@inbook{10077, author = {{Felmeden, Jörg}}, booktitle = {{Controlling in der kommunalen Umweltwirtschaft : Anforderungen- Aufgabenfelder - Instrumente}}, editor = {{Wöbbeking, Karl H.}}, isbn = {{978-3-503-15681-8}}, keywords = {{Planung, Recht, Umweltschutz}}, pages = {{191--253 }}, publisher = {{E. Schmidt }}, title = {{{Nachhaltige Zukunftsstrategien für die Wasserwirtschaft}}}, year = {{2014}}, } @misc{10083, author = {{Felmeden, Jörg}}, issn = {{1614-8193}}, keywords = {{Bewässerung, Iran, Landwirtschaft}}, pages = {{50}}, publisher = {{ISOE - Institut für sozial-ökologische Forschung }}, title = {{{Agriculture in the Zayandeh Rud Catchment. With support of Engelbert Schramm, Elnaz Sattary, Arash Davoudi}}}, volume = {{40}}, year = {{2014}}, } @inbook{10094, author = {{Felmeden, Jörg and Winker, Martina}}, booktitle = {{Intelligente und multifunktionale Infrastruktursysteme für eine zukunftsfähige Wasserversorgung und Abwasserentsorgung (INIS)}}, pages = {{14--15}}, publisher = {{Deutsches Institut für Urbanistik gGmbH (Difu)}}, title = {{{netWORKS 3 - Intelligente wasserwirtschaftliche Systemlösungen in Frankfurt am Main und Hamburg}}}, year = {{2014}}, } @misc{10166, author = {{Dünnermann, Jens and Fuchs, Stefan and Schmidt, Hans-Peter and Witte, Stefan}}, location = {{Paderborn, Germany}}, publisher = {{IEEE}}, title = {{{Demo: Real time Ethernet with unshielded twisted single pair cabling}}}, year = {{2014}}, } @misc{4884, author = {{Gausemeier, Jürgen and Dumitrescu, Roman and Jasperneite, Jürgen and Kühn, Arno and Trsek, Henning}}, publisher = {{Imagebroschüre des Spitzenclusters zum Thema Industrie 4.0}}, title = {{{Auf dem Weg zu Industrie 4.0: Lösungen aus dem Spitzencluster it´s OWL}}}, year = {{2014}}, } @misc{4928, author = {{Vetter, Andreas}}, publisher = {{Galerie 'Plattenpalast'}}, title = {{{Das Konkrete und die Architektur}}}, year = {{2014}}, } @article{5038, author = {{Büdicker, K. and Deister, U. and Fettig, Joachim and Glinka, U. and Götz, M. and Rudolph, B.}}, journal = {{VDI UmweltMagazin }}, pages = {{3}}, title = {{{10 Jahre Treffen der Umwelttechnikstudiengänge}}}, volume = {{44}}, year = {{2014}}, } @inbook{5061, author = {{Fettig, Joachim and Pick, Volker}}, booktitle = {{Contributions by EWATEC-COAST to 4th International Conference for Environment and National Resources, ICENR 2014}}, editor = {{Meon, G.}}, pages = {{194--203}}, publisher = {{Cuvillier}}, title = {{{Treatment of Tannery Wastewater by Enhanced Biological Processes – Preliminary Results}}}, year = {{2014}}, } @article{5191, author = {{Iseke, Anja and Kocks, Birgit and Schneider, Martin R. and Schulze-Bentrop, Conrad}}, issn = {{0033-6807}}, journal = {{R&D Management}}, pages = {{23--40}}, title = {{{Cross-cutting organizational and demographic divides and the performance of research and development teams: two wrongs can make a right}}}, doi = {{10.1111/radm.12049}}, volume = {{ Vol. 45 (1)}}, year = {{2014}}, } @inproceedings{5220, abstract = {{Façade plays an important role in modern movement architecture and a critical role in the conception of energy and climate-optimized buildings: it is the building's skin and functions as an interface between interior and exterior space. It provides thermal and sound insulation and ventilation, and controls and guides the entrance of daylight into the building. Tropical climates have unique challenges to reduce solar heat gain through building envelope to make buildings energy efficient. India has a rich tradition of different forms of architecture and design principles - most of them are perfectly adapted to the climatic requirements and constructed with local building materials and technology. In the twentieth century, Modern Movement Architecture shows a global trend towards transparent, light-flled buildings with great expanses of windows and façades with advanced building technology. However, the twentieth century modern movement architecture in India evolved in response to the climate and culture. In India, remarkably diverse constellations of façade design can be traced in modern architecture often taking into consideration traditional Indian principles combining them with new materials and constructions. Most of them marked by overhanging balconies and horizontal brise-soleils and as an example of a human scaled urban infrastructure offering private and public green spaces in transition. After independence in 1947 more public, administrative and governmental buildings followed in the tradition of the modern movement and with emblematic character. This paper presents a review of the influences of modern movement architecture on today's facade design in tropical climate of India. The purpose of this review is to reappraise works of the masters of the modern movement, in the context of today's energy and environmental value system; a value system which justifies a greater capital cost in money terms with a view to minimize recurrent costs in energy terms and environmental degradation.}}, author = {{Kabre, Chitra and Pottgiesser, Uta}}, booktitle = {{Proceedings of the 13th International DOCOMOMO Conference: Expansion and Conflict}}, editor = {{Tostões, Ana}}, isbn = {{979-11-953625-0-9}}, location = {{Seoul, Korea}}, title = {{{A review of influences of modern movement architecture on today's façade design in tropical climate of India}}}, year = {{2014}}, } @inproceedings{5221, author = {{Martau, Betina Tschiedel and da Silva, F. Duro and Pottgiesser, Uta}}, booktitle = {{Proceedings of 5th seminar DOCOMOMO Norte/Nordeste}}, location = {{Fortaleza: Brazil}}, title = {{{Estratégias de intervenções para requalificação e valorização do patrimônio arquitetônico moderno : estudo de caso no edificio da smov em Porto Alegre}}}, year = {{2014}}, } @inproceedings{5222, author = {{Doleschal, Reinhard and Pottgiesser, Uta and Akhilesh, K. B. and Kabre, Chitrarekha and König, Katharina}}, booktitle = {{Sustainable habitat for developing societies : choosing the way forward : 30th International PLEA Conference, December 16-18, 2014 : book of abstracts}}, editor = {{Rawal, Rajan}}, isbn = {{978-93-83184-02-6 }}, location = {{Ahmedabad, India }}, publisher = {{CEPT University Press}}, title = {{{Integrating user awareness and behavior into building and product design for India : survey in eight giant cities in India}}}, year = {{2014}}, } @proceedings{5223, editor = {{Tomlow, Jos and Dill, Alex and Pottgiesser, Uta}}, isbn = {{ 978-3-929744-89-7 }}, location = {{Karlsruhe}}, number = {{13}}, pages = {{ 88 Seiten }}, publisher = {{Graphische Werkstätten Zittau }}, title = {{{Perceived technologies in the modern movement 1918-1975 : proceedings of the 13th International DOCOMOMO Technology Seminar, january 25/26 2013, Karlsruhe . the seminar figures also as the 10. Karlsruher Tagung "Das architektonische Erbe" / International Working Party for Documentation and Conservation of Buildings, Sites and Neighbourhoods of the Modern Movement }}}, year = {{2014}}, } @inproceedings{5226, author = {{Tomolow, Jos and Dill, Alex and Pottgiesser, Uta}}, isbn = {{ 978-3-929744-89-7 }}, number = {{ 13 }}, pages = {{ 88 Seiten }}, publisher = {{ Graphische Werkstätten Zittau }}, title = {{{ Perceived technologies in the modern movement 1918-1975. proceedings of the 13th International DOCOMOMO Technology Seminar, january 25/26 2013, Karlsruhe . the seminar figures also as the 10. Karlsruher Tagung "Das architektonische Erbe" }}}, year = {{2014}}, } @inproceedings{5442, author = {{Broeker, Timo and Hoffarth, M. and Schneider, Jan and Heikrodt, Klaus}}, booktitle = {{12. Internationaler Fachkongress für Biokraftstoffe "Fuels of the Future" (Vortrag und Tagungsbandbeitrag), Berlin, 20.01.2014}}, location = {{Berlin}}, title = {{{BioCo2nvert - Synergies of P2G and alcoholic CO2 for the efficient production of methan and bioethanol}}}, year = {{2014}}, } @inproceedings{5448, author = {{Lüttke Föller, A. and Schwarzer, Knut and Wilhelm, Patrick and Müller, Ulrich}}, booktitle = {{24. Bernburger Winterseminar Arznei- und Gewürzpflanzen : 18.02. - 19.02.2014 ; Tagungsbroschüre }}, editor = {{Hoppe, Bernd}}, location = {{Bernburg}}, publisher = {{Verein für Arznei- und Gewürzpflanzen Saluplanta }}, title = {{{Einfluss der Evakuierungsgeschwindigkeit auf die Majoranölverluste bei der Sattdampfbehandlung}}}, year = {{2014}}, } @inproceedings{5449, author = {{Dammann_, Anna and Schwarzer, Knut and Müller, Ulrich and Schneider, Jan}}, location = {{Freising}}, title = {{{Simulation zur Flaschen-Vollpasteurisation - Simulation zur Flaschen-Vollpasteurisation}}}, year = {{2014}}, } @inproceedings{5450, author = {{Pliske, Roland and Müller, Ulrich and Kohlus, R:}}, location = {{Freising}}, title = {{{Wärmeübergang bei der dynamischen Gefriertrocknung in Feststoffmischern}}}, year = {{2014}}, } @inproceedings{5451, author = {{Hüttemann, N. and Schwarzer, Knut and Wilhelm, Patrick and Müller, Ulrich}}, booktitle = {{ProcessNet-Fachgruppe Lebensmittelverfahrenstechnik, Freising, 26.-28.02.2014}}, location = {{Freising}}, title = {{{Untersuchung zur Zerstörung der ätherischen Ölzellen bei der Sattdampfbehandlung von Planzenmaterial}}}, year = {{2014}}, } @inproceedings{5452, author = {{Lüttke Föller, A. and Schwarzer, Knut and Wilhelm, Patrick and Müller, Ulrich}}, location = {{Freising}}, title = {{{Einfluss der Evakuierungsgeschwindigkeit auf die Majoranölverluste bei der Sattdampfbehandlung}}}, year = {{2014}}, } @inproceedings{5453, author = {{Cornu, Jesahel and Lüttke Föller, A. and Wilhelm, Patrick and Müller, Ulrich}}, location = {{Frankfurt-Rodgau}}, title = {{{Wirksame Kondensatfilmdicke bei der mechanischen Sattdampfentkeimung von pflanzlichen Materialien}}}, year = {{2014}}, } @inproceedings{5454, author = {{Stiebing, Achim}}, booktitle = {{ Fortbildungsseminar des Bundesverbandes der Deutschen Fleischwarenindustrie (BVDF), Rheinhardsbrunn, 15./16.03.2013 und 13./14.03.2014}}, location = {{Rheinhardsbrunn}}, title = {{{Technologien zur Herstellung von Kochschinken und Kochwurst. Neue Zutaten und Zusatzstoffe, Chancen, Grenzen und Technologien. Betriebliche QS-Konzepte für die Roh- und Brühwurstherstellung}}}, year = {{2014}}, } @inproceedings{5455, author = {{Broeker, Timo and Blöhse, D. and Bittner, J. and Ramke, Hans-Günter and Schneider, Jan}}, booktitle = {{10th European Bioethanol and Bioconversion Technology Meeting, Detmold, 08.04.2014}}, location = {{Detmold}}, title = {{{How to extend a distillery into a lignocellulose biorefinery – Material - & Energy Flow Management of Bioethanol, Biogas & HTC Biochar}}}, year = {{2014}}, } @inproceedings{5456, author = {{Stiebing, Achim}}, booktitle = {{Rotary Lemgo, 14.04.2014}}, location = {{Lemgo}}, title = {{{Fleisch und Fleischerzeugnisse im Kreuzfeuer der Kritik}}}, year = {{2014}}, } @inproceedings{5458, author = {{Schneider, Jan and Schwarzer, Knut and Dammann, Anna and Müller, Ulrich}}, booktitle = {{11th International Trends in Brewing, Ghent (Belgium), April 13th-17th 2014}}, location = {{Ghent (Belgium)}}, title = {{{Effect of residence time distribution in flash pasteurizers on the thermal death oEffect of the residence time distribution in flash pasteurizers on the thermal death of microorganism under consideration of the heating and cooling sectionsf microorganisms}}}, year = {{2014}}, } @article{5460, author = {{Zapp, Jürgen and Kothe, Achim and Münstermann, J.}}, issn = {{0344-5208 }}, journal = {{LABO – Fit for Lab}}, number = {{1-2}}, pages = {{12--14}}, title = {{{Welche Stoffe in harten Getränken?}}}, year = {{2014}}, } @article{5462, author = {{Damman, Anna and Schwarzer, Knut and Müller, Ulrich and Schneider, Jan}}, issn = {{1521-3811}}, journal = {{Lebensmittelchemie : Zeitschrift d. Lebensmittelchemischen Gesellschaft, Fachgruppe in der Gesellschaft Deutscher Chemiker}}, number = {{6}}, pages = {{151}}, publisher = {{Wiley-VCH}}, title = {{{Backverhalten und Sensorik eines Färberdistelblüteneytraktes am Beispiel Rührkuchen}}}, doi = {{10.1002/lemi.201490056}}, volume = {{68}}, year = {{2014}}, } @article{5477, author = {{Münstermann, J. and Kothe, Achim and Zapp, Jürgen}}, issn = {{1521-3811}}, journal = {{Lebensmittelchemie : Zeitschrift d. Lebensmittelchemischen Gesellschaft, Fachgruppe in der Gesellschaft Deutscher Chemiker}}, number = {{6}}, pages = {{152}}, publisher = {{Wiley-VCH}}, title = {{{Analytik von Farbstoffen aus den Blüten der Färberdistel Carthamus tinctorius}}}, doi = {{10.1002/lemi.201490056}}, volume = {{68}}, year = {{2014}}, } @inproceedings{5478, author = {{Broeker, Timo and Barten, G.}}, booktitle = {{EnergieAgentur.NRW 3, Wuppertal (2014)}}, location = {{Wuppertal}}, title = {{{Biokraftstoffe aus Rest- und Abfallstoffen? Pro & Contra; Innovation & Energie}}}, year = {{2014}}, } @article{5479, author = {{Woest, H. and Cöllü, I. and Dammann, Anna and Schwarzer, Knut and Schneider, Jan}}, issn = {{0179-2466}}, journal = {{Brauerei-Forum : Fachzeitschrift für Brauereien und Mälzereien, Getränkeindustrie und deren Partner }}, number = {{8}}, publisher = {{Westkreuz-Verl. }}, title = {{{Forschungsprojekt zur nachhaltigen und schonenden Getränkepasteurisation abgeschlossen}}}, year = {{2014}}, } @article{5480, author = {{Schneider, Jan}}, issn = {{0936-4129 }}, journal = {{Getränkeherstellung Deutschland : Informationen für die Wirtschaft }}, publisher = {{Kuhn}}, title = {{{Kundennähe – Wieviel Nähe darf es denn sein? }}}, year = {{2014}}, } @inproceedings{5481, author = {{Stiebing, Achim}}, booktitle = {{Museumsrunde Detmold, 01.06.2014}}, location = {{Detmold}}, title = {{{Ebermast - ... Ein neuer Weg?}}}, year = {{2014}}, } @misc{5482, abstract = {{The invention relates to a method for producing enriched peptide fractions from protein-containing raw materials, in which protein hydrolysates are separated using chromatography, according to the physiochemical properties thereof, by means of stationary phases with an aqueous solution as an elution agent. }}, author = {{Danneel, Hans-Jürgen}}, title = {{{Method For Producing Peptide Fractions And Use Thereof; Offenlegungsschrift}}}, year = {{2014}}, } @inproceedings{5483, author = {{Stiebing, Achim}}, booktitle = {{Zoetis Seminar, Potsdam, 26.06.2014}}, location = {{Potsdam}}, title = {{{Impfung gegen Ebergeruch - Verarbeitungseigenschaften}}}, year = {{2014}}, } @inproceedings{5484, author = {{Bernert, Till and Zapp, Jürgen}}, booktitle = {{41. Deutscher Lebensmittelchemikertag, Münster, 10.-12.09.2014}}, location = {{Münster}}, title = {{{Polyphenolgehalte in alten und neuen Apfelsorten in Bezug auf allergene Wirkungen}}}, year = {{2014}}, } @inproceedings{5485, author = {{Steffens, M. and Schneider, Jan}}, booktitle = {{GDL Kongress Lebensmitteltechnologie 2014, Frankfurt-Rodgau, 16.10-18.10.2014}}, location = {{Frankfurt-Rodgau}}, title = {{{Bindung von Eisen an Caseinphosphopeptide}}}, year = {{2014}}, } @inproceedings{5486, author = {{Pliske, Roland and Müller, Ulrich and Kohlus, R.}}, booktitle = {{GDL Kongress Lebensmitteltechnologie 2014, Frankfurt-Rodgau, 16.10-18.10.2014}}, location = {{Frankfurt-Rodgau}}, title = {{{Bestimmung von der Trocknungsgeschwindigkeit und des Wärmeübergangskoeffizienten bei der dynamischen Gefriertrocknung von Starterkulturen in einem Feststoffmischer}}}, year = {{2014}}, } @inproceedings{5487, author = {{Müller, Ulrich}}, booktitle = {{GDL Kongress Lebensmitteltechnologie 2014, Frankfurt-Rodgau, 16.10-18.10.2014}}, location = {{Frankfurt-Rodgau}}, title = {{{ Entkeimungsverfahren und biologische Stabilisierung von Lebensmitteln - Behandlungsverfahren für fluide und feste Substanzen aus Sicht eines Lebensmittelverfahrenstechnikers}}}, year = {{2014}}, } @inproceedings{5488, author = {{Stiebing, Achim}}, booktitle = {{37. Lemgoer Arbeitstagung Fleisch und Feinkost, 03.11.2014}}, location = {{Lemgo}}, title = {{{Gefrierlagerstabilität von Fleischerzeugnissen - Anforderungen an fleischhaltige TK-Produkte}}}, year = {{2014}}, } @inproceedings{5489, author = {{Stiebing, Achim}}, booktitle = {{Fortbildungsseminar des Bundesverbandes der Deutschen Fleischwarenindustrie (BVDF), Rheinhardsbrunn, 16.-17.11.2013 und 15.-16.11.2014}}, location = {{Rheinhardsbrunn}}, title = {{{Technologie der Rohschinkenherstellung - Technologie der Rohwurstherstellung}}}, year = {{2014}}, } @inproceedings{5490, author = {{Broeker, Timo and Blöhse, D. and Bittner, J. and Ramke, Hans-Günter and Schneider, Jan}}, booktitle = {{Biokraftstofftagung NRW, Haus Düsse, Landwirtschaftskammer NRW, Bad Sassendorf, 27.11.2014}}, location = {{Bad Sassendorf}}, title = {{{Erweiterung einer Brennerei zu einer Lignocellulose-Bioraffinerie - Stoff- und Energiestrom Management für Bioethanol, Biogas und HTC-Biokohle}}}, year = {{2014}}, } @misc{12145, author = {{Hüttner, C. and Kutz, Gerd and Dietrich, S. }}, location = {{Graz (Österreich)}}, title = {{{PAT in High-Shear Granulation Processes using In-line Particle Size Measurements}}}, year = {{2014}}, } @misc{12146, author = {{Hüttner , C. and Türken, B. and Kutz, Gerd and Dietrich, Stefan}}, location = {{Lissabon (Portugal)}}, title = {{{Real time process control during wet granulation in a high-shear mixer using Spatial Filter Velocimetry - Influence of different formulations }}}, year = {{2014}}, } @misc{12157, author = {{Lewandowski, E. and Kamke, Dominic and Kutz, Gerd and Dietrich, S.}}, location = {{Lissabon (Portugal)}}, title = {{{Real time particle size measurement during high-shear melt granulation}}}, year = {{2014}}, } @misc{12246, abstract = {{In lakes, trophic change and climate change shift the relationship between nutrients and physical factors, like temperature and photoperiod, and interactions between these factors should affect the growth of phytoplankton species differently. We therefore determined the relationship between P-limited specific growth rates and P-quota (biovolume basis) of Stephanodiscus minutulus and Nitzschia acicularis (diatoms) at or near light saturation in axenic, semi-continuous culture at 10, 15 and 20 °C and at 6, 9 and 12 h d−1 photoperiod. Photoperiod treatments were performed at constant daily light exposure to allow comparison. Under these conditions, we also performed competition experiments and estimated relative P-uptake rates of the species. Temperature strongly affected P-limited growth rates and relative P uptake rates, whereas photoperiod only affected maximum growth rates. S. minutulus used internal P more efficiently than N. acicularis. N. acicularis was the superior competitor for P due to a higher relative uptake rate and its superiority increased with increasing temperature and photoperiod. S. minutulus conformed to the Droop relationship but N. acicularis did not. A model with a temperature-dependent normalised half-saturation coefficient adequately described the factor interactions of both species. The temperature dependence of the quota model reflected each species’ specific adaptation to its ecological niche. The results demonstrate that increases in temperature or photoperiod can partially compensate for a decrease in P-quota under moderately limiting conditions, like during spring in temperate lakes. Thus warming may counteract de-eutrophication to some degree and a relative shift in growth factors can influence the phytoplankton species composition.}}, author = {{Shatwell, Tom and Köhler, Jan and Nicklisch, Andreas}}, booktitle = {{PLoS ONE}}, issn = {{1932-6203}}, number = {{7}}, publisher = {{Public Library of Science (PLoS)}}, title = {{{Temperature and Photoperiod Interactions with Phosphorus-Limited Growth and Competition of Two Diatoms}}}, doi = {{10.1371/journal.pone.0102367}}, volume = {{9}}, year = {{2014}}, } @misc{12979, abstract = {{Physiological consequences of adaptation to and continued feeding of a high-energetic diet were studied in eight non-pregnant, non-lactating dairy Holstein cows over a period of 16 weeks. The first six weeks served as an adaptation period from the low energetic straw-based diet (3.8 MJ NEL/kg DM) to the high-energetic ration (7.5 MJ NEL/kg DM). Intake of dry matter (DM) increased with dietary energy concentration from 9 to 20 kg/d up to week 9 to 12 and decreased thereafter. The initial live weight (LW) of 550 ± 60 kg was increased linearly and corresponded to an average daily LW gain of 2.3 ± 0.3 kg. Energy balance increased approximately nine-fold to a maximum of 114 MJ NEL/d in week 10. Ruminal fermentation pattern was completely changed from an acetate dominating profile to a propionate based one, which was paralleled by a marked increase in the rumen fluid endotoxin concentration. Unlike blood glucose concentration, which increased continuously, that of cholesterol and triglycerides started to increase after an initial stagnation. In conclusion, both ruminal adaptation to a high-energetic diet and the continued feeding of such a diet induced digestive and metabolic adaptations in non-pregnant, non-lactating cows characterised by a progressing positive energy balance.}}, author = {{Dänicke, Sven and Meyer, Ulrich and Winkler, Janine and Schulz, Kirsten and Ulrich, Sebastian and Frahm, Jana and Kersten, Susanne and Rehage, Jürgen and Breves, Gerhard and Häußler, Susanne and Sauerwein, Helga and Locher, Lena}}, booktitle = {{Archives of Animal Nutrition}}, issn = {{1477-2817}}, keywords = {{blood chemistry, dairy cows, endotoxins, energy balance, energy content, rumen fermentation}}, number = {{6}}, pages = {{460--477}}, publisher = {{Taylor & Francis}}, title = {{{Description of a bovine model for studying digestive and metabolic effects of a positive energy balance not biased by lactation or gravidity}}}, doi = {{10.1080/1745039x.2014.973243}}, volume = {{68}}, year = {{2014}}, } @misc{12984, abstract = {{Background Pseudoxanthoma elasticum (PXE) is a rare hereditary disorder characterized by late onset and progressive calcification of elastic fibers in skin, eyes and the cardiovascular system, exemplifying a model for conditions characterized by soft tissue calcification. Objective The aim of our study was to characterize cellular inorganic pyrophosphate (PPi) homeostasis in PXE. Methods Gene expression of PPi metabolizing enzymes was determined by quantitative real-time PCR after incubation up to 21 days with or without addition of Na2HPO4. Extracellular and cytosolic PPi concentrations were measured by enzyme-linked bioluminescence assay. ALP and ENPP1 activity was determined spectrophotometrically. We further established a human cell culture model suitable for investigating PXE and related disorders without addition of artificial calcification triggers. Results Independently of the experimental conditions, PXE fibroblasts revealed a higher degree of matrix calcification. We observed that matrix calcification was associated with altered gene expression of PPi metabolizing enzymes in PXE fibroblasts. In this context, PXE fibroblasts exhibited significantly higher expression of ALP and OPN and reduced mRNA expression and activity of ENPP1. Here, for the first time cytosolic and extracellular PPi levels were shown to be strongly reduced in PXE fibroblasts. We further showed that PPi concentration in bovine and human sera additives had a strong impact on matrix calcification. In a last experimental line, we demonstrated that addition of PPi analogs reduced matrix calcification of PXE fibroblasts most likely by reducing ALP and OPN mRNA expression, restoring ENPP1 activity and subsequently elevating PPi concentrations. Conclusion The results of our study along with recent findings point to the essential role of PPi as the central regulatory metabolites preventing matrix calcification in PXE. But what remains to be determined is the underlying molecular mechanism leading to depletion of PPi in PXE. We further suggest that supplementation of PPi analogs might counteract pathological calcification in PXE and related disorders.}}, author = {{Dabisch-Ruthe, Mareike and Kuzaj, Patricia and Götting, Christian and Knabbe, Cornelius and Hendig, Doris}}, booktitle = {{Journal of Dermatological Science}}, issn = {{1873-569X}}, keywords = {{Pseudoxanthoma elasticum, Calcification, Pyrophosphate, Bisphosphonate, ABCC6, Tissue nonspecific alkaline phosphate, Ectonucleotide pyrophosphatase 1}}, number = {{2}}, pages = {{109--120}}, publisher = {{Elsevier }}, title = {{{Pyrophosphates as a major inhibitor of matrix calcification in Pseudoxanthoma elasticum}}}, doi = {{10.1016/j.jdermsci.2014.04.015}}, volume = {{75}}, year = {{2014}}, } @misc{12985, abstract = {{Objectives Pseudoxanthoma elasticum (PXE) is a rare hereditary disorder characterized by progressive calcification and fragmentation of elastic fibers. Because of the great clinical variability between PXE patients the involvement of modifier genes was recently suggested. Therefore, we investigated the association of single nucleotide variants (SNVs) in selected candidate genes known to regulate cellular pyrophosphate metabolism. Design and methods We used RLFP analyses to evaluate the distribution of SNVs in alkaline phosphatase (ALP), ectonucleotide pyrophosphatase 1 (ENPP1) and ankylosis (ANKH) in DNA samples from 190 German PXE patients and 190 age- and sex-matched healthy controls. Statistical analyses were performed using Fisher exact test and Bonferroni correction. Results The screening revealed three different SNVs in three genes, which were associated with PXE. The SNV c.1190-65C > A (rs1780329, minor allele frequency (MAF) patients: 0.17; controls: 0.11; P = 0.04) in the ALP gene was significantly more frequent in PXE patients. Furthermore, PXE was highly associated with ANKH p.A98A genotype TT (P = 0.0012), although the MAF was not different between patients and controls. After correction for multiple testing according to the Bonferroni method, one SNV in the ENPP1 gene (c.313 + 9G > T, rs7773477) remained significantly associated with PXE with significantly higher MAF values in the patient cohort (MAF: 0.04 vs. 0.00; P = 0.0024) and a high association with PXE susceptibility (OR 27.96). Conclusion Polymorphisms in ALP, ENPP1 and ANKH are important genetic risk factors contributing to PXE.}}, author = {{Dabisch-Ruthe, Mareike and Brock, Alexander and Kuzaj, Patricia and Charbel Issa, Peter and Szliska, Christiane and Knabbe, Cornelius and Hendig, Doris}}, booktitle = {{Clinical Biochemistry}}, issn = {{1873-2933}}, keywords = {{Pseudoxanthoma elasticum, Calcification, Tissue nonspecific alkaline phosphatase, Ectonucleotide pyrophosphatase 1, Ankylosis}}, number = {{15}}, pages = {{60--67}}, publisher = {{Elsevier}}, title = {{{Variants in genes encoding pyrophosphate metabolizing enzymes are associated with Pseudoxanthoma elasticum}}}, doi = {{10.1016/j.clinbiochem.2014.07.003}}, volume = {{47}}, year = {{2014}}, } @misc{12986, abstract = {{Background Dysregulations in cholesterol and lipid metabolism have been linked to human diseases like hypercholesterolemia, atherosclerosis or the metabolic syndrome. Many ABC transporters are involved in trafficking of metabolites derived from these pathways. Pseudoxanthoma elasticum (PXE), an autosomal-recessive disease caused by ABCC6 mutations, is characterized by atherogenesis and soft tissue calcification. Methods In this study we investigated the regulation of cholesterol biosynthesis in human dermal fibroblasts from PXE patients and healthy controls. Results Gene expression analysis of 84 targets indicated dysregulations in cholesterol metabolism in PXE fibroblasts. Transcript levels of ABCC6 were strongly increased in lipoprotein-deficient serum (LPDS) and under serum starvation in healthy controls. For the first time, increased HMG CoA reductase activities were found in PXE fibroblasts. We further observed strongly elevated transcript and protein levels for the proprotein convertase subtilisin/kexin type 9 (PCSK9), as well as a significant reduction in APOE mRNA expression in PXE. Conclusion Increased cholesterol biosynthesis, elevated PCSK9 levels and reduced APOE mRNA expression newly found in PXE fibroblasts could enforce atherogenesis and cardiovascular risk in PXE patients. Moreover, the increase in ABCC6 expression accompanied by the induction of cholesterol biosynthesis supposes a functional role for ABCC6 in human lipoprotein and cholesterol homeostasis.}}, author = {{Kuzaj, Patricia and Kuhn, Joachim and Dabisch-Ruthe, Mareike and Faust, Isabel and Götting, Christian and Knabbe, Cornelius and Hendig, Doris}}, booktitle = {{Lipids in Health and Disease}}, issn = {{1476-511X}}, keywords = {{Pseudoxanthoma elasticum, ABC transporter, ABCC6, Cholesterol biosynthesis, Atherosclerosis, HMG CoA reductase, SREBP2, PCSK9, LDLR, APOE}}, number = {{1}}, publisher = {{Biomed Central }}, title = {{{ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?}}}, doi = {{10.1186/1476-511x-13-118}}, volume = {{13}}, year = {{2014}}, } @misc{12987, abstract = {{Mutations in the ABC transporter ABCC6 were recently identified as cause of Pseudoxanthoma elasticum (PXE), a rare genetic disorder characterized by progressive mineralization of elastic fibers. We used an untargeted metabolic approach to identify biochemical differences between human dermal fibroblasts from healthy controls and PXE patients in an attempt to find a link between ABCC6 deficiency, cellular metabolic alterations and disease pathogenesis. 358 compounds were identified by mass spectrometry covering lipids, amino acids, peptides, carbohydrates, nucleotides, vitamins and cofactors, xenobiotics and energy metabolites. We found substantial differences in glycerophospholipid composition, leucine dipeptides, and polypeptides as well as alterations in pantothenate and guanine metabolism to be significantly associated with PXE pathogenesis. These findings can be linked to extracellular matrix remodeling and increased oxidative stress, which reflect characteristic hallmarks of PXE. Our study could facilitate a better understanding of biochemical pathways involved in soft tissue mineralization.}}, author = {{Kuzaj, Patricia and Kuhn, Joachim and Michalek, Ryan D. and Karoly, Edward D. and Faust, Isabel and Dabisch-Ruthe, Mareike and Knabbe, Cornelius and Hendig, Doris}}, booktitle = {{PLOS ONE / Public Library of Science }}, issn = {{1932-6203}}, number = {{9}}, publisher = {{Public Library of Science (PLoS)}}, title = {{{Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls}}}, doi = {{10.1371/journal.pone.0108336}}, volume = {{9}}, year = {{2014}}, } @misc{13023, abstract = {{Transducers based on dielectric electroactive polymers (DEAP) use electrostatic pressure to convert electric energy into strain energy or vice versa. Besides this, they are also designed for sensor applications in monitoring the actual stretch state on the basis of the deformation dependent capacitive-resistive behavior of the DEAP. In order to enable an efficient and proper closed loop control operation of these transducers, e.g. in positioning or energy harvesting applications, on the one hand, sensors based on DEAP material can be integrated into the transducers and evaluated externally, and on the other hand, the transducer itself can be used as a sensor, also in terms of self-sensing. For this purpose the characteristic electrical behavior of the transducer has to be evaluated in order to determine the mechanical state. Also, adequate online identification algorithms with sufficient accuracy and dynamics are required, independent from the sensor concept utilized, in order to determine the electrical DEAP parameters in real time. Therefore, in this contribution, algorithms are developed in the frequency domain for identifications of the capacitance as well as the electrode and polymer resistance of a DEAP, which are validated by measurements. These algorithms are designed for self-sensing applications, especially if the power electronics utilized is operated at a constant switching frequency, and parasitic harmonic oscillations are induced besides the desired DC value. These oscillations can be used for the online identification, so an additional superimposed excitation is no longer necessary. For this purpose a dual active bridge (DAB) is introduced to drive the DEAP transducer. The capabilities of the real-time identification algorithm in combination with the DAB are presented in detail and discussed, finally.}}, author = {{Hoffstadt, Thorben and Griese, Martin and Maas, Jürgen}}, booktitle = {{Smart Materials and Structures}}, issn = {{1361-665X}}, keywords = {{dielectric electroactive polymer, identification algorithm, self-sensing, DEAP sensor}}, location = {{Snowbird, UT}}, number = {{10}}, publisher = {{IOP Publishing}}, title = {{{Online identification algorithms for integrated dielectric electroactive polymer sensors and self-sensing concepts}}}, doi = {{10.1088/0964-1726/23/10/104007}}, volume = {{23}}, year = {{2014}}, } @misc{943, author = {{Reuther, Aljoscha}}, pages = {{76}}, publisher = {{Hochschule Ostwestfalen-Lippe}}, title = {{{Theorie und Praxis des Hörspiels}}}, year = {{2014}}, } @misc{9759, author = {{Stemmer, Boris}}, booktitle = {{Specifics - Discussing Landscape Architecture}}, editor = {{Sörensen, Christiane and Liedtke, Karoline}}, isbn = {{978-3-86859-880-3}}, location = {{Hamburg}}, pages = {{192–196}}, publisher = {{JOVIS Verlag }}, title = {{{Landscape, Livability and Happiness in Regional Development and Landscape Planning}}}, year = {{2014}}, } @book{9763, author = {{Schmidt, Catrin and Hofmann, Martin and Dunkel, Andreas}}, editor = {{Schmidt, Catrin and Dunkel, Alexander and Hofmann, Martin and Schneeberger, Eva and Hoppenstedt, Adrain and Hage, Gottfried and Stemmer, Boris and Hartz, Andrea and Saad, Sascha and Lichtenberger, Eva and Mengel, Andreas and Schwarzer, Markus and Sauer, k}}, publisher = {{Bundesamt für Naturschutz}}, title = {{{Bundesweite Übersichten}}}, volume = {{1}}, year = {{2014}}, } @book{9775, author = {{Hoppenstedt, Adrian and Hage, Gottfried and Stemmer, Boris}}, editor = {{Schmidt, Catrin and Dunkel, Alexander and Hofmann, Martin and Schneeberger, Eva and Hoppenstedt, Adrain and Hage, Gottfried and Stemmer, Boris and Hartz, Andrea and Saad, Sascha and Lichtenberger, Eva and Mengel, Andreas and Schwarzer, Markus and Sauer, Katharina}}, pages = {{60}}, publisher = {{Bundesamt für Naturschutz}}, title = {{{Regionalplanung und Landschaftsrahmenplanung}}}, volume = {{2}}, year = {{2014}}, } @book{9776, author = {{Hartz, Andrea and Saad, Sascha and Lichtenberger, Eva}}, editor = {{Schmidt, Catrin and Dunkel, Alexander and Hofmann, Martin and Schneeberger, Eva and Hoppenstedt, Adrain and Hage, Gottfried and Stemmer, Boris and Hartz, Andrea and Saad, Sascha and Lichtenberger, Eva and Mengel, Andreas and Schwarzer, Markus and Sauer, Katharina }}, pages = {{64}}, publisher = {{Bundesamt für Naturschutz }}, title = {{{Energiewende als Herausforderung für die Region}}}, volume = {{3}}, year = {{2014}}, }