{"date_updated":"2024-06-26T13:55:06Z","year":"2010","date_created":"2024-03-16T19:35:23Z","doi":"10.1007/s00216-010-4446-5","status":"public","type":"scientific_journal_article","volume":399,"abstract":[{"lang":"eng","text":"Stopped-flow technology is frequently used to monitor rapid (bio)chemical reactions with high temporal resolution, e.g., in dynamic investigations of enzyme reactions, protein interactions, or molecular transport mechanisms. However, conventional stopped-flow devices are often overly complex, voluminous, or costly. Moreover, excessive amounts of sample are often wasted owing to inefficient designs. To address these shortcomings, we propose a stopped-flow system based on microfluidic design principles. Our simple and cost-efficient approach offers distinct advantages over existing technology. In particular, the use of injection-molded disposable microfluidic chips minimizes required sample volumes and associated costs, simplifies handling, and prevents adverse cross-contamination effects. The cost of the system developed is reduced by an order of magnitude compared with the cost of commercial systems. The system contains a high-precision valve system for fluid control and features automated data acquisition capability with high temporal resolution. Analyses with two well-established reaction kinetics yielded a dead time of approximately 8-9 ms."}],"_id":"11223","intvolume":" 399","user_id":"65129","title":"Compact, cost-efficient microfluidics-based stopped-flow device","publication":"Analytical and bioanalytical chemistry","page":"1117–1125","publisher":"Springer","language":[{"iso":"eng"}],"place":"Berlin","author":[{"full_name":"Bleul, Regina","last_name":"Bleul","first_name":"Regina"},{"full_name":"Ritzi-Lehnert, Marion","last_name":"Ritzi-Lehnert","first_name":"Marion"},{"last_name":"Höth","full_name":"Höth, Julian","first_name":"Julian"},{"first_name":"Nico","last_name":"Scharpfenecker","full_name":"Scharpfenecker, Nico"},{"last_name":"Frese","full_name":"Frese, Ines","first_name":"Ines"},{"last_name":"Düchs","full_name":"Düchs, Dominik","first_name":"Dominik"},{"last_name":"Brunklaus","full_name":"Brunklaus, Sabine","first_name":"Sabine"},{"full_name":"Hansen-Hagge, Thomas","last_name":"Hansen-Hagge","first_name":"Thomas"},{"first_name":"Franz-Josef","full_name":"Meyer-Almes, Franz-Josef","last_name":"Meyer-Almes"},{"first_name":"Klaus S.","last_name":"Drese","full_name":"Drese, Klaus S."}],"publication_status":"published","citation":{"ama":"Bleul R, Ritzi-Lehnert M, Höth J, et al. Compact, cost-efficient microfluidics-based stopped-flow device. Analytical and bioanalytical chemistry. 2010;399:1117-1125. doi:10.1007/s00216-010-4446-5","apa":"Bleul, R., Ritzi-Lehnert, M., Höth, J., Scharpfenecker, N., Frese, I., Düchs, D., Brunklaus, S., Hansen-Hagge, T., Meyer-Almes, F.-J., & Drese, K. S. (2010). Compact, cost-efficient microfluidics-based stopped-flow device. Analytical and Bioanalytical Chemistry, 399, 1117–1125. https://doi.org/10.1007/s00216-010-4446-5","havard":"R. Bleul, M. Ritzi-Lehnert, J. Höth, N. Scharpfenecker, I. Frese, D. Düchs, S. Brunklaus, T. Hansen-Hagge, F.-J. Meyer-Almes, K.S. Drese, Compact, cost-efficient microfluidics-based stopped-flow device, Analytical and Bioanalytical Chemistry. 399 (2010) 1117–1125.","van":"Bleul R, Ritzi-Lehnert M, Höth J, Scharpfenecker N, Frese I, Düchs D, et al. Compact, cost-efficient microfluidics-based stopped-flow device. Analytical and bioanalytical chemistry. 2010;399:1117–25.","bjps":"Bleul R et al. (2010) Compact, Cost-Efficient Microfluidics-Based Stopped-Flow Device. Analytical and bioanalytical chemistry 399, 1117–1125.","ufg":"Bleul, Regina u. a.: Compact, cost-efficient microfluidics-based stopped-flow device, in: Analytical and bioanalytical chemistry 399 (2010), S. 1117–1125.","short":"R. Bleul, M. Ritzi-Lehnert, J. Höth, N. Scharpfenecker, I. Frese, D. Düchs, S. Brunklaus, T. Hansen-Hagge, F.-J. Meyer-Almes, K.S. Drese, Analytical and Bioanalytical Chemistry 399 (2010) 1117–1125.","ieee":"R. Bleul et al., “Compact, cost-efficient microfluidics-based stopped-flow device,” Analytical and bioanalytical chemistry, vol. 399, pp. 1117–1125, 2010, doi: 10.1007/s00216-010-4446-5.","chicago":"Bleul, Regina, Marion Ritzi-Lehnert, Julian Höth, Nico Scharpfenecker, Ines Frese, Dominik Düchs, Sabine Brunklaus, Thomas Hansen-Hagge, Franz-Josef Meyer-Almes, and Klaus S. Drese. “Compact, Cost-Efficient Microfluidics-Based Stopped-Flow Device.” Analytical and Bioanalytical Chemistry 399 (2010): 1117–25. https://doi.org/10.1007/s00216-010-4446-5.","chicago-de":"Bleul, Regina, Marion Ritzi-Lehnert, Julian Höth, Nico Scharpfenecker, Ines Frese, Dominik Düchs, Sabine Brunklaus, Thomas Hansen-Hagge, Franz-Josef Meyer-Almes und Klaus S. Drese. 2010. Compact, cost-efficient microfluidics-based stopped-flow device. Analytical and bioanalytical chemistry 399: 1117–1125. doi:10.1007/s00216-010-4446-5, .","din1505-2-1":"Bleul, Regina ; Ritzi-Lehnert, Marion ; Höth, Julian ; Scharpfenecker, Nico ; Frese, Ines ; Düchs, Dominik ; Brunklaus, Sabine ; Hansen-Hagge, Thomas ; u. a.: Compact, cost-efficient microfluidics-based stopped-flow device. In: Analytical and bioanalytical chemistry Bd. 399. Berlin, Springer (2010), S. 1117–1125","mla":"Bleul, Regina, et al. “Compact, Cost-Efficient Microfluidics-Based Stopped-Flow Device.” Analytical and Bioanalytical Chemistry, vol. 399, 2010, pp. 1117–25, https://doi.org/10.1007/s00216-010-4446-5."},"publication_identifier":{"eissn":["1618-2650"],"issn":["1618-2642 "]}}