@book{10743, editor = {{Stosch, Martin}}, pages = {{142}}, publisher = {{Interessengemeinschaft Leichtbau e.V.}}, title = {{{igeL-Leichtbau-Handbuch / ZOW-Preview-Ausgabe 2020}}}, year = {{2020}}, } @misc{10776, author = {{Stosch, Martin and Kettler, Peter}}, pages = {{12}}, title = {{{Leichtbauplatte. (DE102019100681A1)}}}, year = {{2020}}, } @misc{10778, author = {{Stosch, Martin and Kettler, Peter}}, pages = {{11}}, title = {{{Leichtbauplatte. (WO002020144296A3)}}}, year = {{2020}}, } @misc{10779, author = {{Stosch, Martin}}, location = {{Lemgo (Schloss Brake)}}, pages = {{64 Vortragsfolien}}, title = {{{Smart Wood – Smart Wood Factory.}}}, year = {{2020}}, } @misc{9764, author = {{Ropers, Dominik and Stemmer, Boris and Steffen-Waschek, Birgit and Milde, Bernd}}, publisher = {{Technische Hochschule Ostwestfalen-Lippe}}, title = {{{Entwicklung eines zukunfts- und querschnittsorientierten Planungsinstruments durch Neuaufstellung der Landschaftspläne im Kreis Lippe: Vorstudie: Abschlussbericht}}}, year = {{2020}}, } @book{9765, author = {{Riedl, Ulrich and Stemmer, Boris and Philipper, Sven and Peters, Wolfgang and Schicketanz, Sven and Thylmann, Miron and Pape, Carsten and Gauglitz, Philip and Mülder, Jochen and Westarp, Christian and Moczek, Nicola}}, isbn = {{978-3-89624-331-7}}, publisher = {{Bundesamt für Naturschutz}}, title = {{{Szenarien für den Ausbau der erneuerbaren Energien aus Naturschutzsicht: in Vorbereitung: FKZ 3515 82 2900 UFOPLAN 2018}}}, doi = {{10.19217/skr570 }}, volume = {{570}}, year = {{2020}}, } @misc{12231, abstract = {{In temperate lakes, it is generally assumed that light rather than temperature constrains phytoplankton growth in winter. Rapid winter warming and increasing observations of winter blooms warrant more investigation of these controls. We investigated the mechanisms regulating a massive winter diatom bloom in a temperate lake. High frequency data and process-based lake modeling demonstrated that phytoplankton growth in winter was dually controlled by light and temperature, rather than by light alone. Water temperature played a further indirect role in initiating the bloom through ice-thaw, which increased light exposure. The bloom was ultimately terminated by silicon limitation and sedimentation. These mechanisms differ from those typically responsible for spring diatom blooms and contributed to the high peak biomass. Our findings show that phytoplankton growth in winter is more sensitive to temperature, and consequently to climate change, than previously assumed. This has implications for nutrient cycling and seasonal succession of lake phytoplankton communities. The present study exemplifies the strength in integrating data analysis with different temporal resolutions and lake modeling. The new lake ecological model serves as an effective tool in analyzing and predicting winter phytoplankton dynamics for temperate lakes.}}, author = {{Kong, Xiangzhen and Seewald, Michael and Dadi, Tallent and Friese, Kurt and Mi, Chenxi and Boehrer, Bertram and Schultze, Martin and Rinke, Karsten and Shatwell, Tom}}, booktitle = {{Water research : a journal of the International Water Association}}, issn = {{1879-2448}}, keywords = {{Winter diatom bloom, High frequency monitoring, Lake modeling, Light limitation, Temperature}}, publisher = {{Elsevier BV}}, title = {{{Unravelling winter diatom blooms in temperate lakes using high frequency data and ecological modeling}}}, doi = {{10.1016/j.watres.2020.116681}}, volume = {{190}}, year = {{2020}}, } @misc{12233, abstract = {{The thermal structure in reservoirs affects the development of aquatic ecosystems, and can be substantially influenced by climate change and management strategies. We applied a two-dimensional hydrodynamic model to explore the response of the thermal structure in Germany's largest drinking water reservoir, Rappbode Reservoir, to future climate projections and different water withdrawal strategies. We used projections for representative concentration pathways (RCP) 2.6, 6.0 and 8.5 from an ensemble of 4 different global climate models. Simulation results showed that epilimnetic water temperatures in the reservoir strongly increased under all three climate scenarios. Hypolimnetic temperatures remained rather constant under RCP 2.6 and RCP 6.0 but increased markedly under RCP 8.5. Under the intense warming in RCP 8.5, hypolimnion temperatures were projected to rise from 5 °C to 8 °C by the end of the century. Stratification in the reservoir was projected to be more stable under RCP 6.0 and RCP 8.5, but did not show significant changes under RCP 2.6. Similar results were found with respect to the light intensity within the mixed-layer. Moreover, the results suggested that surface withdrawal can be an effective adaptation strategy under strong climate warming (RCP 8.5) to reduce surface warming and avoid hypolimnetic warming. This study documents how global scale climate projections can be translated into site-specific climate impacts to derive adaptation strategies for reservoir operation. Moreover, our results illustrate that the most intense warming scenario, i.e. RCP 8.5, demands far-reaching climate adaptation while the mitigation scenario (RCP 2.6) does not require adaptation of reservoir management before 2100.}}, author = {{Mi, Chenxi and Shatwell, Tom and Ma, Jun and Xu, Yaqian and Su, Fangli and Rinke, Karsten}}, booktitle = {{The science of the total environment : an international journal for scientific research into the environment and its relationship with man}}, issn = {{1879-1026}}, keywords = {{Rappbode Reservoir, Thermal structure, Climate change, CE-QUAL-W2, Selective water withdrawal}}, number = {{12}}, publisher = {{Elsevier BV}}, title = {{{Ensemble warming projections in Germany's largest drinking water reservoir and potential adaptation strategies}}}, doi = {{10.1016/j.scitotenv.2020.141366}}, volume = {{748}}, year = {{2020}}, } @misc{12234, author = {{Kyba, Christopher C. M. and Conrad, Jeff and Shatwell, Tom}}, booktitle = {{Nature ecology & evolution}}, issn = {{2397-334X}}, number = {{3}}, pages = {{318--319}}, publisher = {{Nature Publishing Group }}, title = {{{Lunar illuminated fraction is a poor proxy for moonlight exposure}}}, doi = {{10.1038/s41559-020-1096-7}}, volume = {{4}}, year = {{2020}}, } @misc{12235, abstract = {{Metalimnetic oxygen minima are observed in many lakes and reservoirs, but the mechanisms behind this phenomena are not well understood. Thus, we simulated the metalimnetic oxygen minimum (MOM) in the Rappbode Reservoir with a well-established two-dimensional water quality model (CE-QUAL-W2) to systematically quantify the chain of events leading to its formation. We used high-resolution measured data to calibrate the model, which accurately reproduced the physical (e.g. water level and water temperature), biogeochemical (e.g. nutrient and oxygen dynamics) and ecological (e.g. algal community dynamics) features of the reservoir, particularly the spatial and temporal extent of the MOM. The results indicated that around 60% of the total oxygen consumption rate in the MOM layer originated from benthic processes whereas the remainder originated from pelagic processes. The occurrence of the cyanobacterium Planktothrix rubescens in the metalimnion delayed and slightly weakened the MOM through photosynthesis, although its decaying biomass ultimately induced the MOM. Our research also confirmed the decisive role of water temperature in the formation of the MOM since the water temperatures, and thus benthic and pelagic oxygen consumption rates, were higher in the metalimnion than in the hypolimnion. Our model is not only providing novel conclusions about the drivers of MOM development and their quantitative contributions, it is also a new tool for understanding and predicting ecological and biogeochemical water quality dynamics.}}, author = {{Mi, Chenxi and Shatwell, Tom and Ma, Jun and Wentzky, Valerie Carolin and Boehrer, Bertram and Xu, Yaqian and Rinke, Karsten}}, booktitle = {{Water research : a journal of the International Water Association}}, issn = {{1879-2448}}, keywords = {{Rappbode reservoir, CE-QUAL-W2, Planktothrix rubescens, Metalimnion, Oxygen consumption, Benthic processes}}, number = {{5}}, publisher = {{Elsevier BV}}, title = {{{The formation of a metalimnetic oxygen minimum exemplifies how ecosystem dynamics shape biogeochemical processes: A modelling study}}}, doi = {{10.1016/j.watres.2020.115701}}, volume = {{175}}, year = {{2020}}, } @phdthesis{12717, abstract = {{Der Anstieg der Komplexität in Produktionssystemen stellt eine der größten Herausforderungen für produzierende Unternehmen dar. In der vorliegenden Dissertation werden mögliche Auswirkungen von Komplexität auf die Performance von Produktionsgruppen untersucht. Es werden sechs Studien durchgeführt, die inhaltlich und methodisch aufeinander aufbauen und somit einen vollständigen Forschungsprozess von der initialen Begriffsbestimmung bis hin zur Ableitung von Gestaltungsempfehlungen für die betriebliche Praxis beschreiben. In der Explorationsphase wird zunächst eine qualitative Interviewstudie durchgeführt, um konkrete Treiber für das Phänomen der Komplexität in digitalisierten Arbeitssystemen zu identifizieren. Basierend auf zwei systematischen Literaturstudien wird der Stand der Forschung zu (1) Komplexitätsmodellen und (2) Gruppenperformancemodellen im Kontext von Produktionssystemen strukturiert erarbeitet und das bestehende Forschungsdefizit offengelegt. Auf der Grundlage der Vorstudien wird ein hypothetisches Modell zur Erklärung der Performance von Produktionsgruppen entwickelt, das neben bekannten Einflussgrößen aus der Teamforschung verschiedene Komplexitätsdimensionen berücksichtigt. Die Operationalisierung der latenten Variablen erfolgt mit Hilfe einer weiteren qualitativen Interviewstudie. Das Gruppenperformancemodell wird in einer Fragebogenstudie (n = 455) unter Anwendung der Methode der Strukturgleichungsmodellierung empirisch evaluiert. In der statistischen Auswertung wird u. a. festgestellt, dass ein positiver Zusammenhang zwischen der Aufgabenkomplexität und der Gruppenperformance besteht, der über die Qualität der Zusammenarbeit vermittelt wird. Dahingegen wird ein negativer Zusammenhang zwischen der Komplexität des Gruppendesigns und der Gruppenperformance identifiziert, der ebenfalls über die Qualität der Zusammenarbeit vermittelt wird. Aus den Ergebnissen werden konkrete Empfehlungen für die komplexitätsbezogene Gestaltung von Gruppenarbeit in der Produktion abgeleitet. Darüber hinaus wird eine Metrik zur Erfassung der Komplexität des Arbeitsprozesses von Produktionsgruppen entwickelt und in einer Simulationsstudie evaluiert. Die Metrik kann bereits in Planungsphasen von Produktionssystemen verwendet werden, um alternative arbeitsorganisatorische Gestaltungsoptionen anhand des resultierenden Komplexitätsniveaus für die Produktionsgruppe vergleichend zu analysieren. Zudem können die Auswirkungen unterschiedlicher Qualifikationsprofile auf das Komplexitätsniveau und die Performance einer Produktionsgruppe untersucht werden.}}, author = {{Latos, Benedikt}}, isbn = {{978-3-8440-7744-5}}, keywords = {{Produktionssystem, Komplexes System, Teamwork, Leistungsmessung}}, pages = {{324}}, publisher = {{Shaker Verlag}}, title = {{{Auswirkungen von Komplexität auf die Performance von Produktionsgruppen}}}, doi = {{10.2370/9783844077445}}, volume = {{37}}, year = {{2020}}, } @misc{12787, abstract = {{Vapor phase hydrogen peroxide (H2O2) can be utilized to inactivate murine norovirus (MNV), a surrogate of human norovirus, on surface areas. However, vapor phase H2O2 inactivation of virus on fruits and vegetables has not been characterized. In this study, MNV was used to determine whether vaporized H2O2 inactivates virus on surfaces of various fruits and vegetables (apples, blueberries, cucumbers, and strawberries). The effect of vapor phase H2O2 decontamination was investigated with two application systems. Plaque assays were performed after virus recovery from untreated and treated fresh produce to compare the quantity of infective MNV. The Mann-Whitney U test was applied to the test results to evaluate the virus titer reductions of treated food samples, with significance set at P <= 0.05. The infective MNV populations were significantly reduced on smooth surfaces by 4.3 log PFU (apples, P < 0.00001) and 4 log PFU or below the detection limit (blueberries, P = 0.0074) by treatment with vapor phase H2O2 (60 min, maximum of 214 ppm of H2O2). Similar treatments of artificially contaminated cucumbers resulted in a virus titer reduction of 1.9 log PFU. Treatment of inoculated strawberries resulted in 0.1and 2.8-log reductions of MNV. However, MNV reduction rates on cucumbers (P = 0.3809) and strawberries (P = 0,7414) were not significant. Triangle tests and color measurements of untreated and treated apples, cucumbers, blueberries, and strawberries revealed no differences in color and consistency after H2O2 treatment. No increase of the H2O2 concentration in treated fruits and vegetables compared with untreated produce was observed. This study reveals for the first time the conditions under which vapor phase H2O2 inactivates MNV on selected fresh fruit and vegetable surfaces.}}, author = {{Becker, Barbara and Dabisch-Ruthe, Mareike and Pfannebecker, Jens}}, booktitle = {{ Journal of food protection }}, issn = {{1944-9097}}, keywords = {{Fruits, Inactivation, Murine norovirus, Vapor phase hydrogen peroxide, Vegetables}}, number = {{1}}, pages = {{45--51}}, publisher = {{IAFP}}, title = {{{Inactivation of Murine Norovirus on Fruit and Vegetable Surfaces by Vapor Phase Hydrogen Peroxide}}}, doi = {{10.4315/0362-028X.JFP-19-238}}, volume = {{83}}, year = {{2020}}, } @misc{12794, abstract = {{Modern product development utilizes both Product Lifecycle Management (PLM) and Application Lifecycle Management (ALM). PLM addresses the hardware lifecycle of a product, whereas ALM addresses the software lifecycle. In recent years, industry and academia have developed several PLM/ALM integration concepts to realize efficient management of the product lifecycle across all domains. However, the solutions available in practice are typically vendor-driven. Therefore, they are not generally applicable even if standards such as OSLC (Open Services for Lifecycle Cooperation) are applied. The consortium "Plattform Industrie 4.0" has recently introduced a standardized digital representation of an asset (e.g., a smart product): the Asset Administration Shell (AAS). The AAS has the potential to integrate PLM/ALM data sets in a single product model and hence to provide a generally applicable interface for PLM/ALM integration. However, until now there has not been a concept to prove this potential. The aim of this work is to develop such new strategies (named Plm4AAS) using AAS submodels. This article explains the semi-automatic generation of PLM/ALM submodels and how to link elements between these submodels. The AASX Package Explorer, an AAS management software tool, is used to demonstrate the results. The article finishes with a discussion about the potential of the AAS as a standardized concept for PLM/ALM integration. (C) 2020 The Authors. Published by Elsevier B.V. Peer-review under responsibility of the scientific committee of the 5th International Conference on System-Integrated Intelligence.}}, author = {{Deuter, Andreas and Imort, Sebastian}}, booktitle = {{System-Integrated Intelligence - Intelligent, Flexible and Connected Systems in Products and Production : Proceedings of the 5th International Conference on System-Integrated Intelligence (SysInt 2020), Bremen, Germany (}}, editor = {{Thoben, Klaus-Dieter and Dekena, Berend and Lang, Walter and Trächtler, Ansgar}}, issn = {{2351-9789}}, keywords = {{PLM, ALM, ReqIF, PLM XML, OSLC, Asset Administration Shell}}, location = {{Online}}, pages = {{234--240}}, publisher = {{Elsevier BV}}, title = {{{PLM/ALM Integration With The Asset Administration Shell}}}, doi = {{10.1016/j.promfg.2020.11.040}}, volume = {{52}}, year = {{2020}}, } @misc{12807, abstract = {{Writing chorales in the style of Bach has been a music theory exercise for generations of music students. As such it is not surprising that automatic Bach chorale harmonization has been a topic in music technology for decades. We suggest several improvements to current neural network solutions based on musicological insights into human choral composition practices. Evaluations with expert listeners show that the generated chorales closely resemble Bach's harmonization style.}}, author = {{Leemhuis, Alexander and Waloschek, Simon and Hadjakos, Aristotelis}}, booktitle = {{Machine Learning and Knowledge Discovery in Databases : International Workshops of ECML PKDD 2019}}, editor = {{Cellier, Peggy and Driessens, Kurt}}, isbn = {{978-3-030-43886-9}}, issn = {{1865-0937}}, keywords = {{Bach chorale harmonization, Deep learning, Beam search}}, location = {{Würzburg}}, pages = {{462–469}}, publisher = {{Springer International Publishing}}, title = {{{Bacher than Bach? On Musicologically Informed AI-Based Bach Chorale Harmonization}}}, doi = {{10.1007/978-3-030-43887-6_39}}, volume = {{1168}}, year = {{2020}}, } @misc{12812, abstract = {{Discerning unexpected from expected data patterns is the key challenge of anomaly detection. Although a multitude of solutions has been applied to this modern Industry 4.0 problem, it remains an open research issue to identify the key characteristics subjacent to an anomaly, sc. generate hypothesis as to why they appear. In recent years, machine learning models have been regarded as universal solution for a wide range of problems. While most of them suffer from non-self-explanatory representations, Gaussian Processes (GPs) deliver interpretable and robust statistical data models, which are able to cope with unreliable, noisy, or partially missing data. Thus, we regard them as a suitable solution for detecting and appropriately representing anomalies and their respective characteristics. In this position paper, we discuss the problem of automatic and interpretable anomaly detection by means of GPs. That is, we elaborate on why GPs are well suited for anomaly detection and what the current challenges are when applying these probabilistic models to large-scale production data.}}, author = {{Berns, Fabian and Lange-Hegermann, Markus and Beecks, Christian}}, booktitle = {{ Proceedings of the International Conference on Innovative Intelligent Industrial Production and Logistics IN4PL - Volume 1}}, editor = {{Panetto, H. and Madani, K. and Smirnov, A.}}, isbn = {{978-989-758-476-3}}, keywords = {{Anomaly Detection, Gaussian Processes, Explainable Machine Learning, Industry 4.0}}, location = {{Budapest, HUNGARY}}, pages = {{87--92}}, publisher = {{SCITEPRESS - Science and Technology Publications}}, title = {{{Towards Gaussian Processes for Automatic and Interpretable Anomaly Detection in Industry 4.0}}}, doi = {{10.5220/0010130300870092}}, year = {{2020}}, } @misc{12835, abstract = {{Delayed-release dosage forms are mainly manufactured as batch processes and include coated tablets, pellets, or particles with gastric resistant polymers. Authors propose a novel approach using the hot-melt extrusion technique to prepare delayed release dosage forms via a continuous manufacturing process, a new trend in the pharmaceutical industry. A full factorial design was employed to correlate input variables, including stearic acid (SA) content, drug content, and pellet size with drug release properties of the pellets. PLS fit method suitably elaborated the relationship between input and output variables with reasonably good fit and goodness of prediction. All three input factors influenced drug release in enzyme-free simulated gastric fluid (SGF) after 120 min; however, SA content did not significantly affect drug dissolution in the enzyme-free simulated intestinal fluid (SIF). An optimized formulation and design space were determined by overlaying multiple contours established from regression equations. The continuous manufacturing process was successfully monitored using inline near-infrared (NIR) and inline particle size analysis, with drug load and pellet size being well-controlled within the design space. The obtained pellets released less than 5% after 120 min in SGF and more than 85% and 95% after 30 min and 45 min, respectively, after switching to SIF. (C) 2020 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.}}, author = {{Vo, Anh Q. and Kutz, Gerd and He, Herman and Narala, Sagar and Bandari, Suresh and Repka, Michael A.}}, booktitle = {{Journal of Pharmaceutical Sciences}}, issn = {{1520-6017}}, keywords = {{Continuous manufacturing, Delayed-release, FT-NIR, Inline particle size analysis, Hot melt extrusion}}, number = {{12}}, pages = {{3598--3607}}, publisher = {{Elsevier BV}}, title = {{{Continuous Manufacturing of Ketoprofen Delayed Release Pellets Using Melt Extrusion Technology: Application of QbD Design Space, Inline Near Infrared, and Inline Pellet Size Analysis}}}, doi = {{10.1016/j.xphs.2020.09.007}}, volume = {{109}}, year = {{2020}}, } @misc{12857, abstract = {{On the occasion of the 250th anniversary celebrations of Ludwig van Beethoven in 2020, we developed the musical room installation "Inside Beethoven! The Audience Goes on Stage". It is a stage with virtual musicians. Visitors are invited to enter, take the position of the musicians and listen to each of them at close range. The installation emulates the listening perspective of the musicians, thus, providing an uncommon experience to visitors who are mostly used to listen to music from recordings or from a concert audience's perspective. The musicians play Beethoven's septet op. 20 and its trio arrangement op. 38, two exclusive 14 channel music productions. Both versions are aligned and can be switched seamlessly. Digital music stands visualize the score. In this paper we trace the genesis and construction of the installation and point at some conceptual and technical challenges tackled.}}, author = {{Berndt, Axel and Waloschek, Simon and Hadjakos, Aristotelis and Veit, Joachim}}, booktitle = {{Proceedings of the 15th International Audio Mostly Conference}}, editor = {{Groß-Vogt, Katharina }}, isbn = {{978-1-4503-7563-4}}, keywords = {{Beethoven, Music, Artistic Installation}}, location = {{Graz, AUSTRIA}}, pages = {{245--248}}, publisher = {{ACM}}, title = {{{Inside Beethoven!}}}, doi = {{10.1145/3411109.3411115}}, year = {{2020}}, } @misc{12955, abstract = {{Ein 10 Monate alter, männlich intakter Rhodesian Ridgeback wurde wegen chronischen Dickdarmdurchfalls und Hämatochezie vorgestellt. Der Hund stammte aus Deutschland und hatte das Land nie verlassen. Die Laboruntersuchung des vorbehandelnden Tierarztes ergab neben einer Neutrophilie eine Hyperkaliämie und eine Hyponatriämie. Mit einem Serumbasalkortisolwert von 4,3 µg/dl konnte ein Hypoadrenokortizismus weitgehend ausgeschlossen werden. Eine vom Tierarzt durch geführte antibiotische Behandlung hatte keine Besserung bewirkt. Daher war der Hund mit Prednisolon behandelt worden. Unter 2-wöchiger Prednisolongabe kam es zu einer deutlichen Verstärkung des Durchfalls sowie einem Gewichtsverlust von 6 kg. Bei Vorstellung an der Medizinischen Kleintierklinik der LMU München war der Hund im Allgemeinbefinden mittelgradig reduziert, deutlich abgemagert, dehydriert, hypovolämisch und hatte eine rektale Körpertemperatur von 39,6 °C. Bei der sonografischen Untersuchung zeigte sich eine generalisiert verdickte Dickdarmwand und koloskopisch eine hochgradig ulzerativ veränderte Dickdarmschleimhaut. Histologischer Befund war eine ulzerative granulomatöse Kolitis. Durch die Periodic-Acid-Schiff-Reaktion ließen sich in den Schnitten der Dickdarmbioptate mikrobielle Strukturen darstellen, die für eine Algeninfektion diagnostisch waren. Die bei der mikrobiellen Untersuchung anzüchtbaren Prototheken wurden mittels MALDI-TOF-MS als Prototheca zopfii identifiziert. Zum Nachweis einer möglichen Immundefizienz wurden die Immunglobuline im Serum bestimmt. Die IgM-Konzentration war erniedrigt, während sich IgG- und IgA-Konzentration im Referenzbereich befanden. Aufgrund der Verschlechterung des Allgemeinbefindens, der vorsichtigen Prognose und der hohen Kosten eines Therapieversuchs wurde der Hund eine Woche später euthanasiert und der Tierkörper pathologisch untersucht. Histopathologisch wurden Prototheken auch in den abdominalen Lymphknoten, jedoch nicht in den Augen oder im zentralen Nervensystem identifiziert. Der Fall zeigt, dass eine Prototheken-Infektion auch bei Hunden aus Deutschland als Differenzialdiagnose für chronischen Dickdarmdurchfall in Betracht gezogen werden sollte, insbesondere bei Patienten mit ulzerativer granulomatöser Kolitis. Sie kann bei der histologischen Untersuchung ohne Spezialfärbung leicht übersehen werden.}}, author = {{Geisen, Vera and Mayer, Christian and Harrer, Julia and Hartmann, Katrin and Ulrich, Sebastian and Unterer, Stefan}}, booktitle = {{Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere}}, issn = {{2567-5842}}, keywords = {{Hund - Algeninfektion - chronischer Durchfall - Protothekose - Prototheca zopfii}}, number = {{05}}, pages = {{369--375}}, publisher = {{Thieme }}, title = {{{Ulzerative granulomatöse Kolitis durch Prototheca spp. bei einem Rhodesian Ridgeback in Deutschland}}}, doi = {{10.1055/a-1238-1554}}, volume = {{48}}, year = {{2020}}, } @misc{12956, abstract = {{Stachybotrys (S.) chartarum had been linked to severe health problems in humans and animals, which occur after exposure to the toxic secondary metabolites of this mold. S. chartarum had been isolated from different environmental sources, ranging from culinary herbs and improperly stored fodder to damp building materials. To access the pathogenic potential of isolates, it is essential to analyze them under defined conditions that allow for the production of their toxic metabolites. All Stachybotrys species are assumed to produce the immunosuppressive phenylspirodrimanes, but the highly cytotoxic macrocyclic trichothecenes are exclusively generated by the genotype S of S. chartarum. In this study, we have analyzed four genotype S strains initially isolated from three different habitats. We grew them on five commonly used media (malt-extract-agar, glucose-yeast-peptone-agar, potato-dextrose-agar, cellulose-agar, Sabouraud-dextrose-agar) to identify conditions that promote mycotoxin production. Using LC-MS/MS, we have quantified stachybotrylactam and all S-type specific macrocyclic trichothecenes (satratoxin G, H, F, roridin E, L-2, verrucarin J). All five media supported a comparable fungal growth and sporulation at 25 °C in the dark. The highest concentrations of macrocyclic trichothecenes were detected on potato-dextrose-agar or cellulose-agar. Malt-extract-agar let to an intermediate and glucose-yeast-peptone-agar and Sabouraud-dextrose-agar to a poor mycotoxin production. These data demonstrate that the mycotoxin production clearly depends on the composition of the respective medium. Our findings provide a starting point for further studies in order to identify individual components that either support or repress the production of mycotoxins in S. chartarum.}}, author = {{Ulrich, Sebastian and Schäfer, Cornelius}}, booktitle = {{Journal of Fungi}}, issn = {{2309-608X}}, keywords = {{Stachybotrys, genotype, macrocyclic trichothecenes, stachybotrylactam}}, number = {{3}}, publisher = {{MDPI }}, title = {{{Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media}}}, doi = {{10.3390/jof6030159}}, volume = {{6}}, year = {{2020}}, } @misc{12962, abstract = {{Background Borrelia burgdorferi is a tick-borne spirochete that causes Lyme borreliosis (LB). After an initial tick bite, it spreads from the deposition site in the dermis to distant tissues of the host. It is generally believed that this spirochete disseminates via the hematogenous route. Borrelia persica causes relapsing fever and is able to replicate in the blood stream. Currently the exact dissemination pathway of LB pathogens in the host is not known and controversially discussed. Methods In this study, we established a strict intravenous infection murine model using host-adapted spirochetes. Survival capacity and infectivity of host-adapted B. burgdorferi sensu stricto (Bbss) were compared to those of B. persica (Bp) after either intradermal (ID) injection into the dorsal skin of immunocompetent mice or strict intravenous (IV) inoculation via the jugular vein. By in vitro culture and PCR, viable spirochetes and their DNA load in peripheral blood were periodically monitored during a 49/50-day course post-injection, as well as in various tissue samples collected at day 49/50. Specific antibodies in individual plasma/serum samples were detected with serological methods. Results Regardless of ID or IV injection, DNA of Bp was present in blood samples up to day 24 post-challenge, while no Bbss was detectable in the blood circulation during the complete observation period. In contrast to the brain tropism of Bp, Bbss spirochetes were found in ear, skin, joint, bladder, and heart tissue samples of only ID-inoculated mice. All tested tissues collected from IV-challenged mice were negative for traces of Bbss. ELISA testing of serum samples showed that Bp induced gradually increasing antibody levels after ID or IV inoculation, while Bbss did so only after ID injection but not after IV inoculation. Conclusions This study allows us to draw the following conclusions: (i) Bp survives in the blood and disseminates to the host’s brain via the hematogenous route; and (ii) Bbss, in contrast, is cleared rapidly from the blood stream and is a tissue-bound spirochete.}}, author = {{Liang, Liucun and Wang, Jinyong and Schorter, Lucas and Nguyen Trong, Thu Phong and Fell, Shari and Ulrich, Sebastian and Straubinger, Reinhard K.}}, booktitle = {{Parasites & vectors}}, issn = {{1756-3305}}, keywords = {{Lyme borreliosis, Borrelia burgdorferi, Tick-borne relapsing fever, Borrelia persica, Blood clearance}}, number = {{1}}, publisher = {{BioMed Central }}, title = {{{Rapid clearance of Borrelia burgdorferi from the blood circulation}}}, doi = {{10.1186/s13071-020-04060-y}}, volume = {{13}}, year = {{2020}}, } @misc{12963, abstract = {{The genus Borrelia comprises vector-borne bacterial pathogens that can severely affect human and animal health. Members of the Borrelia burgdorferi sensu lato species complex can cause Lyme borreliosis, one of the most common vector-borne diseases in the Northern hemisphere. Besides, members of the relapsing fever group of spirochetes can cause tick-borne relapsing fever in humans and various febrile illnesses in animals in tropical, subtropical and temperate regions. Borrelia spp. organisms are fastidious to cultivate and to maintain in vitro, and therefore, difficult to work with in the laboratory. Currently, borrelia identification is mainly performed using PCR and DNA sequencing methods, which can be complicated/frustrating on complex DNA templates and may still be relatively expensive. Alternative techniques such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) are not well established for Borrelia spp., although this technique is currently one of the most used techniques for rapid identification of bacteria in microbiological diagnostic laboratories. This is mainly due to unsatisfactory results obtained by use of simple sample preparation techniques and medium-contamination obscuring the mass spectra. In addition, comprehensive libraries for Borrelia spp. MALDI-TOF MS have yet to be established. In this study, we developed a new filter-based chemical extraction technique that allows measurement of high quality Borrelia spp. spectra from less than 100,000 bacteria per spot in MALDI-TOF MS. We used 49 isolates of 13 different species to produce the largest mass-library for Borrelia spp. so far and to validate the protocol. The library was successfully established and identifies >96% of used isolates correctly to species level. Cluster analysis on the sum spectra was applied to all the different isolates, which resulted in tight cluster generation for most species. Comparative analysis of the generated cluster to a phylogeny based on concatenated multi-locus sequence typing genes provided a surprising homology. Our data demonstrate that the technique described here can be used for fast and reliable species and strain typing within the borrelia complex.}}, author = {{Neumann-Cip, Anna-Cathrine and Fingerle, Volker and Margos, Gabriele and Straubinger, Reinhard K. and Overzier, Evelyn and Ulrich, Sebastian and Wieser, Andreas}}, booktitle = {{Frontiers in Microbiology}}, issn = {{1664-302X}}, keywords = {{Borrelia burgdorferi sensu lato, MALDI-TOF MS, typing, sample preparation, MALDI-TOF MS library, strain typing, automatic identification}}, publisher = {{Frontiers Media SA}}, title = {{{A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation}}}, doi = {{10.3389/fmicb.2020.00690}}, volume = {{11}}, year = {{2020}}, } @misc{12964, abstract = {{Antibodies represent an important element in the adaptive immune response and a major tool to eliminate microbial pathogens. For many bacterial and viral infections, efficient vaccines exist, but not for fungal pathogens. For a long time, antibodies have been assumed to be of minor importance for a successful clearance of fungal infections; however this perception has been challenged by a large number of studies over the last three decades. In this review, we focus on the potential therapeutic and prophylactic use of monoclonal antibodies. Since systemic mycoses normally occur in severely immunocompromised patients, a passive immunization using monoclonal antibodies is a promising approach to directly attack the fungal pathogen and/or to activate and strengthen the residual antifungal immune response in these patients.}}, author = {{Ulrich, Sebastian and Ebel, Frank}}, booktitle = {{Journal of Fungi}}, issn = {{2309-608X}}, keywords = {{monoclonal antibodies, invasive fungal infections, therapy, prophylaxis, opsonization}}, number = {{1}}, publisher = {{MDPI }}, title = {{{Monoclonal Antibodies as Tools to Combat Fungal Infections}}}, doi = {{10.3390/jof6010022}}, volume = {{6}}, year = {{2020}}, }