---
_id: '11223'
abstract:
- lang: eng
  text: Stopped-flow technology is frequently used to monitor rapid (bio)chemical
    reactions with high temporal resolution, e.g., in dynamic investigations of enzyme
    reactions, protein interactions, or molecular transport mechanisms. However, conventional
    stopped-flow devices are often overly complex, voluminous, or costly. Moreover,
    excessive amounts of sample are often wasted owing to inefficient designs. To
    address these shortcomings, we propose a stopped-flow system based on microfluidic
    design principles. Our simple and cost-efficient approach offers distinct advantages
    over existing technology. In particular, the use of injection-molded disposable
    microfluidic chips minimizes required sample volumes and associated costs, simplifies
    handling, and prevents adverse cross-contamination effects. The cost of the system
    developed is reduced by an order of magnitude compared with the cost of commercial
    systems. The system contains a high-precision valve system for fluid control and
    features automated data acquisition capability with high temporal resolution.
    Analyses with two well-established reaction kinetics yielded a dead time of approximately
    8-9 ms.
author:
- first_name: Regina
  full_name: Bleul, Regina
  last_name: Bleul
- first_name: Marion
  full_name: Ritzi-Lehnert, Marion
  last_name: Ritzi-Lehnert
- first_name: Julian
  full_name: Höth, Julian
  last_name: Höth
- first_name: Nico
  full_name: Scharpfenecker, Nico
  last_name: Scharpfenecker
- first_name: Ines
  full_name: Frese, Ines
  last_name: Frese
- first_name: Dominik
  full_name: Düchs, Dominik
  last_name: Düchs
- first_name: Sabine
  full_name: Brunklaus, Sabine
  last_name: Brunklaus
- first_name: Thomas
  full_name: Hansen-Hagge, Thomas
  last_name: Hansen-Hagge
- first_name: Franz-Josef
  full_name: Meyer-Almes, Franz-Josef
  last_name: Meyer-Almes
- first_name: Klaus S.
  full_name: Drese, Klaus S.
  last_name: Drese
citation:
  ama: Bleul R, Ritzi-Lehnert M, Höth J, et al. Compact, cost-efficient microfluidics-based
    stopped-flow device. <i>Analytical and bioanalytical chemistry</i>. 2010;399:1117-1125.
    doi:<a href="https://doi.org/10.1007/s00216-010-4446-5">10.1007/s00216-010-4446-5</a>
  apa: Bleul, R., Ritzi-Lehnert, M., Höth, J., Scharpfenecker, N., Frese, I., Düchs,
    D., Brunklaus, S., Hansen-Hagge, T., Meyer-Almes, F.-J., &#38; Drese, K. S. (2010).
    Compact, cost-efficient microfluidics-based stopped-flow device. <i>Analytical
    and Bioanalytical Chemistry</i>, <i>399</i>, 1117–1125. <a href="https://doi.org/10.1007/s00216-010-4446-5">https://doi.org/10.1007/s00216-010-4446-5</a>
  bjps: <b>Bleul R <i>et al.</i></b> (2010) Compact, Cost-Efficient Microfluidics-Based
    Stopped-Flow Device. <i>Analytical and bioanalytical chemistry</i> <b>399</b>,
    1117–1125.
  chicago: 'Bleul, Regina, Marion Ritzi-Lehnert, Julian Höth, Nico Scharpfenecker,
    Ines Frese, Dominik Düchs, Sabine Brunklaus, Thomas Hansen-Hagge, Franz-Josef
    Meyer-Almes, and Klaus S. Drese. “Compact, Cost-Efficient Microfluidics-Based
    Stopped-Flow Device.” <i>Analytical and Bioanalytical Chemistry</i> 399 (2010):
    1117–25. <a href="https://doi.org/10.1007/s00216-010-4446-5">https://doi.org/10.1007/s00216-010-4446-5</a>.'
  chicago-de: 'Bleul, Regina, Marion Ritzi-Lehnert, Julian Höth, Nico Scharpfenecker,
    Ines Frese, Dominik Düchs, Sabine Brunklaus, Thomas Hansen-Hagge, Franz-Josef
    Meyer-Almes und Klaus S. Drese. 2010. Compact, cost-efficient microfluidics-based
    stopped-flow device. <i>Analytical and bioanalytical chemistry</i> 399: 1117–1125.
    doi:<a href="https://doi.org/10.1007/s00216-010-4446-5">10.1007/s00216-010-4446-5</a>,
    .'
  din1505-2-1: '<span style="font-variant:small-caps;"><span style="font-variant:small-caps;">Bleul,
    Regina</span> ; <span style="font-variant:small-caps;">Ritzi-Lehnert, Marion</span>
    ; <span style="font-variant:small-caps;">Höth, Julian</span> ; <span style="font-variant:small-caps;">Scharpfenecker,
    Nico</span> ; <span style="font-variant:small-caps;">Frese, Ines</span> ; <span
    style="font-variant:small-caps;">Düchs, Dominik</span> ; <span style="font-variant:small-caps;">Brunklaus,
    Sabine</span> ; <span style="font-variant:small-caps;">Hansen-Hagge, Thomas</span>
    ; u. a.</span>: Compact, cost-efficient microfluidics-based stopped-flow device.
    In: <i>Analytical and bioanalytical chemistry</i> Bd. 399. Berlin, Springer (2010),
    S. 1117–1125'
  havard: R. Bleul, M. Ritzi-Lehnert, J. Höth, N. Scharpfenecker, I. Frese, D. Düchs,
    S. Brunklaus, T. Hansen-Hagge, F.-J. Meyer-Almes, K.S. Drese, Compact, cost-efficient
    microfluidics-based stopped-flow device, Analytical and Bioanalytical Chemistry.
    399 (2010) 1117–1125.
  ieee: 'R. Bleul <i>et al.</i>, “Compact, cost-efficient microfluidics-based stopped-flow
    device,” <i>Analytical and bioanalytical chemistry</i>, vol. 399, pp. 1117–1125,
    2010, doi: <a href="https://doi.org/10.1007/s00216-010-4446-5">10.1007/s00216-010-4446-5</a>.'
  mla: Bleul, Regina, et al. “Compact, Cost-Efficient Microfluidics-Based Stopped-Flow
    Device.” <i>Analytical and Bioanalytical Chemistry</i>, vol. 399, 2010, pp. 1117–25,
    <a href="https://doi.org/10.1007/s00216-010-4446-5">https://doi.org/10.1007/s00216-010-4446-5</a>.
  short: R. Bleul, M. Ritzi-Lehnert, J. Höth, N. Scharpfenecker, I. Frese, D. Düchs,
    S. Brunklaus, T. Hansen-Hagge, F.-J. Meyer-Almes, K.S. Drese, Analytical and Bioanalytical
    Chemistry 399 (2010) 1117–1125.
  ufg: '<b>Bleul, Regina u. a.</b>: Compact, cost-efficient microfluidics-based stopped-flow
    device, in: <i>Analytical and bioanalytical chemistry</i> 399 (2010),  S. 1117–1125.'
  van: Bleul R, Ritzi-Lehnert M, Höth J, Scharpfenecker N, Frese I, Düchs D, et al.
    Compact, cost-efficient microfluidics-based stopped-flow device. Analytical and
    bioanalytical chemistry. 2010;399:1117–25.
date_created: 2024-03-16T19:35:23Z
date_updated: 2024-06-26T13:55:06Z
doi: 10.1007/s00216-010-4446-5
intvolume: '       399'
language:
- iso: eng
page: 1117–1125
place: Berlin
publication: Analytical and bioanalytical chemistry
publication_identifier:
  eissn:
  - 1618-2650
  issn:
  - '1618-2642 '
publication_status: published
publisher: Springer
status: public
title: Compact, cost-efficient microfluidics-based stopped-flow device
type: scientific_journal_article
user_id: '65129'
volume: 399
year: '2010'
...