorn, K., Twarużek, M., Soszczyńska, E., Rau, J., Baschien, C., Straubinger,
    R. K., Ebel, F., &#38; Ulrich, S. (2022). Production of Satratoxin G and H Is
    Tightly Linked to Sporulation in Stachybotrys chartarum. <i>Toxins</i>, <i>14</i>(8),
    Article 515. <a href="https://doi.org/10.3390/toxins14080515">https://doi.org/10.3390/toxins14080515</a>
  bjps: <b>Tribelhorn K <i>et al.</i></b> (2022) Production of Satratoxin G and H
    Is Tightly Linked to Sporulation in Stachybotrys Chartarum. <i>Toxins</i> <b>14</b>.
  chicago: Tribelhorn, Katharina, Magdalena Twarużek, Ewelina Soszczyńska, Jörg Rau,
    Christiane Baschien, Reinhard K. Straubinger, Frank Ebel, and Sebastian Ulrich.
    “Production of Satratoxin G and H Is Tightly Linked to Sporulation in Stachybotrys
    Chartarum.” <i>Toxins</i> 14, no. 8 (2022). <a href="https://doi.org/10.3390/toxins14080515">https://doi.org/10.3390/toxins14080515</a>.
  chicago-de: Tribelhorn, Katharina, Magdalena Twarużek, Ewelina Soszczyńska, Jörg
    Rau, Christiane Baschien, Reinhard K. Straubinger, Frank Ebel und Sebastian Ulrich.
    2022. Production of Satratoxin G and H Is Tightly Linked to Sporulation in Stachybotrys
    chartarum. <i>Toxins</i> 14, Nr. 8. doi:<a href="https://doi.org/10.3390/toxins14080515">10.3390/toxins14080515</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Tribelhorn, Katharina</span>
    ; <span style="font-variant:small-caps;">Twarużek, Magdalena</span> ; <span style="font-variant:small-caps;">Soszczyńska,
    Ewelina</span> ; <span style="font-variant:small-caps;">Rau, Jörg</span> ; <span
    style="font-variant:small-caps;">Baschien, Christiane</span> ; <span style="font-variant:small-caps;">Straubinger,
    Reinhard K.</span> ; <span style="font-variant:small-caps;">Ebel, Frank</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>: Production
    of Satratoxin G and H Is Tightly Linked to Sporulation in Stachybotrys chartarum.
    In: <i>Toxins</i> Bd. 14, MDPI (2022), Nr. 8'
  havard: K. Tribelhorn, M. Twarużek, E. Soszczyńska, J. Rau, C. Baschien, R.K. Straubinger,
    F. Ebel, S. Ulrich, Production of Satratoxin G and H Is Tightly Linked to Sporulation
    in Stachybotrys chartarum, Toxins. 14 (2022).
  ieee: 'K. Tribelhorn <i>et al.</i>, “Production of Satratoxin G and H Is Tightly
    Linked to Sporulation in Stachybotrys chartarum,” <i>Toxins</i>, vol. 14, no.
    8, Art. no. 515, 2022, doi: <a href="https://doi.org/10.3390/toxins14080515">10.3390/toxins14080515</a>.'
  mla: Tribelhorn, Katharina, et al. “Production of Satratoxin G and H Is Tightly
    Linked to Sporulation in Stachybotrys Chartarum.” <i>Toxins</i>, vol. 14, no.
    8, 515, 2022, <a href="https://doi.org/10.3390/toxins14080515">https://doi.org/10.3390/toxins14080515</a>.
  short: K. Tribelhorn, M. Twarużek, E. Soszczyńska, J. Rau, C. Baschien, R.K. Straubinger,
    F. Ebel, S. Ulrich, Toxins 14 (2022).
  ufg: '<b>Tribelhorn, Katharina u. a.</b>: Production of Satratoxin G and H Is Tightly
    Linked to Sporulation in Stachybotrys chartarum, in: <i>Toxins</i> 14 (2022),
    H. 8.'
  van: Tribelhorn K, Twarużek M, Soszczyńska E, Rau J, Baschien C, Straubinger RK,
    et al. Production of Satratoxin G and H Is Tightly Linked to Sporulation in Stachybotrys
    chartarum. Toxins. 2022;14(8).
date_created: 2025-06-15T09:56:06Z
date_updated: 2025-06-16T11:52:01Z
department:
- _id: DEP4010
doi: 10.3390/toxins14080515
extern: '1'
intvolume: '        14'
issue: '8'
keyword:
- Stachybotrys chartarum genotype S
- sporulation
- satratoxins
- macrocyclic trichothecenes
- inter-colony communication
language:
- iso: eng
publication: Toxins
publication_identifier:
  eissn:
  - 2072-6651
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Production of Satratoxin G and H Is Tightly Linked to Sporulation in Stachybotrys
  chartarum
type: scientific_journal_article
user_id: '83781'
volume: 14
year: '2022'
...
---
_id: '12946'
abstract:
- lang: eng
  text: Ostrich meat is characterized by high nutritional value; however, it remains
    an exotic product in most countries worldwide. In Europe, only few data are available
    regarding its microbial contamination, prevalence of antimicrobial-resistant bacteria,
    and safety. Therefore, this study aimed to investigate the microbiological quality
    and safety of ostrich meat samples (n = 55), each from one animal, produced in
    Bavaria, Germany. The provided microbiological status of ostrich meat included
    mesophilic aerobic bacteria, Enterobacteria, and mesophilic yeast and molds. In
    terms of food safety, all meat samples were negative for Salmonella spp. and Trichinella
    spp. Additionally, meat samples and a further 30 stool samples from 30 individuals
    were investigated for Shiga toxin-producing Escherichia coli genes, with two meat
    samples that were qPCR-positive. Antimicrobial-resistant Enterobacteriaceae, Enterococcus
    faecalis, and Enterococcus faecium strains were from meat and stool samples also
    analyzed; 13 potentially resistant Enterobacteriaceae (meat samples) and 4 Enterococcus
    faecium (stool samples) were isolated, and their susceptibility against 29 and
    14 antimicrobials, respectively, was characterized. The results of this study
    provide an overview of microbial loads and food safety aspects that may be used
    as baseline data for the ostrich meat industry to improve their hygienic quality.
    However, the implementation of monitoring programs is recommended, and microbiological
    standards for ostrich meat production should be established.
article_number: '985'
author:
- first_name: Philipp-Michael
  full_name: Beindorf, Philipp-Michael
  last_name: Beindorf
- first_name: Oksana
  full_name: Kovalenko, Oksana
  last_name: Kovalenko
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Hanna
  full_name: Geißler, Hanna
  last_name: Geißler
- first_name: Rüdiger
  full_name: Korbel, Rüdiger
  last_name: Korbel
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Samart
  full_name: Dorn-In, Samart
  last_name: Dorn-In
- first_name: Irene
  full_name: Esteban-Cuesta, Irene
  last_name: Esteban-Cuesta
citation:
  ama: 'Beindorf PM, Kovalenko O, Ulrich S, et al. Investigation of Meat from Ostriches
    Raised and Slaughtered in Bavaria, Germany: Microbiological Quality and Antimicrobial
    Resistance. <i>Biology : open access journal</i>. 2022;11(7). doi:<a href="https://doi.org/10.3390/biology11070985">10.3390/biology11070985</a>'
  apa: 'Beindorf, P.-M., Kovalenko, O., Ulrich, S., Geißler, H., Korbel, R., Schwaiger,
    K., Dorn-In, S., &#38; Esteban-Cuesta, I. (2022). Investigation of Meat from Ostriches
    Raised and Slaughtered in Bavaria, Germany: Microbiological Quality and Antimicrobial
    Resistance. <i>Biology : Open Access Journal</i>, <i>11</i>(7), Article 985. <a
    href="https://doi.org/10.3390/biology11070985">https://doi.org/10.3390/biology11070985</a>'
  bjps: '<b>Beindorf P-M <i>et al.</i></b> (2022) Investigation of Meat from Ostriches
    Raised and Slaughtered in Bavaria, Germany: Microbiological Quality and Antimicrobial
    Resistance. <i>Biology : open access journal</i> <b>11</b>.'
  chicago: 'Beindorf, Philipp-Michael, Oksana Kovalenko, Sebastian Ulrich, Hanna Geißler,
    Rüdiger Korbel, Karin Schwaiger, Samart Dorn-In, and Irene Esteban-Cuesta. “Investigation
    of Meat from Ostriches Raised and Slaughtered in Bavaria, Germany: Microbiological
    Quality and Antimicrobial Resistance.” <i>Biology : Open Access Journal</i> 11,
    no. 7 (2022). <a href="https://doi.org/10.3390/biology11070985">https://doi.org/10.3390/biology11070985</a>.'
  chicago-de: 'Beindorf, Philipp-Michael, Oksana Kovalenko, Sebastian Ulrich, Hanna
    Geißler, Rüdiger Korbel, Karin Schwaiger, Samart Dorn-In und Irene Esteban-Cuesta.
    2022. Investigation of Meat from Ostriches Raised and Slaughtered in Bavaria,
    Germany: Microbiological Quality and Antimicrobial Resistance. <i>Biology : open
    access journal</i> 11, Nr. 7. doi:<a href="https://doi.org/10.3390/biology11070985">10.3390/biology11070985</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Beindorf, Philipp-Michael</span>
    ; <span style="font-variant:small-caps;">Kovalenko, Oksana</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Geißler, Hanna</span>
    ; <span style="font-variant:small-caps;">Korbel, Rüdiger</span> ; <span style="font-variant:small-caps;">Schwaiger,
    Karin</span> ; <span style="font-variant:small-caps;">Dorn-In, Samart</span> ;
    <span style="font-variant:small-caps;">Esteban-Cuesta, Irene</span>: Investigation
    of Meat from Ostriches Raised and Slaughtered in Bavaria, Germany: Microbiological
    Quality and Antimicrobial Resistance. In: <i>Biology : open access journal</i>
    Bd. 11. Basel, MDPI (2022), Nr. 7'
  havard: 'P.-M. Beindorf, O. Kovalenko, S. Ulrich, H. Geißler, R. Korbel, K. Schwaiger,
    S. Dorn-In, I. Esteban-Cuesta, Investigation of Meat from Ostriches Raised and
    Slaughtered in Bavaria, Germany: Microbiological Quality and Antimicrobial Resistance,
    Biology : Open Access Journal. 11 (2022).'
  ieee: 'P.-M. Beindorf <i>et al.</i>, “Investigation of Meat from Ostriches Raised
    and Slaughtered in Bavaria, Germany: Microbiological Quality and Antimicrobial
    Resistance,” <i>Biology : open access journal</i>, vol. 11, no. 7, Art. no. 985,
    2022, doi: <a href="https://doi.org/10.3390/biology11070985">10.3390/biology11070985</a>.'
  mla: 'Beindorf, Philipp-Michael, et al. “Investigation of Meat from Ostriches Raised
    and Slaughtered in Bavaria, Germany: Microbiological Quality and Antimicrobial
    Resistance.” <i>Biology : Open Access Journal</i>, vol. 11, no. 7, 985, 2022,
    <a href="https://doi.org/10.3390/biology11070985">https://doi.org/10.3390/biology11070985</a>.'
  short: 'P.-M. Beindorf, O. Kovalenko, S. Ulrich, H. Geißler, R. Korbel, K. Schwaiger,
    S. Dorn-In, I. Esteban-Cuesta, Biology : Open Access Journal 11 (2022).'
  ufg: '<b>Beindorf, Philipp-Michael u. a.</b>: Investigation of Meat from Ostriches
    Raised and Slaughtered in Bavaria, Germany: Microbiological Quality and Antimicrobial
    Resistance, in: <i>Biology : open access journal</i> 11 (2022), H. 7.'
  van: 'Beindorf PM, Kovalenko O, Ulrich S, Geißler H, Korbel R, Schwaiger K, et al.
    Investigation of Meat from Ostriches Raised and Slaughtered in Bavaria, Germany:
    Microbiological Quality and Antimicrobial Resistance. Biology : open access journal.
    2022;11(7).'
date_created: 2025-06-15T09:56:52Z
date_updated: 2025-06-16T11:55:55Z
department:
- _id: DEP4010
doi: 10.3390/biology11070985
extern: '1'
intvolume: '        11'
issue: '7'
keyword:
- antimicrobial resistance
- meat microbiology
- Salmonella
- STEC
- Trichinella
language:
- iso: eng
place: Basel
publication: 'Biology : open access journal'
publication_identifier:
  eissn:
  - 2079-7737
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: 'Investigation of Meat from Ostriches Raised and Slaughtered in Bavaria, Germany:
  Microbiological Quality and Antimicrobial Resistance'
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2022'
...
---
_id: '12947'
abstract:
- lang: eng
  text: 'Stachybotrys chartarum is frequently isolated from damp building materials
    or improperly stored animal forage. Human and animal exposure to the secondary
    metabolites of this mold is linked to severe health effects. The mutually exclusive
    production of either satratoxins or atranones defines the chemotypes A and S.
    Based upon the genes (satratoxin cluster, SC1-3, sat or atranone cluster, AC1,
    atr) that are supposed to be essential for satratoxin and atranone production,
    S. chartarum can furthermore be divided into three genotypes: the S-type possessing
    all sat- but no atr-genes, the A-type lacking the sat- but harboring all atr-genes,
    and the H-type having only certain sat- and all atr-genes. We analyzed the above-mentioned
    gene clusters and their flanking regions to shed light on the evolutionary relationship.
    Furthermore, we performed a deep re-sequencing and LC-MS/MS (Liquid chromatography–mass
    spectrometry) analysis. We propose a first model for the evolution of the S. chartarum
    genotypes. We assume that genotype H represents the most ancient form. A loss
    of the AC1 and the concomitant acquisition of the SC2 led to the emergence of
    the genotype S. According to our model, the genotype H also developed towards
    genotype A, a process that was accompanied by a loss of SC1 and SC3.'
article_number: '340'
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Katharina
  full_name: Lang, Katharina
  last_name: Lang
- first_name: Ludwig
  full_name: Niessen, Ludwig
  last_name: Niessen
- first_name: Christiane
  full_name: Baschien, Christiane
  last_name: Baschien
- first_name: Robert
  full_name: Kosicki, Robert
  last_name: Kosicki
- first_name: Magdalena
  full_name: Twarużek, Magdalena
  last_name: Twarużek
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Frank
  full_name: Ebel, Frank
  last_name: Ebel
citation:
  ama: Ulrich S, Lang K, Niessen L, et al. The Evolution of the Satratoxin and Atranone
    Gene Clusters of Stachybotrys chartarum. <i>Journal of Fungi</i>. 2022;8(4). doi:<a
    href="https://doi.org/10.3390/jof8040340">10.3390/jof8040340</a>
  apa: Ulrich, S., Lang, K., Niessen, L., Baschien, C., Kosicki, R., Twarużek, M.,
    Straubinger, R. K., &#38; Ebel, F. (2022). The Evolution of the Satratoxin and
    Atranone Gene Clusters of Stachybotrys chartarum. <i>Journal of Fungi</i>, <i>8</i>(4),
    Article 340. <a href="https://doi.org/10.3390/jof8040340">https://doi.org/10.3390/jof8040340</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2022) The Evolution of the Satratoxin and Atranone
    Gene Clusters of Stachybotrys Chartarum. <i>Journal of Fungi</i> <b>8</b>.
  chicago: Ulrich, Sebastian, Katharina Lang, Ludwig Niessen, Christiane Baschien,
    Robert Kosicki, Magdalena Twarużek, Reinhard K. Straubinger, and Frank Ebel. “The
    Evolution of the Satratoxin and Atranone Gene Clusters of Stachybotrys Chartarum.”
    <i>Journal of Fungi</i> 8, no. 4 (2022). <a href="https://doi.org/10.3390/jof8040340">https://doi.org/10.3390/jof8040340</a>.
  chicago-de: Ulrich, Sebastian, Katharina Lang, Ludwig Niessen, Christiane Baschien,
    Robert Kosicki, Magdalena Twarużek, Reinhard K. Straubinger und Frank Ebel. 2022.
    The Evolution of the Satratoxin and Atranone Gene Clusters of Stachybotrys chartarum.
    <i>Journal of Fungi</i> 8, Nr. 4. doi:<a href="https://doi.org/10.3390/jof8040340">10.3390/jof8040340</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Lang, Katharina</span> ; <span style="font-variant:small-caps;">Niessen,
    Ludwig</span> ; <span style="font-variant:small-caps;">Baschien, Christiane</span>
    ; <span style="font-variant:small-caps;">Kosicki, Robert</span> ; <span style="font-variant:small-caps;">Twarużek,
    Magdalena</span> ; <span style="font-variant:small-caps;">Straubinger, Reinhard
    K.</span> ; <span style="font-variant:small-caps;">Ebel, Frank</span>: The Evolution
    of the Satratoxin and Atranone Gene Clusters of Stachybotrys chartarum. In: <i>Journal
    of Fungi</i> Bd. 8, MDPI AG (2022), Nr. 4'
  havard: S. Ulrich, K. Lang, L. Niessen, C. Baschien, R. Kosicki, M. Twarużek, R.K.
    Straubinger, F. Ebel, The Evolution of the Satratoxin and Atranone Gene Clusters
    of Stachybotrys chartarum, Journal of Fungi. 8 (2022).
  ieee: 'S. Ulrich <i>et al.</i>, “The Evolution of the Satratoxin and Atranone Gene
    Clusters of Stachybotrys chartarum,” <i>Journal of Fungi</i>, vol. 8, no. 4, Art.
    no. 340, 2022, doi: <a href="https://doi.org/10.3390/jof8040340">10.3390/jof8040340</a>.'
  mla: Ulrich, Sebastian, et al. “The Evolution of the Satratoxin and Atranone Gene
    Clusters of Stachybotrys Chartarum.” <i>Journal of Fungi</i>, vol. 8, no. 4, 340,
    2022, <a href="https://doi.org/10.3390/jof8040340">https://doi.org/10.3390/jof8040340</a>.
  short: S. Ulrich, K. Lang, L. Niessen, C. Baschien, R. Kosicki, M. Twarużek, R.K.
    Straubinger, F. Ebel, Journal of Fungi 8 (2022).
  ufg: '<b>Ulrich, Sebastian u. a.</b>: The Evolution of the Satratoxin and Atranone
    Gene Clusters of Stachybotrys chartarum, in: <i>Journal of Fungi</i> 8 (2022),
    H. 4.'
  van: Ulrich S, Lang K, Niessen L, Baschien C, Kosicki R, Twarużek M, et al. The
    Evolution of the Satratoxin and Atranone Gene Clusters of Stachybotrys chartarum.
    Journal of Fungi. 2022;8(4).
date_created: 2025-06-15T09:57:20Z
date_updated: 2025-06-16T11:59:52Z
department:
- _id: DEP4010
doi: 10.3390/jof8040340
extern: '1'
intvolume: '         8'
issue: '4'
keyword:
- Stachybotrys
- genome
- macrocyclic trichothecene
- atranone
language:
- iso: eng
publication: Journal of Fungi
publication_identifier:
  eissn:
  - 2309-608X
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: The Evolution of the Satratoxin and Atranone Gene Clusters of Stachybotrys
  chartarum
type: scientific_journal_article
user_id: '83781'
volume: 8
year: '2022'
...
---
_id: '12948'
abstract:
- lang: eng
  text: Diet processing impacts on starch properties, such as the degree of starch
    gelatinization. This affects digestibility, as shown in laboratory mice fed either
    a pelleted or an extruded diet. In the present study, the morphology of starch
    particles throughout the digestive tract of mice was visualized. Thirty-two female
    C57BL/6J mice were used for a feeding trial. They were fed a commercial maintenance
    diet for laboratory mice, which was available in pelleted and extruded form, for
    seven weeks. The mice were sacrificed after the feeding period, and chyme samples
    were collected from five sites (stomach, anterior and posterior small intestine,
    caecum, colon). Samples of diets, chyme and faeces were analyzed via stereomicroscopy
    (stained with Lugol’s iodine) and scanning electron microscopy (SEM). The starch
    granules appeared more compact in the pelleted diet, showing first signs of degradation
    only in the small intestine. The caecum content of both diets group was intensively
    stained, particles as well as fluid phase, indicating that it contained mainly
    starch. The SEM pictures of caecum content showed abundant bacteria near starch
    particles. This suggests selective retention of prae-caecally undigested starch
    in the murine caecum, likely the site of microbial fermentation.
article_number: '952'
author:
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Ellen
  full_name: Kienzle, Ellen
  last_name: Kienzle
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Heidrun
  full_name: Schöl, Heidrun
  last_name: Schöl
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Linda Franziska
  full_name: Böswald, Linda Franziska
  last_name: Böswald
citation:
  ama: Wenderlein J, Kienzle E, Straubinger RK, Schöl H, Ulrich S, Böswald LF. Morphology
    of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory
    Mice Fed Extruded and Pelleted Diets. <i>Animals</i>. 2022;12(8). doi:<a href="https://doi.org/10.3390/ani12080952">10.3390/ani12080952</a>
  apa: Wenderlein, J., Kienzle, E., Straubinger, R. K., Schöl, H., Ulrich, S., &#38;
    Böswald, L. F. (2022). Morphology of Starch Particles along the Passage through
    the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets.
    <i>Animals</i>, <i>12</i>(8), Article 952. <a href="https://doi.org/10.3390/ani12080952">https://doi.org/10.3390/ani12080952</a>
  bjps: <b>Wenderlein J <i>et al.</i></b> (2022) Morphology of Starch Particles along
    the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded
    and Pelleted Diets. <i>Animals</i> <b>12</b>.
  chicago: Wenderlein, Jasmin, Ellen Kienzle, Reinhard K. Straubinger, Heidrun Schöl,
    Sebastian Ulrich, and Linda Franziska Böswald. “Morphology of Starch Particles
    along the Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded
    and Pelleted Diets.” <i>Animals</i> 12, no. 8 (2022). <a href="https://doi.org/10.3390/ani12080952">https://doi.org/10.3390/ani12080952</a>.
  chicago-de: Wenderlein, Jasmin, Ellen Kienzle, Reinhard K. Straubinger, Heidrun
    Schöl, Sebastian Ulrich und Linda Franziska Böswald. 2022. Morphology of Starch
    Particles along the Passage through the Gastrointestinal Tract in Laboratory Mice
    Fed Extruded and Pelleted Diets. <i>Animals</i> 12, Nr. 8. doi:<a href="https://doi.org/10.3390/ani12080952">10.3390/ani12080952</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Wenderlein, Jasmin</span> ;
    <span style="font-variant:small-caps;">Kienzle, Ellen</span> ; <span style="font-variant:small-caps;">Straubinger,
    Reinhard K.</span> ; <span style="font-variant:small-caps;">Schöl, Heidrun</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Böswald,
    Linda Franziska</span>: Morphology of Starch Particles along the Passage through
    the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted Diets.
    In: <i>Animals</i> Bd. 12. Basel, MDPI AG (2022), Nr. 8'
  havard: J. Wenderlein, E. Kienzle, R.K. Straubinger, H. Schöl, S. Ulrich, L.F. Böswald,
    Morphology of Starch Particles along the Passage through the Gastrointestinal
    Tract in Laboratory Mice Fed Extruded and Pelleted Diets, Animals. 12 (2022).
  ieee: 'J. Wenderlein, E. Kienzle, R. K. Straubinger, H. Schöl, S. Ulrich, and L.
    F. Böswald, “Morphology of Starch Particles along the Passage through the Gastrointestinal
    Tract in Laboratory Mice Fed Extruded and Pelleted Diets,” <i>Animals</i>, vol.
    12, no. 8, Art. no. 952, 2022, doi: <a href="https://doi.org/10.3390/ani12080952">10.3390/ani12080952</a>.'
  mla: Wenderlein, Jasmin, et al. “Morphology of Starch Particles along the Passage
    through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and Pelleted
    Diets.” <i>Animals</i>, vol. 12, no. 8, 952, 2022, <a href="https://doi.org/10.3390/ani12080952">https://doi.org/10.3390/ani12080952</a>.
  short: J. Wenderlein, E. Kienzle, R.K. Straubinger, H. Schöl, S. Ulrich, L.F. Böswald,
    Animals 12 (2022).
  ufg: '<b>Wenderlein, Jasmin u. a.</b>: Morphology of Starch Particles along the
    Passage through the Gastrointestinal Tract in Laboratory Mice Fed Extruded and
    Pelleted Diets, in: <i>Animals</i> 12 (2022), H. 8.'
  van: Wenderlein J, Kienzle E, Straubinger RK, Schöl H, Ulrich S, Böswald LF. Morphology
    of Starch Particles along the Passage through the Gastrointestinal Tract in Laboratory
    Mice Fed Extruded and Pelleted Diets. Animals. 2022;12(8).
date_created: 2025-06-15T09:57:56Z
date_updated: 2025-06-16T12:01:46Z
department:
- _id: DEP4010
doi: 10.3390/ani12080952
extern: '1'
intvolume: '        12'
issue: '8'
keyword:
- amylase
- carbohydrate metabolism
- processing
- laboratory animal diets
- caecum fermentation
language:
- iso: eng
place: Basel
publication: Animals
publication_identifier:
  eissn:
  - 2076-2615
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: Morphology of Starch Particles along the Passage through the Gastrointestinal
  Tract in Laboratory Mice Fed Extruded and Pelleted Diets
type: scientific_journal_article
user_id: '83781'
volume: 12
year: '2022'
...
---
_id: '12957'
abstract:
- lang: eng
  text: "Mit dieser Arbeit sollte die klinische Relevanz von Infektionen mit Borrelia
    burgdorferi sensu lato (Bbsl) sowie Anaplas-\r\nma phagocytophilum (Ap) bei Pferden
    in Deutschland untersucht und mögliche Assoziationen zwischen typischen klinischen
    Veränderungen und spezifischen erhöhten Serumantikörperspiegeln gefunden werden.
    Hierfür wurden Pferde mit dem Verdacht auf eine klinisch manifeste Lyme-Borreliose
    (LB) deutschlandweit untersucht. Die Tierärzte wurden zudem gebeten, einen Befund-
    bzw. Fragebogen auszufüllen. Neben einer Blutprobe von dem LB-erkrankten bzw.
    -verdächtigen Pferd wurde immer auch eine Blutprobe eines gesunden Kontrollpferdes
    aus dem gleichen Bestand entnommen. Die Blutproben wurden mittels ELISA (enzyme-linked
    immunosorbent assay) und Line-Immunoassay auf spezifische Antikörper (Ak) gegen
    Bbsl und zusätzlich mittels eines validierten SNAP-Tests (SNAP® 4Dx Plus® ELISA)
    auf spezifische Ak für Bbsl und Anaplasma phagocytophilum (Ap) untersucht. Zudem
    wurde ein manueller Ausstrich auf Einschlusskörperchen in den Granulozyten, die
    auf eine Ap-Infektion hindeuten, untersucht. Insgesamt wurden 123 LB-Verdachts-
    und 113 Kontrollpferde an der Studie aufgenommen. 114 Tierarztfragebögen und Blutproben
    lagen vollständig vor und gingen in die statistische Auswertung ein. Die häufigsten
    Vorstellungsgründe der LB-Verdachtstiere waren Lahmheit (n = 36; nges = 79; 45,6
    %), Leistungsschwäche (n = 19; 24,1 %) und Apathie (n = 12; 15,2 %). Bei fast
    der Hälfte der Patienten wurden die klinischen Veränderungen bereits seit über
    sechs Monaten beobachtet (n = 48; nges = 112; 42,9 %). Zahlreiche Tiere zeigten
    mehrere, oftmals unspezifische klinische Veränderungen (n = 104; nges = 114; 92,2
    %) und/oder litten zusätzlich unter einer chronischen Erkrankung (n = 48; nges
    = 114; 42,5 %). Obwohl in vielen Fällen schon eine weiterführende Infektionsdiagnostik
    (n = 64 von nges = 114; 56,1 %) durchgeführt worden war, wurden einige Pferde
    bislang noch nicht weitergehend labordiagnostisch untersucht (n = 14; nges = 114;
    12,3 %). Bei 15 % der Probanden (n = 29; nges = 112) war zudem bislang noch keine
    eingehende Untersuchung einzelner Organsysteme erfolgt. Auf Basis der eigenen
    serologischen Befunde wurden 51 % (n = 63) der LB Verdachtspferde negativ (49
    % der Verdachtspferde; n = 55), 28 % (n = 34) wurden grenzwertig (35 % der Kontrollpferde;
    n = 40) und 21 % (n = 26) positiv (16 % der Kontrollpferde; n = 18) auf spezifische
    Ak gegen Bbsl getestet. Ein positiver Ap-spezifischer Ak-Nachweis lag bei 19,5
    % der Verdachtspferde (16,8 % der Kontrollpferde) vor. Ein Hinweis auf eine Coinfektion
    mit Bbsl und Ap konnte bei sieben Verdachtstieren (5,7 %; 2 Kontrollpferde, 1.8
    %) gefunden werden. Die Blutausstriche waren bei allen Verdachts- und Kontrolltieren,
    bei denen sie auswertbar waren (n = 98), ohne besonderen Befund. Die hohe Zahl
    klinisch inapparenter Verläufe von Infektionen mit Bbsl konnte durch die hohe
    Zahl seropositiver Probanden (n = 18; nges = 112; 16 %)\r\nin der gesunden Kontrollgruppe
    (KG) bestätigt werden. Insgesamt ergab sich ein kaum differierender Serostatus
    von Verdachts- und Kontrollpferden (p = 0,887). Es konnten mit dem gewonnenen
    Datenmaterial keine pathognomonisch definierten, klinischen Veränderungen für
    die LB bei Pferden herausgearbeitet werden. Weder das gehäufte Auftreten unspezifischer
    Störungen des Allgemeinbefindens (p = 0,043), noch Lahmheiten (p = 0,782) oder
    Gelenkschwellungen (p = 0,013) konnten statistisch signifikant im Zusammenhang
    mit positivem Bbsl-Ak-Nachweis beobachtet werden. Die Chance für einen positiven
    Ap-Ak-Nachweis war bei Fieber (OR = 3,54 (1,28–9,73)) und Inappetenz (OR = 4,54
    (1,44–14,29)) erhöht. Bei Coinfektionen (Bbsl + Ap) wurden zudem auffallend häufig
    neurologische Veränderungen, wie Kopfnervenausfälle (p = 0,030) und Hinweise auf
    eine Meningoenzephalitis (p = 0,003) diagnostiziert, wobei letztere Korrelation
    aufgrund der geringen Anzahl\r\nan betroffenen Patienten mit einer Unsicherheit
    hinsichtlich der Praxisrelevanz behaftet ist."
author:
- first_name: Heidrun
  full_name: Gehlen, Heidrun
  last_name: Gehlen
- first_name: Katharina S
  full_name: Inerle, Katharina S
  last_name: Inerle
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Beatrice
  full_name: Briese, Beatrice
  last_name: Briese
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: Gehlen H, Inerle KS, Ulrich S, Briese B, Straubinger RK. Lyme-Borreliose und
    Granulozytäre Anaplasmose bei Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung).
    <i>Pferdeheilkunde </i>. 2022;38(6):544-553. doi:<a href="https://doi.org/10.21836/PEM20220606">10.21836/PEM20220606</a>
  apa: Gehlen, H., Inerle, K. S., Ulrich, S., Briese, B., &#38; Straubinger, R. K.
    (2022). Lyme-Borreliose und Granulozytäre Anaplasmose bei Pferden Teil 2 – Klinische
    Relevanz (Tierarztbefragung). <i>Pferdeheilkunde </i>, <i>38</i>(6), 544–553.
    <a href="https://doi.org/10.21836/PEM20220606">https://doi.org/10.21836/PEM20220606</a>
  bjps: <b>Gehlen H <i>et al.</i></b> (2022) Lyme-Borreliose und Granulozytäre Anaplasmose
    bei Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung). <i>Pferdeheilkunde
    </i> <b>38</b>, 544–553.
  chicago: 'Gehlen, Heidrun, Katharina S Inerle, Sebastian Ulrich, Beatrice Briese,
    and Reinhard K. Straubinger. “Lyme-Borreliose und Granulozytäre Anaplasmose bei
    Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung).” <i>Pferdeheilkunde </i>
    38, no. 6 (2022): 544–53. <a href="https://doi.org/10.21836/PEM20220606">https://doi.org/10.21836/PEM20220606</a>.'
  chicago-de: 'Gehlen, Heidrun, Katharina S Inerle, Sebastian Ulrich, Beatrice Briese
    und Reinhard K. Straubinger. 2022. Lyme-Borreliose und Granulozytäre Anaplasmose
    bei Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung). <i>Pferdeheilkunde
    </i> 38, Nr. 6: 544–553. doi:<a href="https://doi.org/10.21836/PEM20220606">10.21836/PEM20220606</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Gehlen, Heidrun</span> ; <span
    style="font-variant:small-caps;">Inerle, Katharina S</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Briese, Beatrice</span>
    ; <span style="font-variant:small-caps;">Straubinger, Reinhard K.</span>: Lyme-Borreliose
    und Granulozytäre Anaplasmose bei Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung).
    In: <i>Pferdeheilkunde </i> Bd. 38. Stuttgart, Hippiatrika-Verl. GmbH  (2022),
    Nr. 6, S. 544–553'
  havard: H. Gehlen, K.S. Inerle, S. Ulrich, B. Briese, R.K. Straubinger, Lyme-Borreliose
    und Granulozytäre Anaplasmose bei Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung),
    Pferdeheilkunde . 38 (2022) 544–553.
  ieee: 'H. Gehlen, K. S. Inerle, S. Ulrich, B. Briese, and R. K. Straubinger, “Lyme-Borreliose
    und Granulozytäre Anaplasmose bei Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung),”
    <i>Pferdeheilkunde </i>, vol. 38, no. 6, pp. 544–553, 2022, doi: <a href="https://doi.org/10.21836/PEM20220606">10.21836/PEM20220606</a>.'
  mla: Gehlen, Heidrun, et al. “Lyme-Borreliose und Granulozytäre Anaplasmose bei
    Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung).” <i>Pferdeheilkunde </i>,
    vol. 38, no. 6, 2022, pp. 544–53, <a href="https://doi.org/10.21836/PEM20220606">https://doi.org/10.21836/PEM20220606</a>.
  short: H. Gehlen, K.S. Inerle, S. Ulrich, B. Briese, R.K. Straubinger, Pferdeheilkunde  38
    (2022) 544–553.
  ufg: '<b>Gehlen, Heidrun u. a.</b>: Lyme-Borreliose und Granulozytäre Anaplasmose
    bei Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung), in: <i>Pferdeheilkunde
    </i> 38 (2022), H. 6,  S. 544–553.'
  van: Gehlen H, Inerle KS, Ulrich S, Briese B, Straubinger RK. Lyme-Borreliose und
    Granulozytäre Anaplasmose bei Pferden Teil 2 – Klinische Relevanz (Tierarztbefragung).
    Pferdeheilkunde . 2022;38(6):544–53.
date_created: 2025-06-15T10:05:27Z
date_updated: 2025-06-16T14:12:46Z
department:
- _id: DEP4010
doi: 10.21836/PEM20220606
extern: '1'
intvolume: '        38'
issue: '6'
keyword:
- Equine Lyme-Borreliose
- Equine Granulozytäre Anaplasmose
- Seroprävalenz
- Coinfektion
language:
- iso: ger
main_file_link:
- open_access: '1'
  url: https://pferdeheilkunde.de/files/20220606.pdf
oa: '1'
page: 544–553
place: Stuttgart
publication: 'Pferdeheilkunde '
publication_identifier:
  eissn:
  - '2943-1794 '
  issn:
  - 0177-7726
publication_status: published
publisher: 'Hippiatrika-Verl. GmbH '
status: public
title: Lyme-Borreliose und Granulozytäre Anaplasmose bei Pferden Teil 2 – Klinische
  Relevanz (Tierarztbefragung)
type: scientific_journal_article
user_id: '83781'
volume: 38
year: '2022'
...
---
_id: '12950'
abstract:
- lang: eng
  text: The composition of the microbiome is subject to the host’s diet. In commercial
    laboratory mouse diets, different physical forms of the same diets are available,
    containing—according to their labels—identical ingredients and nutrient compositions.
    However, variations in nutrient composition and starch gelatinization due to production
    processes and their impact on digestibility have been described. In this study,
    a total of 48 C57BL/J6 mice were assigned to two equal groups and were fed diets
    (produced with different processes—extruded vs. pelleted) for eight weeks in two
    biological replicates. At the end of the experiment, samples were collected from
    five different gastrointestinal regions, including the stomach, small intestine,
    cecum, large intestine, and an extracorporeal region (feces), and the microbiome
    was analyzed with 16S rRNA gene amplicon sequencing. The replicates in both experiments
    differed significantly in their relative abundances of Muribaculaceae species.
    Furthermore, the gastrointestinal content of pellet-fed mice contained larger
    numbers of Lactobacillus species. These results indicate that starch gelatinization
    and ingredient composition significantly influence microbial makeup. In conclusion,
    different feed processing methods may affect fundamental digestive and metabolic
    processes, impacting animal experiments and biasing microbiome data.
article_number: '862'
author:
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Linda F.
  full_name: Böswald, Linda F.
  last_name: Böswald
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Ellen
  full_name: Kienzle, Ellen
  last_name: Kienzle
- first_name: Klaus
  full_name: Neuhaus, Klaus
  last_name: Neuhaus
- first_name: Ilias
  full_name: Lagkouvardos, Ilias
  last_name: Lagkouvardos
- first_name: Christian
  full_name: Zenner, Christian
  last_name: Zenner
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: Wenderlein J, Böswald LF, Ulrich S, et al. Processing Matters in Nutrient-Matched
    Laboratory Diets for Mice—Microbiome. <i>Animals</i>. 2021;11(3). doi:<a href="https://doi.org/10.3390/ani11030862">10.3390/ani11030862</a>
  apa: Wenderlein, J., Böswald, L. F., Ulrich, S., Kienzle, E., Neuhaus, K., Lagkouvardos,
    I., Zenner, C., &#38; Straubinger, R. K. (2021). Processing Matters in Nutrient-Matched
    Laboratory Diets for Mice—Microbiome. <i>Animals</i>, <i>11</i>(3), Article 862.
    <a href="https://doi.org/10.3390/ani11030862">https://doi.org/10.3390/ani11030862</a>
  bjps: <b>Wenderlein J <i>et al.</i></b> (2021) Processing Matters in Nutrient-Matched
    Laboratory Diets for Mice—Microbiome. <i>Animals</i> <b>11</b>.
  chicago: Wenderlein, Jasmin, Linda F. Böswald, Sebastian Ulrich, Ellen Kienzle,
    Klaus Neuhaus, Ilias Lagkouvardos, Christian Zenner, and Reinhard K. Straubinger.
    “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome.”
    <i>Animals</i> 11, no. 3 (2021). <a href="https://doi.org/10.3390/ani11030862">https://doi.org/10.3390/ani11030862</a>.
  chicago-de: Wenderlein, Jasmin, Linda F. Böswald, Sebastian Ulrich, Ellen Kienzle,
    Klaus Neuhaus, Ilias Lagkouvardos, Christian Zenner und Reinhard K. Straubinger.
    2021. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome.
    <i>Animals</i> 11, Nr. 3. doi:<a href="https://doi.org/10.3390/ani11030862">10.3390/ani11030862</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Wenderlein, Jasmin</span> ;
    <span style="font-variant:small-caps;">Böswald, Linda F.</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Kienzle, Ellen</span>
    ; <span style="font-variant:small-caps;">Neuhaus, Klaus</span> ; <span style="font-variant:small-caps;">Lagkouvardos,
    Ilias</span> ; <span style="font-variant:small-caps;">Zenner, Christian</span>
    ; <span style="font-variant:small-caps;">Straubinger, Reinhard K.</span>: Processing
    Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome. In: <i>Animals</i>
    Bd. 11. Basel, MDPI AG (2021), Nr. 3'
  havard: J. Wenderlein, L.F. Böswald, S. Ulrich, E. Kienzle, K. Neuhaus, I. Lagkouvardos,
    C. Zenner, R.K. Straubinger, Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Microbiome, Animals. 11 (2021).
  ieee: 'J. Wenderlein <i>et al.</i>, “Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Microbiome,” <i>Animals</i>, vol. 11, no. 3, Art. no. 862, 2021,
    doi: <a href="https://doi.org/10.3390/ani11030862">10.3390/ani11030862</a>.'
  mla: Wenderlein, Jasmin, et al. “Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Microbiome.” <i>Animals</i>, vol. 11, no. 3, 862, 2021, <a href="https://doi.org/10.3390/ani11030862">https://doi.org/10.3390/ani11030862</a>.
  short: J. Wenderlein, L.F. Böswald, S. Ulrich, E. Kienzle, K. Neuhaus, I. Lagkouvardos,
    C. Zenner, R.K. Straubinger, Animals 11 (2021).
  ufg: '<b>Wenderlein, Jasmin u. a.</b>: Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Microbiome, in: <i>Animals</i> 11 (2021), H. 3.'
  van: Wenderlein J, Böswald LF, Ulrich S, Kienzle E, Neuhaus K, Lagkouvardos I, et
    al. Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome.
    Animals. 2021;11(3).
date_created: 2025-06-15T09:58:59Z
date_updated: 2025-06-16T12:07:21Z
department:
- _id: DEP4010
doi: 10.3390/ani11030862
extern: '1'
intvolume: '        11'
issue: '3'
keyword:
- feed processing
- starch gelatinization
- laboratory mouse
- diet
- intestinal microbiome
language:
- iso: eng
place: Basel
publication: Animals
publication_identifier:
  issn:
  - 2076-2615
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2021'
...
---
_id: '12951'
abstract:
- lang: eng
  text: Leptospirosis is a neglected worldwide zoonotic bacterial disease with a high
    prevalence in subtropical and tropical countries. The prevalence of Leptospira
    spp. in humans, cattle and dogs is unknown in Bhutan. Therefore, we sought to
    find out whether humans, cattle or dogs had been infected in the past with leptospires
    by measuring antibodies in the serum. We therefore collected blood from 864 humans
    ≥13 years of age, 130 bovines and 84 dogs from different rural and urban areas
    in Bhutan and tested the serum for antibodies specific for leptospires with a
    screening of enzyme-linked immunosorbent assays (ELISA) and a confirmatory microscopic
    agglutination test (MAT). In humans, 17.6% were seropositive by ELISA and 1.6%
    by MAT. The seropositivity was stronger in bovines (36.9%) and dogs (47.6%). “Having
    had a fever recently” (OR 5.2, p = 0.004), “working for the military” (OR 26.6,
    p = 0.028) and “being unemployed” (OR 12.9, p = 0.041) (reference category = housemaker)
    were statistically significantly associated with seropositivity when controlled
    for the effects of other risk factors. However, due to the small number of positive
    test results, the findings on risk factors should be interpreted with caution.
    Based on the serogroups found in the three species, dogs could be a source of
    infection for humans, or dogs and humans are exposed to the same environmental
    risk factors Clinical leptospirosis in humans and domestic animals should be investigated
    by testing blood and urine for the presence of leptospires by molecular methods
    (qPCR).
article_number: '308'
author:
- first_name: Anou
  full_name: Dreyfus, Anou
  last_name: Dreyfus
- first_name: Marie-Thérèse
  full_name: Ruf, Marie-Thérèse
  last_name: Ruf
- first_name: Anne
  full_name: Mayer-Scholl, Anne
  last_name: Mayer-Scholl
- first_name: Theresa
  full_name: Zitzl, Theresa
  last_name: Zitzl
- first_name: Nadine
  full_name: Loosli, Nadine
  last_name: Loosli
- first_name: Nadja Seyhan
  full_name: Bier, Nadja Seyhan
  last_name: Bier
- first_name: Stephanie
  full_name: Hiereth, Stephanie
  last_name: Hiereth
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Sven
  full_name: Poppert, Sven
  last_name: Poppert
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: John
  full_name: Stenos, John
  last_name: Stenos
- first_name: Tshokey
  full_name: Tshokey, Tshokey
  last_name: Tshokey
citation:
  ama: Dreyfus A, Ruf MT, Mayer-Scholl A, et al. Exposure to Leptospira spp. and Associated
    Risk Factors in the Human, Cattle and Dog Populations in Bhutan. <i>Pathogens</i>.
    2021;10(3). doi:<a href="https://doi.org/10.3390/pathogens10030308">10.3390/pathogens10030308</a>
  apa: Dreyfus, A., Ruf, M.-T., Mayer-Scholl, A., Zitzl, T., Loosli, N., Bier, N.
    S., Hiereth, S., Ulrich, S., Poppert, S., Straubinger, R. K., Stenos, J., &#38;
    Tshokey, T. (2021). Exposure to Leptospira spp. and Associated Risk Factors in
    the Human, Cattle and Dog Populations in Bhutan. <i>Pathogens</i>, <i>10</i>(3),
    Article 308. <a href="https://doi.org/10.3390/pathogens10030308">https://doi.org/10.3390/pathogens10030308</a>
  bjps: <b>Dreyfus A <i>et al.</i></b> (2021) Exposure to Leptospira Spp. and Associated
    Risk Factors in the Human, Cattle and Dog Populations in Bhutan. <i>Pathogens</i>
    <b>10</b>.
  chicago: Dreyfus, Anou, Marie-Thérèse Ruf, Anne Mayer-Scholl, Theresa Zitzl, Nadine
    Loosli, Nadja Seyhan Bier, Stephanie Hiereth, et al. “Exposure to Leptospira Spp.
    and Associated Risk Factors in the Human, Cattle and Dog Populations in Bhutan.”
    <i>Pathogens</i> 10, no. 3 (2021). <a href="https://doi.org/10.3390/pathogens10030308">https://doi.org/10.3390/pathogens10030308</a>.
  chicago-de: Dreyfus, Anou, Marie-Thérèse Ruf, Anne Mayer-Scholl, Theresa Zitzl,
    Nadine Loosli, Nadja Seyhan Bier, Stephanie Hiereth, u. a. 2021. Exposure to Leptospira
    spp. and Associated Risk Factors in the Human, Cattle and Dog Populations in Bhutan.
    <i>Pathogens</i> 10, Nr. 3. doi:<a href="https://doi.org/10.3390/pathogens10030308">10.3390/pathogens10030308</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Dreyfus,
    Anou</span> ; <span style="font-variant:small-caps;">Ruf, Marie-Thérèse</span>
    ; <span style="font-variant:small-caps;">Mayer-Scholl, Anne</span> ; <span style="font-variant:small-caps;">Zitzl,
    Theresa</span> ; <span style="font-variant:small-caps;">Loosli, Nadine</span>
    ; <span style="font-variant:small-caps;">Bier, Nadja Seyhan</span> ; <span style="font-variant:small-caps;">Hiereth,
    Stephanie</span> ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>
    ; u. a.</span>: Exposure to Leptospira spp. and Associated Risk Factors in the
    Human, Cattle and Dog Populations in Bhutan. In: <i>Pathogens</i> Bd. 10. Basel,
    MDPI (2021), Nr. 3'
  havard: A. Dreyfus, M.-T. Ruf, A. Mayer-Scholl, T. Zitzl, N. Loosli, N.S. Bier,
    S. Hiereth, S. Ulrich, S. Poppert, R.K. Straubinger, J. Stenos, T. Tshokey, Exposure
    to Leptospira spp. and Associated Risk Factors in the Human, Cattle and Dog Populations
    in Bhutan, Pathogens. 10 (2021).
  ieee: 'A. Dreyfus <i>et al.</i>, “Exposure to Leptospira spp. and Associated Risk
    Factors in the Human, Cattle and Dog Populations in Bhutan,” <i>Pathogens</i>,
    vol. 10, no. 3, Art. no. 308, 2021, doi: <a href="https://doi.org/10.3390/pathogens10030308">10.3390/pathogens10030308</a>.'
  mla: Dreyfus, Anou, et al. “Exposure to Leptospira Spp. and Associated Risk Factors
    in the Human, Cattle and Dog Populations in Bhutan.” <i>Pathogens</i>, vol. 10,
    no. 3, 308, 2021, <a href="https://doi.org/10.3390/pathogens10030308">https://doi.org/10.3390/pathogens10030308</a>.
  short: A. Dreyfus, M.-T. Ruf, A. Mayer-Scholl, T. Zitzl, N. Loosli, N.S. Bier, S.
    Hiereth, S. Ulrich, S. Poppert, R.K. Straubinger, J. Stenos, T. Tshokey, Pathogens
    10 (2021).
  ufg: '<b>Dreyfus, Anou u. a.</b>: Exposure to Leptospira spp. and Associated Risk
    Factors in the Human, Cattle and Dog Populations in Bhutan, in: <i>Pathogens</i>
    10 (2021), H. 3.'
  van: Dreyfus A, Ruf MT, Mayer-Scholl A, Zitzl T, Loosli N, Bier NS, et al. Exposure
    to Leptospira spp. and Associated Risk Factors in the Human, Cattle and Dog Populations
    in Bhutan. Pathogens. 2021;10(3).
date_created: 2025-06-15T09:59:24Z
date_updated: 2025-06-16T12:10:19Z
department:
- _id: DEP4010
doi: 10.3390/pathogens10030308
extern: '1'
intvolume: '        10'
issue: '3'
keyword:
- leptospirosis
- microscopic agglutination test (MAT)
- seroprevalence
- cattle
- yak
- dog
- one health
- Bhutan
language:
- iso: eng
place: Basel
publication: Pathogens
publication_identifier:
  eissn:
  - 2076-0817
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Exposure to Leptospira spp. and Associated Risk Factors in the Human, Cattle
  and Dog Populations in Bhutan
type: scientific_journal_article
user_id: '83781'
volume: 10
year: '2021'
...
---
_id: '12952'
abstract:
- lang: eng
  text: 'Straw is the main by-product of grain production, used as bedding material
    and animal feed. If produced or stored under adverse hygienic conditions, straw
    is prone to the growth of filamentous fungi. Some of them, e.g. Aspergillus, Fusarium
    and Stachybotrys spp. are well-known mycotoxin producers. Since studies on mycotoxins
    in straw are scarce, 192 straw samples (wheat n = 80; barley n = 79; triticale
    n = 12; oat n = 11; rye n = 12) were collected across Germany within the German
    official feed surveillance and screened for the presence of 21 mycotoxins. The
    following mycotoxins (positive samples for at least one mycotoxin n = 184) were
    detected: zearalenone (n = 86, 6.0–785 μg/kg), nivalenol (n = 51, 30–2,600 μg/kg),
    deoxynivalenol (n = 156, 20–24,000 μg/kg), 15-acetyl-deoxynivalenol (n = 34, 20–2,400
    μg/kg), 3-acetyl-deoxynivalenol (n = 16, 40–340 μg/kg), scirpentriol (n = 14,
    40–680 μg/kg), T-2 toxin (n = 67, 10–250 μg/kg), HT-2 toxin (n = 92, 20–800 μg/kg),
    T-2 tetraol (n = 13, 70–480 μg/kg). 15-monoacetoxyscirpenol (30 μg/kg) and T-2
    triol (60 μg/kg) were only detected in one barley sample. Macrocyclic trichothecenes
    (satratoxin G, F, roridin E, and verrucarin J) were also found in only one barley
    sample (quantified as roridin A equivalent: total 183 μg/kg). The occurrence of
    stachybotrylactam was monitored for the first time in four samples (n = 4, 0.96–7.4
    μg/kg). Fusarenon-X, 4,15-diacetoxyscirpenol, neosolaniol, satratoxin H and roridin-L2
    were not detectable in the samples. The results indicate a non-negligible contribution
    of straw to oral and possibly inhalation exposure to mycotoxins of animals or
    humans handling contaminated straw.'
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Barbara
  full_name: Biermaier, Barbara
  last_name: Biermaier
- first_name: Eunike
  full_name: Bahlinger, Eunike
  last_name: Bahlinger
- first_name: Magdalena
  full_name: Twarużek, Magdalena
  last_name: Twarużek
- first_name: Sarah
  full_name: Asmussen, Sarah
  last_name: Asmussen
- first_name: Margit
  full_name: Schollenberger, Margit
  last_name: Schollenberger
- first_name: Hana
  full_name: Valenta, Hana
  last_name: Valenta
- first_name: Frank
  full_name: Ebel, Frank
  last_name: Ebel
- first_name: Sven
  full_name: Dänicke, Sven
  last_name: Dänicke
citation:
  ama: Ulrich S, Gottschalk C, Biermaier B, et al. Occurrence of type A, B and D trichothecenes,
    zearalenone and stachybotrylactam in straw. <i>Archives of animal nutrition =
    Archiv für Tierernährung</i>. 2021;75(2):105-120. doi:<a href="https://doi.org/10.1080/1745039x.2021.1877075">10.1080/1745039x.2021.1877075</a>
  apa: Ulrich, S., Gottschalk, C., Biermaier, B., Bahlinger, E., Twarużek, M., Asmussen,
    S., Schollenberger, M., Valenta, H., Ebel, F., &#38; Dänicke, S. (2021). Occurrence
    of type A, B and D trichothecenes, zearalenone and stachybotrylactam in straw.
    <i>Archives of Animal Nutrition = Archiv Für Tierernährung</i>, <i>75</i>(2),
    105–120. <a href="https://doi.org/10.1080/1745039x.2021.1877075">https://doi.org/10.1080/1745039x.2021.1877075</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2021) Occurrence of Type A, B and D Trichothecenes,
    Zearalenone and Stachybotrylactam in Straw. <i>Archives of animal nutrition =
    Archiv für Tierernährung</i> <b>75</b>, 105–120.
  chicago: 'Ulrich, Sebastian, Christoph Gottschalk, Barbara Biermaier, Eunike Bahlinger,
    Magdalena Twarużek, Sarah Asmussen, Margit Schollenberger, Hana Valenta, Frank
    Ebel, and Sven Dänicke. “Occurrence of Type A, B and D Trichothecenes, Zearalenone
    and Stachybotrylactam in Straw.” <i>Archives of Animal Nutrition = Archiv Für
    Tierernährung</i> 75, no. 2 (2021): 105–20. <a href="https://doi.org/10.1080/1745039x.2021.1877075">https://doi.org/10.1080/1745039x.2021.1877075</a>.'
  chicago-de: 'Ulrich, Sebastian, Christoph Gottschalk, Barbara Biermaier, Eunike
    Bahlinger, Magdalena Twarużek, Sarah Asmussen, Margit Schollenberger, Hana Valenta,
    Frank Ebel und Sven Dänicke. 2021. Occurrence of type A, B and D trichothecenes,
    zearalenone and stachybotrylactam in straw. <i>Archives of animal nutrition =
    Archiv für Tierernährung</i> 75, Nr. 2: 105–120. doi:<a href="https://doi.org/10.1080/1745039x.2021.1877075">10.1080/1745039x.2021.1877075</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Gottschalk, Christoph</span>
    ; <span style="font-variant:small-caps;">Biermaier, Barbara</span> ; <span style="font-variant:small-caps;">Bahlinger,
    Eunike</span> ; <span style="font-variant:small-caps;">Twarużek, Magdalena</span>
    ; <span style="font-variant:small-caps;">Asmussen, Sarah</span> ; <span style="font-variant:small-caps;">Schollenberger,
    Margit</span> ; <span style="font-variant:small-caps;">Valenta, Hana</span> ;
    u. a.</span>: Occurrence of type A, B and D trichothecenes, zearalenone and stachybotrylactam
    in straw. In: <i>Archives of animal nutrition = Archiv für Tierernährung</i> Bd.
    75. Abingdon , Taylor &#38; Francis  (2021), Nr. 2, S. 105–120'
  havard: S. Ulrich, C. Gottschalk, B. Biermaier, E. Bahlinger, M. Twarużek, S. Asmussen,
    M. Schollenberger, H. Valenta, F. Ebel, S. Dänicke, Occurrence of type A, B and
    D trichothecenes, zearalenone and stachybotrylactam in straw, Archives of Animal
    Nutrition = Archiv Für Tierernährung. 75 (2021) 105–120.
  ieee: 'S. Ulrich <i>et al.</i>, “Occurrence of type A, B and D trichothecenes, zearalenone
    and stachybotrylactam in straw,” <i>Archives of animal nutrition = Archiv für
    Tierernährung</i>, vol. 75, no. 2, pp. 105–120, 2021, doi: <a href="https://doi.org/10.1080/1745039x.2021.1877075">10.1080/1745039x.2021.1877075</a>.'
  mla: Ulrich, Sebastian, et al. “Occurrence of Type A, B and D Trichothecenes, Zearalenone
    and Stachybotrylactam in Straw.” <i>Archives of Animal Nutrition = Archiv Für
    Tierernährung</i>, vol. 75, no. 2, 2021, pp. 105–20, <a href="https://doi.org/10.1080/1745039x.2021.1877075">https://doi.org/10.1080/1745039x.2021.1877075</a>.
  short: S. Ulrich, C. Gottschalk, B. Biermaier, E. Bahlinger, M. Twarużek, S. Asmussen,
    M. Schollenberger, H. Valenta, F. Ebel, S. Dänicke, Archives of Animal Nutrition
    = Archiv Für Tierernährung 75 (2021) 105–120.
  ufg: '<b>Ulrich, Sebastian u. a.</b>: Occurrence of type A, B and D trichothecenes,
    zearalenone and stachybotrylactam in straw, in: <i>Archives of animal nutrition
    = Archiv für Tierernährung</i> 75 (2021), H. 2,  S. 105–120.'
  van: Ulrich S, Gottschalk C, Biermaier B, Bahlinger E, Twarużek M, Asmussen S, et
    al. Occurrence of type A, B and D trichothecenes, zearalenone and stachybotrylactam
    in straw. Archives of animal nutrition = Archiv für Tierernährung. 2021;75(2):105–20.
date_created: 2025-06-15T09:59:54Z
date_updated: 2025-06-16T12:25:24Z
department:
- _id: DEP4010
doi: 10.1080/1745039x.2021.1877075
extern: '1'
intvolume: '        75'
issue: '2'
keyword:
- Fusarium
- mycotoxins
- stachybotrylactam
- stachybotrys
- straw
- trichothecenes
- zearalenone
language:
- iso: eng
page: 105-120
place: 'Abingdon '
publication: Archives of animal nutrition = Archiv für Tierernährung
publication_identifier:
  eissn:
  - 1477-2817
  issn:
  - 1745-039X
publication_status: published
publisher: 'Taylor & Francis '
quality_controlled: '1'
status: public
title: Occurrence of type A, B and D trichothecenes, zearalenone and stachybotrylactam
  in straw
type: scientific_journal_article
user_id: '83781'
volume: 75
year: '2021'
...
---
_id: '12953'
abstract:
- lang: eng
  text: Starch gelatinization is a major determinant of carbohydrate digestibility
    and varies with diet processing. Laboratory rodent diets are often marketed as
    identical, but are sold in different forms, regardless of the markedly higher
    starch gelatinization in extruded than in pelleted diets. Our hypothesis was that
    this would impact energy and nutrient digestibility in mice fed pellets or extrudate,
    respectively. Trial 1 showed that feeding C57BL/6 mice a standard maintenance
    diet in extruded form results in a significantly higher digestibility of organic
    matter, energy, and carbohydrates than the identical diet in pelleted form. The
    replication of the experiment, however, revealed a variation between batches of
    the same pelleted diet regarding starch and total dietary fiber contents. Given
    the significant differences in diet digestibility and the potential impacts of
    digestibility on nutrient utilization, the intestinal microbiome, and intermediary
    metabolism, trials performed with differently processed diets are not comparable.
    This might partly explain failures to reproduce results, especially in gastrointestinal
    or microbiome research. Considering this impact on experimental animals, the degree
    of starch gelatinization should be declared in the diet information for laboratory
    animal diets. The differences between batches of laboratory animal diets as observed
    in the pellets are not acceptable.
article_number: '523'
author:
- first_name: Linda F.
  full_name: Böswald, Linda F.
  last_name: Böswald
- first_name: Jasmin
  full_name: Wenderlein, Jasmin
  last_name: Wenderlein
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Ellen
  full_name: Kienzle, Ellen
  last_name: Kienzle
citation:
  ama: Böswald LF, Wenderlein J, Straubinger RK, Ulrich S, Kienzle E. Processing Matters
    in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility.
    <i>Animals</i>. 2021;11(2). doi:<a href="https://doi.org/10.3390/ani11020523">10.3390/ani11020523</a>
  apa: Böswald, L. F., Wenderlein, J., Straubinger, R. K., Ulrich, S., &#38; Kienzle,
    E. (2021). Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy
    and Nutrient Digestibility. <i>Animals</i>, <i>11</i>(2), Article 523. <a href="https://doi.org/10.3390/ani11020523">https://doi.org/10.3390/ani11020523</a>
  bjps: <b>Böswald LF <i>et al.</i></b> (2021) Processing Matters in Nutrient-Matched
    Laboratory Diets for Mice—Energy and Nutrient Digestibility. <i>Animals</i> <b>11</b>.
  chicago: Böswald, Linda F., Jasmin Wenderlein, Reinhard K. Straubinger, Sebastian
    Ulrich, and Ellen Kienzle. “Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Energy and Nutrient Digestibility.” <i>Animals</i> 11, no. 2 (2021).
    <a href="https://doi.org/10.3390/ani11020523">https://doi.org/10.3390/ani11020523</a>.
  chicago-de: Böswald, Linda F., Jasmin Wenderlein, Reinhard K. Straubinger, Sebastian
    Ulrich und Ellen Kienzle. 2021. Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Energy and Nutrient Digestibility. <i>Animals</i> 11, Nr. 2. doi:<a
    href="https://doi.org/10.3390/ani11020523">10.3390/ani11020523</a>, .
  din1505-2-1: '<span style="font-variant:small-caps;">Böswald, Linda F.</span> ;
    <span style="font-variant:small-caps;">Wenderlein, Jasmin</span> ; <span style="font-variant:small-caps;">Straubinger,
    Reinhard K.</span> ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>
    ; <span style="font-variant:small-caps;">Kienzle, Ellen</span>: Processing Matters
    in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility.
    In: <i>Animals</i> Bd. 11. Basel, MDPI (2021), Nr. 2'
  havard: L.F. Böswald, J. Wenderlein, R.K. Straubinger, S. Ulrich, E. Kienzle, Processing
    Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility,
    Animals. 11 (2021).
  ieee: 'L. F. Böswald, J. Wenderlein, R. K. Straubinger, S. Ulrich, and E. Kienzle,
    “Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient
    Digestibility,” <i>Animals</i>, vol. 11, no. 2, Art. no. 523, 2021, doi: <a href="https://doi.org/10.3390/ani11020523">10.3390/ani11020523</a>.'
  mla: Böswald, Linda F., et al. “Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Energy and Nutrient Digestibility.” <i>Animals</i>, vol. 11, no.
    2, 523, 2021, <a href="https://doi.org/10.3390/ani11020523">https://doi.org/10.3390/ani11020523</a>.
  short: L.F. Böswald, J. Wenderlein, R.K. Straubinger, S. Ulrich, E. Kienzle, Animals
    11 (2021).
  ufg: '<b>Böswald, Linda F. u. a.</b>: Processing Matters in Nutrient-Matched Laboratory
    Diets for Mice—Energy and Nutrient Digestibility, in: <i>Animals</i> 11 (2021),
    H. 2.'
  van: Böswald LF, Wenderlein J, Straubinger RK, Ulrich S, Kienzle E. Processing Matters
    in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility.
    Animals. 2021;11(2).
date_created: 2025-06-15T10:00:16Z
date_updated: 2025-06-16T12:27:09Z
department:
- _id: DEP4010
doi: 10.3390/ani11020523
extern: '1'
intvolume: '        11'
issue: '2'
keyword:
- standardization
- carbohydrate digestibility
- feed processing
- starch gelatinization
- gut
language:
- iso: eng
place: Basel
publication: Animals
publication_identifier:
  eissn:
  - 2076-2615
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and
  Nutrient Digestibility
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2021'
...
---
_id: '12954'
abstract:
- lang: eng
  text: Stachybotrys (S.) chartarum is a cellulolytic mould with the ability to produce
    highly cytotoxic macrocyclic trichothecenes. Two chemotypes are defined according
    to their ability to produce either atranones or satratoxins. S. chartarum has
    been well known as the causative agent of the lethal disease stachybotryotoxicosis
    in horses. Further investigations revealed that this disease is strictly correlated
    with the presence of macrocyclic trichothecenes. Furthermore, their occurrence
    in water-damaged buildings has been linked to adverse health effects such as the
    sick building syndrome. As the chemotypes cannot be characterized via phenotypic
    criteria, different methods such as PCR, MALDI–TOF MS, LC–MS/MS, thin-layer chromatography
    and cytotoxicity assays have been used so far. Fourier-transform-infrared spectroscopy
    (FT-IR) is commonly used for the differentiation of bacteria and yeasts, but this
    technique is also applicable to filamentous fungi. Hence, this study aimed at
    evaluating to which extent a reliable differentiation of S. chartarum chemotypes
    A and S is possible. Besides, another objective was to verify if the recently
    introduced third genotype of S. chartarum can be identified. Therefore, 28 strains
    including the two chemotypes and the third genotype H were cultivated on malt
    extract agar (MEA) and potato dextrose agar in three biological replicates. Each
    sample was applied to FT-IR measurements on day 7, 14 and 21 of cultivation. In
    this study, we achieved a distinction of the chemotypes A and S via FT-IR spectroscopy
    after incubation for 7 days on MEA. In terms of genotype differentiation, the
    PCR detecting satratoxin- and atranone-gene clusters remained the only applicable
    method.
author:
- first_name: Julia
  full_name: Ekruth, Julia
  last_name: Ekruth
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
citation:
  ama: Ekruth J, Gottschalk C, Ulrich S, Gareis M, Schwaiger K. Differentiation of
    S. chartarum (Ehrenb.) S. Hughes Chemotypes A and S via FT-IR Spectroscopy. <i>Mycopathologia</i>.
    2021;185(6):993-1004. doi:<a href="https://doi.org/10.1007/s11046-020-00495-0">10.1007/s11046-020-00495-0</a>
  apa: Ekruth, J., Gottschalk, C., Ulrich, S., Gareis, M., &#38; Schwaiger, K. (2021).
    Differentiation of S. chartarum (Ehrenb.) S. Hughes Chemotypes A and S via FT-IR
    Spectroscopy. <i>Mycopathologia</i>, <i>185</i>(6), 993–1004. <a href="https://doi.org/10.1007/s11046-020-00495-0">https://doi.org/10.1007/s11046-020-00495-0</a>
  bjps: <b>Ekruth J <i>et al.</i></b> (2021) Differentiation of S. Chartarum (Ehrenb.)
    S. Hughes Chemotypes A and S via FT-IR Spectroscopy. <i>Mycopathologia</i> <b>185</b>,
    993–1004.
  chicago: 'Ekruth, Julia, Christoph Gottschalk, Sebastian Ulrich, Manfred Gareis,
    and Karin Schwaiger. “Differentiation of S. Chartarum (Ehrenb.) S. Hughes Chemotypes
    A and S via FT-IR Spectroscopy.” <i>Mycopathologia</i> 185, no. 6 (2021): 993–1004.
    <a href="https://doi.org/10.1007/s11046-020-00495-0">https://doi.org/10.1007/s11046-020-00495-0</a>.'
  chicago-de: 'Ekruth, Julia, Christoph Gottschalk, Sebastian Ulrich, Manfred Gareis
    und Karin Schwaiger. 2021. Differentiation of S. chartarum (Ehrenb.) S. Hughes
    Chemotypes A and S via FT-IR Spectroscopy. <i>Mycopathologia</i> 185, Nr. 6: 993–1004.
    doi:<a href="https://doi.org/10.1007/s11046-020-00495-0">10.1007/s11046-020-00495-0</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Ekruth, Julia</span> ; <span
    style="font-variant:small-caps;">Gottschalk, Christoph</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Gareis, Manfred</span>
    ; <span style="font-variant:small-caps;">Schwaiger, Karin</span>: Differentiation
    of S. chartarum (Ehrenb.) S. Hughes Chemotypes A and S via FT-IR Spectroscopy.
    In: <i>Mycopathologia</i> Bd. 185. Dordrecht, Springer  (2021), Nr. 6, S. 993–1004'
  havard: J. Ekruth, C. Gottschalk, S. Ulrich, M. Gareis, K. Schwaiger, Differentiation
    of S. chartarum (Ehrenb.) S. Hughes Chemotypes A and S via FT-IR Spectroscopy,
    Mycopathologia. 185 (2021) 993–1004.
  ieee: 'J. Ekruth, C. Gottschalk, S. Ulrich, M. Gareis, and K. Schwaiger, “Differentiation
    of S. chartarum (Ehrenb.) S. Hughes Chemotypes A and S via FT-IR Spectroscopy,”
    <i>Mycopathologia</i>, vol. 185, no. 6, pp. 993–1004, 2021, doi: <a href="https://doi.org/10.1007/s11046-020-00495-0">10.1007/s11046-020-00495-0</a>.'
  mla: Ekruth, Julia, et al. “Differentiation of S. Chartarum (Ehrenb.) S. Hughes
    Chemotypes A and S via FT-IR Spectroscopy.” <i>Mycopathologia</i>, vol. 185, no.
    6, 2021, pp. 993–1004, <a href="https://doi.org/10.1007/s11046-020-00495-0">https://doi.org/10.1007/s11046-020-00495-0</a>.
  short: J. Ekruth, C. Gottschalk, S. Ulrich, M. Gareis, K. Schwaiger, Mycopathologia
    185 (2021) 993–1004.
  ufg: '<b>Ekruth, Julia u. a.</b>: Differentiation of S. chartarum (Ehrenb.) S. Hughes
    Chemotypes A and S via FT-IR Spectroscopy, in: <i>Mycopathologia</i> 185 (2021),
    H. 6,  S. 993–1004.'
  van: Ekruth J, Gottschalk C, Ulrich S, Gareis M, Schwaiger K. Differentiation of
    S. chartarum (Ehrenb.) S. Hughes Chemotypes A and S via FT-IR Spectroscopy. Mycopathologia.
    2021;185(6):993–1004.
date_created: 2025-06-15T10:00:44Z
date_updated: 2025-06-16T13:56:54Z
department:
- _id: DEP4010
doi: 10.1007/s11046-020-00495-0
extern: '1'
intvolume: '       185'
issue: '6'
keyword:
- Aspergillus nidulans
- Fungal biology
- Gas chromatography
- Pseudomonas fluorescens
- Western Blot
- Bacillus subtilis
language:
- iso: eng
page: 993-1004
place: Dordrecht
publication: Mycopathologia
publication_identifier:
  eissn:
  - 1573-0832
  issn:
  - 0301-486X
publication_status: published
publisher: 'Springer '
status: public
title: Differentiation of S. chartarum (Ehrenb.) S. Hughes Chemotypes A and S via
  FT-IR Spectroscopy
type: scientific_journal_article
user_id: '83781'
volume: 185
year: '2021'
...
---
_id: '12958'
abstract:
- lang: eng
  text: Cytotoxic macrocyclic trichothecenes such as satratoxins are produced by chemotype
    S strains of Stachybotrys chartarum. Diseases such as stachybotryotoxicosis in
    animals and the sick building syndrome as a multifactorial disease complex in
    humans have been associated with this mold and its toxins. Less toxic non-chemotype
    S strains of S. chartarum are morphologically indistinguishable from chemotype
    S strains, which results in uncertainties in hazard characterization of isolates.
    To selectively identify macrocyclic trichothecene producing S. chartarum isolates,
    a set of sat14 gene-specific primers was designed and applied in a loop-mediated
    isothermal amplification (LAMP) assay using neutral red for visual signal detection.
    The assay was highly specific for S. chartarum strains of the macrocyclic trichothecene
    producing chemotype and showed no cross-reaction with non-macrocyclic trichothecene
    producing S. chartarum strains or 152 strains of 131 other fungal species. The
    assay’s detection limit was 0.635 pg/rxn (picogram per reaction) with a reaction
    time of 60 min. Its high specificity and sensitivity as well as the cost-saving
    properties make the new assay an interesting and powerful diagnostic tool for
    easy and rapid testing.
author:
- first_name: Johannes
  full_name: Köck, Johannes
  last_name: Köck
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
- first_name: Ludwig
  full_name: Niessen, Ludwig
  last_name: Niessen
citation:
  ama: 'Köck J, Gottschalk C, Ulrich S, Schwaiger K, Gareis M, Niessen L. Rapid and
    selective detection of macrocyclic trichothecene producing Stachybotrys chartarum
    strains by loop-mediated isothermal amplification (LAMP). <i>Analytical and bioanalytical
    chemistry : a merger of Fresenius’ journal of analytical chemistry and Analusis</i>.
    2021;413(19):4801-4813. doi:<a href="https://doi.org/10.1007/s00216-021-03436-y">10.1007/s00216-021-03436-y</a>'
  apa: 'Köck, J., Gottschalk, C., Ulrich, S., Schwaiger, K., Gareis, M., &#38; Niessen,
    L. (2021). Rapid and selective detection of macrocyclic trichothecene producing
    Stachybotrys chartarum strains by loop-mediated isothermal amplification (LAMP).
    <i>Analytical and Bioanalytical Chemistry : A Merger of Fresenius’ Journal of
    Analytical Chemistry and Analusis</i>, <i>413</i>(19), 4801–4813. <a href="https://doi.org/10.1007/s00216-021-03436-y">https://doi.org/10.1007/s00216-021-03436-y</a>'
  bjps: '<b>Köck J <i>et al.</i></b> (2021) Rapid and Selective Detection of Macrocyclic
    Trichothecene Producing Stachybotrys Chartarum Strains by Loop-Mediated Isothermal
    Amplification (LAMP). <i>Analytical and bioanalytical chemistry : a merger of
    Fresenius’ journal of analytical chemistry and Analusis</i> <b>413</b>, 4801–4813.'
  chicago: 'Köck, Johannes, Christoph Gottschalk, Sebastian Ulrich, Karin Schwaiger,
    Manfred Gareis, and Ludwig Niessen. “Rapid and Selective Detection of Macrocyclic
    Trichothecene Producing Stachybotrys Chartarum Strains by Loop-Mediated Isothermal
    Amplification (LAMP).” <i>Analytical and Bioanalytical Chemistry : A Merger of
    Fresenius’ Journal of Analytical Chemistry and Analusis</i> 413, no. 19 (2021):
    4801–13. <a href="https://doi.org/10.1007/s00216-021-03436-y">https://doi.org/10.1007/s00216-021-03436-y</a>.'
  chicago-de: 'Köck, Johannes, Christoph Gottschalk, Sebastian Ulrich, Karin Schwaiger,
    Manfred Gareis und Ludwig Niessen. 2021. Rapid and selective detection of macrocyclic
    trichothecene producing Stachybotrys chartarum strains by loop-mediated isothermal
    amplification (LAMP). <i>Analytical and bioanalytical chemistry : a merger of
    Fresenius’ journal of analytical chemistry and Analusis</i> 413, Nr. 19: 4801–4813.
    doi:<a href="https://doi.org/10.1007/s00216-021-03436-y">10.1007/s00216-021-03436-y</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Köck, Johannes</span> ; <span
    style="font-variant:small-caps;">Gottschalk, Christoph</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Schwaiger, Karin</span>
    ; <span style="font-variant:small-caps;">Gareis, Manfred</span> ; <span style="font-variant:small-caps;">Niessen,
    Ludwig</span>: Rapid and selective detection of macrocyclic trichothecene producing
    Stachybotrys chartarum strains by loop-mediated isothermal amplification (LAMP).
    In: <i>Analytical and bioanalytical chemistry : a merger of Fresenius’ journal
    of analytical chemistry and Analusis</i> Bd. 413. Berlin ; Heidelberg, Springer  (2021),
    Nr. 19, S. 4801–4813'
  havard: 'J. Köck, C. Gottschalk, S. Ulrich, K. Schwaiger, M. Gareis, L. Niessen,
    Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys
    chartarum strains by loop-mediated isothermal amplification (LAMP), Analytical
    and Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry
    and Analusis. 413 (2021) 4801–4813.'
  ieee: 'J. Köck, C. Gottschalk, S. Ulrich, K. Schwaiger, M. Gareis, and L. Niessen,
    “Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys
    chartarum strains by loop-mediated isothermal amplification (LAMP),” <i>Analytical
    and bioanalytical chemistry : a merger of Fresenius’ journal of analytical chemistry
    and Analusis</i>, vol. 413, no. 19, pp. 4801–4813, 2021, doi: <a href="https://doi.org/10.1007/s00216-021-03436-y">10.1007/s00216-021-03436-y</a>.'
  mla: 'Köck, Johannes, et al. “Rapid and Selective Detection of Macrocyclic Trichothecene
    Producing Stachybotrys Chartarum Strains by Loop-Mediated Isothermal Amplification
    (LAMP).” <i>Analytical and Bioanalytical Chemistry : A Merger of Fresenius’ Journal
    of Analytical Chemistry and Analusis</i>, vol. 413, no. 19, 2021, pp. 4801–13,
    <a href="https://doi.org/10.1007/s00216-021-03436-y">https://doi.org/10.1007/s00216-021-03436-y</a>.'
  short: 'J. Köck, C. Gottschalk, S. Ulrich, K. Schwaiger, M. Gareis, L. Niessen,
    Analytical and Bioanalytical Chemistry : A Merger of Fresenius’ Journal of Analytical
    Chemistry and Analusis 413 (2021) 4801–4813.'
  ufg: '<b>Köck, Johannes u. a.</b>: Rapid and selective detection of macrocyclic
    trichothecene producing Stachybotrys chartarum strains by loop-mediated isothermal
    amplification (LAMP), in: <i>Analytical and bioanalytical chemistry : a merger
    of Fresenius’ journal of analytical chemistry and Analusis</i> 413 (2021), H.
    19,  S. 4801–4813.'
  van: 'Köck J, Gottschalk C, Ulrich S, Schwaiger K, Gareis M, Niessen L. Rapid and
    selective detection of macrocyclic trichothecene producing Stachybotrys chartarum
    strains by loop-mediated isothermal amplification (LAMP). Analytical and bioanalytical
    chemistry : a merger of Fresenius’ journal of analytical chemistry and Analusis.
    2021;413(19):4801–13.'
date_created: 2025-06-15T10:06:17Z
date_updated: 2025-06-16T14:17:01Z
department:
- _id: DEP4010
doi: 10.1007/s00216-021-03436-y
extern: '1'
intvolume: '       413'
issue: '19'
keyword:
- Aspergillus nidulans
- Basidiomycetes
- Fungi
- Strigolactone
- Liquid chromatography
- Solid-phase microextraction
language:
- iso: eng
page: 4801-4813
place: Berlin ; Heidelberg
publication: 'Analytical and bioanalytical chemistry : a merger of Fresenius'' journal
  of analytical chemistry and Analusis'
publication_identifier:
  eissn:
  - 1618-2650
  issn:
  - 1618-2642
publication_status: published
publisher: 'Springer '
quality_controlled: '1'
status: public
title: Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys
  chartarum strains by loop-mediated isothermal amplification (LAMP)
type: scientific_journal_article
user_id: '83781'
volume: 413
year: '2021'
...
---
_id: '12961'
abstract:
- lang: ger
  text: "Zu den Zecken-übertragenen Erkrankungen beim Pferd in Deutschland zählen
    neben der Equinen Granulozytären Anaplasmose (EGA, verursacht durch Anaplasma
    phagocytophilum, Ap) auch die Equine Lyme-Borreliose (verursacht durch den Borrelia-burgdorf-eri-sensu-lato-Komplex),
    die Frühsommer-Meningoencephalitis (FSME-Virus) und die Equine Piroplasmose (Babesia
    caballi, Theileria equi). Die EGA ist nicht kontagiös, so dass in der Regel innerhalb
    eines Bestandes nur einzelne Pferde betroffen sind. Der Schweregrad der Erkrankung
    ist vom Alter des Pferdes und der Dauer der Erkrankung abhängig. Zumeist tritt
    Apathie und Fieber auf. Jüngere Pferde (< 4 Jahre) entwickeln meist nur mildere
    klinische Veränderungen als ältere Pferde. In den meisten Fällen weist die EGA
    bei jungen Pferden und vor allem in Endemiegebieten,\r\neinen subklinischen oder
    milden Verlauf auf. Als Erregerreservoir dienen vor allem kleine wildlebende Säuger
    wie z.B. Nagetiere. Die Diagnose der EGA basiert auf der epizootischen Anamnese
    (jahreszeitlich und regional typisches Auftreten, vorhandene Zeckenexposition)
    sowie klinischen und labordiagnostischen Befunden. Der direkte Erregernachweis
    erfolgt durch Teilgensequenzierung, direkten mikroskopischen Nachweis oder Kultivierung.
    Auch indirekte Erregernachweisverfahren zur Diagnose der EGA in Form serologischer
    Laboruntersuchungen (Morulae bzw. Einschlusskörperchen) stehen zur Verfügung.
    Dabei kommen in der Regel ELISAs und Immunfluoreszenztests zum Einsatz. Ein Anstieg
    der Antikörperspiegel um das vierfache Niveau, lässt eine sichere Diagnose zu.
    Spezifische Antikörper gegen Ap können ab dem 14. Tag post infectionem und bis
    zu zwei Jahre später nachgewiesen werden. Die EGA kann effektiv mit Antibiotika
    behandelt werden. Dadurch wird die Erkrankungsdauer signifikant verkürzt und die
    Schwere der Erkrankung gemindert. Da Ap ein intrazelluläres Pathogen ist, sind
    Tetrazykline die Antibiotika der Wahl (Oxytetrazyklin intravenös in einer Dosis
    von 7 mg/kg Körpergewicht einmal täglich über 5–7 Tage). Da bisher keine Impfung
    gegen die EGA zur Verfügung steht, sind die Prophylaxe-Maßnahmen auf die Verhinderung
    oder Minderung einer Zeckenexposition beschränkt."
author:
- first_name: Heidrun
  full_name: Gehlen, Heidrun
  last_name: Gehlen
- first_name: 'Katharina '
  full_name: 'Inerle, Katharina '
  last_name: Inerle
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Beatrice
  full_name: Lehmann, Beatrice
  last_name: Lehmann
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: Gehlen H, Inerle K, Ulrich S, Lehmann B, Straubinger RK. Anaplasmose beim Pferd
    – Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und Therapieverfahren
    H. Gehlen et al. Pferdeheilkunde – Equine Medicine 37 (2021)25 Pferdeheilkunde
    – Equine Medicine 37 (2021) 1 (Januar/Februar) 25–33 Anaplasmose beim Pferd –
    Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und Therapie- verfahren
    sowie möglicher Präventionsmaßnahmen. <i>Pferdeheilkunde Equine Medicine</i>.
    2021;37(1):25-33. doi:<a href="https://doi.org/10.21836/PEM20210104">10.21836/PEM20210104</a>
  apa: Gehlen, H., Inerle, K., Ulrich, S., Lehmann, B., &#38; Straubinger, R. K. (2021).
    Anaplasmose beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller
    Diagnose- und Therapieverfahren H. Gehlen et al. Pferdeheilkunde – Equine Medicine
    37 (2021)25 Pferdeheilkunde – Equine Medicine 37 (2021) 1 (Januar/Februar) 25–33
    Anaplasmose beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller
    Diagnose- und Therapie- verfahren sowie möglicher Präventionsmaßnahmen. <i>Pferdeheilkunde
    Equine Medicine</i>, <i>37</i>(1), 25–33. <a href="https://doi.org/10.21836/PEM20210104">https://doi.org/10.21836/PEM20210104</a>
  bjps: <b>Gehlen H <i>et al.</i></b> (2021) Anaplasmose beim Pferd – Ein Literaturreview
    unter Berücksichtigung aktueller Diagnose- und Therapieverfahren H. Gehlen et
    al. Pferdeheilkunde – Equine Medicine 37 (2021)25 Pferdeheilkunde – Equine Medicine
    37 (2021) 1 (Januar/Februar) 25–33 Anaplasmose beim Pferd – Ein Literaturreview
    unter Berücksichtigung aktueller Diagnose- und Therapie- verfahren sowie möglicher
    Präventionsmaßnahmen. <i>Pferdeheilkunde Equine Medicine</i> <b>37</b>, 25–33.
  chicago: 'Gehlen, Heidrun, Katharina  Inerle, Sebastian Ulrich, Beatrice Lehmann,
    and Reinhard K. Straubinger. “Anaplasmose beim Pferd – Ein Literaturreview unter
    Berücksichtigung aktueller Diagnose- und Therapieverfahren H. Gehlen et al. Pferdeheilkunde
    – Equine Medicine 37 (2021)25 Pferdeheilkunde – Equine Medicine 37 (2021) 1 (Januar/Februar)
    25–33 Anaplasmose beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller
    Diagnose- und Therapie- verfahren sowie möglicher Präventionsmaßnahmen.” <i>Pferdeheilkunde
    Equine Medicine</i> 37, no. 1 (2021): 25–33. <a href="https://doi.org/10.21836/PEM20210104">https://doi.org/10.21836/PEM20210104</a>.'
  chicago-de: 'Gehlen, Heidrun, Katharina  Inerle, Sebastian Ulrich, Beatrice Lehmann
    und Reinhard K. Straubinger. 2021. Anaplasmose beim Pferd – Ein Literaturreview
    unter Berücksichtigung aktueller Diagnose- und Therapieverfahren H. Gehlen et
    al. Pferdeheilkunde – Equine Medicine 37 (2021)25 Pferdeheilkunde – Equine Medicine
    37 (2021) 1 (Januar/Februar) 25–33 Anaplasmose beim Pferd – Ein Literaturreview
    unter Berücksichtigung aktueller Diagnose- und Therapie- verfahren sowie möglicher
    Präventionsmaßnahmen. <i>Pferdeheilkunde Equine Medicine</i> 37, Nr. 1: 25–33.
    doi:<a href="https://doi.org/10.21836/PEM20210104">10.21836/PEM20210104</a>, .'
  din1505-2-1: '<span style="font-variant:small-caps;">Gehlen, Heidrun</span> ; <span
    style="font-variant:small-caps;">Inerle, Katharina </span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Lehmann, Beatrice</span>
    ; <span style="font-variant:small-caps;">Straubinger, Reinhard K.</span>: Anaplasmose
    beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und
    Therapieverfahren H. Gehlen et al. Pferdeheilkunde – Equine Medicine 37 (2021)25
    Pferdeheilkunde – Equine Medicine 37 (2021) 1 (Januar/Februar) 25–33 Anaplasmose
    beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und
    Therapie- verfahren sowie möglicher Präventionsmaßnahmen. In: <i>Pferdeheilkunde
    Equine Medicine</i> Bd. 37. Stuttgart, Hippiatrika GmbH (2021), Nr. 1, S. 25–33'
  havard: H. Gehlen, K. Inerle, S. Ulrich, B. Lehmann, R.K. Straubinger, Anaplasmose
    beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und
    Therapieverfahren H. Gehlen et al. Pferdeheilkunde – Equine Medicine 37 (2021)25
    Pferdeheilkunde – Equine Medicine 37 (2021) 1 (Januar/Februar) 25–33 Anaplasmose
    beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und
    Therapie- verfahren sowie möglicher Präventionsmaßnahmen, Pferdeheilkunde Equine
    Medicine. 37 (2021) 25–33.
  ieee: 'H. Gehlen, K. Inerle, S. Ulrich, B. Lehmann, and R. K. Straubinger, “Anaplasmose
    beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und
    Therapieverfahren H. Gehlen et al. Pferdeheilkunde – Equine Medicine 37 (2021)25
    Pferdeheilkunde – Equine Medicine 37 (2021) 1 (Januar/Februar) 25–33 Anaplasmose
    beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und
    Therapie- verfahren sowie möglicher Präventionsmaßnahmen,” <i>Pferdeheilkunde
    Equine Medicine</i>, vol. 37, no. 1, pp. 25–33, 2021, doi: <a href="https://doi.org/10.21836/PEM20210104">10.21836/PEM20210104</a>.'
  mla: Gehlen, Heidrun, et al. “Anaplasmose beim Pferd – Ein Literaturreview unter
    Berücksichtigung aktueller Diagnose- und Therapieverfahren H. Gehlen et al. Pferdeheilkunde
    – Equine Medicine 37 (2021)25 Pferdeheilkunde – Equine Medicine 37 (2021) 1 (Januar/Februar)
    25–33 Anaplasmose beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller
    Diagnose- und Therapie- verfahren sowie möglicher Präventionsmaßnahmen.” <i>Pferdeheilkunde
    Equine Medicine</i>, vol. 37, no. 1, 2021, pp. 25–33, <a href="https://doi.org/10.21836/PEM20210104">https://doi.org/10.21836/PEM20210104</a>.
  short: H. Gehlen, K. Inerle, S. Ulrich, B. Lehmann, R.K. Straubinger, Pferdeheilkunde
    Equine Medicine 37 (2021) 25–33.
  ufg: '<b>Gehlen, Heidrun u. a.</b>: Anaplasmose beim Pferd – Ein Literaturreview
    unter Berücksichtigung aktueller Diagnose- und Therapieverfahren H. Gehlen et
    al. Pferdeheilkunde – Equine Medicine 37 (2021)25 Pferdeheilkunde – Equine Medicine
    37 (2021) 1 (Januar/Februar) 25–33 Anaplasmose beim Pferd – Ein Literaturreview
    unter Berücksichtigung aktueller Diagnose- und Therapie- verfahren sowie möglicher
    Präventionsmaßnahmen, in: <i>Pferdeheilkunde Equine Medicine</i> 37 (2021), H.
    1,  S. 25–33.'
  van: Gehlen H, Inerle K, Ulrich S, Lehmann B, Straubinger RK. Anaplasmose beim Pferd
    – Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und Therapieverfahren
    H. Gehlen et al. Pferdeheilkunde – Equine Medicine 37 (2021)25 Pferdeheilkunde
    – Equine Medicine 37 (2021) 1 (Januar/Februar) 25–33 Anaplasmose beim Pferd –
    Ein Literaturreview unter Berücksichtigung aktueller Diagnose- und Therapie- verfahren
    sowie möglicher Präventionsmaßnahmen. Pferdeheilkunde Equine Medicine. 2021;37(1):25–33.
date_created: 2025-06-15T10:10:43Z
date_updated: 2025-08-15T08:19:27Z
department:
- _id: DEP4010
doi: 10.21836/PEM20210104
extern: '1'
intvolume: '        37'
issue: '1'
keyword:
- Pferd
- Anaplasmose
- Infektion
- Diagnostik
- Prävention
language:
- iso: ger
main_file_link:
- open_access: '1'
  url: https://pferdeheilkunde.de/files/20210104.pdf
oa: '1'
page: 25-33
place: Stuttgart
publication: Pferdeheilkunde Equine Medicine
publication_identifier:
  eissn:
  - 2943-1794
  issn:
  - 0177-7726
publication_status: published
publisher: Hippiatrika GmbH
status: public
title: Anaplasmose beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller
  Diagnose- und Therapieverfahren H. Gehlen et al. Pferdeheilkunde – Equine Medicine
  37 (2021)25 Pferdeheilkunde – Equine Medicine 37 (2021) 1 (Januar/Februar) 25–33
  Anaplasmose beim Pferd – Ein Literaturreview unter Berücksichtigung aktueller Diagnose-
  und Therapie- verfahren sowie möglicher Präventionsmaßnahmen
type: scientific_journal_article
user_id: '85847'
volume: 37
year: '2021'
...
---
_id: '12955'
abstract:
- lang: ger
  text: Ein 10 Monate alter, männlich intakter Rhodesian Ridgeback wurde wegen chronischen
    Dickdarmdurchfalls und Hämatochezie vorgestellt. Der Hund stammte aus Deutschland
    und hatte das Land nie verlassen. Die Laboruntersuchung des vorbehandelnden Tierarztes
    ergab neben einer Neutrophilie eine Hyperkaliämie und eine Hyponatriämie. Mit
    einem Serumbasalkortisolwert von 4,3 µg/dl konnte ein Hypoadrenokortizismus weitgehend
    ausgeschlossen werden. Eine vom Tierarzt durch geführte antibiotische Behandlung
    hatte keine Besserung bewirkt. Daher war der Hund mit Prednisolon behandelt worden.
    Unter 2-wöchiger Prednisolongabe kam es zu einer deutlichen Verstärkung des Durchfalls
    sowie einem Gewichtsverlust von 6 kg. Bei Vorstellung an der Medizinischen Kleintierklinik
    der LMU München war der Hund im Allgemeinbefinden mittelgradig reduziert, deutlich
    abgemagert, dehydriert, hypovolämisch und hatte eine rektale Körpertemperatur
    von 39,6 °C. Bei der sonografischen Untersuchung zeigte sich eine generalisiert
    verdickte Dickdarmwand und koloskopisch eine hochgradig ulzerativ veränderte Dickdarmschleimhaut.
    Histologischer Befund war eine ulzerative granulomatöse Kolitis. Durch die Periodic-Acid-Schiff-Reaktion
    ließen sich in den Schnitten der Dickdarmbioptate mikrobielle Strukturen darstellen,
    die für eine Algeninfektion diagnostisch waren. Die bei der mikrobiellen Untersuchung
    anzüchtbaren Prototheken wurden mittels MALDI-TOF-MS als Prototheca zopfii identifiziert.
    Zum Nachweis einer möglichen Immundefizienz wurden die Immunglobuline im Serum
    bestimmt. Die IgM-Konzentration war erniedrigt, während sich IgG- und IgA-Konzentration
    im Referenzbereich befanden. Aufgrund der Verschlechterung des Allgemeinbefindens,
    der vorsichtigen Prognose und der hohen Kosten eines Therapieversuchs wurde der
    Hund eine Woche später euthanasiert und der Tierkörper pathologisch untersucht.
    Histopathologisch wurden Prototheken auch in den abdominalen Lymphknoten, jedoch
    nicht in den Augen oder im zentralen Nervensystem identifiziert. Der Fall zeigt,
    dass eine Prototheken-Infektion auch bei Hunden aus Deutschland als Differenzialdiagnose
    für chronischen Dickdarmdurchfall in Betracht gezogen werden sollte, insbesondere
    bei Patienten mit ulzerativer granulomatöser Kolitis. Sie kann bei der histologischen
    Untersuchung ohne Spezialfärbung leicht übersehen werden.
- lang: eng
  text: A 10-month-old male Rhodesian Ridgeback was presented to the Clinic of Small
    Animal Medicine, LMU, Germany, with a 6-month history of chronic diarrhea and
    hematochezia. The dog lived in Germany and had never traveled abroad. Complete
    blood count and serum biochemistry performed by the referring veterinarian revealed
    neutrophilia, hyperkalemia, and hyponatremia, with a basal cortisol of 4.3 µg/dl,
    which excluded hypoadrenocorticism. Since antibiotic treatment had not resulted
    in any improvement, a 2 week course of prednisolone administration had been initiated,
    leading to a marked deterioration of intestinal signs and a significant weight
    loss of 6 kg. At the time of referral, the patient was markedly emaciated, dehydrated,
    hypovolemic and had a rectal temperature of 39.6 °C. Abdominal ultrasound showed
    a thickened and irregular colonic wall. On colonoscopy, an irregular colonic mucosa
    with ulcerations was observed. Histopathologic examination revealed an ulcerative
    granulomatous colitis, and on Periodic acid-Schiff reaction (PAS) numerous organisms
    consistent with Prototheca spp. were identified. Prototheca zopfii infection was
    confirmed by culture and MALDI-TOF MS. In order to test for an underlying immunodeficiency,
    immunoglobulin levels in serum were determined. IgM was decreased, while IgG and
    IgA levels were within the reference interval. Due to deterioration of general
    condition, grave prognosis and costs of a treatment trial, the patient was euthanized
    one week later, and necropsy was performed. Prototheca spp. were detected on histopathologic
    examination in the lymphnodes, however not in the eyes or the central nervous
    system. Protothecosis should be considered an differential diagnosis in dogs with
    chronic diarrhea and ulcerative granulomatous colitis even in dogs living in Germany.
    Histopathologic examination of colonic biopsies with special stains such as PAS
    is recommended in every dog with signs of chronic large bowel disease in order
    to avoid missing this rare infectious disease.
author:
- first_name: Vera
  full_name: Geisen, Vera
  last_name: Geisen
- first_name: Christian
  full_name: Mayer, Christian
  last_name: Mayer
- first_name: Julia
  full_name: Harrer, Julia
  last_name: Harrer
- first_name: Katrin
  full_name: Hartmann, Katrin
  last_name: Hartmann
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Stefan
  full_name: Unterer, Stefan
  last_name: Unterer
citation:
  ama: 'Geisen V, Mayer C, Harrer J, Hartmann K, Ulrich S, Unterer S. Ulzerative granulomatöse
    Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. <i>Tierärztliche
    Praxis Ausgabe K: Kleintiere / Heimtiere</i>. 2020;48(05):369-375. doi:<a href="https://doi.org/10.1055/a-1238-1554">10.1055/a-1238-1554</a>'
  apa: 'Geisen, V., Mayer, C., Harrer, J., Hartmann, K., Ulrich, S., &#38; Unterer,
    S. (2020). Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian
    Ridgeback in Deutschland. <i>Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere</i>,
    <i>48</i>(05), 369–375. <a href="https://doi.org/10.1055/a-1238-1554">https://doi.org/10.1055/a-1238-1554</a>'
  bjps: '<b>Geisen V <i>et al.</i></b> (2020) Ulzerative granulomatöse Kolitis durch
    Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. <i>Tierärztliche
    Praxis Ausgabe K: Kleintiere / Heimtiere</i> <b>48</b>, 369–375.'
  chicago: 'Geisen, Vera, Christian Mayer, Julia Harrer, Katrin Hartmann, Sebastian
    Ulrich, and Stefan Unterer. “Ulzerative granulomatöse Kolitis durch Prototheca
    spp. bei einem Rhodesian Ridgeback in Deutschland.” <i>Tierärztliche Praxis Ausgabe
    K: Kleintiere / Heimtiere</i> 48, no. 05 (2020): 369–75. <a href="https://doi.org/10.1055/a-1238-1554">https://doi.org/10.1055/a-1238-1554</a>.'
  chicago-de: 'Geisen, Vera, Christian Mayer, Julia Harrer, Katrin Hartmann, Sebastian
    Ulrich und Stefan Unterer. 2020. Ulzerative granulomatöse Kolitis durch Prototheca
    spp. bei einem Rhodesian Ridgeback in Deutschland. <i>Tierärztliche Praxis Ausgabe
    K: Kleintiere / Heimtiere</i> 48, Nr. 05: 369–375. doi:<a href="https://doi.org/10.1055/a-1238-1554">10.1055/a-1238-1554</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Geisen, Vera</span> ; <span
    style="font-variant:small-caps;">Mayer, Christian</span> ; <span style="font-variant:small-caps;">Harrer,
    Julia</span> ; <span style="font-variant:small-caps;">Hartmann, Katrin</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Unterer,
    Stefan</span>: Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem
    Rhodesian Ridgeback in Deutschland. In: <i>Tierärztliche Praxis Ausgabe K: Kleintiere
    / Heimtiere</i> Bd. 48. Stuttgart, Thieme  (2020), Nr. 05, S. 369–375'
  havard: 'V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, S. Unterer, Ulzerative
    granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland,
    Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere. 48 (2020) 369–375.'
  ieee: 'V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, and S. Unterer, “Ulzerative
    granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland,”
    <i>Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere</i>, vol. 48, no. 05,
    pp. 369–375, 2020, doi: <a href="https://doi.org/10.1055/a-1238-1554">10.1055/a-1238-1554</a>.'
  mla: 'Geisen, Vera, et al. “Ulzerative granulomatöse Kolitis durch Prototheca spp.
    bei einem Rhodesian Ridgeback in Deutschland.” <i>Tierärztliche Praxis Ausgabe
    K: Kleintiere / Heimtiere</i>, vol. 48, no. 05, 2020, pp. 369–75, <a href="https://doi.org/10.1055/a-1238-1554">https://doi.org/10.1055/a-1238-1554</a>.'
  short: 'V. Geisen, C. Mayer, J. Harrer, K. Hartmann, S. Ulrich, S. Unterer, Tierärztliche
    Praxis Ausgabe K: Kleintiere / Heimtiere 48 (2020) 369–375.'
  ufg: '<b>Geisen, Vera u. a.</b>: Ulzerative granulomatöse Kolitis durch Prototheca
    spp. bei einem Rhodesian Ridgeback in Deutschland, in: <i>Tierärztliche Praxis
    Ausgabe K: Kleintiere / Heimtiere</i> 48 (2020), H. 05,  S. 369–375.'
  van: 'Geisen V, Mayer C, Harrer J, Hartmann K, Ulrich S, Unterer S. Ulzerative granulomatöse
    Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland. Tierärztliche
    Praxis Ausgabe K: Kleintiere / Heimtiere. 2020;48(05):369–75.'
date_created: 2025-06-15T10:01:12Z
date_updated: 2025-06-18T12:24:09Z
department:
- _id: DEP4010
doi: 10.1055/a-1238-1554
extern: '1'
intvolume: '        48'
issue: '05'
keyword:
- Hund - Algeninfektion - chronischer Durchfall - Protothekose - Prototheca zopfii
language:
- iso: ger
page: 369-375
place: Stuttgart
publication: 'Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere'
publication_identifier:
  eissn:
  - 2567-5842
  issn:
  - 1434-1239
publication_status: published
publisher: 'Thieme '
status: public
title: Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian
  Ridgeback in Deutschland
type: scientific_journal_article
user_id: '83781'
volume: 48
year: '2020'
...
---
_id: '12956'
abstract:
- lang: eng
  text: Stachybotrys (S.) chartarum had been linked to severe health problems in humans
    and animals, which occur after exposure to the toxic secondary metabolites of
    this mold. S. chartarum had been isolated from different environmental sources,
    ranging from culinary herbs and improperly stored fodder to damp building materials.
    To access the pathogenic potential of isolates, it is essential to analyze them
    under defined conditions that allow for the production of their toxic metabolites.
    All Stachybotrys species are assumed to produce the immunosuppressive phenylspirodrimanes,
    but the highly cytotoxic macrocyclic trichothecenes are exclusively generated
    by the genotype S of S. chartarum. In this study, we have analyzed four genotype
    S strains initially isolated from three different habitats. We grew them on five
    commonly used media (malt-extract-agar, glucose-yeast-peptone-agar, potato-dextrose-agar,
    cellulose-agar, Sabouraud-dextrose-agar) to identify conditions that promote mycotoxin
    production. Using LC-MS/MS, we have quantified stachybotrylactam and all S-type
    specific macrocyclic trichothecenes (satratoxin G, H, F, roridin E, L-2, verrucarin
    J). All five media supported a comparable fungal growth and sporulation at 25
    °C in the dark. The highest concentrations of macrocyclic trichothecenes were
    detected on potato-dextrose-agar or cellulose-agar. Malt-extract-agar let to an
    intermediate and glucose-yeast-peptone-agar and Sabouraud-dextrose-agar to a poor
    mycotoxin production. These data demonstrate that the mycotoxin production clearly
    depends on the composition of the respective medium. Our findings provide a starting
    point for further studies in order to identify individual components that either
    support or repress the production of mycotoxins in S. chartarum.
article_number: '159'
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Cornelius
  full_name: Schäfer, Cornelius
  last_name: Schäfer
citation:
  ama: Ulrich S, Schäfer C. Toxin Production by Stachybotrys chartarum Genotype S
    on Different Culture Media. <i>Journal of Fungi</i>. 2020;6(3). doi:<a href="https://doi.org/10.3390/jof6030159">10.3390/jof6030159</a>
  apa: Ulrich, S., &#38; Schäfer, C. (2020). Toxin Production by Stachybotrys chartarum
    Genotype S on Different Culture Media. <i>Journal of Fungi</i>, <i>6</i>(3), Article
    159. <a href="https://doi.org/10.3390/jof6030159">https://doi.org/10.3390/jof6030159</a>
  bjps: <b>Ulrich S and Schäfer C</b> (2020) Toxin Production by Stachybotrys Chartarum
    Genotype S on Different Culture Media. <i>Journal of Fungi</i> <b>6</b>.
  chicago: Ulrich, Sebastian, and Cornelius Schäfer. “Toxin Production by Stachybotrys
    Chartarum Genotype S on Different Culture Media.” <i>Journal of Fungi</i> 6, no.
    3 (2020). <a href="https://doi.org/10.3390/jof6030159">https://doi.org/10.3390/jof6030159</a>.
  chicago-de: Ulrich, Sebastian und Cornelius Schäfer. 2020. Toxin Production by Stachybotrys
    chartarum Genotype S on Different Culture Media. <i>Journal of Fungi</i> 6, Nr.
    3. doi:<a href="https://doi.org/10.3390/jof6030159">10.3390/jof6030159</a>, .
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Schäfer, Cornelius</span>: Toxin Production
    by Stachybotrys chartarum Genotype S on Different Culture Media. In: <i>Journal
    of Fungi</i> Bd. 6. Basel, MDPI  (2020), Nr. 3'
  havard: S. Ulrich, C. Schäfer, Toxin Production by Stachybotrys chartarum Genotype
    S on Different Culture Media, Journal of Fungi. 6 (2020).
  ieee: 'S. Ulrich and C. Schäfer, “Toxin Production by Stachybotrys chartarum Genotype
    S on Different Culture Media,” <i>Journal of Fungi</i>, vol. 6, no. 3, Art. no.
    159, 2020, doi: <a href="https://doi.org/10.3390/jof6030159">10.3390/jof6030159</a>.'
  mla: Ulrich, Sebastian, and Cornelius Schäfer. “Toxin Production by Stachybotrys
    Chartarum Genotype S on Different Culture Media.” <i>Journal of Fungi</i>, vol.
    6, no. 3, 159, 2020, <a href="https://doi.org/10.3390/jof6030159">https://doi.org/10.3390/jof6030159</a>.
  short: S. Ulrich, C. Schäfer, Journal of Fungi 6 (2020).
  ufg: '<b>Ulrich, Sebastian/Schäfer, Cornelius</b>: Toxin Production by Stachybotrys
    chartarum Genotype S on Different Culture Media, in: <i>Journal of Fungi</i> 6
    (2020), H. 3.'
  van: Ulrich S, Schäfer C. Toxin Production by Stachybotrys chartarum Genotype S
    on Different Culture Media. Journal of Fungi. 2020;6(3).
date_created: 2025-06-15T10:01:42Z
date_updated: 2025-06-16T13:59:56Z
department:
- _id: DEP4010
doi: 10.3390/jof6030159
extern: '1'
intvolume: '         6'
issue: '3'
keyword:
- Stachybotrys
- genotype
- macrocyclic trichothecenes
- stachybotrylactam
language:
- iso: eng
place: Basel
publication: Journal of Fungi
publication_identifier:
  eissn:
  - 2309-608X
publication_status: published
publisher: 'MDPI '
quality_controlled: '1'
status: public
title: Toxin Production by Stachybotrys chartarum Genotype S on Different Culture
  Media
type: scientific_journal_article
user_id: '83781'
volume: 6
year: '2020'
...
---
_id: '12962'
abstract:
- lang: eng
  text: "Background\r\nBorrelia burgdorferi is a tick-borne spirochete that causes
    Lyme borreliosis (LB). After an initial tick bite, it spreads from the deposition
    site in the dermis to distant tissues of the host. It is generally believed that
    this spirochete disseminates via the hematogenous route. Borrelia persica causes
    relapsing fever and is able to replicate in the blood stream. Currently the exact
    dissemination pathway of LB pathogens in the host is not known and controversially
    discussed.\r\nMethods\r\nIn this study, we established a strict intravenous infection
    murine model using host-adapted spirochetes. Survival capacity and infectivity
    of host-adapted B. burgdorferi sensu stricto (Bbss) were compared to those of
    B. persica (Bp) after either intradermal (ID) injection into the dorsal skin of
    immunocompetent mice or strict intravenous (IV) inoculation via the jugular vein.
    By in vitro culture and PCR, viable spirochetes and their DNA load in peripheral
    blood were periodically monitored during a 49/50-day course post-injection, as
    well as in various tissue samples collected at day 49/50. Specific antibodies
    in individual plasma/serum samples were detected with serological methods.\r\nResults\r\nRegardless
    of ID or IV injection, DNA of Bp was present in blood samples up to day 24 post-challenge,
    while no Bbss was detectable in the blood circulation during the complete observation
    period. In contrast to the brain tropism of Bp, Bbss spirochetes were found in
    ear, skin, joint, bladder, and heart tissue samples of only ID-inoculated mice.
    All tested tissues collected from IV-challenged mice were negative for traces
    of Bbss. ELISA testing of serum samples showed that Bp induced gradually increasing
    antibody levels after ID or IV inoculation, while Bbss did so only after ID injection
    but not after IV inoculation.\r\nConclusions\r\nThis study allows us to draw the
    following conclusions: (i) Bp survives in the blood and disseminates to the host’s
    brain via the hematogenous route; and (ii) Bbss, in contrast, is cleared rapidly
    from the blood stream and is a tissue-bound spirochete."
article_number: '191'
author:
- first_name: Liucun
  full_name: Liang, Liucun
  last_name: Liang
- first_name: Jinyong
  full_name: Wang, Jinyong
  last_name: Wang
- first_name: Lucas
  full_name: Schorter, Lucas
  last_name: Schorter
- first_name: Thu Phong
  full_name: Nguyen Trong, Thu Phong
  last_name: Nguyen Trong
- first_name: Shari
  full_name: Fell, Shari
  last_name: Fell
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
citation:
  ama: Liang L, Wang J, Schorter L, et al. Rapid clearance of Borrelia burgdorferi
    from the blood circulation. <i>Parasites &#38; vectors</i>. 2020;13(1). doi:<a
    href="https://doi.org/10.1186/s13071-020-04060-y">10.1186/s13071-020-04060-y</a>
  apa: Liang, L., Wang, J., Schorter, L., Nguyen Trong, T. P., Fell, S., Ulrich, S.,
    &#38; Straubinger, R. K. (2020). Rapid clearance of Borrelia burgdorferi from
    the blood circulation. <i>Parasites &#38; Vectors</i>, <i>13</i>(1), Article 191.
    <a href="https://doi.org/10.1186/s13071-020-04060-y">https://doi.org/10.1186/s13071-020-04060-y</a>
  bjps: <b>Liang L <i>et al.</i></b> (2020) Rapid Clearance of Borrelia Burgdorferi
    from the Blood Circulation. <i>Parasites &#38; vectors</i> <b>13</b>.
  chicago: Liang, Liucun, Jinyong Wang, Lucas Schorter, Thu Phong Nguyen Trong, Shari
    Fell, Sebastian Ulrich, and Reinhard K. Straubinger. “Rapid Clearance of Borrelia
    Burgdorferi from the Blood Circulation.” <i>Parasites &#38; Vectors</i> 13, no.
    1 (2020). <a href="https://doi.org/10.1186/s13071-020-04060-y">https://doi.org/10.1186/s13071-020-04060-y</a>.
  chicago-de: Liang, Liucun, Jinyong Wang, Lucas Schorter, Thu Phong Nguyen Trong,
    Shari Fell, Sebastian Ulrich und Reinhard K. Straubinger. 2020. Rapid clearance
    of Borrelia burgdorferi from the blood circulation. <i>Parasites &#38; vectors</i>
    13, Nr. 1. doi:<a href="https://doi.org/10.1186/s13071-020-04060-y">10.1186/s13071-020-04060-y</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Liang, Liucun</span> ; <span
    style="font-variant:small-caps;">Wang, Jinyong</span> ; <span style="font-variant:small-caps;">Schorter,
    Lucas</span> ; <span style="font-variant:small-caps;">Nguyen Trong, Thu Phong</span>
    ; <span style="font-variant:small-caps;">Fell, Shari</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Straubinger, Reinhard
    K.</span>: Rapid clearance of Borrelia burgdorferi from the blood circulation.
    In: <i>Parasites &#38; vectors</i> Bd. 13. London, BioMed Central  (2020), Nr. 1'
  havard: L. Liang, J. Wang, L. Schorter, T.P. Nguyen Trong, S. Fell, S. Ulrich, R.K.
    Straubinger, Rapid clearance of Borrelia burgdorferi from the blood circulation,
    Parasites &#38; Vectors. 13 (2020).
  ieee: 'L. Liang <i>et al.</i>, “Rapid clearance of Borrelia burgdorferi from the
    blood circulation,” <i>Parasites &#38; vectors</i>, vol. 13, no. 1, Art. no. 191,
    2020, doi: <a href="https://doi.org/10.1186/s13071-020-04060-y">10.1186/s13071-020-04060-y</a>.'
  mla: Liang, Liucun, et al. “Rapid Clearance of Borrelia Burgdorferi from the Blood
    Circulation.” <i>Parasites &#38; Vectors</i>, vol. 13, no. 1, 191, 2020, <a href="https://doi.org/10.1186/s13071-020-04060-y">https://doi.org/10.1186/s13071-020-04060-y</a>.
  short: L. Liang, J. Wang, L. Schorter, T.P. Nguyen Trong, S. Fell, S. Ulrich, R.K.
    Straubinger, Parasites &#38; Vectors 13 (2020).
  ufg: '<b>Liang, Liucun u. a.</b>: Rapid clearance of Borrelia burgdorferi from the
    blood circulation, in: <i>Parasites &#38; vectors</i> 13 (2020), H. 1.'
  van: Liang L, Wang J, Schorter L, Nguyen Trong TP, Fell S, Ulrich S, et al. Rapid
    clearance of Borrelia burgdorferi from the blood circulation. Parasites &#38;
    vectors. 2020;13(1).
date_created: 2025-06-15T10:13:30Z
date_updated: 2025-06-17T14:17:27Z
department:
- _id: DEP4010
doi: 10.1186/s13071-020-04060-y
extern: '1'
intvolume: '        13'
issue: '1'
keyword:
- Lyme borreliosis
- Borrelia burgdorferi
- Tick-borne relapsing fever
- Borrelia persica
- Blood clearance
language:
- iso: eng
place: London
publication: Parasites & vectors
publication_identifier:
  issn:
  - 1756-3305
publication_status: published
publisher: 'BioMed Central '
quality_controlled: '1'
status: public
title: Rapid clearance of Borrelia burgdorferi from the blood circulation
type: scientific_journal_article
user_id: '83781'
volume: 13
year: '2020'
...
---
_id: '12963'
abstract:
- lang: eng
  text: The genus Borrelia comprises vector-borne bacterial pathogens that can severely
    affect human and animal health. Members of the Borrelia burgdorferi sensu lato
    species complex can cause Lyme borreliosis, one of the most common vector-borne
    diseases in the Northern hemisphere. Besides, members of the relapsing fever group
    of spirochetes can cause tick-borne relapsing fever in humans and various febrile
    illnesses in animals in tropical, subtropical and temperate regions. Borrelia
    spp. organisms are fastidious to cultivate and to maintain in vitro, and therefore,
    difficult to work with in the laboratory. Currently, borrelia identification is
    mainly performed using PCR and DNA sequencing methods, which can be complicated/frustrating
    on complex DNA templates and may still be relatively expensive. Alternative techniques
    such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    (MALDI-TOF MS) are not well established for Borrelia spp., although this technique
    is currently one of the most used techniques for rapid identification of bacteria
    in microbiological diagnostic laboratories. This is mainly due to unsatisfactory
    results obtained by use of simple sample preparation techniques and medium-contamination
    obscuring the mass spectra. In addition, comprehensive libraries for Borrelia
    spp. MALDI-TOF MS have yet to be established. In this study, we developed a new
    filter-based chemical extraction technique that allows measurement of high quality
    Borrelia spp. spectra from less than 100,000 bacteria per spot in MALDI-TOF MS.
    We used 49 isolates of 13 different species to produce the largest mass-library
    for Borrelia spp. so far and to validate the protocol. The library was successfully
    established and identifies >96% of used isolates correctly to species level. Cluster
    analysis on the sum spectra was applied to all the different isolates, which resulted
    in tight cluster generation for most species. Comparative analysis of the generated
    cluster to a phylogeny based on concatenated multi-locus sequence typing genes
    provided a surprising homology. Our data demonstrate that the technique described
    here can be used for fast and reliable species and strain typing within the borrelia
    complex.
author:
- first_name: Anna-Cathrine
  full_name: Neumann-Cip, Anna-Cathrine
  last_name: Neumann-Cip
- first_name: Volker
  full_name: Fingerle, Volker
  last_name: Fingerle
- first_name: Gabriele
  full_name: Margos, Gabriele
  last_name: Margos
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Evelyn
  full_name: Overzier, Evelyn
  last_name: Overzier
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Andreas
  full_name: Wieser, Andreas
  last_name: Wieser
citation:
  ama: Neumann-Cip AC, Fingerle V, Margos G, et al. A Novel Rapid Sample Preparation
    Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate
    Differentiation. <i>Frontiers in Microbiology</i>. 2020;11. doi:<a href="https://doi.org/10.3389/fmicb.2020.00690">10.3389/fmicb.2020.00690</a>
  apa: Neumann-Cip, A.-C., Fingerle, V., Margos, G., Straubinger, R. K., Overzier,
    E., Ulrich, S., &#38; Wieser, A. (2020). A Novel Rapid Sample Preparation Method
    for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation.
    <i>Frontiers in Microbiology</i>, <i>11</i>. <a href="https://doi.org/10.3389/fmicb.2020.00690">https://doi.org/10.3389/fmicb.2020.00690</a>
  bjps: <b>Neumann-Cip A-C <i>et al.</i></b> (2020) A Novel Rapid Sample Preparation
    Method for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate
    Differentiation. <i>Frontiers in Microbiology</i> <b>11</b>.
  chicago: Neumann-Cip, Anna-Cathrine, Volker Fingerle, Gabriele Margos, Reinhard
    K. Straubinger, Evelyn Overzier, Sebastian Ulrich, and Andreas Wieser. “A Novel
    Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia Burgdorferi
    Sensu Lato Species and Isolate Differentiation.” <i>Frontiers in Microbiology</i>
    11 (2020). <a href="https://doi.org/10.3389/fmicb.2020.00690">https://doi.org/10.3389/fmicb.2020.00690</a>.
  chicago-de: Neumann-Cip, Anna-Cathrine, Volker Fingerle, Gabriele Margos, Reinhard
    K. Straubinger, Evelyn Overzier, Sebastian Ulrich und Andreas Wieser. 2020. A
    Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi
    Sensu Lato Species and Isolate Differentiation. <i>Frontiers in Microbiology</i>
    11. doi:<a href="https://doi.org/10.3389/fmicb.2020.00690">10.3389/fmicb.2020.00690</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Neumann-Cip, Anna-Cathrine</span>
    ; <span style="font-variant:small-caps;">Fingerle, Volker</span> ; <span style="font-variant:small-caps;">Margos,
    Gabriele</span> ; <span style="font-variant:small-caps;">Straubinger, Reinhard
    K.</span> ; <span style="font-variant:small-caps;">Overzier, Evelyn</span> ; <span
    style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Wieser,
    Andreas</span>: A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits
    Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation. In: <i>Frontiers
    in Microbiology</i> Bd. 11. Lausanne, Frontiers Media SA (2020)'
  havard: A.-C. Neumann-Cip, V. Fingerle, G. Margos, R.K. Straubinger, E. Overzier,
    S. Ulrich, A. Wieser, A Novel Rapid Sample Preparation Method for MALDI-TOF MS
    Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation, Frontiers
    in Microbiology. 11 (2020).
  ieee: 'A.-C. Neumann-Cip <i>et al.</i>, “A Novel Rapid Sample Preparation Method
    for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation,”
    <i>Frontiers in Microbiology</i>, vol. 11, 2020, doi: <a href="https://doi.org/10.3389/fmicb.2020.00690">10.3389/fmicb.2020.00690</a>.'
  mla: Neumann-Cip, Anna-Cathrine, et al. “A Novel Rapid Sample Preparation Method
    for MALDI-TOF MS Permits Borrelia Burgdorferi Sensu Lato Species and Isolate Differentiation.”
    <i>Frontiers in Microbiology</i>, vol. 11, 2020, <a href="https://doi.org/10.3389/fmicb.2020.00690">https://doi.org/10.3389/fmicb.2020.00690</a>.
  short: A.-C. Neumann-Cip, V. Fingerle, G. Margos, R.K. Straubinger, E. Overzier,
    S. Ulrich, A. Wieser, Frontiers in Microbiology 11 (2020).
  ufg: '<b>Neumann-Cip, Anna-Cathrine u. a.</b>: A Novel Rapid Sample Preparation
    Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate
    Differentiation, in: <i>Frontiers in Microbiology</i> 11 (2020).'
  van: Neumann-Cip AC, Fingerle V, Margos G, Straubinger RK, Overzier E, Ulrich S,
    et al. A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia
    burgdorferi Sensu Lato Species and Isolate Differentiation. Frontiers in Microbiology.
    2020;11.
date_created: 2025-06-15T10:15:09Z
date_updated: 2025-06-18T11:09:40Z
department:
- _id: DEP4010
doi: 10.3389/fmicb.2020.00690
extern: '1'
intvolume: '        11'
keyword:
- Borrelia burgdorferi sensu lato
- MALDI-TOF MS
- typing
- sample preparation
- MALDI-TOF MS library
- strain typing
- automatic identification
language:
- iso: eng
place: Lausanne
publication: Frontiers in Microbiology
publication_identifier:
  eissn:
  - 1664-302X
publication_status: published
publisher: Frontiers Media SA
quality_controlled: '1'
status: public
title: A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi
  Sensu Lato Species and Isolate Differentiation
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2020'
...
---
_id: '12964'
abstract:
- lang: eng
  text: Antibodies represent an important element in the adaptive immune response
    and a major tool to eliminate microbial pathogens. For many bacterial and viral
    infections, efficient vaccines exist, but not for fungal pathogens. For a long
    time, antibodies have been assumed to be of minor importance for a successful
    clearance of fungal infections; however this perception has been challenged by
    a large number of studies over the last three decades. In this review, we focus
    on the potential therapeutic and prophylactic use of monoclonal antibodies. Since
    systemic mycoses normally occur in severely immunocompromised patients, a passive
    immunization using monoclonal antibodies is a promising approach to directly attack
    the fungal pathogen and/or to activate and strengthen the residual antifungal
    immune response in these patients.
article_number: '22'
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Frank
  full_name: Ebel, Frank
  last_name: Ebel
citation:
  ama: Ulrich S, Ebel F. Monoclonal Antibodies as Tools to Combat Fungal Infections.
    <i>Journal of Fungi</i>. 2020;6(1). doi:<a href="https://doi.org/10.3390/jof6010022">10.3390/jof6010022</a>
  apa: Ulrich, S., &#38; Ebel, F. (2020). Monoclonal Antibodies as Tools to Combat
    Fungal Infections. <i>Journal of Fungi</i>, <i>6</i>(1), Article 22. <a href="https://doi.org/10.3390/jof6010022">https://doi.org/10.3390/jof6010022</a>
  bjps: <b>Ulrich S and Ebel F</b> (2020) Monoclonal Antibodies as Tools to Combat
    Fungal Infections. <i>Journal of Fungi</i> <b>6</b>.
  chicago: Ulrich, Sebastian, and Frank Ebel. “Monoclonal Antibodies as Tools to Combat
    Fungal Infections.” <i>Journal of Fungi</i> 6, no. 1 (2020). <a href="https://doi.org/10.3390/jof6010022">https://doi.org/10.3390/jof6010022</a>.
  chicago-de: Ulrich, Sebastian und Frank Ebel. 2020. Monoclonal Antibodies as Tools
    to Combat Fungal Infections. <i>Journal of Fungi</i> 6, Nr. 1. doi:<a href="https://doi.org/10.3390/jof6010022">10.3390/jof6010022</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Ebel, Frank</span>: Monoclonal Antibodies
    as Tools to Combat Fungal Infections. In: <i>Journal of Fungi</i> Bd. 6. Basel,
    MDPI  (2020), Nr. 1'
  havard: S. Ulrich, F. Ebel, Monoclonal Antibodies as Tools to Combat Fungal Infections,
    Journal of Fungi. 6 (2020).
  ieee: 'S. Ulrich and F. Ebel, “Monoclonal Antibodies as Tools to Combat Fungal Infections,”
    <i>Journal of Fungi</i>, vol. 6, no. 1, Art. no. 22, 2020, doi: <a href="https://doi.org/10.3390/jof6010022">10.3390/jof6010022</a>.'
  mla: Ulrich, Sebastian, and Frank Ebel. “Monoclonal Antibodies as Tools to Combat
    Fungal Infections.” <i>Journal of Fungi</i>, vol. 6, no. 1, 22, 2020, <a href="https://doi.org/10.3390/jof6010022">https://doi.org/10.3390/jof6010022</a>.
  short: S. Ulrich, F. Ebel, Journal of Fungi 6 (2020).
  ufg: '<b>Ulrich, Sebastian/Ebel, Frank</b>: Monoclonal Antibodies as Tools to Combat
    Fungal Infections, in: <i>Journal of Fungi</i> 6 (2020), H. 1.'
  van: Ulrich S, Ebel F. Monoclonal Antibodies as Tools to Combat Fungal Infections.
    Journal of Fungi. 2020;6(1).
date_created: 2025-06-15T10:17:00Z
date_updated: 2025-06-18T11:13:40Z
department:
- _id: DEP4010
doi: 10.3390/jof6010022
extern: '1'
intvolume: '         6'
issue: '1'
keyword:
- monoclonal antibodies
- invasive fungal infections
- therapy
- prophylaxis
- opsonization
language:
- iso: eng
place: Basel
publication: Journal of Fungi
publication_identifier:
  eissn:
  - 2309-608X
publication_status: published
publisher: 'MDPI '
quality_controlled: '1'
status: public
title: Monoclonal Antibodies as Tools to Combat Fungal Infections
type: scientific_journal_article
user_id: '83781'
volume: 6
year: '2020'
...
---
_id: '12965'
abstract:
- lang: eng
  text: "The Bacillus (B.) cereus group consists of nine recognized species which
    are present worldwide. B. cereus play an important role in food-borne diseases
    by producing different toxins. Yet, only a small percentage of B. cereus strains
    are able to produce the heat stable cereulide, the causative agent of emetic food
    poisoning. To minimize the entry of emetic B. cereus into the food chain, food
    business operators are dependent on efficient and reliable methods enabling the
    differentiation between emetic and non-emetic strains.\r\nCurrently, only time-consuming
    cell bioassays, molecular methods and tandem mass spectrometry are available for
    this purpose. Thus, the aim of the present study was to establish a fast and reliable
    method for the differentiation between emetic/non-emetic strains by MALDI-TOF
    MS. Selected strains/isolates of the B. cereus group as well as other Bacillus
    spp. (total n = 121) were cultured on sheep blood agar for 48 h before analysis.\r\nSubsequently,
    the cultures were directly analyzed by MALDI-TOF MS without prior extraction steps.
    The samples were measured in the mass range of m/z 800–1800 Da. Using ClinProTools
    3.0 statistical software and Flex analysis software (Bruker Daltonics GmbH, Bremen,
    Germany), a differentiation between emetic/non-emetic isolates was possible with
    a rate of correct identification of 99.1% by means of the evaluation of two specific
    biomarkers (m/z 1171 and 1187 Da)."
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Richard
  full_name: Dietrich, Richard
  last_name: Dietrich
- first_name: Erwin
  full_name: Märtlbauer, Erwin
  last_name: Märtlbauer
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
citation:
  ama: Ulrich S, Gottschalk C, Dietrich R, Märtlbauer E, Gareis M. Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS. <i>Food Microbiology</i>.
    2019;82:75-81. doi:<a href="https://doi.org/10.1016/j.fm.2019.01.012">10.1016/j.fm.2019.01.012</a>
  apa: Ulrich, S., Gottschalk, C., Dietrich, R., Märtlbauer, E., &#38; Gareis, M.
    (2019). Identification of cereulide producing Bacillus cereus by MALDI-TOF MS.
    <i>Food Microbiology</i>, <i>82</i>, 75–81. <a href="https://doi.org/10.1016/j.fm.2019.01.012">https://doi.org/10.1016/j.fm.2019.01.012</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2019) Identification of Cereulide Producing
    Bacillus Cereus by MALDI-TOF MS. <i>Food Microbiology</i> <b>82</b>, 75–81.
  chicago: 'Ulrich, Sebastian, Christoph Gottschalk, Richard Dietrich, Erwin Märtlbauer,
    and Manfred Gareis. “Identification of Cereulide Producing Bacillus Cereus by
    MALDI-TOF MS.” <i>Food Microbiology</i> 82 (2019): 75–81. <a href="https://doi.org/10.1016/j.fm.2019.01.012">https://doi.org/10.1016/j.fm.2019.01.012</a>.'
  chicago-de: 'Ulrich, Sebastian, Christoph Gottschalk, Richard Dietrich, Erwin Märtlbauer
    und Manfred Gareis. 2019. Identification of cereulide producing Bacillus cereus
    by MALDI-TOF MS. <i>Food Microbiology</i> 82: 75–81. doi:<a href="https://doi.org/10.1016/j.fm.2019.01.012">10.1016/j.fm.2019.01.012</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Gottschalk, Christoph</span> ; <span style="font-variant:small-caps;">Dietrich,
    Richard</span> ; <span style="font-variant:small-caps;">Märtlbauer, Erwin</span>
    ; <span style="font-variant:small-caps;">Gareis, Manfred</span>: Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS. In: <i>Food Microbiology</i>
    Bd. 82. London, Academic Press  (2019), S. 75–81'
  havard: S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, M. Gareis, Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS, Food Microbiology. 82
    (2019) 75–81.
  ieee: 'S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, and M. Gareis, “Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS,” <i>Food Microbiology</i>,
    vol. 82, pp. 75–81, 2019, doi: <a href="https://doi.org/10.1016/j.fm.2019.01.012">10.1016/j.fm.2019.01.012</a>.'
  mla: Ulrich, Sebastian, et al. “Identification of Cereulide Producing Bacillus Cereus
    by MALDI-TOF MS.” <i>Food Microbiology</i>, vol. 82, 2019, pp. 75–81, <a href="https://doi.org/10.1016/j.fm.2019.01.012">https://doi.org/10.1016/j.fm.2019.01.012</a>.
  short: S. Ulrich, C. Gottschalk, R. Dietrich, E. Märtlbauer, M. Gareis, Food Microbiology
    82 (2019) 75–81.
  ufg: '<b>Ulrich, Sebastian u. a.</b>: Identification of cereulide producing Bacillus
    cereus by MALDI-TOF MS, in: <i>Food Microbiology</i> 82 (2019),  S. 75–81.'
  van: Ulrich S, Gottschalk C, Dietrich R, Märtlbauer E, Gareis M. Identification
    of cereulide producing Bacillus cereus by MALDI-TOF MS. Food Microbiology. 2019;82:75–81.
date_created: 2025-06-15T10:18:20Z
date_updated: 2025-06-18T11:16:35Z
department:
- _id: DEP4010
doi: 10.1016/j.fm.2019.01.012
extern: '1'
intvolume: '        82'
keyword:
- MALDI-TOF MS
- Bacillus cereus
- Cereulide
- Food intoxication
language:
- iso: eng
page: 75-81
place: London
publication: Food Microbiology
publication_identifier:
  eissn:
  - 1095-9998
  issn:
  - 0740-0020
publication_status: published
publisher: 'Academic Press '
quality_controlled: '1'
status: public
title: Identification of cereulide producing Bacillus cereus by MALDI-TOF MS
type: scientific_journal_article
user_id: '83781'
volume: 82
year: '2019'
...
---
_id: '12966'
abstract:
- lang: eng
  text: 'The fungus Stachybotrys (S.) chartarum was isolated from culinary herbs,
    damp building materials, and improperly stored animal forage. Two distinct chemotypes
    of the fungus were described that produced either high-cytotoxic macrocyclic trichothecenes
    (S type) or low-cytotoxic atranones (A type). Recently, two distinct gene clusters
    were described that were found to be necessary for the biosynthesis of either
    macrocyclic trichothecenes (21 SAT (Satratoxin) genes) or atranones (14 ATR (Atranone)
    genes). In the current study, PCR primers were designed to detect SAT and ATR
    genes in 19 S. chartarum chemotype S and eight S. chartarum chemotype A strains.
    Our analysis revealed the existence of three different genotypes: satratoxin-producing
    strains that harbored all SAT genes but lacked the ATR gene cluster (genotype
    S), non-satratoxin-producing strains that possessed the ATR genes but lacked SAT
    genes (genotype A), and a hitherto undescribed hybrid genotype among non-satratoxin-producing
    strains that harbored all ATR genes and an incomplete set of SAT genes (genotype
    H). In order to improve the discrimination of genotypes, a triplex PCR assay was
    developed and applied for the analysis of S. chartarum and S. chlorohalonata cultures.
    The results show that genes for macrocyclic trichothecenes and atranones are not
    mutually exclusive in S. chartarum. Correlation of the new genotype-based concept
    with mycotoxin production data shows also that macrocyclic trichothecenes are
    exclusively produced by S. chartarum genotype S strains.'
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Ludwig
  full_name: Niessen, Ludwig
  last_name: Niessen
- first_name: Julia
  full_name: Ekruth, Julia
  last_name: Ekruth
- first_name: Cornelius
  full_name: Schäfer, Cornelius
  last_name: Schäfer
- first_name: Florian
  full_name: Kaltner, Florian
  last_name: Kaltner
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
citation:
  ama: Ulrich S, Niessen L, Ekruth J, Schäfer C, Kaltner F, Gottschalk C. Truncated
    satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys
    chartarum (Ehrenb.) S. Hughes. <i>Mycotoxin Research</i>. 2019;36(1):83-91. doi:<a
    href="https://doi.org/10.1007/s12550-019-00371-x">10.1007/s12550-019-00371-x</a>
  apa: Ulrich, S., Niessen, L., Ekruth, J., Schäfer, C., Kaltner, F., &#38; Gottschalk,
    C. (2019). Truncated satratoxin gene clusters in selected isolates of the atranone
    chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. <i>Mycotoxin Research</i>,
    <i>36</i>(1), 83–91. <a href="https://doi.org/10.1007/s12550-019-00371-x">https://doi.org/10.1007/s12550-019-00371-x</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2019) Truncated Satratoxin Gene Clusters in
    Selected Isolates of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.)
    S. Hughes. <i>Mycotoxin Research</i> <b>36</b>, 83–91.
  chicago: 'Ulrich, Sebastian, Ludwig Niessen, Julia Ekruth, Cornelius Schäfer, Florian
    Kaltner, and Christoph Gottschalk. “Truncated Satratoxin Gene Clusters in Selected
    Isolates of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.) S. Hughes.”
    <i>Mycotoxin Research</i> 36, no. 1 (2019): 83–91. <a href="https://doi.org/10.1007/s12550-019-00371-x">https://doi.org/10.1007/s12550-019-00371-x</a>.'
  chicago-de: 'Ulrich, Sebastian, Ludwig Niessen, Julia Ekruth, Cornelius Schäfer,
    Florian Kaltner und Christoph Gottschalk. 2019. Truncated satratoxin gene clusters
    in selected isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.)
    S. Hughes. <i>Mycotoxin Research</i> 36, Nr. 1: 83–91. doi:<a href="https://doi.org/10.1007/s12550-019-00371-x">10.1007/s12550-019-00371-x</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Niessen, Ludwig</span> ; <span style="font-variant:small-caps;">Ekruth,
    Julia</span> ; <span style="font-variant:small-caps;">Schäfer, Cornelius</span>
    ; <span style="font-variant:small-caps;">Kaltner, Florian</span> ; <span style="font-variant:small-caps;">Gottschalk,
    Christoph</span>: Truncated satratoxin gene clusters in selected isolates of the
    atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes. In: <i>Mycotoxin
    Research</i> Bd. 36. Berlin ; Heidelberg, Springer (2019), Nr. 1, S. 83–91'
  havard: S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, C. Gottschalk,
    Truncated satratoxin gene clusters in selected isolates of the atranone chemotype
    of Stachybotrys chartarum (Ehrenb.) S. Hughes, Mycotoxin Research. 36 (2019) 83–91.
  ieee: 'S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, and C. Gottschalk,
    “Truncated satratoxin gene clusters in selected isolates of the atranone chemotype
    of Stachybotrys chartarum (Ehrenb.) S. Hughes,” <i>Mycotoxin Research</i>, vol.
    36, no. 1, pp. 83–91, 2019, doi: <a href="https://doi.org/10.1007/s12550-019-00371-x">10.1007/s12550-019-00371-x</a>.'
  mla: Ulrich, Sebastian, et al. “Truncated Satratoxin Gene Clusters in Selected Isolates
    of the Atranone Chemotype of Stachybotrys Chartarum (Ehrenb.) S. Hughes.” <i>Mycotoxin
    Research</i>, vol. 36, no. 1, 2019, pp. 83–91, <a href="https://doi.org/10.1007/s12550-019-00371-x">https://doi.org/10.1007/s12550-019-00371-x</a>.
  short: S. Ulrich, L. Niessen, J. Ekruth, C. Schäfer, F. Kaltner, C. Gottschalk,
    Mycotoxin Research 36 (2019) 83–91.
  ufg: '<b>Ulrich, Sebastian u. a.</b>: Truncated satratoxin gene clusters in selected
    isolates of the atranone chemotype of Stachybotrys chartarum (Ehrenb.) S. Hughes,
    in: <i>Mycotoxin Research</i> 36 (2019), H. 1,  S. 83–91.'
  van: Ulrich S, Niessen L, Ekruth J, Schäfer C, Kaltner F, Gottschalk C. Truncated
    satratoxin gene clusters in selected isolates of the atranone chemotype of Stachybotrys
    chartarum (Ehrenb.) S. Hughes. Mycotoxin Research. 2019;36(1):83–91.
date_created: 2025-06-15T10:19:53Z
date_updated: 2025-06-18T11:20:11Z
department:
- _id: DEP4010
doi: 10.1007/s12550-019-00371-x
extern: '1'
intvolume: '        36'
issue: '1'
keyword:
- Acetyltransferases
- Chlamydomonas reinhardtii
- Fungal Genes
- Fungal genetics
- Fungal genomics
- Saccharomyces cerevisiae
language:
- iso: eng
page: 83-91
place: Berlin ; Heidelberg
publication: Mycotoxin Research
publication_identifier:
  eissn:
  - 1867-1632
  issn:
  - 0178-7888
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: Truncated satratoxin gene clusters in selected isolates of the atranone chemotype
  of Stachybotrys chartarum (Ehrenb.) S. Hughes
type: scientific_journal_article
user_id: '83781'
volume: 36
year: '2019'
...
---
_id: '12967'
abstract:
- lang: eng
  text: "Objectives\r\nTo evaluate matrix-assisted laser desorption ionisation time
    of flight mass spectrometry (MALDI-TOF MS) combined with the Sepsityper kit (Bruker
    Daltoniks GmbH, Bremen) for the direct detection of bacterial species from inoculated
    blood cultures from dogs and cats.\r\nMaterials and Methods\r\nCanine and feline
    blood samples were inoculated with typical sepsis-causing bacteria such as Staphylococcus
    intermedius, Staphylococcus aureus, Streptococcus canis, Enterococcus faecalis,
    Escherichia coli and Pseudomonas aeruginosa at two distinct concentrations (each
    in triplicate), resulting in 72 blood culture bottles incubated at 37°C. Samples
    were comparatively analysed with MALDI-TOF MS after preparation with the Sepsityper
    kit and also by standard bacteriology (culturing and biochemical characterisation).\r\nResults\r\nBacterial
    species identified from agar plates and by MALDI-TOF MS from blood culture bottles
    were identical for all samples. The MALDI Biotyper software (Bruker Daltoniks)
    correctly identified all bacterial strains from inoculated canine and feline blood
    with analysis indicating very good precision.\r\nClinical Significance\r\nMALDI-TOF
    MS analysis combined with the Sepsityper kit is a reliable tool for a quick detection
    of veterinary-relevant bacterial species directly from blood culture bottles.
    This approach could reduce the time for identification of critical species to
    only 24 hours."
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: C.
  full_name: Gottschalk, C.
  last_name: Gottschalk
- first_name: R. Kk
  full_name: Straubinger, R. Kk
  last_name: Straubinger
- first_name: K.
  full_name: Schwaiger, K.
  last_name: Schwaiger
- first_name: R.
  full_name: Dörfelt, R.
  last_name: Dörfelt
citation:
  ama: Ulrich S, Gottschalk C, Straubinger RK, Schwaiger K, Dörfelt R. Acceleration
    of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood.
    <i>Journal of Small Animal Practice</i>. 2019;61(1):42-45. doi:<a href="https://doi.org/10.1111/jsap.13056">10.1111/jsap.13056</a>
  apa: Ulrich, S., Gottschalk, C., Straubinger, R. K., Schwaiger, K., &#38; Dörfelt,
    R. (2019). Acceleration of the identification of sepsis‐inducing bacteria in cultures
    of dog and cat blood. <i>Journal of Small Animal Practice</i>, <i>61</i>(1), 42–45.
    <a href="https://doi.org/10.1111/jsap.13056">https://doi.org/10.1111/jsap.13056</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2019) Acceleration of the Identification of
    Sepsis‐inducing Bacteria in Cultures of Dog and Cat Blood. <i>Journal of Small
    Animal Practice</i> <b>61</b>, 42–45.
  chicago: 'Ulrich, Sebastian, C. Gottschalk, R. Kk Straubinger, K. Schwaiger, and
    R. Dörfelt. “Acceleration of the Identification of Sepsis‐inducing Bacteria in
    Cultures of Dog and Cat Blood.” <i>Journal of Small Animal Practice</i> 61, no.
    1 (2019): 42–45. <a href="https://doi.org/10.1111/jsap.13056">https://doi.org/10.1111/jsap.13056</a>.'
  chicago-de: 'Ulrich, Sebastian, C. Gottschalk, R. Kk Straubinger, K. Schwaiger und
    R. Dörfelt. 2019. Acceleration of the identification of sepsis‐inducing bacteria
    in cultures of dog and cat blood. <i>Journal of Small Animal Practice</i> 61,
    Nr. 1: 42–45. doi:<a href="https://doi.org/10.1111/jsap.13056">10.1111/jsap.13056</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Gottschalk, C.</span> ; <span style="font-variant:small-caps;">Straubinger,
    R. Kk</span> ; <span style="font-variant:small-caps;">Schwaiger, K.</span> ; <span
    style="font-variant:small-caps;">Dörfelt, R.</span>: Acceleration of the identification
    of sepsis‐inducing bacteria in cultures of dog and cat blood. In: <i>Journal of
    Small Animal Practice</i> Bd. 61. Oxford [u.a.], Wiley-Blackwell (2019), Nr. 1,
    S. 42–45'
  havard: S. Ulrich, C. Gottschalk, R.K. Straubinger, K. Schwaiger, R. Dörfelt, Acceleration
    of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood,
    Journal of Small Animal Practice. 61 (2019) 42–45.
  ieee: 'S. Ulrich, C. Gottschalk, R. K. Straubinger, K. Schwaiger, and R. Dörfelt,
    “Acceleration of the identification of sepsis‐inducing bacteria in cultures of dog
    and cat blood,” <i>Journal of Small Animal Practice</i>, vol. 61, no. 1, pp. 42–45,
    2019, doi: <a href="https://doi.org/10.1111/jsap.13056">10.1111/jsap.13056</a>.'
  mla: Ulrich, Sebastian, et al. “Acceleration of the Identification of Sepsis‐inducing
    Bacteria in Cultures of Dog and Cat Blood.” <i>Journal of Small Animal Practice</i>,
    vol. 61, no. 1, 2019, pp. 42–45, <a href="https://doi.org/10.1111/jsap.13056">https://doi.org/10.1111/jsap.13056</a>.
  short: S. Ulrich, C. Gottschalk, R.K. Straubinger, K. Schwaiger, R. Dörfelt, Journal
    of Small Animal Practice 61 (2019) 42–45.
  ufg: '<b>Ulrich, Sebastian u. a.</b>: Acceleration of the identification of sepsis‐inducing
    bacteria in cultures of dog and cat blood, in: <i>Journal of Small Animal Practice</i>
    61 (2019), H. 1,  S. 42–45.'
  van: Ulrich S, Gottschalk C, Straubinger RK, Schwaiger K, Dörfelt R. Acceleration
    of the identification of sepsis‐inducing bacteria in cultures of dog and cat blood.
    Journal of Small Animal Practice. 2019;61(1):42–5.
date_created: 2025-06-15T10:20:55Z
date_updated: 2025-06-18T11:24:12Z
department:
- _id: DEP4010
doi: 10.1111/jsap.13056
extern: '1'
intvolume: '        61'
issue: '1'
language:
- iso: eng
page: 42-45
place: Oxford [u.a.]
publication: Journal of Small Animal Practice
publication_identifier:
  issn:
  - 0022-4510
  - 1748-5827
publication_status: published
publisher: Wiley-Blackwell
quality_controlled: '1'
status: public
title: Acceleration of the identification of sepsis‐inducing bacteria in cultures
  of dog and cat blood
type: scientific_journal_article
user_id: '83781'
volume: 61
year: '2019'
...
---
_id: '12971'
abstract:
- lang: eng
  text: The genus Stachybotrys produces a broad diversity of secondary metabolites,
    including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although
    the class of the phenylspirodrimanes is the major one and consists of a multitude
    of metabolites bearing various structural modifications, few investigations have
    been carried out. Thus, the presented study deals with the quantitative determination
    of several secondary metabolites produced by distinct Stachybotrys species for
    comparison of their metabolite profiles. For that purpose, 15 of the primarily
    produced secondary metabolites were isolated from fungal cultures and structurally
    characterized in order to be used as analytical standards for the development
    of an LC-MS/MS multimethod. The developed method was applied to the analysis of
    micro-scale extracts from 5 different Stachybotrys strains, which were cultured
    on different media. In that process, spontaneous dialdehyde/lactone isomerization
    was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes
    were quantitatively investigated for the first time. The metabolite profiles of
    Stachybotrys species are considerably influenced by time of growth and substrate
    availability, as well as the individual biosynthetic potential of the respective
    species. Regarding the reported adverse effects associated with Stachybotrys growth
    in building environments, combinatory effects of the investigated secondary metabolites
    should be addressed and the role of the phenylspirodrimanes re-evaluated in future
    research.
article_number: '133'
author:
- first_name: Annika
  full_name: Jagels, Annika
  last_name: Jagels
- first_name: Viktoria
  full_name: Lindemann, Viktoria
  last_name: Lindemann
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Benedikt
  full_name: Cramer, Benedikt
  last_name: Cramer
- first_name: Florian
  full_name: Hübner, Florian
  last_name: Hübner
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
- first_name: Hans-Ulrich
  full_name: Humpf, Hans-Ulrich
  last_name: Humpf
citation:
  ama: Jagels A, Lindemann V, Ulrich S, et al. Exploring Secondary Metabolite Profiles
    of Stachybotrys spp. by LC-MS/MS. <i>Toxins</i>. 2019;11(3). doi:<a href="https://doi.org/10.3390/toxins11030133">10.3390/toxins11030133</a>
  apa: Jagels, A., Lindemann, V., Ulrich, S., Gottschalk, C., Cramer, B., Hübner,
    F., Gareis, M., &#38; Humpf, H.-U. (2019). Exploring Secondary Metabolite Profiles
    of Stachybotrys spp. by LC-MS/MS. <i>Toxins</i>, <i>11</i>(3), Article 133. <a
    href="https://doi.org/10.3390/toxins11030133">https://doi.org/10.3390/toxins11030133</a>
  bjps: <b>Jagels A <i>et al.</i></b> (2019) Exploring Secondary Metabolite Profiles
    of Stachybotrys Spp. by LC-MS/MS. <i>Toxins</i> <b>11</b>.
  chicago: Jagels, Annika, Viktoria Lindemann, Sebastian Ulrich, Christoph Gottschalk,
    Benedikt Cramer, Florian Hübner, Manfred Gareis, and Hans-Ulrich Humpf. “Exploring
    Secondary Metabolite Profiles of Stachybotrys Spp. by LC-MS/MS.” <i>Toxins</i>
    11, no. 3 (2019). <a href="https://doi.org/10.3390/toxins11030133">https://doi.org/10.3390/toxins11030133</a>.
  chicago-de: Jagels, Annika, Viktoria Lindemann, Sebastian Ulrich, Christoph Gottschalk,
    Benedikt Cramer, Florian Hübner, Manfred Gareis und Hans-Ulrich Humpf. 2019. Exploring
    Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. <i>Toxins</i>
    11, Nr. 3. doi:<a href="https://doi.org/10.3390/toxins11030133">10.3390/toxins11030133</a>,
    .
  din1505-2-1: '<span style="font-variant:small-caps;">Jagels, Annika</span> ; <span
    style="font-variant:small-caps;">Lindemann, Viktoria</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Gottschalk, Christoph</span>
    ; <span style="font-variant:small-caps;">Cramer, Benedikt</span> ; <span style="font-variant:small-caps;">Hübner,
    Florian</span> ; <span style="font-variant:small-caps;">Gareis, Manfred</span>
    ; <span style="font-variant:small-caps;">Humpf, Hans-Ulrich</span>: Exploring
    Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. In: <i>Toxins</i>
    Bd. 11. Basel, MDPI (2019), Nr. 3'
  havard: A. Jagels, V. Lindemann, S. Ulrich, C. Gottschalk, B. Cramer, F. Hübner,
    M. Gareis, H.-U. Humpf, Exploring Secondary Metabolite Profiles of Stachybotrys
    spp. by LC-MS/MS, Toxins. 11 (2019).
  ieee: 'A. Jagels <i>et al.</i>, “Exploring Secondary Metabolite Profiles of Stachybotrys
    spp. by LC-MS/MS,” <i>Toxins</i>, vol. 11, no. 3, Art. no. 133, 2019, doi: <a
    href="https://doi.org/10.3390/toxins11030133">10.3390/toxins11030133</a>.'
  mla: Jagels, Annika, et al. “Exploring Secondary Metabolite Profiles of Stachybotrys
    Spp. by LC-MS/MS.” <i>Toxins</i>, vol. 11, no. 3, 133, 2019, <a href="https://doi.org/10.3390/toxins11030133">https://doi.org/10.3390/toxins11030133</a>.
  short: A. Jagels, V. Lindemann, S. Ulrich, C. Gottschalk, B. Cramer, F. Hübner,
    M. Gareis, H.-U. Humpf, Toxins 11 (2019).
  ufg: '<b>Jagels, Annika u. a.</b>: Exploring Secondary Metabolite Profiles of Stachybotrys
    spp. by LC-MS/MS, in: <i>Toxins</i> 11 (2019), H. 3.'
  van: Jagels A, Lindemann V, Ulrich S, Gottschalk C, Cramer B, Hübner F, et al. Exploring
    Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS. Toxins. 2019;11(3).
date_created: 2025-06-15T10:23:36Z
date_updated: 2025-06-17T14:12:55Z
department:
- _id: DEP4010
doi: 10.3390/toxins11030133
extern: '1'
intvolume: '        11'
issue: '3'
keyword:
- Stachybotrys spp.
- metabolite profiles
- LC-MS/MS
- satratoxins
- phenylspirodrimanes
- stachybotrychromenes
- biosynthetic production
language:
- iso: eng
place: Basel
publication: Toxins
publication_identifier:
  eissn:
  - 2072-6651
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS
type: scientific_journal_article
user_id: '83781'
volume: 11
year: '2019'
...
---
_id: '12973'
abstract:
- lang: eng
  text: 'Psychrophilic and psychrotolerant clostridia (n = 110) were isolated from
    vacuum-packed meat (beef and lamb), fresh venison and from skin and fecal samples
    of wild boars. They were identified to species level using MALDI-TOF MS, sequence
    and phylogeny analysis of the 16S rRNA and species specific multiplex qPCR. The
    results of all three methods were concordant. The majority of isolates were identified
    as C. tagluense-like Group I (n = 34) and Group II (n = 42). Thirty-five isolates
    could be identified to species level as follows: C. estertheticum (n = 15), C.
    frigoriphilum (n = 13), C. frigidicarnis (n = 1) and C. bowmanii (n = 5). This
    is the first report of detection and identification of C. frigoriphilum and C.
    tagluense-like Group II as causative agents of blown pack spoilage of beef. The
    species specific multiplex qPCR developed in this study could be applied to identify
    and to quantify the Clostridium species described above in suspicious meat juice
    samples.'
author:
- first_name: Samart
  full_name: Dorn-In, Samart
  last_name: Dorn-In
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Claudia
  full_name: Springer, Claudia
  last_name: Springer
- first_name: Leonard
  full_name: Barta, Leonard
  last_name: Barta
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
citation:
  ama: Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development
    of a multiplex qPCR for the species identification of Clostridium estertheticum,
    C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
    meats and from wild boars. <i>International Journal of Food Microbiology</i>.
    2018;286:162-169. doi:<a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">10.1016/j.ijfoodmicro.2018.08.020</a>
  apa: Dorn-In, S., Schwaiger, K., Springer, C., Barta, L., Ulrich, S., &#38; Gareis,
    M. (2018). Development of a multiplex qPCR for the species identification of Clostridium
    estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown
    pack spoilage (BPS) meats and from wild boars. <i>International Journal of Food
    Microbiology</i>, <i>286</i>, 162–169. <a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">https://doi.org/10.1016/j.ijfoodmicro.2018.08.020</a>
  bjps: <b>Dorn-In S <i>et al.</i></b> (2018) Development of a Multiplex QPCR for
    the Species Identification of Clostridium Estertheticum, C. Frigoriphilum, C.
    Bowmanii and C. Tagluense-like from Blown Pack Spoilage (BPS) Meats and from Wild
    Boars. <i>International Journal of Food Microbiology</i> <b>286</b>, 162–169.
  chicago: 'Dorn-In, Samart, Karin Schwaiger, Claudia Springer, Leonard Barta, Sebastian
    Ulrich, and Manfred Gareis. “Development of a Multiplex QPCR for the Species Identification
    of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like
    from Blown Pack Spoilage (BPS) Meats and from Wild Boars.” <i>International Journal
    of Food Microbiology</i> 286 (2018): 162–69. <a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">https://doi.org/10.1016/j.ijfoodmicro.2018.08.020</a>.'
  chicago-de: 'Dorn-In, Samart, Karin Schwaiger, Claudia Springer, Leonard Barta,
    Sebastian Ulrich und Manfred Gareis. 2018. Development of a multiplex qPCR for
    the species identification of Clostridium estertheticum, C. frigoriphilum, C.
    bowmanii and C. tagluense-like from blown pack spoilage (BPS) meats and from wild
    boars. <i>International Journal of Food Microbiology</i> 286: 162–169. doi:<a
    href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">10.1016/j.ijfoodmicro.2018.08.020</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Dorn-In, Samart</span> ; <span
    style="font-variant:small-caps;">Schwaiger, Karin</span> ; <span style="font-variant:small-caps;">Springer,
    Claudia</span> ; <span style="font-variant:small-caps;">Barta, Leonard</span>
    ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span> ; <span style="font-variant:small-caps;">Gareis,
    Manfred</span>: Development of a multiplex qPCR for the species identification
    of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like
    from blown pack spoilage (BPS) meats and from wild boars. In: <i>International
    Journal of Food Microbiology</i> Bd. 286. Amsterdam, Elsevier  (2018), S. 162–169'
  havard: S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, M. Gareis, Development
    of a multiplex qPCR for the species identification of Clostridium estertheticum,
    C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
    meats and from wild boars, International Journal of Food Microbiology. 286 (2018)
    162–169.
  ieee: 'S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, and M. Gareis,
    “Development of a multiplex qPCR for the species identification of Clostridium
    estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown
    pack spoilage (BPS) meats and from wild boars,” <i>International Journal of Food
    Microbiology</i>, vol. 286, pp. 162–169, 2018, doi: <a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">10.1016/j.ijfoodmicro.2018.08.020</a>.'
  mla: Dorn-In, Samart, et al. “Development of a Multiplex QPCR for the Species Identification
    of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like
    from Blown Pack Spoilage (BPS) Meats and from Wild Boars.” <i>International Journal
    of Food Microbiology</i>, vol. 286, 2018, pp. 162–69, <a href="https://doi.org/10.1016/j.ijfoodmicro.2018.08.020">https://doi.org/10.1016/j.ijfoodmicro.2018.08.020</a>.
  short: S. Dorn-In, K. Schwaiger, C. Springer, L. Barta, S. Ulrich, M. Gareis, International
    Journal of Food Microbiology 286 (2018) 162–169.
  ufg: '<b>Dorn-In, Samart u. a.</b>: Development of a multiplex qPCR for the species
    identification of Clostridium estertheticum, C. frigoriphilum, C. bowmanii and
    C. tagluense-like from blown pack spoilage (BPS) meats and from wild boars, in:
    <i>International Journal of Food Microbiology</i> 286 (2018),  S. 162–169.'
  van: Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development
    of a multiplex qPCR for the species identification of Clostridium estertheticum,
    C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack spoilage (BPS)
    meats and from wild boars. International Journal of Food Microbiology. 2018;286:162–9.
date_created: 2025-06-15T10:25:24Z
date_updated: 2025-06-18T11:27:12Z
department:
- _id: DEP4010
doi: 10.1016/j.ijfoodmicro.2018.08.020
extern: '1'
intvolume: '       286'
keyword:
- Blown pack spoilage
- MALDI-TOF MS
- PCR
- 16S rRNA
- C. frigoriphilum
- C. tagluense-like
language:
- iso: eng
page: 162-169
place: Amsterdam
publication: International Journal of Food Microbiology
publication_identifier:
  eissn:
  - 1879-3460
  issn:
  - 0168-1605
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Development of a multiplex qPCR for the species identification of Clostridium
  estertheticum, C. frigoriphilum, C. bowmanii and C. tagluense-like from blown pack
  spoilage (BPS) meats and from wild boars
type: scientific_journal_article
user_id: '83781'
volume: 286
year: '2018'
...
---
_id: '12974'
abstract:
- lang: eng
  text: "BACKGROUND\r\nFruits and vegetables have increasingly been related to foodborne
    outbreaks. Besides surface contamination, a possible internalization of microorganisms
    into edible parts of plants during growth has already been observed. To examine
    an actual risk for the consumer, microbial contamination of the rind and pulp
    of 147 muskmelons from international trade was assessed using cultural and biochemical
    methods, polymerase chain reaction and matrix-assisted laser desorption/ionization-time
    of flight mass spectrometry.\r\nRESULTS\r\nOne hundred percent of the rind samples
    [3.69–8.92 log colony forming units (CFU) g−1] and 89.8% of the pulp samples (maximum
    load 3.66 log CFU g−1) were microbiologically contaminated. Among the 432 pulp
    isolates, opportunistic and potentially pathogenic bacteria were identified, mainly
    Staphylococcus spp. (48.9%), Clostridium spp. (42.9%) and Enterobacteriaceae (27.9%).
    Salmonella spp., Escherichia coli and isolates of the Bacillus cereus group were
    found on the rind (1.4%, 0.7% and 42.9%, respectively) and in the pulp (0.7%,
    1.4% and 4.7%). Clostridium perfringens was isolated from the rind of seven melons.\r\nCONCLUSION\r\nThe
    present study revealed a regularly occurring internal contamination of melons.
    Possible health risks for consumers because of an occurrence of microorganisms
    in melon pulp should be considered in future food safety assessments. © 2018 Society
    of Chemical Industry."
author:
- first_name: Irene
  full_name: Esteban‐Cuesta, Irene
  last_name: Esteban‐Cuesta
- first_name: Nathalie
  full_name: Drees, Nathalie
  last_name: Drees
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Peter
  full_name: Stauch, Peter
  last_name: Stauch
- first_name: Brigitte
  full_name: Sperner, Brigitte
  last_name: Sperner
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
citation:
  ama: 'Esteban‐Cuesta I, Drees N, Ulrich S, et al. Endogenous microbial contamination
    of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk
    for the consumer? <i>Journal of the science of food and agriculture : incorporating
    Agri-Biotech</i>. 2018;98(13):5074-5081. doi:<a href="https://doi.org/10.1002/jsfa.9045">10.1002/jsfa.9045</a>'
  apa: 'Esteban‐Cuesta, I., Drees, N., Ulrich, S., Stauch, P., Sperner, B., Schwaiger,
    K., Gareis, M., &#38; Gottschalk, C. (2018). Endogenous microbial contamination
    of melons (<i>Cucumis melo</i>) from international trade: an underestimated risk
    for the consumer? <i>Journal of the Science of Food and Agriculture : Incorporating
    Agri-Biotech</i>, <i>98</i>(13), 5074–5081. <a href="https://doi.org/10.1002/jsfa.9045">https://doi.org/10.1002/jsfa.9045</a>'
  bjps: '<b>Esteban‐Cuesta I <i>et al.</i></b> (2018) Endogenous Microbial Contamination
    of Melons (<i>Cucumis Melo</i>) from International Trade: An Underestimated Risk
    for the Consumer? <i>Journal of the science of food and agriculture : incorporating
    Agri-Biotech</i> <b>98</b>, 5074–5081.'
  chicago: 'Esteban‐Cuesta, Irene, Nathalie Drees, Sebastian Ulrich, Peter Stauch,
    Brigitte Sperner, Karin Schwaiger, Manfred Gareis, and Christoph Gottschalk. “Endogenous
    Microbial Contamination of Melons (<i>Cucumis Melo</i>) from International Trade:
    An Underestimated Risk for the Consumer?” <i>Journal of the Science of Food and
    Agriculture : Incorporating Agri-Biotech</i> 98, no. 13 (2018): 5074–81. <a href="https://doi.org/10.1002/jsfa.9045">https://doi.org/10.1002/jsfa.9045</a>.'
  chicago-de: 'Esteban‐Cuesta, Irene, Nathalie Drees, Sebastian Ulrich, Peter Stauch,
    Brigitte Sperner, Karin Schwaiger, Manfred Gareis und Christoph Gottschalk. 2018.
    Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international
    trade: an underestimated risk for the consumer? <i>Journal of the science of food
    and agriculture : incorporating Agri-Biotech</i> 98, Nr. 13: 5074–5081. doi:<a
    href="https://doi.org/10.1002/jsfa.9045">10.1002/jsfa.9045</a>, .'
  din1505-2-1: '<span style="font-variant:small-caps;">Esteban‐Cuesta, Irene</span>
    ; <span style="font-variant:small-caps;">Drees, Nathalie</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Stauch, Peter</span>
    ; <span style="font-variant:small-caps;">Sperner, Brigitte</span> ; <span style="font-variant:small-caps;">Schwaiger,
    Karin</span> ; <span style="font-variant:small-caps;">Gareis, Manfred</span> ;
    <span style="font-variant:small-caps;">Gottschalk, Christoph</span>: Endogenous
    microbial contamination of melons (<i>Cucumis melo</i>) from international trade:
    an underestimated risk for the consumer? In: <i>Journal of the science of food
    and agriculture : incorporating Agri-Biotech</i> Bd. 98. Chichester, Wiley (2018),
    Nr. 13, S. 5074–5081'
  havard: 'I. Esteban‐Cuesta, N. Drees, S. Ulrich, P. Stauch, B. Sperner, K. Schwaiger,
    M. Gareis, C. Gottschalk, Endogenous microbial contamination of melons (<i>Cucumis
    melo</i>) from international trade: an underestimated risk for the consumer?,
    Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech. 98
    (2018) 5074–5081.'
  ieee: 'I. Esteban‐Cuesta <i>et al.</i>, “Endogenous microbial contamination of melons
    (<i>Cucumis melo</i>) from international trade: an underestimated risk for the
    consumer?,” <i>Journal of the science of food and agriculture : incorporating
    Agri-Biotech</i>, vol. 98, no. 13, pp. 5074–5081, 2018, doi: <a href="https://doi.org/10.1002/jsfa.9045">10.1002/jsfa.9045</a>.'
  mla: 'Esteban‐Cuesta, Irene, et al. “Endogenous Microbial Contamination of Melons
    (<i>Cucumis Melo</i>) from International Trade: An Underestimated Risk for the
    Consumer?” <i>Journal of the Science of Food and Agriculture : Incorporating Agri-Biotech</i>,
    vol. 98, no. 13, 2018, pp. 5074–81, <a href="https://doi.org/10.1002/jsfa.9045">https://doi.org/10.1002/jsfa.9045</a>.'
  short: 'I. Esteban‐Cuesta, N. Drees, S. Ulrich, P. Stauch, B. Sperner, K. Schwaiger,
    M. Gareis, C. Gottschalk, Journal of the Science of Food and Agriculture : Incorporating
    Agri-Biotech 98 (2018) 5074–5081.'
  ufg: '<b>Esteban‐Cuesta, Irene u. a.</b>: Endogenous microbial contamination of
    melons (<i>Cucumis melo</i>) from international trade: an underestimated risk
    for the consumer?, in: <i>Journal of the science of food and agriculture : incorporating
    Agri-Biotech</i> 98 (2018), H. 13,  S. 5074–5081.'
  van: 'Esteban‐Cuesta I, Drees N, Ulrich S, Stauch P, Sperner B, Schwaiger K, et
    al. Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international
    trade: an underestimated risk for the consumer? Journal of the science of food
    and agriculture : incorporating Agri-Biotech. 2018;98(13):5074–81.'
date_created: 2025-06-15T10:28:46Z
date_updated: 2025-06-18T11:32:30Z
department:
- _id: DEP4010
doi: 10.1002/jsfa.9045
extern: '1'
intvolume: '        98'
issue: '13'
keyword:
- foodborne pathogens
- Salmonella
- Listeria monocytogenes
- Enterobacteriaceae
- vegetables
language:
- iso: eng
page: 5074-5081
place: Chichester
publication: 'Journal of the science of food and agriculture : incorporating Agri-Biotech'
publication_identifier:
  eissn:
  - 1097-0010
  issn:
  - 0022-5142
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: 'Endogenous microbial contamination of melons (<i>Cucumis melo</i>) from international
  trade: an underestimated risk for the consumer?'
type: scientific_journal_article
user_id: '83781'
volume: 98
year: '2018'
...
---
_id: '12975'
abstract:
- lang: eng
  text: "Properly handled fish is usually marketed as “fresh fish” until day 10 after
    fishing. About 40% of the total fishery that is used for direct human consumption
    is marketed in fresh form stored at temperatures up to +2 °C. Currently, there
    are no validated methods available for controlling the recommended period of storage.
    Apart from being a potential source for food fraud, spoiled fish represents a
    major source of foodborne illnesses and intoxications.\r\nIn this study, a rapid
    MALDI-TOF mass spectrometry based screening method was developed using the vitreous
    fluid of fish eyes as specimen for the examination of different days of storage.
    The vitreous fluid was collected from n = 100 freshly fished brown trouts at day
    0, 3, 7, 9, and 11 post mortem (n = 20 brown trouts each day of examination).
    The samples were immediately measured by MALDI-TOF mass spectrometry in linear
    positive mode (mass range m/z 2000–20,000 Da). For quality assurance the experiment
    was repeated with a set of brown trouts (n = 100) originating from the same fish
    farm and with brown trouts (n = 100) originating from a different fish farm. For
    specificity testing rainbow trouts (n = 10) were examined accordingly. All obtained
    mass spectra were processed by means of MALDI Biotyper OC 3.1 and ClinProTools
    3.0 software.\r\nThe MALDI Biotyper approach showed limited applicability for
    the identification of the time of storage. However, it was suitable to reliably
    discriminate between the closely related species brown and rainbow trout. Processing
    by ClinProTools revealed four crucial mass peaks (m/z 2594 Da, m/z 4857 Da, m/z
    4879 Da, m/z 4899 Da) which enabled a reliable differentiation between day 0 and
    3, 7, 9, 11 (rate of correct identification > 90%) as well as the differentiation
    between day 3 and 7, 9, 11 (rate of correct identification > 72%). However, this
    approach showed limited applicability within the end of the tested period of storage
    when comparing between day 7, 9, or 11."
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Philipp–Michael
  full_name: Beindorf, Philipp–Michael
  last_name: Beindorf
- first_name: Barbara
  full_name: Biermaier, Barbara
  last_name: Biermaier
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
citation:
  ama: Ulrich S, Beindorf P, Biermaier B, Schwaiger K, Gareis M, Gottschalk C. A novel
    approach for the determination of freshness and identity of trouts by MALDI-TOF
    mass spectrometry. <i>Food Control</i>. 2017;80(10):281-289. doi:<a href="https://doi.org/10.1016/j.foodcont.2017.05.005">10.1016/j.foodcont.2017.05.005</a>
  apa: Ulrich, S., Beindorf, P., Biermaier, B., Schwaiger, K., Gareis, M., &#38; Gottschalk,
    C. (2017). A novel approach for the determination of freshness and identity of
    trouts by MALDI-TOF mass spectrometry. <i>Food Control</i>, <i>80</i>(10), 281–289.
    <a href="https://doi.org/10.1016/j.foodcont.2017.05.005">https://doi.org/10.1016/j.foodcont.2017.05.005</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2017) A Novel Approach for the Determination
    of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry. <i>Food Control</i>
    <b>80</b>, 281–289.
  chicago: 'Ulrich, Sebastian, Philipp–Michael Beindorf, Barbara Biermaier, Karin
    Schwaiger, Manfred Gareis, and Christoph Gottschalk. “A Novel Approach for the
    Determination of Freshness and Identity of Trouts by MALDI-TOF Mass Spectrometry.”
    <i>Food Control</i> 80, no. 10 (2017): 281–89. <a href="https://doi.org/10.1016/j.foodcont.2017.05.005">https://doi.org/10.1016/j.foodcont.2017.05.005</a>.'
  chicago-de: 'Ulrich, Sebastian, Philipp–Michael Beindorf, Barbara Biermaier, Karin
    Schwaiger, Manfred Gareis und Christoph Gottschalk. 2017. A novel approach for
    the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry.
    <i>Food Control</i> 80, Nr. 10: 281–289. doi:<a href="https://doi.org/10.1016/j.foodcont.2017.05.005">10.1016/j.foodcont.2017.05.005</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Beindorf, Philipp–Michael</span> ; <span
    style="font-variant:small-caps;">Biermaier, Barbara</span> ; <span style="font-variant:small-caps;">Schwaiger,
    Karin</span> ; <span style="font-variant:small-caps;">Gareis, Manfred</span> ;
    <span style="font-variant:small-caps;">Gottschalk, Christoph</span>: A novel approach
    for the determination of freshness and identity of trouts by MALDI-TOF mass spectrometry.
    In: <i>Food Control</i> Bd. 80. Amsterdam, Elsevier  (2017), Nr. 10, S. 281–289'
  havard: S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, C. Gottschalk,
    A novel approach for the determination of freshness and identity of trouts by
    MALDI-TOF mass spectrometry, Food Control. 80 (2017) 281–289.
  ieee: 'S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, and C. Gottschalk,
    “A novel approach for the determination of freshness and identity of trouts by
    MALDI-TOF mass spectrometry,” <i>Food Control</i>, vol. 80, no. 10, pp. 281–289,
    2017, doi: <a href="https://doi.org/10.1016/j.foodcont.2017.05.005">10.1016/j.foodcont.2017.05.005</a>.'
  mla: Ulrich, Sebastian, et al. “A Novel Approach for the Determination of Freshness
    and Identity of Trouts by MALDI-TOF Mass Spectrometry.” <i>Food Control</i>, vol.
    80, no. 10, 2017, pp. 281–89, <a href="https://doi.org/10.1016/j.foodcont.2017.05.005">https://doi.org/10.1016/j.foodcont.2017.05.005</a>.
  short: S. Ulrich, P. Beindorf, B. Biermaier, K. Schwaiger, M. Gareis, C. Gottschalk,
    Food Control 80 (2017) 281–289.
  ufg: '<b>Ulrich, Sebastian u. a.</b>: A novel approach for the determination of
    freshness and identity of trouts by MALDI-TOF mass spectrometry, in: <i>Food Control</i>
    80 (2017), H. 10,  S. 281–289.'
  van: Ulrich S, Beindorf P, Biermaier B, Schwaiger K, Gareis M, Gottschalk C. A novel
    approach for the determination of freshness and identity of trouts by MALDI-TOF
    mass spectrometry. Food Control. 2017;80(10):281–9.
date_created: 2025-06-15T10:29:56Z
date_updated: 2025-06-18T12:06:11Z
department:
- _id: DEP4010
doi: 10.1016/j.foodcont.2017.05.005
extern: '1'
intvolume: '        80'
issue: '10'
keyword:
- MALDI-TOF
- Mass spectrometry
- Freshness
- Fish
- Quality control
- Authenticity
language:
- iso: eng
page: 281-289
place: Amsterdam
publication: Food Control
publication_identifier:
  issn:
  - 0956-7135
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: A novel approach for the determination of freshness and identity of trouts
  by MALDI-TOF mass spectrometry
type: scientific_journal_article
user_id: '83781'
volume: 80
year: '2017'
...
---
_id: '12976'
abstract:
- lang: eng
  text: "The consumption of edible insects (entomophagy) will gain greater significance
    facing the increasing global population, which is suggested to reach 9 billion
    people in 2050 (FAO., 2009). Due to their high amount of proteins, fatty acids,
    vitamins, and minerals insects represent a valuable source of essential nutrients.\r\nWhile
    the consumption of insects is very common in many countries of Africa and Asia,
    there is a far smaller acceptance for entomophagy in Western cultures. Though,
    products such as noodles or burger paddies made from insect meal have a better
    compliance and can already be purchased in some countries of the European Union.
    This processing step however involves the risk of adulteration, because there
    is no more possibility to authenticate the insects once they are ground.\r\nThe
    aim of this study was to investigate whether edible insects could be measured
    and distinguished by MALDI-TOF MS (matrix-assisted laser desorption ionization-time
    of flight mass spectrometry). Therefore, different kinds of edible insects (buffalo
    worms, mealworms, crickets and grasshoppers) were purchased via online shops and
    ground subsequently. The insect powder was extracted by vigorously shaking in
    diluted formic acid and measured by MALDI-TOF MS. The measurement provided reproducible
    as well as specific mass spectra and enabled a precise differentiation of the
    different species."
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Ulrike
  full_name: Kühn, Ulrike
  last_name: Kühn
- first_name: Barbara
  full_name: Biermaier, Barbara
  last_name: Biermaier
- first_name: Nicolo
  full_name: Piacenza, Nicolo
  last_name: Piacenza
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
citation:
  ama: Ulrich S, Kühn U, Biermaier B, et al. Direct identification of edible insects
    by MALDI-TOF mass spectrometry. <i>Food Control</i>. 2017;76(6):96-101. doi:<a
    href="https://doi.org/10.1016/j.foodcont.2017.01.010">10.1016/j.foodcont.2017.01.010</a>
  apa: Ulrich, S., Kühn, U., Biermaier, B., Piacenza, N., Schwaiger, K., Gottschalk,
    C., &#38; Gareis, M. (2017). Direct identification of edible insects by MALDI-TOF
    mass spectrometry. <i>Food Control</i>, <i>76</i>(6), 96–101. <a href="https://doi.org/10.1016/j.foodcont.2017.01.010">https://doi.org/10.1016/j.foodcont.2017.01.010</a>
  bjps: <b>Ulrich S <i>et al.</i></b> (2017) Direct Identification of Edible Insects
    by MALDI-TOF Mass Spectrometry. <i>Food Control</i> <b>76</b>, 96–101.
  chicago: 'Ulrich, Sebastian, Ulrike Kühn, Barbara Biermaier, Nicolo Piacenza, Karin
    Schwaiger, Christoph Gottschalk, and Manfred Gareis. “Direct Identification of
    Edible Insects by MALDI-TOF Mass Spectrometry.” <i>Food Control</i> 76, no. 6
    (2017): 96–101. <a href="https://doi.org/10.1016/j.foodcont.2017.01.010">https://doi.org/10.1016/j.foodcont.2017.01.010</a>.'
  chicago-de: 'Ulrich, Sebastian, Ulrike Kühn, Barbara Biermaier, Nicolo Piacenza,
    Karin Schwaiger, Christoph Gottschalk und Manfred Gareis. 2017. Direct identification
    of edible insects by MALDI-TOF mass spectrometry. <i>Food Control</i> 76, Nr.
    6: 96–101. doi:<a href="https://doi.org/10.1016/j.foodcont.2017.01.010">10.1016/j.foodcont.2017.01.010</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;">Ulrich, Sebastian</span> ;
    <span style="font-variant:small-caps;">Kühn, Ulrike</span> ; <span style="font-variant:small-caps;">Biermaier,
    Barbara</span> ; <span style="font-variant:small-caps;">Piacenza, Nicolo</span>
    ; <span style="font-variant:small-caps;">Schwaiger, Karin</span> ; <span style="font-variant:small-caps;">Gottschalk,
    Christoph</span> ; <span style="font-variant:small-caps;">Gareis, Manfred</span>:
    Direct identification of edible insects by MALDI-TOF mass spectrometry. In: <i>Food
    Control</i> Bd. 76. Amsterdam, Elsevier (2017), Nr. 6, S. 96–101'
  havard: S. Ulrich, U. Kühn, B. Biermaier, N. Piacenza, K. Schwaiger, C. Gottschalk,
    M. Gareis, Direct identification of edible insects by MALDI-TOF mass spectrometry,
    Food Control. 76 (2017) 96–101.
  ieee: 'S. Ulrich <i>et al.</i>, “Direct identification of edible insects by MALDI-TOF
    mass spectrometry,” <i>Food Control</i>, vol. 76, no. 6, pp. 96–101, 2017, doi:
    <a href="https://doi.org/10.1016/j.foodcont.2017.01.010">10.1016/j.foodcont.2017.01.010</a>.'
  mla: Ulrich, Sebastian, et al. “Direct Identification of Edible Insects by MALDI-TOF
    Mass Spectrometry.” <i>Food Control</i>, vol. 76, no. 6, 2017, pp. 96–101, <a
    href="https://doi.org/10.1016/j.foodcont.2017.01.010">https://doi.org/10.1016/j.foodcont.2017.01.010</a>.
  short: S. Ulrich, U. Kühn, B. Biermaier, N. Piacenza, K. Schwaiger, C. Gottschalk,
    M. Gareis, Food Control 76 (2017) 96–101.
  ufg: '<b>Ulrich, Sebastian u. a.</b>: Direct identification of edible insects by
    MALDI-TOF mass spectrometry, in: <i>Food Control</i> 76 (2017), H. 6,  S. 96–101.'
  van: Ulrich S, Kühn U, Biermaier B, Piacenza N, Schwaiger K, Gottschalk C, et al.
    Direct identification of edible insects by MALDI-TOF mass spectrometry. Food Control.
    2017;76(6):96–101.
date_created: 2025-06-15T10:31:37Z
date_updated: 2025-06-18T12:08:47Z
department:
- _id: DEP4010
doi: 10.1016/j.foodcont.2017.01.010
extern: '1'
intvolume: '        76'
issue: '6'
keyword:
- MALDI-TOF MS
- Mass spectrometry
- Edible insects
- Authenticity
- Food quality
language:
- iso: eng
page: 96-101
place: Amsterdam
publication: Food Control
publication_identifier:
  issn:
  - 0956-7135
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Direct identification of edible insects by MALDI-TOF mass spectrometry
type: scientific_journal_article
user_id: '83781'
volume: 76
year: '2017'
...
---
_id: '12977'
abstract:
- lang: eng
  text: 'Stachybotrys (S.) spp. are omnipresent cellulolytic molds. Some species are
    highly toxic owing to their ability to synthesize various secondary metabolites
    such as macrocyclic trichothecenes or hemolysins. The reliable identification
    of Stachybotrys at species level is currently limited to genome-based identification.
    This study aimed to establish a fast and reliable MALDI-TOF MS identification
    method by optimizing the pre-analytical steps for protein extraction for subsequent
    generation of high-quality fingerprint mass spectra. Eight reference strains of
    the American Type Culture Collection and the Technical University of Denmark were
    cultivated in triplicate (biological repetitions) for 2 days in malt extract broth.
    The mycelia (1.5 ml) were first washed with 75 % ethanol and an additional washing
    step with dimethyl sulfoxide (10 %) was added to remove unspecific low weight
    masses. Furthermore, mycelia were broken with roughened glass beads in formic
    acid (70 %) and acetonitrile. The method was successfully applied to a total of
    45 isolates of Stachybotrys originating from three different habitats (indoor,
    feed, and food samples; n = 15 each): Twenty-seven isolates of S. chartarum and
    18 isolates of S. chlorohalonata could be identified by MALDI-TOF MS. The data
    obtained exactly matched those obtained by genome-based identification. The mean
    score values for S. chartarum ranged from 2.509 to 2.739 and from 2.148 to 2.622
    for S. chlorohalonata with a very good reproducibility: the relative standard
    deviations were between 0.3 % and 6.8 %. Thus, MALDI-TOF MS proved to be a fast
    and reliable alternative to identification of Stachybotrys spp. by nucleotide
    amplification and sequencing.'
author:
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Barbara
  full_name: Biermaier, Barbara
  last_name: Biermaier
- first_name: Oliver
  full_name: Bader, Oliver
  last_name: Bader
- first_name: Georg
  full_name: Wolf, Georg
  last_name: Wolf
- first_name: Reinhard K.
  full_name: Straubinger, Reinhard K.
  last_name: Straubinger
- first_name: Andrea
  full_name: Didier, Andrea
  last_name: Didier
- first_name: Brigitte
  full_name: Sperner, Brigitte
  last_name: Sperner
- first_name: Karin
  full_name: Schwaiger, Karin
  last_name: Schwaiger
- first_name: Manfred
  full_name: Gareis, Manfred
  last_name: Gareis
- first_name: Christoph
  full_name: Gottschalk, Christoph
  last_name: Gottschalk
citation:
  ama: 'Ulrich S, Biermaier B, Bader O, et al. Identification of Stachybotrys spp.
    by MALDI-TOF mass spectrometry. <i>  Analytical &#38; bioanalytical chemistry :
    a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i>.
    2016;408(27):7565-7581. doi:<a href="https://doi.org/10.1007/s00216-016-9800-9">10.1007/s00216-016-9800-9</a>'
  apa: 'Ulrich, S., Biermaier, B., Bader, O., Wolf, G., Straubinger, R. K., Didier,
    A., Sperner, B., Schwaiger, K., Gareis, M., &#38; Gottschalk, C. (2016). Identification
    of Stachybotrys spp. by MALDI-TOF mass spectrometry. <i>  Analytical &#38; Bioanalytical
    Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and
    Quimica Analitica</i>, <i>408</i>(27), 7565–7581. <a href="https://doi.org/10.1007/s00216-016-9800-9">https://doi.org/10.1007/s00216-016-9800-9</a>'
  bjps: '<b>Ulrich S <i>et al.</i></b> (2016) Identification of Stachybotrys Spp.
    by MALDI-TOF Mass Spectrometry. <i>  Analytical &#38; bioanalytical chemistry :
    a merger of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i>
    <b>408</b>, 7565–7581.'
  chicago: 'Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard
    K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis,
    and Christoph Gottschalk. “Identification of Stachybotrys Spp. by MALDI-TOF Mass
    Spectrometry.” <i>  Analytical &#38; Bioanalytical Chemistry : A Merger of Fresenius’
    Journal of Analytical Chemistry, Analusis and Quimica Analitica</i> 408, no. 27
    (2016): 7565–81. <a href="https://doi.org/10.1007/s00216-016-9800-9">https://doi.org/10.1007/s00216-016-9800-9</a>.'
  chicago-de: 'Ulrich, Sebastian, Barbara Biermaier, Oliver Bader, Georg Wolf, Reinhard
    K. Straubinger, Andrea Didier, Brigitte Sperner, Karin Schwaiger, Manfred Gareis
    und Christoph Gottschalk. 2016. Identification of Stachybotrys spp. by MALDI-TOF
    mass spectrometry. <i>  Analytical &#38; bioanalytical chemistry : a merger of
    Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i>
    408, Nr. 27: 7565–7581. doi:<a href="https://doi.org/10.1007/s00216-016-9800-9">10.1007/s00216-016-9800-9</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Biermaier, Barbara</span>
    ; <span style="font-variant:small-caps;">Bader, Oliver</span> ; <span style="font-variant:small-caps;">Wolf,
    Georg</span> ; <span style="font-variant:small-caps;">Straubinger, Reinhard K.</span>
    ; <span style="font-variant:small-caps;">Didier, Andrea</span> ; <span style="font-variant:small-caps;">Sperner,
    Brigitte</span> ; <span style="font-variant:small-caps;">Schwaiger, Karin</span>
    ; u. a.</span>: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry.
    In: <i>  Analytical &#38; bioanalytical chemistry : a merger of Fresenius’ journal
    of analytical chemistry, Analusis and Quimica analitica</i> Bd. 408. Berlin ;
    Heidelberg, Springer (2016), Nr. 27, S. 7565–7581'
  havard: 'S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier,
    B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk, Identification of Stachybotrys
    spp. by MALDI-TOF mass spectrometry,   Analytical &#38; Bioanalytical Chemistry :
    A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica.
    408 (2016) 7565–7581.'
  ieee: 'S. Ulrich <i>et al.</i>, “Identification of Stachybotrys spp. by MALDI-TOF
    mass spectrometry,” <i>  Analytical &#38; bioanalytical chemistry : a merger of
    Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i>,
    vol. 408, no. 27, pp. 7565–7581, 2016, doi: <a href="https://doi.org/10.1007/s00216-016-9800-9">10.1007/s00216-016-9800-9</a>.'
  mla: 'Ulrich, Sebastian, et al. “Identification of Stachybotrys Spp. by MALDI-TOF
    Mass Spectrometry.” <i>  Analytical &#38; Bioanalytical Chemistry : A Merger of
    Fresenius’ Journal of Analytical Chemistry, Analusis and Quimica Analitica</i>,
    vol. 408, no. 27, 2016, pp. 7565–81, <a href="https://doi.org/10.1007/s00216-016-9800-9">https://doi.org/10.1007/s00216-016-9800-9</a>.'
  short: 'S. Ulrich, B. Biermaier, O. Bader, G. Wolf, R.K. Straubinger, A. Didier,
    B. Sperner, K. Schwaiger, M. Gareis, C. Gottschalk,   Analytical &#38; Bioanalytical
    Chemistry : A Merger of Fresenius’ Journal of Analytical Chemistry, Analusis and
    Quimica Analitica 408 (2016) 7565–7581.'
  ufg: '<b>Ulrich, Sebastian u. a.</b>: Identification of Stachybotrys spp. by MALDI-TOF
    mass spectrometry, in: <i>  Analytical &#38; bioanalytical chemistry : a merger
    of Fresenius’ journal of analytical chemistry, Analusis and Quimica analitica</i>
    408 (2016), H. 27,  S. 7565–7581.'
  van: 'Ulrich S, Biermaier B, Bader O, Wolf G, Straubinger RK, Didier A, et al. Identification
    of Stachybotrys spp. by MALDI-TOF mass spectrometry.   Analytical &#38; bioanalytical
    chemistry : a merger of Fresenius’ journal of analytical chemistry, Analusis and
    Quimica analitica. 2016;408(27):7565–81.'
date_created: 2025-06-15T10:32:52Z
date_updated: 2025-06-18T12:11:46Z
department:
- _id: DEP4010
doi: 10.1007/s00216-016-9800-9
extern: '1'
intvolume: '       408'
issue: '27'
keyword:
- Stachybotrys spp
- MALDI-TOF MS
- Mass spectrometry
- Filamentous fungi
language:
- iso: eng
page: 7565-7581
place: Berlin ; Heidelberg
publication: '  Analytical & bioanalytical chemistry : a merger of Fresenius'' journal
  of analytical chemistry, Analusis and Quimica analitica'
publication_identifier:
  eissn:
  - 1618-2650
  issn:
  - 1618-2642
publication_status: published
publisher: Springer
quality_controlled: '1'
status: public
title: Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry
type: scientific_journal_article
user_id: '83781'
volume: 408
year: '2016'
...
---
_id: '12978'
abstract:
- lang: eng
  text: Diet change and fatness are supposed to challenge the immune system of the
    cow. Therefore, immunological and haematological consequences of adaptation to
    and continued feeding of a high-energy diet were studied in eight non-pregnant,
    non-lactating Holstein cows over 16 weeks. Blood haptoglobin concentration remained
    unaltered, suggesting that an acute phase reaction was not induced. Stimulation
    ability of peripheral blood mononuclear cells and stimulated oxidative burst capacity
    of granulocytes increased significantly in the course of the experiment after
    an initial drop. While total leucocyte counts increased, the proportion of granulocytes
    increased and that of lymphocytes decreased at the same time as the ratio of CD4+/CD8+
    lymphocytes did. Capability of rumen microbes to detoxify the immune-modulating
    mycotoxin deoxynivalenol (DON) was not compromised as indicated by the exclusive
    presence of de-DON as the detoxified DON metabolite in blood. In conclusion, both
    diet change and prolonged positive energy balance influenced the bovine immune
    system.
author:
- first_name: Sven
  full_name: Dänicke, Sven
  last_name: Dänicke
- first_name: Ulrich
  full_name: Meyer, Ulrich
  last_name: Meyer
- first_name: Janine
  full_name: Winkler, Janine
  last_name: Winkler
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Jana
  full_name: Frahm, Jana
  last_name: Frahm
- first_name: Susanne
  full_name: Kersten, Susanne
  last_name: Kersten
- first_name: Hana
  full_name: Valenta, Hana
  last_name: Valenta
- first_name: Jürgen
  full_name: Rehage, Jürgen
  last_name: Rehage
- first_name: Susanne
  full_name: Häussler, Susanne
  last_name: Häussler
- first_name: Helga
  full_name: Sauerwein, Helga
  last_name: Sauerwein
- first_name: Lena
  full_name: Locher, Lena
  last_name: Locher
citation:
  ama: Dänicke S, Meyer U, Winkler J, et al. Haematological and immunological adaptations
    of non-pregnant, non-lactating dairy cows to a high-energetic diet containing
    mycotoxins. <i>Archives of Animal Nutrition</i>. 2015;70(1):1-16. doi:<a href="https://doi.org/10.1080/1745039x.2015.1117561">10.1080/1745039x.2015.1117561</a>
  apa: Dänicke, S., Meyer, U., Winkler, J., Ulrich, S., Frahm, J., Kersten, S., Valenta,
    H., Rehage, J., Häussler, S., Sauerwein, H., &#38; Locher, L. (2015). Haematological
    and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic
    diet containing mycotoxins. <i>Archives of Animal Nutrition</i>, <i>70</i>(1),
    1–16. <a href="https://doi.org/10.1080/1745039x.2015.1117561">https://doi.org/10.1080/1745039x.2015.1117561</a>
  bjps: <b>Dänicke S <i>et al.</i></b> (2015) Haematological and Immunological Adaptations
    of Non-Pregnant, Non-Lactating Dairy Cows to a High-Energetic Diet Containing
    Mycotoxins. <i>Archives of Animal Nutrition</i> <b>70</b>, 1–16.
  chicago: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Sebastian Ulrich, Jana Frahm,
    Susanne Kersten, Hana Valenta, et al. “Haematological and Immunological Adaptations
    of Non-Pregnant, Non-Lactating Dairy Cows to a High-Energetic Diet Containing
    Mycotoxins.” <i>Archives of Animal Nutrition</i> 70, no. 1 (2015): 1–16. <a href="https://doi.org/10.1080/1745039x.2015.1117561">https://doi.org/10.1080/1745039x.2015.1117561</a>.'
  chicago-de: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Sebastian Ulrich, Jana
    Frahm, Susanne Kersten, Hana Valenta, u. a. 2015. Haematological and immunological
    adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet
    containing mycotoxins. <i>Archives of Animal Nutrition</i> 70, Nr. 1: 1–16. doi:<a
    href="https://doi.org/10.1080/1745039x.2015.1117561">10.1080/1745039x.2015.1117561</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Dänicke,
    Sven</span> ; <span style="font-variant:small-caps;">Meyer, Ulrich</span> ; <span
    style="font-variant:small-caps;">Winkler, Janine</span> ; <span style="font-variant:small-caps;">Ulrich,
    Sebastian</span> ; <span style="font-variant:small-caps;">Frahm, Jana</span> ;
    <span style="font-variant:small-caps;">Kersten, Susanne</span> ; <span style="font-variant:small-caps;">Valenta,
    Hana</span> ; <span style="font-variant:small-caps;">Rehage, Jürgen</span> ; u. a.</span>:
    Haematological and immunological adaptations of non-pregnant, non-lactating dairy
    cows to a high-energetic diet containing mycotoxins. In: <i>Archives of Animal
    Nutrition</i> Bd. 70. Abingdon, Taylor &#38; Francis (2015), Nr. 1, S. 1–16'
  havard: S. Dänicke, U. Meyer, J. Winkler, S. Ulrich, J. Frahm, S. Kersten, H. Valenta,
    J. Rehage, S. Häussler, H. Sauerwein, L. Locher, Haematological and immunological
    adaptations of non-pregnant, non-lactating dairy cows to a high-energetic diet
    containing mycotoxins, Archives of Animal Nutrition. 70 (2015) 1–16.
  ieee: 'S. Dänicke <i>et al.</i>, “Haematological and immunological adaptations of
    non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins,”
    <i>Archives of Animal Nutrition</i>, vol. 70, no. 1, pp. 1–16, 2015, doi: <a href="https://doi.org/10.1080/1745039x.2015.1117561">10.1080/1745039x.2015.1117561</a>.'
  mla: Dänicke, Sven, et al. “Haematological and Immunological Adaptations of Non-Pregnant,
    Non-Lactating Dairy Cows to a High-Energetic Diet Containing Mycotoxins.” <i>Archives
    of Animal Nutrition</i>, vol. 70, no. 1, 2015, pp. 1–16, <a href="https://doi.org/10.1080/1745039x.2015.1117561">https://doi.org/10.1080/1745039x.2015.1117561</a>.
  short: S. Dänicke, U. Meyer, J. Winkler, S. Ulrich, J. Frahm, S. Kersten, H. Valenta,
    J. Rehage, S. Häussler, H. Sauerwein, L. Locher, Archives of Animal Nutrition
    70 (2015) 1–16.
  ufg: '<b>Dänicke, Sven u. a.</b>: Haematological and immunological adaptations of
    non-pregnant, non-lactating dairy cows to a high-energetic diet containing mycotoxins,
    in: <i>Archives of Animal Nutrition</i> 70 (2015), H. 1,  S. 1–16.'
  van: Dänicke S, Meyer U, Winkler J, Ulrich S, Frahm J, Kersten S, et al. Haematological
    and immunological adaptations of non-pregnant, non-lactating dairy cows to a high-energetic
    diet containing mycotoxins. Archives of Animal Nutrition. 2015;70(1):1–16.
date_created: 2025-06-15T10:34:57Z
date_updated: 2025-06-18T12:18:46Z
department:
- _id: DEP4010
doi: 10.1080/1745039x.2015.1117561
extern: '1'
intvolume: '        70'
issue: '1'
keyword:
- Dairy cowsdeoxynivalenol
- energy consumption
- functional responses
- haematology
- leucocytes
- mycotoxins
- zearalenone
language:
- iso: eng
page: 1-16
place: Abingdon
publication: Archives of Animal Nutrition
publication_identifier:
  eissn:
  - 1477-2817
  issn:
  - 1745-039X
  unknown:
  - '0003-942X '
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
status: public
title: Haematological and immunological adaptations of non-pregnant, non-lactating
  dairy cows to a high-energetic diet containing mycotoxins
type: scientific_journal_article
user_id: '83781'
volume: 70
year: '2015'
...
---
_id: '12979'
abstract:
- lang: eng
  text: Physiological consequences of adaptation to and continued feeding of a high-energetic
    diet were studied in eight non-pregnant, non-lactating dairy Holstein cows over
    a period of 16 weeks. The first six weeks served as an adaptation period from
    the low energetic straw-based diet (3.8 MJ NEL/kg DM) to the high-energetic ration
    (7.5 MJ NEL/kg DM). Intake of dry matter (DM) increased with dietary energy concentration
    from 9 to 20 kg/d up to week 9 to 12 and decreased thereafter. The initial live
    weight (LW) of 550 ± 60 kg was increased linearly and corresponded to an average
    daily LW gain of 2.3 ± 0.3 kg. Energy balance increased approximately nine-fold
    to a maximum of 114 MJ NEL/d in week 10. Ruminal fermentation pattern was completely
    changed from an acetate dominating profile to a propionate based one, which was
    paralleled by a marked increase in the rumen fluid endotoxin concentration. Unlike
    blood glucose concentration, which increased continuously, that of cholesterol
    and triglycerides started to increase after an initial stagnation. In conclusion,
    both ruminal adaptation to a high-energetic diet and the continued feeding of
    such a diet induced digestive and metabolic adaptations in non-pregnant, non-lactating
    cows characterised by a progressing positive energy balance.
author:
- first_name: Sven
  full_name: Dänicke, Sven
  last_name: Dänicke
- first_name: Ulrich
  full_name: Meyer, Ulrich
  last_name: Meyer
- first_name: Janine
  full_name: Winkler, Janine
  last_name: Winkler
- first_name: Kirsten
  full_name: Schulz, Kirsten
  last_name: Schulz
- first_name: Sebastian
  full_name: Ulrich, Sebastian
  id: '85847'
  last_name: Ulrich
  orcid: 0000-0002-4511-9537
- first_name: Jana
  full_name: Frahm, Jana
  last_name: Frahm
- first_name: Susanne
  full_name: Kersten, Susanne
  last_name: Kersten
- first_name: Jürgen
  full_name: Rehage, Jürgen
  last_name: Rehage
- first_name: Gerhard
  full_name: Breves, Gerhard
  last_name: Breves
- first_name: Susanne
  full_name: Häußler, Susanne
  last_name: Häußler
- first_name: Helga
  full_name: Sauerwein, Helga
  last_name: Sauerwein
- first_name: Lena
  full_name: Locher, Lena
  last_name: Locher
citation:
  ama: Dänicke S, Meyer U, Winkler J, et al. Description of a bovine model for studying
    digestive and metabolic effects of a positive energy balance not biased by lactation
    or gravidity. <i>Archives of Animal Nutrition</i>. 2014;68(6):460-477. doi:<a
    href="https://doi.org/10.1080/1745039x.2014.973243">10.1080/1745039x.2014.973243</a>
  apa: Dänicke, S., Meyer, U., Winkler, J., Schulz, K., Ulrich, S., Frahm, J., Kersten,
    S., Rehage, J., Breves, G., Häußler, S., Sauerwein, H., &#38; Locher, L. (2014).
    Description of a bovine model for studying digestive and metabolic effects of
    a positive energy balance not biased by lactation or gravidity. <i>Archives of
    Animal Nutrition</i>, <i>68</i>(6), 460–477. <a href="https://doi.org/10.1080/1745039x.2014.973243">https://doi.org/10.1080/1745039x.2014.973243</a>
  bjps: <b>Dänicke S <i>et al.</i></b> (2014) Description of a Bovine Model for Studying
    Digestive and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation
    or Gravidity. <i>Archives of Animal Nutrition</i> <b>68</b>, 460–477.
  chicago: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian
    Ulrich, Jana Frahm, Susanne Kersten, et al. “Description of a Bovine Model for
    Studying Digestive and Metabolic Effects of a Positive Energy Balance Not Biased
    by Lactation or Gravidity.” <i>Archives of Animal Nutrition</i> 68, no. 6 (2014):
    460–77. <a href="https://doi.org/10.1080/1745039x.2014.973243">https://doi.org/10.1080/1745039x.2014.973243</a>.'
  chicago-de: 'Dänicke, Sven, Ulrich Meyer, Janine Winkler, Kirsten Schulz, Sebastian
    Ulrich, Jana Frahm, Susanne Kersten, u. a. 2014. Description of a bovine model
    for studying digestive and metabolic effects of a positive energy balance not
    biased by lactation or gravidity. <i>Archives of Animal Nutrition</i> 68, Nr.
    6: 460–477. doi:<a href="https://doi.org/10.1080/1745039x.2014.973243">10.1080/1745039x.2014.973243</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Dänicke,
    Sven</span> ; <span style="font-variant:small-caps;">Meyer, Ulrich</span> ; <span
    style="font-variant:small-caps;">Winkler, Janine</span> ; <span style="font-variant:small-caps;">Schulz,
    Kirsten</span> ; <span style="font-variant:small-caps;">Ulrich, Sebastian</span>
    ; <span style="font-variant:small-caps;">Frahm, Jana</span> ; <span style="font-variant:small-caps;">Kersten,
    Susanne</span> ; <span style="font-variant:small-caps;">Rehage, Jürgen</span>
    ; u. a.</span>: Description of a bovine model for studying digestive and metabolic
    effects of a positive energy balance not biased by lactation or gravidity. In:
    <i>Archives of Animal Nutrition</i> Bd. 68. Abingdon, Taylor &#38; Francis (2014),
    Nr. 6, S. 460–477'
  havard: S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten,
    J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Description of a bovine
    model for studying digestive and metabolic effects of a positive energy balance
    not biased by lactation or gravidity, Archives of Animal Nutrition. 68 (2014)
    460–477.
  ieee: 'S. Dänicke <i>et al.</i>, “Description of a bovine model for studying digestive
    and metabolic effects of a positive energy balance not biased by lactation or
    gravidity,” <i>Archives of Animal Nutrition</i>, vol. 68, no. 6, pp. 460–477,
    2014, doi: <a href="https://doi.org/10.1080/1745039x.2014.973243">10.1080/1745039x.2014.973243</a>.'
  mla: Dänicke, Sven, et al. “Description of a Bovine Model for Studying Digestive
    and Metabolic Effects of a Positive Energy Balance Not Biased by Lactation or
    Gravidity.” <i>Archives of Animal Nutrition</i>, vol. 68, no. 6, 2014, pp. 460–77,
    <a href="https://doi.org/10.1080/1745039x.2014.973243">https://doi.org/10.1080/1745039x.2014.973243</a>.
  short: S. Dänicke, U. Meyer, J. Winkler, K. Schulz, S. Ulrich, J. Frahm, S. Kersten,
    J. Rehage, G. Breves, S. Häußler, H. Sauerwein, L. Locher, Archives of Animal
    Nutrition 68 (2014) 460–477.
  ufg: '<b>Dänicke, Sven u. a.</b>: Description of a bovine model for studying digestive
    and metabolic effects of a positive energy balance not biased by lactation or
    gravidity, in: <i>Archives of Animal Nutrition</i> 68 (2014), H. 6,  S. 460–477.'
  van: Dänicke S, Meyer U, Winkler J, Schulz K, Ulrich S, Frahm J, et al. Description
    of a bovine model for studying digestive and metabolic effects of a positive energy
    balance not biased by lactation or gravidity. Archives of Animal Nutrition. 2014;68(6):460–77.
date_created: 2025-06-15T10:36:14Z
date_updated: 2025-06-18T12:23:01Z
department:
- _id: DEP4010
doi: 10.1080/1745039x.2014.973243
extern: '1'
intvolume: '        68'
issue: '6'
keyword:
- blood chemistry
- dairy cows
- endotoxins
- energy balance
- energy content
- rumen fermentation
language:
- iso: eng
page: 460-477
place: Abingdon
publication: Archives of Animal Nutrition
publication_identifier:
  eissn:
  - 1477-2817
  issn:
  - 1745-039X
  - 0003-942X
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
status: public
title: Description of a bovine model for studying digestive and metabolic effects
  of a positive energy balance not biased by lactation or gravidity
type: scientific_journal_article
user_id: '83781'
volume: 68
year: '2014'
...