{"year":"2021","abstract":[{"text":"Bioprocess development and optimization are still cost- and time-intensive due to the enormous number of experiments involved. In this study, the recently introduced model-assisted Design of Experiments (mDoE) concept (Möller et al. in Bioproc Biosyst Eng 42(5):867, https://doi.org/10.1007/s00449-019-02089-7, 2019) was extended and implemented into a software (“mDoE-toolbox”) to significantly reduce the number of required cultivations. The application of the toolbox is exemplary shown in two case studies with Saccharomyces cerevisiae. In the first case study, a fed-batch process was optimized with respect to the pH value and linearly rising feeding rates of glucose and nitrogen source. Using the mDoE-toolbox, the biomass concentration was increased by 30% compared to previously performed experiments. The second case study was the whole-cell biocatalysis of ethyl acetoacetate (EAA) to (S)-ethyl-3-hydroxybutyrate (E3HB), for which the feeding rates of glucose, nitrogen source, and EAA were optimized. An increase of 80% compared to a previously performed experiment with similar initial conditions was achieved for the E3HB concentration.","lang":"eng"}],"quality_controlled":"1","place":"Berlin ; Heidelberg [u.a.]","title":"Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses","publication_identifier":{"isbn":["1615-7591"],"eissn":["1615-7605"]},"intvolume":"        44","publication_status":"published","date_updated":"2023-08-21T07:55:05Z","_id":"7986","department":[{"_id":"DEP4021"}],"user_id":"83781","volume":44,"citation":{"ufg":"<b>Moser, André u. a.</b>: Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses, in: <i>Bioprocess and Biosystems Engineering</i> 44 (2021), H. 4,  S. 683–700.","chicago-de":"Moser, André, Kim B. Kuchemüller, Sahar Deppe, Tanja Hernández Rodriguez, Björn Frahm, Ralf Pörtner, Volker C. Hass und Johannes Möller. 2021. Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses. <i>Bioprocess and Biosystems Engineering</i> 44, Nr. 4: 683–700. doi:<a href=\"https://doi.org/10.1007/s00449-020-02478-3\">10.1007/s00449-020-02478-3</a>, .","short":"A. Moser, K.B. Kuchemüller, S. Deppe, T. Hernández Rodriguez, B. Frahm, R. Pörtner, V.C. Hass, J. Möller, Bioprocess and Biosystems Engineering 44 (2021) 683–700.","chicago":"Moser, André, Kim B. Kuchemüller, Sahar Deppe, Tanja Hernández Rodriguez, Björn Frahm, Ralf Pörtner, Volker C. Hass, and Johannes Möller. “Model-Assisted DoE Software: Optimization of Growth and Biocatalysis in Saccharomyces Cerevisiae Bioprocesses.” <i>Bioprocess and Biosystems Engineering</i> 44, no. 4 (2021): 683–700. <a href=\"https://doi.org/10.1007/s00449-020-02478-3\">https://doi.org/10.1007/s00449-020-02478-3</a>.","mla":"Moser, André, et al. “Model-Assisted DoE Software: Optimization of Growth and Biocatalysis in Saccharomyces Cerevisiae Bioprocesses.” <i>Bioprocess and Biosystems Engineering</i>, vol. 44, no. 4, 2021, pp. 683–700, <a href=\"https://doi.org/10.1007/s00449-020-02478-3\">https://doi.org/10.1007/s00449-020-02478-3</a>.","havard":"A. Moser, K.B. Kuchemüller, S. Deppe, T. Hernández Rodriguez, B. Frahm, R. Pörtner, V.C. Hass, J. Möller, Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses, Bioprocess and Biosystems Engineering. 44 (2021) 683–700.","van":"Moser A, Kuchemüller KB, Deppe S, Hernández Rodriguez T, Frahm B, Pörtner R, et al. Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses. Bioprocess and Biosystems Engineering. 2021;44(4):683–700.","apa":"Moser, A., Kuchemüller, K. B., Deppe, S., Hernández Rodriguez, T., Frahm, B., Pörtner, R., Hass, V. C., &#38; Möller, J. (2021). Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses. <i>Bioprocess and Biosystems Engineering</i>, <i>44</i>(4), 683–700. <a href=\"https://doi.org/10.1007/s00449-020-02478-3\">https://doi.org/10.1007/s00449-020-02478-3</a>","bjps":"<b>Moser A <i>et al.</i></b> (2021) Model-Assisted DoE Software: Optimization of Growth and Biocatalysis in Saccharomyces Cerevisiae Bioprocesses. <i>Bioprocess and Biosystems Engineering</i> <b>44</b>, 683–700.","din1505-2-1":"<span style=\"font-variant:small-caps;\">Moser, André</span> ; <span style=\"font-variant:small-caps;\">Kuchemüller, Kim B.</span> ; <span style=\"font-variant:small-caps;\">Deppe, Sahar</span> ; <span style=\"font-variant:small-caps;\">Hernández Rodriguez, Tanja</span> ; <span style=\"font-variant:small-caps;\">Frahm, Björn</span> ; <span style=\"font-variant:small-caps;\">Pörtner, Ralf</span> ; <span style=\"font-variant:small-caps;\">Hass, Volker C.</span> ; <span style=\"font-variant:small-caps;\">Möller, Johannes</span>: Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses. In: <i>Bioprocess and Biosystems Engineering</i> Bd. 44. Berlin ; Heidelberg [u.a.], Springer (2021), Nr. 4, S. 683–700","ieee":"A. Moser <i>et al.</i>, “Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses,” <i>Bioprocess and Biosystems Engineering</i>, vol. 44, no. 4, pp. 683–700, 2021, doi: <a href=\"https://doi.org/10.1007/s00449-020-02478-3\">10.1007/s00449-020-02478-3</a>.","ama":"Moser A, Kuchemüller KB, Deppe S, et al. Model-assisted DoE software: Optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses. <i>Bioprocess and Biosystems Engineering</i>. 2021;44(4):683-700. doi:<a href=\"https://doi.org/10.1007/s00449-020-02478-3\">10.1007/s00449-020-02478-3</a>"},"type":"scientific_journal_article","date_created":"2022-05-05T13:10:11Z","keyword":["Biocatalysis","Monte Carlo methods","Fed-batch strategy","Model-assisted design of experiments","Quality by design"],"doi":"10.1007/s00449-020-02478-3","status":"public","publisher":"Springer","page":"683-700","author":[{"first_name":"André","last_name":"Moser","full_name":"Moser, André"},{"last_name":"Kuchemüller","full_name":"Kuchemüller, Kim B.","first_name":"Kim B."},{"full_name":"Deppe, Sahar","last_name":"Deppe","first_name":"Sahar"},{"last_name":"Hernández Rodriguez","full_name":"Hernández Rodriguez, Tanja","id":"52466","first_name":"Tanja"},{"last_name":"Frahm","full_name":"Frahm, Björn","first_name":"Björn","id":"45666"},{"first_name":"Ralf","last_name":"Pörtner","full_name":"Pörtner, Ralf"},{"full_name":"Hass, Volker C.","last_name":"Hass","first_name":"Volker C."},{"first_name":"Johannes","full_name":"Möller, Johannes","last_name":"Möller"}],"language":[{"iso":"eng"}],"issue":"4","publication":"Bioprocess and Biosystems Engineering"}