Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study
T. Hernández Rodriguez, S. Morerod, R. Pörtner, F.M. Wurm, B. Frahm, Processes 9 (2021).
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Abstract
<jats:p>concentration is an important objective. The phenotype of the cells in a reactor plays an important role. Are clonal cell populations showing high cell-specific growth rates more favorable than cell lines with higher cell-specific productivities or vice versa? Five clonal Chinese hamster ovary cell populations were analyzed based on the data of a 3-month-stability study. We adapted a mechanistic cell culture model to the experimental data of one such clonally derived cell population. Uncertainties and prior knowledge concerning model parameters were considered using Bayesian parameter estimations. This model was used then to define an inoculum train protocol. Based on this, we subsequently simulated the impacts of differences in growth rates (±10%) and production rates (±10% and ±50%) on the overall cultivation time, including making the inoculum train cultures; the final production phase, the volumetric titer in that bioreactor and the ratio of both, defined as overall process productivity. We showed thus unequivocally that growth rates have a higher impact (up to three times) on overall process productivity and for product output per year, whereas cells with higher productivity can potentially generate higher product concentrations in the production vessel.
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Zeitschriftentitel
Processes
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9
Zeitschriftennummer
6
Artikelnummer
964
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Hernández Rodriguez T, Morerod S, Pörtner R, Wurm FM, Frahm B. Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study. Processes. 2021;9(6). doi:10.3390/pr9060964
Hernández Rodriguez, T., Morerod, S., Pörtner, R., Wurm, F. M., & Frahm, B. (2021). Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study. Processes, 9(6), Article 964. https://doi.org/10.3390/pr9060964
Hernández Rodriguez T et al. (2021) Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study. Processes 9.
Hernández Rodriguez, Tanja, Sophie Morerod, Ralf Pörtner, Florian M. Wurm, and Björn Frahm. “Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study.” Processes 9, no. 6 (2021). https://doi.org/10.3390/pr9060964.
Hernández Rodriguez, Tanja, Sophie Morerod, Ralf Pörtner, Florian M. Wurm und Björn Frahm. 2021. Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study. Processes 9, Nr. 6. doi:10.3390/pr9060964, .
Hernández Rodriguez, Tanja ; Morerod, Sophie ; Pörtner, Ralf ; Wurm, Florian M. ; Frahm, Björn: Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study. In: Processes Bd. 9. Basel, MDPI AG (2021), Nr. 6
T. Hernández Rodriguez, S. Morerod, R. Pörtner, F.M. Wurm, B. Frahm, Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study, Processes. 9 (2021).
T. Hernández Rodriguez, S. Morerod, R. Pörtner, F. M. Wurm, and B. Frahm, “Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study,” Processes, vol. 9, no. 6, Art. no. 964, 2021, doi: 10.3390/pr9060964.
Hernández Rodriguez, Tanja, et al. “Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study.” Processes, vol. 9, no. 6, 964, 2021, https://doi.org/10.3390/pr9060964.
Hernández Rodriguez, Tanja u. a.: Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study, in: Processes 9 (2021), H. 6.
Hernández Rodriguez T, Morerod S, Pörtner R, Wurm FM, Frahm B. Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study. Processes. 2021;9(6).