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Further motivations of initiators and supporters to join crowdfunding campaigns are getting illuminated. Factors of success for crowdfunding campaigns get identified. After literature research three example projects get analysed and discussed regarding the factors of success. Crowdfunding was found to be a chance to finance student films with low budgets. 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While they hold some advantages like stronger immersion, also side effects like visual fatigue were reported. One of the reasons for this is the vergence-accomodation conflict, which describes the unnatural uncoupling of vergence and accomodation that audiences of stereoscopic films are forced to do. As this conflict only causes symptoms at excessive discrepancies, filmmakers should keep the stereoscopic disparity in their films below a certain level. However, there is no consensus on what level that limit should be, so a general guideline is needed. By investigating the findings of different authors and analysing the disparity values of five stereoscopic feature films, a recommendation to limit the disparity of theatrical films to a value of 3% in negative space and 2% in positive space is given."}],"date_created":"2019-04-16T09:39:15Z","place":"Lemgo","status":"public","page":"36","citation":{"ieee":"M. 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Lemgo: Hochschule Ostwestfalen-Lippe.","ieee":"N. Bauerschaefer, <i>Golden Shire</i>. Lemgo: Hochschule Ostwestfalen-Lippe, 2012.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Bauerschaefer, Nico</span>: <i>Golden Shire</i>. Lemgo : Hochschule Ostwestfalen-Lippe, 2012","havard":"N. Bauerschaefer, Golden Shire, Hochschule Ostwestfalen-Lippe, Lemgo, 2012.","short":"N. Bauerschaefer, Golden Shire, Hochschule Ostwestfalen-Lippe, Lemgo, 2012.","mla":"Bauerschaefer, Nico. <i>Golden Shire</i>. Hochschule Ostwestfalen-Lippe, 2012.","apa":"Bauerschaefer, N. (2012). <i>Golden Shire</i>. Lemgo: Hochschule Ostwestfalen-Lippe.","van":"Bauerschaefer N. Golden Shire. Lemgo: Hochschule Ostwestfalen-Lippe; 2012. 46 p.","ama":"Bauerschaefer N. <i>Golden Shire</i>. Lemgo: Hochschule Ostwestfalen-Lippe; 2012.","ufg":"<b>Bauerschaefer, Nico (2012)</b>: Golden Shire, Lemgo.","chicago-de":"Bauerschaefer, Nico. 2012. <i>Golden Shire</i>. 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Hochschule Ostwestfalen-Lippe, 2012.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Herting, Andreas</span>: <i>Erlebe dein Wissen! : Konzeption und Neugestaltung der Avendoo Website</i>. Lemgo : Hochschule Ostwestfalen-Lippe, 2012","havard":"A. Herting, Erlebe dein Wissen! : Konzeption und Neugestaltung der Avendoo Website, Hochschule Ostwestfalen-Lippe, Lemgo, 2012.","bjps":"<b>Herting A</b> (2012) <i>Erlebe dein Wissen! : Konzeption und Neugestaltung der Avendoo Website</i>. Lemgo: Hochschule Ostwestfalen-Lippe.","ieee":"A. Herting, <i>Erlebe dein Wissen! : Konzeption und Neugestaltung der Avendoo Website</i>. Lemgo: Hochschule Ostwestfalen-Lippe, 2012."},"page":"132","has_accepted_license":"1","supervisor":[{"last_name":"Baudeck","first_name":"Stefan","full_name":"Baudeck, Stefan"},{"last_name":"Lechtenberg","id":"10784","first_name":"Frank","full_name":"Lechtenberg, Frank"}],"date_updated":"2023-03-15T13:49:34Z","type":"bachelor_thesis","file_date_updated":"2019-04-17T08:13:22Z","user_id":"15514","_id":"1063","keyword":["Gestaltung","Konzeption","Web Site"]},{"citation":{"van":"Nemcik T. Produktion eines Trailers für ein Buch. Lemgo: Hochschule Ostwestfalen-Lippe; 2012. 30 p.","mla":"Nemcik, Thomas. <i>Produktion eines Trailers für ein Buch</i>. Hochschule Ostwestfalen-Lippe, 2012.","apa":"Nemcik, T. (2012). <i>Produktion eines Trailers für ein Buch</i>. Lemgo: Hochschule Ostwestfalen-Lippe.","short":"T. Nemcik, Produktion eines Trailers für ein Buch, Hochschule Ostwestfalen-Lippe, Lemgo, 2012.","havard":"T. Nemcik, Produktion eines Trailers für ein Buch, Hochschule Ostwestfalen-Lippe, Lemgo, 2012.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Nemcik, Thomas</span>: <i>Produktion eines Trailers für ein Buch</i>. Lemgo : Hochschule Ostwestfalen-Lippe, 2012","ieee":"T. Nemcik, <i>Produktion eines Trailers für ein Buch</i>. Lemgo: Hochschule Ostwestfalen-Lippe, 2012.","bjps":"<b>Nemcik T</b> (2012) <i>Produktion eines Trailers für ein Buch</i>. Lemgo: Hochschule Ostwestfalen-Lippe.","chicago":"Nemcik, Thomas. <i>Produktion eines Trailers für ein Buch</i>. Lemgo: Hochschule Ostwestfalen-Lippe, 2012.","chicago-de":"Nemcik, Thomas. 2012. <i>Produktion eines Trailers für ein Buch</i>. Lemgo: Hochschule Ostwestfalen-Lippe.","ufg":"<b>Nemcik, Thomas (2012)</b>: Produktion eines Trailers für ein Buch, Lemgo.","ama":"Nemcik T. <i>Produktion eines Trailers für ein Buch</i>. Lemgo: Hochschule Ostwestfalen-Lippe; 2012."},"page":"30","status":"public","place":"Lemgo","ddc":["791"],"file_date_updated":"2019-04-17T08:20:04Z","keyword":["Kriminalgeschichte","Trailer <Film>"],"_id":"1065","supervisor":[{"last_name":"Lechtenberg","id":"10784","first_name":"Frank","full_name":"Lechtenberg, Frank"}],"date_created":"2019-04-17T08:16:09Z","publisher":"Hochschule Ostwestfalen-Lippe","file":[{"file_id":"1066","content_type":"application/pdf","date_created":"2019-04-17T08:20:04Z","relation":"main_file","creator":"hemke","access_level":"local","date_updated":"2019-04-17T08:20:04Z","file_size":7748866,"file_name":"Bachelorarbeit_Thomas_Nemcik.pdf"}],"abstract":[{"lang":"ger","text":"Die zwei Autorinnen waren auf der Suche nach jemanden, der entweder mit ihnen zusammen oder alleine einen kurzen Buchtrailer für ihren Ende Februar erscheinenden Debütroman Die Muse des Mörders produzieren könnte, um diesen etwas bekannter zu machen. Eine grobe Idee für den Trailer war schon da, das ganze sollte allerdings passend zum Erscheinen des Buches fertig sein und konnte von ihrer Seite aus nicht finanziell unterstützt werden. Nach weiteren Absprachen konnten folgende Rahmenbedingungen festgehalten werden: • Trailer für einen Roman • Genre: Thriller/Krimi • Dauer: 30 bis 120 Sekunden • finanziell eingeschränkte Produktion • soll nicht zu viele Details enthalten"}],"department":[{"_id":"DEP2001"}],"language":[{"iso":"ger"}],"year":2012,"author":[{"full_name":"Nemcik, Thomas","first_name":"Thomas","last_name":"Nemcik"}],"title":"Produktion eines Trailers für ein Buch","type":"bachelor_thesis","user_id":"15514","date_updated":"2023-03-15T13:49:34Z","has_accepted_license":"1"},{"date_updated":"2024-11-19T10:42:17Z","conference":{"location":"Istanbul (Türkei)","end_date":"2012-03-22","start_date":"2012-03-19","name":"8th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology"},"_id":"12041","user_id":"83781","type":"conference_scientific_abstract","author":[{"full_name":"Kamke, Dominic","first_name":"Dominic","id":"82841","last_name":"Kamke"},{"first_name":"A.","last_name":"Bennyassine","full_name":"Bennyassine, A."},{"full_name":"Kutz, Gerd","first_name":"Gerd","id":"12015","last_name":"Kutz"},{"full_name":"Meier, S.","last_name":"Meier","first_name":"S."}],"title":"Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) using two Coating Formulations","language":[{"iso":"eng"}],"department":[{"_id":"DEP4022"}],"year":"2012","publication_status":"published","citation":{"chicago-de":"Kamke, Dominic, A. Bennyassine, Gerd Kutz und S. Meier. 2012. <i>Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) using two Coating Formulations</i>.","chicago":"Kamke, Dominic, A. Bennyassine, Gerd Kutz, and S. Meier. <i>Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) Using Two Coating Formulations</i>, 2012.","ufg":"<b>Kamke, Dominic u. a.</b>: Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) using two Coating Formulations, o. O. 2012.","ama":"Kamke D, Bennyassine A, Kutz G, Meier S. <i>Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) Using Two Coating Formulations</i>.; 2012.","havard":"D. Kamke, A. Bennyassine, G. Kutz, S. Meier, Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) using two Coating Formulations, 2012.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Kamke, Dominic</span> ; <span style=\"font-variant:small-caps;\">Bennyassine, A.</span> ; <span style=\"font-variant:small-caps;\">Kutz, Gerd</span> ; <span style=\"font-variant:small-caps;\">Meier, S.</span>: <i>Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) using two Coating Formulations</i>, 2012","van":"Kamke D, Bennyassine A, Kutz G, Meier S. Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) using two Coating Formulations. 2012.","mla":"Kamke, Dominic, et al. <i>Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) Using Two Coating Formulations</i>. 2012.","apa":"Kamke, D., Bennyassine, A., Kutz, G., &#38; Meier, S. (2012). <i>Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) using two Coating Formulations</i>. 8th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, Istanbul (Türkei).","short":"D. Kamke, A. Bennyassine, G. Kutz, S. Meier, Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) Using Two Coating Formulations, 2012.","ieee":"D. Kamke, A. Bennyassine, G. Kutz, and S. Meier, <i>Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) using two Coating Formulations</i>. 2012.","bjps":"<b>Kamke D <i>et al.</i></b> (2012) <i>Investigation of Film Coating Processes in an Innojet Air Coater (IAC 025) Using Two Coating Formulations</i>. ."},"status":"public","date_created":"2024-11-19T10:41:59Z"},{"_id":"12044","user_id":"83781","type":"conference_poster","conference":{"location":"Istanbul (Türkei)","start_date":"2012-03-19","end_date":"2012-03-22","name":"8th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology"},"date_updated":"2024-11-21T14:17:41Z","status":"public","citation":{"chicago":"Kellner, Theresa, Dominic Kamke, Gerd Kutz, and S. Dietrich . <i>Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control</i>, 2012.","chicago-de":"Kellner, Theresa, Dominic Kamke, Gerd Kutz und S. Dietrich . 2012. <i>Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control</i>.","ufg":"<b>Kellner, Theresa u. a.</b>: Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control, o. O. 2012.","ama":"Kellner T, Kamke D, Kutz G, Dietrich  S. <i>Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control</i>.; 2012.","short":"T. Kellner, D. Kamke, G. Kutz, S. Dietrich , Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control, 2012.","apa":"Kellner, T., Kamke, D., Kutz, G., &#38; Dietrich , S. (2012). <i>Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control</i>. 8th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, Istanbul (Türkei).","van":"Kellner T, Kamke D, Kutz G, Dietrich  S. Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control. 2012.","mla":"Kellner, Theresa, et al. <i>Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control</i>. 2012.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Kellner, Theresa</span> ; <span style=\"font-variant:small-caps;\">Kamke, Dominic</span> ; <span style=\"font-variant:small-caps;\">Kutz, Gerd</span> ; <span style=\"font-variant:small-caps;\">Dietrich , S.</span>: <i>Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control</i>, 2012","havard":"T. Kellner, D. Kamke, G. Kutz, S. Dietrich , Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control, 2012.","bjps":"<b>Kellner T <i>et al.</i></b> (2012) <i>Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control</i>. .","ieee":"T. Kellner, D. Kamke, G. Kutz, and S. Dietrich , <i>Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control</i>. 2012."},"date_created":"2024-11-21T14:17:21Z","title":"Continuous In-Line Particle Sizing during High-Shear Granulation for Process Control","author":[{"last_name":"Kellner","id":"85521","first_name":"Theresa","full_name":"Kellner, Theresa"},{"last_name":"Kamke","id":"82841","first_name":"Dominic","full_name":"Kamke, Dominic"},{"id":"12015","first_name":"Gerd","last_name":"Kutz","full_name":"Kutz, Gerd"},{"full_name":"Dietrich , S.","first_name":"S.","last_name":"Dietrich "}],"year":"2012","publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"DEP4022"}]},{"user_id":"83781","_id":"12045","type":"conference_speech","date_updated":"2024-11-21T14:24:20Z","conference":{"start_date":"2012-11-11","end_date":"2012-11-11","name":"Zentrum \"Neue Technologie\" des Deutschen Museums","location":"München"},"citation":{"chicago-de":"Kutz, Gerd. 2012. <i>Cosmetic formulations workshop</i>.","chicago":"Kutz, Gerd. <i>Cosmetic Formulations Workshop</i>, 2012.","ama":"Kutz G. <i>Cosmetic Formulations Workshop</i>.; 2012.","ufg":"<b>Kutz, Gerd</b>: Cosmetic formulations workshop, o. O. 2012.","havard":"G. Kutz, Cosmetic formulations workshop, 2012.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Kutz, Gerd</span>: <i>Cosmetic formulations workshop</i>, 2012","apa":"Kutz, G. (2012). <i>Cosmetic formulations workshop</i>. Zentrum “Neue Technologie” des Deutschen Museums, München.","mla":"Kutz, Gerd. <i>Cosmetic Formulations Workshop</i>. 2012.","van":"Kutz G. Cosmetic formulations workshop. 2012.","short":"G. Kutz, Cosmetic Formulations Workshop, 2012.","ieee":"G. Kutz, <i>Cosmetic formulations workshop</i>. 2012.","bjps":"<b>Kutz G</b> (2012) <i>Cosmetic Formulations Workshop</i>. ."},"status":"public","date_created":"2024-11-21T14:24:11Z","author":[{"full_name":"Kutz, Gerd","last_name":"Kutz","id":"12015","first_name":"Gerd"}],"title":"Cosmetic formulations workshop","language":[{"iso":"eng"}],"department":[{"_id":"DEP4022"}],"publication_status":"published","year":"2012"},{"publication_identifier":{"issn":["0024-3590","1939-5590"]},"_id":"12250","publication_status":"published","main_file_link":[{"open_access":"1","url":"https://doi.org/10.4319/lo.2012.57.2.0541"}],"page":"541-553","oa":"1","citation":{"ama":"Shatwell T, Nicklisch A, Köhler J. Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations. <i>Limnology and Oceanography</i>. 2012;57(2):541-553. doi:<a href=\"https://doi.org/10.4319/lo.2012.57.2.0541\">10.4319/lo.2012.57.2.0541</a>","ufg":"<b>Shatwell, Tom/Nicklisch, Andreas/Köhler, Jan</b>: Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations, in: <i>Limnology and Oceanography</i> 57 (2012), H. 2,  S. 541–553.","chicago":"Shatwell, Tom, Andreas Nicklisch, and Jan Köhler. “Temperature and Photoperiod Effects on Phytoplankton Growing under Simulated Mixed Layer Light Fluctuations.” <i>Limnology and Oceanography</i> 57, no. 2 (2012): 541–53. <a href=\"https://doi.org/10.4319/lo.2012.57.2.0541\">https://doi.org/10.4319/lo.2012.57.2.0541</a>.","chicago-de":"Shatwell, Tom, Andreas Nicklisch und Jan Köhler. 2012. Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations. <i>Limnology and Oceanography</i> 57, Nr. 2: 541–553. doi:<a href=\"https://doi.org/10.4319/lo.2012.57.2.0541\">10.4319/lo.2012.57.2.0541</a>, .","ieee":"T. Shatwell, A. Nicklisch, and J. Köhler, “Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations,” <i>Limnology and Oceanography</i>, vol. 57, no. 2, pp. 541–553, 2012, doi: <a href=\"https://doi.org/10.4319/lo.2012.57.2.0541\">10.4319/lo.2012.57.2.0541</a>.","bjps":"<b>Shatwell T, Nicklisch A and Köhler J</b> (2012) Temperature and Photoperiod Effects on Phytoplankton Growing under Simulated Mixed Layer Light Fluctuations. <i>Limnology and Oceanography</i> <b>57</b>, 541–553.","van":"Shatwell T, Nicklisch A, Köhler J. Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations. Limnology and Oceanography. 2012;57(2):541–53.","apa":"Shatwell, T., Nicklisch, A., &#38; Köhler, J. (2012). Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations. <i>Limnology and Oceanography</i>, <i>57</i>(2), 541–553. <a href=\"https://doi.org/10.4319/lo.2012.57.2.0541\">https://doi.org/10.4319/lo.2012.57.2.0541</a>","mla":"Shatwell, Tom, et al. “Temperature and Photoperiod Effects on Phytoplankton Growing under Simulated Mixed Layer Light Fluctuations.” <i>Limnology and Oceanography</i>, vol. 57, no. 2, 2012, pp. 541–53, <a href=\"https://doi.org/10.4319/lo.2012.57.2.0541\">https://doi.org/10.4319/lo.2012.57.2.0541</a>.","short":"T. Shatwell, A. Nicklisch, J. Köhler, Limnology and Oceanography 57 (2012) 541–553.","havard":"T. Shatwell, A. Nicklisch, J. Köhler, Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations, Limnology and Oceanography. 57 (2012) 541–553.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Shatwell, Tom</span> ; <span style=\"font-variant:small-caps;\">Nicklisch, Andreas</span> ; <span style=\"font-variant:small-caps;\">Köhler, Jan</span>: Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations. In: <i>Limnology and Oceanography</i> Bd. 57, Wiley (2012), Nr. 2, S. 541–553"},"status":"public","intvolume":"        57","date_updated":"2024-12-09T08:42:06Z","extern":"1","quality_controlled":"1","type":"scientific_journal_article","volume":57,"doi":"10.4319/lo.2012.57.2.0541","user_id":"83781","department":[{"_id":"DEP8022"}],"language":[{"iso":"eng"}],"publication":"Limnology and Oceanography","year":"2012","author":[{"orcid":"0000-0002-4520-7916","last_name":"Shatwell","first_name":"Tom","id":"86424","full_name":"Shatwell, Tom"},{"last_name":"Nicklisch","first_name":"Andreas","full_name":"Nicklisch, Andreas"},{"last_name":"Köhler","first_name":"Jan","full_name":"Köhler, Jan"}],"title":"Temperature and photoperiod effects on phytoplankton growing under simulated mixed layer light fluctuations","date_created":"2024-12-08T20:45:17Z","issue":"2","abstract":[{"text":"We measured specific growth rates of Stephanodiscus minutulus, Nitzschia acicularis (diatoms), and Limnothrix redekei (cyanobacterium) under fluctuating and constant light in semi-continuous culture at 10°C, 15°C, and 20°C and under photoperiods of 6 h d−1 and 12 h d−1. Fluctuating light regimes simulated regular vertical mixing in lakes with a ratio of euphotic to mixed depth (zeu : zmix) of 1 and 0.5 on a cloudless day. Light fluctuations at zeu : zmix = 1 decreased the growth rates of S. minutulus, N. acicularis, and L. redekei by 18%, 33%, and 29%, respectively, compared to constant light at the same daily light supply. Temperature had no effect on this decrease. Halving zeu : zmix (simulating deep mixing) had the same effect on growth as halving the photoperiod, demonstrating that these factors are cumulative. We introduce a simple empirical factor to adjust growth rates measured under constant light to account for fluctuating light. This factor is independent of temperature and photoperiod, applies over a range of zeu : zmix, and accurately describes present and published growth rates of several species. We show how to account for temporal variability of the light supply at different temperatures and photoperiods when predicting growth rates of phytoplankton.","lang":"eng"}],"publisher":"Wiley"},{"language":[{"iso":"eng"}],"department":[{"_id":"DEP4010"}],"year":"2012","publication":"BMC Infectious Diseases","author":[{"orcid":"https://orcid.org/0009-0008-7644-0826","full_name":"Dabisch-Ruthe, Mareike","last_name":"Dabisch-Ruthe","id":"66516","first_name":"Mareike"},{"first_name":"Tanja","last_name":"Vollmer","full_name":"Vollmer, Tanja"},{"last_name":"Adams","first_name":"Ortwin","full_name":"Adams, Ortwin"},{"last_name":"Knabbe","first_name":"Cornelius","full_name":"Knabbe, Cornelius"},{"first_name":"Jens","last_name":"Dreier","full_name":"Dreier, Jens"}],"title":"Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay","issue":"1","date_created":"2025-06-17T06:52:13Z","publisher":"Springer","abstract":[{"lang":"eng","text":"Background\r\nA broad spectrum of pathogens is causative for respiratory tract infections, but symptoms are mostly similar. Therefore, the identification of the causative viruses and bacteria is only feasible using multiplex PCR or several monoplex PCR tests in parallel.\r\nMethods\r\nThe analytical sensitivity of three multiplex PCR assays, RespiFinder-19, RespiFinder-SMART-22 and xTAG-Respiratory-Virus-Panel-Fast-Assay (RVP), were compared to monoplex real-time PCR with quantified standardized control material. All assays include the most common respiratory pathogens.\r\nResults\r\nTo compare the analytical sensitivity of the multiplex assays, samples were inoculated with 13 different quantified viruses in the range of 101 to 105 copies/ml. Concordant results were received for rhinovirus, whereas the RVP detected influenzavirus, RSV and hMPV more frequently in low concentrations. The RespiFinder-19 and the RespiFinder-SMART-22 showed a higher analytical sensitivity for adenoviruses and coronaviruses, whereas the RVP was incapable to detect adenovirus and coronavirus in concentrations of 104 copies/ml. The RespiFinder-19 and RespiFinder-SMART-22A did not detect influenzaviruses (104 copies/ml) and RSV (103 copies/ml). The detection of all 13 viruses in one sample was only achieved using monoplex PCR. To analyze possible competitive amplification reactions between the different viruses, samples were further inoculated with only 4 different viruses in one sample. Compared to the detection of 13 viruses in parallel, only a few differences were found.\r\nThe incidence of respiratory viruses was compared in tracheal secretion (TS) samples (n = 100) of mechanically ventilated patients in winter (n = 50) and summer (n = 50). In winter, respiratory viruses were detected in 32 TS samples (64%) by RespiFinder-19, whereas the detection rate with RVP was only 22%. The most frequent viruses were adenovirus (32%) and PIV-2 (20%). Multiple infections were detected in 16 TS samples (32%) by RespiFinder-19. Fewer infections were found in summer (RespiFinder-19: 20%; RVP: 6%). All positive results were verified using monoplex PCR.\r\nConclusions\r\nMultiplex PCR tests have a broad spectrum of pathogens to test at a time. Analysis of multiple inoculated samples revealed a different focus of the detected virus types by the three assays. Analysis of clinical samples showed a high concordance of detected viruses by the RespiFinder-19 compared to monoplex tests."}],"article_number":"163","intvolume":"        12","date_updated":"2025-06-17T13:49:03Z","quality_controlled":"1","type":"scientific_journal_article","volume":12,"doi":"10.1186/1471-2334-12-163","user_id":"83781","publication_status":"published","citation":{"ufg":"<b>Dabisch-Ruthe, Mareike u. a.</b>: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, in: <i>BMC Infectious Diseases</i> 12 (2012), H. 1.","ieee":"M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, and J. Dreier, “Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay,” <i>BMC Infectious Diseases</i>, vol. 12, no. 1, Art. no. 163, 2012, doi: <a href=\"https://doi.org/10.1186/1471-2334-12-163\">10.1186/1471-2334-12-163</a>.","ama":"Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. <i>BMC Infectious Diseases</i>. 2012;12(1). doi:<a href=\"https://doi.org/10.1186/1471-2334-12-163\">10.1186/1471-2334-12-163</a>","bjps":"<b>Dabisch-Ruthe M <i>et al.</i></b> (2012) Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay. <i>BMC Infectious Diseases</i> <b>12</b>.","chicago-de":"Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe und Jens Dreier. 2012. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. <i>BMC Infectious Diseases</i> 12, Nr. 1. doi:<a href=\"https://doi.org/10.1186/1471-2334-12-163\">10.1186/1471-2334-12-163</a>, .","havard":"M. Dabisch-Ruthe, T. Vollmer, O. Adams, C. Knabbe, J. Dreier, Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay, BMC Infectious Diseases. 12 (2012).","din1505-2-1":"<span style=\"font-variant:small-caps;\">Dabisch-Ruthe, Mareike</span> ; <span style=\"font-variant:small-caps;\">Vollmer, Tanja</span> ; <span style=\"font-variant:small-caps;\">Adams, Ortwin</span> ; <span style=\"font-variant:small-caps;\">Knabbe, Cornelius</span> ; <span style=\"font-variant:small-caps;\">Dreier, Jens</span>: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. In: <i>BMC Infectious Diseases</i> Bd. 12. Berlin ; Heidelberg, Springer (2012), Nr. 1","chicago":"Dabisch-Ruthe, Mareike, Tanja Vollmer, Ortwin Adams, Cornelius Knabbe, and Jens Dreier. “Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” <i>BMC Infectious Diseases</i> 12, no. 1 (2012). <a href=\"https://doi.org/10.1186/1471-2334-12-163\">https://doi.org/10.1186/1471-2334-12-163</a>.","mla":"Dabisch-Ruthe, Mareike, et al. “Comparison of Three Multiplex PCR Assays for the Detection of Respiratory Viral Infections: Evaluation of XTAG Respiratory Virus Panel Fast Assay, RespiFinder 19 Assay and RespiFinder SMART 22 Assay.” <i>BMC Infectious Diseases</i>, vol. 12, no. 1, 163, 2012, <a href=\"https://doi.org/10.1186/1471-2334-12-163\">https://doi.org/10.1186/1471-2334-12-163</a>.","apa":"Dabisch-Ruthe, M., Vollmer, T., Adams, O., Knabbe, C., &#38; Dreier, J. (2012). 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