{"department":[{"_id":"DEP4010"}],"doi":"10.1111/trf.14617","user_id":"83781","issue":"7","volume":58,"title":"Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany","intvolume":" 58","language":[{"iso":"eng"}],"publication":"Transfusion","publication_status":"published","citation":{"mla":"Vollmer, Tanja, et al. “Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany.” Transfusion, vol. 58, no. 7, 2018, pp. 1654–64, https://doi.org/10.1111/trf.14617.","din1505-2-1":"Vollmer, Tanja ; Dabisch-Ruthe, Mareike ; Weinstock, Melanie ; Knabbe, Cornelius ; Dreier, Jens: Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. In: Transfusion Bd. 58. Oxford [u.a.] , Wiley-Blackwell (2018), Nr. 7, S. 1654–1664","ufg":"Vollmer, Tanja u. a.: Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany, in: Transfusion 58 (2018), H. 7, S. 1654–1664.","havard":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany, Transfusion. 58 (2018) 1654–1664.","short":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, J. Dreier, Transfusion 58 (2018) 1654–1664.","van":"Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion. 2018;58(7):1654–64.","chicago":"Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe, and Jens Dreier. “Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany.” Transfusion 58, no. 7 (2018): 1654–64. https://doi.org/10.1111/trf.14617.","apa":"Vollmer, T., Dabisch-Ruthe, M., Weinstock, M., Knabbe, C., & Dreier, J. (2018). Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion, 58(7), 1654–1664. https://doi.org/10.1111/trf.14617","ieee":"T. Vollmer, M. Dabisch-Ruthe, M. Weinstock, C. Knabbe, and J. Dreier, “Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany,” Transfusion, vol. 58, no. 7, pp. 1654–1664, 2018, doi: 10.1111/trf.14617.","chicago-de":"Vollmer, Tanja, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe und Jens Dreier. 2018. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion 58, Nr. 7: 1654–1664. doi:10.1111/trf.14617, .","ama":"Vollmer T, Dabisch-Ruthe M, Weinstock M, Knabbe C, Dreier J. Late sampling for automated culture to extend the platelet shelf life to 5 days in Germany. Transfusion. 2018;58(7):1654-1664. doi:10.1111/trf.14617","bjps":"Vollmer T et al. (2018) Late Sampling for Automated Culture to Extend the Platelet Shelf Life to 5 Days in Germany. Transfusion 58, 1654–1664."},"publication_identifier":{"issn":["0041-1132"],"eissn":["1537-2995"]},"place":"Oxford [u.a.] ","publisher":"Wiley-Blackwell","abstract":[{"lang":"eng","text":"BACKGROUND\r\nBacterial contamination of platelet concentrates (PCs) is still a major challenge in transfusion medicine. Different methodologic concepts and screening strategies have been developed and investigated concerning their usability. We evaluated the feasibility of BacT/ALERT automated culture (BacT/A, bioMérieux) with late sampling after 3 days at the earliest.\r\nSTUDY DESIGN AND METHODS\r\nTwenty-four bacterial strains isolated from PCs and six relevant strains from reference stocks were spiked into apheresis-derived PCs (10-60 colony-forming units [CFU]/bag). Sampling was performed after 3 days, and bacterial detection was investigated using the two detection methods (BacT/A and BactiFlow [BF], bioMérieux). The maximum time-to-result of BacT/A was set to less than 12 hours.\r\nRESULTS\r\nAll medium- or high-pathogenic strains are capable of proliferating to high titers, and 100% of contaminated samples were detected by BF and BacT/A (6 to ≤12 h incubation); lower detection rates of BacT/A were obtained within 6 hours of incubation (≤6 h: 76.2-93.4%). The majority of low-pathogenic isolates are also capable of growing in PCs (89.7%), showing a detection rate of 74.3% for BF versus 54.3% for BacT/A (6 to ≤12 h incubation). BacT/A failed to detect bacteria within 6 hours of incubation. Certainly, a small number of strains did not grow under PC storage conditions and were detectable by BacT/A only with increased detection times.\r\nCONCLUSIONS\r\nLate sampling after 3 days at the earliest, combined with reduced BacT/A incubation following the negative-to-date concept, offer an alternative opportunity to extend the shelf life of PCs from 4 to 5 days in Germany. The sensitivity of BacT/A with late sampling is nearly comparable to BF; the time-to-result is considerably longer.\r\n"}],"quality_controlled":"1","date_created":"2025-06-17T06:55:03Z","year":"2018","date_updated":"2025-06-17T13:48:11Z","author":[{"full_name":"Vollmer, Tanja","last_name":"Vollmer","first_name":"Tanja"},{"full_name":"Dabisch-Ruthe, Mareike","orcid":"https://orcid.org/0009-0008-7644-0826","id":"66516","first_name":"Mareike","last_name":"Dabisch-Ruthe"},{"first_name":"Melanie","last_name":"Weinstock","full_name":"Weinstock, Melanie"},{"last_name":"Knabbe","first_name":"Cornelius","full_name":"Knabbe, Cornelius"},{"first_name":"Jens","last_name":"Dreier","full_name":"Dreier, Jens"}],"page":"1654-1664","_id":"12988","status":"public","type":"scientific_journal_article"}